Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0004898

Physical Location & Seq

Genomic Coordinates Reverse (-)

24036313 .. 24051061

Gene Structure 14749 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00102050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGATCGATCGGCGAGCTTGTTCGAACTCAACCAAGGAAGCCCGAAGCTTTGCGACGTCGGAGCTGCATTTTCCGGCCGGGTCGTGCTACTTCAAGCGTGCGGACTTGCGGCGCCGCCGTGGGTTCGAATCGTCGACGGAGACCACCACGGAGACGACCGGAGCAAGGAGGCGACCCATTCGGAAAGTTTCGTCGCCGGAGGTGGCCGGAGGCTGCAAGTTCCGGCCGGGTCGGATCACCGAGTCCGGGTCGGGTCGGGCGGAAAACTTCGATTCGAAGCAAAGAGTGAGCAGTGCTGCTCTACTCAGCTTATTGATGGAGACGGATCATTCAATGACACTGGAATCGAACAGTTTATAAAGGAGGTGAAATTGGGAGAATGTGGACTCTCCTATGCAGTAGTCTCCATTATGGGCCCTCAAAGTAGTGGAAAGAGCACACTACTAAATAATTTGTTTGCTACCAACTTCAAGGAGATGGATGCTTTTAGAGGAAGGCAAGTTCGCATAATGCTCAATACAACAAAAGGTATTTGGTTGGCAAAATGTGCTGGTATTGAGCCTTGTACTCTTGTAATGGATTTGGAGGGTACAGATGGGAGAGAACGAGGAGAGGATGATACGGCATTCGAGAAGCAGAGTGCCCTCTTTGCCCTTGCTGTTTCAGATATAGTTCTCATTAACATGTGGTGCCATGATATTGGCCGTGAGCAAGCTGCAAATAAGCCTCTCCTAAAGACTGTGTTCCAGGTGATGATGCGATTGTTTAGTCCACGTAAAACAACTCTGATGTTCGTCATCCGTGATAAGACAAGGACACCTTTAGAGAATTTAGAACCGGTTCTTAGAGAAGACATTCAGAAGATATGGGATTCTGTTCCGAAGCCTGAAGCCCACAAGGATACTCCACTAAGTGAATTTTTTAATGTCGAGGTTGTTGCTCTTTCAAGTTATGAAGAAAAAGAAGAGCAATTTAAAGAGCAGACGGGGGTAGTTCCTGCTTCAGGGTTTTCTTTTAGTGCACAGCAGATATGGAGAGTCATCAAAGAAAACAGAGATCTTGATCTTCCTGCCCACAAGGTCATGGTGGCAACTGTACGTTGTGAAGAGATTGCCAACGAGAAGCATGCTGCTTTTGTAGGAAATGAGGAGTGGAGTCAGTTTGAGCAGGATGTTCAATTGGGCCCTATTCCTGGCTTTGGGAAGAAGCTCAGTTCAATCATTGATACGTGTCTATCAGGGTATGATGAAGAAGCTACATACTTTGATGAAGGTGTCAGATCTGGGAAGCGCAAGCAGCTTGAAGAAAAATTGCTTCAACATGTCCAACCTGCATTCCAAGCTTTGCTGGGACACTTAAGATCTGGTACTCTGGATAAGTTCAAGGAAGCATTTGATAAGGCCTTGGATGGCGGGCAGGGTTTTTCTGTGGCTGCGCATAACTGCAGTGAATCTTTCATGGCTCAATTTGATGAAGGATGTGCAGATGCTGTTATCCAACAAGCAGATTGGGACACATCTAAAGTAAGAGATAAACTTAAACGTGATATAGATGCTCACATTGCTTCAGTTCGTGATGCTAAGCTATCCGAAATTACGACCCTTTATGAGGCAAAATTGAAGGAGGCCCTATCTGGACCCGTGGAAGCGCTACTTGATGGAGCTAATAGTGAGACTTGGCCTGCAATAAGGAAACTTTTCAAGCGCGAGACTGAATCAGCTGTCTCTGGGTTCTCTAGTGCACTTTCTGGTTTTGATATGGATAGACAAACCAAGGATAAAATTCTTGCAAGTCTAGAGGCATATTCAAGAGGTGTAGTGGAAGCAAAAACAAAGGAAGAGGCTGGAAGGGTTTTGATCCGTATGAAGGACAGGTTTGCAACACTATTTAGCCACGACTCTGATTCGATGCCACGTGTATGGACTGGGAAGGAAGACATCAGAGCAATTACCAAGACTGCTCGTTCTGCATCCCTGAAATTGCTTTCTGTTATGGCTGCAATTCGGTTGGATGATGGTGATGCTGATAATATTGAGAAAACTTTATCTCTTGCCTTGGTGGATGGCAGAAATGCTGCTCCCAAAGATAAGAGTATCACAACGGTTGACCCATTGGCCTCTAGCACTTGGCAAGAGGAAGCCAACAAGCGTAATAACAATTGGCTACCACCTCCTTGGGCTATTCTTGCCTTGGTTGTCCTTGGATTTAATGAATTCATGACACTCTTAAGAAATCCTTTGTATCTGCTGGTCATCTTTGTTGGCTTTTTACTCCTTAAAGCACTGTGGGTGCAGCTAGACATCTCGGCTGAATTCCGCAATGGCGCTCTTCCGGGACTTCTCTCTTTGTCCACCAAGTTGGTGCCAACTATCATGAACATGATGAAGAGATTAGCAGATGAAGGGGCAGCAGCTGCTGCAGATAACAATCCTCAAAGAAACCCTGCAGTAGCATCAAAAGGCATAAGCAATGGAGCAAATGCTAGCAGTTCCATGTCAACTTCTGCCTCGTCGGGAGTGACAGAATCTGAATACTCTAGTCCTTCAAAACAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000902 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0006810 GO:0006888 GO:0006996 GO:0007010 GO:0007275 GO:0008150 GO:0009653 GO:0009826 GO:0009832 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0017076 GO:0019001 GO:0022622 GO:0030029 GO:0030036 GO:0030154 GO:0031090 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032989 GO:0035639 GO:0036094 GO:0040007 GO:0042546 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048364 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060560 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:0099402 GO:1901265 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RHD3

Protein Analysis

852

Amino Acids

93.57

Weight (kDa)

5.54

Isoelectric Point (pI)

37.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
URGCP_GTPase	PF25683	103 - 266	5.2e-09	URGCP-like GTPase domain
RHD3_GTPase	PF05879	138 - 328	3.1e-91	Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
RHD3_GTPase	PF05879	329 - 357	4.1e-07	Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB	PF20428	399 - 715	1.3e-110	Sey1 three-helix bundle domain
Sey1_3HB	PF20428	716 - 801	3.8e-21	Sey1 three-helix bundle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13870 AT3G13870
fragaria_vesca	FvH4_6g33350 FvH4_6g33350 FvH4_6g33350 FvH4_6g33350
malus_domestica	MD09G1193400.v1.1 MD17G1174200.v1.1
prunus_persica	Prupe.3G049800_v2.0.a1
pyrus_communis	pycom09g10990 pycom17g17830
rosa_chinensis	RchiOBHm_Chr2g0143201 RchiOBHm_Chr2g0169871
rosa_laevigata	RLG00000020013
rosa_multiflora	Rmu_co8092052.1_g000001 Rmu_sc0000181.1_g000004 Rmu_sc0003950.1_g000012
rosa_roxburghii	Rroxscaffold_2G00102050 Rroxscaffold_5G00359600 Rroxscaffold_7G00190950 Rroxscaffold_7G00191100
rosa_rugosa	Rorug02G0379200
rosa_samantha	Rh1BG269400 Rh2AG432000 Rh2BG440600 Rh2CG385100 Rh2CG417900 Rh2DG450800 Rh7AG446900
rosa_wichuraiana	Rw2G035290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	359
AatII	GACGTC	1 cut(s)	60
Acc16I	TGCGCA	1 cut(s)	1437
Acc36I	ACCTGC	1 cut(s)	1339
AccB1I	GGYRCC	3 cut(s)	112, 690, 2362
AccI	GTMKAC	1 cut(s)	135
AccII	CGCG	1 cut(s)	1707
AciI	CCGC	6 cut(s)	102, 110, 116, 261, 1413, 2317
AclWI	GGATC	4 cut(s)	12, 243, 334, 1852
AcoI	YGGCCR	4 cut(s)	76, 205, 225, 703
AcsI	RAATTY	5 cut(s)	829, 917, 1782, 2213, 2312
AcuI	CTGAAG	3 cut(s)	909, 987, 1551
AcvI	CACGTG	1 cut(s)	1916
AcyI	GRCGYC	2 cut(s)	57, 113
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	4 cut(s)	568, 592, 1098, 1369
AfeI	AGCGCT	1 cut(s)	1650
AfiI	CCNNNNNNNGG	7 cut(s)	166, 247, 1004, 1193, 1199, 1609, 1867
AflII	CTTAAG	2 cut(s)	1357, 2227
AflIII	ACRYGT	4 cut(s)	684, 1229, 1321, 1915
AgeI	ACCGGT	1 cut(s)	838
AjnI	CCWGG	2 cut(s)	747, 1192
AloI	GAACNNNNNNTCC	2 cut(s)	486, 518
Alw21I	GWGCWC	3 cut(s)	440, 1024, 1744
Alw26I	GTCTC	7 cut(s)	135, 147, 315, 409, 1667, 1703, 1729
Alw44I	GTGCAC	2 cut(s)	1020, 1740
AlwI	GGATC	4 cut(s)	12, 243, 334, 1852
AlwNI	CAGNNNCTG	4 cut(s)	1490, 2414, 2417, 2528
Aor51HI	AGCGCT	1 cut(s)	1650
ApaI	GGGCCC	2 cut(s)	419, 1187
ApaLI	GTGCAC	2 cut(s)	1020, 1740
ApoI	RAATTY	5 cut(s)	829, 917, 1782, 2213, 2312
AsiGI	ACCGGT	1 cut(s)	838
Asp700I	GAANNNNTTC	3 cut(s)	840, 855, 1697
AspLEI	GCGC	6 cut(s)	115, 1293, 1438, 1651, 1707, 2327
AspS9I	GGNCC	6 cut(s)	415, 416, 1183, 1184, 1627, 1637
AsuC2I	CCSGG	4 cut(s)	80, 229, 248, 2334
AsuHPI	GGTGA	4 cut(s)	230, 379, 763, 2030
AsuII	TTCGAA	3 cut(s)	24, 127, 276
AsuNHI	GCTAGC	1 cut(s)	2483
AvaII	GGWCC	1 cut(s)	1637
BaeGI	GKGCMC	5 cut(s)	419, 646, 1024, 1187, 1744
BanI	GGYRCC	3 cut(s)	112, 690, 2362
BanII	GRGCYC	2 cut(s)	419, 1187
BbrPI	CACGTG	1 cut(s)	1916
BbsI	GAAGAC	2 cut(s)	858, 1941
Bbv12I	GWGCWC	3 cut(s)	440, 1024, 1744
BccI	CCATC	7 cut(s)	311, 472, 590, 1403, 1652, 2009, 2057
BceAI	ACGGC	3 cut(s)	103, 639, 690
BcgI	CGANNNNNNTGC	4 cut(s)	920, 954, 1109, 1143
BciT130I	CCWGG	2 cut(s)	749, 1194
BciVI	GTATCC	1 cut(s)	895
BcnI	CCSGG	4 cut(s)	80, 229, 248, 2334
BcoDI	GTCTC	7 cut(s)	135, 147, 315, 409, 1667, 1703, 1729
BfaI	CTAG	6 cut(s)	1737, 1796, 2121, 2297, 2484, 2538
BfmI	CTRYAG	3 cut(s)	1444, 2418, 2445
BfoI	RGCGCY	3 cut(s)	116, 1652, 2328
BfrI	CTTAAG	2 cut(s)	1357, 2227
BfuAI	ACCTGC	1 cut(s)	1339
BfuI	GTATCC	1 cut(s)	895
BglII	AGATCT	3 cut(s)	1057, 1280, 1361
BlpI	GCTNAGC	1 cut(s)	1581
Bme1390I	CCNGG	6 cut(s)	80, 229, 248, 749, 1194, 2334
Bme18I	GGWCC	1 cut(s)	1637
BmgT120I	GGNCC	6 cut(s)	415, 416, 1183, 1184, 1627, 1637
BmiI	GGNNCC	6 cut(s)	114, 417, 692, 1185, 1639, 2364
BmrFI	CCNGG	6 cut(s)	80, 229, 248, 749, 1194, 2334
BmrI	ACTGGG	1 cut(s)	1935
BmsI	GCATC	9 cut(s)	472, 747, 1477, 1543, 1567, 1899, 1979, 2011, 2462
BmtI	GCTAGC	1 cut(s)	2487
BmuI	ACTGGG	1 cut(s)	1935
BpiI	GAAGAC	2 cut(s)	858, 1941
Bpu1102I	GCTNAGC	1 cut(s)	1581
Bpu14I	TTCGAA	3 cut(s)	24, 127, 276
BpuMI	CCSGG	4 cut(s)	80, 229, 248, 2334
Bsa29I	ATCGAT	1 cut(s)	7
BsaAI	YACGTR	3 cut(s)	776, 1230, 1916
BsaBI	GATNNNNATC	2 cut(s)	1062, 2427
BsaHI	GRCGYC	2 cut(s)	57, 113
BsaI	GGTCTC	1 cut(s)	135
BsaWI	WCCGGW	2 cut(s)	159, 838
Bsc4I	CCNNNNNNNGG	7 cut(s)	166, 247, 1004, 1193, 1199, 1609, 1867
Bse118I	RCCGGY	1 cut(s)	838
Bse1I	ACTGG	2 cut(s)	346, 1930
Bse3DI	GCAATG	3 cut(s)	1560, 2326, 2476
Bse8I	GATNNNNATC	2 cut(s)	1062, 2427
BseBI	CCWGG	2 cut(s)	749, 1194
BseCI	ATCGAT	1 cut(s)	7
BseGI	GGATG	9 cut(s)	487, 622, 798, 1177, 1414, 1484, 1970, 2017, 2068
BseJI	GATNNNNATC	2 cut(s)	1062, 2427
BseLI	CCNNNNNNNGG	7 cut(s)	166, 247, 1004, 1193, 1199, 1609, 1867
BseMI	GCAATG	3 cut(s)	1560, 2326, 2476
BseMII	CTCAG	2 cut(s)	320, 1225
BseNI	ACTGG	2 cut(s)	346, 1930
BseRI	GAGGAG	2 cut(s)	624, 1163
BseSI	GKGCMC	5 cut(s)	419, 646, 1024, 1187, 1744
BseX3I	CGGCCG	2 cut(s)	76, 225
BseYI	CCCAGC	1 cut(s)	1348
BsgI	GTGCAG	2 cut(s)	1503, 2312
Bsh1236I	CGCG	1 cut(s)	1707
Bsh1285I	CGRYCG	4 cut(s)	11, 79, 160, 228
BshNI	GGYRCC	3 cut(s)	112, 690, 2362
BshTI	ACCGGT	1 cut(s)	838
BshVI	ATCGAT	1 cut(s)	7
BsiEI	CGRYCG	4 cut(s)	11, 79, 160, 228
BsiHKAI	GWGCWC	3 cut(s)	440, 1024, 1744
BslFI	GGGAC	3 cut(s)	1365, 1526, 2349
BslI	CCNNNNNNNGG	7 cut(s)	166, 247, 1004, 1193, 1199, 1609, 1867
BsmAI	GTCTC	7 cut(s)	135, 147, 315, 409, 1667, 1703, 1729
BsmBI	CGTCTC	2 cut(s)	147, 315
BsmFI	GGGAC	3 cut(s)	1365, 1526, 2349
BsmI	GAATGC	2 cut(s)	626, 1334
Bso31I	GGTCTC	1 cut(s)	135
Bsp119I	TTCGAA	3 cut(s)	24, 127, 276
Bsp120I	GGGCCC	2 cut(s)	415, 1183
Bsp1286I	GDGCHC	6 cut(s)	419, 440, 646, 1024, 1187, 1744
Bsp143I	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
Bsp1720I	GCTNAGC	1 cut(s)	1581
BspACI	CCGC	6 cut(s)	102, 110, 116, 261, 1413, 2317
BspCNI	CTCAG	2 cut(s)	319, 1224
BspDI	ATCGAT	1 cut(s)	7
BspFNI	CGCG	1 cut(s)	1707
BspHI	TCATGA	2 cut(s)	2217, 2373
BspLI	GGNNCC	6 cut(s)	114, 417, 692, 1185, 1639, 2364
BspMAI	CTGCAG	3 cut(s)	1448, 2422, 2449
BspMI	ACCTGC	1 cut(s)	1339
BspOI	GCTAGC	1 cut(s)	2487
BspPI	GGATC	4 cut(s)	12, 243, 334, 1852
BspQI	GCTCTTC	2 cut(s)	960, 2334
BspT104I	TTCGAA	3 cut(s)	24, 127, 276
BspT107I	GGYRCC	3 cut(s)	112, 690, 2362
BspTI	CTTAAG	2 cut(s)	1357, 2227
BspTNI	GGTCTC	1 cut(s)	135
BsrDI	GCAATG	3 cut(s)	1560, 2326, 2476
BsrFI	RCCGGY	1 cut(s)	838
BsrI	ACTGG	2 cut(s)	346, 1930
BssAI	RCCGGY	1 cut(s)	838
BssMI	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
BssNI	GRCGYC	2 cut(s)	57, 113
BssT1I	CCWWGG	7 cut(s)	34, 1404, 1773, 2055, 2174, 2190, 2200
Bst2UI	CCWGG	2 cut(s)	749, 1194
Bst4CI	ACNGT	5 cut(s)	354, 742, 1096, 2104, 2286
Bst6I	CTCTTC	5 cut(s)	960, 1101, 1833, 2334, 2381
BstACI	GRCGYC	2 cut(s)	57, 113
BstAFI	CTTAAG	2 cut(s)	1357, 2227
BstAPI	GCANNNNNTGC	3 cut(s)	548, 2414, 2417
BstBAI	YACGTR	3 cut(s)	776, 1230, 1916
BstBI	TTCGAA	3 cut(s)	24, 127, 276
BstC8I	GCNNGC	8 cut(s)	16, 100, 714, 1128, 1295, 1415, 1683, 2485
BstDEI	CTNAG	5 cut(s)	306, 845, 911, 1211, 1581
BstDSI	CCRYGG	3 cut(s)	119, 148, 1641
BstENI	CCTNNNNNAGG	2 cut(s)	1002, 1607
BstF5I	GGATG	9 cut(s)	487, 622, 798, 1177, 1414, 1484, 1970, 2017, 2068
BstFNI	CGCG	1 cut(s)	1707
BstH2I	RGCGCY	3 cut(s)	116, 1652, 2328
BstHHI	GCGC	6 cut(s)	115, 1293, 1438, 1651, 1707, 2327
BstKTI	GATC	9 cut(s)	7, 11, 238, 329, 1060, 1066, 1283, 1364, 1860
BstMAI	GTCTC	7 cut(s)	135, 147, 315, 409, 1667, 1703, 1729
BstMBI	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
BstMCI	CGRYCG	4 cut(s)	11, 79, 160, 228
BstMWI	GCNNNNNNNGC	9 cut(s)	548, 650, 1297, 1340, 1562, 1967, 2186, 2414, 2417
BstNI	CCWGG	2 cut(s)	749, 1194
BstNSI	RCATGY	3 cut(s)	688, 1130, 1325
BstSCI	CCNGG	6 cut(s)	78, 227, 246, 747, 1192, 2332
BstSFI	CTRYAG	3 cut(s)	1444, 2418, 2445
BstSLI	GKGCMC	5 cut(s)	419, 646, 1024, 1187, 1744
BstUI	CGCG	1 cut(s)	1707
BstV2I	GAAGAC	2 cut(s)	858, 1941
BstX2I	RGATCY	3 cut(s)	1057, 1280, 1361
BstXI	CCANNNNNNTGG	4 cut(s)	701, 1920, 2175, 2359
BstYI	RGATCY	3 cut(s)	1057, 1280, 1361
BstZI	CGGCCG	2 cut(s)	76, 225
Bsu15I	ATCGAT	1 cut(s)	7
BsuI	GTATCC	1 cut(s)	895
BsuTUI	ATCGAT	1 cut(s)	7
BtgI	CCRYGG	3 cut(s)	119, 148, 1641
BtsCI	GGATG	9 cut(s)	487, 622, 798, 1177, 1414, 1484, 1970, 2017, 2068
BtsI	GCAGTG	2 cut(s)	299, 1453
BtsIMutI	CAGTG	4 cut(s)	299, 339, 1453, 2282
BveI	ACCTGC	1 cut(s)	1339
Cac8I	GCNNGC	8 cut(s)	16, 100, 714, 1128, 1295, 1415, 1683, 2485
CaiI	CAGNNNCTG	4 cut(s)	1490, 2414, 2417, 2528
CciI	TCATGA	2 cut(s)	2217, 2373
CfoI	GCGC	6 cut(s)	115, 1293, 1438, 1651, 1707, 2327
Cfr10I	RCCGGY	1 cut(s)	838
Cfr13I	GGNCC	6 cut(s)	415, 416, 1183, 1184, 1627, 1637
ClaI	ATCGAT	1 cut(s)	7
Csp6I	GTAC	4 cut(s)	567, 591, 1097, 1368
CspAI	ACCGGT	1 cut(s)	838
CviQI	GTAC	4 cut(s)	567, 591, 1097, 1368
DdeI	CTNAG	5 cut(s)	306, 845, 911, 1211, 1581
DinI	GGCGCC	1 cut(s)	114
DpnI	GATC	9 cut(s)	6, 10, 237, 328, 1059, 1065, 1282, 1363, 1859
DpnII	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
DraI	TTTAAA	1 cut(s)	976
DraIII	CACNNNGTG	1 cut(s)	914
EaeI	YGGCCR	4 cut(s)	76, 205, 225, 703
EagI	CGGCCG	2 cut(s)	76, 225
Eam1104I	CTCTTC	5 cut(s)	960, 1101, 1833, 2334, 2381
EarI	CTCTTC	5 cut(s)	960, 1101, 1833, 2334, 2381
EciI	GGCGGA	1 cut(s)	276
EclXI	CGGCCG	2 cut(s)	76, 225
Eco130I	CCWWGG	7 cut(s)	34, 1404, 1773, 2055, 2174, 2190, 2200
Eco147I	AGGCCT	1 cut(s)	1403
Eco24I	GRGCYC	2 cut(s)	419, 1187
Eco31I	GGTCTC	1 cut(s)	135
Eco47I	GGWCC	1 cut(s)	1637
Eco47III	AGCGCT	1 cut(s)	1650
Eco52I	CGGCCG	2 cut(s)	76, 225
Eco57I	CTGAAG	3 cut(s)	909, 987, 1551
Eco72I	CACGTG	1 cut(s)	1916
EcoNI	CCTNNNNNAGG	2 cut(s)	1002, 1607
EcoO109I	RGGNCCY	3 cut(s)	416, 1184, 1627
EcoRI	GAATTC	2 cut(s)	2213, 2312
EcoRII	CCWGG	2 cut(s)	747, 1192
EcoT14I	CCWWGG	7 cut(s)	34, 1404, 1773, 2055, 2174, 2190, 2200
EcoT38I	GRGCYC	2 cut(s)	419, 1187
EgeI	GGCGCC	1 cut(s)	114
EheI	GGCGCC	1 cut(s)	114
ErhI	CCWWGG	7 cut(s)	34, 1404, 1773, 2055, 2174, 2190, 2200
Esp3I	CGTCTC	2 cut(s)	147, 315
FalI	AAGNNNNNCTT	6 cut(s)	805, 837, 1638, 1670, 2221, 2253
FaqI	GGGAC	3 cut(s)	1365, 1526, 2349
FauI	CCCGC	1 cut(s)	1406
FblI	GTMKAC	1 cut(s)	135
FokI	GGATG	9 cut(s)	494, 629, 785, 1184, 1421, 1491, 1957, 2024, 2075
FriOI	GRGCYC	2 cut(s)	419, 1187
FspBI	CTAG	6 cut(s)	1737, 1796, 2121, 2297, 2484, 2538
FspI	TGCGCA	1 cut(s)	1437
GlaI	GCGC	6 cut(s)	114, 1292, 1437, 1650, 1706, 2326
GsaI	CCCAGC	1 cut(s)	1352
HaeII	RGCGCY	3 cut(s)	116, 1652, 2328
HhaI	GCGC	6 cut(s)	115, 1293, 1438, 1651, 1707, 2327
Hin1I	GRCGYC	2 cut(s)	57, 113
Hin6I	GCGC	6 cut(s)	113, 1291, 1436, 1649, 1705, 2325
HinP1I	GCGC	6 cut(s)	113, 1291, 1436, 1649, 1705, 2325
HincII	GTYRAC	3 cut(s)	136, 2107, 2499
HindII	GTYRAC	3 cut(s)	136, 2107, 2499
HindIII	AAGCTT	2 cut(s)	47, 1341
HphI	GGTGA	4 cut(s)	230, 379, 763, 2030
Hpy166II	GTNNAC	8 cut(s)	136, 386, 773, 1022, 1742, 2107, 2352, 2499
Hpy8I	GTNNAC	8 cut(s)	136, 386, 773, 1022, 1742, 2107, 2352, 2499
Hpy99I	CGWCG	6 cut(s)	59, 62, 137, 140, 197, 2515
HpyAV	CCTTC	9 cut(s)	489, 1265, 1469, 1615, 1842, 1861, 1924, 2396, 2553
HpyCH4III	ACNGT	5 cut(s)	354, 742, 1096, 2104, 2286
HpyCH4IV	ACGT	6 cut(s)	57, 775, 1099, 1229, 1543, 1915
HpyF10VI	GCNNNNNNNGC	9 cut(s)	548, 650, 1297, 1340, 1562, 1967, 2186, 2414, 2417
HpyF3I	CTNAG	5 cut(s)	306, 845, 911, 1211, 1581
HpySE526I	ACGT	6 cut(s)	57, 775, 1099, 1229, 1543, 1915
Hsp92I	GRCGYC	2 cut(s)	57, 113
HspAI	GCGC	6 cut(s)	113, 1291, 1436, 1649, 1705, 2325
KasI	GGCGCC	1 cut(s)	112
Kzo9I	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
LguI	GCTCTTC	2 cut(s)	960, 2334
LmnI	GCTCC	5 cut(s)	62, 162, 1661, 2083, 2474
LweI	GCATC	9 cut(s)	472, 747, 1477, 1543, 1567, 1899, 1979, 2011, 2462
MaeI	CTAG	6 cut(s)	1737, 1796, 2121, 2297, 2484, 2538
MaeII	ACGT	6 cut(s)	57, 775, 1099, 1229, 1543, 1915
MaeIII	GTNAC	1 cut(s)	2518
MalI	GATC	9 cut(s)	6, 10, 237, 328, 1059, 1065, 1282, 1363, 1859
MboI	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
MfeI	CAATTG	2 cut(s)	1178, 2158
MflI	RGATCY	3 cut(s)	1057, 1280, 1361
MhlI	GDGCHC	6 cut(s)	419, 440, 646, 1024, 1187, 1744
Mly113I	GGCGCC	1 cut(s)	113
MlyI	GAGTC	5 cut(s)	252, 381, 1047, 1165, 1892
MmeI	TCCRAC	5 cut(s)	40, 213, 1351, 1522, 1989
MroXI	GAANNNNTTC	3 cut(s)	840, 855, 1697
MseI	TTAA	8 cut(s)	681, 924, 975, 1358, 1539, 2208, 2228, 2277
MslI	CAYNNNNRTG	3 cut(s)	683, 1918, 2378
MspA1I	CMGCKG	2 cut(s)	1721, 2414
MspCI	CTTAAG	2 cut(s)	1357, 2227
MspR9I	CCNGG	6 cut(s)	80, 229, 248, 749, 1194, 2334
MunI	CAATTG	2 cut(s)	1178, 2158
Mva1269I	GAATGC	2 cut(s)	626, 1334
MvaI	CCWGG	2 cut(s)	749, 1194
MvnI	CGCG	1 cut(s)	1707
MwoI	GCNNNNNNNGC	9 cut(s)	548, 650, 1297, 1340, 1562, 1967, 2186, 2414, 2417
NarI	GGCGCC	1 cut(s)	113
NciI	CCSGG	4 cut(s)	80, 229, 248, 2334
NdeII	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
NheI	GCTAGC	1 cut(s)	2483
NlaIV	GGNNCC	6 cut(s)	114, 417, 692, 1185, 1639, 2364
NmeAIII	GCCGAG	1 cut(s)	2285
NmuCI	GTSAC	1 cut(s)	2518
NsbI	TGCGCA	1 cut(s)	1437
NspI	RCATGY	3 cut(s)	688, 1130, 1325
NspV	TTCGAA	3 cut(s)	24, 127, 276
PaeI	GCATGC	1 cut(s)	1130
PagI	TCATGA	2 cut(s)	2217, 2373
PceI	AGGCCT	1 cut(s)	1403
PciI	ACATGT	2 cut(s)	684, 1321
PciSI	GCTCTTC	2 cut(s)	960, 2334
PctI	GAATGC	2 cut(s)	626, 1334
PdmI	GAANNNNTTC	3 cut(s)	840, 855, 1697
PfeI	GAWTC	8 cut(s)	129, 273, 345, 872, 1451, 1715, 1904, 2525
PfoI	TCCNGGA	1 cut(s)	2332
PinAI	ACCGGT	1 cut(s)	838
Ple19I	CGATCG	1 cut(s)	11
PleI	GAGTC	5 cut(s)	251, 381, 1046, 1164, 1892
PluTI	GGCGCC	1 cut(s)	116
PmaCI	CACGTG	1 cut(s)	1916
PmlI	CACGTG	1 cut(s)	1916
PpsI	GAGTC	5 cut(s)	251, 381, 1046, 1164, 1892
Ppu21I	YACGTR	3 cut(s)	776, 1230, 1916
PscI	ACATGT	2 cut(s)	684, 1321
PsiI	TTATAA	1 cut(s)	359
Psp6I	CCWGG	2 cut(s)	747, 1192
PspCI	CACGTG	1 cut(s)	1916
PspFI	CCCAGC	1 cut(s)	1348
PspGI	CCWGG	2 cut(s)	747, 1192
PspN4I	GGNNCC	6 cut(s)	114, 417, 692, 1185, 1639, 2364
PspOMI	GGGCCC	2 cut(s)	415, 1183
PspPI	GGNCC	6 cut(s)	415, 416, 1183, 1184, 1627, 1637
PstI	CTGCAG	3 cut(s)	1448, 2422, 2449
PstNI	CAGNNNCTG	4 cut(s)	1490, 2414, 2417, 2528
PsuI	RGATCY	3 cut(s)	1057, 1280, 1361
PvuI	CGATCG	1 cut(s)	11
PvuII	CAGCTG	2 cut(s)	1721, 2414
RsaI	GTAC	4 cut(s)	568, 592, 1098, 1369
RsaNI	GTAC	4 cut(s)	567, 591, 1097, 1368
RseI	CAYNNNNRTG	3 cut(s)	683, 1918, 2378
SalI	GTCGAC	1 cut(s)	134
SapI	GCTCTTC	2 cut(s)	960, 2334
SaqAI	TTAA	8 cut(s)	681, 924, 975, 1358, 1539, 2208, 2228, 2277
Sau3AI	GATC	9 cut(s)	4, 8, 235, 326, 1057, 1063, 1280, 1361, 1857
Sau96I	GGNCC	6 cut(s)	415, 416, 1183, 1184, 1627, 1637
SchI	GAGTC	5 cut(s)	252, 381, 1047, 1165, 1892
ScrFI	CCNGG	6 cut(s)	80, 229, 248, 749, 1194, 2334
SduI	GDGCHC	6 cut(s)	419, 440, 646, 1024, 1187, 1744
SfaNI	GCATC	9 cut(s)	472, 747, 1477, 1543, 1567, 1899, 1979, 2011, 2462
SfcI	CTRYAG	3 cut(s)	1444, 2418, 2445
SfoI	GGCGCC	1 cut(s)	114
SfuI	TTCGAA	3 cut(s)	24, 127, 276
SgrDI	CGTCGACG	1 cut(s)	134
SinI	GGWCC	1 cut(s)	1637
SmiMI	CAYNNNNRTG	3 cut(s)	683, 1918, 2378
SmlI	CTYRAG	2 cut(s)	1357, 2227
SmoI	CTYRAG	2 cut(s)	1357, 2227
SphI	GCATGC	1 cut(s)	1130
SseBI	AGGCCT	1 cut(s)	1403
SsiI	CCGC	6 cut(s)	102, 110, 116, 261, 1413, 2317
SspDI	GGCGCC	1 cut(s)	112
SspI	AATATT	1 cut(s)	2032
SspMI	CTAG	6 cut(s)	1737, 1796, 2121, 2297, 2484, 2538
StuI	AGGCCT	1 cut(s)	1403
StyD4I	CCNGG	6 cut(s)	78, 227, 246, 747, 1192, 2332
StyI	CCWWGG	7 cut(s)	34, 1404, 1773, 2055, 2174, 2190, 2200
TaaI	ACNGT	5 cut(s)	354, 742, 1096, 2104, 2286
TaiI	ACGT	6 cut(s)	60, 778, 1102, 1232, 1546, 1918
TatI	WGTACW	1 cut(s)	566
TauI	GCSGC	2 cut(s)	113, 118
TfiI	GAWTC	8 cut(s)	129, 273, 345, 872, 1451, 1715, 1904, 2525
Tru1I	TTAA	8 cut(s)	681, 924, 975, 1358, 1539, 2208, 2228, 2277
Tru9I	TTAA	8 cut(s)	681, 924, 975, 1358, 1539, 2208, 2228, 2277
TscAI	CASTG	4 cut(s)	299, 346, 1453, 2289
TseFI	GTSAC	1 cut(s)	2518
Tsp45I	GTSAC	1 cut(s)	2518
TspGWI	ACGGA	5 cut(s)	153, 165, 339, 791, 1850
TspRI	CASTG	4 cut(s)	299, 346, 1453, 2289
Vha464I	CTTAAG	2 cut(s)	1357, 2227
VneI	GTGCAC	2 cut(s)	1020, 1740
VpaK11BI	GGWCC	1 cut(s)	1637
XagI	CCTNNNNNAGG	2 cut(s)	1002, 1607
XapI	RAATTY	5 cut(s)	829, 917, 1782, 2213, 2312
XbaI	TCTAGA	1 cut(s)	1795
XceI	RCATGY	3 cut(s)	688, 1130, 1325
XmiI	GTMKAC	1 cut(s)	135
XmnI	GAANNNNTTC	3 cut(s)	840, 855, 1697
XspI	CTAG	6 cut(s)	1737, 1796, 2121, 2297, 2484, 2538
ZraI	GACGTC	1 cut(s)	58

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.