FvH4_6g22570

Protein CWC15 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006245
Physical Location & Seq
Forward (+)
16234434 .. 16237079
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22570.t1

Sequence Viewer

Length: 696 bp
ATGACGACTGCAGCTCGACCCACATGGGCTCCTGCAAAAGGTGGTAATGAACAAGGTGGTACTCGGATTTTTGGTCCATCCCAGAAGTACTCATCAAGGGACATTGCGTCGCATACAACTCTAAAACCCAGGAGGGATGGCCAGGACACCCAGGAAGAACTGCACAAGAGGAACCTCCAGGAGGAGCTGGAAGACCGTGAGAGGAGGCATTTTTCATCGAAGGATAAATATAGTTATGACAGAGATCGCAGCAAGAGAGGAAGCCAGCTTTTGTTGGAAGGGACAAGAAGGGACACTGAAGACCGCATTGTACCACGTAATGTAGATGCTGATGATTCTGATTTGGATGTCAAAAGTGATGATGACAGTGATGAAGATGATGATGATGATGAGGATGATACAGAAGCTCTTTTGGCTGAGCTCGAACAAATAAAGAGGGAAAGAGCAGAGGAGAAGAAGCGAAAGGAAGAACTACAGTTGGCAGAAGAGCTTAAAGTCAAGGAAGCAGAGCTTCTCAAAGGAAACCCATTGCTTAATAATCCAATAGCCGCTTCTTTCAGTGTGAAAAGAAGATGGGATGATGATGTGGTATTCAAAAATCAGGCCCGTGGTGAAACCAAAACTCCCAAGCGCTTCATCAATGACACAGTGAGGAATGACTTCCACCGCAAGTTTCTGCATAAATACATGAAGTAA

Protein Analysis

232

Amino Acids

26.98

Weight (kDa)

5.47

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cwf_Cwc_15 PF04889 1 - 231 4.5e-76 Cwf15/Cwc15 cell cycle control protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 304, 549, 667
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 3 cut(s) 61, 89, 312
AfeI AGCGCT 1 cut(s) 632
AfiI CCNNNNNNNGG 2 cut(s) 38, 181
AgsI TTSAA 1 cut(s) 595
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 4 cut(s) 128, 141, 150, 177
AjuI GAANNNNNNNTTGG 2 cut(s) 535, 567
AluBI AGCT 7 cut(s) 14, 187, 268, 407, 421, 490, 511
AluI AGCT 7 cut(s) 14, 187, 268, 407, 421, 490, 511
Alw21I GWGCWC 1 cut(s) 423
Aor51HI AGCGCT 1 cut(s) 632
AoxI GGCC 2 cut(s) 139, 603
ApeKI GCWGC 2 cut(s) 11, 249
Asp700I GAANNNNTTC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 633
AspS9I GGNCC 2 cut(s) 74, 604
AsuHPI GGTGA 1 cut(s) 623
AvaII GGWCC 1 cut(s) 74
BalI TGGCCA 1 cut(s) 141
BanII GRGCYC 2 cut(s) 31, 423
BbsI GAAGAC 2 cut(s) 198, 306
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 2 cut(s) 23, 261
BccI CCATC 3 cut(s) 85, 131, 567
BciT130I CCWGG 4 cut(s) 130, 143, 152, 179
BfmI CTRYAG 2 cut(s) 9, 473
BfoI RGCGCY 1 cut(s) 634
BisI GCNGC 3 cut(s) 12, 250, 549
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 3 cut(s) 13, 251, 550
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 4 cut(s) 130, 143, 152, 179
Bme18I GGWCC 1 cut(s) 74
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 74, 604
BmiI GGNNCC 2 cut(s) 30, 173
BmrFI CCNGG 4 cut(s) 130, 143, 152, 179
BmsI GCATC 1 cut(s) 316
BpiI GAAGAC 2 cut(s) 198, 306
BpmI CTGGAG 1 cut(s) 161
Bpu1102I GCTNAGC 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 3 cut(s) 128, 150, 607
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 607, 637
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 181
Bse3DI GCAATG 2 cut(s) 102, 527
BseBI CCWGG 4 cut(s) 130, 143, 152, 179
BseDI CCNNGG 3 cut(s) 128, 150, 607
BseGI GGATG 5 cut(s) 77, 142, 352, 400, 583
BseLI CCNNNNNNNGG 2 cut(s) 38, 181
BseMI GCAATG 2 cut(s) 102, 527
BseMII CTCAG 1 cut(s) 408
BseRI GAGGAG 3 cut(s) 197, 217, 464
BseXI GCAGC 2 cut(s) 23, 261
BsgI GTGCAG 1 cut(s) 146
BshFI GGCC 2 cut(s) 141, 605
BsiHKAI GWGCWC 1 cut(s) 423
BslFI GGGAC 3 cut(s) 113, 295, 305
BslI CCNNNNNNNGG 2 cut(s) 38, 181
BsmFI GGGAC 3 cut(s) 113, 295, 305
BsnI GGCC 2 cut(s) 141, 605
Bsp1286I GDGCHC 2 cut(s) 31, 423
Bsp143I GATC 1 cut(s) 244
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 3 cut(s) 304, 549, 667
BspANI GGCC 2 cut(s) 141, 605
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 30, 173
BspMAI CTGCAG 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 480
BsrDI GCAATG 2 cut(s) 102, 527
BssECI CCNNGG 3 cut(s) 128, 150, 607
BssMI GATC 1 cut(s) 244
Bst2UI CCWGG 4 cut(s) 130, 143, 152, 179
Bst4CI ACNGT 4 cut(s) 197, 368, 477, 649
Bst6I CTCTTC 1 cut(s) 480
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 417
BstDSI CCRYGG 1 cut(s) 607
BstENI CCTNNNNNAGG 2 cut(s) 36, 179
BstF5I GGATG 5 cut(s) 77, 142, 352, 400, 583
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstNI CCWGG 4 cut(s) 130, 143, 152, 179
BstSCI CCNGG 4 cut(s) 128, 141, 150, 177
BstSFI CTRYAG 2 cut(s) 9, 473
BstV1I GCAGC 2 cut(s) 23, 261
BstV2I GAAGAC 2 cut(s) 198, 306
BsuRI GGCC 2 cut(s) 141, 605
BtgI CCRYGG 1 cut(s) 607
BtsCI GGATG 5 cut(s) 77, 142, 352, 400, 583
BtsIMutI CAGTG 4 cut(s) 294, 373, 565, 654
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 1 cut(s) 633
Cfr13I GGNCC 2 cut(s) 74, 604
CseI GACGC 1 cut(s) 96
Csp6I GTAC 3 cut(s) 60, 88, 311
CviAII CATG 2 cut(s) 24, 688
CviQI GTAC 3 cut(s) 60, 88, 311
DdeI CTNAG 1 cut(s) 417
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
DriI GACNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 480
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 421
Eco24I GRGCYC 2 cut(s) 31, 423
Eco47I GGWCC 1 cut(s) 74
Eco47III AGCGCT 1 cut(s) 632
Eco53kI GAGCTC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 421
EcoNI CCTNNNNNAGG 2 cut(s) 36, 179
EcoRII CCWGG 4 cut(s) 128, 141, 150, 177
EcoT38I GRGCYC 2 cut(s) 31, 423
FaeI CATG 2 cut(s) 27, 691
FaiI YATR 6 cut(s) 25, 114, 231, 237, 681, 689
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 3 cut(s) 113, 295, 305
FatI CATG 2 cut(s) 23, 687
Fnu4HI GCNGC 3 cut(s) 12, 250, 549
FokI GGATG 5 cut(s) 64, 149, 359, 407, 590
FriOI GRGCYC 2 cut(s) 31, 423
Fsp4HI GCNGC 3 cut(s) 12, 250, 549
GlaI GCGC 1 cut(s) 632
GluI GCNGC 3 cut(s) 12, 250, 549
GsuI CTGGAG 1 cut(s) 161
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 2 cut(s) 141, 605
HgaI GACGC 1 cut(s) 96
HhaI GCGC 1 cut(s) 633
Hin1II CATG 2 cut(s) 27, 691
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HinfI GANTC 1 cut(s) 335
HphI GGTGA 1 cut(s) 623
Hpy188I TCNGA 2 cut(s) 66, 340
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 3 cut(s) 214, 272, 282
HpyCH4III ACNGT 4 cut(s) 197, 368, 477, 649
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 4 cut(s) 11, 35, 163, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 2 cut(s) 27, 691
HspAI GCGC 1 cut(s) 631
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 2 cut(s) 34, 184
Lsp1109I GCAGC 2 cut(s) 23, 261
LweI GCATC 1 cut(s) 316
MaeII ACGT 1 cut(s) 316
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 8 cut(s) 167, 203, 311, 386, 466, 479, 497, 582
MhlI GDGCHC 2 cut(s) 31, 423
MlsI TGGCCA 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 255
Mox20I TGGCCA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 659
MscI TGGCCA 1 cut(s) 141
MseI TTAA 2 cut(s) 492, 534
Msp20I TGGCCA 1 cut(s) 141
MspR9I CCNGG 4 cut(s) 130, 143, 152, 179
MvaI CCWGG 4 cut(s) 130, 143, 152, 179
MwoI GCNNNNNNNGC 1 cut(s) 413
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 27, 691
NlaIV GGNNCC 2 cut(s) 30, 173
PciSI GCTCTTC 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 3 cut(s) 13, 251, 550
Ppu21I YACGTR 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 423
Psp6I CCWGG 4 cut(s) 128, 141, 150, 177
PspGI CCWGG 4 cut(s) 128, 141, 150, 177
PspN4I GGNNCC 2 cut(s) 30, 173
PspPI GGNCC 2 cut(s) 74, 604
PstI CTGCAG 1 cut(s) 13
RsaI GTAC 3 cut(s) 61, 89, 312
RsaNI GTAC 3 cut(s) 60, 88, 311
SacI GAGCTC 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 2 cut(s) 492, 534
SatI GCNGC 3 cut(s) 12, 250, 549
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 74, 604
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 4 cut(s) 130, 143, 152, 179
SduI GDGCHC 2 cut(s) 31, 423
SfaNI GCATC 1 cut(s) 316
SfcI CTRYAG 2 cut(s) 9, 473
SinI GGWCC 1 cut(s) 74
SsiI CCGC 3 cut(s) 304, 549, 667
SstI GAGCTC 1 cut(s) 423
StyD4I CCNGG 4 cut(s) 128, 141, 150, 177
TaaI ACNGT 4 cut(s) 197, 368, 477, 649
TaiI ACGT 1 cut(s) 319
TaqI TCGA 3 cut(s) 16, 218, 423
TatI WGTACW 1 cut(s) 87
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 2 cut(s) 492, 534
Tru9I TTAA 2 cut(s) 492, 534
TscAI CASTG 4 cut(s) 301, 373, 565, 654
TseI GCWGC 2 cut(s) 11, 249
TspDTI ATGAA 4 cut(s) 63, 204, 387, 625
TspRI CASTG 4 cut(s) 301, 373, 565, 654
VpaK11BI GGWCC 1 cut(s) 74
XagI CCTNNNNNAGG 2 cut(s) 36, 179
XmnI GAANNNNTTC 1 cut(s) 659
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.