Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Reverse (-)

3461656 .. 3464571

Gene Structure 2916 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g04700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2814 bp

ATGGCCCTCGAGATAAATATAGAGGATCAAGCTGCTGAAGGTGCCTCTCTTACTGCCGGGTTGACTATGCAGAGCATCAGACGCAGGAGAACCTCGCTGAGATATGTTGTTAAAGGCATTTTGTTCTCATCACGCATTAAGAAAACCAGACGTCTGCTTGAAGCTAGTTATGAACATTTAGCACCAGCACCAGCGCCGCCAGCCACTGCCTCTGACCATGTGATTGACATCCAGGAATGGCTTTCGAAAGAGGTTGCTGAAATAGTGAGGGAGAAGGACTTGAACTCACTCTGCGGACATGGTGGTGTTGATGGCATCTTCCGACTTCTCACGTCTCACTTTGAGGAAGGTGCCGTTGATGGTGGTCAAAATCCAGAAGGATGGAACATTACCAAGTCCCCTGTTGATGCAAAGGGTTTCTTGTACTTTTTGTTTGAGGCGTTCAATCAGTACACGATTGTTTTCCTCTTGCTCTCAGCTGGGTTATCGTTCACCATCGAATTCATGAATCAAGGAGTCAAGGATGGCTGGCATGACGGTGTTGCCATACTCACTGCCGCGTTCTTACTTATTGCTTTCCGTTCTGTGGGGAACTACCTCCGTCAGAGAAAGCTGGCGAAGCACTTGCTGGATAGGAGCCAATTGACGGTGAATGTTGAAAGAAGTAGCACAGAACCTACACCGATTACCATATCCGACATTGTGGTGGGTGATATAGTTCTTTTGAAGGAAGGAGACTGTGTTCCTGCTGATGGTTTGTTCATAGATCACGGTGAGGAACTGGTTCTGGATGAGGTATTGAGCCCGAAAATTGATTGTAAGCAAAATCCATTTGTTTTTTCGGGTTCAAAGGTCGTTAAGGGCGGTGGCCGCATGATTGTGACATCAGTTGGTGCTAATACAGCTATGGCCGAGATGCTGAGCTTGGTGTCTAATCATAATCCAATGGAAAAGACACTGCTACAAGCTTTGCTGGACAAACCGAATGCTTGTATGGATTTTCTTGCATGCTGTGTCTCGATACTGATTGCACTTGTGATGTTGATACGCCTACTATTCTTCAAGAAGCACGAAAACTACAACGAGAGTCCAGACCTGAAAGGTGAAGTTTCAATTAATTTTGTGATGAGGATCTTTGAGAAGATCTTCTTGAAACCTCAAGGAAAGGCTTCCATCTTAGCAAGCGTTCTCGCAACCGTGGTCATAGGGATACAACATGGGATACCTGTTGTAATTACAGTTGCCCTTTTTAAATGGAAGGACAAGGTTGCCAAAAATCAGGCAGATCCACGGAATCTATCAGCTTGTGTCACCATGGGACTCATAAATTTCATCTGCATTGAAACAACGGCAGATTTAATGTGCAACCGGACGGAGGTCGAGGAATTTCGGATTGGCGAAAAAGATTTAAGCAGTGGTGAAGTGGACTTGGAAACTGACCGAGTTCTTGAGGCTCTCCATCAGGGGATTTCAGCTACCGCGTCACCAGAAAATGATCGGCTCATTTCTTGGCTGAACACCAGACGGGTAAGTAAAGCGTTATTGGATCGAAGATTTGAAATTGTTAAACAATGGAGCTCTAATGAGAAGTATAGAGGTGTCGTATTGAGGAAAATCGAGAATGATGAACAAATTATGCAACTTCACTGTGACGGAGAAGCATCAACAATATTAGGCATGTGTTCACATTACTATGATAACAGAGGGGGAAGTCTCCATATTGGAAACCAGAAGAGAAAATTCAAGCAGGTGATCGAGAAGATGGAGAAGAAGGGTCTTAGACCGATTGCATACGCATACAAGAAAACGAAAGAAGAAGATCCTACAGAAGATGATTTGATTTTGTTGGCACTTGTCGGTCTTAGATGTCCATATCAAGAAGAACTTAAATTGGCGGTGGAGACTCTTAGAGAAGCTGGAGTAAAGATCACACTGGTGTCGGAGGATGAGCTCTCGAAAGTGAAAGCTAGAGCTAGGGAGCTTGGGATCTCCCCTGACTCAGATTACACAGAAATTGAAGGTCAAGATTTCCGAAGGCTCAAATCCATGGAAAGGCGGGAGAAGGTGGATTCGATCTCTGTGATGGGAAGTTCCCTCCCCAAGGACAAGTTTCTTCTGGTGGAGCAGTTAAAGAAAAAAGGTCACATAGTTGGATTCTATGGAGGGCTAACCAGCATTGACGCTCTGACCTTAAAAACAGCTGATGTAGGAATTGTAGAGTATCCCTGGAGCACCGAGATGGCCAGAGAGAACGCTGATCTTATAATCAGAAATGGATGTTTCTTAAGCAAGAATATGAAGTCTGGTGCTTGTGCTTACCACAACATTCAGCAGTTCTGTCAACTTCAACTCACTACTTGCATATCCGGGCTACTATTGACCCTAGTCGTAACAATGCATTCGGGAGAGTCCCCACTAACAACAGTTCACTTGATTTGGGTGAACTTGATCATGTGCCTTCTTGGTGGCCTAATGATGGTGATGGAGTTACAAGGCACCGAACGACACACTCAAAGACCTGCTAAGAGGACCGAGTGGCTTATAACCAAAGTCATCTGGGGAAACATAGCAGTTCAAGTCTTGTATCAGGCTTCCGTCTTGCTGATCTTACATTTCATGGGAAATGTAGTACCGAGCATGAACGAAGGTGTCCGGAATACCATGATTTTCAATACTTTCACCTTATGCCAGGTCCTTAATCTGTTCAGTGCCATGGACATAGTAAAAAAGGAAGTGCTAGTTGTTGTCCTCCGCAGCCATTGGTTTCTGACGGCGTTGGCGGCTGTTTTGATCGATCTAAGTGATCATTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

938

Amino Acids

104.57

Weight (kDa)

6.46

Isoelectric Point (pI)

39.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	216 - 310	3.3e-11	P-type ATPase actuator domain
Cation_ATPase	PF13246	515 - 627	1.1e-09	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	804 - 930	1.1e-18	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2264, 2543
AarI	CACCTGC	1 cut(s)	1738
AatII	GACGTC	1 cut(s)	154
Acc36I	ACCTGC	2 cut(s)	1738, 2527
AccB1I	GGYRCC	3 cut(s)	41, 350, 2495
AccII	CGCG	2 cut(s)	560, 1481
AccIII	TCCGGA	1 cut(s)	2652
AclWI	GGATC	6 cut(s)	33, 1139, 1280, 1554, 1814, 1994
AcoI	YGGCCR	3 cut(s)	868, 909, 2241
AcsI	RAATTY	4 cut(s)	500, 1327, 1385, 1739
AcuI	CTGAAG	1 cut(s)	57
AcyI	GRCGYC	1 cut(s)	151
AfaI	GTAC	3 cut(s)	425, 452, 2631
AfiI	CCNNNNNNNGG	7 cut(s)	586, 646, 752, 1375, 2052, 2101, 2476
AflII	CTTAAG	1 cut(s)	2284
AjiI	CACGTC	1 cut(s)	333
AjnI	CCWGG	3 cut(s)	231, 2225, 2688
AjuI	GAANNNNNNNTTGG	2 cut(s)	1377, 1409
AleI	CACNNNNGTG	1 cut(s)	1934
AloI	GAACNNNNNNTCC	2 cut(s)	726, 758
Alw21I	GWGCWC	3 cut(s)	1580, 1953, 2234
Alw26I	GTCTC	5 cut(s)	339, 729, 1021, 1718, 1895
AlwI	GGATC	6 cut(s)	33, 1139, 1280, 1554, 1814, 1994
AlwNI	CAGNNNCTG	1 cut(s)	206
Ama87I	CYCGRG	1 cut(s)	8
Aor13HI	TCCGGA	1 cut(s)	2652
AoxI	GGCC	5 cut(s)	3, 868, 909, 2241, 2467
ApeKI	GCWGC	2 cut(s)	32, 2754
ApoI	RAATTY	4 cut(s)	500, 1327, 1385, 1739
AseI	ATTAAT	1 cut(s)	1116
Asp700I	GAANNNNTTC	3 cut(s)	783, 1145, 1168
AspLEI	GCGC	1 cut(s)	196
AspS9I	GGNCC	3 cut(s)	4, 2529, 2692
AsuC2I	CCSGG	2 cut(s)	58, 2368
AsuII	TTCGAA	1 cut(s)	245
AvaI	CYCGRG	1 cut(s)	8
AvaII	GGWCC	2 cut(s)	2529, 2692
BalI	TGGCCA	1 cut(s)	2243
BanI	GGYRCC	3 cut(s)	41, 350, 2495
BanII	GRGCYC	3 cut(s)	806, 1580, 1953
Bbv12I	GWGCWC	3 cut(s)	1580, 1953, 2234
BbvI	GCAGC	2 cut(s)	19, 2766
BceAI	ACGGC	3 cut(s)	338, 1365, 2787
BcgI	CGANNNNNNTGC	2 cut(s)	607, 641
BciT130I	CCWGG	3 cut(s)	233, 2227, 2690
BciVI	GTATCC	3 cut(s)	1203, 1215, 2232
BclI	TGATCA	2 cut(s)	2448, 2803
BcnI	CCSGG	2 cut(s)	58, 2368
BcoDI	GTCTC	5 cut(s)	339, 729, 1021, 1718, 1895
BfaI	CTAG	5 cut(s)	165, 1968, 1974, 2384, 2738
BfmI	CTRYAG	1 cut(s)	1824
BfoI	RGCGCY	1 cut(s)	197
BfrI	CTTAAG	1 cut(s)	2284
BfuAI	ACCTGC	2 cut(s)	1738, 2527
BfuI	GTATCC	3 cut(s)	1203, 1215, 2232
BglII	AGATCT	1 cut(s)	1143
BisI	GCNGC	6 cut(s)	33, 197, 558, 871, 2755, 2781
BlpI	GCTNAGC	1 cut(s)	920
BlsI	GCNGC	6 cut(s)	34, 198, 559, 872, 2756, 2782
Bme1390I	CCNGG	5 cut(s)	58, 233, 2227, 2368, 2690
Bme18I	GGWCC	2 cut(s)	2529, 2692
BmeT110I	CYCGRG	1 cut(s)	8
BmgBI	CACGTC	1 cut(s)	333
BmgT120I	GGNCC	3 cut(s)	4, 2529, 2692
BmiI	GGNNCC	4 cut(s)	43, 352, 638, 2497
BmrFI	CCNGG	5 cut(s)	58, 233, 2227, 2368, 2690
BmsI	GCATC	5 cut(s)	84, 324, 397, 906, 1670
BoxI	GACNNNNGTC	2 cut(s)	1376, 2384
BpmI	CTGGAG	2 cut(s)	1938, 2248
Bpu1102I	GCTNAGC	1 cut(s)	920
Bpu14I	TTCGAA	1 cut(s)	245
BpuEI	CTTGAG	2 cut(s)	1143, 1469
BpuMI	CCSGG	2 cut(s)	58, 2368
Bsa29I	ATCGAT	1 cut(s)	2793
BsaBI	GATNNNNATC	2 cut(s)	227, 1130
BsaHI	GRCGYC	1 cut(s)	151
BsaJI	CCNNGG	7 cut(s)	1197, 1289, 1314, 2046, 2100, 2225, 2712
BsaWI	WCCGGW	2 cut(s)	1368, 2652
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	7 cut(s)	586, 646, 752, 1375, 2052, 2101, 2476
Bse1I	ACTGG	2 cut(s)	786, 1938
Bse8I	GATNNNNATC	2 cut(s)	227, 1130
BseAI	TCCGGA	1 cut(s)	2652
BseBI	CCWGG	3 cut(s)	233, 2227, 2690
BseCI	ATCGAT	1 cut(s)	2793
BseDI	CCNNGG	7 cut(s)	1197, 1289, 1314, 2046, 2100, 2225, 2712
BseGI	GGATG	6 cut(s)	228, 386, 529, 796, 1951, 2282
BseJI	GATNNNNATC	2 cut(s)	227, 1130
BseLI	CCNNNNNNNGG	7 cut(s)	586, 646, 752, 1375, 2052, 2101, 2476
BseMII	CTCAG	4 cut(s)	89, 489, 911, 2013
BseNI	ACTGG	2 cut(s)	786, 1938
BseXI	GCAGC	2 cut(s)	19, 2766
BseYI	CCCAGC	1 cut(s)	479
Bsh1236I	CGCG	2 cut(s)	560, 1481
BshFI	GGCC	5 cut(s)	5, 870, 911, 2243, 2469
BshNI	GGYRCC	3 cut(s)	41, 350, 2495
BshVI	ATCGAT	1 cut(s)	2793
BsiHKAI	GWGCWC	3 cut(s)	1580, 1953, 2234
BsiHKCI	CYCGRG	1 cut(s)	8
BsiSI	CCGG	4 cut(s)	57, 1369, 2367, 2653
BslFI	GGGAC	3 cut(s)	382, 1332, 2395
BslI	CCNNNNNNNGG	7 cut(s)	586, 646, 752, 1375, 2052, 2101, 2476
BsmAI	GTCTC	5 cut(s)	339, 729, 1021, 1718, 1895
BsmBI	CGTCTC	1 cut(s)	339
BsmFI	GGGAC	3 cut(s)	382, 1332, 2395
BsmI	GAATGC	2 cut(s)	991, 2398
BsnI	GGCC	5 cut(s)	5, 870, 911, 2243, 2469
BsoBI	CYCGRG	1 cut(s)	8
Bsp119I	TTCGAA	1 cut(s)	245
Bsp1286I	GDGCHC	4 cut(s)	806, 1580, 1953, 2234
Bsp13I	TCCGGA	1 cut(s)	2652
Bsp1720I	GCTNAGC	1 cut(s)	920
Bsp19I	CCATGG	3 cut(s)	1314, 2046, 2712
BspANI	GGCC	5 cut(s)	5, 870, 911, 2243, 2469
BspCNI	CTCAG	4 cut(s)	90, 488, 912, 2012
BspDI	ATCGAT	1 cut(s)	2793
BspEI	TCCGGA	1 cut(s)	2652
BspFNI	CGCG	2 cut(s)	560, 1481
BspHI	TCATGA	1 cut(s)	504
BspLI	GGNNCC	4 cut(s)	43, 352, 638, 2497
BspMI	ACCTGC	2 cut(s)	1738, 2527
BspPI	GGATC	6 cut(s)	33, 1139, 1280, 1554, 1814, 1994
BspT104I	TTCGAA	1 cut(s)	245
BspT107I	GGYRCC	3 cut(s)	41, 350, 2495
BspTI	CTTAAG	1 cut(s)	2284
BsrI	ACTGG	2 cut(s)	786, 1938
BssECI	CCNNGG	7 cut(s)	1197, 1289, 1314, 2046, 2100, 2225, 2712
BssNI	GRCGYC	1 cut(s)	151
BssT1I	CCWWGG	4 cut(s)	1314, 2046, 2100, 2712
Bst2UI	CCWGG	3 cut(s)	233, 2227, 2690
Bst4CI	ACNGT	8 cut(s)	539, 649, 740, 773, 1198, 1240, 1649, 2425
Bst6I	CTCTTC	1 cut(s)	1727
BstACI	GRCGYC	1 cut(s)	151
BstAFI	CTTAAG	1 cut(s)	2284
BstBI	TTCGAA	1 cut(s)	245
BstC8I	GCNNGC	5 cut(s)	201, 530, 615, 1009, 1183
BstDSI	CCRYGG	5 cut(s)	1197, 1289, 1314, 2046, 2712
BstENI	CCTNNNNNAGG	1 cut(s)	2099
BstF5I	GGATG	6 cut(s)	228, 386, 529, 796, 1951, 2282
BstFNI	CGCG	2 cut(s)	560, 1481
BstH2I	RGCGCY	1 cut(s)	197
BstHHI	GCGC	1 cut(s)	196
BstMAI	GTCTC	5 cut(s)	339, 729, 1021, 1718, 1895
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 81, 200, 619, 870, 902, 2780
BstNI	CCWGG	3 cut(s)	233, 2227, 2690
BstNSI	RCATGY	2 cut(s)	1011, 1681
BstPAI	GACNNNNGTC	2 cut(s)	1376, 2384
BstSCI	CCNGG	5 cut(s)	56, 231, 2225, 2366, 2688
BstSFI	CTRYAG	1 cut(s)	1824
BstUI	CGCG	2 cut(s)	560, 1481
BstV1I	GCAGC	2 cut(s)	19, 2766
BstX2I	RGATCY	5 cut(s)	1131, 1143, 1285, 1819, 1986
BstXI	CCANNNNNNTGG	1 cut(s)	381
BstYI	RGATCY	5 cut(s)	1131, 1143, 1285, 1819, 1986
Bsu15I	ATCGAT	1 cut(s)	2793
BsuI	GTATCC	3 cut(s)	1203, 1215, 2232
BsuRI	GGCC	5 cut(s)	5, 870, 911, 2243, 2469
BsuTUI	ATCGAT	1 cut(s)	2793
BtgI	CCRYGG	5 cut(s)	1197, 1289, 1314, 2046, 2712
BtrI	CACGTC	1 cut(s)	333
BtsCI	GGATG	6 cut(s)	228, 386, 529, 796, 1951, 2282
BtsI	GCAGTG	4 cut(s)	204, 552, 956, 1420
BtsIMutI	CAGTG	7 cut(s)	204, 552, 956, 1420, 1645, 1931, 2713
BveI	ACCTGC	2 cut(s)	1738, 2527
Cac8I	GCNNGC	5 cut(s)	201, 530, 615, 1009, 1183
CaiI	CAGNNNCTG	1 cut(s)	206
CciI	TCATGA	1 cut(s)	504
CfoI	GCGC	1 cut(s)	196
Cfr13I	GGNCC	3 cut(s)	4, 2529, 2692
ClaI	ATCGAT	1 cut(s)	2793
CseI	GACGC	3 cut(s)	90, 1470, 2189
Csp6I	GTAC	3 cut(s)	424, 451, 2630
CviQI	GTAC	3 cut(s)	424, 451, 2630
DraI	TTTAAA	1 cut(s)	1252
EaeI	YGGCCR	3 cut(s)	868, 909, 2241
Eam1104I	CTCTTC	1 cut(s)	1727
EarI	CTCTTC	1 cut(s)	1727
Ecl136II	GAGCTC	2 cut(s)	1578, 1951
Eco130I	CCWWGG	4 cut(s)	1314, 2046, 2100, 2712
Eco24I	GRGCYC	3 cut(s)	806, 1580, 1953
Eco47I	GGWCC	2 cut(s)	2529, 2692
Eco53kI	GAGCTC	2 cut(s)	1578, 1951
Eco57I	CTGAAG	1 cut(s)	57
Eco88I	CYCGRG	1 cut(s)	8
EcoICRI	GAGCTC	2 cut(s)	1578, 1951
EcoNI	CCTNNNNNAGG	1 cut(s)	2099
EcoO109I	RGGNCCY	1 cut(s)	2692
EcoRI	GAATTC	1 cut(s)	500
EcoRII	CCWGG	3 cut(s)	231, 2225, 2688
EcoT14I	CCWWGG	4 cut(s)	1314, 2046, 2100, 2712
EcoT22I	ATGCAT	1 cut(s)	2400
EcoT38I	GRGCYC	3 cut(s)	806, 1580, 1953
ErhI	CCWWGG	4 cut(s)	1314, 2046, 2100, 2712
Esp3I	CGTCTC	1 cut(s)	339
FalI	AAGNNNNNCTT	6 cut(s)	404, 436, 1133, 1165, 1869, 1901
FaqI	GGGAC	3 cut(s)	382, 1332, 2395
FauI	CCCGC	1 cut(s)	2049
FbaI	TGATCA	2 cut(s)	2448, 2803
Fnu4HI	GCNGC	6 cut(s)	33, 197, 558, 871, 2755, 2781
FokI	GGATG	6 cut(s)	215, 393, 536, 803, 1958, 2289
FriOI	GRGCYC	3 cut(s)	806, 1580, 1953
Fsp4HI	GCNGC	6 cut(s)	33, 197, 558, 871, 2755, 2781
FspBI	CTAG	5 cut(s)	165, 1968, 1974, 2384, 2738
GlaI	GCGC	1 cut(s)	195
GluI	GCNGC	6 cut(s)	33, 197, 558, 871, 2755, 2781
GsaI	CCCAGC	1 cut(s)	483
GsuI	CTGGAG	2 cut(s)	1938, 2248
HaeII	RGCGCY	1 cut(s)	197
HaeIII	GGCC	5 cut(s)	5, 870, 911, 2243, 2469
HapII	CCGG	4 cut(s)	57, 1369, 2367, 2653
HgaI	GACGC	3 cut(s)	90, 1470, 2189
HhaI	GCGC	1 cut(s)	196
Hin1I	GRCGYC	1 cut(s)	151
Hin6I	GCGC	1 cut(s)	194
HinP1I	GCGC	1 cut(s)	194
HincII	GTYRAC	2 cut(s)	63, 2342
HindII	GTYRAC	2 cut(s)	63, 2342
HindIII	AAGCTT	1 cut(s)	966
HpaII	CCGG	4 cut(s)	57, 1369, 2367, 2653
Hpy166II	GTNNAC	8 cut(s)	63, 453, 492, 1426, 1685, 2342, 2428, 2443
Hpy8I	GTNNAC	8 cut(s)	63, 453, 492, 1426, 1685, 2342, 2428, 2443
HpyCH4III	ACNGT	8 cut(s)	539, 649, 740, 773, 1198, 1240, 1649, 2425
HpyCH4IV	ACGT	2 cut(s)	151, 332
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 81, 200, 619, 870, 902, 2780
HpySE526I	ACGT	2 cut(s)	151, 332
Hsp92I	GRCGYC	1 cut(s)	151
HspAI	GCGC	1 cut(s)	194
Kpn2I	TCCGGA	1 cut(s)	2652
Ksp22I	TGATCA	2 cut(s)	2448, 2803
LmnI	GCTCC	5 cut(s)	636, 1575, 1978, 2122, 2229
Lsp1109I	GCAGC	2 cut(s)	19, 2766
LweI	GCATC	5 cut(s)	84, 324, 397, 906, 1670
MaeI	CTAG	5 cut(s)	165, 1968, 1974, 2384, 2738
MaeII	ACGT	2 cut(s)	151, 332
MaeIII	GTNAC	7 cut(s)	880, 1309, 1482, 1649, 2141, 2389, 2487
MfeI	CAATTG	1 cut(s)	641
MflI	RGATCY	5 cut(s)	1131, 1143, 1285, 1819, 1986
MhlI	GDGCHC	4 cut(s)	806, 1580, 1953, 2234
MlsI	TGGCCA	1 cut(s)	2243
MluNI	TGGCCA	1 cut(s)	2243
MlyI	GAGTC	6 cut(s)	525, 1096, 1314, 1897, 1991, 2417
MmeI	TCCRAC	4 cut(s)	346, 720, 1920, 2131
Mox20I	TGGCCA	1 cut(s)	2243
Mph1103I	ATGCAT	1 cut(s)	2400
MroI	TCCGGA	1 cut(s)	2652
MroXI	GAANNNNTTC	3 cut(s)	783, 1145, 1168
MscI	TGGCCA	1 cut(s)	2243
MslI	CAYNNNNRTG	7 cut(s)	303, 537, 704, 878, 1692, 1934, 2237
Msp20I	TGGCCA	1 cut(s)	2243
MspA1I	CMGCKG	2 cut(s)	479, 2201
MspCI	CTTAAG	1 cut(s)	2284
MspI	CCGG	4 cut(s)	57, 1369, 2367, 2653
MspR9I	CCNGG	5 cut(s)	58, 233, 2227, 2368, 2690
MunI	CAATTG	1 cut(s)	641
Mva1269I	GAATGC	2 cut(s)	991, 2398
MvaI	CCWGG	3 cut(s)	233, 2227, 2690
MvnI	CGCG	2 cut(s)	560, 1481
MwoI	GCNNNNNNNGC	7 cut(s)	41, 81, 200, 619, 870, 902, 2780
NciI	CCSGG	2 cut(s)	58, 2368
NcoI	CCATGG	3 cut(s)	1314, 2046, 2712
NlaIV	GGNNCC	4 cut(s)	43, 352, 638, 2497
NmeAIII	GCCGAG	1 cut(s)	937
NmuCI	GTSAC	5 cut(s)	880, 1309, 1482, 1649, 2141
NsiI	ATGCAT	1 cut(s)	2400
NspI	RCATGY	2 cut(s)	1011, 1681
NspV	TTCGAA	1 cut(s)	245
OliI	CACNNNNGTG	1 cut(s)	1934
PaeI	GCATGC	1 cut(s)	1011
PaeR7I	CTCGAG	1 cut(s)	8
PagI	TCATGA	1 cut(s)	504
PaqCI	CACCTGC	1 cut(s)	1738
PctI	GAATGC	2 cut(s)	991, 2398
PdmI	GAANNNNTTC	3 cut(s)	783, 1145, 1168
PfeI	GAWTC	4 cut(s)	508, 1294, 2069, 2154
PfoI	TCCNGGA	1 cut(s)	231
PkrI	GCNGC	6 cut(s)	34, 198, 559, 872, 2756, 2782
PleI	GAGTC	6 cut(s)	524, 1095, 1314, 1897, 1991, 2416
PpsI	GAGTC	6 cut(s)	524, 1095, 1314, 1897, 1991, 2416
PpuMI	RGGWCCY	1 cut(s)	2692
PshAI	GACNNNNGTC	2 cut(s)	1376, 2384
PshBI	ATTAAT	1 cut(s)	1116
PsiI	TTATAA	2 cut(s)	2264, 2543
Psp124BI	GAGCTC	2 cut(s)	1580, 1953
Psp5II	RGGWCCY	1 cut(s)	2692
Psp6I	CCWGG	3 cut(s)	231, 2225, 2688
PspFI	CCCAGC	1 cut(s)	479
PspGI	CCWGG	3 cut(s)	231, 2225, 2688
PspN4I	GGNNCC	4 cut(s)	43, 352, 638, 2497
PspPI	GGNCC	3 cut(s)	4, 2529, 2692
PspPPI	RGGWCCY	1 cut(s)	2692
PstNI	CAGNNNCTG	1 cut(s)	206
PsuI	RGATCY	5 cut(s)	1131, 1143, 1285, 1819, 1986
PvuII	CAGCTG	2 cut(s)	479, 2201
RsaI	GTAC	3 cut(s)	425, 452, 2631
RsaNI	GTAC	3 cut(s)	424, 451, 2630
RseI	CAYNNNNRTG	7 cut(s)	303, 537, 704, 878, 1692, 1934, 2237
SacI	GAGCTC	2 cut(s)	1580, 1953
SatI	GCNGC	6 cut(s)	33, 197, 558, 871, 2755, 2781
Sau96I	GGNCC	3 cut(s)	4, 2529, 2692
SchI	GAGTC	6 cut(s)	525, 1096, 1314, 1897, 1991, 2417
ScrFI	CCNGG	5 cut(s)	58, 233, 2227, 2368, 2690
SduI	GDGCHC	4 cut(s)	806, 1580, 1953, 2234
SfaNI	GCATC	5 cut(s)	84, 324, 397, 906, 1670
SfcI	CTRYAG	1 cut(s)	1824
Sfr274I	CTCGAG	1 cut(s)	8
SfuI	TTCGAA	1 cut(s)	245
SinI	GGWCC	2 cut(s)	2529, 2692
SlaI	CTCGAG	1 cut(s)	8
SmiMI	CAYNNNNRTG	7 cut(s)	303, 537, 704, 878, 1692, 1934, 2237
SmlI	CTYRAG	4 cut(s)	8, 1158, 1448, 2284
SmoI	CTYRAG	4 cut(s)	8, 1158, 1448, 2284
SphI	GCATGC	1 cut(s)	1011
SspI	AATATT	1 cut(s)	1671
SspMI	CTAG	5 cut(s)	165, 1968, 1974, 2384, 2738
SstI	GAGCTC	2 cut(s)	1580, 1953
StyD4I	CCNGG	5 cut(s)	56, 231, 2225, 2366, 2688
StyI	CCWWGG	4 cut(s)	1314, 2046, 2100, 2712
TaaI	ACNGT	8 cut(s)	539, 649, 740, 773, 1198, 1240, 1649, 2425
TaiI	ACGT	2 cut(s)	154, 335
TaqII	GACCGA	4 cut(s)	1455, 1798, 1847, 2546
TatI	WGTACW	2 cut(s)	423, 450
TauI	GCSGC	4 cut(s)	199, 560, 873, 2783
TfiI	GAWTC	4 cut(s)	508, 1294, 2069, 2154
TscAI	CASTG	7 cut(s)	211, 559, 963, 1420, 1652, 1938, 2713
TseFI	GTSAC	5 cut(s)	880, 1309, 1482, 1649, 2141
TseI	GCWGC	2 cut(s)	32, 2754
Tsp45I	GTSAC	5 cut(s)	880, 1309, 1482, 1649, 2141
TspDTI	ATGAA	9 cut(s)	186, 493, 521, 751, 1321, 1641, 2312, 2605, 2654
TspGWI	ACGGA	6 cut(s)	569, 590, 1306, 1388, 1668, 2584
TspRI	CASTG	7 cut(s)	211, 559, 963, 1420, 1652, 1938, 2713
Vha464I	CTTAAG	1 cut(s)	2284
VpaK11BI	GGWCC	2 cut(s)	2529, 2692
VspI	ATTAAT	1 cut(s)	1116
XagI	CCTNNNNNAGG	1 cut(s)	2099
XapI	RAATTY	4 cut(s)	500, 1327, 1385, 1739
XceI	RCATGY	2 cut(s)	1011, 1681
XhoI	CTCGAG	1 cut(s)	8
XmnI	GAANNNNTTC	3 cut(s)	783, 1145, 1168
XspI	CTAG	5 cut(s)	165, 1968, 1974, 2384, 2738
ZraI	GACGTC	1 cut(s)	152
Zsp2I	ATGCAT	1 cut(s)	2400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.