Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

77100406 .. 77104440

Gene Structure 4035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG541500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGAAGCAAGGAGTCAAACAAGGGTGGCATGACGGTGTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTTCCTTCAGTTGGGAACTATCGCAACGAGAGGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTTCTGGTGGATGTTGAAAGAAATGGAGAATCTAGAGCGGTTATGATATCTCATGTTTCGGTGGGTGATATAGTTCATTTGAAGAAGGGAGACCATGCTCCTGCTGATGGCTTGTTCATAGATCATGGTGTGGAACTGGTCTTGGATGAGGTATTGAGCCCCACAATTGATTTTGAAAACAACCCATTTGTGTTGTCTGGTTCAAAAGTTATTGAGGGCCAGGGTCGAATGATTGTGACATCTGTTGGTGTCGATACAGCTTTTGCAGATATGCTGAGCTTGGTGACTGAGGATCCTAATCCAAATAGGAAGACTCTGTTACAAGCTTTGATGGACAAACCATATACTTATATGGAGTATCTCGCATGTTGTGTTTCAATTCTGATTGCTTTTGTGGTGCTAATACGGTTTCTAGTATTCAGGAAGCATGACAACTATAATGACAGGCCAGACCTGAAGGGGGAAGTTTCAATGAACTTGCTGATGAAGATTTTCGAGAAAATTTACTTGAAAACTCAAGGAAGGGTCTCCACTTTAGCAAGTGTTCTTGCTACTGTGGTAATTGGGATACAACATGGAATGCCCTTTGTGATTTCCCTTGCACTTTGTCAATGGAAGGAGAAGGTGGTCCAAAATGGGGCAGATCCTCGGAATCTTTCAGCTTGTGTCACCATGGGACTCATAACAGTCATCTGCATTGAAACAACTAGTGAGAAAATGTGTAACCAGATAGAGGTTATGGACTTTTGGATGGGTGATAAGGATTTAAGCAATGAAGTGGAGTCTGATCAAACTGAACAATTTGCTCTCAAAGGACTACGTCAAGAGATTTCTGCTACCCCAGCAAATGATTCGTTCATTTCTTCGATCAAGACCAGATGGGGCGTTACTGACATGGAGGTATTGGATCAGACATTTATAACTCTGGAAGAGAGAAAATTGAGTTCTGATGAGAAATGTAGTGGCATCCTAATGAGGAAAACAGAGAGCAGTGCACAAGATATGCAGCTGCATATGAGTGGAGATGCATCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACAGGGGAAAGTCGTATTCTGGAAATTCAGAAGAGAAAGTTTAAGCAGGTCATTAAGAACATGGAGGAGAAGGGTCTTAGACCGATTGCATATGCTTACAAGAAAACAAATGTTCAAGAATTTACAGAAAATGGGTTGAAATTGTTGGCACTGGTGGGGGTCAGATGCCCATATCAAGAAGAGCTTAAAATTGCAGTGGAAGCTTTTAGAAAGGCCGGAATAAGAATCAAGCTGGTATCCGAGGATGAGCTCTCAATAGTGACTGAGCGAGCTTCTGATCTTGGAATCTTTAACCCTGGCAGCCTAATGATGGTAATGGAGTTAGAACGCCAGGAGCCACTTGCACAAGAACCAGCGCCAAGGACTCAAGAACCGGCCCCAAGTACTCAAGAACCGGCACGGACTCAGCCACTTATAACCAAAGCCATTTGGGGAAACATAGCCAGTCAAGTTTCATACCAGGCTTCCATCTTGCTGGTCTTGCATTTCATGGGAAATGCAATAGCGAGCATGAACCAAGGCGTCCGGGGTACCATGGTTTTCAATATTTACACCTTGTGTCAGGTCCTTAATCTGTTCAGGGCTATGGACCTAGTGAACAAGGAAGTGCTAAATGTTGTTCTTCACAGCTATTGGTTTTTGATGGCCTTGGGGGCTGTTATGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGACTAGCCAGTGGTGTGAGGTTGGATGCAATACATTGGGCATCCTGCTTCCTTCTTGCTGCTCTTTCATGGCCGTTTGATTGGGTCATAACTTCGCAGTTTCGAAGAGAAAATTTGGCATGGAGGAGAGCACCTATGGGGATGTCAAGATTTTACATTTGTGCTTCGTTGTTCCTCATTTTCTCTGTATCATATTGTTTCGAATCAGATTATGCACACACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

709

Amino Acids

79.73

Weight (kDa)

5.99

Isoelectric Point (pI)

34.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	49 - 141	4.1e-09	P-type ATPase actuator domain
Cation_ATPase	PF13246	315 - 461	4.8e-11	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	532 - 663	3.3e-15	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1058, 1197, 1622
AarI	CACCTGC	1 cut(s)	1867
Acc36I	ACCTGC	2 cut(s)	1243, 1867
Acc65I	GGTACC	1 cut(s)	1736
AccB1I	GGYRCC	1 cut(s)	1736
AccBSI	CCGCTC	1 cut(s)	176
AciI	CCGC	1 cut(s)	176
AclWI	GGATC	4 cut(s)	425, 438, 776, 1053
AcoI	YGGCCR	1 cut(s)	1973
AcsI	RAATTY	5 cut(s)	137, 639, 1230, 1325, 2014
AcuI	CTGAAG	2 cut(s)	63, 614
AcyI	GRCGYC	1 cut(s)	1728
AdeI	CACNNNGTG	1 cut(s)	1764
AfaI	GTAC	2 cut(s)	1591, 1738
AfiI	CCNNNNNNNGG	6 cut(s)	83, 245, 598, 775, 871, 1516
AflIII	ACRYGT	1 cut(s)	1182
AhlI	ACTAGT	1 cut(s)	845
AjnI	CCWGG	4 cut(s)	357, 1501, 1536, 1665
Alw21I	GWGCWC	3 cut(s)	1135, 1458, 2035
Alw26I	GTCTC	2 cut(s)	222, 670
Alw44I	GTGCAC	1 cut(s)	1131
AlwI	GGATC	4 cut(s)	425, 438, 776, 1053
AoxI	GGCC	6 cut(s)	355, 584, 1419, 1581, 1851, 1973
ApaLI	GTGCAC	1 cut(s)	1131
ApeKI	GCWGC	4 cut(s)	1144, 1147, 1506, 1961
ApoI	RAATTY	5 cut(s)	137, 639, 1230, 1325, 2014
Asp700I	GAANNNNTTC	1 cut(s)	629
Asp718I	GGTACC	1 cut(s)	1736
AspLEI	GCGC	1 cut(s)	1564
AspS9I	GGNCC	5 cut(s)	355, 766, 1582, 1771, 1795
AsuC2I	CCSGG	1 cut(s)	1733
AsuHPI	GGTGA	6 cut(s)	124, 215, 433, 799, 905, 1894
AsuII	TTCGAA	2 cut(s)	2005, 2103
AvaII	GGWCC	3 cut(s)	766, 1771, 1795
BaeGI	GKGCMC	1 cut(s)	1135
BaeI	ACNNNNGTAYC	2 cut(s)	1728, 1761
BamHI	GGATCC	1 cut(s)	430
BanI	GGYRCC	1 cut(s)	1736
BanII	GRGCYC	2 cut(s)	299, 1458
BbsI	GAAGAC	1 cut(s)	455
Bbv12I	GWGCWC	3 cut(s)	1135, 1458, 2035
BbvI	GCAGC	4 cut(s)	1134, 1156, 1518, 1948
BccI	CCATC	9 cut(s)	103, 118, 239, 463, 883, 1011, 1510, 1682, 1843
BceAI	ACGGC	1 cut(s)	1960
BcgI	CGANNNNNNTGC	2 cut(s)	972, 1006
BciT130I	CCWGG	4 cut(s)	359, 1503, 1538, 1667
BciVI	GTATCC	2 cut(s)	699, 1453
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	1 cut(s)	1733
BcoDI	GTCTC	2 cut(s)	222, 670
BcuI	ACTAGT	1 cut(s)	845
BfaI	CTAG	5 cut(s)	171, 551, 846, 1799, 1907
BfoI	RGCGCY	1 cut(s)	1565
BfuAI	ACCTGC	2 cut(s)	1243, 1867
BfuI	GTATCC	2 cut(s)	699, 1453
BglI	GCCNNNNNGGC	1 cut(s)	1859
BisI	GCNGC	4 cut(s)	1145, 1148, 1507, 1962
BlpI	GCTNAGC	1 cut(s)	413
BlsI	GCNGC	4 cut(s)	1146, 1149, 1508, 1963
BmcAI	AGTACT	1 cut(s)	1591
Bme1390I	CCNGG	5 cut(s)	359, 1503, 1538, 1667, 1733
Bme18I	GGWCC	3 cut(s)	766, 1771, 1795
BmgT120I	GGNCC	5 cut(s)	355, 766, 1582, 1771, 1795
BmiI	GGNNCC	4 cut(s)	432, 1542, 1584, 1738
BmrFI	CCNGG	5 cut(s)	359, 1503, 1538, 1667, 1733
BmsI	GCATC	6 cut(s)	1113, 1153, 1175, 1361, 1918, 1952
BpiI	GAAGAC	1 cut(s)	455
Bpu1102I	GCTNAGC	1 cut(s)	413
Bpu14I	TTCGAA	2 cut(s)	2005, 2103
BpuEI	CTTGAG	3 cut(s)	639, 1557, 1578
BpuMI	CCSGG	1 cut(s)	1733
BsaBI	GATNNNNATC	1 cut(s)	435
BsaHI	GRCGYC	1 cut(s)	1728
BsaI	GGTCTC	2 cut(s)	222, 670
Bsc4I	CCNNNNNNNGG	6 cut(s)	83, 245, 598, 775, 871, 1516
Bse118I	RCCGGY	2 cut(s)	1579, 1600
Bse1I	ACTGG	4 cut(s)	279, 1362, 1650, 1911
Bse3DI	GCAATG	1 cut(s)	916
Bse8I	GATNNNNATC	1 cut(s)	435
BseBI	CCWGG	4 cut(s)	359, 1503, 1538, 1667
BseGI	GGATG	8 cut(s)	154, 289, 894, 1104, 1456, 1933, 1943, 2049
BseJI	GATNNNNATC	1 cut(s)	435
BseLI	CCNNNNNNNGG	6 cut(s)	83, 245, 598, 775, 871, 1516
BseMI	GCAATG	1 cut(s)	916
BseMII	CTCAG	4 cut(s)	404, 417, 1461, 1625
BseNI	ACTGG	4 cut(s)	279, 1362, 1650, 1911
BseRI	GAGGAG	2 cut(s)	1286, 2041
BseSI	GKGCMC	1 cut(s)	1135
BseXI	GCAGC	4 cut(s)	1134, 1156, 1518, 1948
BseYI	CCCAGC	1 cut(s)	979
BshFI	GGCC	6 cut(s)	357, 586, 1421, 1583, 1853, 1975
BshNI	GGYRCC	1 cut(s)	1736
BsiHKAI	GWGCWC	3 cut(s)	1135, 1458, 2035
BsiSI	CCGG	4 cut(s)	1422, 1580, 1601, 1732
BslFI	GGGAC	1 cut(s)	828
BslI	CCNNNNNNNGG	6 cut(s)	83, 245, 598, 775, 871, 1516
BsmAI	GTCTC	2 cut(s)	222, 670
BsmFI	GGGAC	1 cut(s)	828
BsmI	GAATGC	1 cut(s)	723
BsnI	GGCC	6 cut(s)	357, 586, 1421, 1583, 1853, 1975
Bso31I	GGTCTC	2 cut(s)	222, 670
Bsp119I	TTCGAA	2 cut(s)	2005, 2103
Bsp1286I	GDGCHC	4 cut(s)	299, 1135, 1458, 2035
Bsp143I	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
Bsp1720I	GCTNAGC	1 cut(s)	413
Bsp19I	CCATGG	2 cut(s)	810, 1740
BspACI	CCGC	1 cut(s)	176
BspANI	GGCC	6 cut(s)	357, 586, 1421, 1583, 1853, 1975
BspCNI	CTCAG	4 cut(s)	405, 418, 1462, 1624
BspLI	GGNNCC	4 cut(s)	432, 1542, 1584, 1738
BspMI	ACCTGC	2 cut(s)	1243, 1867
BspPI	GGATC	4 cut(s)	425, 438, 776, 1053
BspQI	GCTCTTC	1 cut(s)	1380
BspT104I	TTCGAA	2 cut(s)	2005, 2103
BspT107I	GGYRCC	1 cut(s)	1736
BspTNI	GGTCTC	2 cut(s)	222, 670
BsrBI	CCGCTC	1 cut(s)	176
BsrDI	GCAATG	1 cut(s)	916
BsrFI	RCCGGY	2 cut(s)	1579, 1600
BsrI	ACTGG	4 cut(s)	279, 1362, 1650, 1911
BssAI	RCCGGY	2 cut(s)	1579, 1600
BssMI	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
BssNI	GRCGYC	1 cut(s)	1728
BssT1I	CCWWGG	5 cut(s)	810, 1565, 1723, 1740, 1854
Bst2UI	CCWGG	4 cut(s)	359, 1503, 1538, 1667
Bst4CI	ACNGT	5 cut(s)	35, 55, 546, 694, 826
Bst6I	CTCTTC	4 cut(s)	1062, 1232, 1380, 2002
BstACI	GRCGYC	1 cut(s)	1728
BstBI	TTCGAA	2 cut(s)	2005, 2103
BstC8I	GCNNGC	2 cut(s)	1476, 1714
BstDEI	CTNAG	6 cut(s)	413, 426, 1177, 1283, 1470, 1611
BstDSI	CCRYGG	2 cut(s)	810, 1740
BstF5I	GGATG	8 cut(s)	154, 289, 894, 1104, 1456, 1933, 1943, 2049
BstH2I	RGCGCY	1 cut(s)	1565
BstHHI	GCGC	1 cut(s)	1564
BstKTI	GATC	7 cut(s)	262, 433, 784, 928, 1008, 1048, 1486
BstMAI	GTCTC	2 cut(s)	222, 670
BstMBI	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
BstMWI	GCNNNNNNNGC	4 cut(s)	980, 1406, 1687, 1859
BstNI	CCWGG	4 cut(s)	359, 1503, 1538, 1667
BstNSI	RCATGY	2 cut(s)	507, 1186
BstSCI	CCNGG	5 cut(s)	357, 1501, 1536, 1665, 1731
BstSLI	GKGCMC	1 cut(s)	1135
BstV1I	GCAGC	4 cut(s)	1134, 1156, 1518, 1948
BstV2I	GAAGAC	1 cut(s)	455
BstX2I	RGATCY	2 cut(s)	430, 781
BstXI	CCANNNNNNTGG	1 cut(s)	1681
BstYI	RGATCY	2 cut(s)	430, 781
BsuI	GTATCC	2 cut(s)	699, 1453
BsuRI	GGCC	6 cut(s)	357, 586, 1421, 1583, 1853, 1975
BtgI	CCRYGG	2 cut(s)	810, 1740
BtsCI	GGATG	8 cut(s)	154, 289, 894, 1104, 1456, 1933, 1943, 2049
BtsI	GCAGTG	2 cut(s)	1135, 1407
BtsIMutI	CAGTG	4 cut(s)	1135, 1355, 1407, 1918
BveI	ACCTGC	2 cut(s)	1243, 1867
Cac8I	GCNNGC	2 cut(s)	1476, 1714
CfoI	GCGC	1 cut(s)	1564
Cfr10I	RCCGGY	2 cut(s)	1579, 1600
Cfr13I	GGNCC	5 cut(s)	355, 766, 1582, 1771, 1795
CseI	GACGC	1 cut(s)	1717
Csp6I	GTAC	2 cut(s)	1590, 1737
CviQI	GTAC	2 cut(s)	1590, 1737
DdeI	CTNAG	6 cut(s)	413, 426, 1177, 1283, 1470, 1611
DpnI	GATC	7 cut(s)	261, 432, 783, 927, 1007, 1047, 1485
DpnII	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
DraIII	CACNNNGTG	1 cut(s)	1764
EaeI	YGGCCR	1 cut(s)	1973
Eam1104I	CTCTTC	4 cut(s)	1062, 1232, 1380, 2002
EarI	CTCTTC	4 cut(s)	1062, 1232, 1380, 2002
Ecl136II	GAGCTC	1 cut(s)	1456
Eco130I	CCWWGG	5 cut(s)	810, 1565, 1723, 1740, 1854
Eco24I	GRGCYC	2 cut(s)	299, 1458
Eco31I	GGTCTC	2 cut(s)	222, 670
Eco32I	GATATC	1 cut(s)	186
Eco47I	GGWCC	3 cut(s)	766, 1771, 1795
Eco53kI	GAGCTC	1 cut(s)	1456
Eco57I	CTGAAG	2 cut(s)	63, 614
EcoICRI	GAGCTC	1 cut(s)	1456
EcoO109I	RGGNCCY	1 cut(s)	1771
EcoRII	CCWGG	4 cut(s)	357, 1501, 1536, 1665
EcoRV	GATATC	1 cut(s)	186
EcoT14I	CCWWGG	5 cut(s)	810, 1565, 1723, 1740, 1854
EcoT22I	ATGCAT	1 cut(s)	1168
EcoT38I	GRGCYC	2 cut(s)	299, 1458
ErhI	CCWWGG	5 cut(s)	810, 1565, 1723, 1740, 1854
FalI	AAGNNNNNCTT	2 cut(s)	1374, 1406
FaqI	GGGAC	1 cut(s)	828
FauNDI	CATATG	2 cut(s)	1152, 1297
FbaI	TGATCA	1 cut(s)	925
Fnu4HI	GCNGC	4 cut(s)	1145, 1148, 1507, 1962
FokI	GGATG	8 cut(s)	161, 296, 901, 1091, 1463, 1930, 1940, 2056
FriOI	GRGCYC	2 cut(s)	299, 1458
Fsp4HI	GCNGC	4 cut(s)	1145, 1148, 1507, 1962
FspBI	CTAG	5 cut(s)	171, 551, 846, 1799, 1907
GlaI	GCGC	1 cut(s)	1563
GluI	GCNGC	4 cut(s)	1145, 1148, 1507, 1962
GsaI	CCCAGC	1 cut(s)	983
HaeII	RGCGCY	1 cut(s)	1565
HaeIII	GGCC	6 cut(s)	357, 586, 1421, 1583, 1853, 1975
HapII	CCGG	4 cut(s)	1422, 1580, 1601, 1732
HgaI	GACGC	1 cut(s)	1717
HhaI	GCGC	1 cut(s)	1564
Hin1I	GRCGYC	1 cut(s)	1728
Hin6I	GCGC	1 cut(s)	1562
HinP1I	GCGC	1 cut(s)	1562
HindIII	AAGCTT	2 cut(s)	462, 1407
HpaII	CCGG	4 cut(s)	1422, 1580, 1601, 1732
HphI	GGTGA	6 cut(s)	124, 215, 433, 799, 905, 1894
Hpy166II	GTNNAC	3 cut(s)	1133, 1190, 1804
Hpy8I	GTNNAC	3 cut(s)	1133, 1190, 1804
HpyAV	CCTTC	8 cut(s)	87, 217, 589, 654, 748, 754, 1270, 1964
HpyCH4III	ACNGT	5 cut(s)	35, 55, 546, 694, 826
HpyCH4IV	ACGT	1 cut(s)	958
HpyF10VI	GCNNNNNNNGC	4 cut(s)	980, 1406, 1687, 1859
HpyF3I	CTNAG	6 cut(s)	413, 426, 1177, 1283, 1470, 1611
HpySE526I	ACGT	1 cut(s)	958
Hsp92I	GRCGYC	1 cut(s)	1728
HspAI	GCGC	1 cut(s)	1562
KpnI	GGTACC	1 cut(s)	1740
Ksp22I	TGATCA	1 cut(s)	925
Kzo9I	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
LguI	GCTCTTC	1 cut(s)	1380
LmnI	GCTCC	2 cut(s)	241, 1540
Lsp1109I	GCAGC	4 cut(s)	1134, 1156, 1518, 1948
LweI	GCATC	6 cut(s)	1113, 1153, 1175, 1361, 1918, 1952
MaeI	CTAG	5 cut(s)	171, 551, 846, 1799, 1907
MaeII	ACGT	1 cut(s)	958
MaeIII	GTNAC	8 cut(s)	49, 373, 421, 456, 805, 860, 1024, 1465
MalI	GATC	7 cut(s)	261, 432, 783, 927, 1007, 1047, 1485
MbiI	CCGCTC	1 cut(s)	176
MboI	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
MfeI	CAATTG	1 cut(s)	303
MflI	RGATCY	2 cut(s)	430, 781
MhlI	GDGCHC	4 cut(s)	299, 1135, 1458, 2035
MlyI	GAGTC	6 cut(s)	21, 445, 810, 929, 1564, 1603
MmeI	TCCRAC	1 cut(s)	1905
Mph1103I	ATGCAT	1 cut(s)	1168
MroXI	GAANNNNTTC	1 cut(s)	629
MseI	TTAA	6 cut(s)	905, 1248, 1260, 1392, 1497, 1776
MslI	CAYNNNNRTG	5 cut(s)	33, 1109, 1155, 1197, 1877
MspA1I	CMGCKG	1 cut(s)	1147
MspI	CCGG	4 cut(s)	1422, 1580, 1601, 1732
MspR9I	CCNGG	5 cut(s)	359, 1503, 1538, 1667, 1733
MunI	CAATTG	1 cut(s)	303
Mva1269I	GAATGC	1 cut(s)	723
MvaI	CCWGG	4 cut(s)	359, 1503, 1538, 1667
MwoI	GCNNNNNNNGC	4 cut(s)	980, 1406, 1687, 1859
NciI	CCSGG	1 cut(s)	1733
NcoI	CCATGG	2 cut(s)	810, 1740
NdeI	CATATG	2 cut(s)	1152, 1297
NdeII	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
NlaIV	GGNNCC	4 cut(s)	432, 1542, 1584, 1738
NmuCI	GTSAC	4 cut(s)	373, 421, 805, 1465
NsiI	ATGCAT	1 cut(s)	1168
NspI	RCATGY	2 cut(s)	507, 1186
NspV	TTCGAA	2 cut(s)	2005, 2103
PaqCI	CACCTGC	1 cut(s)	1867
PciI	ACATGT	1 cut(s)	1182
PciSI	GCTCTTC	1 cut(s)	1380
PctI	GAATGC	1 cut(s)	723
PdmI	GAANNNNTTC	1 cut(s)	629
PfeI	GAWTC	6 cut(s)	167, 790, 989, 1431, 1491, 2105
PflFI	GACNNNGTC	1 cut(s)	957
PkrI	GCNGC	4 cut(s)	1146, 1149, 1508, 1963
PleI	GAGTC	6 cut(s)	20, 445, 810, 928, 1564, 1603
PpsI	GAGTC	6 cut(s)	20, 445, 810, 928, 1564, 1603
PpuMI	RGGWCCY	1 cut(s)	1771
PscI	ACATGT	1 cut(s)	1182
PsiI	TTATAA	3 cut(s)	1058, 1197, 1622
Psp124BI	GAGCTC	1 cut(s)	1458
Psp5II	RGGWCCY	1 cut(s)	1771
Psp6I	CCWGG	4 cut(s)	357, 1501, 1536, 1665
PspFI	CCCAGC	1 cut(s)	979
PspGI	CCWGG	4 cut(s)	357, 1501, 1536, 1665
PspN4I	GGNNCC	4 cut(s)	432, 1542, 1584, 1738
PspPI	GGNCC	5 cut(s)	355, 766, 1582, 1771, 1795
PspPPI	RGGWCCY	1 cut(s)	1771
PsuI	RGATCY	2 cut(s)	430, 781
PsyI	GACNNNGTC	1 cut(s)	957
PvuII	CAGCTG	1 cut(s)	1147
RsaI	GTAC	2 cut(s)	1591, 1738
RsaNI	GTAC	2 cut(s)	1590, 1737
RseI	CAYNNNNRTG	5 cut(s)	33, 1109, 1155, 1197, 1877
SacI	GAGCTC	1 cut(s)	1458
SapI	GCTCTTC	1 cut(s)	1380
SaqAI	TTAA	6 cut(s)	905, 1248, 1260, 1392, 1497, 1776
SatI	GCNGC	4 cut(s)	1145, 1148, 1507, 1962
Sau3AI	GATC	7 cut(s)	259, 430, 781, 925, 1005, 1045, 1483
Sau96I	GGNCC	5 cut(s)	355, 766, 1582, 1771, 1795
ScaI	AGTACT	1 cut(s)	1591
SchI	GAGTC	6 cut(s)	21, 445, 810, 929, 1564, 1603
ScrFI	CCNGG	5 cut(s)	359, 1503, 1538, 1667, 1733
SduI	GDGCHC	4 cut(s)	299, 1135, 1458, 2035
SfaNI	GCATC	6 cut(s)	1113, 1153, 1175, 1361, 1918, 1952
SfuI	TTCGAA	2 cut(s)	2005, 2103
SinI	GGWCC	3 cut(s)	766, 1771, 1795
SmiMI	CAYNNNNRTG	5 cut(s)	33, 1109, 1155, 1197, 1877
SmlI	CTYRAG	3 cut(s)	654, 1572, 1593
SmoI	CTYRAG	3 cut(s)	654, 1572, 1593
SpeI	ACTAGT	1 cut(s)	845
SsiI	CCGC	1 cut(s)	176
SspI	AATATT	1 cut(s)	1753
SspMI	CTAG	5 cut(s)	171, 551, 846, 1799, 1907
SstI	GAGCTC	1 cut(s)	1458
StyD4I	CCNGG	5 cut(s)	357, 1501, 1536, 1665, 1731
StyI	CCWWGG	5 cut(s)	810, 1565, 1723, 1740, 1854
TaaI	ACNGT	5 cut(s)	35, 55, 546, 694, 826
TaiI	ACGT	1 cut(s)	961
TaqI	TCGA	6 cut(s)	364, 390, 633, 1004, 2005, 2103
TaqII	GACCGA	1 cut(s)	1303
TatI	WGTACW	1 cut(s)	1589
TfiI	GAWTC	6 cut(s)	167, 790, 989, 1431, 1491, 2105
Tru1I	TTAA	6 cut(s)	905, 1248, 1260, 1392, 1497, 1776
Tru9I	TTAA	6 cut(s)	905, 1248, 1260, 1392, 1497, 1776
TscAI	CASTG	4 cut(s)	1135, 1362, 1407, 1918
TseFI	GTSAC	4 cut(s)	373, 421, 805, 1465
TseI	GCWGC	4 cut(s)	1144, 1147, 1506, 1961
Tsp45I	GTSAC	4 cut(s)	373, 421, 805, 1465
TspGWI	ACGGA	1 cut(s)	1621
TspRI	CASTG	4 cut(s)	1135, 1362, 1407, 1918
Tth111I	GACNNNGTC	1 cut(s)	957
VneI	GTGCAC	1 cut(s)	1131
VpaK11BI	GGWCC	3 cut(s)	766, 1771, 1795
XapI	RAATTY	5 cut(s)	137, 639, 1230, 1325, 2014
XbaI	TCTAGA	1 cut(s)	170
XceI	RCATGY	2 cut(s)	507, 1186
XmnI	GAANNNNTTC	1 cut(s)	629
XspI	CTAG	5 cut(s)	171, 551, 846, 1799, 1907
ZrmI	AGTACT	1 cut(s)	1591
Zsp2I	ATGCAT	1 cut(s)	1168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.