Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

74229793 .. 74233810

Gene Structure 4018 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG533100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3123 bp

ATGCCAAACAGTACCAATGGTGATGAGGCCTCTGAAGGCATCAGCAACCCGACTCTTACTACTGAACTTAAGCAGAGCCGCTGGTATAGATACTATGTTATTGTGGTTTTCATCTGGCGCTCCGGAACCAAGCCACGCCTCCGCAGAGCAAAGGCCCCTTATCATCAACGGTTTCTGAAAAAGTTTGCTCGCCTTGATGTGAGACAGGACCTCAGATCCTACTTCAAGGATCGTGCTGCCTATATACCTTTGTTAGCGCCAACAACACCAACAACGCCGCCAGCCGCTGCCTCAAATCATGAGATTGACGAAGTTGCTCGCATTGTGAGAGAGAAGGACTTGAATGCACTGCGAGCACGCGGTGGTGTTGATGCTGTTTCAACACCTCTCAAGTCTCACTCTGAGAATCATGCTGACGATGGTGGTCAAGTTCCACAACTATCATTGAAGACTACCAAGCCTGTTTTCTTTCACTTCTTGTTGAAGGCTTGCAATCAGTACACAATCTTTTTCCTCCTAGGCTCAGCTGGGTTGCAATTTGCTATTGAATTTATGGAGCAAGGAGTCAAACAAGGGTGGCATGACGGTGTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTCCCTTCAGTTAGGAACTATCGCAACGAGTGGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTTCGGGTGAATGTTGAAAGAAATGGAGAATCTAGGGCGGTTATGATATCTCATGTTTCGGTGGGTGATATAGTTCATTTGAAGGAGGGAGACCATGCTCCTGCTGATGGCTTGTTCATAGATCATGGTGCGAAACTGGTCTTGGATGAGGTATTGAGCCCCACAATTGATTTTGAAAACAACCCATTTGTGTTGTCTGGTTCAAAAGTTATTGAGGGCCAGGGTCGAATGATTGTGGCATCTGTTGGTGTCGATACAGCTTGTGGAGAGATGCTGAGCTTGGTGACTGAGGGTCATAATCCAAATAGGAAGACTCTGTTACAAGCTTTGATGGACGAACCATATACTTATATGGAGTATCTTGCGTGTTGTGTCTCGGTGCTAATTGCTGTCGTGGTGCTAATACGGTTAGTACTTTTCAGGAAGCATGACAAGTACAATGACAGGCCAGACCTGAAAGGGGAGGTTTCAATGAACTTGCTGATGAAGATTTTTGAGAAAATATACTTGAAAACTCAAGGAACCGTCTCCACTTTGGTGAGTGTTCTTGCTACTGCGGTAATAGGGATACAACATGGAATGCCCTTTGTGATTTCGCTTGCAATTTGTCAATGGAATAAGAAGGTGGTCCAAAATCGGGCAGATCCTCAGAATCTTTCAGCTTGTGTCACCATGGGCCTCGTAACGGTCATCTGCATTGAAACAACTGGTGAGAAAATTCGTAACAAGATGGAGGTTATGGACTTTTGGATACGTGAAAAGGATTTAACCAATGAAGTGGAGTCTGATCAAATTGAACAATTTGCTCTGAGAGGACTACATCAAGAGGTTTCTGCTACCTCAGAAAATAATTTGTTCATTTATTTGATCAAGAACAGATGGGGTGTCACTGACATGGAGGTATTGGATCGCACATTTATAACTCTGGAAGAGAGAAAATTGAGTTCTGATGAGAAATGTAGTGGCATCTTAGTGAGGAAAACAGAGAGCAGCGCAGAAGATATGCAGCTGCATATAAATGGAGATGCGTCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACTGGGGAAAGTTCTATTATGGAAATTCAGAAGAGAAAGCTTAAGCAGGTCATTAAGAACATGGAGGAGAAGGGTCGAAGACCAATTGCATATGCTTATAAGAAAACAAATGTGAAAGAGTTTACAGAAAATGGGTTGACATTGTTGGCGCTAGTGGGAGTCAGACGCCCATATAAAGAAGAGCTTAAAATTGCAGTGGAAGCTTTCAGAAATGCTGGAAAAGAAATCAAGCTGGTTTCCGAGGATGAGCCCTCAATAGTGACTGCGCGAGCTTCTGATCTTGGAATCTTTAACCCTGGTATGGAAATTGCGGGCGAAGCTTTCAGAAGACTCAACTCCATGGAAAGACTAAATAAGGTGGATTTGATATCCGTGATGGGAAGTGCCCTTCCAAAGGACAAGCTTCTCATGGTGGAGCACTTAAAGAAAAAGGGTCACATAGTAGCGTTCTATGGAGGGCTAACCATAAAAGACACTCGTACTTTGATAGAAGCAGATATTGGAATCACAGAGGATATATGGAGCACTGAAATGGCCAGAGACAATGCTGATATTATAGTTAAGGATGGCTGCTTATGGTTCCCGAACTTGAAGTCTGGGAAATGTGCTTACCACAACATTCAGAACTTCTGTCAAATTCAGCTCACGATGATCATATCCGGGTTGCTAGTAACGTTAGTAGCAACGATGCATTCAGGGGACTCTCCATTGACTGCAATTCACTTGATTTGGGTGAACTTGATAATGTGCCTTCTAGGCAGCCAGATGATGGTAATGGAGTTAGAACGCCAGGAGCCACTTGCTCAAGAACCAGCCCCAAGGACTCAAGAACCGGCGCCAAGGACTCAGCCACTTATAACCCAAGCCATCTGGGGAAACATAGCCATTCAAGTTTCATACCAGGCTTCCACCTTGCTGGTCTTGCATTTCATGGGAAATGCAATAGCGAGCATGAACCAAGGCATCCGGGGTACCATGGTTTTCAATATTTACACCTTGTGTCAGGTCCTTAATCTGTTCAGGGCCATGGACCTAGTGAACAAGGAAGTCCTAAATGTTGTTCTTCACAGTTATTGCTTTTTGATGGCCTTGGGGGCTGTTATGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGACTAGCCAGTGGTGTGAGGTTGGATGCAATACAGTGGGCATCCTGCTTCCTTCTTGCTGCTCTTTCATGGCCGTTTGATTGGGCCATAACTTCGCAGTTTCGAAGAGAAAATTTGGCATTGAGGAGAGCACCTATGGGGATGTCAAGATTTTACATTTGTGCTTCGTTGTTCCTCATTTTCTCTGTATCATATTGTTTCGAATCAGATTATGCACACACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1040

Amino Acids

116.98

Weight (kDa)

7.98

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	232 - 325	2e-09	P-type ATPase actuator domain
Cation_ATPase	PF13246	524 - 647	8.7e-11	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	821 - 994	1e-19	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1610, 1749, 1857, 2615
AarI	CACCTGC	1 cut(s)	2860
Acc36I	ACCTGC	2 cut(s)	1795, 2860
Acc65I	GGTACC	1 cut(s)	2729
AccB1I	GGYRCC	2 cut(s)	2593, 2729
AccB7I	CCANNNNNTGG	1 cut(s)	2527
AccII	CGCG	2 cut(s)	360, 2026
AccIII	TCCGGA	1 cut(s)	122
AciI	CCGC	8 cut(s)	79, 142, 278, 285, 360, 728, 1247, 2069
AclI	AACGTT	1 cut(s)	2432
AclWI	GGATC	4 cut(s)	210, 237, 1328, 1605
AcoI	YGGCCR	2 cut(s)	2292, 2966
AcsI	RAATTY	6 cut(s)	548, 689, 1407, 1782, 2394, 3007
AcuI	CTGAAG	2 cut(s)	54, 615
AcyI	GRCGYC	2 cut(s)	1924, 2594
AdeI	CACNNNGTG	2 cut(s)	362, 2757
AfaI	GTAC	6 cut(s)	13, 500, 1104, 1127, 2239, 2731
AfiI	CCNNNNNNNGG	7 cut(s)	797, 1150, 1327, 1375, 2059, 2152, 2527
AflII	CTTAAG	2 cut(s)	68, 1799
AflIII	ACRYGT	1 cut(s)	1734
AhdI	GACNNNNNGTC	1 cut(s)	981
AjnI	CCWGG	4 cut(s)	909, 2053, 2547, 2658
AjuI	GAANNNNNNNTTGG	6 cut(s)	449, 481, 815, 847, 2241, 2273
AleI	CACNNNNGTG	1 cut(s)	1226
Alw21I	GWGCWC	4 cut(s)	358, 2178, 2285, 3028
Alw26I	GTCTC	6 cut(s)	196, 399, 774, 1069, 1222, 2292
AlwI	GGATC	4 cut(s)	210, 237, 1328, 1605
AlwNI	CAGNNNCTG	1 cut(s)	287
Aor13HI	TCCGGA	1 cut(s)	122
ApeKI	GCWGC	8 cut(s)	236, 287, 1680, 1696, 1699, 2328, 2517, 2954
ApoI	RAATTY	6 cut(s)	548, 689, 1407, 1782, 2394, 3007
ArsI	GACNNNNNNTTYG	2 cut(s)	2336, 2368
Asp718I	GGTACC	1 cut(s)	2729
AspA2I	CCTAGG	1 cut(s)	517
AspLEI	GCGC	6 cut(s)	120, 259, 1685, 1909, 2026, 2596
AspS9I	GGNCC	9 cut(s)	154, 208, 907, 1318, 1366, 2764, 2781, 2788, 2979
AsuC2I	CCSGG	2 cut(s)	2419, 2726
AsuII	TTCGAA	2 cut(s)	2998, 3096
AvaII	GGWCC	4 cut(s)	208, 1318, 2764, 2788
AvrII	CCTAGG	1 cut(s)	517
BaeGI	GKGCMC	1 cut(s)	2146
BaeI	ACNNNNGTAYC	2 cut(s)	2721, 2754
BalI	TGGCCA	1 cut(s)	2294
BanI	GGYRCC	2 cut(s)	2593, 2729
BanII	GRGCYC	2 cut(s)	851, 2010
BbsI	GAAGAC	4 cut(s)	455, 1007, 1843, 2092
Bbv12I	GWGCWC	4 cut(s)	358, 2178, 2285, 3028
BbvI	GCAGC	8 cut(s)	223, 274, 1686, 1692, 1708, 2315, 2529, 2941
BceAI	ACGGC	1 cut(s)	2953
BciT130I	CCWGG	4 cut(s)	911, 2055, 2549, 2660
BciVI	GTATCC	2 cut(s)	1251, 1434
BclI	TGATCA	3 cut(s)	1477, 1557, 2409
BcnI	CCSGG	2 cut(s)	2419, 2726
BcoDI	GTCTC	6 cut(s)	196, 399, 774, 1069, 1222, 2292
BfaI	CTAG	7 cut(s)	518, 723, 1910, 2426, 2513, 2792, 2900
BfoI	RGCGCY	4 cut(s)	121, 260, 1910, 2597
BfrI	CTTAAG	2 cut(s)	68, 1799
BfuAI	ACCTGC	2 cut(s)	1795, 2860
BfuI	GTATCC	2 cut(s)	1251, 1434
BglI	GCCNNNNNGGC	2 cut(s)	2514, 2852
BlnI	CCTAGG	1 cut(s)	517
BlpI	GCTNAGC	2 cut(s)	523, 965
BmcAI	AGTACT	1 cut(s)	1104
Bme1390I	CCNGG	6 cut(s)	911, 2055, 2419, 2549, 2660, 2726
Bme18I	GGWCC	4 cut(s)	208, 1318, 2764, 2788
BmeRI	GACNNNNNGTC	1 cut(s)	981
BmgT120I	GGNCC	9 cut(s)	154, 208, 907, 1318, 1366, 2764, 2781, 2788, 2979
BmiI	GGNNCC	7 cut(s)	127, 156, 1213, 2339, 2553, 2595, 2731
BmrFI	CCNGG	6 cut(s)	911, 2055, 2419, 2549, 2660, 2726
BmrI	ACTGGG	1 cut(s)	1770
BmuI	ACTGGG	1 cut(s)	1770
BpiI	GAAGAC	4 cut(s)	455, 1007, 1843, 2092
Bpu1102I	GCTNAGC	2 cut(s)	523, 965
Bpu14I	TTCGAA	2 cut(s)	2998, 3096
BpuEI	CTTGAG	4 cut(s)	374, 1191, 2547, 2568
BpuMI	CCSGG	2 cut(s)	2419, 2726
BsaAI	YACGTR	1 cut(s)	1445
BsaHI	GRCGYC	2 cut(s)	1924, 2594
BsaI	GGTCTC	1 cut(s)	774
BsaWI	WCCGGW	1 cut(s)	122
Bsc4I	CCNNNNNNNGG	7 cut(s)	797, 1150, 1327, 1375, 2059, 2152, 2527
Bse118I	RCCGGY	1 cut(s)	2590
Bse1I	ACTGG	4 cut(s)	831, 1402, 1765, 2904
BseAI	TCCGGA	1 cut(s)	122
BseBI	CCWGG	4 cut(s)	911, 2055, 2549, 2660
BseGI	GGATG	7 cut(s)	841, 2008, 2329, 2721, 2926, 2936, 3042
BseLI	CCNNNNNNNGG	7 cut(s)	797, 1150, 1327, 1375, 2059, 2152, 2527
BseMII	CTCAG	9 cut(s)	226, 393, 537, 956, 969, 1352, 1490, 1545, 2618
BseNI	ACTGG	4 cut(s)	831, 1402, 1765, 2904
BseRI	GAGGAG	2 cut(s)	1838, 3034
BseSI	GKGCMC	1 cut(s)	2146
BseXI	GCAGC	8 cut(s)	223, 274, 1686, 1692, 1708, 2315, 2529, 2941
BseYI	CCCAGC	1 cut(s)	527
Bsh1236I	CGCG	2 cut(s)	360, 2026
BshNI	GGYRCC	2 cut(s)	2593, 2729
BsiHKAI	GWGCWC	4 cut(s)	358, 2178, 2285, 3028
BsiSI	CCGG	4 cut(s)	123, 2418, 2591, 2725
BslFI	GGGAC	1 cut(s)	2471
BslI	CCNNNNNNNGG	7 cut(s)	797, 1150, 1327, 1375, 2059, 2152, 2527
BsmAI	GTCTC	6 cut(s)	196, 399, 774, 1069, 1222, 2292
BsmBI	CGTCTC	1 cut(s)	1222
BsmFI	GGGAC	1 cut(s)	2471
BsmI	GAATGC	3 cut(s)	349, 1275, 2449
Bso31I	GGTCTC	1 cut(s)	774
Bsp119I	TTCGAA	2 cut(s)	2998, 3096
Bsp1286I	GDGCHC	7 cut(s)	358, 851, 2010, 2146, 2178, 2285, 3028
Bsp13I	TCCGGA	1 cut(s)	122
Bsp143I	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
Bsp1720I	GCTNAGC	2 cut(s)	523, 965
Bsp19I	CCATGG	4 cut(s)	1362, 2097, 2733, 2784
BspACI	CCGC	8 cut(s)	79, 142, 278, 285, 360, 728, 1247, 2069
BspCNI	CTCAG	9 cut(s)	225, 394, 536, 957, 970, 1351, 1491, 1544, 2617
BspEI	TCCGGA	1 cut(s)	122
BspFNI	CGCG	2 cut(s)	360, 2026
BspHI	TCATGA	1 cut(s)	298
BspLI	GGNNCC	7 cut(s)	127, 156, 1213, 2339, 2553, 2595, 2731
BspMI	ACCTGC	2 cut(s)	1795, 2860
BspPI	GGATC	4 cut(s)	210, 237, 1328, 1605
BspQI	GCTCTTC	1 cut(s)	1932
BspT104I	TTCGAA	2 cut(s)	2998, 3096
BspT107I	GGYRCC	2 cut(s)	2593, 2729
BspTI	CTTAAG	2 cut(s)	68, 1799
BspTNI	GGTCTC	1 cut(s)	774
BsrFI	RCCGGY	1 cut(s)	2590
BsrI	ACTGG	4 cut(s)	831, 1402, 1765, 2904
BssAI	RCCGGY	1 cut(s)	2590
BssMI	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
BssNI	GRCGYC	2 cut(s)	1924, 2594
BssT1I	CCWWGG	9 cut(s)	517, 1362, 2097, 2576, 2597, 2716, 2733, 2784, 2847
Bst2UI	CCWGG	4 cut(s)	911, 2055, 2549, 2660
Bst4CI	ACNGT	9 cut(s)	11, 171, 587, 607, 1098, 1216, 1378, 2828, 2931
Bst6I	CTCTTC	4 cut(s)	1614, 1784, 1932, 2995
BstACI	GRCGYC	2 cut(s)	1924, 2594
BstAFI	CTTAAG	2 cut(s)	68, 1799
BstBAI	YACGTR	1 cut(s)	1445
BstBI	TTCGAA	2 cut(s)	2998, 3096
BstDSI	CCRYGG	4 cut(s)	1362, 2097, 2733, 2784
BstENI	CCTNNNNNAGG	1 cut(s)	2150
BstF5I	GGATG	7 cut(s)	841, 2008, 2329, 2721, 2926, 2936, 3042
BstFNI	CGCG	2 cut(s)	360, 2026
BstH2I	RGCGCY	4 cut(s)	121, 260, 1910, 2597
BstHHI	GCGC	6 cut(s)	120, 259, 1685, 1909, 2026, 2596
BstKTI	GATC	9 cut(s)	218, 232, 814, 1336, 1480, 1560, 1600, 2038, 2412
BstMAI	GTCTC	6 cut(s)	196, 399, 774, 1069, 1222, 2292
BstMBI	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
BstMWI	GCNNNNNNNGC	6 cut(s)	353, 1958, 2075, 2514, 2680, 2852
BstNI	CCWGG	4 cut(s)	911, 2055, 2549, 2660
BstNSI	RCATGY	1 cut(s)	1738
BstSCI	CCNGG	6 cut(s)	909, 2053, 2417, 2547, 2658, 2724
BstSLI	GKGCMC	1 cut(s)	2146
BstUI	CGCG	2 cut(s)	360, 2026
BstV1I	GCAGC	8 cut(s)	223, 274, 1686, 1692, 1708, 2315, 2529, 2941
BstV2I	GAAGAC	4 cut(s)	455, 1007, 1843, 2092
BstX2I	RGATCY	2 cut(s)	215, 1333
BstXI	CCANNNNNNTGG	2 cut(s)	1468, 2674
BstYI	RGATCY	2 cut(s)	215, 1333
BsuI	GTATCC	2 cut(s)	1251, 1434
BtgI	CCRYGG	4 cut(s)	1362, 2097, 2733, 2784
BtsCI	GGATG	7 cut(s)	841, 2008, 2329, 2721, 2926, 2936, 3042
BtsI	GCAGTG	2 cut(s)	347, 1959
BtsIMutI	CAGTG	6 cut(s)	347, 1578, 1959, 2283, 2911, 2936
BveI	ACCTGC	2 cut(s)	1795, 2860
CaiI	CAGNNNCTG	1 cut(s)	287
CciI	TCATGA	1 cut(s)	298
CfoI	GCGC	6 cut(s)	120, 259, 1685, 1909, 2026, 2596
Cfr10I	RCCGGY	1 cut(s)	2590
Cfr13I	GGNCC	9 cut(s)	154, 208, 907, 1318, 1366, 2764, 2781, 2788, 2979
CseI	GACGC	2 cut(s)	1707, 1932
Csp6I	GTAC	6 cut(s)	12, 499, 1103, 1126, 2238, 2730
CviQI	GTAC	6 cut(s)	12, 499, 1103, 1126, 2238, 2730
DinI	GGCGCC	1 cut(s)	2595
DpnI	GATC	9 cut(s)	217, 231, 813, 1335, 1479, 1559, 1599, 2037, 2411
DpnII	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
DraIII	CACNNNGTG	2 cut(s)	362, 2757
DriI	GACNNNNNGTC	1 cut(s)	981
EaeI	YGGCCR	2 cut(s)	2292, 2966
Eam1104I	CTCTTC	4 cut(s)	1614, 1784, 1932, 2995
Eam1105I	GACNNNNNGTC	1 cut(s)	981
EarI	CTCTTC	4 cut(s)	1614, 1784, 1932, 2995
Eco130I	CCWWGG	9 cut(s)	517, 1362, 2097, 2576, 2597, 2716, 2733, 2784, 2847
Eco147I	AGGCCT	1 cut(s)	29
Eco24I	GRGCYC	2 cut(s)	851, 2010
Eco31I	GGTCTC	1 cut(s)	774
Eco32I	GATATC	2 cut(s)	738, 2127
Eco47I	GGWCC	4 cut(s)	208, 1318, 2764, 2788
Eco57I	CTGAAG	2 cut(s)	54, 615
EcoNI	CCTNNNNNAGG	1 cut(s)	2150
EcoO109I	RGGNCCY	3 cut(s)	154, 208, 2764
EcoRII	CCWGG	4 cut(s)	909, 2053, 2547, 2658
EcoRV	GATATC	2 cut(s)	738, 2127
EcoT14I	CCWWGG	9 cut(s)	517, 1362, 2097, 2576, 2597, 2716, 2733, 2784, 2847
EcoT22I	ATGCAT	1 cut(s)	2451
EcoT38I	GRGCYC	2 cut(s)	851, 2010
EgeI	GGCGCC	1 cut(s)	2595
EheI	GGCGCC	1 cut(s)	2595
ErhI	CCWWGG	9 cut(s)	517, 1362, 2097, 2576, 2597, 2716, 2733, 2784, 2847
Esp3I	CGTCTC	1 cut(s)	1222
FalI	AAGNNNNNCTT	2 cut(s)	1926, 1958
FaqI	GGGAC	1 cut(s)	2471
FauI	CCCGC	1 cut(s)	2062
FauNDI	CATATG	1 cut(s)	1849
FbaI	TGATCA	3 cut(s)	1477, 1557, 2409
FokI	GGATG	7 cut(s)	848, 2015, 2336, 2708, 2923, 2933, 3049
FriOI	GRGCYC	2 cut(s)	851, 2010
FspBI	CTAG	7 cut(s)	518, 723, 1910, 2426, 2513, 2792, 2900
GlaI	GCGC	6 cut(s)	119, 258, 1684, 1908, 2025, 2595
GsaI	CCCAGC	1 cut(s)	531
HaeII	RGCGCY	4 cut(s)	121, 260, 1910, 2597
HapII	CCGG	4 cut(s)	123, 2418, 2591, 2725
HgaI	GACGC	2 cut(s)	1707, 1932
HhaI	GCGC	6 cut(s)	120, 259, 1685, 1909, 2026, 2596
Hin1I	GRCGYC	2 cut(s)	1924, 2594
Hin6I	GCGC	6 cut(s)	118, 257, 1683, 1907, 2024, 2594
HinP1I	GCGC	6 cut(s)	118, 257, 1683, 1907, 2024, 2594
HincII	GTYRAC	2 cut(s)	1722, 1896
HindII	GTYRAC	2 cut(s)	1722, 1896
HindIII	AAGCTT	5 cut(s)	1014, 1796, 1959, 2076, 2159
HpaII	CCGG	4 cut(s)	123, 2418, 2591, 2725
Hpy166II	GTNNAC	7 cut(s)	501, 1722, 1742, 1881, 1896, 2494, 2797
Hpy8I	GTNNAC	7 cut(s)	501, 1722, 1742, 1881, 1896, 2494, 2797
HpyCH4III	ACNGT	9 cut(s)	11, 171, 587, 607, 1098, 1216, 1378, 2828, 2931
HpyCH4IV	ACGT	2 cut(s)	1444, 2432
HpyF10VI	GCNNNNNNNGC	6 cut(s)	353, 1958, 2075, 2514, 2680, 2852
HpySE526I	ACGT	2 cut(s)	1444, 2432
Hsp92I	GRCGYC	2 cut(s)	1924, 2594
HspAI	GCGC	6 cut(s)	118, 257, 1683, 1907, 2024, 2594
KasI	GGCGCC	1 cut(s)	2593
Kpn2I	TCCGGA	1 cut(s)	122
KpnI	GGTACC	1 cut(s)	2733
Ksp22I	TGATCA	3 cut(s)	1477, 1557, 2409
Kzo9I	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
LguI	GCTCTTC	1 cut(s)	1932
LmnI	GCTCC	6 cut(s)	125, 556, 793, 2173, 2280, 2551
Lsp1109I	GCAGC	8 cut(s)	223, 274, 1686, 1692, 1708, 2315, 2529, 2941
MaeI	CTAG	7 cut(s)	518, 723, 1910, 2426, 2513, 2792, 2900
MaeII	ACGT	2 cut(s)	1444, 2432
MalI	GATC	9 cut(s)	217, 231, 813, 1335, 1479, 1559, 1599, 2037, 2411
MboI	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
MfeI	CAATTG	2 cut(s)	855, 1842
MflI	RGATCY	2 cut(s)	215, 1333
MhlI	GDGCHC	7 cut(s)	358, 851, 2010, 2146, 2178, 2285, 3028
MlsI	TGGCCA	1 cut(s)	2294
MluNI	TGGCCA	1 cut(s)	2294
Mly113I	GGCGCC	1 cut(s)	2594
MlyI	GAGTC	9 cut(s)	46, 573, 997, 1481, 1926, 2082, 2453, 2575, 2596
MmeI	TCCRAC	1 cut(s)	2898
Mox20I	TGGCCA	1 cut(s)	2294
Mph1103I	ATGCAT	1 cut(s)	2451
MroI	TCCGGA	1 cut(s)	122
MscI	TGGCCA	1 cut(s)	2294
MseI	TTAA	9 cut(s)	69, 1457, 1800, 1812, 1944, 2049, 2180, 2319, 2769
MslI	CAYNNNNRTG	8 cut(s)	585, 1226, 1583, 1661, 1707, 1749, 2288, 2870
Msp20I	TGGCCA	1 cut(s)	2294
MspA1I	CMGCKG	4 cut(s)	81, 287, 527, 1699
MspCI	CTTAAG	2 cut(s)	68, 1799
MspI	CCGG	4 cut(s)	123, 2418, 2591, 2725
MspR9I	CCNGG	6 cut(s)	911, 2055, 2419, 2549, 2660, 2726
MunI	CAATTG	2 cut(s)	855, 1842
Mva1269I	GAATGC	3 cut(s)	349, 1275, 2449
MvaI	CCWGG	4 cut(s)	911, 2055, 2549, 2660
MvnI	CGCG	2 cut(s)	360, 2026
MwoI	GCNNNNNNNGC	6 cut(s)	353, 1958, 2075, 2514, 2680, 2852
NarI	GGCGCC	1 cut(s)	2594
NciI	CCSGG	2 cut(s)	2419, 2726
NcoI	CCATGG	4 cut(s)	1362, 2097, 2733, 2784
NdeI	CATATG	1 cut(s)	1849
NdeII	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
NlaIV	GGNNCC	7 cut(s)	127, 156, 1213, 2339, 2553, 2595, 2731
NmuCI	GTSAC	5 cut(s)	973, 1357, 1576, 2017, 2192
NsiI	ATGCAT	1 cut(s)	2451
NspI	RCATGY	1 cut(s)	1738
NspV	TTCGAA	2 cut(s)	2998, 3096
OliI	CACNNNNGTG	1 cut(s)	1226
PagI	TCATGA	1 cut(s)	298
PaqCI	CACCTGC	1 cut(s)	2860
PceI	AGGCCT	1 cut(s)	29
PciI	ACATGT	1 cut(s)	1734
PciSI	GCTCTTC	1 cut(s)	1932
PctI	GAATGC	3 cut(s)	349, 1275, 2449
PfeI	GAWTC	6 cut(s)	406, 719, 1342, 2043, 2262, 3098
PflMI	CCANNNNNTGG	1 cut(s)	2527
PleI	GAGTC	9 cut(s)	46, 572, 997, 1480, 1925, 2082, 2453, 2575, 2596
PluTI	GGCGCC	1 cut(s)	2597
PpsI	GAGTC	9 cut(s)	46, 572, 997, 1480, 1925, 2082, 2453, 2575, 2596
Ppu21I	YACGTR	1 cut(s)	1445
PpuMI	RGGWCCY	2 cut(s)	208, 2764
PscI	ACATGT	1 cut(s)	1734
PsiI	TTATAA	4 cut(s)	1610, 1749, 1857, 2615
Psp1406I	AACGTT	1 cut(s)	2432
Psp5II	RGGWCCY	2 cut(s)	208, 2764
Psp6I	CCWGG	4 cut(s)	909, 2053, 2547, 2658
PspFI	CCCAGC	1 cut(s)	527
PspGI	CCWGG	4 cut(s)	909, 2053, 2547, 2658
PspN4I	GGNNCC	7 cut(s)	127, 156, 1213, 2339, 2553, 2595, 2731
PspPI	GGNCC	9 cut(s)	154, 208, 907, 1318, 1366, 2764, 2781, 2788, 2979
PspPPI	RGGWCCY	2 cut(s)	208, 2764
PstNI	CAGNNNCTG	1 cut(s)	287
PsuI	RGATCY	2 cut(s)	215, 1333
PvuII	CAGCTG	2 cut(s)	527, 1699
RsaI	GTAC	6 cut(s)	13, 500, 1104, 1127, 2239, 2731
RsaNI	GTAC	6 cut(s)	12, 499, 1103, 1126, 2238, 2730
RseI	CAYNNNNRTG	8 cut(s)	585, 1226, 1583, 1661, 1707, 1749, 2288, 2870
SapI	GCTCTTC	1 cut(s)	1932
SaqAI	TTAA	9 cut(s)	69, 1457, 1800, 1812, 1944, 2049, 2180, 2319, 2769
Sau3AI	GATC	9 cut(s)	215, 229, 811, 1333, 1477, 1557, 1597, 2035, 2409
Sau96I	GGNCC	9 cut(s)	154, 208, 907, 1318, 1366, 2764, 2781, 2788, 2979
ScaI	AGTACT	1 cut(s)	1104
SchI	GAGTC	9 cut(s)	46, 573, 997, 1481, 1926, 2082, 2453, 2575, 2596
ScrFI	CCNGG	6 cut(s)	911, 2055, 2419, 2549, 2660, 2726
SduI	GDGCHC	7 cut(s)	358, 851, 2010, 2146, 2178, 2285, 3028
SfoI	GGCGCC	1 cut(s)	2595
SfuI	TTCGAA	2 cut(s)	2998, 3096
SinI	GGWCC	4 cut(s)	208, 1318, 2764, 2788
SmiMI	CAYNNNNRTG	8 cut(s)	585, 1226, 1583, 1661, 1707, 1749, 2288, 2870
SmlI	CTYRAG	6 cut(s)	68, 389, 1206, 1799, 2562, 2583
SmoI	CTYRAG	6 cut(s)	68, 389, 1206, 1799, 2562, 2583
SseBI	AGGCCT	1 cut(s)	29
SsiI	CCGC	8 cut(s)	79, 142, 278, 285, 360, 728, 1247, 2069
SspDI	GGCGCC	1 cut(s)	2593
SspI	AATATT	1 cut(s)	2746
SspMI	CTAG	7 cut(s)	518, 723, 1910, 2426, 2513, 2792, 2900
StuI	AGGCCT	1 cut(s)	29
StyD4I	CCNGG	6 cut(s)	909, 2053, 2417, 2547, 2658, 2724
StyI	CCWWGG	9 cut(s)	517, 1362, 2097, 2576, 2597, 2716, 2733, 2784, 2847
TaaI	ACNGT	9 cut(s)	11, 171, 587, 607, 1098, 1216, 1378, 2828, 2931
TaiI	ACGT	2 cut(s)	1447, 2435
TaqI	TCGA	5 cut(s)	916, 942, 1834, 2998, 3096
TatI	WGTACW	3 cut(s)	498, 1102, 1125
TauI	GCSGC	3 cut(s)	81, 280, 287
TfiI	GAWTC	6 cut(s)	406, 719, 1342, 2043, 2262, 3098
Tru1I	TTAA	9 cut(s)	69, 1457, 1800, 1812, 1944, 2049, 2180, 2319, 2769
Tru9I	TTAA	9 cut(s)	69, 1457, 1800, 1812, 1944, 2049, 2180, 2319, 2769
TscAI	CASTG	6 cut(s)	354, 1585, 1959, 2290, 2911, 2936
TseFI	GTSAC	5 cut(s)	973, 1357, 1576, 2017, 2192
TseI	GCWGC	8 cut(s)	236, 287, 1680, 1696, 1699, 2328, 2517, 2954
Tsp45I	GTSAC	5 cut(s)	973, 1357, 1576, 2017, 2192
TspGWI	ACGGA	1 cut(s)	2119
TspRI	CASTG	6 cut(s)	354, 1585, 1959, 2290, 2911, 2936
Van91I	CCANNNNNTGG	1 cut(s)	2527
Vha464I	CTTAAG	2 cut(s)	68, 1799
VpaK11BI	GGWCC	4 cut(s)	208, 1318, 2764, 2788
XagI	CCTNNNNNAGG	1 cut(s)	2150
XapI	RAATTY	6 cut(s)	548, 689, 1407, 1782, 2394, 3007
XceI	RCATGY	1 cut(s)	1738
XmaJI	CCTAGG	1 cut(s)	517
XspI	CTAG	7 cut(s)	518, 723, 1910, 2426, 2513, 2792, 2900
ZrmI	AGTACT	1 cut(s)	1104
Zsp2I	ATGCAT	1 cut(s)	2451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.