Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0008877

Physical Location & Seq

Genomic Coordinates Forward (+)

74744832 .. 74748225

Gene Structure 3394 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG520100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3204 bp

ATGCCAAACAGTACCATTGGTGATCAAGCTTCTGAAGGTGTCAGCCACTCGACTTTTACTACTGACCTTATTCAAACCGTTAGTAGTTGTGTCCAATATGAGCAGAGCAAGCAGAGCCGCTGGTATATATACATTGCTGTTGGGGTTTTCATCTGGCGCACCAGAACCAAGTCACGCCTCCGCAAAGCAAAGGCCCCTCGTTATCAACGGTTTCTGAAAAAGGTTGCTCTCCTCGATGTGAAACAGAAGGTCTCACACCTCGGATCCTACTTCAAGGATCGTGCTGCTTATAGCCCTTTATTAGCACCAACAACTCCAACAACGCCGCCGGACGCTGCTGCCTCAAATCATGTGATTGACGTCCCTCTTCAAAATTGGGTTGACGTTGCTCGCATTGTGAGAGAGAAGGACTTGAATGCACTGCGAGCACTCCGTGGTGTTGATGGTGTTTCAAGACTTCTCAGGTCTCACTCTGAGGATCATGCTGATGATGGTGGTCAAGTTCCACAACTATCATTGAAGACTACCAAGCCTGTTTTCTTTCACTTCTTGTTGAAGGCTTGCAATCAGTACACAATCTTTTTTCTCCTAGGCTCAGCTGGGTTGCAATTTGCTATTGAATTTATGAAGCAAGGAGTCAAACAAGGGTGGCATGATGGTGTTGCCATACTTGTTACTGTGTTCCTACTCGTTGCTTTTCCTTCAGTTGGGAACTATCTCAAGGAGAGGAAGATGGTGAAGCAGTTGATGGAGAGGAACAAATTTCTGGTGGATGTTGAAAGAAATGGAGAATCTAGAGCGGTTATGATATCTCATGTTTTGGTGGGTGATATAGTTCATTTGAAGAAGGGAGACCATGCTCCTGCTGATGGCTTGTTCATAGATCATGGTGTGGAACTGGTCTTGGATGAGGTATTGAGCCCCAAAATTGATTTTGAAAACAACCCATTTGTGTTGTCTGGTTCAAAAGTTATTCAGGGCCAGGGTCGAATGATTGTGACATCTGTCGGTGTCAATACTGCTTTTGCAGAGATGCTGAGCTTGGTGACTGAGGATCATAATCCAAATAGGAAGACTCTGTTACAAGCTTTGATGGACAAACCATATATTTATATGGAGTATCTTGCGTGTTGTGTTTCAATTCTGATTGCTGTTGTGGTGCTAATACGGTTACTAGTATTCAGGAAGCATGACAACTACAATGACAGGCCAGACCTGAAAGGGGAAGTTTCAGTGAACTTGCTGATGAAGATTTTCGAGAAAATATACTTGAAAACTCAAGGAAGGGTCTCCACTTTAGCAAGTGTTCTTGCTACTGTGGTAATTGGGATACAACATGGAATGCCCTTTGTGATTTCCCTTGCACTTTGTCAATGGAAGGAGAAGGTGGTCCAAAATGGGGCAGATCCTCGGAATCTTTCAGCTTGTGTCACCATGGGACTCATAACAGTCATCTGCATTGAAACAACTAGTGAGAAAATGTGCAACGAGATGGAGGTTATGGACTTTTGGATAGGTGATAAGGATTTAAGCAATGAAGTGGAGTCTGATCAAACTGAACAATTTGCTCTCAAAGGACTACGTCAAGAGATTTCTGCTACCCGAGCAAATGATTTGTTCATTTCTTCGATCAAGACCAGATGGGGTGTTACTGACATGGAGGTATTGGATCAGACACTAATAACTCTGGAAGAGAGAAAATTGAGTTCTGGCATCTTAATGAGGAAAACAGAGAGCAGTGCACAAGATATGCAGCTGCATATGAGTGGAGATGCATCAACAATCTTAGACATGTGTTCACATTATAATGATAAGACTGGGGAAAGTCGTATTCTGGAAATTCAGAAGAGAGAGTTTAAGCAGGTCATTAGGAACATGGAGGAGAAGGGTCTTAGGCCGATTGCATATGCTTACAAGAAAATAAATGATCAAGAATTTACAGAAAATGGGTTGAAATTGTTGGCACTGGTGGGAGTCAGATGCCCATATCAAGAAGAGCTTAAAATTGCAGTGGAAGCTTTTAGAAAGGCCGGAATAAGAATCAAGCTGGTATCCGAGGATGATCTCTCAATAGTGACTGAGCGAGCTTCTGATCTTGGAATCTTTAACCCTGGTAGTGGAGATATGGAGATTGCAGGCGAAGCTTTCAGAAGACTCAACTCCATGGAAAGACTAAATAAGGTGGATTTGATATCTGTGATGGGAAGTTCTCTCCCAAGAGACAAGCTTCTCATGGTGGAGCGCTTAAAGAAAAACGGTCACATAGTAGCGTTCTATGGAGGGTTAACCATAAACGATACTAGGACTTTGAAAGAAGCTGATGTTGGAATCACAGAGGATATATGGAGCACTGAGATGGCCAGAGACAATGCTGATATTAAAGTCAAGGATGGCTGCTTATGGTTCCTCAACTTGAAGTCTGGGAAATGTGCTTACCACAACATTCAGAACTTCTGTCAAATTCAGCTCACGGCTTGCATATCCGGGTTGCTAGTGACGTTAGTAGCAACGATGCATTCAGGGGACTCTCCATTGACCGCAATTCACTTGATTTGGGTGAACTTGAAAATGTGCCTTCTAGGCGGCCTAATGATGGTAATGGAATTAAATGGCCAGGAGCTACTTGATCAAAAACCAGCGCTAAGGACTCAGCCACTTATAACCAAAGCCATCTGGAGAAACATAGCCATTCAAGTTTCATACCAGGCTTCCGTGTTGCTGGTATTACATTTCATGGGAACTGCATTACCGAGCTTGAACCAAGGCATCCTTGGTACCATGGTTTTCAATATTTACACCTTGTGTCAAGTTCTTAATCTGTTCAGGGCCATAGACTTGGTGAAAAAGGAAGTGCTAAAGGTTGTGCTTCACAGCTATTGGTTTTTGATGGCTTTGGGGGCTGTTTTGGTCATGCAGGTGGTGATTGTTGAGTTTGGGAAAGGACTAGCCAGTGGTGTGAGGTTGAATGCAATGCAATGGGCAATCTGCTTTCTTCTTGCTGCTCTTTCATGGGTGTTTAATTGGGCCATGAATCGGTTCTTTTCTCGCATTCGAAGAGCAAATTTGGTATTGATGGTGAGTTCACCTATGCGGATGTCAAATAGCAGAGGTAGATTTTATATTTTTTCTGCTTGGTTGTTCCTCATCTTCTCTGTATCATATTGTTTCCAATCAGATCATTTGTACCACACGGATAGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C E1-E2_ATPase

Protein Analysis

1067

Amino Acids

120.0

Weight (kDa)

8.74

Isoelectric Point (pI)

32.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	257 - 349	1.1e-08	P-type ATPase actuator domain
Cation_ATPase	PF13246	575 - 664	2.2e-08	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	843 - 1012	1.8e-24	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008877)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40950 FvH4_6g40950
malus_domestica	MD09G1122600.v1.1 MD09G1122800.v1.1 MD17G1113500.v1.1
prunus_persica	Prupe.3G200900_v2.0.a1
pyrus_communis	pycom09g04700 pycom09g04710 pycom17g10510
rosa_chinensis	RchiOBHm_Chr2g0156561 RchiOBHm_Chr2g0156581
rosa_laevigata	RLG00000020921
rosa_multiflora	Rmu_sc0011512.1_g000006
rosa_roxburghii	Rroxscaffold_2G00092940
rosa_rugosa	Rorug02G0454300
rosa_samantha	Rh2AG520100 Rh2BG532600 Rh2BG533100 Rh2CG505100 Rh2DG541500 Rh2DG541900
rosa_wichuraiana	Rw2G042880 Rw2G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1806, 2660
AarI	CACCTGC	1 cut(s)	2905
AatII	GACGTC	1 cut(s)	363
Acc36I	ACCTGC	2 cut(s)	1852, 2905
Acc65I	GGTACC	1 cut(s)	2774
AccB1I	GGYRCC	1 cut(s)	2774
AccBSI	CCGCTC	1 cut(s)	800
AciI	CCGC	7 cut(s)	118, 181, 326, 800, 2538, 2583, 3091
AclWI	GGATC	7 cut(s)	258, 271, 285, 486, 1062, 1400, 1677
AcoI	YGGCCR	2 cut(s)	2358, 2611
AcsI	RAATTY	6 cut(s)	620, 761, 1839, 1934, 2460, 3061
AcuI	CTGAAG	2 cut(s)	54, 687
AcyI	GRCGYC	1 cut(s)	360
AdeI	CACNNNGTG	2 cut(s)	434, 2802
AfaI	GTAC	4 cut(s)	13, 572, 2776, 3185
AfeI	AGCGCT	2 cut(s)	2243, 2640
AfiI	CCNNNNNNNGG	7 cut(s)	707, 869, 1222, 1399, 2117, 2593, 3033
AflIII	ACRYGT	1 cut(s)	1791
AhlI	ACTAGT	2 cut(s)	1174, 1469
AjnI	CCWGG	4 cut(s)	981, 2110, 2613, 2703
AjuI	GAANNNNNNNTTGG	4 cut(s)	521, 553, 2307, 2339
Alw21I	GWGCWC	3 cut(s)	430, 1744, 2351
Alw26I	GTCTC	6 cut(s)	256, 471, 846, 1294, 2214, 2358
Alw44I	GTGCAC	1 cut(s)	1740
AlwI	GGATC	7 cut(s)	258, 271, 285, 486, 1062, 1400, 1677
Ama87I	CYCGRG	1 cut(s)	1602
Aor51HI	AGCGCT	2 cut(s)	2243, 2640
ApaLI	GTGCAC	1 cut(s)	1740
ApeKI	GCWGC	7 cut(s)	284, 335, 338, 1753, 1756, 2394, 2999
ApoI	RAATTY	6 cut(s)	620, 761, 1839, 1934, 2460, 3061
Asp700I	GAANNNNTTC	2 cut(s)	1253, 3035
Asp718I	GGTACC	1 cut(s)	2774
AspA2I	CCTAGG	1 cut(s)	589
AspLEI	GCGC	3 cut(s)	159, 2244, 2641
AspS9I	GGNCC	5 cut(s)	193, 979, 1390, 2826, 3024
AsuC2I	CCSGG	1 cut(s)	2485
AsuII	TTCGAA	1 cut(s)	3052
AvaI	CYCGRG	1 cut(s)	1602
AvaII	GGWCC	1 cut(s)	1390
AvrII	CCTAGG	1 cut(s)	589
BaeGI	GKGCMC	1 cut(s)	1744
BaeI	ACNNNNGTAYC	2 cut(s)	2766, 2799
BalI	TGGCCA	2 cut(s)	2360, 2613
BamHI	GGATCC	1 cut(s)	263
BanI	GGYRCC	1 cut(s)	2774
BanII	GRGCYC	1 cut(s)	923
BbsI	GAAGAC	3 cut(s)	527, 1079, 2158
Bbv12I	GWGCWC	3 cut(s)	430, 1744, 2351
BbvI	GCAGC	7 cut(s)	271, 322, 325, 1743, 1765, 2381, 2986
BceAI	ACGGC	1 cut(s)	2487
BciT130I	CCWGG	4 cut(s)	983, 2112, 2615, 2705
BciVI	GTATCC	2 cut(s)	1323, 2062
BclI	TGATCA	4 cut(s)	22, 1549, 1927, 2626
BcnI	CCSGG	1 cut(s)	2485
BcoDI	GTCTC	6 cut(s)	256, 471, 846, 1294, 2214, 2358
BcuI	ACTAGT	2 cut(s)	1174, 1469
BfaI	CTAG	8 cut(s)	590, 795, 1175, 1470, 2301, 2492, 2579, 2945
BfoI	RGCGCY	2 cut(s)	2245, 2642
BfuAI	ACCTGC	2 cut(s)	1852, 2905
BfuI	GTATCC	2 cut(s)	1323, 2062
BglI	GCCNNNNNGGC	1 cut(s)	2580
BlnI	CCTAGG	1 cut(s)	589
BlpI	GCTNAGC	2 cut(s)	595, 1037
Bme1390I	CCNGG	5 cut(s)	983, 2112, 2485, 2615, 2705
Bme18I	GGWCC	1 cut(s)	1390
BmeT110I	CYCGRG	1 cut(s)	1602
BmgT120I	GGNCC	5 cut(s)	193, 979, 1390, 2826, 3024
BmiI	GGNNCC	4 cut(s)	195, 265, 2405, 2776
BmrFI	CCNGG	5 cut(s)	983, 2112, 2485, 2615, 2705
BmrI	ACTGGG	1 cut(s)	1827
BmsI	GCATC	7 cut(s)	1023, 1722, 1762, 1784, 1970, 2502, 2775
BmuI	ACTGGG	1 cut(s)	1827
BoxI	GACNNNNGTC	1 cut(s)	1004
BpiI	GAAGAC	3 cut(s)	527, 1079, 2158
BpmI	CTGGAG	1 cut(s)	2695
Bpu10I	CCTNAGC	1 cut(s)	2642
Bpu1102I	GCTNAGC	2 cut(s)	595, 1037
Bpu14I	TTCGAA	1 cut(s)	3052
BpuEI	CTTGAG	2 cut(s)	704, 1263
BpuMI	CCSGG	1 cut(s)	2485
BsaBI	GATNNNNATC	1 cut(s)	1059
BsaHI	GRCGYC	1 cut(s)	360
BsaI	GGTCTC	4 cut(s)	256, 471, 846, 1294
Bsc4I	CCNNNNNNNGG	7 cut(s)	707, 869, 1222, 1399, 2117, 2593, 3033
Bse1I	ACTGG	4 cut(s)	903, 1822, 1971, 2949
Bse3DI	GCAATG	4 cut(s)	132, 1540, 2976, 2981
Bse8I	GATNNNNATC	1 cut(s)	1059
BseBI	CCWGG	4 cut(s)	983, 2112, 2615, 2705
BseGI	GGATG	6 cut(s)	778, 913, 2065, 2395, 2766, 3099
BseJI	GATNNNNATC	1 cut(s)	1059
BseLI	CCNNNNNNNGG	7 cut(s)	707, 869, 1222, 1399, 2117, 2593, 3033
BseMI	GCAATG	4 cut(s)	132, 1540, 2976, 2981
BseMII	CTCAG	8 cut(s)	465, 475, 609, 1028, 1041, 2070, 2343, 2663
BseNI	ACTGG	4 cut(s)	903, 1822, 1971, 2949
BseRI	GAGGAG	2 cut(s)	221, 1895
BseSI	GKGCMC	1 cut(s)	1744
BseXI	GCAGC	7 cut(s)	271, 322, 325, 1743, 1765, 2381, 2986
BseYI	CCCAGC	1 cut(s)	599
BshNI	GGYRCC	1 cut(s)	2774
BsiHKAI	GWGCWC	3 cut(s)	430, 1744, 2351
BsiHKCI	CYCGRG	1 cut(s)	1602
BsiSI	CCGG	3 cut(s)	329, 2031, 2484
BslFI	GGGAC	3 cut(s)	347, 1452, 2537
BslI	CCNNNNNNNGG	7 cut(s)	707, 869, 1222, 1399, 2117, 2593, 3033
BsmAI	GTCTC	6 cut(s)	256, 471, 846, 1294, 2214, 2358
BsmFI	GGGAC	3 cut(s)	347, 1452, 2537
BsmI	GAATGC	5 cut(s)	421, 1347, 2515, 2971, 3048
Bso31I	GGTCTC	4 cut(s)	256, 471, 846, 1294
BsoBI	CYCGRG	1 cut(s)	1602
Bsp119I	TTCGAA	1 cut(s)	3052
Bsp1286I	GDGCHC	4 cut(s)	430, 923, 1744, 2351
Bsp1720I	GCTNAGC	2 cut(s)	595, 1037
Bsp19I	CCATGG	3 cut(s)	1434, 2163, 2778
BspACI	CCGC	7 cut(s)	118, 181, 326, 800, 2538, 2583, 3091
BspCNI	CTCAG	8 cut(s)	466, 474, 608, 1029, 1042, 2071, 2344, 2662
BspLI	GGNNCC	4 cut(s)	195, 265, 2405, 2776
BspMI	ACCTGC	2 cut(s)	1852, 2905
BspPI	GGATC	7 cut(s)	258, 271, 285, 486, 1062, 1400, 1677
BspQI	GCTCTTC	2 cut(s)	1989, 3049
BspT104I	TTCGAA	1 cut(s)	3052
BspT107I	GGYRCC	1 cut(s)	2774
BspTNI	GGTCTC	4 cut(s)	256, 471, 846, 1294
BsrBI	CCGCTC	1 cut(s)	800
BsrDI	GCAATG	4 cut(s)	132, 1540, 2976, 2981
BsrI	ACTGG	4 cut(s)	903, 1822, 1971, 2949
BssNI	GRCGYC	1 cut(s)	360
BssT1I	CCWWGG	6 cut(s)	589, 1434, 2163, 2761, 2770, 2778
Bst2UI	CCWGG	4 cut(s)	983, 2112, 2615, 2705
Bst4CI	ACNGT	8 cut(s)	11, 79, 210, 679, 1170, 1318, 1450, 2258
Bst6I	CTCTTC	5 cut(s)	372, 1686, 1841, 1989, 3049
BstACI	GRCGYC	1 cut(s)	360
BstBI	TTCGAA	1 cut(s)	3052
BstC8I	GCNNGC	7 cut(s)	110, 391, 426, 562, 2085, 2137, 2476
BstDSI	CCRYGG	4 cut(s)	433, 1434, 2163, 2778
BstF5I	GGATG	6 cut(s)	778, 913, 2065, 2395, 2766, 3099
BstH2I	RGCGCY	2 cut(s)	2245, 2642
BstHHI	GCGC	3 cut(s)	159, 2244, 2641
BstMAI	GTCTC	6 cut(s)	256, 471, 846, 1294, 2214, 2358
BstMWI	GCNNNNNNNGC	8 cut(s)	109, 114, 425, 1604, 2015, 2141, 2580, 2897
BstNI	CCWGG	4 cut(s)	983, 2112, 2615, 2705
BstNSI	RCATGY	1 cut(s)	1795
BstPAI	GACNNNNGTC	1 cut(s)	1004
BstSCI	CCNGG	5 cut(s)	981, 2110, 2483, 2613, 2703
BstSLI	GKGCMC	1 cut(s)	1744
BstV1I	GCAGC	7 cut(s)	271, 322, 325, 1743, 1765, 2381, 2986
BstV2I	GAAGAC	3 cut(s)	527, 1079, 2158
BstX2I	RGATCY	2 cut(s)	263, 1405
BstXI	CCANNNNNNTGG	1 cut(s)	2836
BstYI	RGATCY	2 cut(s)	263, 1405
BsuI	GTATCC	2 cut(s)	1323, 2062
BtgI	CCRYGG	4 cut(s)	433, 1434, 2163, 2778
BtsCI	GGATG	6 cut(s)	778, 913, 2065, 2395, 2766, 3099
BtsI	GCAGTG	3 cut(s)	419, 1744, 2016
BtsIMutI	CAGTG	7 cut(s)	419, 1239, 1744, 1964, 2016, 2349, 2956
BveI	ACCTGC	2 cut(s)	1852, 2905
Cac8I	GCNNGC	7 cut(s)	110, 391, 426, 562, 2085, 2137, 2476
CfoI	GCGC	3 cut(s)	159, 2244, 2641
Cfr13I	GGNCC	5 cut(s)	193, 979, 1390, 2826, 3024
CseI	GACGC	1 cut(s)	341
Csp6I	GTAC	4 cut(s)	12, 571, 2775, 3184
CviQI	GTAC	4 cut(s)	12, 571, 2775, 3184
DraIII	CACNNNGTG	2 cut(s)	434, 2802
EaeI	YGGCCR	2 cut(s)	2358, 2611
Eam1104I	CTCTTC	5 cut(s)	372, 1686, 1841, 1989, 3049
EarI	CTCTTC	5 cut(s)	372, 1686, 1841, 1989, 3049
Eco130I	CCWWGG	6 cut(s)	589, 1434, 2163, 2761, 2770, 2778
Eco24I	GRGCYC	1 cut(s)	923
Eco31I	GGTCTC	4 cut(s)	256, 471, 846, 1294
Eco32I	GATATC	2 cut(s)	810, 2193
Eco47I	GGWCC	1 cut(s)	1390
Eco47III	AGCGCT	2 cut(s)	2243, 2640
Eco57I	CTGAAG	2 cut(s)	54, 687
Eco88I	CYCGRG	1 cut(s)	1602
EcoO109I	RGGNCCY	1 cut(s)	193
EcoRII	CCWGG	4 cut(s)	981, 2110, 2613, 2703
EcoRV	GATATC	2 cut(s)	810, 2193
EcoT14I	CCWWGG	6 cut(s)	589, 1434, 2163, 2761, 2770, 2778
EcoT22I	ATGCAT	2 cut(s)	1777, 2517
EcoT38I	GRGCYC	1 cut(s)	923
ErhI	CCWWGG	6 cut(s)	589, 1434, 2163, 2761, 2770, 2778
FalI	AAGNNNNNCTT	4 cut(s)	1983, 2015, 2754, 2786
FaqI	GGGAC	3 cut(s)	347, 1452, 2537
FauNDI	CATATG	2 cut(s)	1761, 1906
FbaI	TGATCA	4 cut(s)	22, 1549, 1927, 2626
FokI	GGATG	6 cut(s)	785, 920, 2072, 2402, 2753, 3106
FriOI	GRGCYC	1 cut(s)	923
FspBI	CTAG	8 cut(s)	590, 795, 1175, 1470, 2301, 2492, 2579, 2945
GlaI	GCGC	3 cut(s)	158, 2243, 2640
GsaI	CCCAGC	1 cut(s)	603
GsuI	CTGGAG	1 cut(s)	2695
HaeII	RGCGCY	2 cut(s)	2245, 2642
HapII	CCGG	3 cut(s)	329, 2031, 2484
HgaI	GACGC	1 cut(s)	341
HhaI	GCGC	3 cut(s)	159, 2244, 2641
Hin1I	GRCGYC	1 cut(s)	360
Hin6I	GCGC	3 cut(s)	157, 2242, 2639
HinP1I	GCGC	3 cut(s)	157, 2242, 2639
HincII	GTYRAC	2 cut(s)	382, 2286
HindII	GTYRAC	2 cut(s)	382, 2286
HindIII	AAGCTT	5 cut(s)	27, 1086, 2016, 2142, 2225
HpaI	GTTAAC	1 cut(s)	2286
HpaII	CCGG	3 cut(s)	329, 2031, 2484
Hpy166II	GTNNAC	8 cut(s)	382, 573, 1237, 1742, 1799, 2286, 2560, 3083
Hpy8I	GTNNAC	8 cut(s)	382, 573, 1237, 1742, 1799, 2286, 2560, 3083
HpyCH4III	ACNGT	8 cut(s)	11, 79, 210, 679, 1170, 1318, 1450, 2258
HpyCH4IV	ACGT	4 cut(s)	360, 384, 1582, 2498
HpyF10VI	GCNNNNNNNGC	8 cut(s)	109, 114, 425, 1604, 2015, 2141, 2580, 2897
HpySE526I	ACGT	4 cut(s)	360, 384, 1582, 2498
Hsp92I	GRCGYC	1 cut(s)	360
HspAI	GCGC	3 cut(s)	157, 2242, 2639
KpnI	GGTACC	1 cut(s)	2778
Ksp22I	TGATCA	4 cut(s)	22, 1549, 1927, 2626
KspAI	GTTAAC	1 cut(s)	2286
LguI	GCTCTTC	2 cut(s)	1989, 3049
LmnI	GCTCC	4 cut(s)	865, 2239, 2346, 2617
Lsp1109I	GCAGC	7 cut(s)	271, 322, 325, 1743, 1765, 2381, 2986
LweI	GCATC	7 cut(s)	1023, 1722, 1762, 1784, 1970, 2502, 2775
MaeI	CTAG	8 cut(s)	590, 795, 1175, 1470, 2301, 2492, 2579, 2945
MaeII	ACGT	4 cut(s)	360, 384, 1582, 2498
MbiI	CCGCTC	1 cut(s)	800
MflI	RGATCY	2 cut(s)	263, 1405
MhlI	GDGCHC	4 cut(s)	430, 923, 1744, 2351
MlsI	TGGCCA	2 cut(s)	2360, 2613
MluNI	TGGCCA	2 cut(s)	2360, 2613
MlyI	GAGTC	8 cut(s)	645, 1069, 1434, 1553, 1983, 2148, 2519, 2641
MmeI	TCCRAC	2 cut(s)	341, 2305
Mox20I	TGGCCA	2 cut(s)	2360, 2613
Mph1103I	ATGCAT	2 cut(s)	1777, 2517
MroXI	GAANNNNTTC	2 cut(s)	1253, 3035
MscI	TGGCCA	2 cut(s)	2360, 2613
MslI	CAYNNNNRTG	7 cut(s)	486, 657, 1718, 1764, 1806, 2354, 2915
Msp20I	TGGCCA	2 cut(s)	2360, 2613
MspA1I	CMGCKG	3 cut(s)	120, 599, 1756
MspI	CCGG	3 cut(s)	329, 2031, 2484
MspR9I	CCNGG	5 cut(s)	983, 2112, 2485, 2615, 2705
Mva1269I	GAATGC	5 cut(s)	421, 1347, 2515, 2971, 3048
MvaI	CCWGG	4 cut(s)	983, 2112, 2615, 2705
MwoI	GCNNNNNNNGC	8 cut(s)	109, 114, 425, 1604, 2015, 2141, 2580, 2897
NciI	CCSGG	1 cut(s)	2485
NcoI	CCATGG	3 cut(s)	1434, 2163, 2778
NdeI	CATATG	2 cut(s)	1761, 1906
NlaIV	GGNNCC	4 cut(s)	195, 265, 2405, 2776
NmuCI	GTSAC	7 cut(s)	171, 997, 1045, 1429, 2074, 2258, 2494
NsiI	ATGCAT	2 cut(s)	1777, 2517
NspI	RCATGY	1 cut(s)	1795
NspV	TTCGAA	1 cut(s)	3052
PaqCI	CACCTGC	1 cut(s)	2905
PciI	ACATGT	1 cut(s)	1791
PciSI	GCTCTTC	2 cut(s)	1989, 3049
PctI	GAATGC	5 cut(s)	421, 1347, 2515, 2971, 3048
PdmI	GAANNNNTTC	2 cut(s)	1253, 3035
PfeI	GAWTC	6 cut(s)	791, 1414, 2040, 2100, 2328, 3031
PflFI	GACNNNGTC	1 cut(s)	1581
PleI	GAGTC	8 cut(s)	644, 1069, 1434, 1552, 1982, 2148, 2519, 2641
PpsI	GAGTC	8 cut(s)	644, 1069, 1434, 1552, 1982, 2148, 2519, 2641
PscI	ACATGT	1 cut(s)	1791
PshAI	GACNNNNGTC	1 cut(s)	1004
PsiI	TTATAA	2 cut(s)	1806, 2660
Psp6I	CCWGG	4 cut(s)	981, 2110, 2613, 2703
PspFI	CCCAGC	1 cut(s)	599
PspGI	CCWGG	4 cut(s)	981, 2110, 2613, 2703
PspN4I	GGNNCC	4 cut(s)	195, 265, 2405, 2776
PspPI	GGNCC	5 cut(s)	193, 979, 1390, 2826, 3024
PsuI	RGATCY	2 cut(s)	263, 1405
PsyI	GACNNNGTC	1 cut(s)	1581
PvuII	CAGCTG	2 cut(s)	599, 1756
RsaI	GTAC	4 cut(s)	13, 572, 2776, 3185
RsaNI	GTAC	4 cut(s)	12, 571, 2775, 3184
RseI	CAYNNNNRTG	7 cut(s)	486, 657, 1718, 1764, 1806, 2354, 2915
SapI	GCTCTTC	2 cut(s)	1989, 3049
Sau96I	GGNCC	5 cut(s)	193, 979, 1390, 2826, 3024
SchI	GAGTC	8 cut(s)	645, 1069, 1434, 1553, 1983, 2148, 2519, 2641
ScrFI	CCNGG	5 cut(s)	983, 2112, 2485, 2615, 2705
SduI	GDGCHC	4 cut(s)	430, 923, 1744, 2351
SfaNI	GCATC	7 cut(s)	1023, 1722, 1762, 1784, 1970, 2502, 2775
SfuI	TTCGAA	1 cut(s)	3052
SinI	GGWCC	1 cut(s)	1390
SmiMI	CAYNNNNRTG	7 cut(s)	486, 657, 1718, 1764, 1806, 2354, 2915
SmlI	CTYRAG	2 cut(s)	719, 1278
SmoI	CTYRAG	2 cut(s)	719, 1278
SpeI	ACTAGT	2 cut(s)	1174, 1469
SsiI	CCGC	7 cut(s)	118, 181, 326, 800, 2538, 2583, 3091
SspI	AATATT	1 cut(s)	2791
SspMI	CTAG	8 cut(s)	590, 795, 1175, 1470, 2301, 2492, 2579, 2945
StyD4I	CCNGG	5 cut(s)	981, 2110, 2483, 2613, 2703
StyI	CCWWGG	6 cut(s)	589, 1434, 2163, 2761, 2770, 2778
TaaI	ACNGT	8 cut(s)	11, 79, 210, 679, 1170, 1318, 1450, 2258
TaiI	ACGT	4 cut(s)	363, 387, 1585, 2501
TaqI	TCGA	6 cut(s)	50, 234, 988, 1257, 1628, 3052
TatI	WGTACW	1 cut(s)	570
TauI	GCSGC	3 cut(s)	120, 328, 2586
TfiI	GAWTC	6 cut(s)	791, 1414, 2040, 2100, 2328, 3031
TscAI	CASTG	7 cut(s)	426, 1239, 1744, 1971, 2016, 2356, 2956
TseFI	GTSAC	7 cut(s)	171, 997, 1045, 1429, 2074, 2258, 2494
TseI	GCWGC	7 cut(s)	284, 335, 338, 1753, 1756, 2394, 2999
Tsp45I	GTSAC	7 cut(s)	171, 997, 1045, 1429, 2074, 2258, 2494
TspGWI	ACGGA	2 cut(s)	422, 2701
TspRI	CASTG	7 cut(s)	426, 1239, 1744, 1971, 2016, 2356, 2956
Tth111I	GACNNNGTC	1 cut(s)	1581
VneI	GTGCAC	1 cut(s)	1740
VpaK11BI	GGWCC	1 cut(s)	1390
XapI	RAATTY	6 cut(s)	620, 761, 1839, 1934, 2460, 3061
XbaI	TCTAGA	1 cut(s)	794
XceI	RCATGY	1 cut(s)	1795
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2768
XmaJI	CCTAGG	1 cut(s)	589
XmnI	GAANNNNTTC	2 cut(s)	1253, 3035
XspI	CTAG	8 cut(s)	590, 795, 1175, 1470, 2301, 2492, 2579, 2945
ZraI	GACGTC	1 cut(s)	361
Zsp2I	ATGCAT	2 cut(s)	1777, 2517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.