Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004898

Physical Location & Seq

Genomic Coordinates Forward (+)

60012975 .. 60022044

Gene Structure 9070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020013

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGGCAAAGAGTGAGCAGTGCTGCTCTACTCAGCTTATTGATGGAGACGGATCATTCAATGACACTGGAATCGAACAGTTTATAAAGGAGGTGAAATTGGGAGAATGTGGACTCTCCTATGCAGTAGTCTCCATTATGGGCCCTCAAAGTAGTGGAAAGAGCACACTACTAAATAATTTGTTTGCTACCAACTTCAAGGAGATGGATGCTTTTAGAGGAAGGTCTCAGACAACAAAAGGTATTTGGTTGGCAAAATGTGCTGGTATTGAGCCGTGTACTCTTGTAATGGATTTGGAGGGTACAGATGGGAGAGAACGAGGAGAGGATGATACGGCATTCGAGAAGCAGAGTGCCCTCTTTGCCCTTGCTGTTTCAGATATAGTTCTCATTAACATGTGGTGCCATGATATTGGCCGTGAGCAAGCCGCAAATAAGCCTCTCCTAAAGACTGTTTTCCAGGTGATGATGCGATTGTTTAGTCCACGTAAAACAACTCTGATGTTCGTCATCCGTGATAAGACAAGGACACCTTTGGAGAATTTAGAACCAGTTCTTAGAGAAGACATTCAGAAGATATGGGACTCTGTTCCGAAGCCTGAAGCTCACAAGGATACTCCACTAAGCGAATTTTTTAATGTCGAGGTTGTTGCTCTTTCAAGTTATGAAGAAAAAGAAGAGCAATTTAAAGAGCAGGTCGCTAGCTTGAGGCAGAAATTCTTTCATTCCATTGCCCCTGGTGGGCTTGCTGGAGATAGACGGGGGGTAGTTCCTGCTTCAGGGTTTTCTTTTAGTGCACAGCAGATATGGAGAGTCATCAAAGAAAACAGAGATCTTGATCTTCCTGCCCACAAGGTCATGGTGGCAACTGTACGTTGTGAAGAGATTGCCAACGAGAAGCATGCTGCTTTTGTAGGAAATGAGGAGTGGAATCAGTTTGAGCAGGACGTTCAATTGGGCCCTATTCCTGGCTTTGGGAAGAAGCTCAGTTCAATTATTGATACGTGTCTATCAGGGTATGATGAAGAAGCTACATACTTTGATGAAGGTGTCAGATCTGGGAAGCGCAAGCAGCTTGAAGAAAAATTGCTTCAACATGTCCAACCTGCATTCCAAGCTTTGCTGGGACACTTAAGATCTGGTACTCTGGATAAGTTCAAGGAAGCATTTGAAAAGGCCTTGGATGGCGGGCAGGGTTTTTCTGTGGCTGCGCATAATTGCAGTGAATCTTTCATGGCTCAATTTGATGAAGGATGTGCAGATGCTGTTATCCAACAAGCAGATTGGGACACATCTAAAGTAAGAGACAAACTTAAACGTGATATAGATGCTCACATTGCTTCAGTTCGTGATGCTAAGCTATCCGAAATTACGACCCTTTATGAGGCAAAATTGAAGGAGGCCTTATCTGGACCTGTGGAAGCGCTACTTGATGGAGCTAATAGTGAGACTTGGCCTGCAATAAGGAAACTTTTCAAGCGTGAGACTGAATCGGCTGTCTCTGGGTTCTCTAGTGCTCTTTCTGGTTTTGATATGGATAAACAAACCAAGGATAAAATTCTTGCAAGTCTAGAGGCATATTCAAGAGGTGTAGTGGAAGCAAAAACAAAGGAAGAGGCCGGAAGGGTTTTGATCCGTATGAAGGACAGATTTGCAACGCTATTTAGCCACGACTCTGATTCGATGCCACGTGTATGGACTGGGAAGGAAGACATCAGAGCAATTACCAAGACTGCTCGTTCTGCATCCCTGAAATTGCTTTCTGTTATGGCTGCAATTCGGTTGGATGATGGTGATGCTGATAATATTGAGAAAACTTTATCTCTTGCCTTGGTGGATGGCAGAAATGCTGCTGCCAAAGATAAGAGTATCACAACGGTTGACCCATTGGCCTCTAGCACTTGGCAAGAGGTTTCATCATCAAAGACATTGATCACACCTGTACAGTGCAAATCTTTGTGGAAGCAATTCAAGTCACAGACTGAGTACAGTGTTTCTCAGGCTATTGCTGCCCAGGAAGCCAACAAGCGTAATAACAATTGGCTACCACCTCCTTGGGCTATTCTTGCCTTGGTTGTCCTCGGATTTAATGAATTCATGACACTCTTAAGAAATCCTTTGTATCTGCTGGTCATCTTTGTTGGCTTTTTACTCCTTAAAGCACTATGGGTGCAGCTAGACATCTCGGCTGAATTCCGCAATGGCGCTCTTCCGGGACTTCTCTCTTTGTCCACCAAGTTGGTGCCAACTATCATGAACATGATGAAGAGATTAGCAGATGAAGGGGCAGCAGCTGCTGCAGATAACAATCCTCAAAGAAACCGTGCAGTAGCATCAAAAGGCATAAGCAATGGAGGAAATGCTAGCAGTTCCATGTCAACTACTGCCTCGTCGGGAGTGACGGAATCTGAATACTCTAGTCCTTCAAAACAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000902 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0006810 GO:0006888 GO:0006996 GO:0007010 GO:0007275 GO:0008150 GO:0009653 GO:0009826 GO:0009832 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0017076 GO:0019001 GO:0022622 GO:0030029 GO:0030036 GO:0030154 GO:0031090 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032989 GO:0035639 GO:0036094 GO:0040007 GO:0042546 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048364 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060560 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:0099402 GO:1901265 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RHD3

Protein Analysis

812

Amino Acids

89.39

Weight (kDa)

5.69

Isoelectric Point (pI)

37.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
URGCP_GTPase	PF25683	11 - 162	4.1e-08	URGCP-like GTPase domain
RHD3_GTPase	PF05879	46 - 281	3.4e-117	Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB	PF20428	323 - 760	7.9e-153	Sey1 three-helix bundle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13870 AT3G13870
fragaria_vesca	FvH4_6g33350 FvH4_6g33350 FvH4_6g33350 FvH4_6g33350
malus_domestica	MD09G1193400.v1.1 MD17G1174200.v1.1
prunus_persica	Prupe.3G049800_v2.0.a1
pyrus_communis	pycom09g10990 pycom17g17830
rosa_chinensis	RchiOBHm_Chr2g0143201 RchiOBHm_Chr2g0169871
rosa_laevigata	RLG00000020013
rosa_multiflora	Rmu_co8092052.1_g000001 Rmu_sc0000181.1_g000004 Rmu_sc0003950.1_g000012
rosa_roxburghii	Rroxscaffold_2G00102050 Rroxscaffold_5G00359600 Rroxscaffold_7G00190950 Rroxscaffold_7G00191100
rosa_rugosa	Rorug02G0379200
rosa_samantha	Rh1BG269400 Rh2AG432000 Rh2BG440600 Rh2CG385100 Rh2CG417900 Rh2DG450800 Rh7AG446900
rosa_wichuraiana	Rw2G035290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	83
Acc16I	TGCGCA	1 cut(s)	1209
Acc36I	ACCTGC	2 cut(s)	682, 1111
AccB1I	GGYRCC	2 cut(s)	399, 2239
AciI	CCGC	3 cut(s)	426, 1185, 2194
AclWI	GGATC	2 cut(s)	58, 1624
AcoI	YGGCCR	1 cut(s)	412
AcsI	RAATTY	6 cut(s)	538, 626, 713, 1554, 2090, 2189
AcuI	CTGAAG	3 cut(s)	618, 759, 1323
AcvI	CACGTG	1 cut(s)	1688
AfaI	GTAC	6 cut(s)	277, 301, 870, 1141, 1941, 1985
AfeI	AGCGCT	1 cut(s)	1422
AfiI	CCNNNNNNNGG	6 cut(s)	738, 776, 965, 971, 1381, 1639
AflII	CTTAAG	2 cut(s)	1129, 2104
AflIII	ACRYGT	4 cut(s)	393, 1001, 1093, 1687
AjnI	CCWGG	4 cut(s)	456, 733, 964, 2010
Alw21I	GWGCWC	3 cut(s)	164, 796, 1516
Alw26I	GTCTC	7 cut(s)	39, 133, 228, 1296, 1439, 1475, 1501
Alw44I	GTGCAC	1 cut(s)	792
AlwI	GGATC	2 cut(s)	58, 1624
AlwNI	CAGNNNCTG	3 cut(s)	1262, 2291, 2294
Aor51HI	AGCGCT	1 cut(s)	1422
AoxI	GGCC	8 cut(s)	139, 412, 955, 1173, 1398, 1451, 1614, 1887
ApaI	GGGCCC	2 cut(s)	143, 959
ApaLI	GTGCAC	1 cut(s)	792
ApoI	RAATTY	6 cut(s)	538, 626, 713, 1554, 2090, 2189
Asp700I	GAANNNNTTC	4 cut(s)	549, 564, 1469, 1964
AspLEI	GCGC	4 cut(s)	1065, 1210, 1423, 2204
AspS9I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
AsuC2I	CCSGG	1 cut(s)	2211
AsuHPI	GGTGA	3 cut(s)	103, 472, 1802
AsuNHI	GCTAGC	2 cut(s)	698, 2360
AvaII	GGWCC	1 cut(s)	1409
BaeGI	GKGCMC	4 cut(s)	143, 355, 796, 959
BanI	GGYRCC	2 cut(s)	399, 2239
BanII	GRGCYC	2 cut(s)	143, 959
BbrPI	CACGTG	1 cut(s)	1688
BbsI	GAAGAC	2 cut(s)	567, 1713
Bbv12I	GWGCWC	3 cut(s)	164, 796, 1516
BccI	CCATC	7 cut(s)	35, 196, 299, 1175, 1424, 1781, 1829
BceAI	ACGGC	3 cut(s)	256, 348, 399
BcgI	CGANNNNNNTGC	4 cut(s)	629, 663, 881, 915
BciT130I	CCWGG	4 cut(s)	458, 735, 966, 2012
BciVI	GTATCC	1 cut(s)	604
BclI	TGATCA	1 cut(s)	1929
BcnI	CCSGG	1 cut(s)	2211
BcoDI	GTCTC	7 cut(s)	39, 133, 228, 1296, 1439, 1475, 1501
BfaI	CTAG	7 cut(s)	699, 1509, 1568, 1893, 2174, 2361, 2415
BfmI	CTRYAG	1 cut(s)	2295
BfoI	RGCGCY	2 cut(s)	1424, 2205
BfrI	CTTAAG	2 cut(s)	1129, 2104
BfuAI	ACCTGC	2 cut(s)	682, 1111
BfuI	GTATCC	1 cut(s)	604
BglII	AGATCT	3 cut(s)	829, 1052, 1133
BlpI	GCTNAGC	1 cut(s)	1353
Bme1390I	CCNGG	5 cut(s)	458, 735, 966, 2012, 2211
Bme18I	GGWCC	1 cut(s)	1409
BmgT120I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
BmiI	GGNNCC	4 cut(s)	141, 401, 957, 2241
BmrFI	CCNGG	5 cut(s)	458, 735, 966, 2012, 2211
BmrI	ACTGGG	1 cut(s)	1707
BmsI	GCATC	9 cut(s)	196, 456, 1249, 1315, 1339, 1671, 1751, 1783, 2339
BmtI	GCTAGC	2 cut(s)	702, 2364
BmuI	ACTGGG	1 cut(s)	1707
BpiI	GAAGAC	2 cut(s)	567, 1713
BpmI	CTGGAG	1 cut(s)	768
Bpu1102I	GCTNAGC	1 cut(s)	1353
BpuEI	CTTGAG	1 cut(s)	724
BpuMI	CCSGG	1 cut(s)	2211
BsaAI	YACGTR	3 cut(s)	485, 1002, 1688
BsaBI	GATNNNNATC	2 cut(s)	834, 2304
BsaI	GGTCTC	1 cut(s)	228
BsaJI	CCNNGG	8 cut(s)	733, 1176, 1545, 1827, 2010, 2051, 2067, 2077
Bsc4I	CCNNNNNNNGG	6 cut(s)	738, 776, 965, 971, 1381, 1639
Bse1I	ACTGG	3 cut(s)	70, 548, 1702
Bse3DI	GCAATG	4 cut(s)	726, 1332, 2203, 2353
Bse8I	GATNNNNATC	2 cut(s)	834, 2304
BseBI	CCWGG	4 cut(s)	458, 735, 966, 2012
BseDI	CCNNGG	8 cut(s)	733, 1176, 1545, 1827, 2010, 2051, 2067, 2077
BseGI	GGATG	8 cut(s)	211, 331, 507, 1186, 1256, 1742, 1789, 1840
BseJI	GATNNNNATC	2 cut(s)	834, 2304
BseLI	CCNNNNNNNGG	6 cut(s)	738, 776, 965, 971, 1381, 1639
BseMI	GCAATG	4 cut(s)	726, 1332, 2203, 2353
BseMII	CTCAG	5 cut(s)	44, 239, 997, 1971, 2009
BseNI	ACTGG	3 cut(s)	70, 548, 1702
BseRI	GAGGAG	2 cut(s)	333, 935
BseSI	GKGCMC	4 cut(s)	143, 355, 796, 959
BseYI	CCCAGC	1 cut(s)	1120
BsgI	GTGCAG	3 cut(s)	1275, 2189, 2343
BshFI	GGCC	8 cut(s)	141, 414, 957, 1175, 1400, 1453, 1616, 1889
BshNI	GGYRCC	2 cut(s)	399, 2239
BsiHKAI	GWGCWC	3 cut(s)	164, 796, 1516
BsiSI	CCGG	2 cut(s)	1617, 2210
BslFI	GGGAC	4 cut(s)	593, 1137, 1298, 2226
BslI	CCNNNNNNNGG	6 cut(s)	738, 776, 965, 971, 1381, 1639
BsmAI	GTCTC	7 cut(s)	39, 133, 228, 1296, 1439, 1475, 1501
BsmBI	CGTCTC	1 cut(s)	39
BsmFI	GGGAC	4 cut(s)	593, 1137, 1298, 2226
BsmI	GAATGC	2 cut(s)	335, 1106
BsnI	GGCC	8 cut(s)	141, 414, 957, 1175, 1400, 1453, 1616, 1889
Bso31I	GGTCTC	1 cut(s)	228
Bsp120I	GGGCCC	2 cut(s)	139, 955
Bsp1286I	GDGCHC	6 cut(s)	143, 164, 355, 796, 959, 1516
Bsp1407I	TGTACA	1 cut(s)	1939
Bsp143I	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
Bsp1720I	GCTNAGC	1 cut(s)	1353
BspACI	CCGC	3 cut(s)	426, 1185, 2194
BspANI	GGCC	8 cut(s)	141, 414, 957, 1175, 1400, 1453, 1616, 1889
BspCNI	CTCAG	5 cut(s)	43, 238, 996, 1972, 2008
BspHI	TCATGA	2 cut(s)	2094, 2250
BspLI	GGNNCC	4 cut(s)	141, 401, 957, 2241
BspMAI	CTGCAG	1 cut(s)	2299
BspMI	ACCTGC	2 cut(s)	682, 1111
BspOI	GCTAGC	2 cut(s)	702, 2364
BspPI	GGATC	2 cut(s)	58, 1624
BspQI	GCTCTTC	2 cut(s)	669, 2211
BspT107I	GGYRCC	2 cut(s)	399, 2239
BspTI	CTTAAG	2 cut(s)	1129, 2104
BspTNI	GGTCTC	1 cut(s)	228
BsrDI	GCAATG	4 cut(s)	726, 1332, 2203, 2353
BsrGI	TGTACA	1 cut(s)	1939
BsrI	ACTGG	3 cut(s)	70, 548, 1702
BssECI	CCNNGG	8 cut(s)	733, 1176, 1545, 1827, 2010, 2051, 2067, 2077
BssMI	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
BssT1I	CCWWGG	5 cut(s)	1176, 1545, 1827, 2051, 2067
Bst2UI	CCWGG	4 cut(s)	458, 735, 966, 2012
Bst4CI	ACNGT	7 cut(s)	78, 451, 868, 1876, 1944, 1988, 2321
Bst6I	CTCTTC	5 cut(s)	669, 873, 1605, 2211, 2258
BstAFI	CTTAAG	2 cut(s)	1129, 2104
BstAPI	GCANNNNNTGC	3 cut(s)	257, 2291, 2294
BstAUI	TGTACA	1 cut(s)	1939
BstBAI	YACGTR	3 cut(s)	485, 1002, 1688
BstC8I	GCNNGC	8 cut(s)	423, 700, 744, 900, 1067, 1187, 1455, 2362
BstDEI	CTNAG	8 cut(s)	30, 225, 554, 620, 983, 1353, 1980, 1995
BstENI	CCTNNNNNAGG	2 cut(s)	774, 1379
BstF5I	GGATG	8 cut(s)	211, 331, 507, 1186, 1256, 1742, 1789, 1840
BstH2I	RGCGCY	2 cut(s)	1424, 2205
BstHHI	GCGC	4 cut(s)	1065, 1210, 1423, 2204
BstKTI	GATC	7 cut(s)	53, 832, 838, 1055, 1136, 1632, 1932
BstMAI	GTCTC	7 cut(s)	39, 133, 228, 1296, 1439, 1475, 1501
BstMBI	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
BstNI	CCWGG	4 cut(s)	458, 735, 966, 2012
BstNSI	RCATGY	3 cut(s)	397, 902, 1097
BstSCI	CCNGG	5 cut(s)	456, 733, 964, 2010, 2209
BstSFI	CTRYAG	1 cut(s)	2295
BstSLI	GKGCMC	4 cut(s)	143, 355, 796, 959
BstV2I	GAAGAC	2 cut(s)	567, 1713
BstX2I	RGATCY	3 cut(s)	829, 1052, 1133
BstXI	CCANNNNNNTGG	4 cut(s)	410, 1692, 2052, 2236
BstYI	RGATCY	3 cut(s)	829, 1052, 1133
BsuI	GTATCC	1 cut(s)	604
BsuRI	GGCC	8 cut(s)	141, 414, 957, 1175, 1400, 1453, 1616, 1889
BtsCI	GGATG	8 cut(s)	211, 331, 507, 1186, 1256, 1742, 1789, 1840
BtsI	GCAGTG	2 cut(s)	23, 1225
BtsIMutI	CAGTG	5 cut(s)	23, 63, 1225, 1949, 1993
BveI	ACCTGC	2 cut(s)	682, 1111
Cac8I	GCNNGC	8 cut(s)	423, 700, 744, 900, 1067, 1187, 1455, 2362
CaiI	CAGNNNCTG	3 cut(s)	1262, 2291, 2294
CciI	TCATGA	2 cut(s)	2094, 2250
CfoI	GCGC	4 cut(s)	1065, 1210, 1423, 2204
Cfr13I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
Csp6I	GTAC	6 cut(s)	276, 300, 869, 1140, 1940, 1984
CviQI	GTAC	6 cut(s)	276, 300, 869, 1140, 1940, 1984
DdeI	CTNAG	8 cut(s)	30, 225, 554, 620, 983, 1353, 1980, 1995
DpnI	GATC	7 cut(s)	52, 831, 837, 1054, 1135, 1631, 1931
DpnII	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
DraI	TTTAAA	1 cut(s)	685
EaeI	YGGCCR	1 cut(s)	412
Eam1104I	CTCTTC	5 cut(s)	669, 873, 1605, 2211, 2258
EarI	CTCTTC	5 cut(s)	669, 873, 1605, 2211, 2258
Eco130I	CCWWGG	5 cut(s)	1176, 1545, 1827, 2051, 2067
Eco147I	AGGCCT	2 cut(s)	1175, 1400
Eco24I	GRGCYC	2 cut(s)	143, 959
Eco31I	GGTCTC	1 cut(s)	228
Eco47I	GGWCC	1 cut(s)	1409
Eco47III	AGCGCT	1 cut(s)	1422
Eco57I	CTGAAG	3 cut(s)	618, 759, 1323
Eco72I	CACGTG	1 cut(s)	1688
EcoNI	CCTNNNNNAGG	2 cut(s)	774, 1379
EcoO109I	RGGNCCY	2 cut(s)	140, 956
EcoRI	GAATTC	2 cut(s)	2090, 2189
EcoRII	CCWGG	4 cut(s)	456, 733, 964, 2010
EcoT14I	CCWWGG	5 cut(s)	1176, 1545, 1827, 2051, 2067
EcoT38I	GRGCYC	2 cut(s)	143, 959
ErhI	CCWWGG	5 cut(s)	1176, 1545, 1827, 2051, 2067
Esp3I	CGTCTC	1 cut(s)	39
FalI	AAGNNNNNCTT	8 cut(s)	514, 546, 1385, 1417, 1410, 1442, 2098, 2130
FaqI	GGGAC	4 cut(s)	593, 1137, 1298, 2226
FauI	CCCGC	1 cut(s)	1178
FbaI	TGATCA	1 cut(s)	1929
FokI	GGATG	8 cut(s)	218, 338, 494, 1193, 1263, 1729, 1796, 1847
FriOI	GRGCYC	2 cut(s)	143, 959
FspBI	CTAG	7 cut(s)	699, 1509, 1568, 1893, 2174, 2361, 2415
FspI	TGCGCA	1 cut(s)	1209
GlaI	GCGC	4 cut(s)	1064, 1209, 1422, 2203
GsaI	CCCAGC	1 cut(s)	1124
GsuI	CTGGAG	1 cut(s)	768
HaeII	RGCGCY	2 cut(s)	1424, 2205
HaeIII	GGCC	8 cut(s)	141, 414, 957, 1175, 1400, 1453, 1616, 1889
HapII	CCGG	2 cut(s)	1617, 2210
HhaI	GCGC	4 cut(s)	1065, 1210, 1423, 2204
Hin6I	GCGC	4 cut(s)	1063, 1208, 1421, 2202
HinP1I	GCGC	4 cut(s)	1063, 1208, 1421, 2202
HincII	GTYRAC	2 cut(s)	1879, 2376
HindII	GTYRAC	2 cut(s)	1879, 2376
HindIII	AAGCTT	1 cut(s)	1113
HpaII	CCGG	2 cut(s)	1617, 2210
HphI	GGTGA	3 cut(s)	103, 472, 1802
Hpy166II	GTNNAC	7 cut(s)	110, 276, 482, 794, 1879, 2229, 2376
Hpy8I	GTNNAC	7 cut(s)	110, 276, 482, 794, 1879, 2229, 2376
Hpy99I	CGWCG	1 cut(s)	2392
HpyAV	CCTTC	9 cut(s)	213, 1037, 1241, 1387, 1614, 1633, 1696, 2273, 2430
HpyCH4III	ACNGT	7 cut(s)	78, 451, 868, 1876, 1944, 1988, 2321
HpyCH4IV	ACGT	6 cut(s)	484, 871, 945, 1001, 1315, 1687
HpyF3I	CTNAG	8 cut(s)	30, 225, 554, 620, 983, 1353, 1980, 1995
HpySE526I	ACGT	6 cut(s)	484, 871, 945, 1001, 1315, 1687
HspAI	GCGC	4 cut(s)	1063, 1208, 1421, 2202
Ksp22I	TGATCA	1 cut(s)	1929
Kzo9I	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
LguI	GCTCTTC	2 cut(s)	669, 2211
LmnI	GCTCC	1 cut(s)	1433
LweI	GCATC	9 cut(s)	196, 456, 1249, 1315, 1339, 1671, 1751, 1783, 2339
MaeI	CTAG	7 cut(s)	699, 1509, 1568, 1893, 2174, 2361, 2415
MaeII	ACGT	6 cut(s)	484, 871, 945, 1001, 1315, 1687
MaeIII	GTNAC	2 cut(s)	1971, 2395
MalI	GATC	7 cut(s)	52, 831, 837, 1054, 1135, 1631, 1931
MboI	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
MfeI	CAATTG	2 cut(s)	950, 2035
MflI	RGATCY	3 cut(s)	829, 1052, 1133
MhlI	GDGCHC	6 cut(s)	143, 164, 355, 796, 959, 1516
MlyI	GAGTC	4 cut(s)	105, 575, 819, 1664
MmeI	TCCRAC	3 cut(s)	1123, 1294, 1761
MroXI	GAANNNNTTC	4 cut(s)	549, 564, 1469, 1964
MseI	TTAA	8 cut(s)	390, 633, 684, 1130, 1311, 2085, 2105, 2154
MslI	CAYNNNNRTG	3 cut(s)	392, 1690, 2255
MspA1I	CMGCKG	1 cut(s)	2291
MspCI	CTTAAG	2 cut(s)	1129, 2104
MspI	CCGG	2 cut(s)	1617, 2210
MspR9I	CCNGG	5 cut(s)	458, 735, 966, 2012, 2211
MunI	CAATTG	2 cut(s)	950, 2035
Mva1269I	GAATGC	2 cut(s)	335, 1106
MvaI	CCWGG	4 cut(s)	458, 735, 966, 2012
NciI	CCSGG	1 cut(s)	2211
NdeII	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
NheI	GCTAGC	2 cut(s)	698, 2360
NlaIV	GGNNCC	4 cut(s)	141, 401, 957, 2241
NmeAIII	GCCGAG	1 cut(s)	2162
NmuCI	GTSAC	2 cut(s)	1971, 2395
NsbI	TGCGCA	1 cut(s)	1209
NspI	RCATGY	3 cut(s)	397, 902, 1097
PaeI	GCATGC	1 cut(s)	902
PagI	TCATGA	2 cut(s)	2094, 2250
PceI	AGGCCT	2 cut(s)	1175, 1400
PciI	ACATGT	2 cut(s)	393, 1093
PciSI	GCTCTTC	2 cut(s)	669, 2211
PctI	GAATGC	2 cut(s)	335, 1106
PdmI	GAANNNNTTC	4 cut(s)	549, 564, 1469, 1964
PfeI	GAWTC	6 cut(s)	69, 928, 1223, 1487, 1676, 2402
PfoI	TCCNGGA	1 cut(s)	2209
PleI	GAGTC	4 cut(s)	105, 575, 818, 1664
PmaCI	CACGTG	1 cut(s)	1688
PmlI	CACGTG	1 cut(s)	1688
PpsI	GAGTC	4 cut(s)	105, 575, 818, 1664
Ppu21I	YACGTR	3 cut(s)	485, 1002, 1688
PscI	ACATGT	2 cut(s)	393, 1093
PsiI	TTATAA	1 cut(s)	83
Psp6I	CCWGG	4 cut(s)	456, 733, 964, 2010
PspCI	CACGTG	1 cut(s)	1688
PspFI	CCCAGC	1 cut(s)	1120
PspGI	CCWGG	4 cut(s)	456, 733, 964, 2010
PspN4I	GGNNCC	4 cut(s)	141, 401, 957, 2241
PspOMI	GGGCCC	2 cut(s)	139, 955
PspPI	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
PstI	CTGCAG	1 cut(s)	2299
PstNI	CAGNNNCTG	3 cut(s)	1262, 2291, 2294
PsuI	RGATCY	3 cut(s)	829, 1052, 1133
PvuII	CAGCTG	1 cut(s)	2291
RsaI	GTAC	6 cut(s)	277, 301, 870, 1141, 1941, 1985
RsaNI	GTAC	6 cut(s)	276, 300, 869, 1140, 1940, 1984
RseI	CAYNNNNRTG	3 cut(s)	392, 1690, 2255
SapI	GCTCTTC	2 cut(s)	669, 2211
SaqAI	TTAA	8 cut(s)	390, 633, 684, 1130, 1311, 2085, 2105, 2154
Sau3AI	GATC	7 cut(s)	50, 829, 835, 1052, 1133, 1629, 1929
Sau96I	GGNCC	5 cut(s)	139, 140, 955, 956, 1409
SchI	GAGTC	4 cut(s)	105, 575, 819, 1664
ScrFI	CCNGG	5 cut(s)	458, 735, 966, 2012, 2211
SduI	GDGCHC	6 cut(s)	143, 164, 355, 796, 959, 1516
SfaNI	GCATC	9 cut(s)	196, 456, 1249, 1315, 1339, 1671, 1751, 1783, 2339
SfcI	CTRYAG	1 cut(s)	2295
SinI	GGWCC	1 cut(s)	1409
SmiMI	CAYNNNNRTG	3 cut(s)	392, 1690, 2255
SmlI	CTYRAG	3 cut(s)	703, 1129, 2104
SmoI	CTYRAG	3 cut(s)	703, 1129, 2104
SphI	GCATGC	1 cut(s)	902
SseBI	AGGCCT	2 cut(s)	1175, 1400
SsiI	CCGC	3 cut(s)	426, 1185, 2194
SspI	AATATT	1 cut(s)	1804
SspMI	CTAG	7 cut(s)	699, 1509, 1568, 1893, 2174, 2361, 2415
StuI	AGGCCT	2 cut(s)	1175, 1400
StyD4I	CCNGG	5 cut(s)	456, 733, 964, 2010, 2209
StyI	CCWWGG	5 cut(s)	1176, 1545, 1827, 2051, 2067
TaaI	ACNGT	7 cut(s)	78, 451, 868, 1876, 1944, 1988, 2321
TaiI	ACGT	6 cut(s)	487, 874, 948, 1004, 1318, 1690
TaqI	TCGA	4 cut(s)	72, 339, 639, 1679
TatI	WGTACW	3 cut(s)	275, 1939, 1983
TauI	GCSGC	1 cut(s)	428
TfiI	GAWTC	6 cut(s)	69, 928, 1223, 1487, 1676, 2402
Tru1I	TTAA	8 cut(s)	390, 633, 684, 1130, 1311, 2085, 2105, 2154
Tru9I	TTAA	8 cut(s)	390, 633, 684, 1130, 1311, 2085, 2105, 2154
TscAI	CASTG	5 cut(s)	23, 70, 1225, 1949, 1993
TseFI	GTSAC	2 cut(s)	1971, 2395
Tsp45I	GTSAC	2 cut(s)	1971, 2395
TspGWI	ACGGA	4 cut(s)	63, 500, 1622, 2414
TspRI	CASTG	5 cut(s)	23, 70, 1225, 1949, 1993
Vha464I	CTTAAG	2 cut(s)	1129, 2104
VneI	GTGCAC	1 cut(s)	792
VpaK11BI	GGWCC	1 cut(s)	1409
XagI	CCTNNNNNAGG	2 cut(s)	774, 1379
XapI	RAATTY	6 cut(s)	538, 626, 713, 1554, 2090, 2189
XbaI	TCTAGA	1 cut(s)	1567
XceI	RCATGY	3 cut(s)	397, 902, 1097
XmnI	GAANNNNTTC	4 cut(s)	549, 564, 1469, 1964
XspI	CTAG	7 cut(s)	699, 1509, 1568, 1893, 2174, 2361, 2415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.