Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

32360279 .. 32364655

Gene Structure 4377 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g40950.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3276 bp

ATGTACCTGCAGCCAAACAGTACCAATGGTGATGACGCCTCTGAAGGCATTAGCGACCCAACTCTTACTGCTGACCTTATCATGCAAACTGTTAGTAGTTGTGTGACTTGTGTCCAATATGAGCAGAGCAAGCAGAGCCGCTGGTACAAGTACATTGCTATTGGGGTTTTCATCTTGCGCACCAGAAACAAGTCACGCCTCCGCAAAGCAAAGGCCCCTCATCATAAATGGTTTCTGAACAAGGTTGCTCGCCTTGATGTGAGACAGAAGGTGTTACACCTTGGATCCTACTTCAAGGATCGTACTGCTAATATCCCTTTATTAGCAGCATCGACAACTCCAACAACGCCACCAGACGCAGCAGCCTCAAATCATGTGATTGACATCCCTCTCCAAGAACGCGTACGGCTTCTGAAAGAGGTTGCTTGCATTGTGAGAGAGAAGAACTTGAATGCACTGCGAGCACGCGGTGGTGTTGATGGTGTTTCAACACTTCTCAGGTCTCACTCTGAGGATCATGCTGATGATGGTGGTCCAGTTCCACAACTAGCATACAAGGGTACCAAACCTGTTTTCTTTCACTTTTTCCTGAAGGCTTGCAATCAGTACACAATCTTTTTCCTCTTCGTCTCAGCTGGTTTGCAATTCGCTATTGAATTCCTGAAGCAAGGTGTCAAACAAGGGTGGCATGACGGTTTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTCCCCTCAATTGGAAACTATCGCAACGAAAAGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTCCTGGTGGATGTTGAAAGAAATGGACAATCTAGAGCGGTTATGATATCTCATGTTTCCGTGGGTGATATAGTTCATTTGAGGAAGGGAGATCATGCTCCTGCTGATGGCTTGTTCATAGATCATGCTCCTGCTGATGGTGTGAAGCTGGTCATGGATGAGGTATTGAGCCCCACAGTTGATTTTGAGAAGAACCCATTTGTGTTGTCTGGTTCAAAAGTTATTGAGGGCCAGGGTCGAATGATTGTGACATCTGTTGGTGTCAATACAGCTTTTGCAGAGATGCTGAGTTTGGTGACTGAGGATCATAATCCAAATAGAAAGACTTTGTTACAAGCTTTGATGGACAAACCATATACTTATATAGAGTATCTTGCGTGTTGTGTTTCTATTCTGATTGCTGTTGTGGTGCTAATACGGTTACTAGTATTCAGGAAGCATGACAACTACAATGACAGGCCAGACATGAAAGGGGAAGTTTCAATCAACCTGCTGATGAAGACATTCGAGAAAATTTACTTGAAAACACAAGGAACGGTCTCCACTTTAGTGAGTGTTCTTGCAACTGTGGTGATAGGGATACAACATGGAATGCCATTTGTGATTTCTCTAGCACTTAGTCAATGGAGGGAGAAGGTGGTCCAAAACCGTGCAGATCCTCAGAATCTATCAGCTTGTGTCACCATGGGCCTCATAACAGTCATCTGCATCGAAACAACCAGTGAGAAAAGGTGTAACCAGATGGAGGTTATGGATTTTTGGATAGGTGGAAAGGATTTAAGCAACGAAGTGGAGTCCGATCAAACCGAACAATTTGCTCTCAAAGGACTACATCAAGAGATTCCTGTTACCCCAGCAAATGAATTGTTCATTTCTTCGATCAACACAAGATGGGGTACTAGTTCTACTGACTTGGAGTCACTGGATCAGACATTCATAACTCTGGAAGAGCGAAAATTGAGTTCTAGTCAGAAATGTAGTGGCATCTTGGTGAGGAAAAACGAGGGCAGTGCACAAGATATGCAGCTGCATATGAATGGAGATGCATCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACTGGGAAAAGTCCTATTATCGAGATTCAGAAGAGAAGCTTTAAGCAGATCATTAAGAAAATGGAGGAGAAGGGTCTTAGACCGATTGCATATGCTTACAAGAAAACAAATGTGGAAGAATTTACAGAACATGGGATGATATTGTTGGCACTGGTGGGAGTCAGATGCCCATATCAAGAAGAGCTTAAGATTGCAGTGGAAGCTTTTCGAGATGCTGGAATAAATATCAAGCTGGTTTCTGAGGATGAGCTCTCGATAGCGACTGCGCAAGCTTCTGATCTTGGAATCTTTAATCAGGGTGATGAGAACATTGCAGGTGAAGCTTTCAGAAGACTGGACTCCATGGAAAGACTAAATAAGGTGAATTTGATATCTGTGATGGGAAGTTCTCTCCCAAAAGACAAGCTTCTGATGGTGGAGCGGTTAAAGAAAAACGGTCACATAGTAGCATTCTATGGAGGGCTAACTGTAAACGATACCCCGACTTTGAAAGAAGCTGATGTCGGAATCACACAAGATATATGGAGCACTGAAATGGCCAGAGACAATGCTGATATTGTAGTTAAGGATGGCTGCTTATGGTTCCTTAACTTGAAGTCTGCGAGATGTGCTTACTACAATATTCAGAACTTCTGTCAAATTCAGCTCACAGCAAGCATATCCGGGTTGCTAGTAACGTTAGTAGCAACAACGCACTCAGGGGACTCTCCATTGACTGCAATTCACTTGATTTGGGTGAACTTGATAATGAACCTTCCAGGTGGCCTAATGATGGTAATGGGGTTAAAAGGCGAGGAGCTACTTGCTCAAGAACCGGCGCCAAGGACTCAACCACTCATAACCAATGCCATCTGGATGCCAAGGACTCAACCACTTATAACCAATGCCATCTGGAGAACCATAGCCATTCAAGTTTCATACCAGGCTACCATCTTGCTGGTCTTGCATTTCATGGGAAATTTAATACCGAGCATGAACCAAGGCGTCCAGGGTACCATGGTTTTCAACATTTACACCTTGTGTCAGGTCCTTAATCTGTTCAGGGTCATGGACATAGTGAACAAGGAAGTGCTAAATGTTGTTCTTCACAGCTATTGGTTTTTGATGGCCGTGGGAGTTGTTATGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGATTAGCCAGTGGTGTGAGGTTGGATGCAGTACAGTGGGCATCCTGCTTCCTTCTTGCTGCTCTTCCATGGGGGTTTGATTGGGCCGTAAACTCGCAACTTGCTTGCTTCCGAAGAGAAAATTGGGCATTGTGGCGTTCACCTATGGGAGCCTCAAGCAGAGGTAGATTCTGCATTTGTGCCTCCTTGTTCCTCATCTTCTCTGTATCATATTGTTTCGAAGATAATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1092

Amino Acids

121.81

Weight (kDa)

7.75

Isoelectric Point (pI)

36.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	13 - 109	2.6e-08	P-type ATPase actuator domain
Cation_ATPase	PF13246	302 - 432	5.8e-12	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	608 - 658	2.9e-07	Cation transporting ATPase, C-terminus
Cation_ATPase_C	PF00689	659 - 787	1.4e-13	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1875, 2762
AarI	CACCTGC	2 cut(s)	2189, 3007
Acc16I	TGCGCA	2 cut(s)	179, 2151
Acc36I	ACCTGC	4 cut(s)	15, 1296, 2189, 3007
Acc65I	GGTACC	2 cut(s)	560, 2876
AccB1I	GGYRCC	3 cut(s)	560, 2701, 2876
AccBSI	CCGCTC	2 cut(s)	836, 2305
AccII	CGCG	2 cut(s)	402, 468
AciI	CCGC	5 cut(s)	139, 202, 468, 836, 2305
AclI	AACGTT	1 cut(s)	2561
AclWI	GGATC	7 cut(s)	279, 292, 306, 522, 1110, 1448, 1731
AcoI	YGGCCR	2 cut(s)	2421, 2991
AcsI	RAATTY	7 cut(s)	656, 797, 1311, 2003, 2248, 2523, 2842
AcuI	CTGAAG	3 cut(s)	63, 611, 683
AcyI	GRCGYC	3 cut(s)	36, 2702, 2868
AdeI	CACNNNGTG	2 cut(s)	470, 2904
AfiI	CCNNNNNNNGG	5 cut(s)	743, 905, 935, 1543, 2656
AflII	CTTAAG	1 cut(s)	2069
AflIII	ACRYGT	2 cut(s)	400, 1860
AhdI	GACNNNNNGTC	1 cut(s)	1714
AhlI	ACTAGT	2 cut(s)	1222, 1697
AjnI	CCWGG	5 cut(s)	801, 1029, 2641, 2805, 2871
AjuI	GAANNNNNNNTTGG	4 cut(s)	557, 589, 3148, 3180
AleI	CACNNNNGTG	1 cut(s)	1346
Alw21I	GWGCWC	4 cut(s)	466, 1813, 2136, 2414
Alw26I	GTCTC	5 cut(s)	256, 507, 634, 1342, 2421
Alw44I	GTGCAC	1 cut(s)	1809
AlwI	GGATC	7 cut(s)	279, 292, 306, 522, 1110, 1448, 1731
AoxI	GGCC	8 cut(s)	213, 1027, 1256, 1486, 2421, 2647, 2991, 3126
ApaLI	GTGCAC	1 cut(s)	1809
ApeKI	GCWGC	8 cut(s)	10, 326, 359, 362, 1822, 1825, 2457, 3101
ApoI	RAATTY	7 cut(s)	656, 797, 1311, 2003, 2248, 2523, 2842
Asp700I	GAANNNNTTC	3 cut(s)	797, 1301, 2088
Asp718I	GGTACC	2 cut(s)	560, 2876
AspLEI	GCGC	3 cut(s)	180, 2152, 2704
AspS9I	GGNCC	7 cut(s)	214, 533, 1027, 1438, 1486, 2911, 3126
AsuC2I	CCSGG	1 cut(s)	2548
AsuII	TTCGAA	1 cut(s)	3261
AvaII	GGWCC	3 cut(s)	533, 1438, 2911
BaeGI	GKGCMC	1 cut(s)	1813
BaeI	ACNNNNGTAYC	4 cut(s)	544, 577, 2868, 2901
BalI	TGGCCA	1 cut(s)	2423
BamHI	GGATCC	1 cut(s)	284
BanI	GGYRCC	3 cut(s)	560, 2701, 2876
BanII	GRGCYC	2 cut(s)	971, 2136
BbsI	GAAGAC	2 cut(s)	1304, 2221
Bbv12I	GWGCWC	4 cut(s)	466, 1813, 2136, 2414
BbvI	GCAGC	8 cut(s)	22, 338, 371, 374, 1812, 1834, 2444, 3088
BceAI	ACGGC	3 cut(s)	422, 2978, 3113
BcgI	CGANNNNNNTGC	5 cut(s)	1595, 1629, 1790, 1824, 3251
BciT130I	CCWGG	5 cut(s)	803, 1031, 2643, 2807, 2873
BciVI	GTATCC	1 cut(s)	1371
BcnI	CCSGG	1 cut(s)	2548
BcoDI	GTCTC	5 cut(s)	256, 507, 634, 1342, 2421
BcuI	ACTAGT	2 cut(s)	1222, 1697
BfaI	CTAG	7 cut(s)	548, 831, 1223, 1409, 1698, 1764, 2555
BfmI	CTRYAG	1 cut(s)	8
BfoI	RGCGCY	1 cut(s)	2705
BfrI	CTTAAG	1 cut(s)	2069
BfuAI	ACCTGC	4 cut(s)	15, 1296, 2189, 3007
BfuI	GTATCC	1 cut(s)	1371
BisI	GCNGC	9 cut(s)	11, 139, 327, 360, 363, 1823, 1826, 2458, 3102
BlsI	GCNGC	9 cut(s)	12, 140, 328, 361, 364, 1824, 1827, 2459, 3103
Bme1390I	CCNGG	6 cut(s)	803, 1031, 2548, 2643, 2807, 2873
Bme18I	GGWCC	3 cut(s)	533, 1438, 2911
BmeRI	GACNNNNNGTC	1 cut(s)	1714
BmgT120I	GGNCC	7 cut(s)	214, 533, 1027, 1438, 1486, 2911, 3126
BmiI	GGNNCC	7 cut(s)	216, 286, 562, 2468, 2703, 2878, 3193
BmrFI	CCNGG	6 cut(s)	803, 1031, 2548, 2643, 2807, 2873
BmrI	ACTGGG	1 cut(s)	1896
BmuI	ACTGGG	1 cut(s)	1896
BoxI	GACNNNNGTC	1 cut(s)	110
BpiI	GAAGAC	2 cut(s)	1304, 2221
BpmI	CTGGAG	1 cut(s)	2797
Bpu14I	TTCGAA	1 cut(s)	3261
BpuEI	CTTGAG	2 cut(s)	2676, 3181
BpuMI	CCSGG	1 cut(s)	2548
BsaBI	GATNNNNATC	2 cut(s)	383, 1107
BsaHI	GRCGYC	3 cut(s)	36, 2702, 2868
BsaI	GGTCTC	2 cut(s)	507, 1342
Bsc4I	CCNNNNNNNGG	5 cut(s)	743, 905, 935, 1543, 2656
Bse118I	RCCGGY	1 cut(s)	2698
Bse1I	ACTGG	7 cut(s)	536, 1518, 1725, 1891, 2040, 2223, 3051
Bse3DI	GCAATG	2 cut(s)	153, 2193
Bse8I	GATNNNNATC	2 cut(s)	383, 1107
BseBI	CCWGG	5 cut(s)	803, 1031, 2643, 2807, 2873
BseGI	GGATG	9 cut(s)	384, 814, 961, 2025, 2134, 2458, 2745, 3073, 3083
BseJI	GATNNNNATC	2 cut(s)	383, 1107
BseLI	CCNNNNNNNGG	5 cut(s)	743, 905, 935, 1543, 2656
BseMI	GCAATG	2 cut(s)	153, 2193
BseMII	CTCAG	8 cut(s)	501, 511, 645, 1076, 1089, 1472, 2115, 2595
BseNI	ACTGG	7 cut(s)	536, 1518, 1725, 1891, 2040, 2223, 3051
BseRI	GAGGAG	2 cut(s)	1964, 2693
BseSI	GKGCMC	1 cut(s)	1813
BseXI	GCAGC	8 cut(s)	22, 338, 371, 374, 1812, 1834, 2444, 3088
BseYI	CCCAGC	1 cut(s)	1651
BsgI	GTGCAG	1 cut(s)	1470
Bsh1236I	CGCG	2 cut(s)	402, 468
BshFI	GGCC	8 cut(s)	215, 1029, 1258, 1488, 2423, 2649, 2993, 3128
BshNI	GGYRCC	3 cut(s)	560, 2701, 2876
BsiHKAI	GWGCWC	4 cut(s)	466, 1813, 2136, 2414
BsiSI	CCGG	2 cut(s)	2547, 2699
BsiWI	CGTACG	1 cut(s)	403
BslFI	GGGAC	1 cut(s)	2600
BslI	CCNNNNNNNGG	5 cut(s)	743, 905, 935, 1543, 2656
BsmAI	GTCTC	5 cut(s)	256, 507, 634, 1342, 2421
BsmBI	CGTCTC	1 cut(s)	634
BsmFI	GGGAC	1 cut(s)	2600
BsmI	GAATGC	3 cut(s)	457, 1395, 2333
BsnI	GGCC	8 cut(s)	215, 1029, 1258, 1488, 2423, 2649, 2993, 3128
Bso31I	GGTCTC	2 cut(s)	507, 1342
Bsp119I	TTCGAA	1 cut(s)	3261
Bsp1286I	GDGCHC	5 cut(s)	466, 971, 1813, 2136, 2414
Bsp19I	CCATGG	4 cut(s)	1482, 2226, 2880, 3110
BspACI	CCGC	5 cut(s)	139, 202, 468, 836, 2305
BspANI	GGCC	8 cut(s)	215, 1029, 1258, 1488, 2423, 2649, 2993, 3128
BspCNI	CTCAG	8 cut(s)	502, 510, 644, 1077, 1090, 1471, 2116, 2594
BspFNI	CGCG	2 cut(s)	402, 468
BspLI	GGNNCC	7 cut(s)	216, 286, 562, 2468, 2703, 2878, 3193
BspMAI	CTGCAG	1 cut(s)	12
BspMI	ACCTGC	4 cut(s)	15, 1296, 2189, 3007
BspPI	GGATC	7 cut(s)	279, 292, 306, 522, 1110, 1448, 1731
BspQI	GCTCTTC	3 cut(s)	1740, 2058, 3111
BspT104I	TTCGAA	1 cut(s)	3261
BspT107I	GGYRCC	3 cut(s)	560, 2701, 2876
BspTI	CTTAAG	1 cut(s)	2069
BspTNI	GGTCTC	2 cut(s)	507, 1342
BsrBI	CCGCTC	2 cut(s)	836, 2305
BsrDI	GCAATG	2 cut(s)	153, 2193
BsrFI	RCCGGY	1 cut(s)	2698
BsrI	ACTGG	7 cut(s)	536, 1518, 1725, 1891, 2040, 2223, 3051
BssAI	RCCGGY	1 cut(s)	2698
BssNI	GRCGYC	3 cut(s)	36, 2702, 2868
BssT1I	CCWWGG	8 cut(s)	280, 1482, 2226, 2705, 2744, 2863, 2880, 3110
Bst2UI	CCWGG	5 cut(s)	803, 1031, 2643, 2807, 2873
Bst6I	CTCTTC	6 cut(s)	629, 1740, 1910, 2058, 3111, 3151
BstACI	GRCGYC	3 cut(s)	36, 2702, 2868
BstAFI	CTTAAG	1 cut(s)	2069
BstBI	TTCGAA	1 cut(s)	3261
BstC8I	GCNNGC	9 cut(s)	131, 250, 427, 462, 466, 598, 2154, 2539, 3148
BstDSI	CCRYGG	6 cut(s)	858, 1482, 2226, 2880, 2994, 3110
BstF5I	GGATG	9 cut(s)	384, 814, 961, 2025, 2134, 2458, 2745, 3073, 3083
BstFNI	CGCG	2 cut(s)	402, 468
BstH2I	RGCGCY	1 cut(s)	2705
BstHHI	GCGC	3 cut(s)	180, 2152, 2704
BstMAI	GTCTC	5 cut(s)	256, 507, 634, 1342, 2421
BstMWI	GCNNNNNNNGC	7 cut(s)	130, 135, 461, 2084, 2204, 2492, 2827
BstNI	CCWGG	5 cut(s)	803, 1031, 2643, 2807, 2873
BstNSI	RCATGY	1 cut(s)	1864
BstPAI	GACNNNNGTC	1 cut(s)	110
BstSCI	CCNGG	6 cut(s)	801, 1029, 2546, 2641, 2805, 2871
BstSFI	CTRYAG	1 cut(s)	8
BstSLI	GKGCMC	1 cut(s)	1813
BstUI	CGCG	2 cut(s)	402, 468
BstV1I	GCAGC	8 cut(s)	22, 338, 371, 374, 1812, 1834, 2444, 3088
BstV2I	GAAGAC	2 cut(s)	1304, 2221
BstX2I	RGATCY	2 cut(s)	284, 1453
BstXI	CCANNNNNNTGG	1 cut(s)	2821
BstYI	RGATCY	2 cut(s)	284, 1453
BsuI	GTATCC	1 cut(s)	1371
BsuRI	GGCC	8 cut(s)	215, 1029, 1258, 1488, 2423, 2649, 2993, 3128
BtgI	CCRYGG	6 cut(s)	858, 1482, 2226, 2880, 2994, 3110
BtsCI	GGATG	9 cut(s)	384, 814, 961, 2025, 2134, 2458, 2745, 3073, 3083
BtsI	GCAGTG	3 cut(s)	455, 1813, 2085
BtsIMutI	CAGTG	9 cut(s)	455, 1525, 1718, 1813, 2033, 2085, 2412, 3058, 3083
BveI	ACCTGC	4 cut(s)	15, 1296, 2189, 3007
Cac8I	GCNNGC	9 cut(s)	131, 250, 427, 462, 466, 598, 2154, 2539, 3148
CfoI	GCGC	3 cut(s)	180, 2152, 2704
Cfr10I	RCCGGY	1 cut(s)	2698
Cfr13I	GGNCC	7 cut(s)	214, 533, 1027, 1438, 1486, 2911, 3126
CseI	GACGC	3 cut(s)	44, 365, 2857
DinI	GGCGCC	1 cut(s)	2703
DraIII	CACNNNGTG	2 cut(s)	470, 2904
DriI	GACNNNNNGTC	1 cut(s)	1714
EaeI	YGGCCR	2 cut(s)	2421, 2991
Eam1104I	CTCTTC	6 cut(s)	629, 1740, 1910, 2058, 3111, 3151
Eam1105I	GACNNNNNGTC	1 cut(s)	1714
EarI	CTCTTC	6 cut(s)	629, 1740, 1910, 2058, 3111, 3151
Ecl136II	GAGCTC	1 cut(s)	2134
Eco130I	CCWWGG	8 cut(s)	280, 1482, 2226, 2705, 2744, 2863, 2880, 3110
Eco24I	GRGCYC	2 cut(s)	971, 2136
Eco31I	GGTCTC	2 cut(s)	507, 1342
Eco32I	GATATC	2 cut(s)	846, 2256
Eco47I	GGWCC	3 cut(s)	533, 1438, 2911
Eco53kI	GAGCTC	1 cut(s)	2134
Eco57I	CTGAAG	3 cut(s)	63, 611, 683
EcoICRI	GAGCTC	1 cut(s)	2134
EcoO109I	RGGNCCY	2 cut(s)	214, 2911
EcoRI	GAATTC	1 cut(s)	656
EcoRII	CCWGG	5 cut(s)	801, 1029, 2641, 2805, 2871
EcoRV	GATATC	2 cut(s)	846, 2256
EcoT14I	CCWWGG	8 cut(s)	280, 1482, 2226, 2705, 2744, 2863, 2880, 3110
EcoT22I	ATGCAT	2 cut(s)	1846, 3274
EcoT38I	GRGCYC	2 cut(s)	971, 2136
EgeI	GGCGCC	1 cut(s)	2703
EheI	GGCGCC	1 cut(s)	2703
ErhI	CCWWGG	8 cut(s)	280, 1482, 2226, 2705, 2744, 2863, 2880, 3110
Esp3I	CGTCTC	1 cut(s)	634
FalI	AAGNNNNNCTT	6 cut(s)	1907, 1939, 2052, 2084, 2274, 2306
FaqI	GGGAC	1 cut(s)	2600
FauNDI	CATATG	2 cut(s)	1830, 1975
Fnu4HI	GCNGC	9 cut(s)	11, 139, 327, 360, 363, 1823, 1826, 2458, 3102
FokI	GGATG	9 cut(s)	371, 821, 968, 2032, 2141, 2465, 2752, 3070, 3080
FriOI	GRGCYC	2 cut(s)	971, 2136
Fsp4HI	GCNGC	9 cut(s)	11, 139, 327, 360, 363, 1823, 1826, 2458, 3102
FspBI	CTAG	7 cut(s)	548, 831, 1223, 1409, 1698, 1764, 2555
FspI	TGCGCA	2 cut(s)	179, 2151
GlaI	GCGC	3 cut(s)	179, 2151, 2703
GluI	GCNGC	9 cut(s)	11, 139, 327, 360, 363, 1823, 1826, 2458, 3102
GsaI	CCCAGC	1 cut(s)	1655
GsuI	CTGGAG	1 cut(s)	2797
HaeII	RGCGCY	1 cut(s)	2705
HaeIII	GGCC	8 cut(s)	215, 1029, 1258, 1488, 2423, 2649, 2993, 3128
HapII	CCGG	2 cut(s)	2547, 2699
HgaI	GACGC	3 cut(s)	44, 365, 2857
HhaI	GCGC	3 cut(s)	180, 2152, 2704
Hin1I	GRCGYC	3 cut(s)	36, 2702, 2868
Hin6I	GCGC	3 cut(s)	178, 2150, 2702
HinP1I	GCGC	3 cut(s)	178, 2150, 2702
HindIII	AAGCTT	6 cut(s)	1134, 1921, 2085, 2154, 2205, 2288
HpaII	CCGG	2 cut(s)	2547, 2699
Hpy166II	GTNNAC	8 cut(s)	609, 1811, 1868, 2356, 2623, 2944, 3133, 3182
Hpy8I	GTNNAC	8 cut(s)	609, 1811, 1868, 2356, 2623, 2944, 3133, 3182
HpyAV	CCTTC	8 cut(s)	38, 262, 586, 877, 1426, 1948, 2648, 3104
HpyCH4IV	ACGT	1 cut(s)	2561
HpyF10VI	GCNNNNNNNGC	7 cut(s)	130, 135, 461, 2084, 2204, 2492, 2827
HpySE526I	ACGT	1 cut(s)	2561
Hsp92I	GRCGYC	3 cut(s)	36, 2702, 2868
HspAI	GCGC	3 cut(s)	178, 2150, 2702
KasI	GGCGCC	1 cut(s)	2701
KpnI	GGTACC	2 cut(s)	564, 2880
LguI	GCTCTTC	3 cut(s)	1740, 2058, 3111
LmnI	GCTCC	6 cut(s)	901, 931, 2302, 2409, 2680, 3191
Lsp1109I	GCAGC	8 cut(s)	22, 338, 371, 374, 1812, 1834, 2444, 3088
MaeI	CTAG	7 cut(s)	548, 831, 1223, 1409, 1698, 1764, 2555
MaeII	ACGT	1 cut(s)	2561
MbiI	CCGCTC	2 cut(s)	836, 2305
MfeI	CAATTG	1 cut(s)	741
MflI	RGATCY	2 cut(s)	284, 1453
MhlI	GDGCHC	5 cut(s)	466, 971, 1813, 2136, 2414
MlsI	TGGCCA	1 cut(s)	2423
MluI	ACGCGT	1 cut(s)	400
MluNI	TGGCCA	1 cut(s)	2423
Mly113I	GGCGCC	1 cut(s)	2702
MlyI	GAGTC	7 cut(s)	1601, 1724, 2052, 2216, 2582, 2704, 2743
MmeI	TCCRAC	3 cut(s)	365, 2368, 3045
Mox20I	TGGCCA	1 cut(s)	2423
Mph1103I	ATGCAT	2 cut(s)	1846, 3274
MroXI	GAANNNNTTC	3 cut(s)	797, 1301, 2088
MscI	TGGCCA	1 cut(s)	2423
MslI	CAYNNNNRTG	8 cut(s)	522, 1346, 1787, 1833, 1875, 2417, 2738, 3017
Msp20I	TGGCCA	1 cut(s)	2423
MspA1I	CMGCKG	3 cut(s)	141, 635, 1825
MspCI	CTTAAG	1 cut(s)	2069
MspI	CCGG	2 cut(s)	2547, 2699
MspR9I	CCNGG	6 cut(s)	803, 1031, 2548, 2643, 2807, 2873
MunI	CAATTG	1 cut(s)	741
Mva1269I	GAATGC	3 cut(s)	457, 1395, 2333
MvaI	CCWGG	5 cut(s)	803, 1031, 2643, 2807, 2873
MvnI	CGCG	2 cut(s)	402, 468
MwoI	GCNNNNNNNGC	7 cut(s)	130, 135, 461, 2084, 2204, 2492, 2827
NarI	GGCGCC	1 cut(s)	2702
NciI	CCSGG	1 cut(s)	2548
NcoI	CCATGG	4 cut(s)	1482, 2226, 2880, 3110
NdeI	CATATG	2 cut(s)	1830, 1975
NlaIV	GGNNCC	7 cut(s)	216, 286, 562, 2468, 2703, 2878, 3193
NmuCI	GTSAC	7 cut(s)	103, 192, 1045, 1093, 1477, 1716, 2321
NsbI	TGCGCA	2 cut(s)	179, 2151
NsiI	ATGCAT	2 cut(s)	1846, 3274
NspI	RCATGY	1 cut(s)	1864
NspV	TTCGAA	1 cut(s)	3261
OliI	CACNNNNGTG	1 cut(s)	1346
PaqCI	CACCTGC	2 cut(s)	2189, 3007
PciI	ACATGT	1 cut(s)	1860
PciSI	GCTCTTC	3 cut(s)	1740, 2058, 3111
PctI	GAATGC	3 cut(s)	457, 1395, 2333
PdmI	GAANNNNTTC	3 cut(s)	797, 1301, 2088
PfeI	GAWTC	6 cut(s)	1462, 1639, 1909, 2169, 2391, 3210
Pfl23II	CGTACG	1 cut(s)	403
PkrI	GCNGC	9 cut(s)	12, 140, 328, 361, 364, 1824, 1827, 2459, 3103
PleI	GAGTC	7 cut(s)	1600, 1723, 2051, 2216, 2582, 2704, 2743
PluTI	GGCGCC	1 cut(s)	2705
PpsI	GAGTC	7 cut(s)	1600, 1723, 2051, 2216, 2582, 2704, 2743
PpuMI	RGGWCCY	1 cut(s)	2911
PscI	ACATGT	1 cut(s)	1860
PshAI	GACNNNNGTC	1 cut(s)	110
PsiI	TTATAA	2 cut(s)	1875, 2762
Psp124BI	GAGCTC	1 cut(s)	2136
Psp1406I	AACGTT	1 cut(s)	2561
Psp5II	RGGWCCY	1 cut(s)	2911
Psp6I	CCWGG	5 cut(s)	801, 1029, 2641, 2805, 2871
PspFI	CCCAGC	1 cut(s)	1651
PspGI	CCWGG	5 cut(s)	801, 1029, 2641, 2805, 2871
PspLI	CGTACG	1 cut(s)	403
PspN4I	GGNNCC	7 cut(s)	216, 286, 562, 2468, 2703, 2878, 3193
PspPI	GGNCC	7 cut(s)	214, 533, 1027, 1438, 1486, 2911, 3126
PspPPI	RGGWCCY	1 cut(s)	2911
PstI	CTGCAG	1 cut(s)	12
PsuI	RGATCY	2 cut(s)	284, 1453
PvuII	CAGCTG	2 cut(s)	635, 1825
RseI	CAYNNNNRTG	8 cut(s)	522, 1346, 1787, 1833, 1875, 2417, 2738, 3017
SacI	GAGCTC	1 cut(s)	2136
SapI	GCTCTTC	3 cut(s)	1740, 2058, 3111
SatI	GCNGC	9 cut(s)	11, 139, 327, 360, 363, 1823, 1826, 2458, 3102
Sau96I	GGNCC	7 cut(s)	214, 533, 1027, 1438, 1486, 2911, 3126
SchI	GAGTC	7 cut(s)	1601, 1724, 2052, 2216, 2582, 2704, 2743
ScrFI	CCNGG	6 cut(s)	803, 1031, 2548, 2643, 2807, 2873
SduI	GDGCHC	5 cut(s)	466, 971, 1813, 2136, 2414
SfcI	CTRYAG	1 cut(s)	8
SfoI	GGCGCC	1 cut(s)	2703
SfuI	TTCGAA	1 cut(s)	3261
SinI	GGWCC	3 cut(s)	533, 1438, 2911
SmiMI	CAYNNNNRTG	8 cut(s)	522, 1346, 1787, 1833, 1875, 2417, 2738, 3017
SmlI	CTYRAG	3 cut(s)	2069, 2691, 3196
SmoI	CTYRAG	3 cut(s)	2069, 2691, 3196
SpeI	ACTAGT	2 cut(s)	1222, 1697
SsiI	CCGC	5 cut(s)	139, 202, 468, 836, 2305
SspDI	GGCGCC	1 cut(s)	2701
SspI	AATATT	1 cut(s)	2506
SspMI	CTAG	7 cut(s)	548, 831, 1223, 1409, 1698, 1764, 2555
SstI	GAGCTC	1 cut(s)	2136
StyD4I	CCNGG	6 cut(s)	801, 1029, 2546, 2641, 2805, 2871
StyI	CCWWGG	8 cut(s)	280, 1482, 2226, 2705, 2744, 2863, 2880, 3110
TaiI	ACGT	1 cut(s)	2564
TaqI	TCGA	9 cut(s)	332, 1036, 1305, 1509, 1676, 1905, 2092, 2138, 3261
TaqII	GACCGA	1 cut(s)	1981
TatI	WGTACW	3 cut(s)	150, 606, 3073
TauI	GCSGC	1 cut(s)	141
TfiI	GAWTC	6 cut(s)	1462, 1639, 1909, 2169, 2391, 3210
TscAI	CASTG	9 cut(s)	462, 1525, 1725, 1813, 2040, 2085, 2419, 3058, 3083
TseFI	GTSAC	7 cut(s)	103, 192, 1045, 1093, 1477, 1716, 2321
TseI	GCWGC	8 cut(s)	10, 326, 359, 362, 1822, 1825, 2457, 3101
Tsp45I	GTSAC	7 cut(s)	103, 192, 1045, 1093, 1477, 1716, 2321
TspGWI	ACGGA	1 cut(s)	847
TspRI	CASTG	9 cut(s)	462, 1525, 1725, 1813, 2040, 2085, 2419, 3058, 3083
Vha464I	CTTAAG	1 cut(s)	2069
VneI	GTGCAC	1 cut(s)	1809
VpaK11BI	GGWCC	3 cut(s)	533, 1438, 2911
XapI	RAATTY	7 cut(s)	656, 797, 1311, 2003, 2248, 2523, 2842
XbaI	TCTAGA	1 cut(s)	830
XceI	RCATGY	1 cut(s)	1864
XmnI	GAANNNNTTC	3 cut(s)	797, 1301, 2088
XspI	CTAG	7 cut(s)	548, 831, 1223, 1409, 1698, 1764, 2555
Zsp2I	ATGCAT	2 cut(s)	1846, 3274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.