Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Reverse (-)

3472269 .. 3475196

Gene Structure 2928 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g04710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2826 bp

ATGGCCCTCGAGATAAATACGGAGGATCAAGCTGCTGAAGGTGCCTCTCTTACTGCCGGCTTGATTATGCAGACCATCGGACGCAGGGGAACCTCGCTGAGATATGTTGTTCTAGGCATTTTGTTCTCATCACGCATTAAGAAAACCAGACGCCTGCGTGAAGCTAGTTATGAACCTTTAGCAGCAGCACCAGCGCCACCAGCCACTGACCATGTGATTGACATCCCTCTCCAGGAACGGTTTTTGAACAAGGTTGCTCGAATTGTGAGAGAGAAGGACTTGAACTCACTCCACGGACATGGTGGTGTTGAGCGCATCTTGCCACTTCTCAGGTCTCACTTTGAGGAAGGTGCCGTTGATGGTGGTCAAAATCCAGAAGGATGGAACATTACCAAGTCCCCAGTTGAAGCAAAAGGTTTCTTGTACTTTTTGTTTGAGGCGTGCAATCGGTACACGATTTTTTTCCTCTTTCTCTCAGCTGGGTTTTCATTCGCCATCGAATTCATGAAGCAAGGAGTCAAGGATGGCTGGCATGACGGTGTTGCCATACTCATCACCGTGTTCTTACTTATTGCTTTCCCTACGGTGCAGAACTACCGCAATCAGAGAAAGTCGGTGCAGAAGCACTTGCTGGATAGGAGCAAACTGATGGTGAATGTTGAAAGAAGTAGCCCAGAACCTACACCGATAACCATATCTGGCGTTGTGGTGGGTGATATAGTTCATTTGAAGGAAGGAGACCGTGTTCTTGTTGACGGTTTGTTCATAGATCAGGGTGAGGAACTGGTGGTGGATGAGGTATTGAGCCCGAAAATTGATTGTGAGCAAAATCCATTTGTTTTTTCGGGTTCAAAGGTTGTTAAGGGCCGCGGCCGCATGATTGTGACATCAGTTGGTGCTAATACAGCTATTGCCGAGATGCTGAGCTTGGTGTCTGATCGTAATCCATTGGAAAAGACACTGCTACAAGCTTTGATGGACAAACCGAATGCTTGTATGGATTTTCTTGCATGTTGTGTCTCGATACTGATTGCGCTTGTGATGTTGATACGCCTTCTATTCTTCAAGAAGTACGAAAACTACAACGAGAGGCCAGACCTGAAAGGTGAAGTTTCAATGAATTTTGTGATGAGGATCTTTGAGAATATCTTCTTGAAACCTCAAGGAAAGGTTTCCATCTTAGCAAGCGTTCTTGCAACTGTGGTAATAGGGATACAGCATGGGATGCAAGTTGTAATTACAGTTGCCCTTTTTCAATGGAAGGAGAAGGCGGCCGAAAATCAGGCAGATCCACAGGATCTATCAGCTTCTGTCACCATGGGACTCATAACAGTCATCTGCATTGAAACAACGGCAGATCTAATGTGCAACCAGACGGAGGTCAAGGAATTTTGGATCGGCGAAAAGGATTTAAGCAGTGGTGAAGTGGACTCAGAAACTGACCAAGTTCTTGAGGCTCTCCATCAGGGGATTTCAGCTACCGCATCACCAGCAAATGATCGTCTCATTTCTTGGCTGAACACCAGATGGGGTAGTAACGCCGCGTTAGTTGATCGAAGATTTGCAACTGTTGAGCAGAGACAATGGAGCTCTAATGAGAAATATAGAGGCGTCTTAGTGAGGAAAATCGAGAATGATGAGCAGATTATGCAACTTCACTGCGACGGAGAAGCATCAACAATATTAGGCATGTGTTCACATTACTATGATAACAGAGGGGAAAGTCAAAATATTGGAAACCAGAAGAGAAAATTTGAAGAGGTGATCAAGAAAATGGAGAAGAAGGGTCTTACACCGATTGCATACGCGTACAAGAAAACAGAAGCCGAAGATCCTACAGAAGATGGGTTGATTTTGTTGGCACTTGTCGGTCTTAGATGTCCATATCAAGAAGAGCTTAAATTGGCAGTGGAGGCTCTCAGAAAAGCTGGAGTAATCATCAAACTGGTGTCGGAGGATGAGCTCTCGAAAGTGAGAGCTAGAGCTTCGGAGCTTGGGATCTCCCCTGGCTCAGATGACACGGAGATTGAAGGTCAAGCTTTCCGAAGGCTCAATTCCATGGAAAAACTGGATAAGGTGGATTTGATCTCTGTGATGGGAAGTTCCCTCCCCAAGGACAAGTTTCTCATGGTGGAGAGGTTAAAGAAAAAAGGTCACATAGTTGCATTCTATGGAGGGCTAACCATCAGTGACACTTTGACCTTAAAAGAAGCTGATATAGGAATTGTTGAGGATCTCTGGAGCACCGAGATGGCCAGAGAGAACGCCGATCTTATAATCAGAAACGGATGTTTCTTGAGCATGAATATGAGGTCTGGTGCTTGTGCTTACCACAACATTCAGCAGTTCACTCAACTTCAGCTCACCGCTTGCATATCGGGGTTACTAGTAACCCTAGTCGCAACGATGCATTTGGGAGAGTCCCCGCTAACAGCAGTTCACTTGATTTGGGTGAACTTGATAATGTGTCTTCTTGGTGGCCCAATGATGGCGATGGAGTTACAAGGCACCGAACGACACACTCAAAGACCTGCGAAGAGGACTGAGTCGCTTATAACCAAAGTCATCTGGAGAAACATAGCAGTTCAAGTTTCGTATCAGGCTTCCGTCTTGCTGATCTTACATTGCATGGGAAACATAGTACCGAGCATGAACGAAGGCGTCCGGAATACCATGATTTTCAATACTTTCACCTTATGCCAGGTCCTTAATATGTTCAGTACCATGGACATAGTAAAAAAGGAAGTGCTACTAGTTGTCCTCCGAAGCCATTGGTTTCTGATGGCGTTGTCGGCTGTTTTGATCGATCCATGTGATCATTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

942

Amino Acids

104.89

Weight (kDa)

6.03

Isoelectric Point (pI)

39.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	228 - 311	1.1e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	493 - 631	2e-09	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	808 - 933	3.1e-18	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2276, 2555
Acc36I	ACCTGC	1 cut(s)	2539
AccB1I	GGYRCC	3 cut(s)	41, 350, 2507
AccII	CGCG	3 cut(s)	870, 1544, 1808
AccIII	TCCGGA	1 cut(s)	2664
AciI	CCGC	9 cut(s)	598, 868, 870, 874, 1271, 1482, 1542, 2367, 2426
AclWI	GGATC	9 cut(s)	33, 1142, 1283, 1305, 1403, 1826, 2006, 2241, 2801
AcoI	YGGCCR	3 cut(s)	871, 1272, 2253
AcsI	RAATTY	4 cut(s)	500, 1120, 1388, 1751
AcuI	CTGAAG	2 cut(s)	57, 2342
AcyI	GRCGYC	3 cut(s)	151, 1611, 2661
AfaI	GTAC	6 cut(s)	425, 452, 1073, 1811, 2643, 2722
AfiI	CCNNNNNNNGG	4 cut(s)	232, 1378, 2113, 2488
AflIII	ACRYGT	1 cut(s)	1806
AhlI	ACTAGT	2 cut(s)	2386, 2752
AjnI	CCWGG	3 cut(s)	231, 2005, 2700
AloI	GAACNNNNNNTCC	2 cut(s)	729, 761
Alw21I	GWGCWC	3 cut(s)	1592, 1965, 2246
Alw26I	GTCTC	5 cut(s)	339, 732, 1024, 1508, 1573
AlwI	GGATC	9 cut(s)	33, 1142, 1283, 1305, 1403, 1826, 2006, 2241, 2801
AlwNI	CAGNNNCTG	3 cut(s)	206, 1310, 1439
Ama87I	CYCGRG	1 cut(s)	8
Aor13HI	TCCGGA	1 cut(s)	2664
AoxI	GGCC	7 cut(s)	3, 865, 871, 1091, 1272, 2253, 2479
ApeKI	GCWGC	3 cut(s)	32, 182, 185
ApoI	RAATTY	4 cut(s)	500, 1120, 1388, 1751
Asp700I	GAANNNNTTC	2 cut(s)	1148, 1171
AspLEI	GCGC	3 cut(s)	196, 315, 1036
AspS9I	GGNCC	4 cut(s)	4, 865, 2480, 2704
AvaI	CYCGRG	1 cut(s)	8
AvaII	GGWCC	1 cut(s)	2704
BalI	TGGCCA	1 cut(s)	2255
BanI	GGYRCC	3 cut(s)	41, 350, 2507
BanII	GRGCYC	3 cut(s)	809, 1592, 1965
BbsI	GAAGAC	1 cut(s)	2462
Bbv12I	GWGCWC	3 cut(s)	1592, 1965, 2246
BbvI	GCAGC	3 cut(s)	19, 194, 197
BceAI	ACGGC	2 cut(s)	338, 1368
BciT130I	CCWGG	3 cut(s)	233, 2007, 2702
BciVI	GTATCC	1 cut(s)	1206
BclI	TGATCA	2 cut(s)	1764, 2815
BcoDI	GTCTC	5 cut(s)	339, 732, 1024, 1508, 1573
BcuI	ACTAGT	2 cut(s)	2386, 2752
BfaI	CTAG	6 cut(s)	113, 165, 1980, 2387, 2396, 2753
BfmI	CTRYAG	1 cut(s)	1836
BfoI	RGCGCY	1 cut(s)	197
BfuAI	ACCTGC	1 cut(s)	2539
BfuI	GTATCC	1 cut(s)	1206
BglII	AGATCT	1 cut(s)	1357
BisI	GCNGC	8 cut(s)	33, 183, 186, 868, 871, 874, 1272, 1542
BlpI	GCTNAGC	1 cut(s)	923
BlsI	GCNGC	8 cut(s)	34, 184, 187, 869, 872, 875, 1273, 1543
Bme1390I	CCNGG	3 cut(s)	233, 2007, 2702
Bme18I	GGWCC	1 cut(s)	2704
BmeT110I	CYCGRG	1 cut(s)	8
BmgT120I	GGNCC	4 cut(s)	4, 865, 2480, 2704
BmiI	GGNNCC	4 cut(s)	43, 91, 352, 2509
BmrFI	CCNGG	3 cut(s)	233, 2007, 2702
BmrI	ACTGGG	1 cut(s)	395
BmsI	GCATC	6 cut(s)	324, 909, 1215, 1493, 1682, 2397
BmuI	ACTGGG	1 cut(s)	395
BoxI	GACNNNNGTC	1 cut(s)	1379
BpiI	GAAGAC	1 cut(s)	2462
BpmI	CTGGAG	4 cut(s)	215, 1950, 2260, 2590
Bpu1102I	GCTNAGC	1 cut(s)	923
BpuEI	CTTGAG	3 cut(s)	1146, 1472, 2317
Bsa29I	ATCGAT	1 cut(s)	2805
BsaBI	GATNNNNATC	3 cut(s)	221, 942, 1133
BsaHI	GRCGYC	3 cut(s)	151, 1611, 2661
BsaI	GGTCTC	2 cut(s)	339, 732
BsaJI	CCNNGG	7 cut(s)	292, 868, 1317, 2005, 2058, 2112, 2724
BsaWI	WCCGGW	1 cut(s)	2664
BsaXI	ACNNNNNCTCC	2 cut(s)	729, 759
Bsc4I	CCNNNNNNNGG	4 cut(s)	232, 1378, 2113, 2488
Bse118I	RCCGGY	1 cut(s)	56
Bse1I	ACTGG	4 cut(s)	401, 789, 1950, 2073
Bse3DI	GCAATG	1 cut(s)	2623
Bse8I	GATNNNNATC	3 cut(s)	221, 942, 1133
BseAI	TCCGGA	1 cut(s)	2664
BseBI	CCWGG	3 cut(s)	233, 2007, 2702
BseCI	ATCGAT	1 cut(s)	2805
BseDI	CCNNGG	7 cut(s)	292, 868, 1317, 2005, 2058, 2112, 2724
BseGI	GGATG	7 cut(s)	222, 386, 529, 799, 1230, 1963, 2294
BseJI	GATNNNNATC	3 cut(s)	221, 942, 1133
BseLI	CCNNNNNNNGG	4 cut(s)	232, 1378, 2113, 2488
BseMI	GCAATG	1 cut(s)	2623
BseMII	CTCAG	8 cut(s)	89, 343, 489, 914, 1446, 1933, 2025, 2535
BseNI	ACTGG	4 cut(s)	401, 789, 1950, 2073
BseX3I	CGGCCG	2 cut(s)	871, 1272
BseXI	GCAGC	3 cut(s)	19, 194, 197
BseYI	CCCAGC	1 cut(s)	479
BsgI	GTGCAG	2 cut(s)	608, 638
Bsh1236I	CGCG	3 cut(s)	870, 1544, 1808
Bsh1285I	CGRYCG	2 cut(s)	874, 1275
BshFI	GGCC	7 cut(s)	5, 867, 873, 1093, 1274, 2255, 2481
BshNI	GGYRCC	3 cut(s)	41, 350, 2507
BshVI	ATCGAT	1 cut(s)	2805
BsiEI	CGRYCG	2 cut(s)	874, 1275
BsiHKAI	GWGCWC	3 cut(s)	1592, 1965, 2246
BsiHKCI	CYCGRG	1 cut(s)	8
BsiSI	CCGG	2 cut(s)	57, 2665
BslFI	GGGAC	3 cut(s)	382, 1335, 2407
BslI	CCNNNNNNNGG	4 cut(s)	232, 1378, 2113, 2488
BsmAI	GTCTC	5 cut(s)	339, 732, 1024, 1508, 1573
BsmBI	CGTCTC	1 cut(s)	1508
BsmFI	GGGAC	3 cut(s)	382, 1335, 2407
BsmI	GAATGC	2 cut(s)	994, 2165
BsnI	GGCC	7 cut(s)	5, 867, 873, 1093, 1274, 2255, 2481
Bso31I	GGTCTC	2 cut(s)	339, 732
BsoBI	CYCGRG	1 cut(s)	8
Bsp1286I	GDGCHC	4 cut(s)	809, 1592, 1965, 2246
Bsp13I	TCCGGA	1 cut(s)	2664
Bsp1720I	GCTNAGC	1 cut(s)	923
Bsp19I	CCATGG	3 cut(s)	1317, 2058, 2724
BspACI	CCGC	9 cut(s)	598, 868, 870, 874, 1271, 1482, 1542, 2367, 2426
BspANI	GGCC	7 cut(s)	5, 867, 873, 1093, 1274, 2255, 2481
BspCNI	CTCAG	8 cut(s)	90, 342, 488, 915, 1445, 1932, 2024, 2536
BspDI	ATCGAT	1 cut(s)	2805
BspEI	TCCGGA	1 cut(s)	2664
BspFNI	CGCG	3 cut(s)	870, 1544, 1808
BspHI	TCATGA	1 cut(s)	504
BspLI	GGNNCC	4 cut(s)	43, 91, 352, 2509
BspMI	ACCTGC	1 cut(s)	2539
BspPI	GGATC	9 cut(s)	33, 1142, 1283, 1305, 1403, 1826, 2006, 2241, 2801
BspQI	GCTCTTC	1 cut(s)	1887
BspT107I	GGYRCC	3 cut(s)	41, 350, 2507
BspTNI	GGTCTC	2 cut(s)	339, 732
BsrDI	GCAATG	1 cut(s)	2623
BsrFI	RCCGGY	1 cut(s)	56
BsrI	ACTGG	4 cut(s)	401, 789, 1950, 2073
BssAI	RCCGGY	1 cut(s)	56
BssECI	CCNNGG	7 cut(s)	292, 868, 1317, 2005, 2058, 2112, 2724
BssNI	GRCGYC	3 cut(s)	151, 1611, 2661
BssT1I	CCWWGG	4 cut(s)	1317, 2058, 2112, 2724
Bst2UI	CCWGG	3 cut(s)	233, 2007, 2702
Bst6I	CTCTTC	4 cut(s)	1739, 1752, 1887, 2531
BstACI	GRCGYC	3 cut(s)	151, 1611, 2661
BstAPI	GCANNNNNTGC	2 cut(s)	1225, 1648
BstC8I	GCNNGC	6 cut(s)	58, 155, 442, 530, 1186, 2371
BstDSI	CCRYGG	5 cut(s)	292, 868, 1317, 2058, 2724
BstENI	CCTNNNNNAGG	1 cut(s)	2111
BstF5I	GGATG	7 cut(s)	222, 386, 529, 799, 1230, 1963, 2294
BstFNI	CGCG	3 cut(s)	870, 1544, 1808
BstH2I	RGCGCY	1 cut(s)	197
BstHHI	GCGC	3 cut(s)	196, 315, 1036
BstMAI	GTCTC	5 cut(s)	339, 732, 1024, 1508, 1573
BstMCI	CGRYCG	2 cut(s)	874, 1275
BstNI	CCWGG	3 cut(s)	233, 2007, 2702
BstNSI	RCATGY	2 cut(s)	1014, 1693
BstPAI	GACNNNNGTC	1 cut(s)	1379
BstSCI	CCNGG	3 cut(s)	231, 2005, 2700
BstSFI	CTRYAG	1 cut(s)	1836
BstUI	CGCG	3 cut(s)	870, 1544, 1808
BstV1I	GCAGC	3 cut(s)	19, 194, 197
BstV2I	GAAGAC	1 cut(s)	2462
BstX2I	RGATCY	7 cut(s)	1134, 1288, 1297, 1357, 1831, 1998, 2233
BstXI	CCANNNNNNTGG	2 cut(s)	299, 381
BstYI	RGATCY	7 cut(s)	1134, 1288, 1297, 1357, 1831, 1998, 2233
BstZI	CGGCCG	2 cut(s)	871, 1272
Bsu15I	ATCGAT	1 cut(s)	2805
BsuI	GTATCC	1 cut(s)	1206
BsuRI	GGCC	7 cut(s)	5, 867, 873, 1093, 1274, 2255, 2481
BsuTUI	ATCGAT	1 cut(s)	2805
BtgI	CCRYGG	5 cut(s)	292, 868, 1317, 2058, 2724
BtgZI	GCGATG	1 cut(s)	2507
BtsCI	GGATG	7 cut(s)	222, 386, 529, 799, 1230, 1963, 2294
BtsI	GCAGTG	4 cut(s)	959, 1423, 1657, 1914
BtsIMutI	CAGTG	6 cut(s)	204, 959, 1423, 1657, 1914, 2194
BveI	ACCTGC	1 cut(s)	2539
Cac8I	GCNNGC	6 cut(s)	58, 155, 442, 530, 1186, 2371
CaiI	CAGNNNCTG	3 cut(s)	206, 1310, 1439
CciI	TCATGA	1 cut(s)	504
CciNI	GCGGCCGC	1 cut(s)	871
CfoI	GCGC	3 cut(s)	196, 315, 1036
Cfr10I	RCCGGY	1 cut(s)	56
Cfr13I	GGNCC	4 cut(s)	4, 865, 2480, 2704
Cfr42I	CCGCGG	1 cut(s)	871
ClaI	ATCGAT	1 cut(s)	2805
CseI	GACGC	4 cut(s)	90, 159, 1600, 2650
Csp6I	GTAC	6 cut(s)	424, 451, 1072, 1810, 2642, 2721
CviQI	GTAC	6 cut(s)	424, 451, 1072, 1810, 2642, 2721
EaeI	YGGCCR	3 cut(s)	871, 1272, 2253
EagI	CGGCCG	2 cut(s)	871, 1272
Eam1104I	CTCTTC	4 cut(s)	1739, 1752, 1887, 2531
EarI	CTCTTC	4 cut(s)	1739, 1752, 1887, 2531
Ecl136II	GAGCTC	2 cut(s)	1590, 1963
EclXI	CGGCCG	2 cut(s)	871, 1272
Eco130I	CCWWGG	4 cut(s)	1317, 2058, 2112, 2724
Eco24I	GRGCYC	3 cut(s)	809, 1592, 1965
Eco31I	GGTCTC	2 cut(s)	339, 732
Eco47I	GGWCC	1 cut(s)	2704
Eco52I	CGGCCG	2 cut(s)	871, 1272
Eco53kI	GAGCTC	2 cut(s)	1590, 1963
Eco57I	CTGAAG	2 cut(s)	57, 2342
Eco88I	CYCGRG	1 cut(s)	8
EcoICRI	GAGCTC	2 cut(s)	1590, 1963
EcoNI	CCTNNNNNAGG	1 cut(s)	2111
EcoO109I	RGGNCCY	1 cut(s)	2704
EcoRI	GAATTC	1 cut(s)	500
EcoRII	CCWGG	3 cut(s)	231, 2005, 2700
EcoT14I	CCWWGG	4 cut(s)	1317, 2058, 2112, 2724
EcoT22I	ATGCAT	1 cut(s)	2412
EcoT38I	GRGCYC	3 cut(s)	809, 1592, 1965
ErhI	CCWWGG	4 cut(s)	1317, 2058, 2112, 2724
Esp3I	CGTCTC	1 cut(s)	1508
FalI	AAGNNNNNCTT	2 cut(s)	1881, 1913
FaqI	GGGAC	3 cut(s)	382, 1335, 2407
FauI	CCCGC	1 cut(s)	2433
FbaI	TGATCA	2 cut(s)	1764, 2815
Fnu4HI	GCNGC	8 cut(s)	33, 183, 186, 868, 871, 874, 1272, 1542
FokI	GGATG	7 cut(s)	209, 393, 536, 806, 1237, 1970, 2301
FriOI	GRGCYC	3 cut(s)	809, 1592, 1965
Fsp4HI	GCNGC	8 cut(s)	33, 183, 186, 868, 871, 874, 1272, 1542
FspBI	CTAG	6 cut(s)	113, 165, 1980, 2387, 2396, 2753
GlaI	GCGC	3 cut(s)	195, 314, 1035
GluI	GCNGC	8 cut(s)	33, 183, 186, 868, 871, 874, 1272, 1542
GsaI	CCCAGC	1 cut(s)	483
GsuI	CTGGAG	4 cut(s)	215, 1950, 2260, 2590
HaeII	RGCGCY	1 cut(s)	197
HaeIII	GGCC	7 cut(s)	5, 867, 873, 1093, 1274, 2255, 2481
HapII	CCGG	2 cut(s)	57, 2665
HgaI	GACGC	4 cut(s)	90, 159, 1600, 2650
HhaI	GCGC	3 cut(s)	196, 315, 1036
Hin1I	GRCGYC	3 cut(s)	151, 1611, 2661
Hin6I	GCGC	3 cut(s)	194, 313, 1034
HinP1I	GCGC	3 cut(s)	194, 313, 1034
HincII	GTYRAC	1 cut(s)	754
HindII	GTYRAC	1 cut(s)	754
HindIII	AAGCTT	2 cut(s)	969, 2037
HinfI	GANTC	5 cut(s)	516, 1323, 1430, 2420, 2546
HpaII	CCGG	2 cut(s)	57, 2665
Hpy166II	GTNNAC	7 cut(s)	453, 754, 1429, 1697, 2349, 2440, 2455
Hpy8I	GTNNAC	7 cut(s)	453, 754, 1429, 1697, 2349, 2440, 2455
Hpy99I	CGWCG	1 cut(s)	1667
Hsp92I	GRCGYC	3 cut(s)	151, 1611, 2661
HspAI	GCGC	3 cut(s)	194, 313, 1034
Kpn2I	TCCGGA	1 cut(s)	2664
KroI	GCCGGC	1 cut(s)	56
KroNI	GCCGGC	1 cut(s)	58
Ksp22I	TGATCA	2 cut(s)	1764, 2815
KspI	CCGCGG	1 cut(s)	871
LguI	GCTCTTC	1 cut(s)	1887
LmnI	GCTCC	4 cut(s)	639, 1587, 1990, 2241
Lsp1109I	GCAGC	3 cut(s)	19, 194, 197
LweI	GCATC	6 cut(s)	324, 909, 1215, 1493, 1682, 2397
MaeI	CTAG	6 cut(s)	113, 165, 1980, 2387, 2396, 2753
MaeIII	GTNAC	8 cut(s)	883, 1312, 1535, 2153, 2189, 2382, 2389, 2499
MflI	RGATCY	7 cut(s)	1134, 1288, 1297, 1357, 1831, 1998, 2233
MhlI	GDGCHC	4 cut(s)	809, 1592, 1965, 2246
MlsI	TGGCCA	1 cut(s)	2255
MluI	ACGCGT	1 cut(s)	1806
MluNI	TGGCCA	1 cut(s)	2255
MlyI	GAGTC	5 cut(s)	525, 1317, 1424, 2429, 2555
MmeI	TCCRAC	1 cut(s)	1932
Mox20I	TGGCCA	1 cut(s)	2255
Mph1103I	ATGCAT	1 cut(s)	2412
MroI	TCCGGA	1 cut(s)	2664
MroNI	GCCGGC	1 cut(s)	56
MroXI	GAANNNNTTC	2 cut(s)	1148, 1171
MscI	TGGCCA	1 cut(s)	2255
MseI	TTAA	7 cut(s)	138, 861, 1412, 1899, 2141, 2204, 2709
MslI	CAYNNNNRTG	8 cut(s)	297, 303, 537, 557, 881, 1704, 2249, 2306
Msp20I	TGGCCA	1 cut(s)	2255
MspA1I	CMGCKG	2 cut(s)	479, 870
MspI	CCGG	2 cut(s)	57, 2665
MspR9I	CCNGG	3 cut(s)	233, 2007, 2702
Mva1269I	GAATGC	2 cut(s)	994, 2165
MvaI	CCWGG	3 cut(s)	233, 2007, 2702
MvnI	CGCG	3 cut(s)	870, 1544, 1808
NaeI	GCCGGC	1 cut(s)	58
NcoI	CCATGG	3 cut(s)	1317, 2058, 2724
NgoMIV	GCCGGC	1 cut(s)	56
NlaIV	GGNNCC	4 cut(s)	43, 91, 352, 2509
NmeAIII	GCCGAG	1 cut(s)	940
NmuCI	GTSAC	4 cut(s)	883, 1312, 2153, 2189
NotI	GCGGCCGC	1 cut(s)	871
NsiI	ATGCAT	1 cut(s)	2412
NspI	RCATGY	2 cut(s)	1014, 1693
PaeR7I	CTCGAG	1 cut(s)	8
PagI	TCATGA	1 cut(s)	504
PciSI	GCTCTTC	1 cut(s)	1887
PctI	GAATGC	2 cut(s)	994, 2165
PdiI	GCCGGC	1 cut(s)	58
PdmI	GAANNNNTTC	2 cut(s)	1148, 1171
PflFI	GACNNNGTC	1 cut(s)	2545
PfoI	TCCNGGA	1 cut(s)	231
PkrI	GCNGC	8 cut(s)	34, 184, 187, 869, 872, 875, 1273, 1543
PleI	GAGTC	5 cut(s)	524, 1317, 1424, 2428, 2554
PpsI	GAGTC	5 cut(s)	524, 1317, 1424, 2428, 2554
PpuMI	RGGWCCY	1 cut(s)	2704
PshAI	GACNNNNGTC	1 cut(s)	1379
PsiI	TTATAA	2 cut(s)	2276, 2555
Psp124BI	GAGCTC	2 cut(s)	1592, 1965
Psp5II	RGGWCCY	1 cut(s)	2704
Psp6I	CCWGG	3 cut(s)	231, 2005, 2700
PspFI	CCCAGC	1 cut(s)	479
PspGI	CCWGG	3 cut(s)	231, 2005, 2700
PspN4I	GGNNCC	4 cut(s)	43, 91, 352, 2509
PspPI	GGNCC	4 cut(s)	4, 865, 2480, 2704
PspPPI	RGGWCCY	1 cut(s)	2704
PstNI	CAGNNNCTG	3 cut(s)	206, 1310, 1439
PsuI	RGATCY	7 cut(s)	1134, 1288, 1297, 1357, 1831, 1998, 2233
PsyI	GACNNNGTC	1 cut(s)	2545
PvuII	CAGCTG	1 cut(s)	479
RsaI	GTAC	6 cut(s)	425, 452, 1073, 1811, 2643, 2722
RsaNI	GTAC	6 cut(s)	424, 451, 1072, 1810, 2642, 2721
RseI	CAYNNNNRTG	8 cut(s)	297, 303, 537, 557, 881, 1704, 2249, 2306
SacI	GAGCTC	2 cut(s)	1592, 1965
SacII	CCGCGG	1 cut(s)	871
SapI	GCTCTTC	1 cut(s)	1887
SaqAI	TTAA	7 cut(s)	138, 861, 1412, 1899, 2141, 2204, 2709
SatI	GCNGC	8 cut(s)	33, 183, 186, 868, 871, 874, 1272, 1542
Sau96I	GGNCC	4 cut(s)	4, 865, 2480, 2704
SchI	GAGTC	5 cut(s)	525, 1317, 1424, 2429, 2555
ScrFI	CCNGG	3 cut(s)	233, 2007, 2702
SduI	GDGCHC	4 cut(s)	809, 1592, 1965, 2246
SfaNI	GCATC	6 cut(s)	324, 909, 1215, 1493, 1682, 2397
SfcI	CTRYAG	1 cut(s)	1836
Sfr274I	CTCGAG	1 cut(s)	8
Sfr303I	CCGCGG	1 cut(s)	871
SgrBI	CCGCGG	1 cut(s)	871
SinI	GGWCC	1 cut(s)	2704
SlaI	CTCGAG	1 cut(s)	8
SmiMI	CAYNNNNRTG	8 cut(s)	297, 303, 537, 557, 881, 1704, 2249, 2306
SmlI	CTYRAG	4 cut(s)	8, 1161, 1451, 2296
SmoI	CTYRAG	4 cut(s)	8, 1161, 1451, 2296
SpeI	ACTAGT	2 cut(s)	2386, 2752
SsiI	CCGC	9 cut(s)	598, 868, 870, 874, 1271, 1482, 1542, 2367, 2426
SspI	AATATT	2 cut(s)	1683, 1732
SspMI	CTAG	6 cut(s)	113, 165, 1980, 2387, 2396, 2753
SstI	GAGCTC	2 cut(s)	1592, 1965
StyD4I	CCNGG	3 cut(s)	231, 2005, 2700
StyI	CCWWGG	4 cut(s)	1317, 2058, 2112, 2724
TaqI	TCGA	8 cut(s)	9, 259, 498, 1022, 1555, 1629, 1967, 2805
TaqII	GACCGA	1 cut(s)	1859
TatI	WGTACW	1 cut(s)	423
TauI	GCSGC	5 cut(s)	870, 873, 876, 1274, 1544
Tru1I	TTAA	7 cut(s)	138, 861, 1412, 1899, 2141, 2204, 2709
Tru9I	TTAA	7 cut(s)	138, 861, 1412, 1899, 2141, 2204, 2709
TscAI	CASTG	6 cut(s)	211, 966, 1423, 1664, 1914, 2194
TseFI	GTSAC	4 cut(s)	883, 1312, 2153, 2189
TseI	GCWGC	3 cut(s)	32, 182, 185
Tsp45I	GTSAC	4 cut(s)	883, 1312, 2153, 2189
TspDTI	ATGAA	9 cut(s)	186, 477, 493, 521, 713, 754, 1133, 2318, 2666
TspGWI	ACGGA	7 cut(s)	35, 309, 1391, 1680, 2036, 2301, 2596
TspRI	CASTG	6 cut(s)	211, 966, 1423, 1664, 1914, 2194
Tth111I	GACNNNGTC	1 cut(s)	2545
VpaK11BI	GGWCC	1 cut(s)	2704
XagI	CCTNNNNNAGG	1 cut(s)	2111
XapI	RAATTY	4 cut(s)	500, 1120, 1388, 1751
XceI	RCATGY	2 cut(s)	1014, 1693
XcmI	CCANNNNNNNNNTGG	2 cut(s)	299, 2065
XhoI	CTCGAG	1 cut(s)	8
XmnI	GAANNNNTTC	2 cut(s)	1148, 1171
XspI	CTAG	6 cut(s)	113, 165, 1980, 2387, 2396, 2753
Zsp2I	ATGCAT	1 cut(s)	2412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.