Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004898

Physical Location & Seq

Genomic Coordinates Reverse (-)

19251060 .. 19265976

Gene Structure 14917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1174200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGGCAAAAAGTGAGGAGTGCTGTTCTACTCAGCTTATCGATGGGGATGGTACATTCAATGTCAGCGGAATTGACCGGTTTATCAAGGAGGCAAAATTGCCTGAATGCGGGCTTTCTTATGCGGTTGTCTCCATTATGGGCCCACAAAGTAGTGGAAAGAGCACATTGTTAAATCATTTATTTGCTACCAACTTTCGCGAGATGGATGCTTTTAGAGGAAGGTCACAAACAACTAAAGGTATTTGGGCTGCAAAATGCGCTGGCATTGAGCCTTGCACTCTTGTAATGGATTTAGAGGGTACCGATGGAAGAGAACGAGGAGAGGATGATACTGCATTCGAGAAGCAGAGTGCCCTCTTTGCTCTTGCTGTTTCAGATATAGTGCTCATAAACATGTGGTGCCATGATATTGGCCGTGAGCAAGCTGCAAACAAGCCTCTCCTGAAGACTGTGTTCCAAGTGATGATGCGATTATTTAGTCCACGTAAAACAACTCTGATGTTTGTCATCCGTGATAAAACAAGGACACCATTGGAAAATTTAGAACCTGTTCTTAGAGAAGACATTCAGAAGATATGGGACTCTGTTCCTAAGCCTGAAGCCCACAGGGAAACTCCACTAAGTGAATTTTTTAATGTTGAGGTTGTTGCTCTTTCAAGTTATGAAGAAAAAGAAGAGCAATTTAAGGAGCAGGTTGCTAGCTTGAGACAGAGATTTTATCATTCTATTGCGCCCGGTGGTCTTGCTGGAGACCGACGGGGGGTAGTTCCTGCTTCAGGGTTTTCGTTTAGTGCACAACAGATATGGAAAGTCATCAAAGAAAACAAAGATCTTGATCTTCCAGCCCACAAGGTCATGGTGGCTACTGTACGTTGTGAAGAAATTGCAAATGAGAAGTATGCTGCTTTTGCAGGAAATGAGGAGTGGAATGAGTTGGATAAGGCTGTTCAGTCTGGTCCTATTTCTGGCTTTGGGAAGAAGCTCAATTCAATCCTTGACACTTGTCTATCAGAGTATGATGCAGAGGCTACGTATTTTGATGAAGGTGTTAGAACTGGGAAGCGCAAGCAGCTTGAAGAAAAATTGCTGCAACTTGTACAACCTGCGTTCCAAGCTTTGCTGGGAATTATAAGATCTGGTACTCTGGATAAGTTCAAGGAAGCATTTGATAAGGCTTTGAGTGGTGGGGAGGGATTTTCAGCGGCCGCTCATAATTGCTCCGAGTCCTTTATGGCTCGGTTTGACAAAGGATGTTCAGATGCTGCTATCACACTAGCAAACTGGGACACATCTAAAGTAAGGGATAAGCTTAAACGTGATATAGAAGCCCATATTGCTTCAGTTCATGCTGCTAAGCTATCTGAACTTACTTCACTTTATGAGGGAAAGTTGAAGGATGCATTGTCAGCACCAGCAGAAGCTCTTCTAGATGGTGCTAATAGTGAGACCTGGCCCTCGATAAGAAAACTTTTCCGGCATGAGACAGAATCAGCTGTCTCCGGGCTCTCTAGTGCACTTTCCGGTTTTGATATGGATGAACAAGCTAAGGGCCAAATACTTGCAAATCTAGAGGCATATGCAAGAGGTGTGGTAGAAGCGAAAACAAAGGAAGAGGCTGGAAGGGTTTTGATTCGTATGAAGGACAGGTTTACGACATTATTTAGCCATGATTCTGATTCAATGCCGCGTGTGTGGACTGGGAAGGAGGATATTAGAGCAATTACCAAAACCGCTCGTTCTGCTTCTTTAAAATTGCTTTCTGTTATGGCCGCAATCCGTTTGGATGACGATGACGTTGATAATATTGAGAATACTTTGTCCCTTGCCTTGGTGGATTCCACAAATGCTGCTGTTAAAGATAGGAGTATCACAGCAGCTGATCCACTGGCTTCAAGCACTTGGCAAGAGATTTCAGCATCAAAAACATTGATCACACCTGTCCAGTGCAAATCTTTGTGGAGGCAATTCAAAGCAGAGACCGAGTACAGTGTTTCTCAGGCTATTTCTGCACAGGAAGCTAACAAGCGTAATAACAACTGGCTGCCACCTCCATGGGCAATTGTTGCCTTGATAATCCTGGGATTCAATGAATTTATGACACTTTTAAGAAATCCTTTGTATCTGTGCGTCATCTTTGTGGGATTTTTACTCGTTAAAGCCCTATGGGTGCAGCTAGACATTGCGGGTGAATTCCGCAATGGTGCTCTTCCTGGGCTTCTATGCTTGTCGACCAAGTTGGTTCCAACTATCATGAACATGATGAAAAGACTAGCAGAAGAAGGGGCAAAGCCTGCAGCTAATGATCCTCCGAGGAACCCTGCACTAGCATCAACAGGCTATAGGAATGAAGGGAATTCTAGCGGTGAAATGTCATCGGAAGTGACAGAATCTGAATGCTCCAGCCCCTCAAAACATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000902 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0006810 GO:0006888 GO:0006996 GO:0007010 GO:0007275 GO:0008150 GO:0009653 GO:0009826 GO:0009832 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0017076 GO:0019001 GO:0022622 GO:0030029 GO:0030036 GO:0030154 GO:0031090 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032989 GO:0035639 GO:0036094 GO:0040007 GO:0042546 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048364 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060560 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:0099402 GO:1901265 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RHD3

Protein Analysis

807

Amino Acids

88.85

Weight (kDa)

5.56

Isoelectric Point (pI)

39.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
URGCP_GTPase	PF25683	39 - 169	4.8e-07	URGCP-like GTPase domain
RHD3_GTPase	PF05879	46 - 281	3.6e-117	Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB	PF20428	323 - 760	1.1e-143	Sey1 three-helix bundle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004898)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13870 AT3G13870
fragaria_vesca	FvH4_6g33350 FvH4_6g33350 FvH4_6g33350 FvH4_6g33350
malus_domestica	MD09G1193400.v1.1 MD17G1174200.v1.1
prunus_persica	Prupe.3G049800_v2.0.a1
pyrus_communis	pycom09g10990 pycom17g17830
rosa_chinensis	RchiOBHm_Chr2g0143201 RchiOBHm_Chr2g0169871
rosa_laevigata	RLG00000020013
rosa_multiflora	Rmu_co8092052.1_g000001 Rmu_sc0000181.1_g000004 Rmu_sc0003950.1_g000012
rosa_roxburghii	Rroxscaffold_2G00102050 Rroxscaffold_5G00359600 Rroxscaffold_7G00190950 Rroxscaffold_7G00191100
rosa_rugosa	Rorug02G0379200
rosa_samantha	Rh1BG269400 Rh2AG432000 Rh2BG440600 Rh2CG385100 Rh2CG417900 Rh2DG450800 Rh7AG446900
rosa_wichuraiana	Rw2G035290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1130
Acc36I	ACCTGC	2 cut(s)	682, 1111
Acc65I	GGTACC	1 cut(s)	299
AccB1I	GGYRCC	2 cut(s)	299, 399
AccBSI	CCGCTC	2 cut(s)	1208, 1733
AccI	GTMKAC	1 cut(s)	2228
AccII	CGCG	2 cut(s)	198, 1687
AclWI	GGATC	2 cut(s)	1874, 2297
AcoI	YGGCCR	3 cut(s)	412, 1203, 1767
AcsI	RAATTY	5 cut(s)	538, 626, 2090, 2189, 2353
AcuI	CTGAAG	4 cut(s)	464, 618, 759, 1323
AdeI	CACNNNGTG	1 cut(s)	623
AfaI	GTAC	6 cut(s)	52, 301, 870, 1098, 1141, 1985
AfiI	CCNNNNNNNGG	5 cut(s)	107, 760, 776, 965, 1828
AflIII	ACRYGT	1 cut(s)	393
AgeI	ACCGGT	1 cut(s)	75
AjnI	CCWGG	3 cut(s)	1448, 2076, 2209
Alw21I	GWGCWC	5 cut(s)	164, 387, 796, 1516, 2206
Alw26I	GTCTC	7 cut(s)	133, 700, 744, 1439, 1475, 1501, 1970
Alw44I	GTGCAC	2 cut(s)	792, 1512
AlwI	GGATC	2 cut(s)	1874, 2297
AlwNI	CAGNNNCTG	3 cut(s)	1262, 1877, 2390
AoxI	GGCC	6 cut(s)	139, 412, 1203, 1451, 1549, 1767
ApaI	GGGCCC	1 cut(s)	143
ApaLI	GTGCAC	2 cut(s)	792, 1512
ApoI	RAATTY	5 cut(s)	538, 626, 2090, 2189, 2353
AsiGI	ACCGGT	1 cut(s)	75
Asp700I	GAANNNNTTC	3 cut(s)	549, 564, 1422
Asp718I	GGTACC	1 cut(s)	299
AspLEI	GCGC	3 cut(s)	260, 733, 1065
AspS9I	GGNCC	5 cut(s)	139, 140, 956, 1452, 1549
AsuC2I	CCSGG	2 cut(s)	735, 1501
AsuHPI	GGTGA	2 cut(s)	2198, 2375
AsuNHI	GCTAGC	1 cut(s)	698
AvaII	GGWCC	1 cut(s)	956
BaeGI	GKGCMC	4 cut(s)	143, 355, 796, 1516
BanI	GGYRCC	2 cut(s)	299, 399
BanII	GRGCYC	2 cut(s)	143, 1506
BbsI	GAAGAC	2 cut(s)	452, 567
Bbv12I	GWGCWC	5 cut(s)	164, 387, 796, 1516, 2206
BccI	CCATC	5 cut(s)	35, 41, 196, 299, 1424
BceAI	ACGGC	1 cut(s)	399
BcgI	CGANNNNNNTGC	2 cut(s)	188, 222
BciT130I	CCWGG	3 cut(s)	1450, 2078, 2211
BclI	TGATCA	1 cut(s)	1929
BcnI	CCSGG	2 cut(s)	735, 1501
BcoDI	GTCTC	7 cut(s)	133, 700, 744, 1439, 1475, 1501, 1970
BfmI	CTRYAG	2 cut(s)	2292, 2338
BfuAI	ACCTGC	2 cut(s)	682, 1111
BglII	AGATCT	2 cut(s)	829, 1133
BlpI	GCTNAGC	1 cut(s)	1353
Bme1390I	CCNGG	5 cut(s)	735, 1450, 1501, 2078, 2211
Bme18I	GGWCC	1 cut(s)	956
BmgT120I	GGNCC	5 cut(s)	139, 140, 956, 1452, 1549
BmiI	GGNNCC	5 cut(s)	141, 301, 401, 2241, 2315
BmrFI	CCNGG	5 cut(s)	735, 1450, 1501, 2078, 2211
BmrI	ACTGGG	3 cut(s)	1065, 1291, 1707
BmsI	GCATC	7 cut(s)	196, 456, 1009, 1249, 1387, 1925, 2336
BmtI	GCTAGC	1 cut(s)	702
BmuI	ACTGGG	3 cut(s)	1065, 1291, 1707
BoxI	GACNNNNGTC	1 cut(s)	1002
BpiI	GAAGAC	2 cut(s)	452, 567
BpmI	CTGGAG	2 cut(s)	768, 2383
Bpu10I	CCTNAGC	2 cut(s)	591, 1545
Bpu1102I	GCTNAGC	1 cut(s)	1353
BpuEI	CTTGAG	1 cut(s)	724
BpuMI	CCSGG	2 cut(s)	735, 1501
Bsa29I	ATCGAT	1 cut(s)	39
BsaAI	YACGTR	2 cut(s)	485, 1032
BsaBI	GATNNNNATC	1 cut(s)	834
BsaI	GGTCTC	3 cut(s)	744, 1439, 1970
BsaJI	CCNNGG	5 cut(s)	1827, 2051, 2077, 2210, 2309
BsaWI	WCCGGW	2 cut(s)	75, 1520
Bsc4I	CCNNNNNNNGG	5 cut(s)	107, 760, 776, 965, 1828
Bse118I	RCCGGY	1 cut(s)	75
Bse1I	ACTGG	6 cut(s)	1060, 1286, 1702, 1890, 1942, 2042
Bse3DI	GCAATG	2 cut(s)	2178, 2203
Bse8I	GATNNNNATC	1 cut(s)	834
BseBI	CCWGG	3 cut(s)	1450, 2078, 2211
BseCI	ATCGAT	1 cut(s)	39
BseDI	CCNNGG	5 cut(s)	1827, 2051, 2077, 2210, 2309
BseGI	GGATG	8 cut(s)	52, 211, 331, 507, 1256, 1402, 1540, 1789
BseJI	GATNNNNATC	1 cut(s)	834
BseLI	CCNNNNNNNGG	5 cut(s)	107, 760, 776, 965, 1828
BseMI	GCAATG	2 cut(s)	2178, 2203
BseMII	CTCAG	2 cut(s)	44, 2009
BseNI	ACTGG	6 cut(s)	1060, 1286, 1702, 1890, 1942, 2042
BseRI	GAGGAG	3 cut(s)	29, 333, 935
BseSI	GKGCMC	4 cut(s)	143, 355, 796, 1516
BseX3I	CGGCCG	1 cut(s)	1203
BseYI	CCCAGC	1 cut(s)	1120
BsgI	GTGCAG	3 cut(s)	1992, 2189, 2304
Bsh1236I	CGCG	2 cut(s)	198, 1687
Bsh1285I	CGRYCG	1 cut(s)	1206
BshFI	GGCC	6 cut(s)	141, 414, 1205, 1453, 1551, 1769
BshNI	GGYRCC	2 cut(s)	299, 399
BshTI	ACCGGT	1 cut(s)	75
BshVI	ATCGAT	1 cut(s)	39
BsiEI	CGRYCG	1 cut(s)	1206
BsiHKAI	GWGCWC	5 cut(s)	164, 387, 796, 1516, 2206
BsiSI	CCGG	5 cut(s)	76, 735, 1474, 1500, 1521
BslFI	GGGAC	3 cut(s)	593, 1298, 1804
BslI	CCNNNNNNNGG	5 cut(s)	107, 760, 776, 965, 1828
BsmAI	GTCTC	7 cut(s)	133, 700, 744, 1439, 1475, 1501, 1970
BsmFI	GGGAC	3 cut(s)	593, 1298, 1804
BsmI	GAATGC	3 cut(s)	110, 335, 2399
BsnI	GGCC	6 cut(s)	141, 414, 1205, 1453, 1551, 1769
Bso31I	GGTCTC	3 cut(s)	744, 1439, 1970
Bsp120I	GGGCCC	1 cut(s)	139
Bsp1286I	GDGCHC	8 cut(s)	143, 164, 355, 387, 796, 1506, 1516, 2206
Bsp1407I	TGTACA	1 cut(s)	1096
Bsp143I	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
Bsp1720I	GCTNAGC	1 cut(s)	1353
Bsp19I	CCATGG	1 cut(s)	2051
Bsp68I	TCGCGA	1 cut(s)	198
BspANI	GGCC	6 cut(s)	141, 414, 1205, 1453, 1551, 1769
BspCNI	CTCAG	2 cut(s)	43, 2008
BspDI	ATCGAT	1 cut(s)	39
BspFNI	CGCG	2 cut(s)	198, 1687
BspHI	TCATGA	1 cut(s)	2250
BspLI	GGNNCC	5 cut(s)	141, 301, 401, 2241, 2315
BspMAI	CTGCAG	1 cut(s)	2296
BspMI	ACCTGC	2 cut(s)	682, 1111
BspOI	GCTAGC	1 cut(s)	702
BspPI	GGATC	2 cut(s)	1874, 2297
BspQI	GCTCTTC	3 cut(s)	669, 1428, 2211
BspT107I	GGYRCC	2 cut(s)	299, 399
BspTNI	GGTCTC	3 cut(s)	744, 1439, 1970
BsrBI	CCGCTC	2 cut(s)	1208, 1733
BsrDI	GCAATG	2 cut(s)	2178, 2203
BsrFI	RCCGGY	1 cut(s)	75
BsrGI	TGTACA	1 cut(s)	1096
BsrI	ACTGG	6 cut(s)	1060, 1286, 1702, 1890, 1942, 2042
BssAI	RCCGGY	1 cut(s)	75
BssECI	CCNNGG	5 cut(s)	1827, 2051, 2077, 2210, 2309
BssMI	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
BssT1I	CCWWGG	2 cut(s)	1827, 2051
Bst2UI	CCWGG	3 cut(s)	1450, 2078, 2211
Bst4CI	ACNGT	3 cut(s)	451, 868, 1988
Bst6I	CTCTTC	5 cut(s)	304, 669, 1428, 1605, 2211
BstAPI	GCANNNNNTGC	2 cut(s)	2063, 2291
BstAUI	TGTACA	1 cut(s)	1096
BstBAI	YACGTR	2 cut(s)	485, 1032
BstC8I	GCNNGC	6 cut(s)	110, 262, 423, 700, 1067, 2292
BstDEI	CTNAG	7 cut(s)	30, 554, 591, 620, 1353, 1545, 1995
BstDSI	CCRYGG	1 cut(s)	2051
BstENI	CCTNNNNNAGG	1 cut(s)	774
BstF5I	GGATG	8 cut(s)	52, 211, 331, 507, 1256, 1402, 1540, 1789
BstFNI	CGCG	2 cut(s)	198, 1687
BstHHI	GCGC	3 cut(s)	260, 733, 1065
BstKTI	GATC	6 cut(s)	832, 838, 1136, 1882, 1932, 2305
BstMAI	GTCTC	7 cut(s)	133, 700, 744, 1439, 1475, 1501, 1970
BstMBI	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
BstMCI	CGRYCG	1 cut(s)	1206
BstNI	CCWGG	3 cut(s)	1450, 2078, 2211
BstNSI	RCATGY	1 cut(s)	397
BstPAI	GACNNNNGTC	1 cut(s)	1002
BstSCI	CCNGG	5 cut(s)	733, 1448, 1499, 2076, 2209
BstSFI	CTRYAG	2 cut(s)	2292, 2338
BstSLI	GKGCMC	4 cut(s)	143, 355, 796, 1516
BstSNI	TACGTA	1 cut(s)	1032
BstUI	CGCG	2 cut(s)	198, 1687
BstV2I	GAAGAC	2 cut(s)	452, 567
BstX2I	RGATCY	2 cut(s)	829, 1133
BstXI	CCANNNNNNTGG	2 cut(s)	410, 2052
BstYI	RGATCY	2 cut(s)	829, 1133
BstZI	CGGCCG	1 cut(s)	1203
Bsu15I	ATCGAT	1 cut(s)	39
BsuRI	GGCC	6 cut(s)	141, 414, 1205, 1453, 1551, 1769
BsuTUI	ATCGAT	1 cut(s)	39
BtgI	CCRYGG	1 cut(s)	2051
BtsCI	GGATG	8 cut(s)	52, 211, 331, 507, 1256, 1402, 1540, 1789
BtsIMutI	CAGTG	3 cut(s)	1883, 1949, 1993
BtuMI	TCGCGA	1 cut(s)	198
BveI	ACCTGC	2 cut(s)	682, 1111
Cac8I	GCNNGC	6 cut(s)	110, 262, 423, 700, 1067, 2292
CaiI	CAGNNNCTG	3 cut(s)	1262, 1877, 2390
CciI	TCATGA	1 cut(s)	2250
CciNI	GCGGCCGC	1 cut(s)	1203
CfoI	GCGC	3 cut(s)	260, 733, 1065
Cfr10I	RCCGGY	1 cut(s)	75
Cfr13I	GGNCC	5 cut(s)	139, 140, 956, 1452, 1549
ClaI	ATCGAT	1 cut(s)	39
CseI	GACGC	1 cut(s)	2116
Csp6I	GTAC	6 cut(s)	51, 300, 869, 1097, 1140, 1984
CspAI	ACCGGT	1 cut(s)	75
CspCI	CAANNNNNGTGG	2 cut(s)	1569, 1604
CviAII	CATG	9 cut(s)	394, 404, 856, 1346, 1478, 1667, 2052, 2251, 2257
CviQI	GTAC	6 cut(s)	51, 300, 869, 1097, 1140, 1984
DdeI	CTNAG	7 cut(s)	30, 554, 591, 620, 1353, 1545, 1995
DpnI	GATC	6 cut(s)	831, 837, 1135, 1881, 1931, 2304
DpnII	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
DraI	TTTAAA	1 cut(s)	1749
DraIII	CACNNNGTG	1 cut(s)	623
EaeI	YGGCCR	3 cut(s)	412, 1203, 1767
EagI	CGGCCG	1 cut(s)	1203
Eam1104I	CTCTTC	5 cut(s)	304, 669, 1428, 1605, 2211
EarI	CTCTTC	5 cut(s)	304, 669, 1428, 1605, 2211
EclXI	CGGCCG	1 cut(s)	1203
Eco105I	TACGTA	1 cut(s)	1032
Eco130I	CCWWGG	2 cut(s)	1827, 2051
Eco24I	GRGCYC	2 cut(s)	143, 1506
Eco31I	GGTCTC	3 cut(s)	744, 1439, 1970
Eco47I	GGWCC	1 cut(s)	956
Eco52I	CGGCCG	1 cut(s)	1203
Eco57I	CTGAAG	4 cut(s)	464, 618, 759, 1323
EcoNI	CCTNNNNNAGG	1 cut(s)	774
EcoRI	GAATTC	2 cut(s)	2189, 2353
EcoRII	CCWGG	3 cut(s)	1448, 2076, 2209
EcoT14I	CCWWGG	2 cut(s)	1827, 2051
EcoT22I	ATGCAT	1 cut(s)	1402
EcoT38I	GRGCYC	2 cut(s)	143, 1506
ErhI	CCWWGG	2 cut(s)	1827, 2051
FaeI	CATG	9 cut(s)	397, 407, 859, 1349, 1481, 1670, 2055, 2254, 2260
FalI	AAGNNNNNCTT	2 cut(s)	2098, 2130
FaqI	GGGAC	3 cut(s)	593, 1298, 1804
FatI	CATG	9 cut(s)	393, 403, 855, 1345, 1477, 1666, 2051, 2250, 2256
FauI	CCCGC	2 cut(s)	101, 2176
FauNDI	CATATG	1 cut(s)	1576
FbaI	TGATCA	1 cut(s)	1929
FblI	GTMKAC	1 cut(s)	2228
FokI	GGATG	8 cut(s)	59, 218, 338, 494, 1263, 1409, 1547, 1796
FriOI	GRGCYC	2 cut(s)	143, 1506
GlaI	GCGC	3 cut(s)	259, 732, 1064
GsaI	CCCAGC	1 cut(s)	1124
GsuI	CTGGAG	2 cut(s)	768, 2383
HaeIII	GGCC	6 cut(s)	141, 414, 1205, 1453, 1551, 1769
HapII	CCGG	5 cut(s)	76, 735, 1474, 1500, 1521
HgaI	GACGC	1 cut(s)	2116
HhaI	GCGC	3 cut(s)	260, 733, 1065
Hin1II	CATG	9 cut(s)	397, 407, 859, 1349, 1481, 1670, 2055, 2254, 2260
Hin6I	GCGC	3 cut(s)	258, 731, 1063
HinP1I	GCGC	3 cut(s)	258, 731, 1063
HincII	GTYRAC	1 cut(s)	2229
HindII	GTYRAC	1 cut(s)	2229
HindIII	AAGCTT	2 cut(s)	1113, 1307
HinfI	GANTC	9 cut(s)	581, 1223, 1487, 1630, 1670, 1676, 1835, 2082, 2387
HpaII	CCGG	5 cut(s)	76, 735, 1474, 1500, 1521
HphI	GGTGA	2 cut(s)	2198, 2375
Hpy166II	GTNNAC	6 cut(s)	482, 794, 1514, 1650, 1695, 2229
Hpy188III	TCNNGA	8 cut(s)	197, 340, 442, 833, 1145, 1427, 1568, 2251
Hpy8I	GTNNAC	6 cut(s)	482, 794, 1514, 1650, 1695, 2229
Hpy99I	CGWCG	1 cut(s)	759
HpyAV	CCTTC	8 cut(s)	213, 1037, 1387, 1614, 1633, 1696, 2273, 2342
HpyCH4III	ACNGT	3 cut(s)	451, 868, 1988
HpyCH4IV	ACGT	5 cut(s)	484, 871, 1031, 1315, 1794
HpyF3I	CTNAG	7 cut(s)	30, 554, 591, 620, 1353, 1545, 1995
HpySE526I	ACGT	5 cut(s)	484, 871, 1031, 1315, 1794
Hsp92II	CATG	9 cut(s)	397, 407, 859, 1349, 1481, 1670, 2055, 2254, 2260
HspAI	GCGC	3 cut(s)	258, 731, 1063
KpnI	GGTACC	1 cut(s)	303
Ksp22I	TGATCA	1 cut(s)	1929
Kzo9I	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
LguI	GCTCTTC	3 cut(s)	669, 1428, 2211
LmnI	GCTCC	3 cut(s)	688, 1223, 2402
LweI	GCATC	7 cut(s)	196, 456, 1009, 1249, 1387, 1925, 2336
MaeII	ACGT	5 cut(s)	484, 871, 1031, 1315, 1794
MaeIII	GTNAC	2 cut(s)	222, 2380
MalI	GATC	6 cut(s)	831, 837, 1135, 1881, 1931, 2304
MbiI	CCGCTC	2 cut(s)	1208, 1733
MboI	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
MfeI	CAATTG	1 cut(s)	2058
MflI	RGATCY	2 cut(s)	829, 1133
MhlI	GDGCHC	8 cut(s)	143, 164, 355, 387, 796, 1506, 1516, 2206
MlyI	GAGTC	2 cut(s)	575, 1232
MmeI	TCCRAC	2 cut(s)	915, 2267
Mph1103I	ATGCAT	1 cut(s)	1402
MroXI	GAANNNNTTC	3 cut(s)	549, 564, 1422
MseI	TTAA	8 cut(s)	170, 633, 684, 1311, 1748, 1854, 2105, 2154
MslI	CAYNNNNRTG	4 cut(s)	392, 2050, 2135, 2255
MspA1I	CMGCKG	4 cut(s)	66, 1202, 1493, 1877
MspI	CCGG	5 cut(s)	76, 735, 1474, 1500, 1521
MspR9I	CCNGG	5 cut(s)	735, 1450, 1501, 2078, 2211
MunI	CAATTG	1 cut(s)	2058
Mva1269I	GAATGC	3 cut(s)	110, 335, 2399
MvaI	CCWGG	3 cut(s)	1450, 2078, 2211
MvnI	CGCG	2 cut(s)	198, 1687
NciI	CCSGG	2 cut(s)	735, 1501
NcoI	CCATGG	1 cut(s)	2051
NdeI	CATATG	1 cut(s)	1576
NdeII	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
NheI	GCTAGC	1 cut(s)	698
NlaIII	CATG	9 cut(s)	397, 407, 859, 1349, 1481, 1670, 2055, 2254, 2260
NlaIV	GGNNCC	5 cut(s)	141, 301, 401, 2241, 2315
NmuCI	GTSAC	2 cut(s)	222, 2380
NotI	GCGGCCGC	1 cut(s)	1203
NruI	TCGCGA	1 cut(s)	198
NsiI	ATGCAT	1 cut(s)	1402
NspI	RCATGY	1 cut(s)	397
PagI	TCATGA	1 cut(s)	2250
PciI	ACATGT	1 cut(s)	393
PciSI	GCTCTTC	3 cut(s)	669, 1428, 2211
PctI	GAATGC	3 cut(s)	110, 335, 2399
PdmI	GAANNNNTTC	3 cut(s)	549, 564, 1422
PfeI	GAWTC	7 cut(s)	1487, 1630, 1670, 1676, 1835, 2082, 2387
PinAI	ACCGGT	1 cut(s)	75
PleI	GAGTC	2 cut(s)	575, 1231
PpsI	GAGTC	2 cut(s)	575, 1231
Ppu21I	YACGTR	2 cut(s)	485, 1032
PscI	ACATGT	1 cut(s)	393
PshAI	GACNNNNGTC	1 cut(s)	1002
PsiI	TTATAA	1 cut(s)	1130
Psp6I	CCWGG	3 cut(s)	1448, 2076, 2209
PspFI	CCCAGC	1 cut(s)	1120
PspGI	CCWGG	3 cut(s)	1448, 2076, 2209
PspN4I	GGNNCC	5 cut(s)	141, 301, 401, 2241, 2315
PspOMI	GGGCCC	1 cut(s)	139
PspPI	GGNCC	5 cut(s)	139, 140, 956, 1452, 1549
PstI	CTGCAG	1 cut(s)	2296
PstNI	CAGNNNCTG	3 cut(s)	1262, 1877, 2390
PsuI	RGATCY	2 cut(s)	829, 1133
PvuII	CAGCTG	2 cut(s)	1493, 1877
RruI	TCGCGA	1 cut(s)	198
RsaI	GTAC	6 cut(s)	52, 301, 870, 1098, 1141, 1985
RsaNI	GTAC	6 cut(s)	51, 300, 869, 1097, 1140, 1984
RseI	CAYNNNNRTG	4 cut(s)	392, 2050, 2135, 2255
SalI	GTCGAC	1 cut(s)	2227
SapI	GCTCTTC	3 cut(s)	669, 1428, 2211
SaqAI	TTAA	8 cut(s)	170, 633, 684, 1311, 1748, 1854, 2105, 2154
Sau3AI	GATC	6 cut(s)	829, 835, 1133, 1879, 1929, 2302
Sau96I	GGNCC	5 cut(s)	139, 140, 956, 1452, 1549
SchI	GAGTC	2 cut(s)	575, 1232
ScrFI	CCNGG	5 cut(s)	735, 1450, 1501, 2078, 2211
SduI	GDGCHC	8 cut(s)	143, 164, 355, 387, 796, 1506, 1516, 2206
SfaNI	GCATC	7 cut(s)	196, 456, 1009, 1249, 1387, 1925, 2336
SfcI	CTRYAG	2 cut(s)	2292, 2338
SinI	GGWCC	1 cut(s)	956
SmiMI	CAYNNNNRTG	4 cut(s)	392, 2050, 2135, 2255
SmlI	CTYRAG	1 cut(s)	703
SmoI	CTYRAG	1 cut(s)	703
SnaBI	TACGTA	1 cut(s)	1032
SspI	AATATT	1 cut(s)	1804
StyD4I	CCNGG	5 cut(s)	733, 1448, 1499, 2076, 2209
StyI	CCWWGG	2 cut(s)	1827, 2051
TaaI	ACNGT	3 cut(s)	451, 868, 1988
TaiI	ACGT	5 cut(s)	487, 874, 1034, 1318, 1797
TaqI	TCGA	4 cut(s)	39, 339, 1457, 2228
TaqII	GACCGA	2 cut(s)	768, 1994
TatI	WGTACW	2 cut(s)	1096, 1983
TauI	GCSGC	4 cut(s)	1205, 1208, 1687, 1772
TfiI	GAWTC	7 cut(s)	1487, 1630, 1670, 1676, 1835, 2082, 2387
Tru1I	TTAA	8 cut(s)	170, 633, 684, 1311, 1748, 1854, 2105, 2154
Tru9I	TTAA	8 cut(s)	170, 633, 684, 1311, 1748, 1854, 2105, 2154
TscAI	CASTG	3 cut(s)	1890, 1949, 1993
TseFI	GTSAC	2 cut(s)	222, 2380
Tsp45I	GTSAC	2 cut(s)	222, 2380
TspDTI	ATGAA	9 cut(s)	678, 1056, 1334, 1551, 1652, 2103, 2267, 2276, 2361
TspGWI	ACGGA	2 cut(s)	500, 1766
TspRI	CASTG	3 cut(s)	1890, 1949, 1993
VneI	GTGCAC	2 cut(s)	792, 1512
VpaK11BI	GGWCC	1 cut(s)	956
XagI	CCTNNNNNAGG	1 cut(s)	774
XapI	RAATTY	5 cut(s)	538, 626, 2090, 2189, 2353
XbaI	TCTAGA	2 cut(s)	1426, 1567
XceI	RCATGY	1 cut(s)	397
XmiI	GTMKAC	1 cut(s)	2228
XmnI	GAANNNNTTC	3 cut(s)	549, 564, 1422
Zsp2I	ATGCAT	1 cut(s)	1402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.