Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0004898

Physical Location & Seq

Genomic Coordinates Forward (+)

26380112 .. 26389732

Gene Structure 9621 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g33350.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGGCAAAGAGTGAGCAGTGTTGCTCTACTCAGCTTATTGATGGAGACGGGTTTTTCAATGACACTGGAATCGAACAGTTTATAAAGGAAGTGAAATTGGGAGAATGTGGACTCTCCTATGCAGTAGTCTCCATCATGGGCCCACAAAGTAGTGGAAAGAGCACACTACTAAACAATTTGTTTGCTACCAACTTCAAGGAGATGGATGCTTTTAGAGGAAGGTCTCAAACAACAAAAGGTATTTGGTTGGCAAAATGTGCTGGTATTGAGCCTTGTACTCTTGTAATGGATTTGGAGGGTACAGATGGGAGAGAACGAGGAGAGGATGATACAGCATTCGAGAAGCAGAGTGCCCTCTTTGCTCTTGCCGTTTCCGATATAGTGCTCATCAACATGTGGTGCCATGATATTGGCCGTGAGCAAGCTGCAAATAAGCCTCTCCTAAAGACTGTGTTCCAGGTGATGATGCGATTGTTTAGTCCACGTAAAACAACTCTGATGTTTGTCATTCGTGATAAGACAAGGACGCCTTTGGAAAATTTAGAACCAGTTCTTAGAGAAGACATTCAGAAGATATGGGATTCAGTTCCGAAGCCTGAAGCTCACAAGGATACTCCACTAAGCGAATTTTTTAATGTCGAGGTTGTTGCTCTTTCAAGTTATGAAGAAAAAGAAGAGAAATTTAAAGAGCAGGTTGCTGGCTTGAGGCAGAAATTCTTCCATTCCATTGCCCCTGGTGGGCTTGCTGGAGACAGACGAGGGGTTGTTCCTGCTTCAGGGTTTTCTTTCAGTGCACAACAGATATGGAAAGTCATCAAAGAAAATAGAGATCTTGATCTTCCTGCCCACAAGGTCATGGTGGCAACTGTACGTTGTGAAGAGATTGCCAATGAGAAGCATGCTGCTTTTGTAGGAAATGAGGAGTGGAGTCAGTTGGAGCAGGACGTTCAATTGGGCCCCATTCCTGGCTTTGGAAAGAAGCTCAGTTCAATCATTGAAACGTCTCTTTCAGAGTATGATCAAGAAGCTACATATTTTGATGAAGGTGTCAGGTCTGGGAAGCGCAAGCAGCTTGAAGAAAAATTGCTTCAACTTGTCCAATCTGCATTCCAAGCTTTGCTGGGACACTTAAGATCTGGCACTCTGGAGAAGTTCAAGGTTGCATTTGATAAGGCCTTGGATGGCGGGGATGGTTTTTCTGCGGCTGCGCATAATTGTAGTGAATCTTTTATGGCACAATTTGATAAAGGATGTGCAGATGCTATTATTGAACAAGCAGACTGGGATGCATCTAAAGTAAGAGATAAACTTAAACGTGATATAGAAGCTCACATTGCTTCCGTTCGTGCTGCCAAGCTATCTGAAATCACTTCTCTTTATGAGGCAAAATTGAAGGAGGCCTTATCTGGACCGGTGGAAGCGCTGCTTGATGGAGCTAATAGTGAGACATGGCCTGCTATAAGGAAACTTTTCAAGCGCGAGACTGAATCAGCTGTCTCTGGGTTCTCTAGTGCACTTTCTAGTTTTGATATGGATAAACAAACCAAGGATAAGACTCTTGCAAGTCTAGAGGCATATGCAAGAGGCATAGTGGAAGCAAAAACAAAGGAAGAGGCTGGAAGGGTTTTGATCCGTATGAAGGACAGCCATGATTCTGATTCGATGCCACGTGTTTGGACTGGGAAGGAAGACATCAGAGCAATTACCAAGACTGCTCGTTCTGCATCCTTGAAGTTGCTGTCTGTAATGGCTGCAATTCGGTTGGATGATGGCGATACTGATAATATTGAGAAAACTTTATCCCTTGCCTTGGTGAATGCCAAAAATGCTGATGTCAAAGATAGGAGTATCACAACGGTTGACCCATTGGCCTCTAGCACTTGGCAAGAGGTTCCATCATCAAAGACATTAATCACACCTGTACAGTGCAAAAATTTGTGGAGGCAATTCAGGTCAGAGACGGAGTACAGTGTTTCTCAGGCTATTGCTGCCCAGGAAGCCAACAAGCGTAATAACAACTGGCTACCACCTCCGTGGGCTATTCTTGCCTTGGTTGTCCTCGGATTTAATGAATTCATGACACTCTTAAGAAATCCTCTGTATCTGCTGGTCATCTTTGTTGGCTTTCTACTCATTAAAGCACTATGGGTGCAGCTAGACATCGCGGCTGAATTCCGCAATGGCGCTCTTCCGGGACTTCTCTCTTTGTCCACCAAGTTGGTGCCAACTATCATGAACATGATGAAGAGATTAGCAGATGAAGGGGGAGCACCTTCTGCGTCTAACAATCCTCAAAGAAACCCTGCACCACCATCAAAAGGCTTAAGCAGTGGAGCAAATGCTAGCAGTTCGATGTCATCTACTGCGTCATCGGAAGTGACAGAATCTGAATACTCTAGTCCTACAAAACAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000902 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0006810 GO:0006888 GO:0006996 GO:0007010 GO:0007275 GO:0008150 GO:0009653 GO:0009826 GO:0009832 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0017076 GO:0019001 GO:0022622 GO:0030029 GO:0030036 GO:0030154 GO:0031090 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032989 GO:0035639 GO:0036094 GO:0040007 GO:0042546 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048364 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060560 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:0099402 GO:1901265 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RHD3

Protein Analysis

806

Amino Acids

88.73

Weight (kDa)

5.65

Isoelectric Point (pI)

43.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
URGCP_GTPase	PF25683	38 - 163	9.8e-08	URGCP-like GTPase domain
RHD3_GTPase	PF05879	46 - 281	7.3e-117	Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB	PF20428	323 - 667	5.6e-120	Sey1 three-helix bundle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13870 AT3G13870
fragaria_vesca	FvH4_6g33350 FvH4_6g33350 FvH4_6g33350 FvH4_6g33350
malus_domestica	MD09G1193400.v1.1 MD17G1174200.v1.1
prunus_persica	Prupe.3G049800_v2.0.a1
pyrus_communis	pycom09g10990 pycom17g17830
rosa_chinensis	RchiOBHm_Chr2g0143201 RchiOBHm_Chr2g0169871
rosa_laevigata	RLG00000020013
rosa_multiflora	Rmu_co8092052.1_g000001 Rmu_sc0000181.1_g000004 Rmu_sc0003950.1_g000012
rosa_roxburghii	Rroxscaffold_2G00102050 Rroxscaffold_5G00359600 Rroxscaffold_7G00190950 Rroxscaffold_7G00191100
rosa_rugosa	Rorug02G0379200
rosa_samantha	Rh1BG269400 Rh2AG432000 Rh2BG440600 Rh2CG385100 Rh2CG417900 Rh2DG450800 Rh7AG446900
rosa_wichuraiana	Rw2G035290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	83
Acc16I	TGCGCA	1 cut(s)	1209
Acc36I	ACCTGC	1 cut(s)	682
AccB1I	GGYRCC	2 cut(s)	399, 2221
AccII	CGCG	2 cut(s)	1479, 2165
AciI	CCGC	4 cut(s)	1185, 1202, 2165, 2176
AclWI	GGATC	1 cut(s)	1624
AcoI	YGGCCR	1 cut(s)	412
AcsI	RAATTY	7 cut(s)	538, 626, 680, 713, 1933, 2072, 2171
AcuI	CTGAAG	2 cut(s)	618, 759
AcvI	CACGTG	1 cut(s)	1670
AcyI	GRCGYC	1 cut(s)	527
AfaI	GTAC	5 cut(s)	277, 301, 870, 1923, 1967
AfeI	AGCGCT	1 cut(s)	1422
AfiI	CCNNNNNNNGG	7 cut(s)	738, 776, 965, 971, 1639, 1810, 2318
AflII	CTTAAG	3 cut(s)	1129, 2086, 2323
AflIII	ACRYGT	2 cut(s)	393, 1669
AgeI	ACCGGT	1 cut(s)	1411
AjnI	CCWGG	4 cut(s)	456, 733, 964, 1992
AleI	CACNNNNGTG	1 cut(s)	2032
Alw21I	GWGCWC	5 cut(s)	164, 387, 796, 1516, 2272
Alw26I	GTCTC	9 cut(s)	39, 133, 228, 744, 1008, 1439, 1475, 1501, 1952
Alw44I	GTGCAC	2 cut(s)	792, 1512
AlwI	GGATC	1 cut(s)	1624
AlwNI	CAGNNNCTG	1 cut(s)	2387
Aor51HI	AGCGCT	1 cut(s)	1422
AoxI	GGCC	7 cut(s)	139, 412, 955, 1173, 1398, 1451, 1869
ApaI	GGGCCC	2 cut(s)	143, 959
ApaLI	GTGCAC	2 cut(s)	792, 1512
ApeKI	GCWGC	9 cut(s)	425, 902, 1069, 1205, 1349, 1423, 1751, 1988, 2152
ApoI	RAATTY	7 cut(s)	538, 626, 680, 713, 1933, 2072, 2171
AseI	ATTAAT	1 cut(s)	1910
AsiGI	ACCGGT	1 cut(s)	1411
Asp700I	GAANNNNTTC	4 cut(s)	549, 564, 716, 1469
AspLEI	GCGC	5 cut(s)	1065, 1210, 1423, 1479, 2186
AspS9I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
AsuC2I	CCSGG	1 cut(s)	2193
AsuHPI	GGTGA	2 cut(s)	472, 1825
AsuNHI	GCTAGC	1 cut(s)	2342
AvaII	GGWCC	1 cut(s)	1409
BaeGI	GKGCMC	5 cut(s)	143, 355, 796, 959, 1516
BanI	GGYRCC	2 cut(s)	399, 2221
BanII	GRGCYC	2 cut(s)	143, 959
BbrPI	CACGTG	1 cut(s)	1670
BbsI	GAAGAC	2 cut(s)	567, 1695
Bbv12I	GWGCWC	5 cut(s)	164, 387, 796, 1516, 2272
BbvI	GCAGC	9 cut(s)	412, 889, 1081, 1192, 1336, 1410, 1738, 1975, 2164
BceAI	ACGGC	2 cut(s)	353, 399
BcgI	CGANNNNNNTGC	2 cut(s)	629, 663
BciT130I	CCWGG	4 cut(s)	458, 735, 966, 1994
BciVI	GTATCC	1 cut(s)	604
BclI	TGATCA	1 cut(s)	1018
BcnI	CCSGG	1 cut(s)	2193
BcoDI	GTCTC	9 cut(s)	39, 133, 228, 744, 1008, 1439, 1475, 1501, 1952
BfaI	CTAG	7 cut(s)	1509, 1521, 1568, 1875, 2156, 2343, 2397
BfoI	RGCGCY	2 cut(s)	1424, 2187
BfrI	CTTAAG	3 cut(s)	1129, 2086, 2323
BfuAI	ACCTGC	1 cut(s)	682
BfuI	GTATCC	1 cut(s)	604
BglII	AGATCT	2 cut(s)	829, 1133
Bme1390I	CCNGG	5 cut(s)	458, 735, 966, 1994, 2193
Bme18I	GGWCC	1 cut(s)	1409
BmgT120I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
BmiI	GGNNCC	6 cut(s)	141, 401, 957, 958, 1893, 2223
BmrFI	CCNGG	5 cut(s)	458, 735, 966, 1994, 2193
BmrI	ACTGGG	2 cut(s)	1291, 1689
BmsI	GCATC	7 cut(s)	196, 456, 1249, 1276, 1298, 1653, 1733
BmtI	GCTAGC	1 cut(s)	2346
BmuI	ACTGGG	2 cut(s)	1291, 1689
BpiI	GAAGAC	2 cut(s)	567, 1695
BpmI	CTGGAG	2 cut(s)	768, 1166
BpuEI	CTTGAG	1 cut(s)	724
BpuMI	CCSGG	1 cut(s)	2193
BsaAI	YACGTR	2 cut(s)	485, 1670
BsaBI	GATNNNNATC	1 cut(s)	834
BsaHI	GRCGYC	1 cut(s)	527
BsaI	GGTCTC	1 cut(s)	228
BsaJI	CCNNGG	8 cut(s)	733, 1176, 1545, 1809, 1992, 2033, 2049, 2059
BsaWI	WCCGGW	1 cut(s)	1411
Bsc4I	CCNNNNNNNGG	7 cut(s)	738, 776, 965, 971, 1639, 1810, 2318
Bse118I	RCCGGY	1 cut(s)	1411
Bse1I	ACTGG	5 cut(s)	70, 548, 1286, 1684, 2024
Bse3DI	GCAATG	3 cut(s)	726, 1332, 2185
Bse8I	GATNNNNATC	1 cut(s)	834
BseBI	CCWGG	4 cut(s)	458, 735, 966, 1994
BseDI	CCNNGG	8 cut(s)	733, 1176, 1545, 1809, 1992, 2033, 2049, 2059
BseGI	GGATG	8 cut(s)	211, 331, 1186, 1195, 1256, 1291, 1724, 1771
BseJI	GATNNNNATC	1 cut(s)	834
BseLI	CCNNNNNNNGG	7 cut(s)	738, 776, 965, 971, 1639, 1810, 2318
BseMI	GCAATG	3 cut(s)	726, 1332, 2185
BseMII	CTCAG	3 cut(s)	44, 997, 1991
BseNI	ACTGG	5 cut(s)	70, 548, 1286, 1684, 2024
BseRI	GAGGAG	2 cut(s)	333, 935
BseSI	GKGCMC	5 cut(s)	143, 355, 796, 959, 1516
BseXI	GCAGC	9 cut(s)	412, 889, 1081, 1192, 1336, 1410, 1738, 1975, 2164
BseYI	CCCAGC	1 cut(s)	1120
BsgI	GTGCAG	3 cut(s)	1275, 2171, 2289
Bsh1236I	CGCG	2 cut(s)	1479, 2165
BshFI	GGCC	7 cut(s)	141, 414, 957, 1175, 1400, 1453, 1871
BshNI	GGYRCC	2 cut(s)	399, 2221
BshTI	ACCGGT	1 cut(s)	1411
BsiHKAI	GWGCWC	5 cut(s)	164, 387, 796, 1516, 2272
BsiSI	CCGG	2 cut(s)	1412, 2192
BslFI	GGGAC	2 cut(s)	1137, 2208
BslI	CCNNNNNNNGG	7 cut(s)	738, 776, 965, 971, 1639, 1810, 2318
BsmAI	GTCTC	9 cut(s)	39, 133, 228, 744, 1008, 1439, 1475, 1501, 1952
BsmBI	CGTCTC	3 cut(s)	39, 1008, 1952
BsmFI	GGGAC	2 cut(s)	1137, 2208
BsmI	GAATGC	3 cut(s)	335, 1106, 1822
BsnI	GGCC	7 cut(s)	141, 414, 957, 1175, 1400, 1453, 1871
Bso31I	GGTCTC	1 cut(s)	228
Bsp120I	GGGCCC	2 cut(s)	139, 955
Bsp1286I	GDGCHC	8 cut(s)	143, 164, 355, 387, 796, 959, 1516, 2272
Bsp1407I	TGTACA	1 cut(s)	1921
Bsp143I	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
BspACI	CCGC	4 cut(s)	1185, 1202, 2165, 2176
BspANI	GGCC	7 cut(s)	141, 414, 957, 1175, 1400, 1453, 1871
BspCNI	CTCAG	3 cut(s)	43, 996, 1990
BspFNI	CGCG	2 cut(s)	1479, 2165
BspHI	TCATGA	2 cut(s)	2076, 2232
BspLI	GGNNCC	6 cut(s)	141, 401, 957, 958, 1893, 2223
BspMI	ACCTGC	1 cut(s)	682
BspOI	GCTAGC	1 cut(s)	2346
BspPI	GGATC	1 cut(s)	1624
BspQI	GCTCTTC	1 cut(s)	2193
BspT107I	GGYRCC	2 cut(s)	399, 2221
BspTI	CTTAAG	3 cut(s)	1129, 2086, 2323
BspTNI	GGTCTC	1 cut(s)	228
BsrDI	GCAATG	3 cut(s)	726, 1332, 2185
BsrFI	RCCGGY	1 cut(s)	1411
BsrGI	TGTACA	1 cut(s)	1921
BsrI	ACTGG	5 cut(s)	70, 548, 1286, 1684, 2024
BssAI	RCCGGY	1 cut(s)	1411
BssECI	CCNNGG	8 cut(s)	733, 1176, 1545, 1809, 1992, 2033, 2049, 2059
BssMI	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
BssNI	GRCGYC	1 cut(s)	527
BssT1I	CCWWGG	4 cut(s)	1176, 1545, 1809, 2049
Bst2UI	CCWGG	4 cut(s)	458, 735, 966, 1994
Bst4CI	ACNGT	6 cut(s)	78, 451, 868, 1858, 1926, 1970
Bst6I	CTCTTC	5 cut(s)	669, 873, 1605, 2193, 2240
BstACI	GRCGYC	1 cut(s)	527
BstAFI	CTTAAG	3 cut(s)	1129, 2086, 2323
BstAPI	GCANNNNNTGC	2 cut(s)	257, 2276
BstAUI	TGTACA	1 cut(s)	1921
BstBAI	YACGTR	2 cut(s)	485, 1670
BstC8I	GCNNGC	7 cut(s)	423, 700, 744, 900, 1067, 1455, 2344
BstDEI	CTNAG	5 cut(s)	30, 554, 620, 983, 1977
BstDSI	CCRYGG	1 cut(s)	2033
BstENI	CCTNNNNNAGG	1 cut(s)	774
BstF5I	GGATG	8 cut(s)	211, 331, 1186, 1195, 1256, 1291, 1724, 1771
BstFNI	CGCG	2 cut(s)	1479, 2165
BstH2I	RGCGCY	2 cut(s)	1424, 2187
BstHHI	GCGC	5 cut(s)	1065, 1210, 1423, 1479, 2186
BstKTI	GATC	5 cut(s)	832, 838, 1021, 1136, 1632
BstMAI	GTCTC	9 cut(s)	39, 133, 228, 744, 1008, 1439, 1475, 1501, 1952
BstMBI	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
BstNI	CCWGG	4 cut(s)	458, 735, 966, 1994
BstNSI	RCATGY	2 cut(s)	397, 902
BstSCI	CCNGG	5 cut(s)	456, 733, 964, 1992, 2191
BstSLI	GKGCMC	5 cut(s)	143, 355, 796, 959, 1516
BstUI	CGCG	2 cut(s)	1479, 2165
BstV1I	GCAGC	9 cut(s)	412, 889, 1081, 1192, 1336, 1410, 1738, 1975, 2164
BstV2I	GAAGAC	2 cut(s)	567, 1695
BstX2I	RGATCY	2 cut(s)	829, 1133
BstXI	CCANNNNNNTGG	4 cut(s)	410, 1674, 2034, 2218
BstYI	RGATCY	2 cut(s)	829, 1133
BsuI	GTATCC	1 cut(s)	604
BsuRI	GGCC	7 cut(s)	141, 414, 957, 1175, 1400, 1453, 1871
BtgI	CCRYGG	1 cut(s)	2033
BtgZI	GCGATG	1 cut(s)	2146
BtsCI	GGATG	8 cut(s)	211, 331, 1186, 1195, 1256, 1291, 1724, 1771
BtsI	GCAGTG	2 cut(s)	23, 2335
BtsIMutI	CAGTG	6 cut(s)	23, 63, 796, 1931, 1975, 2335
BveI	ACCTGC	1 cut(s)	682
Cac8I	GCNNGC	7 cut(s)	423, 700, 744, 900, 1067, 1455, 2344
CaiI	CAGNNNCTG	1 cut(s)	2387
CciI	TCATGA	2 cut(s)	2076, 2232
CfoI	GCGC	5 cut(s)	1065, 1210, 1423, 1479, 2186
Cfr10I	RCCGGY	1 cut(s)	1411
Cfr13I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
CseI	GACGC	3 cut(s)	535, 2268, 2355
Csp6I	GTAC	5 cut(s)	276, 300, 869, 1922, 1966
CspAI	ACCGGT	1 cut(s)	1411
CviQI	GTAC	5 cut(s)	276, 300, 869, 1922, 1966
DdeI	CTNAG	5 cut(s)	30, 554, 620, 983, 1977
DpnI	GATC	5 cut(s)	831, 837, 1020, 1135, 1631
DpnII	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
DraI	TTTAAA	1 cut(s)	685
EaeI	YGGCCR	1 cut(s)	412
Eam1104I	CTCTTC	5 cut(s)	669, 873, 1605, 2193, 2240
EarI	CTCTTC	5 cut(s)	669, 873, 1605, 2193, 2240
Eco130I	CCWWGG	4 cut(s)	1176, 1545, 1809, 2049
Eco147I	AGGCCT	2 cut(s)	1175, 1400
Eco24I	GRGCYC	2 cut(s)	143, 959
Eco31I	GGTCTC	1 cut(s)	228
Eco47I	GGWCC	1 cut(s)	1409
Eco47III	AGCGCT	1 cut(s)	1422
Eco57I	CTGAAG	2 cut(s)	618, 759
Eco72I	CACGTG	1 cut(s)	1670
EcoNI	CCTNNNNNAGG	1 cut(s)	774
EcoO109I	RGGNCCY	1 cut(s)	956
EcoRI	GAATTC	2 cut(s)	2072, 2171
EcoRII	CCWGG	4 cut(s)	456, 733, 964, 1992
EcoT14I	CCWWGG	4 cut(s)	1176, 1545, 1809, 2049
EcoT22I	ATGCAT	1 cut(s)	1291
EcoT38I	GRGCYC	2 cut(s)	143, 959
ErhI	CCWWGG	4 cut(s)	1176, 1545, 1809, 2049
Esp3I	CGTCTC	3 cut(s)	39, 1008, 1952
FalI	AAGNNNNNCTT	6 cut(s)	514, 546, 1385, 1417, 1410, 1442
FaqI	GGGAC	2 cut(s)	1137, 2208
FauI	CCCGC	1 cut(s)	1178
FauNDI	CATATG	1 cut(s)	1576
FbaI	TGATCA	1 cut(s)	1018
FokI	GGATG	8 cut(s)	218, 338, 1193, 1202, 1263, 1298, 1711, 1778
FriOI	GRGCYC	2 cut(s)	143, 959
FspBI	CTAG	7 cut(s)	1509, 1521, 1568, 1875, 2156, 2343, 2397
FspI	TGCGCA	1 cut(s)	1209
GlaI	GCGC	5 cut(s)	1064, 1209, 1422, 1478, 2185
GsaI	CCCAGC	1 cut(s)	1124
GsuI	CTGGAG	2 cut(s)	768, 1166
HaeII	RGCGCY	2 cut(s)	1424, 2187
HaeIII	GGCC	7 cut(s)	141, 414, 957, 1175, 1400, 1453, 1871
HapII	CCGG	2 cut(s)	1412, 2192
HgaI	GACGC	3 cut(s)	535, 2268, 2355
HhaI	GCGC	5 cut(s)	1065, 1210, 1423, 1479, 2186
Hin1I	GRCGYC	1 cut(s)	527
Hin6I	GCGC	5 cut(s)	1063, 1208, 1421, 1477, 2184
HinP1I	GCGC	5 cut(s)	1063, 1208, 1421, 1477, 2184
HincII	GTYRAC	1 cut(s)	1861
HindII	GTYRAC	1 cut(s)	1861
HindIII	AAGCTT	1 cut(s)	1113
HpaII	CCGG	2 cut(s)	1412, 2192
HphI	GGTGA	2 cut(s)	472, 1825
Hpy166II	GTNNAC	6 cut(s)	110, 482, 794, 1514, 1861, 2211
Hpy188III	TCNNGA	9 cut(s)	340, 512, 833, 1022, 1145, 1407, 1568, 2077, 2233
Hpy8I	GTNNAC	6 cut(s)	110, 482, 794, 1514, 1861, 2211
HpyAV	CCTTC	8 cut(s)	213, 1037, 1387, 1614, 1633, 1678, 2255, 2283
HpyCH4III	ACNGT	6 cut(s)	78, 451, 868, 1858, 1926, 1970
HpyCH4IV	ACGT	6 cut(s)	484, 871, 945, 1001, 1315, 1669
HpyF3I	CTNAG	5 cut(s)	30, 554, 620, 983, 1977
HpySE526I	ACGT	6 cut(s)	484, 871, 945, 1001, 1315, 1669
Hsp92I	GRCGYC	1 cut(s)	527
HspAI	GCGC	5 cut(s)	1063, 1208, 1421, 1477, 2184
Ksp22I	TGATCA	1 cut(s)	1018
Kzo9I	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
LguI	GCTCTTC	1 cut(s)	2193
LmnI	GCTCC	4 cut(s)	937, 1433, 2267, 2333
Lsp1109I	GCAGC	9 cut(s)	412, 889, 1081, 1192, 1336, 1410, 1738, 1975, 2164
LweI	GCATC	7 cut(s)	196, 456, 1249, 1276, 1298, 1653, 1733
MaeI	CTAG	7 cut(s)	1509, 1521, 1568, 1875, 2156, 2343, 2397
MaeII	ACGT	6 cut(s)	484, 871, 945, 1001, 1315, 1669
MaeIII	GTNAC	1 cut(s)	2377
MalI	GATC	5 cut(s)	831, 837, 1020, 1135, 1631
MboI	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
MfeI	CAATTG	1 cut(s)	950
MflI	RGATCY	2 cut(s)	829, 1133
MhlI	GDGCHC	8 cut(s)	143, 164, 355, 387, 796, 959, 1516, 2272
MlyI	GAGTC	3 cut(s)	105, 937, 1549
MmeI	TCCRAC	2 cut(s)	915, 1743
Mph1103I	ATGCAT	1 cut(s)	1291
MroXI	GAANNNNTTC	4 cut(s)	549, 564, 716, 1469
MseI	TTAA	9 cut(s)	633, 684, 1130, 1311, 1910, 2067, 2087, 2136, 2324
MslI	CAYNNNNRTG	3 cut(s)	392, 2032, 2237
MspA1I	CMGCKG	1 cut(s)	1493
MspCI	CTTAAG	3 cut(s)	1129, 2086, 2323
MspI	CCGG	2 cut(s)	1412, 2192
MspR9I	CCNGG	5 cut(s)	458, 735, 966, 1994, 2193
MunI	CAATTG	1 cut(s)	950
Mva1269I	GAATGC	3 cut(s)	335, 1106, 1822
MvaI	CCWGG	4 cut(s)	458, 735, 966, 1994
MvnI	CGCG	2 cut(s)	1479, 2165
NciI	CCSGG	1 cut(s)	2193
NdeI	CATATG	1 cut(s)	1576
NdeII	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
NheI	GCTAGC	1 cut(s)	2342
NlaIV	GGNNCC	6 cut(s)	141, 401, 957, 958, 1893, 2223
NmuCI	GTSAC	1 cut(s)	2377
NsbI	TGCGCA	1 cut(s)	1209
NsiI	ATGCAT	1 cut(s)	1291
NspI	RCATGY	2 cut(s)	397, 902
OliI	CACNNNNGTG	1 cut(s)	2032
PaeI	GCATGC	1 cut(s)	902
PagI	TCATGA	2 cut(s)	2076, 2232
PceI	AGGCCT	2 cut(s)	1175, 1400
PciI	ACATGT	1 cut(s)	393
PciSI	GCTCTTC	1 cut(s)	2193
PctI	GAATGC	3 cut(s)	335, 1106, 1822
PdmI	GAANNNNTTC	4 cut(s)	549, 564, 716, 1469
PfeI	GAWTC	7 cut(s)	69, 581, 1223, 1487, 1652, 1658, 2384
PfoI	TCCNGGA	1 cut(s)	2191
PinAI	ACCGGT	1 cut(s)	1411
PleI	GAGTC	3 cut(s)	105, 936, 1549
PmaCI	CACGTG	1 cut(s)	1670
PmlI	CACGTG	1 cut(s)	1670
PpsI	GAGTC	3 cut(s)	105, 936, 1549
Ppu21I	YACGTR	2 cut(s)	485, 1670
PscI	ACATGT	1 cut(s)	393
PshBI	ATTAAT	1 cut(s)	1910
PsiI	TTATAA	1 cut(s)	83
Psp6I	CCWGG	4 cut(s)	456, 733, 964, 1992
PspCI	CACGTG	1 cut(s)	1670
PspFI	CCCAGC	1 cut(s)	1120
PspGI	CCWGG	4 cut(s)	456, 733, 964, 1992
PspN4I	GGNNCC	6 cut(s)	141, 401, 957, 958, 1893, 2223
PspOMI	GGGCCC	2 cut(s)	139, 955
PspPI	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
PstNI	CAGNNNCTG	1 cut(s)	2387
PsuI	RGATCY	2 cut(s)	829, 1133
PvuII	CAGCTG	1 cut(s)	1493
RsaI	GTAC	5 cut(s)	277, 301, 870, 1923, 1967
RsaNI	GTAC	5 cut(s)	276, 300, 869, 1922, 1966
RseI	CAYNNNNRTG	3 cut(s)	392, 2032, 2237
SapI	GCTCTTC	1 cut(s)	2193
SaqAI	TTAA	9 cut(s)	633, 684, 1130, 1311, 1910, 2067, 2087, 2136, 2324
Sau3AI	GATC	5 cut(s)	829, 835, 1018, 1133, 1629
Sau96I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
SchI	GAGTC	3 cut(s)	105, 937, 1549
ScrFI	CCNGG	5 cut(s)	458, 735, 966, 1994, 2193
SduI	GDGCHC	8 cut(s)	143, 164, 355, 387, 796, 959, 1516, 2272
SfaNI	GCATC	7 cut(s)	196, 456, 1249, 1276, 1298, 1653, 1733
SinI	GGWCC	1 cut(s)	1409
SmiMI	CAYNNNNRTG	3 cut(s)	392, 2032, 2237
SmlI	CTYRAG	4 cut(s)	703, 1129, 2086, 2323
SmoI	CTYRAG	4 cut(s)	703, 1129, 2086, 2323
SphI	GCATGC	1 cut(s)	902
SseBI	AGGCCT	2 cut(s)	1175, 1400
SsiI	CCGC	4 cut(s)	1185, 1202, 2165, 2176
SspI	AATATT	1 cut(s)	1786
SspMI	CTAG	7 cut(s)	1509, 1521, 1568, 1875, 2156, 2343, 2397
StuI	AGGCCT	2 cut(s)	1175, 1400
StyD4I	CCNGG	5 cut(s)	456, 733, 964, 1992, 2191
StyI	CCWWGG	4 cut(s)	1176, 1545, 1809, 2049
TaaI	ACNGT	6 cut(s)	78, 451, 868, 1858, 1926, 1970
TaiI	ACGT	6 cut(s)	487, 874, 948, 1004, 1318, 1672
TaqI	TCGA	5 cut(s)	72, 339, 639, 1661, 2351
TatI	WGTACW	3 cut(s)	275, 1921, 1965
TauI	GCSGC	2 cut(s)	1205, 2168
TfiI	GAWTC	7 cut(s)	69, 581, 1223, 1487, 1652, 1658, 2384
Tru1I	TTAA	9 cut(s)	633, 684, 1130, 1311, 1910, 2067, 2087, 2136, 2324
Tru9I	TTAA	9 cut(s)	633, 684, 1130, 1311, 1910, 2067, 2087, 2136, 2324
TscAI	CASTG	6 cut(s)	23, 70, 796, 1931, 1975, 2335
TseFI	GTSAC	1 cut(s)	2377
TseI	GCWGC	9 cut(s)	425, 902, 1069, 1205, 1349, 1423, 1751, 1988, 2152
Tsp45I	GTSAC	1 cut(s)	2377
TspDTI	ATGAA	8 cut(s)	678, 1056, 1652, 2065, 2085, 2249, 2258, 2274
TspGWI	ACGGA	4 cut(s)	1330, 1622, 1976, 2022
TspRI	CASTG	6 cut(s)	23, 70, 796, 1931, 1975, 2335
Vha464I	CTTAAG	3 cut(s)	1129, 2086, 2323
VneI	GTGCAC	2 cut(s)	792, 1512
VpaK11BI	GGWCC	1 cut(s)	1409
VspI	ATTAAT	1 cut(s)	1910
XagI	CCTNNNNNAGG	1 cut(s)	774
XapI	RAATTY	7 cut(s)	538, 626, 680, 713, 1933, 2072, 2171
XbaI	TCTAGA	1 cut(s)	1567
XceI	RCATGY	2 cut(s)	397, 902
XmnI	GAANNNNTTC	4 cut(s)	549, 564, 716, 1469
XspI	CTAG	7 cut(s)	1509, 1521, 1568, 1875, 2156, 2343, 2397
Zsp2I	ATGCAT	1 cut(s)	1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.