Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

69239597 .. 69243000

Gene Structure 3404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G042880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGTACCTGCAGCCAAACAGTACCAATGGTGATGAGGCCTCTGAGGGCATCAGCAACCCGACACTTACTACTGATCTTATCATTCAAACAGTTAGTAGTTGTGTCCAATATAAGCAGAGCCGCTGGCATAAATACATTGCTATTGTGGTTTTCATCTGGCGCTCCAGAACCAAGCCACGCCTCCGCAGAGCAAAAGCCCCTCATCATCAAAGGTTTCTGAACAAGGTTGTTCGCCTTGATGTGAGACAGAAGGTCTCAGACCTCGCGTCCTACTTCAAGGATCGTGCTGCCTATATCCCTTTGTTAGCACCAACAACACCAACAACGCCGCCAGCCGCTGCCTCAAATCATGTGATTGACATCCCTCTCCAAGATCTGAAAGAGGTTGCTCGCATTGTGAGAGTGAAGGACTTGAATGCACTGCGAGCACGCGGTGGTGTTGATGGTGTTTCAACAGTACTTCTCAGGTCTCACTCTGAGGATCATGCTGATGATGGTGGTCAAGTTCCACAACTAGCATGCAACCCTGCCAAGCCTGTTTTCTTTCACTTCTTCTTGAAGGCTTGCAATCACTACACAATCTTTTTCCTCTTGGGCTCAGCGGGTTTGCAATTTGCTATTGAATTCATGAAGCAAGGAGTCAAACAAGGGTGGCATGACGGTGTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTTCCTTCAGTTGGGAACTATCGCAACGAGAGGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTTCTGGTGGATGTTGAAAGAAATGGAGAATCTAGAGCGGTTATGATATCTCATGTTTCGGTGGGTGATATAGTTCATTTGAAGAAGGGAGACCATGCTCCTGCTGATGGCTTGTTCATAGATCATGGTGTGGAACTGGTCTTGGATGAGGTATTGAGCCCCACAATTGATTTTGAAAACAACCCATTTGTGTTGTCTGGTTCAAAAGTTATTGAGGGCCAGGGTCGAATGATTGTGACATCTGTTGGTGTCGATACAGCTTTTGCAGAGATGCTGAGCTTGGTGACTGAGGATCCTAATCCAAATAGGAAGACTCTGTTACAAGCTTTGATGGACAAACCATATACTTATATGGAGTATCTCGCATGTTGTGTTTCAATTCTGATTGCTTTTGTGGTCCTAATACGGTTTCTAGTATTCAGGAAGCATGACAACTATAATGACAGGCCAGACCTGAAGGGGGAAGTTTCAATGAACTTGCTGATGAAGATTTTCGAGAAAATTTACTTCAAAACTCAAGGAAGGGTCTCCACTTTAGCAAGTGTTCTTGCTACTGTGGTAATTGGGATACAACATGGAATGCCCTTTGTGATTTCCCTTGCACTTTGTCAATGGAAGGAGAAGGTGGTCCAAAATGGGGTAGATCCTCGGAATCTTTCAGCTTGTGTCACCATGGGACTCATAACAGTCATCTGCATTGAAACAACTAGTGAGAAAATGTGTAACCAGATGGAGGTTATGGACTTTTGGATGGGTGATAAGGATTTAAGCAATGAAGTGGAGTCTGATCAAACTGAACAATTTGCTCTCAAAGGACTACGTCAAGAGATTTCTGCTACCCCAGCAAATGATTCGTTCATTTCTTCGATCAAGGCCAGATGGGGCGTCACTGACATGGAGGTATTGGATCAGACATTTATAACTCTGGAAGAGAGAAAATTGAGTTCTGATGAGAAATGTAGTGGCATCCTAATGAGGAAAACAGAGAGCAGTGCACAAGATATGCAGCTGCATATGAGTGGAGATGCATCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACAGGGGAAAGTCGTATTCTGGAAATTCAGAAGAGAAAGTTTAAGCAGGTCATTAAGAACATGGAGGAGAAGGGTCTTAGACCGATTGCATATGCTTACAAGAAAACAAATGTTCAAGAATTTACAGAAAATGGGTTGAAATTGTTGGCACTGGTGGGAGTCAGATGCCCATATCAGGAAGAGCTTAAAATTGCAGTGGAAGCTTTTAGAAAGGCCGGAATAAGAATCAAGCTGGTATCCGAGGATGAGCTCTCAATAGTGACTGAGCGAGCTTCTGATCTTGGAATCTTTAACCCTGGTAGTGGTGATATGGAGATCATCGCAGGCGAAGCTTTCAGAAGACTCAACTCCATGGAAAGACTAAATAAGGTGGATTTGATATCTGTGATGGGAAGTTCTCTCCCAAGAGACAAGCTTCTCATGGTGGAGCGCTTAAAGAAAAACGGTCACATAGTAGCGTTCTATGGAGGGTTAACCATAAATGATACAAGGACTCTGAAAGAAGCTGATGTTGGAATCACAGAGGACATATGGAGCACTGAGATGGCCAGAGACAATGCTGATATTATAGTTAAGGATGGCTGCTCATGGTTCCTGAACTTGAAGTCTGGGAAATGTGCTTACCACAACATTCAGAACTTCTGTCAAATTCAGCTCACGGCTTGCATATCCGGGTTGCTAGTAACGTTAGTAGCAACGATGCATTCAGGGGACTCTCCATTGACCGCAATTCACTTGATTTGGGTGAACTTGATAATGTGCCTTCTAGGCAGCCTAATGATGGTAATGGAGTTAGAACGCCAGGAGCCACTTGCACAAGAACCAGCGCCAAGGACTCAAGAACCGGCCCCAAGTACTCAAGAACCGGCACGGACTCAGCCACTTATAACCAAAGCCATCTGGGGAAACATAGCCAGTCAAGTTTCATACCAGGCTTCCATCTTGCTGGTCTTGCATTTCATGGGAAATGCAATAGCGAGCATGAACCAAGGCGTCCGGGGTACCATGGTTTTCAATATTTACACCTTGTGTCAGGTCCTTAATCTGTTCAGGGCTATGGACCTAGTGAACAAGGAAGTGCTAAATGTTGTTCTTCACAGCTATTGGTTTTTGATGGCCTTGGGGGCTGTTATGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGACTAGCCAGTGGTGTGAGGTTGGATGCAATACATTGGGCATCCTGCTTCCTTCTTGCTGCTCTTTCATGGCCGTTTGATTGGGCCATAACTTCGCAGTTTCGAAGAGAAAATTTGGCATGGAGGAGAGCACCTATGGGGATGTCAAGATTTTACATTTGTGCTTCGTTGTTCCTCATTTTCTCTGTATCATATTGTTTCGAATCAGATTATGCACACACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1075

Amino Acids

120.53

Weight (kDa)

7.1

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	258 - 350	4e-09	P-type ATPase actuator domain
Cation_ATPase	PF13246	524 - 670	1e-10	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	850 - 1029	2.8e-17	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1685, 1824, 2720
AarI	CACCTGC	1 cut(s)	2965
Acc36I	ACCTGC	3 cut(s)	15, 1870, 2965
Acc65I	GGTACC	1 cut(s)	2834
AccB1I	GGYRCC	1 cut(s)	2834
AccBSI	CCGCTC	1 cut(s)	803
AccII	CGCG	2 cut(s)	266, 432
AciI	CCGC	8 cut(s)	121, 184, 329, 336, 432, 602, 803, 2559
AclI	AACGTT	1 cut(s)	2519
AclWI	GGATC	6 cut(s)	288, 489, 1052, 1065, 1403, 1680
AcoI	YGGCCR	2 cut(s)	2379, 3071
AcsI	RAATTY	7 cut(s)	623, 764, 1266, 1857, 1952, 2481, 3112
AcuI	CTGAAG	2 cut(s)	690, 1241
AcyI	GRCGYC	2 cut(s)	1650, 2826
AdeI	CACNNNGTG	2 cut(s)	434, 2862
AfaI	GTAC	5 cut(s)	5, 22, 459, 2689, 2836
AfeI	AGCGCT	1 cut(s)	2264
AfiI	CCNNNNNNNGG	8 cut(s)	710, 872, 1225, 1402, 1498, 2008, 2135, 2614
AflIII	ACRYGT	1 cut(s)	1809
AhdI	GACNNNNNGTC	2 cut(s)	251, 265
AhlI	ACTAGT	1 cut(s)	1472
AjnI	CCWGG	4 cut(s)	984, 2128, 2634, 2763
AjuI	GAANNNNNNNTTGG	4 cut(s)	524, 556, 2328, 2360
Alw21I	GWGCWC	5 cut(s)	430, 1762, 2085, 2372, 3133
Alw26I	GTCTC	7 cut(s)	238, 259, 474, 849, 1297, 2235, 2379
Alw44I	GTGCAC	1 cut(s)	1758
AlwI	GGATC	6 cut(s)	288, 489, 1052, 1065, 1403, 1680
AlwNI	CAGNNNCTG	1 cut(s)	338
Aor51HI	AGCGCT	1 cut(s)	2264
ApaLI	GTGCAC	1 cut(s)	1758
ApeKI	GCWGC	8 cut(s)	10, 287, 338, 1771, 1774, 2415, 2604, 3059
ApoI	RAATTY	7 cut(s)	623, 764, 1266, 1857, 1952, 2481, 3112
Asp700I	GAANNNNTTC	1 cut(s)	1256
Asp718I	GGTACC	1 cut(s)	2834
AspLEI	GCGC	3 cut(s)	162, 2265, 2662
AspS9I	GGNCC	7 cut(s)	982, 1162, 1393, 2680, 2869, 2893, 3084
AsuC2I	CCSGG	2 cut(s)	2506, 2831
AsuHPI	GGTGA	9 cut(s)	41, 751, 842, 1060, 1426, 1532, 2150, 2590, 2992
AsuII	TTCGAA	2 cut(s)	3103, 3201
AvaII	GGWCC	4 cut(s)	1162, 1393, 2869, 2893
BaeGI	GKGCMC	1 cut(s)	1762
BaeI	ACNNNNGTAYC	2 cut(s)	2826, 2859
BalI	TGGCCA	1 cut(s)	2381
BamHI	GGATCC	1 cut(s)	1057
BanI	GGYRCC	1 cut(s)	2834
BanII	GRGCYC	3 cut(s)	599, 926, 2085
BbsI	GAAGAC	2 cut(s)	1082, 2179
Bbv12I	GWGCWC	5 cut(s)	430, 1762, 2085, 2372, 3133
BbvI	GCAGC	8 cut(s)	22, 274, 325, 1761, 1783, 2402, 2616, 3046
BceAI	ACGGC	2 cut(s)	2508, 3058
BcgI	CGANNNNNNTGC	2 cut(s)	1599, 1633
BciT130I	CCWGG	4 cut(s)	986, 2130, 2636, 2765
BciVI	GTATCC	2 cut(s)	1326, 2080
BclI	TGATCA	1 cut(s)	1552
BcnI	CCSGG	2 cut(s)	2506, 2831
BcoDI	GTCTC	7 cut(s)	238, 259, 474, 849, 1297, 2235, 2379
BcuI	ACTAGT	1 cut(s)	1472
BfaI	CTAG	8 cut(s)	515, 798, 1178, 1473, 2513, 2600, 2897, 3005
BfmI	CTRYAG	1 cut(s)	8
BfoI	RGCGCY	3 cut(s)	163, 2266, 2663
BfuAI	ACCTGC	3 cut(s)	15, 1870, 2965
BfuI	GTATCC	2 cut(s)	1326, 2080
BglI	GCCNNNNNGGC	2 cut(s)	2601, 2957
BglII	AGATCT	1 cut(s)	373
BlpI	GCTNAGC	2 cut(s)	598, 1040
BmcAI	AGTACT	2 cut(s)	459, 2689
Bme1390I	CCNGG	6 cut(s)	986, 2130, 2506, 2636, 2765, 2831
Bme18I	GGWCC	4 cut(s)	1162, 1393, 2869, 2893
BmeRI	GACNNNNNGTC	2 cut(s)	251, 265
BmgT120I	GGNCC	7 cut(s)	982, 1162, 1393, 2680, 2869, 2893, 3084
BmiI	GGNNCC	5 cut(s)	1059, 2426, 2640, 2682, 2836
BmrFI	CCNGG	6 cut(s)	986, 2130, 2506, 2636, 2765, 2831
BmsI	GCATC	9 cut(s)	57, 1026, 1740, 1780, 1802, 1988, 2523, 3016, 3050
BpiI	GAAGAC	2 cut(s)	1082, 2179
BpmI	CTGGAG	1 cut(s)	148
Bpu1102I	GCTNAGC	2 cut(s)	598, 1040
Bpu14I	TTCGAA	2 cut(s)	3103, 3201
BpuEI	CTTGAG	3 cut(s)	1266, 2655, 2676
BpuMI	CCSGG	2 cut(s)	2506, 2831
BsaBI	GATNNNNATC	2 cut(s)	359, 1062
BsaHI	GRCGYC	2 cut(s)	1650, 2826
BsaI	GGTCTC	4 cut(s)	259, 474, 849, 1297
Bsc4I	CCNNNNNNNGG	8 cut(s)	710, 872, 1225, 1402, 1498, 2008, 2135, 2614
Bse118I	RCCGGY	2 cut(s)	2677, 2698
Bse1I	ACTGG	4 cut(s)	906, 1989, 2748, 3009
Bse3DI	GCAATG	2 cut(s)	135, 1543
Bse8I	GATNNNNATC	2 cut(s)	359, 1062
BseBI	CCWGG	4 cut(s)	986, 2130, 2636, 2765
BseJI	GATNNNNATC	2 cut(s)	359, 1062
BseLI	CCNNNNNNNGG	8 cut(s)	710, 872, 1225, 1402, 1498, 2008, 2135, 2614
BseMI	GCAATG	2 cut(s)	135, 1543
BseNI	ACTGG	4 cut(s)	906, 1989, 2748, 3009
BseRI	GAGGAG	2 cut(s)	1913, 3139
BseSI	GKGCMC	1 cut(s)	1762
BseXI	GCAGC	8 cut(s)	22, 274, 325, 1761, 1783, 2402, 2616, 3046
BseYI	CCCAGC	1 cut(s)	1606
Bsh1236I	CGCG	2 cut(s)	266, 432
BshNI	GGYRCC	1 cut(s)	2834
BsiHKAI	GWGCWC	5 cut(s)	430, 1762, 2085, 2372, 3133
BsiSI	CCGG	5 cut(s)	2049, 2505, 2678, 2699, 2830
BslFI	GGGAC	2 cut(s)	1455, 2558
BslI	CCNNNNNNNGG	8 cut(s)	710, 872, 1225, 1402, 1498, 2008, 2135, 2614
BsmAI	GTCTC	7 cut(s)	238, 259, 474, 849, 1297, 2235, 2379
BsmFI	GGGAC	2 cut(s)	1455, 2558
BsmI	GAATGC	3 cut(s)	421, 1350, 2536
Bso31I	GGTCTC	4 cut(s)	259, 474, 849, 1297
Bsp119I	TTCGAA	2 cut(s)	3103, 3201
Bsp1286I	GDGCHC	7 cut(s)	430, 599, 926, 1762, 2085, 2372, 3133
Bsp1720I	GCTNAGC	2 cut(s)	598, 1040
Bsp19I	CCATGG	3 cut(s)	1437, 2184, 2838
BspACI	CCGC	8 cut(s)	121, 184, 329, 336, 432, 602, 803, 2559
BspFNI	CGCG	2 cut(s)	266, 432
BspHI	TCATGA	1 cut(s)	627
BspLI	GGNNCC	5 cut(s)	1059, 2426, 2640, 2682, 2836
BspMAI	CTGCAG	1 cut(s)	12
BspMI	ACCTGC	3 cut(s)	15, 1870, 2965
BspPI	GGATC	6 cut(s)	288, 489, 1052, 1065, 1403, 1680
BspQI	GCTCTTC	1 cut(s)	2007
BspT104I	TTCGAA	2 cut(s)	3103, 3201
BspT107I	GGYRCC	1 cut(s)	2834
BspTNI	GGTCTC	4 cut(s)	259, 474, 849, 1297
BsrBI	CCGCTC	1 cut(s)	803
BsrDI	GCAATG	2 cut(s)	135, 1543
BsrFI	RCCGGY	2 cut(s)	2677, 2698
BsrI	ACTGG	4 cut(s)	906, 1989, 2748, 3009
BssAI	RCCGGY	2 cut(s)	2677, 2698
BssNI	GRCGYC	2 cut(s)	1650, 2826
BssT1I	CCWWGG	6 cut(s)	1437, 2184, 2663, 2821, 2838, 2952
Bst2UI	CCWGG	4 cut(s)	986, 2130, 2636, 2765
Bst4CI	ACNGT	9 cut(s)	20, 91, 457, 662, 682, 1173, 1321, 1453, 2279
Bst6I	CTCTTC	4 cut(s)	1689, 1859, 2007, 3100
BstACI	GRCGYC	2 cut(s)	1650, 2826
BstBI	TTCGAA	2 cut(s)	3103, 3201
BstDSI	CCRYGG	3 cut(s)	1437, 2184, 2838
BstFNI	CGCG	2 cut(s)	266, 432
BstH2I	RGCGCY	3 cut(s)	163, 2266, 2663
BstHHI	GCGC	3 cut(s)	162, 2265, 2662
BstMAI	GTCTC	7 cut(s)	238, 259, 474, 849, 1297, 2235, 2379
BstMWI	GCNNNNNNNGC	7 cut(s)	425, 1607, 2033, 2162, 2601, 2785, 2957
BstNI	CCWGG	4 cut(s)	986, 2130, 2636, 2765
BstNSI	RCATGY	3 cut(s)	522, 1134, 1813
BstSCI	CCNGG	6 cut(s)	984, 2128, 2504, 2634, 2763, 2829
BstSFI	CTRYAG	1 cut(s)	8
BstSLI	GKGCMC	1 cut(s)	1762
BstUI	CGCG	2 cut(s)	266, 432
BstV1I	GCAGC	8 cut(s)	22, 274, 325, 1761, 1783, 2402, 2616, 3046
BstV2I	GAAGAC	2 cut(s)	1082, 2179
BstX2I	RGATCY	3 cut(s)	373, 1057, 1408
BstXI	CCANNNNNNTGG	1 cut(s)	2779
BstYI	RGATCY	3 cut(s)	373, 1057, 1408
BsuI	GTATCC	2 cut(s)	1326, 2080
BtgI	CCRYGG	3 cut(s)	1437, 2184, 2838
BtgZI	GCGATG	1 cut(s)	2137
BtsI	GCAGTG	3 cut(s)	419, 1762, 2034
BtsIMutI	CAGTG	7 cut(s)	419, 1653, 1762, 1982, 2034, 2370, 3016
BveI	ACCTGC	3 cut(s)	15, 1870, 2965
CaiI	CAGNNNCTG	1 cut(s)	338
CciI	TCATGA	1 cut(s)	627
CfoI	GCGC	3 cut(s)	162, 2265, 2662
Cfr10I	RCCGGY	2 cut(s)	2677, 2698
Cfr13I	GGNCC	7 cut(s)	982, 1162, 1393, 2680, 2869, 2893, 3084
CseI	GACGC	3 cut(s)	255, 1639, 2815
Csp6I	GTAC	5 cut(s)	4, 21, 458, 2688, 2835
CviQI	GTAC	5 cut(s)	4, 21, 458, 2688, 2835
DraIII	CACNNNGTG	2 cut(s)	434, 2862
DriI	GACNNNNNGTC	2 cut(s)	251, 265
EaeI	YGGCCR	2 cut(s)	2379, 3071
Eam1104I	CTCTTC	4 cut(s)	1689, 1859, 2007, 3100
Eam1105I	GACNNNNNGTC	2 cut(s)	251, 265
EarI	CTCTTC	4 cut(s)	1689, 1859, 2007, 3100
Ecl136II	GAGCTC	1 cut(s)	2083
Eco130I	CCWWGG	6 cut(s)	1437, 2184, 2663, 2821, 2838, 2952
Eco147I	AGGCCT	1 cut(s)	38
Eco24I	GRGCYC	3 cut(s)	599, 926, 2085
Eco31I	GGTCTC	4 cut(s)	259, 474, 849, 1297
Eco32I	GATATC	2 cut(s)	813, 2214
Eco47I	GGWCC	4 cut(s)	1162, 1393, 2869, 2893
Eco47III	AGCGCT	1 cut(s)	2264
Eco53kI	GAGCTC	1 cut(s)	2083
Eco57I	CTGAAG	2 cut(s)	690, 1241
EcoICRI	GAGCTC	1 cut(s)	2083
EcoO109I	RGGNCCY	1 cut(s)	2869
EcoRI	GAATTC	1 cut(s)	623
EcoRII	CCWGG	4 cut(s)	984, 2128, 2634, 2763
EcoRV	GATATC	2 cut(s)	813, 2214
EcoT14I	CCWWGG	6 cut(s)	1437, 2184, 2663, 2821, 2838, 2952
EcoT22I	ATGCAT	2 cut(s)	1795, 2538
EcoT38I	GRGCYC	3 cut(s)	599, 926, 2085
ErhI	CCWWGG	6 cut(s)	1437, 2184, 2663, 2821, 2838, 2952
FaqI	GGGAC	2 cut(s)	1455, 2558
FauI	CCCGC	1 cut(s)	595
FauNDI	CATATG	3 cut(s)	1779, 1924, 2363
FbaI	TGATCA	1 cut(s)	1552
FriOI	GRGCYC	3 cut(s)	599, 926, 2085
FspBI	CTAG	8 cut(s)	515, 798, 1178, 1473, 2513, 2600, 2897, 3005
GlaI	GCGC	3 cut(s)	161, 2264, 2661
GsaI	CCCAGC	1 cut(s)	1610
GsuI	CTGGAG	1 cut(s)	148
HaeII	RGCGCY	3 cut(s)	163, 2266, 2663
HapII	CCGG	5 cut(s)	2049, 2505, 2678, 2699, 2830
HgaI	GACGC	3 cut(s)	255, 1639, 2815
HhaI	GCGC	3 cut(s)	162, 2265, 2662
Hin1I	GRCGYC	2 cut(s)	1650, 2826
Hin6I	GCGC	3 cut(s)	160, 2263, 2660
HinP1I	GCGC	3 cut(s)	160, 2263, 2660
HincII	GTYRAC	1 cut(s)	2307
HindII	GTYRAC	1 cut(s)	2307
HindIII	AAGCTT	4 cut(s)	1089, 2034, 2163, 2246
HpaI	GTTAAC	1 cut(s)	2307
HpaII	CCGG	5 cut(s)	2049, 2505, 2678, 2699, 2830
HphI	GGTGA	9 cut(s)	41, 751, 842, 1060, 1426, 1532, 2150, 2590, 2992
Hpy166II	GTNNAC	5 cut(s)	1760, 1817, 2307, 2581, 2902
Hpy8I	GTNNAC	5 cut(s)	1760, 1817, 2307, 2581, 2902
HpyCH4III	ACNGT	9 cut(s)	20, 91, 457, 662, 682, 1173, 1321, 1453, 2279
HpyCH4IV	ACGT	2 cut(s)	1585, 2519
HpyF10VI	GCNNNNNNNGC	7 cut(s)	425, 1607, 2033, 2162, 2601, 2785, 2957
HpySE526I	ACGT	2 cut(s)	1585, 2519
Hsp92I	GRCGYC	2 cut(s)	1650, 2826
HspAI	GCGC	3 cut(s)	160, 2263, 2660
KpnI	GGTACC	1 cut(s)	2838
Ksp22I	TGATCA	1 cut(s)	1552
KspAI	GTTAAC	1 cut(s)	2307
LguI	GCTCTTC	1 cut(s)	2007
LmnI	GCTCC	5 cut(s)	167, 868, 2260, 2367, 2638
Lsp1109I	GCAGC	8 cut(s)	22, 274, 325, 1761, 1783, 2402, 2616, 3046
LweI	GCATC	9 cut(s)	57, 1026, 1740, 1780, 1802, 1988, 2523, 3016, 3050
MaeI	CTAG	8 cut(s)	515, 798, 1178, 1473, 2513, 2600, 2897, 3005
MaeII	ACGT	2 cut(s)	1585, 2519
MbiI	CCGCTC	1 cut(s)	803
MfeI	CAATTG	1 cut(s)	930
MflI	RGATCY	3 cut(s)	373, 1057, 1408
MhlI	GDGCHC	7 cut(s)	430, 599, 926, 1762, 2085, 2372, 3133
MlsI	TGGCCA	1 cut(s)	2381
MluNI	TGGCCA	1 cut(s)	2381
MmeI	TCCRAC	2 cut(s)	2326, 3003
Mox20I	TGGCCA	1 cut(s)	2381
Mph1103I	ATGCAT	2 cut(s)	1795, 2538
MroXI	GAANNNNTTC	1 cut(s)	1256
MscI	TGGCCA	1 cut(s)	2381
MseI	TTAA	9 cut(s)	1532, 1875, 1887, 2019, 2124, 2267, 2306, 2406, 2874
MslI	CAYNNNNRTG	8 cut(s)	489, 660, 1658, 1736, 1782, 1824, 2375, 2975
Msp20I	TGGCCA	1 cut(s)	2381
MspA1I	CMGCKG	4 cut(s)	123, 338, 602, 1774
MspI	CCGG	5 cut(s)	2049, 2505, 2678, 2699, 2830
MspR9I	CCNGG	6 cut(s)	986, 2130, 2506, 2636, 2765, 2831
MunI	CAATTG	1 cut(s)	930
Mva1269I	GAATGC	3 cut(s)	421, 1350, 2536
MvaI	CCWGG	4 cut(s)	986, 2130, 2636, 2765
MvnI	CGCG	2 cut(s)	266, 432
MwoI	GCNNNNNNNGC	7 cut(s)	425, 1607, 2033, 2162, 2601, 2785, 2957
NciI	CCSGG	2 cut(s)	2506, 2831
NcoI	CCATGG	3 cut(s)	1437, 2184, 2838
NdeI	CATATG	3 cut(s)	1779, 1924, 2363
NlaIV	GGNNCC	5 cut(s)	1059, 2426, 2640, 2682, 2836
NmuCI	GTSAC	6 cut(s)	1000, 1048, 1432, 1651, 2092, 2279
NsiI	ATGCAT	2 cut(s)	1795, 2538
NspI	RCATGY	3 cut(s)	522, 1134, 1813
NspV	TTCGAA	2 cut(s)	3103, 3201
PaeI	GCATGC	1 cut(s)	522
PagI	TCATGA	1 cut(s)	627
PaqCI	CACCTGC	1 cut(s)	2965
PceI	AGGCCT	1 cut(s)	38
PciI	ACATGT	1 cut(s)	1809
PciSI	GCTCTTC	1 cut(s)	2007
PctI	GAATGC	3 cut(s)	421, 1350, 2536
PdmI	GAANNNNTTC	1 cut(s)	1256
PfeI	GAWTC	7 cut(s)	794, 1417, 1616, 2058, 2118, 2349, 3203
PflFI	GACNNNGTC	1 cut(s)	1584
PpuMI	RGGWCCY	1 cut(s)	2869
PscI	ACATGT	1 cut(s)	1809
PsiI	TTATAA	3 cut(s)	1685, 1824, 2720
Psp124BI	GAGCTC	1 cut(s)	2085
Psp1406I	AACGTT	1 cut(s)	2519
Psp5II	RGGWCCY	1 cut(s)	2869
Psp6I	CCWGG	4 cut(s)	984, 2128, 2634, 2763
PspFI	CCCAGC	1 cut(s)	1606
PspGI	CCWGG	4 cut(s)	984, 2128, 2634, 2763
PspN4I	GGNNCC	5 cut(s)	1059, 2426, 2640, 2682, 2836
PspPI	GGNCC	7 cut(s)	982, 1162, 1393, 2680, 2869, 2893, 3084
PspPPI	RGGWCCY	1 cut(s)	2869
PstI	CTGCAG	1 cut(s)	12
PstNI	CAGNNNCTG	1 cut(s)	338
PsuI	RGATCY	3 cut(s)	373, 1057, 1408
PsyI	GACNNNGTC	1 cut(s)	1584
PvuII	CAGCTG	1 cut(s)	1774
RsaI	GTAC	5 cut(s)	5, 22, 459, 2689, 2836
RsaNI	GTAC	5 cut(s)	4, 21, 458, 2688, 2835
RseI	CAYNNNNRTG	8 cut(s)	489, 660, 1658, 1736, 1782, 1824, 2375, 2975
SacI	GAGCTC	1 cut(s)	2085
SapI	GCTCTTC	1 cut(s)	2007
SaqAI	TTAA	9 cut(s)	1532, 1875, 1887, 2019, 2124, 2267, 2306, 2406, 2874
Sau96I	GGNCC	7 cut(s)	982, 1162, 1393, 2680, 2869, 2893, 3084
ScaI	AGTACT	2 cut(s)	459, 2689
ScrFI	CCNGG	6 cut(s)	986, 2130, 2506, 2636, 2765, 2831
SduI	GDGCHC	7 cut(s)	430, 599, 926, 1762, 2085, 2372, 3133
SfaNI	GCATC	9 cut(s)	57, 1026, 1740, 1780, 1802, 1988, 2523, 3016, 3050
SfcI	CTRYAG	1 cut(s)	8
SfuI	TTCGAA	2 cut(s)	3103, 3201
SinI	GGWCC	4 cut(s)	1162, 1393, 2869, 2893
SmiMI	CAYNNNNRTG	8 cut(s)	489, 660, 1658, 1736, 1782, 1824, 2375, 2975
SmlI	CTYRAG	3 cut(s)	1281, 2670, 2691
SmoI	CTYRAG	3 cut(s)	1281, 2670, 2691
SpeI	ACTAGT	1 cut(s)	1472
SphI	GCATGC	1 cut(s)	522
SseBI	AGGCCT	1 cut(s)	38
SsiI	CCGC	8 cut(s)	121, 184, 329, 336, 432, 602, 803, 2559
SspI	AATATT	1 cut(s)	2851
SspMI	CTAG	8 cut(s)	515, 798, 1178, 1473, 2513, 2600, 2897, 3005
SstI	GAGCTC	1 cut(s)	2085
StuI	AGGCCT	1 cut(s)	38
StyD4I	CCNGG	6 cut(s)	984, 2128, 2504, 2634, 2763, 2829
StyI	CCWWGG	6 cut(s)	1437, 2184, 2663, 2821, 2838, 2952
TaaI	ACNGT	9 cut(s)	20, 91, 457, 662, 682, 1173, 1321, 1453, 2279
TaiI	ACGT	2 cut(s)	1588, 2522
TaqI	TCGA	6 cut(s)	991, 1017, 1260, 1631, 3103, 3201
TaqII	GACCGA	1 cut(s)	1930
TatI	WGTACW	2 cut(s)	457, 2687
TauI	GCSGC	3 cut(s)	123, 331, 338
TfiI	GAWTC	7 cut(s)	794, 1417, 1616, 2058, 2118, 2349, 3203
Tru1I	TTAA	9 cut(s)	1532, 1875, 1887, 2019, 2124, 2267, 2306, 2406, 2874
Tru9I	TTAA	9 cut(s)	1532, 1875, 1887, 2019, 2124, 2267, 2306, 2406, 2874
TscAI	CASTG	7 cut(s)	426, 1660, 1762, 1989, 2034, 2377, 3016
TseFI	GTSAC	6 cut(s)	1000, 1048, 1432, 1651, 2092, 2279
TseI	GCWGC	8 cut(s)	10, 287, 338, 1771, 1774, 2415, 2604, 3059
Tsp45I	GTSAC	6 cut(s)	1000, 1048, 1432, 1651, 2092, 2279
TspGWI	ACGGA	1 cut(s)	2719
TspRI	CASTG	7 cut(s)	426, 1660, 1762, 1989, 2034, 2377, 3016
Tth111I	GACNNNGTC	1 cut(s)	1584
VneI	GTGCAC	1 cut(s)	1758
VpaK11BI	GGWCC	4 cut(s)	1162, 1393, 2869, 2893
XapI	RAATTY	7 cut(s)	623, 764, 1266, 1857, 1952, 2481, 3112
XbaI	TCTAGA	1 cut(s)	797
XceI	RCATGY	3 cut(s)	522, 1134, 1813
XmnI	GAANNNNTTC	1 cut(s)	1256
XspI	CTAG	8 cut(s)	515, 798, 1178, 1473, 2513, 2600, 2897, 3005
ZrmI	AGTACT	2 cut(s)	459, 2689
Zsp2I	ATGCAT	2 cut(s)	1795, 2538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.