MD03G1032500.v1.1

Protein CWC15 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006245
Physical Location & Seq
Reverse (-)
2615407 .. 2618300
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1032500.v1.1.491

Sequence Viewer

Length: 690 bp
ATGACGACGGCCGCTAGACCCACTTGGGCACCTGCGAAAGGTGGCAATGAACAAGGTGGTACTCGGATTTTTGGCCCATCACAGAAATACTCATCAAGGGACATGGCTGCCCATACATCTCTAAAACCCAGAAAGCCGGGGCAGGACACCCAGGATGAGTTGCAGAATAAAAACCTCCGGGAGGAACTGGAAGAGAGCGAAAGGAAGCATTATTCATCAAAGGATAAATCTTATAGTTATGACAGAGATCGCAGGAGGGGGAACCAGCTGTTATTGGAAGGGGGCAGAAGGGACACTGAAATTGTGCCACGTAATATAGATGCTGATGATTCTGATGTGGAAGCCCAAAGTGATGATGAAAGTGGTGAAGATGACGATGACGAGGAGGATGACATGGAAGCTCTATTGGCCGAGCTTGAACAAATAAAGAAGGAAAAAGCAGAGGAGAAGTTACGGAAGGAAAGACTAGAGAGGGAGGAAGAGCTTAAAGTCAAGGAAGCAGAGCTCCTCCGAGGAAACCCATTGCTTAATGGTGCAGCACCCGCATCATTTAGTGTGAAAAGAAGATGGGATGACGATGTGGTGTTCAAGAATCAGGCCCGCGGGGAAACCAAGACTCCTAAGCGCTTCATCAATGACACAGTCAGGAGTGACTTTCACCGCAAATTCCTGCAGAAATACATGAAGTAA

Protein Analysis

230

Amino Acids

26.49

Weight (kDa)

5.32

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cwf_Cwc_15 PF04889 1 - 229 7.8e-76 Cwf15/Cwc15 cell cycle control protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 28
AccII CGCG 1 cut(s) 603
AciI CCGC 5 cut(s) 12, 543, 601, 603, 661
AcoI YGGCCR 2 cut(s) 9, 408
AcsI RAATTY 1 cut(s) 665
AfaI GTAC 1 cut(s) 61
AfeI AGCGCT 1 cut(s) 626
AfiI CCNNNNNNNGG 2 cut(s) 38, 181
AgsI TTSAA 2 cut(s) 419, 589
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 5 cut(s) 268, 401, 415, 484, 505
AluI AGCT 5 cut(s) 268, 401, 415, 484, 505
Alw21I GWGCWC 1 cut(s) 507
Aor51HI AGCGCT 1 cut(s) 626
AoxI GGCC 4 cut(s) 9, 73, 408, 597
ApeKI GCWGC 2 cut(s) 107, 536
ApoI RAATTY 1 cut(s) 665
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 2 cut(s) 74, 598
AsuC2I CCSGG 2 cut(s) 138, 179
AsuHPI GGTGA 2 cut(s) 377, 650
BaeGI GKGCMC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 94, 548
BccI CCATC 2 cut(s) 85, 561
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 1 cut(s) 152
BcnI CCSGG 2 cut(s) 138, 179
BfaI CTAG 2 cut(s) 15, 467
BfmI CTRYAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 3 cut(s) 12, 108, 537
BlsI GCNGC 3 cut(s) 13, 109, 538
Bme1390I CCNGG 3 cut(s) 138, 152, 179
BmgT120I GGNCC 2 cut(s) 74, 598
BmiI GGNNCC 2 cut(s) 30, 263
BmrFI CCNGG 3 cut(s) 138, 152, 179
BmsI GCATC 2 cut(s) 310, 554
Bpu10I CCTNAGC 1 cut(s) 621
BpuMI CCSGG 2 cut(s) 138, 179
BsaAI YACGTR 1 cut(s) 311
BsaJI CCNNGG 4 cut(s) 137, 150, 511, 601
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 181
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 2 cut(s) 52, 521
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 4 cut(s) 137, 150, 511, 601
BseGI GGATG 3 cut(s) 160, 394, 577
BseLI CCNNNNNNNGG 2 cut(s) 38, 181
BseMI GCAATG 2 cut(s) 52, 521
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 3 cut(s) 398, 458, 497
BseSI GKGCMC 1 cut(s) 31
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 2 cut(s) 94, 548
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 603
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 11, 75, 410, 599
BshNI GGYRCC 1 cut(s) 28
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 507
BsiSI CCGG 2 cut(s) 137, 178
BslFI GGGAC 2 cut(s) 113, 305
BslI CCNNNNNNNGG 2 cut(s) 38, 181
BsmFI GGGAC 2 cut(s) 113, 305
BsnI GGCC 4 cut(s) 11, 75, 410, 599
Bsp1286I GDGCHC 2 cut(s) 31, 507
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 5 cut(s) 12, 543, 601, 603, 661
BspANI GGCC 4 cut(s) 11, 75, 410, 599
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 2 cut(s) 30, 263
BspMAI CTGCAG 1 cut(s) 675
BspMI ACCTGC 1 cut(s) 40
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 2 cut(s) 52, 521
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 4 cut(s) 137, 150, 511, 601
BssMI GATC 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 643
Bst6I CTCTTC 2 cut(s) 186, 474
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 601
BstDEI CTNAG 1 cut(s) 621
BstDSI CCRYGG 1 cut(s) 601
BstENI CCTNNNNNAGG 2 cut(s) 36, 179
BstF5I GGATG 3 cut(s) 160, 394, 577
BstFNI CGCG 1 cut(s) 603
BstH2I RGCGCY 1 cut(s) 628
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 1 cut(s) 250
BstMBI GATC 1 cut(s) 247
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 407, 542
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 3 cut(s) 136, 150, 177
BstSFI CTRYAG 1 cut(s) 671
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 2 cut(s) 94, 548
BstZI CGGCCG 1 cut(s) 9
BsuRI GGCC 4 cut(s) 11, 75, 410, 599
BtgI CCRYGG 1 cut(s) 601
BtsCI GGATG 3 cut(s) 160, 394, 577
BtsIMutI CAGTG 1 cut(s) 294
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 601
CfoI GCGC 1 cut(s) 627
Cfr13I GGNCC 2 cut(s) 74, 598
Cfr42I CCGCGG 1 cut(s) 604
Csp6I GTAC 1 cut(s) 60
CviAII CATG 3 cut(s) 103, 394, 682
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 1 cut(s) 621
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 9, 408
EagI CGGCCG 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 186, 474
EarI CTCTTC 2 cut(s) 186, 474
Ecl136II GAGCTC 1 cut(s) 505
EclXI CGGCCG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 507
Eco47III AGCGCT 1 cut(s) 626
Eco52I CGGCCG 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 505
EcoICRI GAGCTC 1 cut(s) 505
EcoNI CCTNNNNNAGG 2 cut(s) 36, 179
EcoRII CCWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 507
FaeI CATG 3 cut(s) 106, 397, 685
FaiI YATR 7 cut(s) 104, 114, 234, 240, 317, 395, 683
FaqI GGGAC 2 cut(s) 113, 305
FatI CATG 3 cut(s) 102, 393, 681
FauI CCCGC 3 cut(s) 550, 596, 608
Fnu4HI GCNGC 3 cut(s) 12, 108, 537
FokI GGATG 3 cut(s) 167, 401, 584
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 3 cut(s) 12, 108, 537
FspBI CTAG 2 cut(s) 15, 467
GlaI GCGC 1 cut(s) 626
GluI GCNGC 3 cut(s) 12, 108, 537
HaeII RGCGCY 1 cut(s) 628
HaeIII GGCC 4 cut(s) 11, 75, 410, 599
HapII CCGG 2 cut(s) 137, 178
HhaI GCGC 1 cut(s) 627
Hin1II CATG 3 cut(s) 106, 397, 685
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HinfI GANTC 3 cut(s) 329, 592, 616
HpaII CCGG 2 cut(s) 137, 178
HphI GGTGA 2 cut(s) 377, 650
Hpy188I TCNGA 3 cut(s) 66, 334, 512
Hpy188III TCNNGA 2 cut(s) 589, 646
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 272, 282, 424, 451
HpyCH4III ACNGT 1 cut(s) 643
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 3 cut(s) 163, 536, 673
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 542
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 3 cut(s) 106, 397, 685
HspAI GCGC 1 cut(s) 625
KspI CCGCGG 1 cut(s) 604
Kzo9I GATC 1 cut(s) 247
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 1 cut(s) 510
Lsp1109I GCAGC 2 cut(s) 94, 548
LweI GCATC 2 cut(s) 310, 554
MaeI CTAG 2 cut(s) 15, 467
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 450, 650
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 4 cut(s) 203, 380, 491, 576
MhlI GDGCHC 2 cut(s) 31, 507
MluCI AATT 2 cut(s) 300, 665
MlyI GAGTC 1 cut(s) 610
MseI TTAA 2 cut(s) 486, 528
MspA1I CMGCKG 2 cut(s) 268, 603
MspI CCGG 2 cut(s) 137, 178
MspR9I CCNGG 3 cut(s) 138, 152, 179
MvaI CCWGG 1 cut(s) 152
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 2 cut(s) 407, 542
NciI CCSGG 2 cut(s) 138, 179
NdeII GATC 1 cut(s) 247
NlaIII CATG 3 cut(s) 106, 397, 685
NlaIV GGNNCC 2 cut(s) 30, 263
NmeAIII GCCGAG 1 cut(s) 436
NmuCI GTSAC 1 cut(s) 650
PaqCI CACCTGC 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 474
PfeI GAWTC 2 cut(s) 329, 592
PflFI GACNNNGTC 1 cut(s) 641
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 3 cut(s) 13, 109, 538
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
Ppu21I YACGTR 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 30, 263
PspPI GGNCC 2 cut(s) 74, 598
PstI CTGCAG 1 cut(s) 675
PsyI GACNNNGTC 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SacI GAGCTC 1 cut(s) 507
SacII CCGCGG 1 cut(s) 604
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 2 cut(s) 486, 528
SatI GCNGC 3 cut(s) 12, 108, 537
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 2 cut(s) 74, 598
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 3 cut(s) 138, 152, 179
SduI GDGCHC 2 cut(s) 31, 507
SfaNI GCATC 2 cut(s) 310, 554
SfcI CTRYAG 1 cut(s) 671
Sfr303I CCGCGG 1 cut(s) 604
SgrBI CCGCGG 1 cut(s) 604
Sse9I AATT 2 cut(s) 300, 665
SsiI CCGC 5 cut(s) 12, 543, 601, 603, 661
SspMI CTAG 2 cut(s) 15, 467
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 3 cut(s) 136, 150, 177
TaaI ACNGT 1 cut(s) 643
TaiI ACGT 1 cut(s) 313
TasI AATT 2 cut(s) 300, 665
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 329, 592
Tru1I TTAA 2 cut(s) 486, 528
Tru9I TTAA 2 cut(s) 486, 528
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 2 cut(s) 107, 536
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 4 cut(s) 63, 204, 372, 619
TspGWI ACGGA 1 cut(s) 469
TspRI CASTG 1 cut(s) 301
Tth111I GACNNNGTC 1 cut(s) 641
XagI CCTNNNNNAGG 2 cut(s) 36, 179
XapI RAATTY 1 cut(s) 665
XspI CTAG 2 cut(s) 15, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.