Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

70152906 .. 70156267

Gene Structure 3362 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020921

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGCCAAACAGTACCATTGGTGATCAAGCTTCTGAAGGTGCCAGCCACTCGACTTTTACTACTGACCTTATTCAAACCGTTAGTAGTTGTGTCCAATATGAGCAGAGCAAGCAGAGCCGCTGGTATAAATACATTGCTGTTGGGGTTTTCATCTGGCGCACCAGAACCAAGTCACGCCTCCGCAAAGCAAAGGCCCCTCGTTATCAACGGTTTCTGAAAAAGGTTGCTCGCCTTGATGTGAGACAGACGGTCTCACACCTCGGATCCTACTTCAAGGATCGTGCTGCTTATATCCTTTTATTAGCACCAACAACTCCAACAACACCGCCAGACGCTGCTGCCTCAAATCATGTGATTGACATCCCTCTTCAAGAAAGGCATCTGAAAGAGGTTGCTCGCATTGTTAGAGAGAAGGACTTGAATGCACTGCAAGCACACGGTGTTGTTGATGTTGTTTCAACACTTCTCAGGTCTCACTCTGAGGATCATGCTGATGATGGTGGTCAAGTTCCACAACTATCATTGAAGACTACCAAGCCTGTTTTCTTTCACTTCTTGTTGAAGGCTTGCAACCAGTACACAATCTTTTTCCTCCTAGTCTCTGCTGGGTTGCAATTTGCTATTGAATTTATGAAGCAAGGAGTCAAACAAGGGTGGCATGATGGTGTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTTCCTTCAGTTGGGAACTATCGCAACGAGTGGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTTCTGGTGAATGTTGAAAGAAATGGAGAATCTAGGGCGGTTATGATATCTCATGTTTCGGTGGGTGATATAGTTCATTTGAAGAAGGGAGACCATGCTCCTGCTGATGGCTTGTTCATAGATCATGGTGCGAAACTGGTCTTGGATGAGGTATTGAGCCCCACAATTGATTTTGAAAACAACCCATTTGTGTTGTCTGGTTCAAAAGTTACTGAGGGCCAGGGTCGAATGATTGTGACATCTGTTGGTGTCGATACAGCTTGTGGAGAGATGCTGAGCTTGGTGACTGAGGATCATAATCCAAATAGGAAGACTCTGTTACAAGCTTTGATGGACAAACCATATACTTATATGGAGTATCTTGCGTGTTGTGTCGCGGTGCTAATTGCTGTCGTGGTGCTAATACGGTTAGTACTTTTCAGGAAGCATGACAAGTACAATGACAGGCCAGACCTGAAAGGGGAGGTTTCAATGAACTTGCTGATGAAGATTTTTGAGAAAATATGCTTGAAAACTCAAGGAACCGTCTCCACTTTGGTGAGTGTTCTTGCTACTGTGGTAATAGGGATACAACATGGAATGCCCTTTGTGATTTCGCTTGCAATTTGTCAATGGAATAAGAAGGTGGTCCAAAATCGGGCAGATCCTCAGAATCTTTCAGCTTGTGTCACCATGGGCCTCGTAACGGTCATCTGCATTGAAACAACTAGTGAGAAAATGTGCAACGAGATGGAGGTGATGGACTTTTGGATAGGTGATAAGGATTTAAGCAAGGAAGTGGAGTCTGATCAAACTGAACAATTTGCTCTAAAAGGACTACGTCAGGAGATTTCTGCTACCCCAGCAAATGATTTGTTCATTTCTACGATCAAGACCAGATGGGGTGTTACTGACATGGAGGTATTGGATCAGACATTAATAACTCTGGAAGAGAGAAAATTGAGTTCTGGCATCTTAATGAGGAAAACAGAGAGCAGTGCGCAAGATATGCAGCTGCATATGAGTGGAGATGCATCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACTGGGAAAAGTCGTATTCTGGAAATTCAGAAGAGAAAGTTTAAGCAGGTCATTAAGAACATGGAGGAGAAGGGTCTTAGACCGATTGCATATGCTTACAAGAAAACAAATGAACAAGAATTTACAGAAAATGGGTTGAAATTGTTGGCACTGGTGGGAGTCAGATGCCCATATCAAGAAGAGCTTAAAATTGCAGTGGAAGCTTTTAGAAAGGCCGGAATAAGAATCAAGCTGGTATCCGAGGATGAGCTCTCAATAGTGAGTGAGCAAGCTTCTGATCTTGGAATCTTTAACCCTGGTAGTGGTGATATGGAGATCGCAGGCGAAACTTTCAGAAGACTCAACTCCATGGAAAGACTAAATAAGGTGGATTTGATATCCGTGATGGGAAGTTCCCTCCCAAAGGACAAGCTTCTCATGGTGGAGCGCCTAAAGAAAAACGGTCACATAGTGGCATTCTATGGAGGGCTAACTGTAAACGATTACCCGACTTTAAAAGAAGCTGATGTTGGAATCACAGAGGATATCAGGAGCACTGAAATGGCTAGAGACAATGCTGATATTATAGTAAAAGATGGCTGCTTATGGTTCCTCAATCTGAAGTCTGGGAAATGCGCTTACCACAACATTCAGAACTTCTGTCAAATTCAGCTGACAGCTTGCATATCCGGGTTGCTAGTGACGTTAGTAGCAACGATGCATTCAGGGGACTCTCCATTGACCGCAACTCACTTGATTTGGGTGAACTTGATAATGTGCCTTCTAGGCGGCCTAATGATGGTAATGGAGTTAAAAGGCCAGGAGCTACTTGCTCGAAAACCGATGCTCAGGACTCAGCCACTTATAACCAAAGCCATCTGGAGAAACATAGCCATTCAAGTTTCATACCAGGCTTCCATCTTGCTGGTCTTGCATTTCATGGGAAATGCAATAGCGAGCATGAACCAAGGCATCCGGGGTACCATGGTTTTCAATATTTACACCTTGTGTCAGGTCCTTAATCTGTTCAGGGCCATGGACCTAGTGAACAAGGAAGTGCTAAATGTTGTTCTTCATAGCTATTGGTTTTTGATGGCCTTGGGAGCTGTTATGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGACTAGCCAGTGGTGTGAGGTTGGATGCAATACAGTGGGCATCCTGCTTCCTTCTTGCTGCTCTTTCATGGCCGTTTGATTGGGCCATAACTTCGCAGTTTCGAAGAGAAAATTTGGCATTGGGGAGAGCACCTATGGGGATGTCAAGATTTTACATTTGTGCTTCGTTGTTCCTCATTTTCTCTGTATCATATTGTTTCGAATCAGATTATGCACACACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1058

Amino Acids

118.44

Weight (kDa)

8.45

Isoelectric Point (pI)

33.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	259 - 351	6.2e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	577 - 666	2.4e-08	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	845 - 1011	2.7e-25	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1812, 2666
AarI	CACCTGC	1 cut(s)	2911
Acc16I	TGCGCA	1 cut(s)	1749
Acc36I	ACCTGC	2 cut(s)	1858, 2911
Acc65I	GGTACC	1 cut(s)	2780
AccB1I	GGYRCC	2 cut(s)	38, 2780
AccII	CGCG	1 cut(s)	1145
AciI	CCGC	7 cut(s)	118, 181, 326, 806, 1145, 2544, 2589
AclWI	GGATC	7 cut(s)	258, 271, 285, 492, 1068, 1406, 1683
AcoI	YGGCCR	1 cut(s)	3017
AcsI	RAATTY	6 cut(s)	626, 767, 1845, 1940, 2466, 3058
AcuI	CTGAAG	3 cut(s)	54, 693, 2441
AdeI	CACNNNGTG	3 cut(s)	440, 2272, 2808
AfaI	GTAC	5 cut(s)	13, 578, 1182, 1205, 2782
AfiI	CCNNNNNNNGG	8 cut(s)	713, 875, 1228, 1405, 1453, 2123, 2224, 2599
AflIII	ACRYGT	1 cut(s)	1797
AhdI	GACNNNNNGTC	1 cut(s)	248
AhlI	ACTAGT	1 cut(s)	1475
AjnI	CCWGG	4 cut(s)	987, 2116, 2619, 2709
AjuI	GAANNNNNNNTTGG	6 cut(s)	527, 559, 893, 925, 2313, 2345
AleI	CACNNNNGTG	1 cut(s)	1304
Alw21I	GWGCWC	3 cut(s)	2073, 2357, 3079
Alw26I	GTCTC	7 cut(s)	235, 256, 477, 604, 852, 1300, 2364
AlwI	GGATC	7 cut(s)	258, 271, 285, 492, 1068, 1406, 1683
AlwNI	CAGNNNCTG	1 cut(s)	335
ApeKI	GCWGC	7 cut(s)	284, 335, 338, 1759, 1762, 2400, 3005
ApoI	RAATTY	6 cut(s)	626, 767, 1845, 1940, 2466, 3058
AseI	ATTAAT	1 cut(s)	1685
Asp718I	GGTACC	1 cut(s)	2780
AspLEI	GCGC	4 cut(s)	159, 1750, 2250, 2438
AspS9I	GGNCC	8 cut(s)	193, 985, 1396, 1444, 2815, 2832, 2839, 3030
AsuC2I	CCSGG	2 cut(s)	2491, 2777
AsuII	TTCGAA	2 cut(s)	3049, 3147
AvaII	GGWCC	3 cut(s)	1396, 2815, 2839
BaeI	ACNNNNGTAYC	2 cut(s)	2772, 2805
BamHI	GGATCC	1 cut(s)	263
BanI	GGYRCC	2 cut(s)	38, 2780
BanII	GRGCYC	2 cut(s)	929, 2073
BbsI	GAAGAC	3 cut(s)	533, 1085, 2164
Bbv12I	GWGCWC	3 cut(s)	2073, 2357, 3079
BbvI	GCAGC	7 cut(s)	271, 322, 325, 1749, 1771, 2387, 2992
BceAI	ACGGC	1 cut(s)	3004
BciT130I	CCWGG	4 cut(s)	989, 2118, 2621, 2711
BciVI	GTATCC	2 cut(s)	1329, 2068
BclI	TGATCA	2 cut(s)	22, 1555
BcnI	CCSGG	2 cut(s)	2491, 2777
BcoDI	GTCTC	7 cut(s)	235, 256, 477, 604, 852, 1300, 2364
BcuI	ACTAGT	1 cut(s)	1475
BfaI	CTAG	8 cut(s)	596, 801, 1476, 2367, 2498, 2585, 2843, 2951
BfoI	RGCGCY	1 cut(s)	2251
BfuAI	ACCTGC	2 cut(s)	1858, 2911
BfuI	GTATCC	2 cut(s)	1329, 2068
BglI	GCCNNNNNGGC	1 cut(s)	2586
BisI	GCNGC	9 cut(s)	118, 285, 336, 339, 1760, 1763, 2401, 2590, 3006
BlpI	GCTNAGC	1 cut(s)	1043
BlsI	GCNGC	9 cut(s)	119, 286, 337, 340, 1761, 1764, 2402, 2591, 3007
BmcAI	AGTACT	1 cut(s)	1182
Bme1390I	CCNGG	6 cut(s)	989, 2118, 2491, 2621, 2711, 2777
Bme18I	GGWCC	3 cut(s)	1396, 2815, 2839
BmeRI	GACNNNNNGTC	1 cut(s)	248
BmgT120I	GGNCC	8 cut(s)	193, 985, 1396, 1444, 2815, 2832, 2839, 3030
BmiI	GGNNCC	6 cut(s)	40, 195, 265, 1291, 2411, 2782
BmrFI	CCNGG	6 cut(s)	989, 2118, 2491, 2621, 2711, 2777
BmrI	ACTGGG	1 cut(s)	1833
BmuI	ACTGGG	1 cut(s)	1833
BpiI	GAAGAC	3 cut(s)	533, 1085, 2164
BpmI	CTGGAG	1 cut(s)	2701
Bpu10I	CCTNAGC	1 cut(s)	2648
Bpu1102I	GCTNAGC	1 cut(s)	1043
Bpu14I	TTCGAA	2 cut(s)	3049, 3147
BpuEI	CTTGAG	1 cut(s)	1269
BpuMI	CCSGG	2 cut(s)	2491, 2777
BsaBI	GATNNNNATC	2 cut(s)	359, 1065
BsaI	GGTCTC	3 cut(s)	256, 477, 852
Bsc4I	CCNNNNNNNGG	8 cut(s)	713, 875, 1228, 1405, 1453, 2123, 2224, 2599
Bse1I	ACTGG	5 cut(s)	574, 909, 1828, 1977, 2955
Bse3DI	GCAATG	1 cut(s)	132
Bse8I	GATNNNNATC	2 cut(s)	359, 1065
BseBI	CCWGG	4 cut(s)	989, 2118, 2621, 2711
BseGI	GGATG	7 cut(s)	360, 919, 2071, 2772, 2977, 2987, 3093
BseJI	GATNNNNATC	2 cut(s)	359, 1065
BseLI	CCNNNNNNNGG	8 cut(s)	713, 875, 1228, 1405, 1453, 2123, 2224, 2599
BseMI	GCAATG	1 cut(s)	132
BseMII	CTCAG	8 cut(s)	471, 481, 972, 1034, 1047, 1430, 2662, 2669
BseNI	ACTGG	5 cut(s)	574, 909, 1828, 1977, 2955
BseRI	GAGGAG	1 cut(s)	1901
BseXI	GCAGC	7 cut(s)	271, 322, 325, 1749, 1771, 2387, 2992
BseYI	CCCAGC	2 cut(s)	605, 1609
Bsh1236I	CGCG	1 cut(s)	1145
BshNI	GGYRCC	2 cut(s)	38, 2780
BsiHKAI	GWGCWC	3 cut(s)	2073, 2357, 3079
BsiSI	CCGG	3 cut(s)	2037, 2490, 2776
BslFI	GGGAC	1 cut(s)	2543
BslI	CCNNNNNNNGG	8 cut(s)	713, 875, 1228, 1405, 1453, 2123, 2224, 2599
BsmAI	GTCTC	7 cut(s)	235, 256, 477, 604, 852, 1300, 2364
BsmBI	CGTCTC	1 cut(s)	1300
BsmFI	GGGAC	1 cut(s)	2543
BsmI	GAATGC	4 cut(s)	427, 1353, 2276, 2521
Bso31I	GGTCTC	3 cut(s)	256, 477, 852
Bsp119I	TTCGAA	2 cut(s)	3049, 3147
Bsp1286I	GDGCHC	4 cut(s)	929, 2073, 2357, 3079
Bsp1720I	GCTNAGC	1 cut(s)	1043
Bsp19I	CCATGG	4 cut(s)	1440, 2169, 2784, 2835
BspACI	CCGC	7 cut(s)	118, 181, 326, 806, 1145, 2544, 2589
BspCNI	CTCAG	8 cut(s)	472, 480, 973, 1035, 1048, 1429, 2661, 2668
BspFNI	CGCG	1 cut(s)	1145
BspLI	GGNNCC	6 cut(s)	40, 195, 265, 1291, 2411, 2782
BspMI	ACCTGC	2 cut(s)	1858, 2911
BspPI	GGATC	7 cut(s)	258, 271, 285, 492, 1068, 1406, 1683
BspQI	GCTCTTC	1 cut(s)	1995
BspT104I	TTCGAA	2 cut(s)	3049, 3147
BspT107I	GGYRCC	2 cut(s)	38, 2780
BspTNI	GGTCTC	3 cut(s)	256, 477, 852
BsrDI	GCAATG	1 cut(s)	132
BsrI	ACTGG	5 cut(s)	574, 909, 1828, 1977, 2955
BssT1I	CCWWGG	6 cut(s)	1440, 2169, 2767, 2784, 2835, 2898
Bst2UI	CCWGG	4 cut(s)	989, 2118, 2621, 2711
Bst6I	CTCTTC	5 cut(s)	372, 1692, 1847, 1995, 3046
BstAPI	GCANNNNNTGC	1 cut(s)	1756
BstBI	TTCGAA	2 cut(s)	3049, 3147
BstDSI	CCRYGG	4 cut(s)	1440, 2169, 2784, 2835
BstENI	CCTNNNNNAGG	1 cut(s)	2222
BstF5I	GGATG	7 cut(s)	360, 919, 2071, 2772, 2977, 2987, 3093
BstFNI	CGCG	1 cut(s)	1145
BstH2I	RGCGCY	1 cut(s)	2251
BstHHI	GCGC	4 cut(s)	159, 1750, 2250, 2438
BstMAI	GTCTC	7 cut(s)	235, 256, 477, 604, 852, 1300, 2364
BstMWI	GCNNNNNNNGC	9 cut(s)	109, 114, 431, 1610, 1756, 2021, 2586, 2731, 2903
BstNI	CCWGG	4 cut(s)	989, 2118, 2621, 2711
BstNSI	RCATGY	1 cut(s)	1801
BstSCI	CCNGG	6 cut(s)	987, 2116, 2489, 2619, 2709, 2775
BstUI	CGCG	1 cut(s)	1145
BstV1I	GCAGC	7 cut(s)	271, 322, 325, 1749, 1771, 2387, 2992
BstV2I	GAAGAC	3 cut(s)	533, 1085, 2164
BstX2I	RGATCY	2 cut(s)	263, 1411
BstXI	CCANNNNNNTGG	1 cut(s)	2725
BstYI	RGATCY	2 cut(s)	263, 1411
BsuI	GTATCC	2 cut(s)	1329, 2068
BtgI	CCRYGG	4 cut(s)	1440, 2169, 2784, 2835
BtsCI	GGATG	7 cut(s)	360, 919, 2071, 2772, 2977, 2987, 3093
BtsI	GCAGTG	3 cut(s)	425, 1750, 2022
BtsIMutI	CAGTG	7 cut(s)	425, 1750, 1970, 2022, 2355, 2962, 2987
BveI	ACCTGC	2 cut(s)	1858, 2911
CaiI	CAGNNNCTG	1 cut(s)	335
CfoI	GCGC	4 cut(s)	159, 1750, 2250, 2438
Cfr13I	GGNCC	8 cut(s)	193, 985, 1396, 1444, 2815, 2832, 2839, 3030
CseI	GACGC	1 cut(s)	341
Csp6I	GTAC	5 cut(s)	12, 577, 1181, 1204, 2781
CviQI	GTAC	5 cut(s)	12, 577, 1181, 1204, 2781
DraI	TTTAAA	1 cut(s)	2316
DraIII	CACNNNGTG	3 cut(s)	440, 2272, 2808
DriI	GACNNNNNGTC	1 cut(s)	248
EaeI	YGGCCR	1 cut(s)	3017
Eam1104I	CTCTTC	5 cut(s)	372, 1692, 1847, 1995, 3046
Eam1105I	GACNNNNNGTC	1 cut(s)	248
EarI	CTCTTC	5 cut(s)	372, 1692, 1847, 1995, 3046
Ecl136II	GAGCTC	1 cut(s)	2071
Eco130I	CCWWGG	6 cut(s)	1440, 2169, 2767, 2784, 2835, 2898
Eco24I	GRGCYC	2 cut(s)	929, 2073
Eco31I	GGTCTC	3 cut(s)	256, 477, 852
Eco32I	GATATC	3 cut(s)	816, 2199, 2347
Eco47I	GGWCC	3 cut(s)	1396, 2815, 2839
Eco53kI	GAGCTC	1 cut(s)	2071
Eco57I	CTGAAG	3 cut(s)	54, 693, 2441
EcoICRI	GAGCTC	1 cut(s)	2071
EcoNI	CCTNNNNNAGG	1 cut(s)	2222
EcoO109I	RGGNCCY	2 cut(s)	193, 2815
EcoRII	CCWGG	4 cut(s)	987, 2116, 2619, 2709
EcoRV	GATATC	3 cut(s)	816, 2199, 2347
EcoT14I	CCWWGG	6 cut(s)	1440, 2169, 2767, 2784, 2835, 2898
EcoT22I	ATGCAT	2 cut(s)	1783, 2523
EcoT38I	GRGCYC	2 cut(s)	929, 2073
ErhI	CCWWGG	6 cut(s)	1440, 2169, 2767, 2784, 2835, 2898
Esp3I	CGTCTC	1 cut(s)	1300
FalI	AAGNNNNNCTT	2 cut(s)	1989, 2021
FaqI	GGGAC	1 cut(s)	2543
FauNDI	CATATG	2 cut(s)	1767, 1912
FbaI	TGATCA	2 cut(s)	22, 1555
Fnu4HI	GCNGC	9 cut(s)	118, 285, 336, 339, 1760, 1763, 2401, 2590, 3006
FokI	GGATG	7 cut(s)	347, 926, 2078, 2759, 2974, 2984, 3100
FriOI	GRGCYC	2 cut(s)	929, 2073
Fsp4HI	GCNGC	9 cut(s)	118, 285, 336, 339, 1760, 1763, 2401, 2590, 3006
FspBI	CTAG	8 cut(s)	596, 801, 1476, 2367, 2498, 2585, 2843, 2951
FspI	TGCGCA	1 cut(s)	1749
GlaI	GCGC	4 cut(s)	158, 1749, 2249, 2437
GluI	GCNGC	9 cut(s)	118, 285, 336, 339, 1760, 1763, 2401, 2590, 3006
GsaI	CCCAGC	2 cut(s)	609, 1613
GsuI	CTGGAG	1 cut(s)	2701
HaeII	RGCGCY	1 cut(s)	2251
HapII	CCGG	3 cut(s)	2037, 2490, 2776
HgaI	GACGC	1 cut(s)	341
HhaI	GCGC	4 cut(s)	159, 1750, 2250, 2438
Hin6I	GCGC	4 cut(s)	157, 1748, 2248, 2436
HinP1I	GCGC	4 cut(s)	157, 1748, 2248, 2436
HindIII	AAGCTT	5 cut(s)	27, 1092, 2022, 2091, 2231
HpaII	CCGG	3 cut(s)	2037, 2490, 2776
Hpy166II	GTNNAC	5 cut(s)	579, 1805, 2299, 2566, 2848
Hpy8I	GTNNAC	5 cut(s)	579, 1805, 2299, 2566, 2848
HpyAV	CCTTC	9 cut(s)	29, 406, 556, 717, 847, 1384, 1885, 2591, 3008
HpyCH4IV	ACGT	2 cut(s)	1588, 2504
HpyF10VI	GCNNNNNNNGC	9 cut(s)	109, 114, 431, 1610, 1756, 2021, 2586, 2731, 2903
HpySE526I	ACGT	2 cut(s)	1588, 2504
HspAI	GCGC	4 cut(s)	157, 1748, 2248, 2436
KpnI	GGTACC	1 cut(s)	2784
Ksp22I	TGATCA	2 cut(s)	22, 1555
LguI	GCTCTTC	1 cut(s)	1995
LmnI	GCTCC	5 cut(s)	871, 2245, 2352, 2623, 2903
Lsp1109I	GCAGC	7 cut(s)	271, 322, 325, 1749, 1771, 2387, 2992
MaeI	CTAG	8 cut(s)	596, 801, 1476, 2367, 2498, 2585, 2843, 2951
MaeII	ACGT	2 cut(s)	1588, 2504
MfeI	CAATTG	1 cut(s)	933
MflI	RGATCY	2 cut(s)	263, 1411
MhlI	GDGCHC	4 cut(s)	929, 2073, 2357, 3079
MlyI	GAGTC	7 cut(s)	651, 1075, 1559, 1989, 2154, 2525, 2647
MmeI	TCCRAC	3 cut(s)	341, 2311, 2949
Mph1103I	ATGCAT	2 cut(s)	1783, 2523
MslI	CAYNNNNRTG	8 cut(s)	492, 663, 1304, 1724, 1770, 1812, 2360, 2921
MspA1I	CMGCKG	3 cut(s)	120, 1762, 2473
MspI	CCGG	3 cut(s)	2037, 2490, 2776
MspR9I	CCNGG	6 cut(s)	989, 2118, 2491, 2621, 2711, 2777
MunI	CAATTG	1 cut(s)	933
Mva1269I	GAATGC	4 cut(s)	427, 1353, 2276, 2521
MvaI	CCWGG	4 cut(s)	989, 2118, 2621, 2711
MvnI	CGCG	1 cut(s)	1145
MwoI	GCNNNNNNNGC	9 cut(s)	109, 114, 431, 1610, 1756, 2021, 2586, 2731, 2903
NciI	CCSGG	2 cut(s)	2491, 2777
NcoI	CCATGG	4 cut(s)	1440, 2169, 2784, 2835
NdeI	CATATG	2 cut(s)	1767, 1912
NlaIV	GGNNCC	6 cut(s)	40, 195, 265, 1291, 2411, 2782
NmuCI	GTSAC	6 cut(s)	171, 1003, 1051, 1435, 2264, 2500
NsbI	TGCGCA	1 cut(s)	1749
NsiI	ATGCAT	2 cut(s)	1783, 2523
NspI	RCATGY	1 cut(s)	1801
NspV	TTCGAA	2 cut(s)	3049, 3147
OliI	CACNNNNGTG	1 cut(s)	1304
PaqCI	CACCTGC	1 cut(s)	2911
PciI	ACATGT	1 cut(s)	1797
PciSI	GCTCTTC	1 cut(s)	1995
PctI	GAATGC	4 cut(s)	427, 1353, 2276, 2521
PfeI	GAWTC	6 cut(s)	797, 1420, 2046, 2106, 2334, 3149
PflFI	GACNNNGTC	1 cut(s)	1587
PkrI	GCNGC	9 cut(s)	119, 286, 337, 340, 1761, 1764, 2402, 2591, 3007
PleI	GAGTC	7 cut(s)	650, 1075, 1558, 1988, 2154, 2525, 2647
PpsI	GAGTC	7 cut(s)	650, 1075, 1558, 1988, 2154, 2525, 2647
PpuMI	RGGWCCY	1 cut(s)	2815
PscI	ACATGT	1 cut(s)	1797
PshBI	ATTAAT	1 cut(s)	1685
PsiI	TTATAA	2 cut(s)	1812, 2666
Psp124BI	GAGCTC	1 cut(s)	2073
Psp5II	RGGWCCY	1 cut(s)	2815
Psp6I	CCWGG	4 cut(s)	987, 2116, 2619, 2709
PspFI	CCCAGC	2 cut(s)	605, 1609
PspGI	CCWGG	4 cut(s)	987, 2116, 2619, 2709
PspN4I	GGNNCC	6 cut(s)	40, 195, 265, 1291, 2411, 2782
PspPI	GGNCC	8 cut(s)	193, 985, 1396, 1444, 2815, 2832, 2839, 3030
PspPPI	RGGWCCY	1 cut(s)	2815
PstNI	CAGNNNCTG	1 cut(s)	335
PsuI	RGATCY	2 cut(s)	263, 1411
PsyI	GACNNNGTC	1 cut(s)	1587
PvuII	CAGCTG	2 cut(s)	1762, 2473
RsaI	GTAC	5 cut(s)	13, 578, 1182, 1205, 2782
RsaNI	GTAC	5 cut(s)	12, 577, 1181, 1204, 2781
RseI	CAYNNNNRTG	8 cut(s)	492, 663, 1304, 1724, 1770, 1812, 2360, 2921
SacI	GAGCTC	1 cut(s)	2073
SapI	GCTCTTC	1 cut(s)	1995
SatI	GCNGC	9 cut(s)	118, 285, 336, 339, 1760, 1763, 2401, 2590, 3006
Sau96I	GGNCC	8 cut(s)	193, 985, 1396, 1444, 2815, 2832, 2839, 3030
ScaI	AGTACT	1 cut(s)	1182
SchI	GAGTC	7 cut(s)	651, 1075, 1559, 1989, 2154, 2525, 2647
ScrFI	CCNGG	6 cut(s)	989, 2118, 2491, 2621, 2711, 2777
SduI	GDGCHC	4 cut(s)	929, 2073, 2357, 3079
SfuI	TTCGAA	2 cut(s)	3049, 3147
SinI	GGWCC	3 cut(s)	1396, 2815, 2839
SmiMI	CAYNNNNRTG	8 cut(s)	492, 663, 1304, 1724, 1770, 1812, 2360, 2921
SmlI	CTYRAG	1 cut(s)	1284
SmoI	CTYRAG	1 cut(s)	1284
SpeI	ACTAGT	1 cut(s)	1475
SsiI	CCGC	7 cut(s)	118, 181, 326, 806, 1145, 2544, 2589
SspI	AATATT	1 cut(s)	2797
SspMI	CTAG	8 cut(s)	596, 801, 1476, 2367, 2498, 2585, 2843, 2951
SstI	GAGCTC	1 cut(s)	2073
StyD4I	CCNGG	6 cut(s)	987, 2116, 2489, 2619, 2709, 2775
StyI	CCWWGG	6 cut(s)	1440, 2169, 2767, 2784, 2835, 2898
TaiI	ACGT	2 cut(s)	1591, 2507
TaqI	TCGA	6 cut(s)	50, 994, 1020, 2635, 3049, 3147
TaqII	GACCGA	1 cut(s)	1918
TatI	WGTACW	3 cut(s)	576, 1180, 1203
TauI	GCSGC	2 cut(s)	120, 2592
TfiI	GAWTC	6 cut(s)	797, 1420, 2046, 2106, 2334, 3149
TscAI	CASTG	7 cut(s)	432, 1750, 1977, 2022, 2362, 2962, 2987
TseFI	GTSAC	6 cut(s)	171, 1003, 1051, 1435, 2264, 2500
TseI	GCWGC	7 cut(s)	284, 335, 338, 1759, 1762, 2400, 3005
Tsp45I	GTSAC	6 cut(s)	171, 1003, 1051, 1435, 2264, 2500
TspGWI	ACGGA	1 cut(s)	2191
TspRI	CASTG	7 cut(s)	432, 1750, 1977, 2022, 2362, 2962, 2987
Tth111I	GACNNNGTC	1 cut(s)	1587
VpaK11BI	GGWCC	3 cut(s)	1396, 2815, 2839
VspI	ATTAAT	1 cut(s)	1685
XagI	CCTNNNNNAGG	1 cut(s)	2222
XapI	RAATTY	6 cut(s)	626, 767, 1845, 1940, 2466, 3058
XceI	RCATGY	1 cut(s)	1801
XspI	CTAG	8 cut(s)	596, 801, 1476, 2367, 2498, 2585, 2843, 2951
ZrmI	AGTACT	1 cut(s)	1182
Zsp2I	ATGCAT	2 cut(s)	1783, 2523

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.