RLG00000024452

protease binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002212
Physical Location & Seq
Forward (+)
35961186 .. 35962688
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024452

Sequence Viewer

Length: 1503 bp
ATGATTGCCATTGACTCCGAGATTGAAGAACCAATGAAACTGGAGAGTAAGGTAGAGAAAAGTAATTGCCTGGAAGAAGAAATCATGTTAGCAGAGCAATTGAAGCATGAGGAGAGATCAATGGAAGCATCATCATGTCAAATGGCTACTGAATTGAAATGTCAGCAGAGAGATATAGTCGCTGCAAATATTTTGGTCCAGATGGCCAATGAAGATTCTGCTTTGCTTCATCACACATTAAGTTTGCATGGAGTGAAGCGTAGCATGAAAGGGAAGCCGAGAATGAGGTGGGAAACGAAAGGTGATAGAATAGGAGTGGGTGATAATGATTCCAAAGTGGCTAGGAGAGGGGCCAAGTTTATTGCAAAGCGTCAGCAAGCGGCGTTTGGAGGTCAGACGAATGATGCAGATAGTGTAGTCTTAGCTTTATCAGCAGTGCCATGTTGTGGAAGGAGAAAGAACAGAAAGATGAACATGAGCATGGAACTGTCTTCAGTCCAACCAAATTATATAAATACTAATTTCAGTGATAACAAGGATGACAATTTATGTGAAGCGTGTGGAGATTTGGATGGTGATGAGCTGACTAGTAGATGGGGAAACGATCATCGCAAGTCATTTAAAGCTTGTGTTGAGGATTGTCATGATAGATTAGGTGTGTTGAGAGAGAAAAATGTGGATGTTGCAGGCTCAGTAAACATACCAAGACATGTGAAATGTACTGTCAGTGAGGAGGAGGATGACAATTTGTACGAGGCACGGCAGTTTTTGGATGGTCATGAGCTGACTGAGAGGTGGGGAAACAATGATCAGAAATCAGTTGAAGCTGGTCTTTGGGACCGTCATGATAGATTAGGCATGTTAAGTATAAAGAAGAGGAAAAGAGTGGCAGGATCCTACTCTAAAAGAGATGCCGAGGATGTTGCAGTACCAGGGAAAATACCATTGAAAATGCCCTTGAAATTGGCAGTTCCTATTGTTGAGAGACTTCATCCAGTTGGAAGCCCTTCTGCACCACCTCTGGTTTTGATGAGACCAACATTAAACAAAAGGAAAAGGTTTAGGGGTGTGGGCCGTAAGAGGAGCATCGCAACTATCAGCCCAAGCTCTGAAGAAGTAAGAGCTTACTTTCATAATGAGGAAGCTTTGAGGTACTTAGTCCCCGATAGGGGGTTTTGTTACACAGCACTGGATGGTAGAAAGTCTGCAGTCGCTCCTCTACGGAAGGTGAGCAACATACACCGAGACCATGTTATGCTTAAAGCTGATAGGCCGCGTGTTGTTTCCGTCTGCTGTCTTGTGCGGGATGCAGCTGCTAGATTGCCTGGAGGTATGGGTACCAGAGCAGATATATGTACTCTCATGCGGGACTCACAGTATCTAGTCGAAGATGTCACTGACGAACAACTTCACCATTTTGCTAGCAAAGGACTGGACAGGCTGCACTATGAAGTTGATCCCTGCGTCCAGTATGATGGGAAGTTCTGGGTTTATTTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

501

Amino Acids

56.42

Weight (kDa)

8.4

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 374 - 500 3e-50 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1337
AccB1I GGYRCC 1 cut(s) 1337
AccB7I CCANNNNNTGG 1 cut(s) 446
AccII CGCG 1 cut(s) 1276
AciI CCGC 4 cut(s) 380, 1274, 1303, 1366
AclWI GGATC 3 cut(s) 888, 901, 1451
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 2 cut(s) 477, 1131
AfaI GTAC 6 cut(s) 721, 752, 930, 1154, 1339, 1357
AfiI CCNNNNNNNGG 4 cut(s) 446, 1168, 1169, 1170
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 6 cut(s) 26, 103, 157, 824, 949, 961
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 3 cut(s) 69, 931, 1324
Alw26I GTCTC 3 cut(s) 979, 1027, 1239
AlwI GGATC 3 cut(s) 888, 901, 1451
AoxI GGCC 4 cut(s) 204, 351, 1072, 1271
ApeKI GCWGC 4 cut(s) 182, 1310, 1313, 1441
Asp700I GAANNNNTTC 2 cut(s) 1006, 1407
Asp718I GGTACC 1 cut(s) 1337
AspS9I GGNCC 4 cut(s) 196, 351, 838, 1072
AsuHPI GGTGA 5 cut(s) 314, 332, 587, 1240, 1403
AsuNHI GCTAGC 1 cut(s) 1421
AvaII GGWCC 2 cut(s) 196, 838
BalI TGGCCA 1 cut(s) 206
BamHI GGATCC 1 cut(s) 893
BanI GGYRCC 1 cut(s) 1337
BbsI GAAGAC 1 cut(s) 483
BbvI GCAGC 4 cut(s) 169, 1300, 1322, 1428
BccI CCATC 6 cut(s) 196, 566, 588, 767, 1187, 1469
BceAI ACGGC 2 cut(s) 776, 1059
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciT130I CCWGG 3 cut(s) 71, 933, 1326
BclI TGATCA 1 cut(s) 808
BcoDI GTCTC 3 cut(s) 979, 1027, 1239
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 5 cut(s) 342, 588, 1317, 1382, 1422
BfmI CTRYAG 1 cut(s) 1206
BisI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
BlsI GCNGC 6 cut(s) 184, 382, 1275, 1312, 1315, 1443
Bme1390I CCNGG 3 cut(s) 71, 933, 1326
Bme18I GGWCC 2 cut(s) 196, 838
BmgT120I GGNCC 4 cut(s) 196, 351, 838, 1072
BmiI GGNNCC 4 cut(s) 352, 839, 895, 1339
BmrFI CCNGG 3 cut(s) 71, 933, 1326
BmsI GCATC 5 cut(s) 137, 394, 901, 1095, 1297
BmtI GCTAGC 1 cut(s) 1425
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 2 cut(s) 62, 1347
BsaI GGTCTC 2 cut(s) 1027, 1239
BsaJI CCNNGG 2 cut(s) 915, 932
Bsc4I CCNNNNNNNGG 4 cut(s) 446, 1168, 1169, 1170
Bse1I ACTGG 5 cut(s) 45, 995, 1194, 1437, 1468
BseBI CCWGG 3 cut(s) 71, 933, 1326
BseDI CCNNGG 2 cut(s) 915, 932
BseGI GGATG 9 cut(s) 544, 577, 685, 745, 778, 925, 991, 1198, 1312
BseLI CCNNNNNNNGG 4 cut(s) 446, 1168, 1169, 1170
BseMII CTCAG 2 cut(s) 705, 780
BseNI ACTGG 5 cut(s) 45, 995, 1194, 1437, 1468
BseRI GAGGAG 5 cut(s) 125, 746, 749, 1096, 1206
BseXI GCAGC 4 cut(s) 169, 1300, 1322, 1428
BsgI GTGCAG 2 cut(s) 996, 1427
Bsh1236I CGCG 1 cut(s) 1276
BshFI GGCC 4 cut(s) 206, 353, 1074, 1273
BshNI GGYRCC 1 cut(s) 1337
BslFI GGGAC 3 cut(s) 851, 1145, 1382
BslI CCNNNNNNNGG 4 cut(s) 446, 1168, 1169, 1170
BsmAI GTCTC 3 cut(s) 979, 1027, 1239
BsmFI GGGAC 3 cut(s) 851, 1145, 1382
BsnI GGCC 4 cut(s) 206, 353, 1074, 1273
Bso31I GGTCTC 2 cut(s) 1027, 1239
Bsp143I GATC 5 cut(s) 116, 604, 808, 893, 1456
BspACI CCGC 4 cut(s) 380, 1274, 1303, 1366
BspANI GGCC 4 cut(s) 206, 353, 1074, 1273
BspCNI CTCAG 2 cut(s) 704, 781
BspFNI CGCG 1 cut(s) 1276
BspHI TCATGA 3 cut(s) 643, 778, 844
BspLI GGNNCC 4 cut(s) 352, 839, 895, 1339
BspMAI CTGCAG 1 cut(s) 1210
BspOI GCTAGC 1 cut(s) 1425
BspPI GGATC 3 cut(s) 888, 901, 1451
BspT107I GGYRCC 1 cut(s) 1337
BspTNI GGTCTC 2 cut(s) 1027, 1239
BsrI ACTGG 5 cut(s) 45, 995, 1194, 1437, 1468
BssECI CCNNGG 2 cut(s) 915, 932
BssMI GATC 5 cut(s) 116, 604, 808, 893, 1456
Bst2UI CCWGG 3 cut(s) 71, 933, 1326
Bst4CI ACNGT 4 cut(s) 489, 724, 842, 1377
Bst6I CTCTTC 1 cut(s) 869
BstC8I GCNNGC 3 cut(s) 378, 688, 1423
BstDEI CTNAG 4 cut(s) 421, 691, 789, 1156
BstF5I GGATG 9 cut(s) 544, 577, 685, 745, 778, 925, 991, 1198, 1312
BstFNI CGCG 1 cut(s) 1276
BstKTI GATC 5 cut(s) 119, 607, 811, 896, 1459
BstMAI GTCTC 3 cut(s) 979, 1027, 1239
BstMBI GATC 5 cut(s) 116, 604, 808, 893, 1456
BstMWI GCNNNNNNNGC 2 cut(s) 103, 431
BstNI CCWGG 3 cut(s) 71, 933, 1326
BstNSI RCATGY 2 cut(s) 713, 862
BstSCI CCNGG 3 cut(s) 69, 931, 1324
BstSFI CTRYAG 1 cut(s) 1206
BstUI CGCG 1 cut(s) 1276
BstV1I GCAGC 4 cut(s) 169, 1300, 1322, 1428
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 893
BstXI CCANNNNNNTGG 1 cut(s) 1475
BstYI RGATCY 1 cut(s) 893
BsuRI GGCC 4 cut(s) 206, 353, 1074, 1273
BtgZI GCGATG 2 cut(s) 593, 1072
BtsCI GGATG 9 cut(s) 544, 577, 685, 745, 778, 925, 991, 1198, 1312
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 5 cut(s) 441, 532, 733, 1187, 1395
Cac8I GCNNGC 3 cut(s) 378, 688, 1423
CciI TCATGA 3 cut(s) 643, 778, 844
Cfr13I GGNCC 4 cut(s) 196, 351, 838, 1072
CseI GACGC 2 cut(s) 359, 1453
Csp6I GTAC 6 cut(s) 720, 751, 929, 1153, 1338, 1356
CviQI GTAC 6 cut(s) 720, 751, 929, 1153, 1338, 1356
DdeI CTNAG 4 cut(s) 421, 691, 789, 1156
DpnI GATC 5 cut(s) 118, 606, 810, 895, 1458
DpnII GATC 5 cut(s) 116, 604, 808, 893, 1456
DraI TTTAAA 1 cut(s) 622
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 869
EarI CTCTTC 1 cut(s) 869
Eco31I GGTCTC 2 cut(s) 1027, 1239
Eco47I GGWCC 2 cut(s) 196, 838
Eco57I CTGAAG 2 cut(s) 477, 1131
EcoRII CCWGG 3 cut(s) 69, 931, 1324
FalI AAGNNNNNCTT 2 cut(s) 816, 848
FaqI GGGAC 3 cut(s) 851, 1145, 1382
FauI CCCGC 2 cut(s) 1296, 1359
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
FokI GGATG 9 cut(s) 551, 584, 692, 752, 785, 932, 978, 1205, 1319
Fsp4HI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
FspBI CTAG 5 cut(s) 342, 588, 1317, 1382, 1422
GluI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
GsuI CTGGAG 2 cut(s) 62, 1347
HaeIII GGCC 4 cut(s) 206, 353, 1074, 1273
HgaI GACGC 2 cut(s) 359, 1453
HindIII AAGCTT 2 cut(s) 624, 1143
HinfI GANTC 4 cut(s) 14, 215, 329, 1370
HphI GGTGA 5 cut(s) 314, 332, 587, 1240, 1403
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 4 cut(s) 19, 396, 813, 1111
Hpy188III TCNNGA 4 cut(s) 199, 644, 779, 845
Hpy8I GTNNAC 1 cut(s) 697
HpyAV CCTTC 3 cut(s) 444, 1017, 1219
HpyCH4III ACNGT 4 cut(s) 489, 724, 842, 1377
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 431
HpyF3I CTNAG 4 cut(s) 421, 691, 789, 1156
KpnI GGTACC 1 cut(s) 1341
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 5 cut(s) 116, 604, 808, 893, 1456
LmnI GCTCC 2 cut(s) 1083, 1219
Lsp1109I GCAGC 4 cut(s) 169, 1300, 1322, 1428
LweI GCATC 5 cut(s) 137, 394, 901, 1095, 1297
MaeI CTAG 5 cut(s) 342, 588, 1317, 1382, 1422
MaeIII GTNAC 2 cut(s) 1178, 1393
MalI GATC 5 cut(s) 118, 606, 810, 895, 1458
MboI GATC 5 cut(s) 116, 604, 808, 893, 1456
MboII GAAGA 8 cut(s) 38, 86, 89, 224, 483, 886, 1124, 1400
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 1 cut(s) 893
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 8 cut(s) 64, 98, 152, 505, 520, 544, 745, 962
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 2 cut(s) 8, 1364
MmeI TCCRAC 2 cut(s) 523, 979
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 2 cut(s) 1006, 1407
MscI TGGCCA 1 cut(s) 206
MseI TTAA 5 cut(s) 239, 621, 863, 1043, 1260
MslI CAYNNNNRTG 2 cut(s) 133, 479
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 1313
MspR9I CCNGG 3 cut(s) 71, 933, 1326
MunI CAATTG 1 cut(s) 98
MvaI CCWGG 3 cut(s) 71, 933, 1326
MvnI CGCG 1 cut(s) 1276
MwoI GCNNNNNNNGC 2 cut(s) 103, 431
NdeII GATC 5 cut(s) 116, 604, 808, 893, 1456
NheI GCTAGC 1 cut(s) 1421
NlaIV GGNNCC 4 cut(s) 352, 839, 895, 1339
NmeAIII GCCGAG 2 cut(s) 303, 940
NmuCI GTSAC 1 cut(s) 1393
NspI RCATGY 2 cut(s) 713, 862
PagI TCATGA 3 cut(s) 643, 778, 844
PciI ACATGT 1 cut(s) 709
PdmI GAANNNNTTC 2 cut(s) 1006, 1407
PfeI GAWTC 2 cut(s) 215, 329
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 6 cut(s) 184, 382, 1275, 1312, 1315, 1443
PleI GAGTC 2 cut(s) 8, 1364
PpsI GAGTC 2 cut(s) 8, 1364
PscI ACATGT 1 cut(s) 709
Psp6I CCWGG 3 cut(s) 69, 931, 1324
PspGI CCWGG 3 cut(s) 69, 931, 1324
PspN4I GGNNCC 4 cut(s) 352, 839, 895, 1339
PspPI GGNCC 4 cut(s) 196, 351, 838, 1072
PstI CTGCAG 1 cut(s) 1210
PsuI RGATCY 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 1313
RsaI GTAC 6 cut(s) 721, 752, 930, 1154, 1339, 1357
RsaNI GTAC 6 cut(s) 720, 751, 929, 1153, 1338, 1356
RseI CAYNNNNRTG 2 cut(s) 133, 479
SaqAI TTAA 5 cut(s) 239, 621, 863, 1043, 1260
SatI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
Sau3AI GATC 5 cut(s) 116, 604, 808, 893, 1456
Sau96I GGNCC 4 cut(s) 196, 351, 838, 1072
SchI GAGTC 2 cut(s) 8, 1364
ScrFI CCNGG 3 cut(s) 71, 933, 1326
SfaNI GCATC 5 cut(s) 137, 394, 901, 1095, 1297
SfcI CTRYAG 1 cut(s) 1206
SinI GGWCC 2 cut(s) 196, 838
SmiMI CAYNNNNRTG 2 cut(s) 133, 479
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 8 cut(s) 64, 98, 152, 505, 520, 544, 745, 962
SsiI CCGC 4 cut(s) 380, 1274, 1303, 1366
SspI AATATT 1 cut(s) 190
SspMI CTAG 5 cut(s) 342, 588, 1317, 1382, 1422
StyD4I CCNGG 3 cut(s) 69, 931, 1324
TaaI ACNGT 4 cut(s) 489, 724, 842, 1377
TaqI TCGA 1 cut(s) 1386
TasI AATT 8 cut(s) 64, 98, 152, 505, 520, 544, 745, 962
TatI WGTACW 2 cut(s) 719, 1355
TauI GCSGC 2 cut(s) 383, 1276
TfiI GAWTC 2 cut(s) 215, 329
Tru1I TTAA 5 cut(s) 239, 621, 863, 1043, 1260
Tru9I TTAA 5 cut(s) 239, 621, 863, 1043, 1260
TscAI CASTG 5 cut(s) 441, 532, 733, 1194, 1402
TseFI GTSAC 1 cut(s) 1393
TseI GCWGC 4 cut(s) 182, 1310, 1313, 1441
Tsp45I GTSAC 1 cut(s) 1393
TspDTI ATGAA 8 cut(s) 50, 218, 225, 281, 485, 980, 1121, 1464
TspGWI ACGGA 2 cut(s) 1237, 1276
TspRI CASTG 5 cut(s) 441, 532, 733, 1194, 1402
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 196, 838
XceI RCATGY 2 cut(s) 713, 862
XmnI GAANNNNTTC 2 cut(s) 1006, 1407
XspI CTAG 5 cut(s) 342, 588, 1317, 1382, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.