Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Reverse (-)

9727127 .. 9730318

Gene Structure 3192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1113500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3093 bp

ATGGTCGTCGAGCCAAATACAGAGGATCAAGCTACTGAAGGTGCCTCCTCTCTTACTGCTGGCTTGCTTATACAGACCATCGAGCACCGGGGAACCTCGTTTAGACGTATTGTTCTAGGCATTTTGTTTTCATCGCGCATTAGAAAAACCACACGTCTGCGCAAAGCCTCTAGTTATGAACCTTTAGCAACAGCACCAACGGCACCTAATGCTGCCTCTGACCATGTGATTGACATCCCTCCCCAGGAAAGGTTTTCGAACAAGGTTGCCAGAATTGTGAGGGAGAAGGACTTGAATTCATTCCGCAGACTTGGTGGTGTTGACGGCATCTTGCCACTTCTTAGGTCTCAATTTGAGGATGGTGCCGTTGATGGTGGTCAAAATCCACAAGGATGGTACGTTACCAAGTCCCCAGTTGATGCAAAAGGTTTCTTGTACTTTTTGTTTGAGGCGTGCAATCAGTACACGATTTTTTTCCTCTTGCTTTCAGCTGGGCTTTCATTCGCCATCGAATTCATGACGCAAGGAGTCAAGTATGGCTGGCATGACGGTGTTGGCATACTAATTGCGGCGCTCTTACTTATTGCTTTCCCTTCTGTGGGGAACTTCCGCTATCAGAGAAAGCTGGCGCGGAAGCACTTGCTGGATAGGAGCAAACTGATGGTGAATGTTGAACGAAGTAGCACACCAATTACCATATCTGGCGTTGTGGTGGGTGATATAGTTCATTTGCAGGAAGGAGACCGTGTTCCTGCTGACGGTTTGTTCATAGATCATGATGAGGAACTGGTGCTGGATGATGTATTGAGACTAAAAATTGATCGTGAAAATAATCCATTTGTTTTTTCGGGTTCAATGGTTGTGAAGGGCCGCGGCCGCATGGTTGTTACATCCATTAGTGCTAATACAGCTTTTGCTGAGGTGCTGAGCTTGGTGGCTGATCATAATCCAAAGGCGAAGACACTACTACAAGCTTTGATGGACAAACCGAATGCATGTATGGATTATCTTGCATGTTGTGTCTCGATACTGATTGCGCTTGTGATGTTGATACGCCTACTATTCTTCAAGAAGCACGAAAACTACAATGAGAGGCCAGAGCTGAAAGGGGAAGTTTCAATGAATCTTATGATGAGGACCATTGAGAAGATCTTCTTGAAACCTCAAGGAAAAATTTCCATTTTAGCAAGTGTTCTAGCAACCGCGGTAATAGGGATACAACATGGGATGCATTTTGTGATTGCAGTTGCCCTTTCTCAATGGAGCGAGAAGGCGGCCAAAAATAAGGCAGTACCACAGAATCTATCAGCTTGTGTAACCATGGGACTCATAAATGTCATCTGCATCGAAACAACTGCAGAGTTAATGTGCAAAGCGACGGAGGTCAAGGAATTTTGGATTGGTGAAAAGGATTTAAGCATTGGTGAAGTGGACTCGGAAATTGACCAAGTTCTTGAGGTACTGCATCAGGGGATTTCAGCTACCGCAGTTACAGCGTCACCAACAAATGATCTGCTCATTTCTTGGCTGAACACCAGATGGGGTAGTAATGCGGCATTATTGGATCGAGGATTTGAAACCGTTGAAGAAAGACAATTGAGCTCTAACAAGAAATGTAGAGGAATCTTGGTGAGGAAAATCGAGAATGAGCAGATTATGCAACTTCACTGCGACGGAGATGCATCAACAATATTAGGCATGTGTTCACATTACTATGATAACAGAGGGGAAAGTCACAATATCGGAAACCAGAAGAGAAAACTTGAGCAGGTGATCAAGAAGATGGAGGAGGAGGGTCTGAGACCGATTGCATACGCGTACAAGAAAACAGAAGAATTTGCAGAAGATGGGTTGATTTTGTTGGCATTTGTCGGTCTTAGATGTCCATATCAAGAAGAGCTTAAAGTGGCAGTGGAAGCTCTTAGAGAAGCTGGAGTAAGCATCAAACTGGTGTCAGAGGATGAGCTCTCAATAGTGAGAGATACAGCTTCGGAACTTGGGATCTCCCCTGCTTCTGATGACAAGGAAATTGAAGGTCAAGCTTTCCGAAGACTCAATTCCATGGAAAGGCCGGATGCGGTGGATTTGATCTCTGTGATGGGAAGTTCCATCCCCAAGGACAAGTTTCTCATGGTGGAGGGGCTAAGGAAAAAAGGTCGCATAGTTGCATTCTGTGGAGGTTTGACCGTACGCGACACTCTGACCTTAAAAGAAGCTGATGTAGGAATCGTGCACGATATCTGGAGAACCGGGATGGCCAGAGAGAATGCCGATCTTATAATCAGCAGCGGCTGTTTTTTAAGCAAGATTATCAAGTCTGGTGCTTGTGCCTACCACAACATTCAGCAGTTCTCTCAACTTCAGCTCACCGCTTCCATATCCGGTTTACTTGTAACCCTAGTCGCCACAATGCATTCGGGAGAGTCCCCTCTAACAGCAGTTCATTTGATTTGGGTGAACTTGATCATGTGCCTTCTTGGTGGCCTCATGATGGTGATGGAGCTACAAGGCACCTTAGAACACATTCAAAGACCTGAGGGGAGGACCGTGTCGCTTATAACCAAAGTCATCTGGAGAAACATAGCAGTCCAAGTTTCATATCAGGTTTCCGTCTTGCTGATCTTACATTTCATGCCGAGCATGAATGAAGGCGTCCGGAATACCATGATTTTCAGTACCTTCACCTTATGCCAGGTCCTTAATCTGTTCAGTGCCATGGACTTGGTGAAAAAGGAAGTGCTGGTTGTAGTCCTCCACAGCCATTGGTTTCTGATGGCGTTGGGGGCTGTGTTGACAATGCAGGTCATCGTTGTTGAGTTCGGAAAAGGACTAGCTAGCTGTGTGAAGTTGAATGCACTGCAGTGGCTAATCTGTTTCACTCTGGCTGCTCTTTCGTGGGGTTCTGATCGGGCCATCAAATTCCTTTCAGCTCACATCCAAAGAGCAACTTCGGCACTGATGGTGAGCTCACACGTTGAATTCTCACATCGGCACCGCAGATTATACATTCTTATTTGGTTGTTCCTCATCTTCTCTGTATCATCATACTGTTTCCACCCAGAAAATCATCTACTCTCGCTGTCTATCCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1031

Amino Acids

114.42

Weight (kDa)

7.15

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	226 - 312	4.2e-10	P-type ATPase actuator domain
Cation_ATPase	PF13246	517 - 632	3.2e-08	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	809 - 974	9e-22	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2279, 2558
AarI	CACCTGC	1 cut(s)	1759
Acc16I	TGCGCA	1 cut(s)	161
Acc36I	ACCTGC	2 cut(s)	1759, 2791
AccB1I	GGYRCC	5 cut(s)	41, 202, 362, 2510, 2991
AccII	CGCG	6 cut(s)	136, 631, 873, 1205, 1817, 2193
AccIII	TCCGGA	1 cut(s)	2655
AclWI	GGATC	3 cut(s)	33, 1572, 2009
AcoI	YGGCCR	3 cut(s)	874, 1275, 2256
AcsI	RAATTY	7 cut(s)	295, 512, 1173, 1391, 1835, 2918, 2978
AcuI	CTGAAG	2 cut(s)	57, 2345
AcyI	GRCGYC	1 cut(s)	2652
AfaI	GTAC	8 cut(s)	398, 437, 464, 1293, 1461, 1820, 2190, 2677
AfiI	CCNNNNNNNGG	7 cut(s)	244, 249, 598, 599, 758, 2067, 2491
AflIII	ACRYGT	3 cut(s)	152, 1815, 2971
AjiI	CACGTC	1 cut(s)	155
AjnI	CCWGG	2 cut(s)	243, 2691
AjuI	GAANNNNNNNTTGG	2 cut(s)	1383, 1415
AleI	CACNNNNGTG	1 cut(s)	2860
AloI	GAACNNNNNNTCC	2 cut(s)	732, 764
Alw21I	GWGCWC	5 cut(s)	87, 1604, 1968, 2235, 2969
Alw26I	GTCTC	5 cut(s)	351, 735, 802, 1027, 1795
Alw44I	GTGCAC	1 cut(s)	2231
AlwI	GGATC	3 cut(s)	33, 1572, 2009
AlwNI	CAGNNNCTG	1 cut(s)	2292
Aor13HI	TCCGGA	1 cut(s)	2655
AoxI	GGCC	8 cut(s)	868, 874, 1094, 1275, 2069, 2256, 2482, 2910
ApaLI	GTGCAC	1 cut(s)	2231
ApeKI	GCWGC	3 cut(s)	212, 2286, 2885
ApoI	RAATTY	7 cut(s)	295, 512, 1173, 1391, 1835, 2918, 2978
Asp700I	GAANNNNTTC	4 cut(s)	299, 1151, 1174, 2523
AspLEI	GCGC	5 cut(s)	138, 162, 574, 631, 1039
AspS9I	GGNCC	5 cut(s)	868, 1137, 2544, 2695, 2910
AsuC2I	CCSGG	2 cut(s)	89, 2251
AsuII	TTCGAA	1 cut(s)	257
AsuNHI	GCTAGC	1 cut(s)	2834
AvaII	GGWCC	3 cut(s)	1137, 2544, 2695
AxyI	CCTNAGG	1 cut(s)	2535
BaeGI	GKGCMC	1 cut(s)	2235
BalI	TGGCCA	1 cut(s)	2258
BanI	GGYRCC	5 cut(s)	41, 202, 362, 2510, 2991
BanII	GRGCYC	3 cut(s)	1604, 1968, 2969
BbsI	GAAGAC	2 cut(s)	965, 2056
Bbv12I	GWGCWC	5 cut(s)	87, 1604, 1968, 2235, 2969
BbvCI	CCTCAGC	1 cut(s)	918
BbvI	GCAGC	3 cut(s)	199, 2298, 2872
BceAI	ACGGC	3 cut(s)	216, 340, 350
BcgI	CGANNNNNNTGC	4 cut(s)	1337, 1371, 1661, 1695
BciT130I	CCWGG	2 cut(s)	245, 2693
BciVI	GTATCC	1 cut(s)	1209
BclI	TGATCA	3 cut(s)	940, 1773, 2463
BcnI	CCSGG	2 cut(s)	89, 2251
BcoDI	GTCTC	5 cut(s)	351, 735, 802, 1027, 1795
BfaI	CTAG	6 cut(s)	116, 171, 1196, 2399, 2831, 2835
BfmI	CTRYAG	2 cut(s)	1356, 2858
BfoI	RGCGCY	1 cut(s)	575
BfuAI	ACCTGC	2 cut(s)	1759, 2791
BfuI	GTATCC	1 cut(s)	1209
BglII	AGATCT	1 cut(s)	1149
BlpI	GCTNAGC	1 cut(s)	926
Bme1390I	CCNGG	4 cut(s)	89, 245, 2251, 2693
Bme18I	GGWCC	3 cut(s)	1137, 2544, 2695
BmgBI	CACGTC	1 cut(s)	155
BmgT120I	GGNCC	5 cut(s)	868, 1137, 2544, 2695, 2910
BmiI	GGNNCC	6 cut(s)	43, 94, 204, 364, 2512, 2993
BmrFI	CCNGG	4 cut(s)	89, 245, 2251, 2693
BmrI	ACTGGG	1 cut(s)	407
BmsI	GCATC	9 cut(s)	336, 409, 1218, 1353, 1474, 1669, 1691, 1950, 2065
BmtI	GCTAGC	1 cut(s)	2838
BmuI	ACTGGG	1 cut(s)	407
BoxI	GACNNNNGTC	1 cut(s)	1382
BpiI	GAAGAC	2 cut(s)	965, 2056
BpmI	CTGGAG	3 cut(s)	1953, 2263, 2593
Bpu10I	CCTNAGC	2 cut(s)	918, 2144
Bpu1102I	GCTNAGC	1 cut(s)	926
Bpu14I	TTCGAA	1 cut(s)	257
BpuEI	CTTGAG	3 cut(s)	1149, 1475, 1784
BpuMI	CCSGG	2 cut(s)	89, 2251
BsaBI	GATNNNNATC	2 cut(s)	233, 945
BsaHI	GRCGYC	1 cut(s)	2652
BsaI	GGTCTC	3 cut(s)	351, 735, 1795
BsaJI	CCNNGG	8 cut(s)	88, 243, 871, 1203, 1320, 2061, 2115, 2715
BsaWI	WCCGGW	2 cut(s)	2381, 2655
BsaXI	ACNNNNNCTCC	2 cut(s)	732, 762
Bsc4I	CCNNNNNNNGG	7 cut(s)	244, 249, 598, 599, 758, 2067, 2491
Bse1I	ACTGG	3 cut(s)	413, 792, 1953
Bse21I	CCTNAGG	1 cut(s)	2535
Bse8I	GATNNNNATC	2 cut(s)	233, 945
BseAI	TCCGGA	1 cut(s)	2655
BseBI	CCWGG	2 cut(s)	245, 2693
BseDI	CCNNGG	8 cut(s)	88, 243, 871, 1203, 1320, 2061, 2115, 2715
BseJI	GATNNNNATC	2 cut(s)	233, 945
BseLI	CCNNNNNNNGG	7 cut(s)	244, 249, 598, 599, 758, 2067, 2491
BseMII	CTCAG	4 cut(s)	909, 917, 1790, 2526
BseNI	ACTGG	3 cut(s)	413, 792, 1953
BseRI	GAGGAG	3 cut(s)	37, 1802, 1805
BseSI	GKGCMC	1 cut(s)	2235
BseX3I	CGGCCG	1 cut(s)	874
BseXI	GCAGC	3 cut(s)	199, 2298, 2872
BseYI	CCCAGC	1 cut(s)	491
Bsh1236I	CGCG	6 cut(s)	136, 631, 873, 1205, 1817, 2193
Bsh1285I	CGRYCG	1 cut(s)	877
BshFI	GGCC	8 cut(s)	870, 876, 1096, 1277, 2071, 2258, 2484, 2912
BshNI	GGYRCC	5 cut(s)	41, 202, 362, 2510, 2991
BsiEI	CGRYCG	1 cut(s)	877
BsiHKAI	GWGCWC	5 cut(s)	87, 1604, 1968, 2235, 2969
BsiSI	CCGG	5 cut(s)	88, 2072, 2250, 2382, 2656
BsiWI	CGTACG	1 cut(s)	2188
BslFI	GGGAC	3 cut(s)	394, 1338, 2410
BslI	CCNNNNNNNGG	7 cut(s)	244, 249, 598, 599, 758, 2067, 2491
BsmAI	GTCTC	5 cut(s)	351, 735, 802, 1027, 1795
BsmFI	GGGAC	3 cut(s)	394, 1338, 2410
BsmI	GAATGC	5 cut(s)	997, 2168, 2272, 2413, 2857
BsnI	GGCC	8 cut(s)	870, 876, 1096, 1277, 2071, 2258, 2484, 2912
Bso31I	GGTCTC	3 cut(s)	351, 735, 1795
Bsp119I	TTCGAA	1 cut(s)	257
Bsp1286I	GDGCHC	5 cut(s)	87, 1604, 1968, 2235, 2969
Bsp13I	TCCGGA	1 cut(s)	2655
Bsp1720I	GCTNAGC	1 cut(s)	926
Bsp19I	CCATGG	3 cut(s)	1320, 2061, 2715
BspANI	GGCC	8 cut(s)	870, 876, 1096, 1277, 2071, 2258, 2484, 2912
BspCNI	CTCAG	4 cut(s)	910, 918, 1791, 2527
BspEI	TCCGGA	1 cut(s)	2655
BspFNI	CGCG	6 cut(s)	136, 631, 873, 1205, 1817, 2193
BspHI	TCATGA	3 cut(s)	516, 775, 2487
BspLI	GGNNCC	6 cut(s)	43, 94, 204, 364, 2512, 2993
BspMAI	CTGCAG	2 cut(s)	1360, 2862
BspMI	ACCTGC	2 cut(s)	1759, 2791
BspOI	GCTAGC	1 cut(s)	2838
BspPI	GGATC	3 cut(s)	33, 1572, 2009
BspQI	GCTCTTC	1 cut(s)	1890
BspT104I	TTCGAA	1 cut(s)	257
BspT107I	GGYRCC	5 cut(s)	41, 202, 362, 2510, 2991
BspTNI	GGTCTC	3 cut(s)	351, 735, 1795
BsrI	ACTGG	3 cut(s)	413, 792, 1953
BssECI	CCNNGG	8 cut(s)	88, 243, 871, 1203, 1320, 2061, 2115, 2715
BssNI	GRCGYC	1 cut(s)	2652
BssT1I	CCWWGG	4 cut(s)	1320, 2061, 2115, 2715
Bst2UI	CCWGG	2 cut(s)	245, 2693
Bst4CI	ACNGT	7 cut(s)	551, 746, 761, 1582, 2188, 2548, 3050
Bst6I	CTCTTC	2 cut(s)	1748, 1890
BstACI	GRCGYC	1 cut(s)	2652
BstAPI	GCANNNNNTGC	2 cut(s)	209, 1657
BstBI	TTCGAA	1 cut(s)	257
BstC8I	GCNNGC	6 cut(s)	61, 65, 454, 542, 627, 2836
BstDEI	CTNAG	9 cut(s)	341, 918, 926, 1799, 1877, 1922, 2144, 2515, 2535
BstDSI	CCRYGG	5 cut(s)	871, 1203, 1320, 2061, 2715
BstFNI	CGCG	6 cut(s)	136, 631, 873, 1205, 1817, 2193
BstH2I	RGCGCY	1 cut(s)	575
BstHHI	GCGC	5 cut(s)	138, 162, 574, 631, 1039
BstMAI	GTCTC	5 cut(s)	351, 735, 802, 1027, 1795
BstMCI	CGRYCG	1 cut(s)	877
BstMWI	GCNNNNNNNGC	9 cut(s)	200, 209, 876, 908, 1493, 1657, 1916, 2783, 2951
BstNI	CCWGG	2 cut(s)	245, 2693
BstNSI	RCATGY	3 cut(s)	999, 1017, 1702
BstPAI	GACNNNNGTC	1 cut(s)	1382
BstSCI	CCNGG	4 cut(s)	87, 243, 2249, 2691
BstSFI	CTRYAG	2 cut(s)	1356, 2858
BstSLI	GKGCMC	1 cut(s)	2235
BstUI	CGCG	6 cut(s)	136, 631, 873, 1205, 1817, 2193
BstV1I	GCAGC	3 cut(s)	199, 2298, 2872
BstV2I	GAAGAC	2 cut(s)	965, 2056
BstX2I	RGATCY	2 cut(s)	1149, 2001
BstXI	CCANNNNNNTGG	2 cut(s)	393, 2722
BstYI	RGATCY	2 cut(s)	1149, 2001
BstZI	CGGCCG	1 cut(s)	874
Bsu36I	CCTNAGG	1 cut(s)	2535
BsuI	GTATCC	1 cut(s)	1209
BsuRI	GGCC	8 cut(s)	870, 876, 1096, 1277, 2071, 2258, 2484, 2912
BtgI	CCRYGG	5 cut(s)	871, 1203, 1320, 2061, 2715
BtgZI	GCGATG	1 cut(s)	117
BtrI	CACGTC	1 cut(s)	155
BtsI	GCAGTG	4 cut(s)	1666, 1917, 2855, 2867
BtsIMutI	CAGTG	6 cut(s)	1666, 1917, 2716, 2855, 2867, 2954
BveI	ACCTGC	2 cut(s)	1759, 2791
Cac8I	GCNNGC	6 cut(s)	61, 65, 454, 542, 627, 2836
CaiI	CAGNNNCTG	1 cut(s)	2292
CciI	TCATGA	3 cut(s)	516, 775, 2487
CciNI	GCGGCCGC	1 cut(s)	874
CfoI	GCGC	5 cut(s)	138, 162, 574, 631, 1039
Cfr13I	GGNCC	5 cut(s)	868, 1137, 2544, 2695, 2910
Cfr42I	CCGCGG	2 cut(s)	874, 1206
CseI	GACGC	3 cut(s)	529, 1485, 2641
Csp6I	GTAC	8 cut(s)	397, 436, 463, 1292, 1460, 1819, 2189, 2676
CspCI	CAANNNNNGTGG	2 cut(s)	2744, 2779
CviQI	GTAC	8 cut(s)	397, 436, 463, 1292, 1460, 1819, 2189, 2676
DdeI	CTNAG	9 cut(s)	341, 918, 926, 1799, 1877, 1922, 2144, 2515, 2535
EaeI	YGGCCR	3 cut(s)	874, 1275, 2256
EagI	CGGCCG	1 cut(s)	874
Eam1104I	CTCTTC	2 cut(s)	1748, 1890
EarI	CTCTTC	2 cut(s)	1748, 1890
Ecl136II	GAGCTC	3 cut(s)	1602, 1966, 2967
EclXI	CGGCCG	1 cut(s)	874
Eco130I	CCWWGG	4 cut(s)	1320, 2061, 2115, 2715
Eco24I	GRGCYC	3 cut(s)	1604, 1968, 2969
Eco31I	GGTCTC	3 cut(s)	351, 735, 1795
Eco32I	GATATC	1 cut(s)	2239
Eco47I	GGWCC	3 cut(s)	1137, 2544, 2695
Eco52I	CGGCCG	1 cut(s)	874
Eco53kI	GAGCTC	3 cut(s)	1602, 1966, 2967
Eco57I	CTGAAG	2 cut(s)	57, 2345
Eco81I	CCTNAGG	1 cut(s)	2535
EcoICRI	GAGCTC	3 cut(s)	1602, 1966, 2967
EcoO109I	RGGNCCY	1 cut(s)	2695
EcoRI	GAATTC	3 cut(s)	295, 512, 2978
EcoRII	CCWGG	2 cut(s)	243, 2691
EcoRV	GATATC	1 cut(s)	2239
EcoT14I	CCWWGG	4 cut(s)	1320, 2061, 2115, 2715
EcoT22I	ATGCAT	4 cut(s)	997, 1233, 1684, 2415
EcoT38I	GRGCYC	3 cut(s)	1604, 1968, 2969
ErhI	CCWWGG	4 cut(s)	1320, 2061, 2115, 2715
FalI	AAGNNNNNCTT	6 cut(s)	1139, 1171, 1884, 1916, 2932, 2964
FaqI	GGGAC	3 cut(s)	394, 1338, 2410
FbaI	TGATCA	3 cut(s)	940, 1773, 2463
FriOI	GRGCYC	3 cut(s)	1604, 1968, 2969
FspBI	CTAG	6 cut(s)	116, 171, 1196, 2399, 2831, 2835
FspI	TGCGCA	1 cut(s)	161
GlaI	GCGC	5 cut(s)	137, 161, 573, 630, 1038
GsaI	CCCAGC	1 cut(s)	495
GsuI	CTGGAG	3 cut(s)	1953, 2263, 2593
HaeII	RGCGCY	1 cut(s)	575
HaeIII	GGCC	8 cut(s)	870, 876, 1096, 1277, 2071, 2258, 2484, 2912
HapII	CCGG	5 cut(s)	88, 2072, 2250, 2382, 2656
HgaI	GACGC	3 cut(s)	529, 1485, 2641
HhaI	GCGC	5 cut(s)	138, 162, 574, 631, 1039
Hin1I	GRCGYC	1 cut(s)	2652
Hin6I	GCGC	5 cut(s)	136, 160, 572, 629, 1037
HinP1I	GCGC	5 cut(s)	136, 160, 572, 629, 1037
HincII	GTYRAC	2 cut(s)	322, 2793
HindII	GTYRAC	2 cut(s)	322, 2793
HindIII	AAGCTT	2 cut(s)	972, 2040
HinfI	GANTC	9 cut(s)	528, 1123, 1300, 1326, 1433, 1623, 2052, 2226, 2423
HpaII	CCGG	5 cut(s)	88, 2072, 2250, 2382, 2656
Hpy166II	GTNNAC	8 cut(s)	322, 465, 1432, 1706, 2233, 2387, 2458, 2793
Hpy8I	GTNNAC	8 cut(s)	322, 465, 1432, 1706, 2233, 2387, 2458, 2793
Hpy99I	CGWCG	3 cut(s)	11, 1381, 1676
HpyCH4III	ACNGT	7 cut(s)	551, 746, 761, 1582, 2188, 2548, 3050
HpyCH4IV	ACGT	4 cut(s)	106, 154, 399, 2973
HpyF10VI	GCNNNNNNNGC	9 cut(s)	200, 209, 876, 908, 1493, 1657, 1916, 2783, 2951
HpyF3I	CTNAG	9 cut(s)	341, 918, 926, 1799, 1877, 1922, 2144, 2515, 2535
HpySE526I	ACGT	4 cut(s)	106, 154, 399, 2973
Hsp92I	GRCGYC	1 cut(s)	2652
HspAI	GCGC	5 cut(s)	136, 160, 572, 629, 1037
Kpn2I	TCCGGA	1 cut(s)	2655
Ksp22I	TGATCA	3 cut(s)	940, 1773, 2463
KspI	CCGCGG	2 cut(s)	874, 1206
LguI	GCTCTTC	1 cut(s)	1890
LmnI	GCTCC	3 cut(s)	651, 1263, 2500
Lsp1109I	GCAGC	3 cut(s)	199, 2298, 2872
LweI	GCATC	9 cut(s)	336, 409, 1218, 1353, 1474, 1669, 1691, 1950, 2065
MaeI	CTAG	6 cut(s)	116, 171, 1196, 2399, 2831, 2835
MaeII	ACGT	4 cut(s)	106, 154, 399, 2973
MaeIII	GTNAC	7 cut(s)	400, 886, 1315, 1489, 1497, 1733, 2392
MfeI	CAATTG	1 cut(s)	1595
MflI	RGATCY	2 cut(s)	1149, 2001
MhlI	GDGCHC	5 cut(s)	87, 1604, 1968, 2235, 2969
MlsI	TGGCCA	1 cut(s)	2258
MluI	ACGCGT	1 cut(s)	1815
MluNI	TGGCCA	1 cut(s)	2258
MlyI	GAGTC	5 cut(s)	537, 1320, 1427, 2046, 2432
Mox20I	TGGCCA	1 cut(s)	2258
Mph1103I	ATGCAT	4 cut(s)	997, 1233, 1684, 2415
MroI	TCCGGA	1 cut(s)	2655
MroXI	GAANNNNTTC	4 cut(s)	299, 1151, 1174, 2523
MscI	TGGCCA	1 cut(s)	2258
MseI	TTAA	6 cut(s)	1364, 1415, 1902, 2207, 2300, 2700
MslI	CAYNNNNRTG	5 cut(s)	391, 549, 1713, 2492, 2860
Msp20I	TGGCCA	1 cut(s)	2258
MspA1I	CMGCKG	5 cut(s)	491, 873, 1205, 2289, 3090
MspI	CCGG	5 cut(s)	88, 2072, 2250, 2382, 2656
MspR9I	CCNGG	4 cut(s)	89, 245, 2251, 2693
MunI	CAATTG	1 cut(s)	1595
Mva1269I	GAATGC	5 cut(s)	997, 2168, 2272, 2413, 2857
MvaI	CCWGG	2 cut(s)	245, 2693
MvnI	CGCG	6 cut(s)	136, 631, 873, 1205, 1817, 2193
MwoI	GCNNNNNNNGC	9 cut(s)	200, 209, 876, 908, 1493, 1657, 1916, 2783, 2951
NciI	CCSGG	2 cut(s)	89, 2251
NcoI	CCATGG	3 cut(s)	1320, 2061, 2715
NheI	GCTAGC	1 cut(s)	2834
NlaIV	GGNNCC	6 cut(s)	43, 94, 204, 364, 2512, 2993
NmeAIII	GCCGAG	1 cut(s)	2661
NmuCI	GTSAC	2 cut(s)	1497, 1733
NotI	GCGGCCGC	1 cut(s)	874
NsbI	TGCGCA	1 cut(s)	161
NsiI	ATGCAT	4 cut(s)	997, 1233, 1684, 2415
NspI	RCATGY	3 cut(s)	999, 1017, 1702
NspV	TTCGAA	1 cut(s)	257
OliI	CACNNNNGTG	1 cut(s)	2860
PagI	TCATGA	3 cut(s)	516, 775, 2487
PaqCI	CACCTGC	1 cut(s)	1759
PciSI	GCTCTTC	1 cut(s)	1890
PctI	GAATGC	5 cut(s)	997, 2168, 2272, 2413, 2857
PdmI	GAANNNNTTC	4 cut(s)	299, 1151, 1174, 2523
PfeI	GAWTC	4 cut(s)	1123, 1300, 1623, 2226
Pfl23II	CGTACG	1 cut(s)	2188
PflFI	GACNNNGTC	1 cut(s)	2548
PleI	GAGTC	5 cut(s)	536, 1320, 1427, 2046, 2431
PpsI	GAGTC	5 cut(s)	536, 1320, 1427, 2046, 2431
PpuMI	RGGWCCY	1 cut(s)	2695
PshAI	GACNNNNGTC	1 cut(s)	1382
PsiI	TTATAA	2 cut(s)	2279, 2558
Psp124BI	GAGCTC	3 cut(s)	1604, 1968, 2969
Psp5II	RGGWCCY	1 cut(s)	2695
Psp6I	CCWGG	2 cut(s)	243, 2691
PspFI	CCCAGC	1 cut(s)	491
PspGI	CCWGG	2 cut(s)	243, 2691
PspLI	CGTACG	1 cut(s)	2188
PspN4I	GGNNCC	6 cut(s)	43, 94, 204, 364, 2512, 2993
PspPI	GGNCC	5 cut(s)	868, 1137, 2544, 2695, 2910
PspPPI	RGGWCCY	1 cut(s)	2695
PstI	CTGCAG	2 cut(s)	1360, 2862
PstNI	CAGNNNCTG	1 cut(s)	2292
PsuI	RGATCY	2 cut(s)	1149, 2001
PsyI	GACNNNGTC	1 cut(s)	2548
PvuII	CAGCTG	1 cut(s)	491
RsaI	GTAC	8 cut(s)	398, 437, 464, 1293, 1461, 1820, 2190, 2677
RsaNI	GTAC	8 cut(s)	397, 436, 463, 1292, 1460, 1819, 2189, 2676
RseI	CAYNNNNRTG	5 cut(s)	391, 549, 1713, 2492, 2860
SacI	GAGCTC	3 cut(s)	1604, 1968, 2969
SacII	CCGCGG	2 cut(s)	874, 1206
SapI	GCTCTTC	1 cut(s)	1890
SaqAI	TTAA	6 cut(s)	1364, 1415, 1902, 2207, 2300, 2700
Sau96I	GGNCC	5 cut(s)	868, 1137, 2544, 2695, 2910
SchI	GAGTC	5 cut(s)	537, 1320, 1427, 2046, 2432
ScrFI	CCNGG	4 cut(s)	89, 245, 2251, 2693
SduI	GDGCHC	5 cut(s)	87, 1604, 1968, 2235, 2969
SfaNI	GCATC	9 cut(s)	336, 409, 1218, 1353, 1474, 1669, 1691, 1950, 2065
SfcI	CTRYAG	2 cut(s)	1356, 2858
Sfr303I	CCGCGG	2 cut(s)	874, 1206
SfuI	TTCGAA	1 cut(s)	257
SgrBI	CCGCGG	2 cut(s)	874, 1206
SinI	GGWCC	3 cut(s)	1137, 2544, 2695
SmiMI	CAYNNNNRTG	5 cut(s)	391, 549, 1713, 2492, 2860
SmlI	CTYRAG	3 cut(s)	1164, 1454, 1763
SmoI	CTYRAG	3 cut(s)	1164, 1454, 1763
SspI	AATATT	1 cut(s)	1692
SspMI	CTAG	6 cut(s)	116, 171, 1196, 2399, 2831, 2835
SstI	GAGCTC	3 cut(s)	1604, 1968, 2969
StyD4I	CCNGG	4 cut(s)	87, 243, 2249, 2691
StyI	CCWWGG	4 cut(s)	1320, 2061, 2115, 2715
TaaI	ACNGT	7 cut(s)	551, 746, 761, 1582, 2188, 2548, 3050
TaiI	ACGT	4 cut(s)	109, 157, 402, 2976
TaqI	TCGA	8 cut(s)	9, 81, 257, 510, 1025, 1347, 1567, 1641
TaqII	GACCGA	2 cut(s)	1819, 1862
TatI	WGTACW	2 cut(s)	435, 462
TauI	GCSGC	7 cut(s)	572, 873, 876, 879, 1277, 1556, 2292
TfiI	GAWTC	4 cut(s)	1123, 1300, 1623, 2226
Tru1I	TTAA	6 cut(s)	1364, 1415, 1902, 2207, 2300, 2700
Tru9I	TTAA	6 cut(s)	1364, 1415, 1902, 2207, 2300, 2700
TscAI	CASTG	6 cut(s)	1673, 1917, 2716, 2862, 2867, 2961
TseFI	GTSAC	2 cut(s)	1497, 1733
TseI	GCWGC	3 cut(s)	212, 2286, 2885
Tsp45I	GTSAC	2 cut(s)	1497, 1733
TspGWI	ACGGA	3 cut(s)	1394, 1689, 2599
TspRI	CASTG	6 cut(s)	1673, 1917, 2716, 2862, 2867, 2961
Tth111I	GACNNNGTC	1 cut(s)	2548
VneI	GTGCAC	1 cut(s)	2231
VpaK11BI	GGWCC	3 cut(s)	1137, 2544, 2695
XapI	RAATTY	7 cut(s)	295, 512, 1173, 1391, 1835, 2918, 2978
XceI	RCATGY	3 cut(s)	999, 1017, 1702
XmnI	GAANNNNTTC	4 cut(s)	299, 1151, 1174, 2523
XspI	CTAG	6 cut(s)	116, 171, 1196, 2399, 2831, 2835
Zsp2I	ATGCAT	4 cut(s)	997, 1233, 1684, 2415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.