Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Reverse (-)

9462004 .. 9465230

Gene Structure 3227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1122800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3150 bp

ATGGCCCTCGAGATAAATACGGAGGATCAAGCTGCTGAAGGTGCCTCTCTTACTGCCGGCTTGATTATGCAGACCATCGGACGAAGGGGAACCTCGTTGAGATATGTTGTTCTAGGCATTTTGTTCTCATCACGCATTAAGAAAACCAGACGTCTGCGTGAAGCTAGTTATGAACCTTTAGCAGCAGCACCAACGCCACCAGCCACTGCCTCTGACCATGTGATTGACATCCCTCTCCAGGAACGGTTTTCAAACAAGGTTGCTCGAATTGTGAAAGAGAAGGACTTGAACTCACTCCGCGGACATGGTGGTGTTGAGGGCATCTTGCCACTTCTCAGGTCTCACTTTGAGGTAGCATGCAACCCTGTAGAAAATAACCGTGTCGTTGATGGTGGTCAAAATCCAGAAGGATGGAACATTACTAAGTCCCCAGTTGAAGCAAAAGGTTTCTTGTACTTTTTGTTTGAGGCGTGCAATCAGTACACAATTTTTTTCCTCTTGCTCTCAGCTGGGTTTTCGTTTGCCATCGAATTCATGAAGCAAGGAGTCAAGGATGGCTGGCATGACGGTGTTGCCATACTCATCACCGCGTTCTTACTAATTGCTTTCCCTTCGGTGCATAACTACCGCAATCAGAGAAAGTCAGCGCAGAAGTACTTGCTGGAGAGGAGCAAACTGATGGTGAATGTCGAAAGAAGTACCACAGAACCTACACCGATTACCATATCTGGCGTTATGGTGGGTGATATAGTTCATTTGAAGGAAGGAGACCGTGTTCTTGTTGACGGTTTGTTCATAGATCACGGTGAGGAACTGGTGGTGGATGAGGTATTGAGCCCGAAAATTGATTGTGAGCAAAATCCATTTGTTTTTTCGGGTTCAAAGGTTGTTAAGGGCCGCGGCCGCATGATTGTGACATCAGTTGGTGCTAATACAGCTATTGCAGAGATGCTGAGCTTGGTGTCTGATCATAATCCAATGGAAAAGACACTGCTACAAGCTTTGATGGACAAACCGAATGCTTGTATGGATTTTCTTGCTTGTTGTGTCTCGATACTGATTGCACTTGTGATGTTGATACGCCTACTATTCTTCAAGAAGTACGAAAACTACAACGAGAGGCCAGACCTGAAAGGTGAAGTTTCAATGAATTTTGTGATGAGGATCTTTGAGAATATCTTCTTGAAACCTCAAGGAAAGGTTTCCATCTTAGCAAGCGTTCTCGCAACTGTGGTAATAGGGATACAACATGGGATGCATGTTGTAATTACAGTTGCCCTTTTTCAATGGAAGGAGAAGGCGGCCGAAAATCAGGCAGATCCACAGGATCTATCAGCTTCTGTCACCATGAGACTCATAACAGTCATCTGCATTGAAACAACGGCAGATCTAATGTGCAACCAGACGGAGGTCAAGGAATTTTGGATCGGCGAAAAGGATTTAAGCAGTGGTGAAGTGGACTCGGAAACTGACCAAGTTCTTGAGGCTCTCCATCAGGGGATCTCAGCTACCGCATCACCAGCAAATGATCGGCTCATTTCTTGGCTGAACACCAGATGGGGTAGTAATGCCGTGTTAGTTGACCGAAGATTTGAAACTGTTGAGCAGAGACAATGGAGCTCTAATGAGAAATATAGAGGGGTCTTAGTGAGGAAAATCGAGAATGATGAGCAGATTATGCAACTTCACTGCGACGGAGAAGCATCAACAATATTAGGCATGTGTTCACATTATTATGATAACAGAGGGGAAAGTCACAATATTGGAAACCAGAAGATAAAATTTGAAGAGGTGATCAAGAAAATGGAGAAGAAGGGTCTTACACCGATTGCATACGCATACAAAAAAATAGAAGCTGAAGATCCTACAGAAGATGGGTTGATTTTGTTGGCACTTGTCGGTCTTAGATGTCCATATCAAGAAAAGCTTAAATTGGCAGTGGAGGCTCTTAGAAAAGCTGGAGTAAGCATCAAACTGGTGTCGGAGGATGAGCTCTCTAAAGTGAGAGCTAGAGCTTCGGAGCTTGGGATCTCCCCTGGCTCAGATGACACAGAGATTGAAGGTGAAGCTTTCCGAAGGCTCAATTCCATGGAAAGGCTGGATAAGGTGGATTTGATCTCTGTGATGGGAAGTTCCCTCCCCAAGGACAAGTTTCTCATGGTGGAGCGGTTAAAGAAAAAAGGTCACATAGTTGCATTCTATGGAGGGCTAACCATCAGTGACACCTTGACCTTAAAAGAAGCTGATATAGGAATTGTTGAGGATCTCTGGAGCACCGAGATGGCCAGAGAGAGCGCCGATCTTATAATTAGAAACGAATGTTTCTTAAGCATGAATATGAAGTCTGGTGCTTGTGCTTACCACAACATTCAGCAATTCTCTCAACTTCAGCTCACCGCTTGCATATCGGGGTTACTAGTAACCCTAGTCGCAACGATGCATTTGGGAGAGTCCCCGCTAACAGCAGTTCACTTGATTTGGGTGAACTTGATAATGTGCCTTCTTGGTGGCCCAATGATGGCGATGGAGTTACAAGGTACCGAACGACACACTCAAAGGCCTGCTAAGAGGACTGAGTCGCTTATAACCAAAGTCATCTGGAGAAAAATAGCGATTCAGGTTTCATATCAGGCTTCCGTCTTGCTGATCTTACATTGCATGGGAAACACAGTACCGAGCATGAACGAAGGCGTCCGGAATACCATGATTTTCAATACTTTCACCTTATGCCAGGTCCTTAATATGTTCAATACCATGGACATATTAAAAAAGGAAGTGCTAGTTGTTGTCCTCCGAAGCCATTGGTTTCTGATAGCGTTGGCGGTTGTTTTGATCATCCAGGTGATCATTGTTGAGTTTGGAAAAGGACTAGCCAGCTGTGTGAGGTTGAATGCATTGCAGTGGCTAATCTGTTGCGCTCTTGCAGCACTTTCGTGGGGTTCTGTTCGGGCCTTCAAATTCCTTTCAGCATACCTCCAAAGAACAACTTCGGCATTGATGGTGAGCTCACACGTTGGATTCTCACATCGGCATCGCAGATTATACATTCCCATTTGGTTTTATGTTCCCACTTGGTTGTTCCTCATCTTCTCTGTATCATTATACTGTATCCGCCCGGAAATTTCTCCACTCGCGCTGTATATCCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1050

Amino Acids

117.35

Weight (kDa)

6.93

Isoelectric Point (pI)

38.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	228 - 321	4.5e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	523 - 640	3.8e-09	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	818 - 987	5.3e-28	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2306, 2585
AatII	GACGTC	1 cut(s)	154
Acc65I	GGTACC	1 cut(s)	2537
AccB1I	GGYRCC	2 cut(s)	41, 2537
AccBSI	CCGCTC	1 cut(s)	2167
AccII	CGCG	4 cut(s)	300, 590, 900, 3134
AccIII	TCCGGA	1 cut(s)	2694
AclWI	GGATC	9 cut(s)	33, 1172, 1313, 1335, 1433, 1508, 1856, 2036, 2271
AcoI	YGGCCR	3 cut(s)	901, 1302, 2283
AcsI	RAATTY	6 cut(s)	530, 1150, 1418, 1781, 2957, 3120
AcuI	CTGAAG	3 cut(s)	57, 1878, 2372
AcyI	GRCGYC	2 cut(s)	151, 2691
AfaI	GTAC	7 cut(s)	455, 482, 656, 700, 1103, 2539, 2673
AfiI	CCNNNNNNNGG	5 cut(s)	238, 1408, 2094, 2143, 2518
AflII	CTTAAG	1 cut(s)	2326
AflIII	ACRYGT	1 cut(s)	3010
AhlI	ACTAGT	1 cut(s)	2416
AjnI	CCWGG	4 cut(s)	237, 2035, 2730, 2838
AleI	CACNNNNGTG	1 cut(s)	2932
AloI	GAACNNNNNNTCC	2 cut(s)	759, 791
Alw21I	GWGCWC	4 cut(s)	1622, 1995, 2276, 3008
Alw26I	GTCTC	5 cut(s)	345, 762, 1054, 1345, 1603
AlwI	GGATC	9 cut(s)	33, 1172, 1313, 1335, 1433, 1508, 1856, 2036, 2271
AlwNI	CAGNNNCTG	2 cut(s)	206, 1340
Ama87I	CYCGRG	1 cut(s)	8
Aor13HI	TCCGGA	1 cut(s)	2694
AoxI	GGCC	9 cut(s)	3, 895, 901, 1121, 1302, 2283, 2509, 2558, 2949
ApeKI	GCWGC	4 cut(s)	32, 182, 185, 2924
ApoI	RAATTY	6 cut(s)	530, 1150, 1418, 1781, 2957, 3120
ArsI	GACNNNNNNTTYG	2 cut(s)	1574, 1606
Asp700I	GAANNNNTTC	3 cut(s)	1178, 1201, 2986
Asp718I	GGTACC	1 cut(s)	2537
AspLEI	GCGC	4 cut(s)	649, 2297, 2918, 3136
AspS9I	GGNCC	5 cut(s)	4, 895, 2510, 2734, 2949
AsuC2I	CCSGG	1 cut(s)	3116
AvaI	CYCGRG	1 cut(s)	8
AvaII	GGWCC	1 cut(s)	2734
BalI	TGGCCA	1 cut(s)	2285
BanI	GGYRCC	2 cut(s)	41, 2537
BanII	GRGCYC	4 cut(s)	839, 1622, 1995, 3008
Bbv12I	GWGCWC	4 cut(s)	1622, 1995, 2276, 3008
BbvI	GCAGC	4 cut(s)	19, 194, 197, 2936
BceAI	ACGGC	2 cut(s)	1398, 1556
BcgI	CGANNNNNNTGC	2 cut(s)	2913, 2947
BciT130I	CCWGG	4 cut(s)	239, 2037, 2732, 2840
BciVI	GTATCC	2 cut(s)	1236, 3119
BclI	TGATCA	4 cut(s)	967, 1794, 2832, 2844
BcnI	CCSGG	1 cut(s)	3116
BcoDI	GTCTC	5 cut(s)	345, 762, 1054, 1345, 1603
BcuI	ACTAGT	1 cut(s)	2416
BfaI	CTAG	7 cut(s)	113, 165, 2010, 2417, 2426, 2780, 2870
BfmI	CTRYAG	2 cut(s)	366, 1866
BfoI	RGCGCY	1 cut(s)	2298
BfrI	CTTAAG	1 cut(s)	2326
BfuI	GTATCC	2 cut(s)	1236, 3119
BglII	AGATCT	1 cut(s)	1387
BisI	GCNGC	8 cut(s)	33, 183, 186, 898, 901, 904, 1302, 2925
BlpI	GCTNAGC	1 cut(s)	953
BlsI	GCNGC	8 cut(s)	34, 184, 187, 899, 902, 905, 1303, 2926
BmcAI	AGTACT	1 cut(s)	656
Bme1390I	CCNGG	5 cut(s)	239, 2037, 2732, 2840, 3116
Bme18I	GGWCC	1 cut(s)	2734
BmeT110I	CYCGRG	1 cut(s)	8
BmgT120I	GGNCC	5 cut(s)	4, 895, 2510, 2734, 2949
BmiI	GGNNCC	3 cut(s)	43, 91, 2539
BmrFI	CCNGG	5 cut(s)	239, 2037, 2732, 2840, 3116
BmrI	ACTGGG	1 cut(s)	425
BmsI	GCATC	8 cut(s)	330, 939, 1245, 1523, 1712, 1977, 2427, 3040
BmuI	ACTGGG	1 cut(s)	425
BoxI	GACNNNNGTC	1 cut(s)	1409
BpmI	CTGGAG	5 cut(s)	221, 683, 1980, 2290, 2620
Bpu1102I	GCTNAGC	1 cut(s)	953
BpuEI	CTTGAG	2 cut(s)	1176, 1502
BpuMI	CCSGG	1 cut(s)	3116
BsaBI	GATNNNNATC	3 cut(s)	227, 972, 1163
BsaHI	GRCGYC	2 cut(s)	151, 2691
BsaI	GGTCTC	2 cut(s)	345, 762
BsaJI	CCNNGG	6 cut(s)	298, 898, 2035, 2088, 2142, 2754
BsaWI	WCCGGW	1 cut(s)	2694
BsaXI	ACNNNNNCTCC	2 cut(s)	759, 789
Bsc4I	CCNNNNNNNGG	5 cut(s)	238, 1408, 2094, 2143, 2518
Bse118I	RCCGGY	1 cut(s)	56
Bse1I	ACTGG	3 cut(s)	431, 819, 1980
Bse3DI	GCAATG	2 cut(s)	2653, 2894
Bse8I	GATNNNNATC	3 cut(s)	227, 972, 1163
BseAI	TCCGGA	1 cut(s)	2694
BseBI	CCWGG	4 cut(s)	239, 2037, 2732, 2840
BseDI	CCNNGG	6 cut(s)	298, 898, 2035, 2088, 2142, 2754
BseGI	GGATG	7 cut(s)	228, 416, 559, 829, 1260, 1993, 2835
BseJI	GATNNNNATC	3 cut(s)	227, 972, 1163
BseLI	CCNNNNNNNGG	5 cut(s)	238, 1408, 2094, 2143, 2518
BseMI	GCAATG	2 cut(s)	2653, 2894
BseMII	CTCAG	6 cut(s)	349, 519, 944, 1518, 2055, 2565
BseNI	ACTGG	3 cut(s)	431, 819, 1980
BseRI	GAGGAG	1 cut(s)	682
BseX3I	CGGCCG	2 cut(s)	901, 1302
BseXI	GCAGC	4 cut(s)	19, 194, 197, 2936
BseYI	CCCAGC	1 cut(s)	509
Bsh1236I	CGCG	4 cut(s)	300, 590, 900, 3134
Bsh1285I	CGRYCG	2 cut(s)	904, 1305
BshFI	GGCC	9 cut(s)	5, 897, 903, 1123, 1304, 2285, 2511, 2560, 2951
BshNI	GGYRCC	2 cut(s)	41, 2537
BsiEI	CGRYCG	2 cut(s)	904, 1305
BsiHKAI	GWGCWC	4 cut(s)	1622, 1995, 2276, 3008
BsiHKCI	CYCGRG	1 cut(s)	8
BsiSI	CCGG	3 cut(s)	57, 2695, 3116
BslFI	GGGAC	2 cut(s)	412, 2437
BslI	CCNNNNNNNGG	5 cut(s)	238, 1408, 2094, 2143, 2518
BsmAI	GTCTC	5 cut(s)	345, 762, 1054, 1345, 1603
BsmFI	GGGAC	2 cut(s)	412, 2437
BsmI	GAATGC	3 cut(s)	1024, 2195, 2896
BsnI	GGCC	9 cut(s)	5, 897, 903, 1123, 1304, 2285, 2511, 2560, 2951
Bso31I	GGTCTC	2 cut(s)	345, 762
BsoBI	CYCGRG	1 cut(s)	8
Bsp1286I	GDGCHC	5 cut(s)	839, 1622, 1995, 2276, 3008
Bsp13I	TCCGGA	1 cut(s)	2694
Bsp1720I	GCTNAGC	1 cut(s)	953
Bsp19I	CCATGG	2 cut(s)	2088, 2754
BspANI	GGCC	9 cut(s)	5, 897, 903, 1123, 1304, 2285, 2511, 2560, 2951
BspCNI	CTCAG	6 cut(s)	348, 518, 945, 1517, 2054, 2566
BspEI	TCCGGA	1 cut(s)	2694
BspFNI	CGCG	4 cut(s)	300, 590, 900, 3134
BspHI	TCATGA	1 cut(s)	534
BspLI	GGNNCC	3 cut(s)	43, 91, 2539
BspPI	GGATC	9 cut(s)	33, 1172, 1313, 1335, 1433, 1508, 1856, 2036, 2271
BspT107I	GGYRCC	2 cut(s)	41, 2537
BspTI	CTTAAG	1 cut(s)	2326
BspTNI	GGTCTC	2 cut(s)	345, 762
BsrBI	CCGCTC	1 cut(s)	2167
BsrDI	GCAATG	2 cut(s)	2653, 2894
BsrFI	RCCGGY	1 cut(s)	56
BsrI	ACTGG	3 cut(s)	431, 819, 1980
BssAI	RCCGGY	1 cut(s)	56
BssECI	CCNNGG	6 cut(s)	298, 898, 2035, 2088, 2142, 2754
BssNI	GRCGYC	2 cut(s)	151, 2691
BssT1I	CCWWGG	3 cut(s)	2088, 2142, 2754
Bst2UI	CCWGG	4 cut(s)	239, 2037, 2732, 2840
Bst6I	CTCTTC	1 cut(s)	1782
BstACI	GRCGYC	2 cut(s)	151, 2691
BstAFI	CTTAAG	1 cut(s)	2326
BstAPI	GCANNNNNTGC	1 cut(s)	1678
BstC8I	GCNNGC	8 cut(s)	58, 358, 472, 560, 1216, 2401, 2562, 2875
BstDSI	CCRYGG	4 cut(s)	298, 898, 2088, 2754
BstENI	CCTNNNNNAGG	1 cut(s)	2141
BstF5I	GGATG	7 cut(s)	228, 416, 559, 829, 1260, 1993, 2835
BstFNI	CGCG	4 cut(s)	300, 590, 900, 3134
BstH2I	RGCGCY	1 cut(s)	2298
BstHHI	GCGC	4 cut(s)	649, 2297, 2918, 3136
BstMAI	GTCTC	5 cut(s)	345, 762, 1054, 1345, 1603
BstMCI	CGRYCG	2 cut(s)	904, 1305
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 903, 935, 1520, 1678, 1943, 2924
BstNI	CCWGG	4 cut(s)	239, 2037, 2732, 2840
BstNSI	RCATGY	3 cut(s)	360, 1262, 1723
BstPAI	GACNNNNGTC	1 cut(s)	1409
BstSCI	CCNGG	5 cut(s)	237, 2035, 2730, 2838, 3114
BstSFI	CTRYAG	2 cut(s)	366, 1866
BstUI	CGCG	4 cut(s)	300, 590, 900, 3134
BstV1I	GCAGC	4 cut(s)	19, 194, 197, 2936
BstX2I	RGATCY	8 cut(s)	1164, 1318, 1327, 1387, 1500, 1861, 2028, 2263
BstXI	CCANNNNNNTGG	1 cut(s)	411
BstYI	RGATCY	8 cut(s)	1164, 1318, 1327, 1387, 1500, 1861, 2028, 2263
BstZI	CGGCCG	2 cut(s)	901, 1302
BsuI	GTATCC	2 cut(s)	1236, 3119
BsuRI	GGCC	9 cut(s)	5, 897, 903, 1123, 1304, 2285, 2511, 2560, 2951
BtgI	CCRYGG	4 cut(s)	298, 898, 2088, 2754
BtgZI	GCGATG	2 cut(s)	2537, 3017
BtsCI	GGATG	7 cut(s)	228, 416, 559, 829, 1260, 1993, 2835
BtsI	GCAGTG	6 cut(s)	204, 989, 1453, 1687, 1944, 2906
BtsIMutI	CAGTG	7 cut(s)	204, 989, 1453, 1687, 1944, 2224, 2906
Cac8I	GCNNGC	8 cut(s)	58, 358, 472, 560, 1216, 2401, 2562, 2875
CaiI	CAGNNNCTG	2 cut(s)	206, 1340
CciI	TCATGA	1 cut(s)	534
CciNI	GCGGCCGC	1 cut(s)	901
CfoI	GCGC	4 cut(s)	649, 2297, 2918, 3136
Cfr10I	RCCGGY	1 cut(s)	56
Cfr13I	GGNCC	5 cut(s)	4, 895, 2510, 2734, 2949
Cfr42I	CCGCGG	2 cut(s)	301, 901
CseI	GACGC	1 cut(s)	2680
Csp6I	GTAC	7 cut(s)	454, 481, 655, 699, 1102, 2538, 2672
CviQI	GTAC	7 cut(s)	454, 481, 655, 699, 1102, 2538, 2672
EaeI	YGGCCR	3 cut(s)	901, 1302, 2283
EagI	CGGCCG	2 cut(s)	901, 1302
Eam1104I	CTCTTC	1 cut(s)	1782
EarI	CTCTTC	1 cut(s)	1782
EciI	GGCGGA	1 cut(s)	3101
Ecl136II	GAGCTC	3 cut(s)	1620, 1993, 3006
EclXI	CGGCCG	2 cut(s)	901, 1302
Eco130I	CCWWGG	3 cut(s)	2088, 2142, 2754
Eco147I	AGGCCT	1 cut(s)	2560
Eco24I	GRGCYC	4 cut(s)	839, 1622, 1995, 3008
Eco31I	GGTCTC	2 cut(s)	345, 762
Eco47I	GGWCC	1 cut(s)	2734
Eco52I	CGGCCG	2 cut(s)	901, 1302
Eco53kI	GAGCTC	3 cut(s)	1620, 1993, 3006
Eco57I	CTGAAG	3 cut(s)	57, 1878, 2372
Eco88I	CYCGRG	1 cut(s)	8
EcoICRI	GAGCTC	3 cut(s)	1620, 1993, 3006
EcoNI	CCTNNNNNAGG	1 cut(s)	2141
EcoO109I	RGGNCCY	1 cut(s)	2734
EcoRI	GAATTC	1 cut(s)	530
EcoRII	CCWGG	4 cut(s)	237, 2035, 2730, 2838
EcoT14I	CCWWGG	3 cut(s)	2088, 2142, 2754
EcoT22I	ATGCAT	3 cut(s)	1260, 2442, 2896
EcoT38I	GRGCYC	4 cut(s)	839, 1622, 1995, 3008
ErhI	CCWWGG	3 cut(s)	2088, 2142, 2754
FalI	AAGNNNNNCTT	4 cut(s)	1911, 1943, 2971, 3003
FaqI	GGGAC	2 cut(s)	412, 2437
FauI	CCCGC	1 cut(s)	2463
FbaI	TGATCA	4 cut(s)	967, 1794, 2832, 2844
Fnu4HI	GCNGC	8 cut(s)	33, 183, 186, 898, 901, 904, 1302, 2925
FokI	GGATG	7 cut(s)	215, 423, 566, 836, 1267, 2000, 2822
FriOI	GRGCYC	4 cut(s)	839, 1622, 1995, 3008
Fsp4HI	GCNGC	8 cut(s)	33, 183, 186, 898, 901, 904, 1302, 2925
FspBI	CTAG	7 cut(s)	113, 165, 2010, 2417, 2426, 2780, 2870
GlaI	GCGC	4 cut(s)	648, 2296, 2917, 3135
GluI	GCNGC	8 cut(s)	33, 183, 186, 898, 901, 904, 1302, 2925
GsaI	CCCAGC	1 cut(s)	513
GsuI	CTGGAG	5 cut(s)	221, 683, 1980, 2290, 2620
HaeII	RGCGCY	1 cut(s)	2298
HaeIII	GGCC	9 cut(s)	5, 897, 903, 1123, 1304, 2285, 2511, 2560, 2951
HapII	CCGG	3 cut(s)	57, 2695, 3116
HgaI	GACGC	1 cut(s)	2680
HhaI	GCGC	4 cut(s)	649, 2297, 2918, 3136
Hin1I	GRCGYC	2 cut(s)	151, 2691
Hin6I	GCGC	4 cut(s)	647, 2295, 2916, 3134
HinP1I	GCGC	4 cut(s)	647, 2295, 2916, 3134
HincII	GTYRAC	2 cut(s)	784, 1582
HindII	GTYRAC	2 cut(s)	784, 1582
HindIII	AAGCTT	3 cut(s)	999, 1925, 2067
HinfI	GANTC	7 cut(s)	546, 1353, 1460, 2450, 2576, 2614, 3018
HpaII	CCGG	3 cut(s)	57, 2695, 3116
Hpy166II	GTNNAC	7 cut(s)	483, 784, 1459, 1582, 1727, 2470, 2485
Hpy8I	GTNNAC	7 cut(s)	483, 784, 1459, 1582, 1727, 2470, 2485
Hpy99I	CGWCG	1 cut(s)	1697
HpyCH4IV	ACGT	2 cut(s)	151, 3012
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 903, 935, 1520, 1678, 1943, 2924
HpySE526I	ACGT	2 cut(s)	151, 3012
Hsp92I	GRCGYC	2 cut(s)	151, 2691
HspAI	GCGC	4 cut(s)	647, 2295, 2916, 3134
Kpn2I	TCCGGA	1 cut(s)	2694
KpnI	GGTACC	1 cut(s)	2541
KroI	GCCGGC	1 cut(s)	56
KroNI	GCCGGC	1 cut(s)	58
Ksp22I	TGATCA	4 cut(s)	967, 1794, 2832, 2844
KspI	CCGCGG	2 cut(s)	301, 901
LmnI	GCTCC	5 cut(s)	669, 1617, 2020, 2164, 2271
Lsp1109I	GCAGC	4 cut(s)	19, 194, 197, 2936
LweI	GCATC	8 cut(s)	330, 939, 1245, 1523, 1712, 1977, 2427, 3040
MaeI	CTAG	7 cut(s)	113, 165, 2010, 2417, 2426, 2780, 2870
MaeII	ACGT	2 cut(s)	151, 3012
MaeIII	GTNAC	8 cut(s)	913, 1342, 1754, 2183, 2219, 2412, 2419, 2529
MbiI	CCGCTC	1 cut(s)	2167
MboII	GAAGA	9 cut(s)	1084, 1171, 1599, 1786, 1799, 1822, 1871, 1883, 3079
MflI	RGATCY	8 cut(s)	1164, 1318, 1327, 1387, 1500, 1861, 2028, 2263
MhlI	GDGCHC	5 cut(s)	839, 1622, 1995, 2276, 3008
MlsI	TGGCCA	1 cut(s)	2285
MluNI	TGGCCA	1 cut(s)	2285
MlyI	GAGTC	5 cut(s)	555, 1347, 1454, 2459, 2585
MmeI	TCCRAC	2 cut(s)	1962, 2995
Mox20I	TGGCCA	1 cut(s)	2285
Mph1103I	ATGCAT	3 cut(s)	1260, 2442, 2896
MroI	TCCGGA	1 cut(s)	2694
MroNI	GCCGGC	1 cut(s)	56
MroXI	GAANNNNTTC	3 cut(s)	1178, 1201, 2986
MscI	TGGCCA	1 cut(s)	2285
MslI	CAYNNNNRTG	9 cut(s)	309, 567, 911, 1734, 2279, 2336, 2840, 2899, 2932
Msp20I	TGGCCA	1 cut(s)	2285
MspA1I	CMGCKG	4 cut(s)	300, 509, 900, 2877
MspCI	CTTAAG	1 cut(s)	2326
MspI	CCGG	3 cut(s)	57, 2695, 3116
MspR9I	CCNGG	5 cut(s)	239, 2037, 2732, 2840, 3116
Mva1269I	GAATGC	3 cut(s)	1024, 2195, 2896
MvaI	CCWGG	4 cut(s)	239, 2037, 2732, 2840
MvnI	CGCG	4 cut(s)	300, 590, 900, 3134
MwoI	GCNNNNNNNGC	7 cut(s)	41, 903, 935, 1520, 1678, 1943, 2924
NaeI	GCCGGC	1 cut(s)	58
NciI	CCSGG	1 cut(s)	3116
NcoI	CCATGG	2 cut(s)	2088, 2754
NgoMIV	GCCGGC	1 cut(s)	56
NlaIV	GGNNCC	3 cut(s)	43, 91, 2539
NmuCI	GTSAC	5 cut(s)	913, 1342, 1754, 2183, 2219
NotI	GCGGCCGC	1 cut(s)	901
NsiI	ATGCAT	3 cut(s)	1260, 2442, 2896
NspI	RCATGY	3 cut(s)	360, 1262, 1723
OliI	CACNNNNGTG	1 cut(s)	2932
PaeI	GCATGC	1 cut(s)	360
PaeR7I	CTCGAG	1 cut(s)	8
PagI	TCATGA	1 cut(s)	534
PceI	AGGCCT	1 cut(s)	2560
PctI	GAATGC	3 cut(s)	1024, 2195, 2896
PdiI	GCCGGC	1 cut(s)	58
PdmI	GAANNNNTTC	3 cut(s)	1178, 1201, 2986
PfeI	GAWTC	2 cut(s)	2614, 3018
PflFI	GACNNNGTC	1 cut(s)	2575
PfoI	TCCNGGA	1 cut(s)	237
PkrI	GCNGC	8 cut(s)	34, 184, 187, 899, 902, 905, 1303, 2926
PleI	GAGTC	5 cut(s)	554, 1347, 1454, 2458, 2584
PpsI	GAGTC	5 cut(s)	554, 1347, 1454, 2458, 2584
PpuMI	RGGWCCY	1 cut(s)	2734
PshAI	GACNNNNGTC	1 cut(s)	1409
PsiI	TTATAA	2 cut(s)	2306, 2585
Psp124BI	GAGCTC	3 cut(s)	1622, 1995, 3008
Psp5II	RGGWCCY	1 cut(s)	2734
Psp6I	CCWGG	4 cut(s)	237, 2035, 2730, 2838
PspFI	CCCAGC	1 cut(s)	509
PspGI	CCWGG	4 cut(s)	237, 2035, 2730, 2838
PspN4I	GGNNCC	3 cut(s)	43, 91, 2539
PspPI	GGNCC	5 cut(s)	4, 895, 2510, 2734, 2949
PspPPI	RGGWCCY	1 cut(s)	2734
PstNI	CAGNNNCTG	2 cut(s)	206, 1340
PsuI	RGATCY	8 cut(s)	1164, 1318, 1327, 1387, 1500, 1861, 2028, 2263
PsyI	GACNNNGTC	1 cut(s)	2575
PvuII	CAGCTG	2 cut(s)	509, 2877
RsaI	GTAC	7 cut(s)	455, 482, 656, 700, 1103, 2539, 2673
RsaNI	GTAC	7 cut(s)	454, 481, 655, 699, 1102, 2538, 2672
RseI	CAYNNNNRTG	9 cut(s)	309, 567, 911, 1734, 2279, 2336, 2840, 2899, 2932
SacI	GAGCTC	3 cut(s)	1622, 1995, 3008
SacII	CCGCGG	2 cut(s)	301, 901
SatI	GCNGC	8 cut(s)	33, 183, 186, 898, 901, 904, 1302, 2925
Sau96I	GGNCC	5 cut(s)	4, 895, 2510, 2734, 2949
ScaI	AGTACT	1 cut(s)	656
SchI	GAGTC	5 cut(s)	555, 1347, 1454, 2459, 2585
ScrFI	CCNGG	5 cut(s)	239, 2037, 2732, 2840, 3116
SduI	GDGCHC	5 cut(s)	839, 1622, 1995, 2276, 3008
SfaNI	GCATC	8 cut(s)	330, 939, 1245, 1523, 1712, 1977, 2427, 3040
SfcI	CTRYAG	2 cut(s)	366, 1866
Sfr274I	CTCGAG	1 cut(s)	8
Sfr303I	CCGCGG	2 cut(s)	301, 901
SgrBI	CCGCGG	2 cut(s)	301, 901
SinI	GGWCC	1 cut(s)	2734
SlaI	CTCGAG	1 cut(s)	8
SmiMI	CAYNNNNRTG	9 cut(s)	309, 567, 911, 1734, 2279, 2336, 2840, 2899, 2932
SmlI	CTYRAG	4 cut(s)	8, 1191, 1481, 2326
SmoI	CTYRAG	4 cut(s)	8, 1191, 1481, 2326
SpeI	ACTAGT	1 cut(s)	2416
SphI	GCATGC	1 cut(s)	360
SseBI	AGGCCT	1 cut(s)	2560
SspI	AATATT	2 cut(s)	1713, 1762
SspMI	CTAG	7 cut(s)	113, 165, 2010, 2417, 2426, 2780, 2870
SstI	GAGCTC	3 cut(s)	1622, 1995, 3008
StuI	AGGCCT	1 cut(s)	2560
StyD4I	CCNGG	5 cut(s)	237, 2035, 2730, 2838, 3114
StyI	CCWWGG	3 cut(s)	2088, 2142, 2754
TaiI	ACGT	2 cut(s)	154, 3015
TaqI	TCGA	6 cut(s)	9, 265, 528, 690, 1052, 1659
TaqII	GACCGA	2 cut(s)	1599, 1889
TatI	WGTACW	3 cut(s)	453, 480, 654
TauI	GCSGC	4 cut(s)	900, 903, 906, 1304
TfiI	GAWTC	2 cut(s)	2614, 3018
TscAI	CASTG	7 cut(s)	211, 996, 1453, 1694, 1944, 2224, 2906
TseFI	GTSAC	5 cut(s)	913, 1342, 1754, 2183, 2219
TseI	GCWGC	4 cut(s)	32, 182, 185, 2924
Tsp45I	GTSAC	5 cut(s)	913, 1342, 1754, 2183, 2219
TspGWI	ACGGA	5 cut(s)	35, 1421, 1710, 2626, 3134
TspRI	CASTG	7 cut(s)	211, 996, 1453, 1694, 1944, 2224, 2906
Tth111I	GACNNNGTC	1 cut(s)	2575
Vha464I	CTTAAG	1 cut(s)	2326
VpaK11BI	GGWCC	1 cut(s)	2734
XagI	CCTNNNNNAGG	1 cut(s)	2141
XapI	RAATTY	6 cut(s)	530, 1150, 1418, 1781, 2957, 3120
XceI	RCATGY	3 cut(s)	360, 1262, 1723
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2095
XhoI	CTCGAG	1 cut(s)	8
XmnI	GAANNNNTTC	3 cut(s)	1178, 1201, 2986
XspI	CTAG	7 cut(s)	113, 165, 2010, 2417, 2426, 2780, 2870
ZraI	GACGTC	1 cut(s)	152
ZrmI	AGTACT	1 cut(s)	656
Zsp2I	ATGCAT	3 cut(s)	1260, 2442, 2896

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.