Rw1G004300

Protein CWC15 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006245
Physical Location & Seq
Reverse (-)
8158561 .. 8161301
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004300.1

Sequence Viewer

Length: 675 bp
ATGACGACTGCAGCTCGACCCACGAGGGCTCCTGCGAAAGGTGGTAATGAACAATGTGGTACTCAGATTTTTGGCCCATCCCAAAAGTACTCATCAAGGGACATTGCATCCCATACAACTCTAAAACCCAAAAAGGACGGAAAGGACATCCAGGAAGAACTGCAGAAAAGGAACCTCAAGGAGGAGTTGGAAGACCATGACAGGAGGCATTTTTCATCCAAGGATAAATATAGTTATGACAGAGATCGCAGCAGGAGAGGGAGCCAGCTTTTGTTGGAAAGGATAAGAAGGGACACTGAAGACTGCGTTATACCACGTAATGTAGATGCTGATGATTTTGATATGGAAGTCAAAAGTGATGATGACAGACACCTTGAAGATAATCTGGATGATTGGCTTGCAGAGGGATTGGAGGATTACCTGGATGATGACTACTTAGTTTTTGACTGCCCAGATCATATTGAAGGATTTCAAAATTTCTCCAAAACCCCCTTTCTCAACTCACAAATATTGATGAAAAAGACACATATGAGTAATTTTTTTGTGTCCTCTCAAGGATTTAAGGACACCATTTCTAGATCTTTTAGGACACAGTTTGTGTGTCCTAGTATAGAAAAGAGGACACAGTTTCTACAGCTTTTAGGACATTGTTTAGGTGTCCTCTTATGGATTTAA

Protein Analysis

224

Amino Acids

26.12

Weight (kDa)

5.54

Isoelectric Point (pI)

54.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cwf_Cwc_15 PF04889 1 - 126 2.1e-19 Cwf15/Cwc15 cell cycle control protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 2 cut(s) 61, 89
AfiI CCNNNNNNNGG 2 cut(s) 38, 181
AgsI TTSAA 3 cut(s) 377, 464, 473
AjnI CCWGG 2 cut(s) 150, 420
AluBI AGCT 3 cut(s) 14, 268, 637
AluI AGCT 3 cut(s) 14, 268, 637
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 11, 249
ApoI RAATTY 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 468
AspS9I GGNCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 31
BauI CACGAG 1 cut(s) 22
BbsI GAAGAC 2 cut(s) 198, 306
BbvI GCAGC 2 cut(s) 23, 261
BccI CCATC 1 cut(s) 85
BciT130I CCWGG 2 cut(s) 152, 422
BfaI CTAG 2 cut(s) 576, 606
BfmI CTRYAG 3 cut(s) 9, 161, 632
BglII AGATCT 1 cut(s) 578
BisI GCNGC 2 cut(s) 12, 250
BlsI GCNGC 2 cut(s) 13, 251
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 152, 422
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 3 cut(s) 30, 173, 263
BmrFI CCNGG 2 cut(s) 152, 422
BmsI GCATC 2 cut(s) 116, 316
BpiI GAAGAC 2 cut(s) 198, 306
BpuEI CTTGAG 2 cut(s) 161, 537
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 181
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 2 cut(s) 152, 422
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 6 cut(s) 77, 107, 147, 215, 394, 430
BseLI CCNNNNNNNGG 2 cut(s) 38, 181
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 23, 261
BshFI GGCC 1 cut(s) 75
BslFI GGGAC 2 cut(s) 113, 305
BslI CCNNNNNNNGG 2 cut(s) 38, 181
BsmFI GGGAC 2 cut(s) 113, 305
BsnI GGCC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 3 cut(s) 244, 454, 578
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 30, 173, 263
BspMAI CTGCAG 2 cut(s) 13, 165
BsrDI GCAATG 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 3 cut(s) 244, 454, 578
BssSI CACGAG 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 22
Bst2UI CCWGG 2 cut(s) 152, 422
Bst4CI ACNGT 2 cut(s) 594, 627
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 266, 399
BstDEI CTNAG 2 cut(s) 63, 436
BstENI CCTNNNNNAGG 2 cut(s) 36, 179
BstF5I GGATG 6 cut(s) 77, 107, 147, 215, 394, 430
BstKTI GATC 3 cut(s) 247, 457, 581
BstMBI GATC 3 cut(s) 244, 454, 578
BstNI CCWGG 2 cut(s) 152, 422
BstSCI CCNGG 2 cut(s) 150, 420
BstSFI CTRYAG 3 cut(s) 9, 161, 632
BstV1I GCAGC 2 cut(s) 23, 261
BstV2I GAAGAC 2 cut(s) 198, 306
BstX2I RGATCY 1 cut(s) 578
BstYI RGATCY 1 cut(s) 578
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 6 cut(s) 77, 107, 147, 215, 394, 430
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 266, 399
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 60, 88
CviAII CATG 1 cut(s) 197
CviJI RGCY 7 cut(s) 14, 29, 75, 264, 268, 397, 637
CviKI_1 RGCY 7 cut(s) 14, 29, 75, 264, 268, 397, 637
CviQI GTAC 2 cut(s) 60, 88
DdeI CTNAG 2 cut(s) 63, 436
DpnI GATC 3 cut(s) 246, 456, 580
DpnII GATC 3 cut(s) 244, 454, 578
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 318
EcoNI CCTNNNNNAGG 2 cut(s) 36, 179
EcoRII CCWGG 2 cut(s) 150, 420
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 1 cut(s) 200
FaqI GGGAC 2 cut(s) 113, 305
FatI CATG 1 cut(s) 196
FauNDI CATATG 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 12, 250
FokI GGATG 6 cut(s) 64, 94, 134, 202, 401, 437
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 12, 250
FspBI CTAG 2 cut(s) 576, 606
GluI GCNGC 2 cut(s) 12, 250
HaeIII GGCC 1 cut(s) 75
Hin1II CATG 1 cut(s) 200
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 2 cut(s) 386, 576
HpyAV CCTTC 2 cut(s) 282, 458
HpyCH4III ACNGT 2 cut(s) 594, 627
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 4 cut(s) 11, 107, 163, 401
HpyF3I CTNAG 2 cut(s) 63, 436
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 1 cut(s) 200
Kzo9I GATC 3 cut(s) 244, 454, 578
LmnI GCTCC 2 cut(s) 34, 261
Lsp1109I GCAGC 2 cut(s) 23, 261
LweI GCATC 2 cut(s) 116, 316
MaeI CTAG 2 cut(s) 576, 606
MaeII ACGT 1 cut(s) 316
MalI GATC 3 cut(s) 246, 456, 580
MboI GATC 3 cut(s) 244, 454, 578
MboII GAAGA 4 cut(s) 167, 203, 311, 389
MflI RGATCY 1 cut(s) 578
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 2 cut(s) 475, 535
MmeI TCCRAC 2 cut(s) 168, 255
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 2 cut(s) 561, 673
MspR9I CCNGG 2 cut(s) 152, 422
MvaI CCWGG 2 cut(s) 152, 422
NdeI CATATG 1 cut(s) 528
NdeII GATC 3 cut(s) 244, 454, 578
NlaIII CATG 1 cut(s) 200
NlaIV GGNNCC 3 cut(s) 30, 173, 263
PdmI GAANNNNTTC 1 cut(s) 468
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 2 cut(s) 13, 251
Ppu21I YACGTR 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 150, 420
PspGI CCWGG 2 cut(s) 150, 420
PspN4I GGNNCC 3 cut(s) 30, 173, 263
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 2 cut(s) 13, 165
PsuI RGATCY 1 cut(s) 578
RsaI GTAC 2 cut(s) 61, 89
RsaNI GTAC 2 cut(s) 60, 88
SaqAI TTAA 2 cut(s) 561, 673
SatI GCNGC 2 cut(s) 12, 250
Sau3AI GATC 3 cut(s) 244, 454, 578
Sau96I GGNCC 1 cut(s) 74
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 152, 422
SduI GDGCHC 1 cut(s) 31
SetI ASST 9 cut(s) 16, 43, 177, 270, 319, 375, 423, 639, 658
SfaNI GCATC 2 cut(s) 116, 316
SfcI CTRYAG 3 cut(s) 9, 161, 632
SmlI CTYRAG 2 cut(s) 176, 552
SmoI CTYRAG 2 cut(s) 176, 552
Sse9I AATT 2 cut(s) 475, 535
SspI AATATT 1 cut(s) 510
SspMI CTAG 2 cut(s) 576, 606
StyD4I CCNGG 2 cut(s) 150, 420
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 594, 627
TaiI ACGT 1 cut(s) 319
TaqI TCGA 1 cut(s) 16
TasI AATT 2 cut(s) 475, 535
TatI WGTACW 1 cut(s) 87
Tru1I TTAA 2 cut(s) 561, 673
Tru9I TTAA 2 cut(s) 561, 673
TscAI CASTG 1 cut(s) 301
TseI GCWGC 2 cut(s) 11, 249
TspDTI ATGAA 3 cut(s) 63, 204, 530
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 301
XagI CCTNNNNNAGG 2 cut(s) 36, 179
XapI RAATTY 1 cut(s) 475
XbaI TCTAGA 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 2 cut(s) 576, 606
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.