Rw7G024110

Protein CWC15 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006245
Physical Location & Seq
Reverse (-)
29424357 .. 29426539
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G024110.1

Sequence Viewer

Length: 519 bp
ATGACGACTGCAGCTCGACCCACGAGGGCTCCTGCGAAAGCTGGTAATGAACAAGGTGGTACTCAGATTTTTGGCCCATCCCAAAAGTACTCATCAAGGGACATTGCATCCCATACAACTCTAAAACCCAAAAAGGACGGAAAGGACATCCAGGAAGAACTGCAGAAAAGGAACCTCCAGGAGGAGTTGGAAGACCATGAGAGGAGGCATTTTTCATCAAAGGATAAATATAGTTATGACAGAGATCGCAGCAGGAGAGGGAGCCAACTTTTGTTGGAAAGGATAAGAAGGGACACTGAAGACTGCGTTATACCACGTAATGTAGATGCTGATGATTCTGATATGGAAGTCAAAAGTGATGATGACAGACACCTTGAAGATAATCTGGATGATTGGCTTGCAGAGGGATTGGAGGATTACTTGGATGATGACTACTTAGTTAAGTATGCAATGCTAGTTGTTACATTATCCATGCATACCAATTGTAACAGGAGACTACTAATGTGTTATCTCAAATAG

Protein Analysis

172

Amino Acids

20.07

Weight (kDa)

5.3

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cwf_Cwc_15 PF04889 1 - 127 8.5e-20 Cwf15/Cwc15 cell cycle control protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 2 cut(s) 61, 89
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 1 cut(s) 377
AjnI CCWGG 2 cut(s) 150, 177
AluBI AGCT 2 cut(s) 14, 41
AluI AGCT 2 cut(s) 14, 41
Alw26I GTCTC 1 cut(s) 487
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 11, 249
AspS9I GGNCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 31
BauI CACGAG 1 cut(s) 22
BbsI GAAGAC 2 cut(s) 198, 306
BbvI GCAGC 2 cut(s) 23, 261
BccI CCATC 1 cut(s) 85
BciT130I CCWGG 2 cut(s) 152, 179
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 2 cut(s) 9, 161
BisI GCNGC 2 cut(s) 12, 250
BlsI GCNGC 2 cut(s) 13, 251
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 152, 179
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 3 cut(s) 30, 173, 263
BmrFI CCNGG 2 cut(s) 152, 179
BmsI GCATC 2 cut(s) 116, 316
BpiI GAAGAC 2 cut(s) 198, 306
BpmI CTGGAG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse3DI GCAATG 2 cut(s) 102, 456
BseBI CCWGG 2 cut(s) 152, 179
BseGI GGATG 5 cut(s) 77, 107, 147, 394, 430
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 2 cut(s) 102, 456
BseMII CTCAG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 197, 217
BseXI GCAGC 2 cut(s) 23, 261
BshFI GGCC 1 cut(s) 75
BslFI GGGAC 2 cut(s) 113, 305
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 487
BsmFI GGGAC 2 cut(s) 113, 305
BsnI GGCC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 244
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 30, 173, 263
BspMAI CTGCAG 2 cut(s) 13, 165
BsrDI GCAATG 2 cut(s) 102, 456
BssMI GATC 1 cut(s) 244
BssSI CACGAG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 22
Bst2UI CCWGG 2 cut(s) 152, 179
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 63, 436
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 5 cut(s) 77, 107, 147, 394, 430
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 1 cut(s) 244
BstNI CCWGG 2 cut(s) 152, 179
BstSCI CCNGG 2 cut(s) 150, 177
BstSFI CTRYAG 2 cut(s) 9, 161
BstV1I GCAGC 2 cut(s) 23, 261
BstV2I GAAGAC 2 cut(s) 198, 306
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 5 cut(s) 77, 107, 147, 394, 430
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 60, 88
CviAII CATG 2 cut(s) 197, 472
CviJI RGCY 6 cut(s) 14, 29, 41, 75, 264, 397
CviKI_1 RGCY 6 cut(s) 14, 29, 41, 75, 264, 397
CviQI GTAC 2 cut(s) 60, 88
DdeI CTNAG 2 cut(s) 63, 436
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 150, 177
EcoT22I ATGCAT 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 31
FaeI CATG 2 cut(s) 200, 475
FaiI YATR 9 cut(s) 114, 198, 231, 237, 311, 344, 447, 473, 477
FaqI GGGAC 2 cut(s) 113, 305
FatI CATG 2 cut(s) 196, 471
Fnu4HI GCNGC 2 cut(s) 12, 250
FokI GGATG 5 cut(s) 64, 94, 134, 401, 437
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 12, 250
FspBI CTAG 1 cut(s) 455
GluI GCNGC 2 cut(s) 12, 250
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 1 cut(s) 75
Hin1II CATG 2 cut(s) 200, 475
HinfI GANTC 1 cut(s) 335
Hpy188I TCNGA 2 cut(s) 66, 340
Hpy188III TCNNGA 1 cut(s) 386
HpyAV CCTTC 1 cut(s) 282
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 6 cut(s) 11, 107, 163, 401, 449, 475
HpyF3I CTNAG 2 cut(s) 63, 436
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 2 cut(s) 200, 475
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 2 cut(s) 34, 261
LpnPI CCDG 9 cut(s) 27, 45, 137, 164, 164, 191, 238, 371, 475
Lsp1109I GCAGC 2 cut(s) 23, 261
LweI GCATC 2 cut(s) 116, 316
MaeI CTAG 1 cut(s) 455
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 2 cut(s) 460, 485
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 4 cut(s) 167, 203, 311, 389
MfeI CAATTG 1 cut(s) 481
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 1 cut(s) 481
MmeI TCCRAC 2 cut(s) 168, 255
MnlI CCTC 8 cut(s) 18, 175, 185, 195, 198, 251, 397, 406
Mph1103I ATGCAT 1 cut(s) 477
MseI TTAA 1 cut(s) 441
MspR9I CCNGG 2 cut(s) 152, 179
MunI CAATTG 1 cut(s) 481
MvaI CCWGG 2 cut(s) 152, 179
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 200, 475
NlaIV GGNNCC 3 cut(s) 30, 173, 263
NsiI ATGCAT 1 cut(s) 477
PfeI GAWTC 1 cut(s) 335
PfoI TCCNGGA 2 cut(s) 150, 177
PkrI GCNGC 2 cut(s) 13, 251
Ppu21I YACGTR 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 150, 177
PspGI CCWGG 2 cut(s) 150, 177
PspN4I GGNNCC 3 cut(s) 30, 173, 263
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 2 cut(s) 13, 165
RsaI GTAC 2 cut(s) 61, 89
RsaNI GTAC 2 cut(s) 60, 88
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 2 cut(s) 12, 250
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 74
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 152, 179
SduI GDGCHC 1 cut(s) 31
SetI ASST 6 cut(s) 16, 43, 58, 177, 319, 375
SfaNI GCATC 2 cut(s) 116, 316
SfcI CTRYAG 2 cut(s) 9, 161
Sse9I AATT 1 cut(s) 481
SspMI CTAG 1 cut(s) 455
StyD4I CCNGG 2 cut(s) 150, 177
TaiI ACGT 1 cut(s) 319
TaqI TCGA 1 cut(s) 16
TasI AATT 1 cut(s) 481
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 1 cut(s) 301
TseI GCWGC 2 cut(s) 11, 249
TspDTI ATGAA 2 cut(s) 63, 204
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 301
XagI CCTNNNNNAGG 1 cut(s) 179
XspI CTAG 1 cut(s) 455
ZrmI AGTACT 1 cut(s) 89
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.