Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002212

Physical Location & Seq

Genomic Coordinates Forward (+)

20538519 .. 20540012

Gene Structure 1494 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1130300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGAATGGAGTTCTCCTTCAAGCCTGTGTCAGTTTCAGGAAGAAACTTTCTCAGTCCGTTCTGCAAACACATTCATCTCACTCCAAGCCCTTCTTCTCTTCTACCAAATTCACACGAACAACCCAAAAAACCAACAAGCTACAAAACTCTCTCAGAGTTATCGACCTCATCACCCCAAAACCACCCCCCGCCGCGCGCCGCCGCCGAGGCCATCTCCGGCTCATTCAGGACTTTCTGCAGTCAGATTCCGACCACTTTACCGTCGGTTCTGCTTCCTCTGATCCAACAAACCCACGAGTAAAAATCTCAACTTTGTTCGATGAAAGCGTTTTGTCACACGCCATAAGCTCATGTGGGTCTTCTCGAAATCTTCATGGCGGGGTTCAGTACCACTGTGCAGCAATGAGAATTGGGTTTATTTCCAATGTTTATATTGGAAGCTCTTTGGTGAGTTTGTACAGCCGATGCGGTGAGCTGCAGAGTGCATATAAGTTGTTCGACGAAATGCCTGTGAGAAATGTGGTGTCTTGGACTGCCATTATTTCCGGTTTTGCGCAAGCGTGGCAGGTTGATGTGTGCTTGCAGCTTTTCAGTGAAATGAGAAACTTTTTGGAACCGAATGATTTTACGTATGCAAGTATTCTGAGTGCTTGTACTGGCAGTGGAGCTCTTGGGCATGGAAGAAGTGCTCATTGCCAAACCATCCGAACAGGCTTCAATTCGTACATCCACATTGCTAATGCTCTTATCTCAATGTATTGCAAGTGTGGGGATGTTAAAGATGCGCTTTCCATATTCGAAAATTTGGATGGCAGAGATAATGTATCTTGGAACTCCATGATTGCCGGGTATGCGCAGCATGGGCTTGCATTGCAGGCCATTGATCTTTTTGAAGAAATGAAGCGGCAACGTGTGAAACCTGATGCCATTACTTTATTGGGCGTGCTCTCTTCGTGTCGTCATGCAGGTCTTGTCAAAGAAGGCCGGCTTTACTTCAATTCAATGATCAAAGAGCATGGTATTCATCCAGAGCTAGATCATTATTCGTGTGTTGTTGACCTTCTTGGCCGCGCTGGTCTATTAGAAGAGGCTCGAGAATTCATTGAACAGATGCCTATTCGTGCCAACGCAGTTATATGGGGCTCGCTGCTGTCATCCTGTAGGGTTTATGGGAGTGTGTGGATTGGCATTGAGGCTGCGGAGAGTAGGCTATTACTAGAGCCAAAGTGTGCTTCAACCCATGTGCAATTGGCAAACCTGTATGCTAGTGTGGGATGTTGGGATGGGGCAGCAAGAATGCGAAAGTTGATGAAAGATAAAGCGATTAGGACAAGTCCTGGGTTCAGCTGGATTGCGATTGGAAATGAAGTTCACAAATTTAGAGCAGAAGACTGTTCAAATCCCAAAATGATTGAAATTATTGATCTCTTGGATAGTTTGGCAGACCACATTAAAACTTTAGCCTATGAGCCTGAAATGGAAGAGGAAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

498

Amino Acids

55.22

Weight (kDa)

7.89

Isoelectric Point (pI)

48.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	172 - 218	1.9e-09	PPR repeat family
PPR_2	PF13041	273 - 318	3.9e-11	PPR repeat family
PPR	PF01535	275 - 304	1.1e-08	PPR repeat
E_motif	PF20431	395 - 451	2.7e-10	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002212)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37320
fragaria_vesca	FvH4_6g14840 FvH4_6g14850 FvH4_6g14860 FvH4_6g14860 FvH4_6g14860
malus_domestica	MD12G1130300.v1.1
prunus_persica	Prupe.6G245300_v2.0.a1 Prupe.6G245400_v2.0.a1 Prupe.6G245400_v2.0.a1
pyrus_communis	pycom12g12640
rosa_chinensis	RchiOBHm_Chr3g0468071
rosa_laevigata	RLG00000024452 RLG00000024453 RLG00000024454
rosa_multiflora	Rmu_co8076902.1_g000001 Rmu_co8076904.1_g000001 Rmu_co8172106.1_g000001 Rmu_co8309729.1_g000001 Rmu_sc0005326.1_g000004 Rmu_sc0012651.1_g000004 Rmu_sc0012651.1_g000005
rosa_roxburghii	Rroxscaffold_6G00413080 Rroxscaffold_6G00413090 Rroxscaffold_6G00413100
rosa_rugosa	Rorug03G0092100 Rorug03G0092200 Rorug03G0092300
rosa_samantha	Rh3AG140600 Rh3AG140700 Rh3AG140800 Rh3BG161900 Rh3BG162000 Rh3BG162100 Rh3BG162200 Rh3CG161900 Rh3CG162000 Rh3DG162400 Rh3DG162500 Rh3DG162600
rosa_wichuraiana	Rw3G013280 Rw3G013290 Rw3G013300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	555, 855
Acc36I	ACCTGC	2 cut(s)	556, 956
AccII	CGCG	3 cut(s)	194, 196, 1071
AciI	CCGC	9 cut(s)	189, 192, 199, 202, 378, 468, 904, 1069, 1199
AclWI	GGATC	1 cut(s)	275
AcoI	YGGCCR	1 cut(s)	1066
AcsI	RAATTY	4 cut(s)	107, 802, 1097, 1376
AfaI	GTAC	4 cut(s)	389, 458, 655, 725
AflIII	ACRYGT	1 cut(s)	910
AgsI	TTSAA	9 cut(s)	20, 718, 893, 997, 1002, 1106, 1236, 1398, 1415
AjnI	CCWGG	1 cut(s)	1336
AjuI	GAANNNNNNNTTGG	4 cut(s)	77, 109, 1216, 1248
AloI	GAACNNNNNNTCC	2 cut(s)	1353, 1385
AluBI	AGCT	8 cut(s)	139, 348, 441, 475, 586, 668, 1033, 1347
AluI	AGCT	8 cut(s)	139, 348, 441, 475, 586, 668, 1033, 1347
Alw21I	GWGCWC	3 cut(s)	670, 691, 948
AlwI	GGATC	1 cut(s)	275
Ama87I	CYCGRG	1 cut(s)	1092
AoxI	GGCC	4 cut(s)	208, 876, 982, 1066
ApeKI	GCWGC	7 cut(s)	398, 475, 583, 856, 1147, 1196, 1289
ApoI	RAATTY	4 cut(s)	107, 802, 1097, 1376
AspLEI	GCGC	6 cut(s)	196, 198, 556, 787, 856, 1073
AsuC2I	CCSGG	1 cut(s)	847
AsuHPI	GGTGA	3 cut(s)	163, 460, 482
AsuII	TTCGAA	1 cut(s)	798
AvaI	CYCGRG	1 cut(s)	1092
BanII	GRGCYC	2 cut(s)	670, 1145
BauI	CACGAG	1 cut(s)	294
BbsI	GAAGAC	2 cut(s)	351, 1395
Bbv12I	GWGCWC	3 cut(s)	670, 691, 948
BbvI	GCAGC	7 cut(s)	410, 462, 595, 868, 1134, 1183, 1301
BccI	CCATC	4 cut(s)	219, 710, 803, 1277
BciT130I	CCWGG	1 cut(s)	1338
BclI	TGATCA	1 cut(s)	1005
BcnI	CCSGG	1 cut(s)	847
BfaI	CTAG	3 cut(s)	1034, 1217, 1266
BfmI	CTRYAG	3 cut(s)	236, 476, 1159
BfuAI	ACCTGC	2 cut(s)	556, 956
BglI	GCCNNNNNGGC	1 cut(s)	207
Bme1390I	CCNGG	2 cut(s)	847, 1338
BmeT110I	CYCGRG	1 cut(s)	1092
BmiI	GGNNCC	1 cut(s)	615
BmrFI	CCNGG	2 cut(s)	847, 1338
BmsI	GCATC	4 cut(s)	455, 772, 913, 1101
BpiI	GAAGAC	2 cut(s)	351, 1395
BplI	GAGNNNNNCTC	2 cut(s)	198, 230
Bpu14I	TTCGAA	1 cut(s)	798
BpuMI	CCSGG	1 cut(s)	847
BsaAI	YACGTR	1 cut(s)	630
BsaJI	CCNNGG	2 cut(s)	205, 1337
BsaWI	WCCGGW	1 cut(s)	545
Bse118I	RCCGGY	1 cut(s)	984
Bse1I	ACTGG	1 cut(s)	661
Bse3DI	GCAATG	4 cut(s)	408, 691, 732, 869
BseBI	CCWGG	1 cut(s)	1338
BseDI	CCNNGG	2 cut(s)	205, 1337
BseGI	GGATG	8 cut(s)	702, 726, 778, 814, 1024, 1154, 1280, 1288
BseMI	GCAATG	4 cut(s)	408, 691, 732, 869
BseMII	CTCAG	3 cut(s)	65, 166, 635
BseNI	ACTGG	1 cut(s)	661
BsePI	GCGCGC	1 cut(s)	194
BseXI	GCAGC	7 cut(s)	410, 462, 595, 868, 1134, 1183, 1301
BsgI	GTGCAG	1 cut(s)	417
Bsh1236I	CGCG	3 cut(s)	194, 196, 1071
BshFI	GGCC	4 cut(s)	210, 878, 984, 1068
BsiHKAI	GWGCWC	3 cut(s)	670, 691, 948
BsiHKCI	CYCGRG	1 cut(s)	1092
BsiSI	CCGG	4 cut(s)	217, 546, 846, 985
BsmI	GAATGC	1 cut(s)	1302
BsnI	GGCC	4 cut(s)	210, 878, 984, 1068
BsoBI	CYCGRG	1 cut(s)	1092
Bsp119I	TTCGAA	1 cut(s)	798
Bsp1286I	GDGCHC	4 cut(s)	670, 691, 948, 1145
Bsp1407I	TGTACA	1 cut(s)	456
Bsp143I	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
BspACI	CCGC	9 cut(s)	189, 192, 199, 202, 378, 468, 904, 1069, 1199
BspANI	GGCC	4 cut(s)	210, 878, 984, 1068
BspCNI	CTCAG	3 cut(s)	64, 165, 636
BspFNI	CGCG	3 cut(s)	194, 196, 1071
BspLI	GGNNCC	1 cut(s)	615
BspMAI	CTGCAG	2 cut(s)	240, 480
BspMI	ACCTGC	2 cut(s)	556, 956
BspPI	GGATC	1 cut(s)	275
BspT104I	TTCGAA	1 cut(s)	798
BsrDI	GCAATG	4 cut(s)	408, 691, 732, 869
BsrFI	RCCGGY	1 cut(s)	984
BsrGI	TGTACA	1 cut(s)	456
BsrI	ACTGG	1 cut(s)	661
BssAI	RCCGGY	1 cut(s)	984
BssECI	CCNNGG	2 cut(s)	205, 1337
BssHII	GCGCGC	1 cut(s)	194
BssMI	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
BssSI	CACGAG	1 cut(s)	294
Bst2BI	CACGAG	1 cut(s)	294
Bst2UI	CCWGG	1 cut(s)	1338
Bst4CI	ACNGT	3 cut(s)	262, 395, 1394
Bst6I	CTCTTC	4 cut(s)	103, 955, 1080, 1476
BstAUI	TGTACA	1 cut(s)	456
BstBAI	YACGTR	1 cut(s)	630
BstBI	TTCGAA	1 cut(s)	798
BstC8I	GCNNGC	8 cut(s)	196, 558, 581, 867, 876, 944, 986, 1145
BstDEI	CTNAG	3 cut(s)	51, 152, 644
BstF5I	GGATG	8 cut(s)	702, 726, 778, 814, 1024, 1154, 1280, 1288
BstFNI	CGCG	3 cut(s)	194, 196, 1071
BstHHI	GCGC	6 cut(s)	196, 198, 556, 787, 856, 1073
BstKTI	GATC	5 cut(s)	283, 886, 1008, 1039, 1426
BstMBI	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
BstMWI	GCNNNNNNNGC	6 cut(s)	207, 562, 851, 862, 871, 875
BstNI	CCWGG	1 cut(s)	1338
BstSCI	CCNGG	2 cut(s)	845, 1336
BstSFI	CTRYAG	3 cut(s)	236, 476, 1159
BstSNI	TACGTA	1 cut(s)	630
BstUI	CGCG	3 cut(s)	194, 196, 1071
BstV1I	GCAGC	7 cut(s)	410, 462, 595, 868, 1134, 1183, 1301
BstV2I	GAAGAC	2 cut(s)	351, 1395
BsuRI	GGCC	4 cut(s)	210, 878, 984, 1068
BtsCI	GGATG	8 cut(s)	702, 726, 778, 814, 1024, 1154, 1280, 1288
BtsI	GCAGTG	1 cut(s)	667
BtsIMutI	CAGTG	3 cut(s)	391, 598, 667
BveI	ACCTGC	2 cut(s)	556, 956
Cac8I	GCNNGC	8 cut(s)	196, 558, 581, 867, 876, 944, 986, 1145
CfoI	GCGC	6 cut(s)	196, 198, 556, 787, 856, 1073
Cfr10I	RCCGGY	1 cut(s)	984
Csp6I	GTAC	4 cut(s)	388, 457, 654, 724
CviAII	CATG	8 cut(s)	351, 374, 677, 838, 860, 962, 1016, 1241
CviQI	GTAC	4 cut(s)	388, 457, 654, 724
DdeI	CTNAG	3 cut(s)	51, 152, 644
DpnI	GATC	5 cut(s)	282, 885, 1007, 1038, 1425
DpnII	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
EaeI	YGGCCR	1 cut(s)	1066
Eam1104I	CTCTTC	4 cut(s)	103, 955, 1080, 1476
EarI	CTCTTC	4 cut(s)	103, 955, 1080, 1476
Ecl136II	GAGCTC	1 cut(s)	668
Eco105I	TACGTA	1 cut(s)	630
Eco24I	GRGCYC	2 cut(s)	670, 1145
Eco53kI	GAGCTC	1 cut(s)	668
Eco88I	CYCGRG	1 cut(s)	1092
EcoICRI	GAGCTC	1 cut(s)	668
EcoRI	GAATTC	1 cut(s)	1097
EcoRII	CCWGG	1 cut(s)	1336
EcoT38I	GRGCYC	2 cut(s)	670, 1145
FaeI	CATG	8 cut(s)	354, 377, 680, 841, 863, 965, 1019, 1244
FalI	AAGNNNNNCTT	6 cut(s)	77, 109, 771, 803, 972, 1004
FatI	CATG	8 cut(s)	350, 373, 676, 837, 859, 961, 1015, 1240
FauI	CCCGC	2 cut(s)	196, 371
FbaI	TGATCA	1 cut(s)	1005
FokI	GGATG	8 cut(s)	689, 713, 785, 821, 1011, 1141, 1287, 1295
FriOI	GRGCYC	2 cut(s)	670, 1145
FspBI	CTAG	3 cut(s)	1034, 1217, 1266
FspI	TGCGCA	2 cut(s)	555, 855
GlaI	GCGC	6 cut(s)	195, 197, 555, 786, 855, 1072
HaeIII	GGCC	4 cut(s)	210, 878, 984, 1068
HapII	CCGG	4 cut(s)	217, 546, 846, 985
HhaI	GCGC	6 cut(s)	196, 198, 556, 787, 856, 1073
Hin1II	CATG	8 cut(s)	354, 377, 680, 841, 863, 965, 1019, 1244
Hin6I	GCGC	6 cut(s)	194, 196, 554, 785, 854, 1071
HinP1I	GCGC	6 cut(s)	194, 196, 554, 785, 854, 1071
HincII	GTYRAC	1 cut(s)	1057
HindII	GTYRAC	1 cut(s)	1057
HinfI	GANTC	1 cut(s)	245
HpaII	CCGG	4 cut(s)	217, 546, 846, 985
HphI	GGTGA	3 cut(s)	163, 460, 482
Hpy166II	GTNNAC	2 cut(s)	1057, 1372
Hpy188I	TCNGA	6 cut(s)	155, 244, 250, 280, 645, 707
Hpy188III	TCNNGA	5 cut(s)	37, 227, 363, 1028, 1094
Hpy8I	GTNNAC	2 cut(s)	1057, 1372
Hpy99I	CGWCG	2 cut(s)	266, 503
HpyAV	CCTTC	4 cut(s)	26, 100, 974, 1070
HpyCH4III	ACNGT	3 cut(s)	262, 395, 1394
HpyCH4IV	ACGT	2 cut(s)	629, 910
HpyF10VI	GCNNNNNNNGC	6 cut(s)	207, 562, 851, 862, 871, 875
HpyF3I	CTNAG	3 cut(s)	51, 152, 644
HpySE526I	ACGT	2 cut(s)	629, 910
Hsp92II	CATG	8 cut(s)	354, 377, 680, 841, 863, 965, 1019, 1244
HspAI	GCGC	6 cut(s)	194, 196, 554, 785, 854, 1071
KroI	GCCGGC	1 cut(s)	984
KroNI	GCCGGC	1 cut(s)	986
Ksp22I	TGATCA	1 cut(s)	1005
Kzo9I	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
LmnI	GCTCC	1 cut(s)	665
Lsp1109I	GCAGC	7 cut(s)	410, 462, 595, 868, 1134, 1183, 1301
LweI	GCATC	4 cut(s)	455, 772, 913, 1101
MaeI	CTAG	3 cut(s)	1034, 1217, 1266
MaeII	ACGT	2 cut(s)	629, 910
MaeIII	GTNAC	1 cut(s)	333
MalI	GATC	5 cut(s)	282, 885, 1007, 1038, 1425
MboI	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
MfeI	CAATTG	1 cut(s)	1247
MhlI	GDGCHC	4 cut(s)	670, 691, 948, 1145
MluCI	AATT	9 cut(s)	107, 408, 718, 802, 997, 1097, 1247, 1376, 1416
MmeI	TCCRAC	2 cut(s)	273, 308
MnlI	CCTC	6 cut(s)	176, 200, 286, 1081, 1186, 1477
MroNI	GCCGGC	1 cut(s)	984
MseI	TTAA	3 cut(s)	777, 1452, 1492
MspA1I	CMGCKG	1 cut(s)	1347
MspI	CCGG	4 cut(s)	217, 546, 846, 985
MspR9I	CCNGG	2 cut(s)	847, 1338
MunI	CAATTG	1 cut(s)	1247
Mva1269I	GAATGC	1 cut(s)	1302
MvaI	CCWGG	1 cut(s)	1338
MvnI	CGCG	3 cut(s)	194, 196, 1071
MwoI	GCNNNNNNNGC	6 cut(s)	207, 562, 851, 862, 871, 875
NaeI	GCCGGC	1 cut(s)	986
NciI	CCSGG	1 cut(s)	847
NdeII	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
NgoMIV	GCCGGC	1 cut(s)	984
NlaIII	CATG	8 cut(s)	354, 377, 680, 841, 863, 965, 1019, 1244
NlaIV	GGNNCC	1 cut(s)	615
NmeAIII	GCCGAG	1 cut(s)	230
NmuCI	GTSAC	1 cut(s)	333
NsbI	TGCGCA	2 cut(s)	555, 855
NspV	TTCGAA	1 cut(s)	798
PaeR7I	CTCGAG	1 cut(s)	1092
PauI	GCGCGC	1 cut(s)	194
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PctI	GAATGC	1 cut(s)	1302
PdiI	GCCGGC	1 cut(s)	986
PfeI	GAWTC	1 cut(s)	245
Ppu21I	YACGTR	1 cut(s)	630
Psp124BI	GAGCTC	1 cut(s)	670
Psp6I	CCWGG	1 cut(s)	1336
PspGI	CCWGG	1 cut(s)	1336
PspN4I	GGNNCC	1 cut(s)	615
PstI	CTGCAG	2 cut(s)	240, 480
PteI	GCGCGC	1 cut(s)	194
PvuII	CAGCTG	1 cut(s)	1347
RsaI	GTAC	4 cut(s)	389, 458, 655, 725
RsaNI	GTAC	4 cut(s)	388, 457, 654, 724
SacI	GAGCTC	1 cut(s)	670
SaqAI	TTAA	3 cut(s)	777, 1452, 1492
Sau3AI	GATC	5 cut(s)	280, 883, 1005, 1036, 1423
ScrFI	CCNGG	2 cut(s)	847, 1338
SduI	GDGCHC	4 cut(s)	670, 691, 948, 1145
SfaNI	GCATC	4 cut(s)	455, 772, 913, 1101
SfcI	CTRYAG	3 cut(s)	236, 476, 1159
Sfr274I	CTCGAG	1 cut(s)	1092
SfuI	TTCGAA	1 cut(s)	798
SlaI	CTCGAG	1 cut(s)	1092
SmlI	CTYRAG	1 cut(s)	1092
SmoI	CTYRAG	1 cut(s)	1092
SnaBI	TACGTA	1 cut(s)	630
Sse9I	AATT	9 cut(s)	107, 408, 718, 802, 997, 1097, 1247, 1376, 1416
SsiI	CCGC	9 cut(s)	189, 192, 199, 202, 378, 468, 904, 1069, 1199
SspMI	CTAG	3 cut(s)	1034, 1217, 1266
SstI	GAGCTC	1 cut(s)	670
StyD4I	CCNGG	2 cut(s)	845, 1336
TaaI	ACNGT	3 cut(s)	262, 395, 1394
TaiI	ACGT	2 cut(s)	632, 913
TaqI	TCGA	6 cut(s)	162, 318, 364, 498, 798, 1093
TasI	AATT	9 cut(s)	107, 408, 718, 802, 997, 1097, 1247, 1376, 1416
TatI	WGTACW	2 cut(s)	456, 653
TauI	GCSGC	5 cut(s)	194, 201, 204, 907, 1071
TfiI	GAWTC	1 cut(s)	245
Tru1I	TTAA	3 cut(s)	777, 1452, 1492
Tru9I	TTAA	3 cut(s)	777, 1452, 1492
TscAI	CASTG	3 cut(s)	398, 598, 667
TseFI	GTSAC	1 cut(s)	333
TseI	GCWGC	7 cut(s)	398, 475, 583, 856, 1147, 1196, 1289
Tsp45I	GTSAC	1 cut(s)	333
TspDTI	ATGAA	9 cut(s)	17, 63, 336, 362, 914, 1013, 1090, 1325, 1380
TspGWI	ACGGA	1 cut(s)	46
TspRI	CASTG	3 cut(s)	398, 598, 667
XapI	RAATTY	4 cut(s)	107, 802, 1097, 1376
XcmI	CCANNNNNNNNNTGG	1 cut(s)	934
XhoI	CTCGAG	1 cut(s)	1092
XspI	CTAG	3 cut(s)	1034, 1217, 1266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.