Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

74176679 .. 74180713

Gene Structure 4035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG532600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGAAGCAAGGAGTCAAACAAGGGTGGCATGACGGTGTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTTCCTTCAGTTGGGAACTATCGCAACGAGAGGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTTCTGGTGGATGTTGAAAGAAATGGAGAATCTAGAGCGGTTATGATATCTCATGTTTCGGTGGGTGATATAGTTCATTTGAAGAAGGGAGACCATGCTCCTGCTGATGGCTTGTTCATAGATCATGGTGTGGAACTGGTCTTGGATGAGGTATTGAGCCCCACAATTGATTTTGAAAACAACCCATTTGTGTTGTCTGGTTCAAAAGTTATTGAGGGCCAGGGTCGAATGATTGTGACATCTGTTGGTGTCGATACAGCTTTTGCAGATATGCTGAGCTTGGTGACTGAGGATCCTAATCCAAATAGGAAGACTCTGTTACAAGCTTTGATGGACAAACCATATACTTATATGGAGTATCTCGCATGTTGTGTTTCAATTCTGATTGCTTTTGTGGTGCTAATACGGTTTCTAGTATTCAGGAAGCATGACAACTATAATGACAGGCCAGACCTGAAGGGGGAAGTTTCAATGAACTTGCTGATGAAGATTTTCGAGAAAATTTACTTGAAAACTCAAGGAAGGGTCTCCACTTTAGCAAGTGTTCTTGCTACTGTGGTAATTGGGATACAACATGGAATGCCCTTTGTGATTTCCCTTGCACTTTGTCAATGGAAGGAGAAGGTGGTCCAAAATGGGGCAGATCCTCGGAATCTTTCAGCTTGTGTCACCATGGGACTCATAACAGTCATCTGCATTGAAACAACTAGTGAGAAAATGTGTAACCAGATAGAGGTTATGGACTTTTGGATGGGTGATAAGGATTTAAGCAATGAAGTGGAGTCTGATCAAACTGAACAATTTGCTCTCAAAGGACTACGTCAAGAGATTTCTGCTACCCCAGCAAATGATTCGTTCATTTCTTCGATCAAGACCAGATGGGGCGTTACTGACATGGAGGTATTGGATCAGACATTTATAACTCTGGAAGAGAGAAAATTGAGTTCTGATGAGAAATGTAGTGGCATCCTAATGAGGAAAACAGAGAGCAGTGCACAAGATATGCAGCTGCATATGAGTGGAGATGCATCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACAGGGGAAAGTCGTATTCTGGAAATTCAGAAGAGAAAGTTTAAGCAGGTCATTAAGAACATGGAGGAGAAGGGTCTTAGACCGATTGCATATGCTTACAAGAAAACAAATGTTCAAGAATTTACAGAAAATGGGTTGAAATTGTTGGCACTGGTGGGGGTCAGATGCCCATATCAAGAAGAGCTTAAAATTGCAGTGGAAGCTTTTAGAAAGGCCGGAATAAGAATCAAGCTGGTATCCGAGGATGAGCTCTCAATAGTGACTGAGCGAGCTTCTGATCTTGGAATCTTTAACCCTGGTAGTGGTGATATGGAGATCATCGCAGGCGAAGCTTTCAGAAGACTCAACTCCATGGAAAGACTAAATAAGGTGGATTTGATATCTGTGATGGGAAGTTCTCTCCCAAGAGACAAGCTTCTCATGGTGGAGCGCTTAAAGAAAAACGGTCACATAGTAGCGTTCTATGGAGGGTTAACCATAAATGATACAAGGACTCTGAAAGAAGCTGATGTTGGAATCACAGAGGATATATGGAGCACTGAGATGGCCAGAGACAATGCTGATATTATAGTTAAGGATGGCTGCTCATGGTTCCTGAACTTTAAGTCTGGGAAATGTGCTTACCACAACATTCAGAACTTCTGTCAAATTCAGCTCACGGCTTGCATATCCGGGTTGCTAGTAACGTTAGTAGCAACGATGCATTCAGGGGACTCTCCATTGACCGCAATTCACTTGATTTGGGTGAACTTGATAATGTGCCTTCTAGGCAGCCTAATGATGGTAATGGAGTTAGAACGCCAGGAGCCACTTGCACAAGAACCAGCGCCAAGGACTCAAGAACCGGCCCCAAGTACTCAAGAACCGGCACGGACTCAGCCACTTATAACCAAAGCCATTTGGGGAAACATAGCCAGTCAAGTTTCATACCAGGCTTCCATCTTGCTGGTCTTGCATTTCATGGGAAATGCAATAGCGAGCATGAACCAAGGCGTCCGGGGTACCATGGTTTTCAATATTTACACCTTGTGTCAGGTCCTTAATCTGTTCAGGGCTATGGACCTAGTGAACAAGGAAGTGCTAAATGTTGTTCTTCACAGCTATTGGTTTTTGATGGCCTTGGGGGCTGTTATGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGACTAGCCAGTGGTGTGAGGTTGGATGCAATACATTGGGCATCCTGCTTCCTTCTTGCTGCTCTTTCATGGCCGTTTGATTGGGTCATAACTTCGCAGTTTCGAAGAGAAAATTTGGCATGGAGGAGAGCACCTATGGGGATGTCAAGATTTTACATTTGTGCTTCGTTGTTCCTCATTTTCTCTGTATCATATTGTTTCGAATCAGATTATGCACACACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

866

Amino Acids

97.11

Weight (kDa)

6.01

Isoelectric Point (pI)

33.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	49 - 141	5.4e-09	P-type ATPase actuator domain
Cation_ATPase	PF13246	315 - 461	6.6e-11	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	641 - 820	2e-17	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1058, 1197, 2093
AarI	CACCTGC	1 cut(s)	2338
Acc36I	ACCTGC	2 cut(s)	1243, 2338
Acc65I	GGTACC	1 cut(s)	2207
AccB1I	GGYRCC	1 cut(s)	2207
AccBSI	CCGCTC	1 cut(s)	176
AciI	CCGC	2 cut(s)	176, 1932
AclI	AACGTT	1 cut(s)	1892
AclWI	GGATC	4 cut(s)	425, 438, 776, 1053
AcoI	YGGCCR	2 cut(s)	1752, 2444
AcsI	RAATTY	6 cut(s)	137, 639, 1230, 1325, 1854, 2485
AcuI	CTGAAG	2 cut(s)	63, 614
AcyI	GRCGYC	1 cut(s)	2199
AdeI	CACNNNGTG	1 cut(s)	2235
AfaI	GTAC	2 cut(s)	2062, 2209
AfeI	AGCGCT	1 cut(s)	1637
AfiI	CCNNNNNNNGG	7 cut(s)	83, 245, 598, 775, 871, 1508, 1987
AflIII	ACRYGT	1 cut(s)	1182
AhlI	ACTAGT	1 cut(s)	845
AjnI	CCWGG	4 cut(s)	357, 1501, 2007, 2136
AjuI	GAANNNNNNNTTGG	2 cut(s)	1701, 1733
Alw21I	GWGCWC	4 cut(s)	1135, 1458, 1745, 2506
Alw26I	GTCTC	4 cut(s)	222, 670, 1608, 1752
Alw44I	GTGCAC	1 cut(s)	1131
AlwI	GGATC	4 cut(s)	425, 438, 776, 1053
Aor51HI	AGCGCT	1 cut(s)	1637
AoxI	GGCC	7 cut(s)	355, 584, 1419, 1752, 2052, 2322, 2444
ApaLI	GTGCAC	1 cut(s)	1131
ApeKI	GCWGC	5 cut(s)	1144, 1147, 1788, 1977, 2432
ApoI	RAATTY	6 cut(s)	137, 639, 1230, 1325, 1854, 2485
Asp700I	GAANNNNTTC	1 cut(s)	629
Asp718I	GGTACC	1 cut(s)	2207
AspLEI	GCGC	2 cut(s)	1638, 2035
AspS9I	GGNCC	5 cut(s)	355, 766, 2053, 2242, 2266
AsuC2I	CCSGG	2 cut(s)	1879, 2204
AsuHPI	GGTGA	8 cut(s)	124, 215, 433, 799, 905, 1523, 1963, 2365
AsuII	TTCGAA	2 cut(s)	2476, 2574
AvaII	GGWCC	3 cut(s)	766, 2242, 2266
BaeGI	GKGCMC	1 cut(s)	1135
BaeI	ACNNNNGTAYC	2 cut(s)	2199, 2232
BalI	TGGCCA	1 cut(s)	1754
BamHI	GGATCC	1 cut(s)	430
BanI	GGYRCC	1 cut(s)	2207
BanII	GRGCYC	2 cut(s)	299, 1458
BbsI	GAAGAC	2 cut(s)	455, 1552
Bbv12I	GWGCWC	4 cut(s)	1135, 1458, 1745, 2506
BbvI	GCAGC	5 cut(s)	1134, 1156, 1775, 1989, 2419
BceAI	ACGGC	2 cut(s)	1881, 2431
BcgI	CGANNNNNNTGC	2 cut(s)	972, 1006
BciT130I	CCWGG	4 cut(s)	359, 1503, 2009, 2138
BciVI	GTATCC	2 cut(s)	699, 1453
BclI	TGATCA	1 cut(s)	925
BcnI	CCSGG	2 cut(s)	1879, 2204
BcoDI	GTCTC	4 cut(s)	222, 670, 1608, 1752
BcuI	ACTAGT	1 cut(s)	845
BfaI	CTAG	7 cut(s)	171, 551, 846, 1886, 1973, 2270, 2378
BfoI	RGCGCY	2 cut(s)	1639, 2036
BfuAI	ACCTGC	2 cut(s)	1243, 2338
BfuI	GTATCC	2 cut(s)	699, 1453
BglI	GCCNNNNNGGC	2 cut(s)	1974, 2330
BisI	GCNGC	5 cut(s)	1145, 1148, 1789, 1978, 2433
BlpI	GCTNAGC	1 cut(s)	413
BlsI	GCNGC	5 cut(s)	1146, 1149, 1790, 1979, 2434
BmcAI	AGTACT	1 cut(s)	2062
Bme1390I	CCNGG	6 cut(s)	359, 1503, 1879, 2009, 2138, 2204
Bme18I	GGWCC	3 cut(s)	766, 2242, 2266
BmgT120I	GGNCC	5 cut(s)	355, 766, 2053, 2242, 2266
BmiI	GGNNCC	5 cut(s)	432, 1799, 2013, 2055, 2209
BmrFI	CCNGG	6 cut(s)	359, 1503, 1879, 2009, 2138, 2204
BmsI	GCATC	7 cut(s)	1113, 1153, 1175, 1361, 1896, 2389, 2423
BpiI	GAAGAC	2 cut(s)	455, 1552
Bpu1102I	GCTNAGC	1 cut(s)	413
Bpu14I	TTCGAA	2 cut(s)	2476, 2574
BpuEI	CTTGAG	3 cut(s)	639, 2028, 2049
BpuMI	CCSGG	2 cut(s)	1879, 2204
BsaBI	GATNNNNATC	1 cut(s)	435
BsaHI	GRCGYC	1 cut(s)	2199
BsaI	GGTCTC	2 cut(s)	222, 670
Bsc4I	CCNNNNNNNGG	7 cut(s)	83, 245, 598, 775, 871, 1508, 1987
Bse118I	RCCGGY	2 cut(s)	2050, 2071
Bse1I	ACTGG	4 cut(s)	279, 1362, 2121, 2382
Bse3DI	GCAATG	1 cut(s)	916
Bse8I	GATNNNNATC	1 cut(s)	435
BseBI	CCWGG	4 cut(s)	359, 1503, 2009, 2138
BseGI	GGATG	9 cut(s)	154, 289, 894, 1104, 1456, 1789, 2404, 2414, 2520
BseJI	GATNNNNATC	1 cut(s)	435
BseLI	CCNNNNNNNGG	7 cut(s)	83, 245, 598, 775, 871, 1508, 1987
BseMI	GCAATG	1 cut(s)	916
BseMII	CTCAG	5 cut(s)	404, 417, 1461, 1737, 2096
BseNI	ACTGG	4 cut(s)	279, 1362, 2121, 2382
BseRI	GAGGAG	2 cut(s)	1286, 2512
BseSI	GKGCMC	1 cut(s)	1135
BseXI	GCAGC	5 cut(s)	1134, 1156, 1775, 1989, 2419
BseYI	CCCAGC	1 cut(s)	979
BshFI	GGCC	7 cut(s)	357, 586, 1421, 1754, 2054, 2324, 2446
BshNI	GGYRCC	1 cut(s)	2207
BsiHKAI	GWGCWC	4 cut(s)	1135, 1458, 1745, 2506
BsiSI	CCGG	5 cut(s)	1422, 1878, 2051, 2072, 2203
BslFI	GGGAC	2 cut(s)	828, 1931
BslI	CCNNNNNNNGG	7 cut(s)	83, 245, 598, 775, 871, 1508, 1987
BsmAI	GTCTC	4 cut(s)	222, 670, 1608, 1752
BsmFI	GGGAC	2 cut(s)	828, 1931
BsmI	GAATGC	2 cut(s)	723, 1909
BsnI	GGCC	7 cut(s)	357, 586, 1421, 1754, 2054, 2324, 2446
Bso31I	GGTCTC	2 cut(s)	222, 670
Bsp119I	TTCGAA	2 cut(s)	2476, 2574
Bsp1286I	GDGCHC	5 cut(s)	299, 1135, 1458, 1745, 2506
Bsp143I	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
Bsp1720I	GCTNAGC	1 cut(s)	413
Bsp19I	CCATGG	3 cut(s)	810, 1557, 2211
BspACI	CCGC	2 cut(s)	176, 1932
BspANI	GGCC	7 cut(s)	357, 586, 1421, 1754, 2054, 2324, 2446
BspCNI	CTCAG	5 cut(s)	405, 418, 1462, 1738, 2095
BspLI	GGNNCC	5 cut(s)	432, 1799, 2013, 2055, 2209
BspMI	ACCTGC	2 cut(s)	1243, 2338
BspPI	GGATC	4 cut(s)	425, 438, 776, 1053
BspQI	GCTCTTC	1 cut(s)	1380
BspT104I	TTCGAA	2 cut(s)	2476, 2574
BspT107I	GGYRCC	1 cut(s)	2207
BspTNI	GGTCTC	2 cut(s)	222, 670
BsrBI	CCGCTC	1 cut(s)	176
BsrDI	GCAATG	1 cut(s)	916
BsrFI	RCCGGY	2 cut(s)	2050, 2071
BsrI	ACTGG	4 cut(s)	279, 1362, 2121, 2382
BssAI	RCCGGY	2 cut(s)	2050, 2071
BssMI	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
BssNI	GRCGYC	1 cut(s)	2199
BssT1I	CCWWGG	6 cut(s)	810, 1557, 2036, 2194, 2211, 2325
Bst2UI	CCWGG	4 cut(s)	359, 1503, 2009, 2138
Bst4CI	ACNGT	6 cut(s)	35, 55, 546, 694, 826, 1652
Bst6I	CTCTTC	4 cut(s)	1062, 1232, 1380, 2473
BstACI	GRCGYC	1 cut(s)	2199
BstBI	TTCGAA	2 cut(s)	2476, 2574
BstC8I	GCNNGC	4 cut(s)	1476, 1531, 1870, 2185
BstDEI	CTNAG	7 cut(s)	413, 426, 1177, 1283, 1470, 1746, 2082
BstDSI	CCRYGG	3 cut(s)	810, 1557, 2211
BstF5I	GGATG	9 cut(s)	154, 289, 894, 1104, 1456, 1789, 2404, 2414, 2520
BstH2I	RGCGCY	2 cut(s)	1639, 2036
BstHHI	GCGC	2 cut(s)	1638, 2035
BstKTI	GATC	8 cut(s)	262, 433, 784, 928, 1008, 1048, 1486, 1524
BstMAI	GTCTC	4 cut(s)	222, 670, 1608, 1752
BstMBI	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
BstMWI	GCNNNNNNNGC	6 cut(s)	980, 1406, 1535, 1974, 2158, 2330
BstNI	CCWGG	4 cut(s)	359, 1503, 2009, 2138
BstNSI	RCATGY	2 cut(s)	507, 1186
BstSCI	CCNGG	6 cut(s)	357, 1501, 1877, 2007, 2136, 2202
BstSLI	GKGCMC	1 cut(s)	1135
BstV1I	GCAGC	5 cut(s)	1134, 1156, 1775, 1989, 2419
BstV2I	GAAGAC	2 cut(s)	455, 1552
BstX2I	RGATCY	2 cut(s)	430, 781
BstXI	CCANNNNNNTGG	1 cut(s)	2152
BstYI	RGATCY	2 cut(s)	430, 781
BsuI	GTATCC	2 cut(s)	699, 1453
BsuRI	GGCC	7 cut(s)	357, 586, 1421, 1754, 2054, 2324, 2446
BtgI	CCRYGG	3 cut(s)	810, 1557, 2211
BtgZI	GCGATG	1 cut(s)	1510
BtsCI	GGATG	9 cut(s)	154, 289, 894, 1104, 1456, 1789, 2404, 2414, 2520
BtsI	GCAGTG	2 cut(s)	1135, 1407
BtsIMutI	CAGTG	5 cut(s)	1135, 1355, 1407, 1743, 2389
BveI	ACCTGC	2 cut(s)	1243, 2338
Cac8I	GCNNGC	4 cut(s)	1476, 1531, 1870, 2185
CfoI	GCGC	2 cut(s)	1638, 2035
Cfr10I	RCCGGY	2 cut(s)	2050, 2071
Cfr13I	GGNCC	5 cut(s)	355, 766, 2053, 2242, 2266
CseI	GACGC	1 cut(s)	2188
Csp6I	GTAC	2 cut(s)	2061, 2208
CviQI	GTAC	2 cut(s)	2061, 2208
DdeI	CTNAG	7 cut(s)	413, 426, 1177, 1283, 1470, 1746, 2082
DpnI	GATC	8 cut(s)	261, 432, 783, 927, 1007, 1047, 1485, 1523
DpnII	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
DraIII	CACNNNGTG	1 cut(s)	2235
EaeI	YGGCCR	2 cut(s)	1752, 2444
Eam1104I	CTCTTC	4 cut(s)	1062, 1232, 1380, 2473
EarI	CTCTTC	4 cut(s)	1062, 1232, 1380, 2473
Ecl136II	GAGCTC	1 cut(s)	1456
Eco130I	CCWWGG	6 cut(s)	810, 1557, 2036, 2194, 2211, 2325
Eco24I	GRGCYC	2 cut(s)	299, 1458
Eco31I	GGTCTC	2 cut(s)	222, 670
Eco32I	GATATC	2 cut(s)	186, 1587
Eco47I	GGWCC	3 cut(s)	766, 2242, 2266
Eco47III	AGCGCT	1 cut(s)	1637
Eco53kI	GAGCTC	1 cut(s)	1456
Eco57I	CTGAAG	2 cut(s)	63, 614
EcoICRI	GAGCTC	1 cut(s)	1456
EcoO109I	RGGNCCY	1 cut(s)	2242
EcoRII	CCWGG	4 cut(s)	357, 1501, 2007, 2136
EcoRV	GATATC	2 cut(s)	186, 1587
EcoT14I	CCWWGG	6 cut(s)	810, 1557, 2036, 2194, 2211, 2325
EcoT22I	ATGCAT	2 cut(s)	1168, 1911
EcoT38I	GRGCYC	2 cut(s)	299, 1458
ErhI	CCWWGG	6 cut(s)	810, 1557, 2036, 2194, 2211, 2325
FalI	AAGNNNNNCTT	2 cut(s)	1374, 1406
FaqI	GGGAC	2 cut(s)	828, 1931
FauNDI	CATATG	2 cut(s)	1152, 1297
FbaI	TGATCA	1 cut(s)	925
Fnu4HI	GCNGC	5 cut(s)	1145, 1148, 1789, 1978, 2433
FokI	GGATG	9 cut(s)	161, 296, 901, 1091, 1463, 1796, 2401, 2411, 2527
FriOI	GRGCYC	2 cut(s)	299, 1458
Fsp4HI	GCNGC	5 cut(s)	1145, 1148, 1789, 1978, 2433
FspBI	CTAG	7 cut(s)	171, 551, 846, 1886, 1973, 2270, 2378
GlaI	GCGC	2 cut(s)	1637, 2034
GluI	GCNGC	5 cut(s)	1145, 1148, 1789, 1978, 2433
GsaI	CCCAGC	1 cut(s)	983
HaeII	RGCGCY	2 cut(s)	1639, 2036
HaeIII	GGCC	7 cut(s)	357, 586, 1421, 1754, 2054, 2324, 2446
HapII	CCGG	5 cut(s)	1422, 1878, 2051, 2072, 2203
HgaI	GACGC	1 cut(s)	2188
HhaI	GCGC	2 cut(s)	1638, 2035
Hin1I	GRCGYC	1 cut(s)	2199
Hin6I	GCGC	2 cut(s)	1636, 2033
HinP1I	GCGC	2 cut(s)	1636, 2033
HincII	GTYRAC	1 cut(s)	1680
HindII	GTYRAC	1 cut(s)	1680
HindIII	AAGCTT	4 cut(s)	462, 1407, 1536, 1619
HpaI	GTTAAC	1 cut(s)	1680
HpaII	CCGG	5 cut(s)	1422, 1878, 2051, 2072, 2203
HphI	GGTGA	8 cut(s)	124, 215, 433, 799, 905, 1523, 1963, 2365
Hpy166II	GTNNAC	5 cut(s)	1133, 1190, 1680, 1954, 2275
Hpy8I	GTNNAC	5 cut(s)	1133, 1190, 1680, 1954, 2275
HpyAV	CCTTC	9 cut(s)	87, 217, 589, 654, 748, 754, 1270, 1979, 2435
HpyCH4III	ACNGT	6 cut(s)	35, 55, 546, 694, 826, 1652
HpyCH4IV	ACGT	2 cut(s)	958, 1892
HpyF10VI	GCNNNNNNNGC	6 cut(s)	980, 1406, 1535, 1974, 2158, 2330
HpyF3I	CTNAG	7 cut(s)	413, 426, 1177, 1283, 1470, 1746, 2082
HpySE526I	ACGT	2 cut(s)	958, 1892
Hsp92I	GRCGYC	1 cut(s)	2199
HspAI	GCGC	2 cut(s)	1636, 2033
KpnI	GGTACC	1 cut(s)	2211
Ksp22I	TGATCA	1 cut(s)	925
KspAI	GTTAAC	1 cut(s)	1680
Kzo9I	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
LguI	GCTCTTC	1 cut(s)	1380
LmnI	GCTCC	4 cut(s)	241, 1633, 1740, 2011
Lsp1109I	GCAGC	5 cut(s)	1134, 1156, 1775, 1989, 2419
LweI	GCATC	7 cut(s)	1113, 1153, 1175, 1361, 1896, 2389, 2423
MaeI	CTAG	7 cut(s)	171, 551, 846, 1886, 1973, 2270, 2378
MaeII	ACGT	2 cut(s)	958, 1892
MalI	GATC	8 cut(s)	261, 432, 783, 927, 1007, 1047, 1485, 1523
MbiI	CCGCTC	1 cut(s)	176
MboI	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
MfeI	CAATTG	1 cut(s)	303
MflI	RGATCY	2 cut(s)	430, 781
MhlI	GDGCHC	5 cut(s)	299, 1135, 1458, 1745, 2506
MlsI	TGGCCA	1 cut(s)	1754
MluNI	TGGCCA	1 cut(s)	1754
MlyI	GAGTC	9 cut(s)	21, 445, 810, 929, 1542, 1693, 1913, 2035, 2074
MmeI	TCCRAC	2 cut(s)	1699, 2376
Mox20I	TGGCCA	1 cut(s)	1754
Mph1103I	ATGCAT	2 cut(s)	1168, 1911
MroXI	GAANNNNTTC	1 cut(s)	629
MscI	TGGCCA	1 cut(s)	1754
MslI	CAYNNNNRTG	6 cut(s)	33, 1109, 1155, 1197, 1748, 2348
Msp20I	TGGCCA	1 cut(s)	1754
MspA1I	CMGCKG	1 cut(s)	1147
MspI	CCGG	5 cut(s)	1422, 1878, 2051, 2072, 2203
MspR9I	CCNGG	6 cut(s)	359, 1503, 1879, 2009, 2138, 2204
MunI	CAATTG	1 cut(s)	303
Mva1269I	GAATGC	2 cut(s)	723, 1909
MvaI	CCWGG	4 cut(s)	359, 1503, 2009, 2138
MwoI	GCNNNNNNNGC	6 cut(s)	980, 1406, 1535, 1974, 2158, 2330
NciI	CCSGG	2 cut(s)	1879, 2204
NcoI	CCATGG	3 cut(s)	810, 1557, 2211
NdeI	CATATG	2 cut(s)	1152, 1297
NdeII	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
NlaIV	GGNNCC	5 cut(s)	432, 1799, 2013, 2055, 2209
NmuCI	GTSAC	5 cut(s)	373, 421, 805, 1465, 1652
NsiI	ATGCAT	2 cut(s)	1168, 1911
NspI	RCATGY	2 cut(s)	507, 1186
NspV	TTCGAA	2 cut(s)	2476, 2574
PaqCI	CACCTGC	1 cut(s)	2338
PciI	ACATGT	1 cut(s)	1182
PciSI	GCTCTTC	1 cut(s)	1380
PctI	GAATGC	2 cut(s)	723, 1909
PdmI	GAANNNNTTC	1 cut(s)	629
PfeI	GAWTC	7 cut(s)	167, 790, 989, 1431, 1491, 1722, 2576
PflFI	GACNNNGTC	1 cut(s)	957
PkrI	GCNGC	5 cut(s)	1146, 1149, 1790, 1979, 2434
PleI	GAGTC	9 cut(s)	20, 445, 810, 928, 1542, 1693, 1913, 2035, 2074
PpsI	GAGTC	9 cut(s)	20, 445, 810, 928, 1542, 1693, 1913, 2035, 2074
PpuMI	RGGWCCY	1 cut(s)	2242
PscI	ACATGT	1 cut(s)	1182
PsiI	TTATAA	3 cut(s)	1058, 1197, 2093
Psp124BI	GAGCTC	1 cut(s)	1458
Psp1406I	AACGTT	1 cut(s)	1892
Psp5II	RGGWCCY	1 cut(s)	2242
Psp6I	CCWGG	4 cut(s)	357, 1501, 2007, 2136
PspFI	CCCAGC	1 cut(s)	979
PspGI	CCWGG	4 cut(s)	357, 1501, 2007, 2136
PspN4I	GGNNCC	5 cut(s)	432, 1799, 2013, 2055, 2209
PspPI	GGNCC	5 cut(s)	355, 766, 2053, 2242, 2266
PspPPI	RGGWCCY	1 cut(s)	2242
PsuI	RGATCY	2 cut(s)	430, 781
PsyI	GACNNNGTC	1 cut(s)	957
PvuII	CAGCTG	1 cut(s)	1147
RsaI	GTAC	2 cut(s)	2062, 2209
RsaNI	GTAC	2 cut(s)	2061, 2208
RseI	CAYNNNNRTG	6 cut(s)	33, 1109, 1155, 1197, 1748, 2348
SacI	GAGCTC	1 cut(s)	1458
SapI	GCTCTTC	1 cut(s)	1380
SatI	GCNGC	5 cut(s)	1145, 1148, 1789, 1978, 2433
Sau3AI	GATC	8 cut(s)	259, 430, 781, 925, 1005, 1045, 1483, 1521
Sau96I	GGNCC	5 cut(s)	355, 766, 2053, 2242, 2266
ScaI	AGTACT	1 cut(s)	2062
SchI	GAGTC	9 cut(s)	21, 445, 810, 929, 1542, 1693, 1913, 2035, 2074
ScrFI	CCNGG	6 cut(s)	359, 1503, 1879, 2009, 2138, 2204
SduI	GDGCHC	5 cut(s)	299, 1135, 1458, 1745, 2506
SfaNI	GCATC	7 cut(s)	1113, 1153, 1175, 1361, 1896, 2389, 2423
SfuI	TTCGAA	2 cut(s)	2476, 2574
SinI	GGWCC	3 cut(s)	766, 2242, 2266
SmiMI	CAYNNNNRTG	6 cut(s)	33, 1109, 1155, 1197, 1748, 2348
SmlI	CTYRAG	3 cut(s)	654, 2043, 2064
SmoI	CTYRAG	3 cut(s)	654, 2043, 2064
SpeI	ACTAGT	1 cut(s)	845
SsiI	CCGC	2 cut(s)	176, 1932
SspI	AATATT	1 cut(s)	2224
SspMI	CTAG	7 cut(s)	171, 551, 846, 1886, 1973, 2270, 2378
SstI	GAGCTC	1 cut(s)	1458
StyD4I	CCNGG	6 cut(s)	357, 1501, 1877, 2007, 2136, 2202
StyI	CCWWGG	6 cut(s)	810, 1557, 2036, 2194, 2211, 2325
TaaI	ACNGT	6 cut(s)	35, 55, 546, 694, 826, 1652
TaiI	ACGT	2 cut(s)	961, 1895
TaqI	TCGA	6 cut(s)	364, 390, 633, 1004, 2476, 2574
TaqII	GACCGA	1 cut(s)	1303
TatI	WGTACW	1 cut(s)	2060
TfiI	GAWTC	7 cut(s)	167, 790, 989, 1431, 1491, 1722, 2576
TscAI	CASTG	5 cut(s)	1135, 1362, 1407, 1750, 2389
TseFI	GTSAC	5 cut(s)	373, 421, 805, 1465, 1652
TseI	GCWGC	5 cut(s)	1144, 1147, 1788, 1977, 2432
Tsp45I	GTSAC	5 cut(s)	373, 421, 805, 1465, 1652
TspGWI	ACGGA	1 cut(s)	2092
TspRI	CASTG	5 cut(s)	1135, 1362, 1407, 1750, 2389
Tth111I	GACNNNGTC	1 cut(s)	957
VneI	GTGCAC	1 cut(s)	1131
VpaK11BI	GGWCC	3 cut(s)	766, 2242, 2266
XapI	RAATTY	6 cut(s)	137, 639, 1230, 1325, 1854, 2485
XbaI	TCTAGA	1 cut(s)	170
XceI	RCATGY	2 cut(s)	507, 1186
XmnI	GAANNNNTTC	1 cut(s)	629
XspI	CTAG	7 cut(s)	171, 551, 846, 1886, 1973, 2270, 2378
ZrmI	AGTACT	1 cut(s)	2062
Zsp2I	ATGCAT	2 cut(s)	1168, 1911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.