FvH4_6g43020

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002246
Physical Location & Seq
Forward (+)
33355771 .. 33357124
1354 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43020.t1

Sequence Viewer

Length: 726 bp
ATGGGGAGCATGATCAAAGCAGCGCTGGGAGATGCAATTTTGACTTCCATGTGGGTTTTCAGTGCCCCATCCATGGGGGTGTTCACAACCATCATAGCTTCGTTTCTTGGAGTCCAAGCAAGGTCCTTGACAGGCCTTTTCATCACCACCATACTCGCCACAACTTTCGTCTTGACTTTCAGCTTGATTGGCAAGCTCTTGGGTGGTGCCAGCTTCAACCCCTCCACCACAGCCAGCTTCTACGCCGCCGGCCTCAGCCCCGGAACCTCCCTTATCTCGATGGCAGTCAGGTTCCCGGCTCAAGCGGCCGGCGGCGTAGGCGGCGCCAAGGCCATCCTGCAGGTGATGCCTAGCAAGTTCAAGCACTTTGTCAAAGGGCCTTCTCTGAAAGTTGATGTGAAAAGTGGTGCTGTTGCTGAAGGGTTGTTCACTTTTTCCCTATGCTTTGCTTTGCTTTGGATTATACTTAGAGGTCCTAAGAATCCAATTGTGCAATTATGGTTGCTTTCTGTGGCTACTGTGGGATTGGTTGTTGTGGGAGGTGGGTACACAGGGCCTTCTATGAACCCTGCTAATGCTTTTGGCTGGGCTTATGTCAATAACTGGCACAACACTTGGGAGCTCTTCTTTGTTTATTGGATTGGTCCTTTAGTTGGAGCAATTTTGGCTGCTAGGGTCTTCAAGGTTTTGTTTCCTCCACCAGAACCAAAGGAGAAGAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

242

Amino Acids

25.44

Weight (kDa)

10.05

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 6 - 226 5.1e-16 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
Acc36I ACCTGC 1 cut(s) 331
AccB1I GGYRCC 2 cut(s) 206, 323
AciI CCGC 4 cut(s) 246, 305, 312, 321
AcoI YGGCCR 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 548
AfeI AGCGCT 1 cut(s) 24
AfiI CCNNNNNNNGG 3 cut(s) 73, 74, 653
AgsI TTSAA 3 cut(s) 217, 361, 682
AluBI AGCT 6 cut(s) 98, 183, 196, 213, 237, 622
AluI AGCT 6 cut(s) 98, 183, 196, 213, 237, 622
Alw21I GWGCWC 1 cut(s) 624
Aor51HI AGCGCT 1 cut(s) 24
AoxI GGCC 6 cut(s) 133, 250, 306, 330, 377, 554
ApeKI GCWGC 2 cut(s) 20, 668
AspLEI GCGC 2 cut(s) 25, 326
AspS9I GGNCC 5 cut(s) 123, 377, 473, 554, 644
AsuC2I CCSGG 2 cut(s) 261, 296
AsuHPI GGTGA 2 cut(s) 136, 355
AvaII GGWCC 3 cut(s) 123, 473, 644
BaeGI GKGCMC 1 cut(s) 67
BanI GGYRCC 2 cut(s) 206, 323
BanII GRGCYC 1 cut(s) 624
BbsI GAAGAC 1 cut(s) 670
Bbv12I GWGCWC 1 cut(s) 624
BbvCI CCTCAGC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 32, 655
BccI CCATC 4 cut(s) 76, 98, 274, 341
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 2 cut(s) 261, 296
BfaI CTAG 2 cut(s) 351, 672
BfmI CTRYAG 1 cut(s) 338
BfoI RGCGCY 2 cut(s) 26, 327
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 6 cut(s) 21, 246, 306, 313, 322, 669
BlsI GCNGC 6 cut(s) 22, 247, 307, 314, 323, 670
Bme1390I CCNGG 2 cut(s) 261, 296
Bme18I GGWCC 3 cut(s) 123, 473, 644
BmgT120I GGNCC 5 cut(s) 123, 377, 473, 554, 644
BmiI GGNNCC 4 cut(s) 208, 265, 293, 325
BmrFI CCNGG 2 cut(s) 261, 296
BmsI GCATC 2 cut(s) 22, 336
BpiI GAAGAC 1 cut(s) 670
Bpu10I CCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 261, 296
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 72, 259, 327
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 74, 653
Bse118I RCCGGY 2 cut(s) 248, 308
Bse1I ACTGG 1 cut(s) 608
BseDI CCNNGG 3 cut(s) 72, 259, 327
BseGI GGATG 2 cut(s) 68, 333
BseLI CCNNNNNNNGG 3 cut(s) 73, 74, 653
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 608
BseSI GKGCMC 1 cut(s) 67
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 2 cut(s) 32, 655
BseYI CCCAGC 2 cut(s) 25, 585
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
BshNI GGYRCC 2 cut(s) 206, 323
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 624
BsiSI CCGG 4 cut(s) 249, 261, 296, 309
BslI CCNNNNNNNGG 3 cut(s) 73, 74, 653
BsnI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
Bsp1286I GDGCHC 2 cut(s) 67, 624
Bsp143I GATC 1 cut(s) 12
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 4 cut(s) 246, 305, 312, 321
BspANI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
BspCNI CTCAG 1 cut(s) 267
BspLI GGNNCC 4 cut(s) 208, 265, 293, 325
BspMAI CTGCAG 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 331
BspQI GCTCTTC 1 cut(s) 629
BspT107I GGYRCC 2 cut(s) 206, 323
BsrFI RCCGGY 2 cut(s) 248, 308
BsrI ACTGG 1 cut(s) 608
BssAI RCCGGY 2 cut(s) 248, 308
BssECI CCNNGG 3 cut(s) 72, 259, 327
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 72, 327
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 5 cut(s) 194, 211, 235, 250, 310
BstDEI CTNAG 3 cut(s) 254, 467, 477
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 68, 333
BstH2I RGCGCY 2 cut(s) 26, 327
BstHHI GCGC 2 cut(s) 25, 326
BstKTI GATC 1 cut(s) 15
BstMBI GATC 1 cut(s) 12
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 6 cut(s) 189, 305, 318, 321, 346, 665
BstSCI CCNGG 2 cut(s) 259, 294
BstSFI CTRYAG 1 cut(s) 338
BstSLI GKGCMC 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 32, 655
BstV2I GAAGAC 1 cut(s) 670
BstZI CGGCCG 1 cut(s) 306
BsuRI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 68, 333
BtsIMutI CAGTG 1 cut(s) 67
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 5 cut(s) 194, 211, 235, 250, 310
CfoI GCGC 2 cut(s) 25, 326
Cfr10I RCCGGY 2 cut(s) 248, 308
Cfr13I GGNCC 5 cut(s) 123, 377, 473, 554, 644
Csp6I GTAC 1 cut(s) 547
CviAII CATG 3 cut(s) 10, 49, 73
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 3 cut(s) 254, 467, 477
DinI GGCGCC 1 cut(s) 325
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
EaeI YGGCCR 1 cut(s) 306
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Ecl136II GAGCTC 1 cut(s) 622
EclXI CGGCCG 1 cut(s) 306
Eco130I CCWWGG 2 cut(s) 72, 327
Eco147I AGGCCT 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 624
Eco47I GGWCC 3 cut(s) 123, 473, 644
Eco47III AGCGCT 1 cut(s) 24
Eco52I CGGCCG 1 cut(s) 306
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 438
EcoICRI GAGCTC 1 cut(s) 622
EcoO109I RGGNCCY 4 cut(s) 123, 377, 473, 554
EcoT14I CCWWGG 2 cut(s) 72, 327
EcoT38I GRGCYC 1 cut(s) 624
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 72, 327
FaeI CATG 3 cut(s) 13, 52, 76
FatI CATG 3 cut(s) 9, 48, 72
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 6 cut(s) 21, 246, 306, 313, 322, 669
FokI GGATG 2 cut(s) 55, 320
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 6 cut(s) 21, 246, 306, 313, 322, 669
FspBI CTAG 2 cut(s) 351, 672
GlaI GCGC 2 cut(s) 24, 325
GluI GCNGC 6 cut(s) 21, 246, 306, 313, 322, 669
GsaI CCCAGC 2 cut(s) 29, 589
HaeII RGCGCY 2 cut(s) 26, 327
HaeIII GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
HapII CCGG 4 cut(s) 249, 261, 296, 309
HhaI GCGC 2 cut(s) 25, 326
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 3 cut(s) 13, 52, 76
Hin6I GCGC 2 cut(s) 23, 324
HinP1I GCGC 2 cut(s) 23, 324
HinfI GANTC 2 cut(s) 111, 481
HpaII CCGG 4 cut(s) 249, 261, 296, 309
HphI GGTGA 2 cut(s) 136, 355
Hpy166II GTNNAC 3 cut(s) 84, 429, 549
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 2 cut(s) 172, 277
Hpy8I GTNNAC 3 cut(s) 84, 429, 549
HpyAV CCTTC 3 cut(s) 390, 413, 567
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 3 cut(s) 35, 340, 493
HpyF10VI GCNNNNNNNGC 6 cut(s) 189, 305, 318, 321, 346, 665
HpyF3I CTNAG 3 cut(s) 254, 467, 477
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 3 cut(s) 13, 52, 76
HspAI GCGC 2 cut(s) 23, 324
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 2 cut(s) 248, 308
KroNI GCCGGC 2 cut(s) 250, 310
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 1 cut(s) 12
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 3 cut(s) 6, 619, 656
Lsp1109I GCAGC 2 cut(s) 32, 655
LweI GCATC 2 cut(s) 22, 336
MaeI CTAG 2 cut(s) 351, 672
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MboII GAAGA 2 cut(s) 616, 670
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 2 cut(s) 67, 624
MluCI AATT 4 cut(s) 36, 486, 494, 660
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 6 cut(s) 232, 263, 277, 464, 533, 705
MroNI GCCGGC 2 cut(s) 248, 308
MslI CAYNNNNRTG 1 cut(s) 77
MspI CCGG 4 cut(s) 249, 261, 296, 309
MspR9I CCNGG 2 cut(s) 261, 296
MunI CAATTG 1 cut(s) 486
MwoI GCNNNNNNNGC 6 cut(s) 189, 305, 318, 321, 346, 665
NaeI GCCGGC 2 cut(s) 250, 310
NarI GGCGCC 1 cut(s) 324
NciI CCSGG 2 cut(s) 261, 296
NcoI CCATGG 1 cut(s) 72
NdeII GATC 1 cut(s) 12
NgoMIV GCCGGC 2 cut(s) 248, 308
NlaIII CATG 3 cut(s) 13, 52, 76
NlaIV GGNNCC 4 cut(s) 208, 265, 293, 325
PaqCI CACCTGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 629
PdiI GCCGGC 2 cut(s) 250, 310
PfeI GAWTC 1 cut(s) 481
PkrI GCNGC 6 cut(s) 22, 247, 307, 314, 323, 670
PleI GAGTC 1 cut(s) 119
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 2 cut(s) 123, 473
Psp124BI GAGCTC 1 cut(s) 624
Psp5II RGGWCCY 2 cut(s) 123, 473
PspFI CCCAGC 2 cut(s) 25, 585
PspN4I GGNNCC 4 cut(s) 208, 265, 293, 325
PspPI GGNCC 5 cut(s) 123, 377, 473, 554, 644
PspPPI RGGWCCY 2 cut(s) 123, 473
PstI CTGCAG 1 cut(s) 342
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 1 cut(s) 77
SacI GAGCTC 1 cut(s) 624
SapI GCTCTTC 1 cut(s) 629
SatI GCNGC 6 cut(s) 21, 246, 306, 313, 322, 669
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 5 cut(s) 123, 377, 473, 554, 644
SbfI CCTGCAGG 1 cut(s) 342
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 261, 296
SdaI CCTGCAGG 1 cut(s) 342
SduI GDGCHC 2 cut(s) 67, 624
SfaNI GCATC 2 cut(s) 22, 336
SfcI CTRYAG 1 cut(s) 338
SfoI GGCGCC 1 cut(s) 325
SinI GGWCC 3 cut(s) 123, 473, 644
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse8387I CCTGCAGG 1 cut(s) 342
Sse9I AATT 4 cut(s) 36, 486, 494, 660
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 4 cut(s) 246, 305, 312, 321
SspDI GGCGCC 1 cut(s) 323
SspMI CTAG 2 cut(s) 351, 672
SstI GAGCTC 1 cut(s) 624
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 259, 294
StyI CCWWGG 2 cut(s) 72, 327
TaaI ACNGT 1 cut(s) 520
TaqI TCGA 1 cut(s) 278
TasI AATT 4 cut(s) 36, 486, 494, 660
TauI GCSGC 4 cut(s) 248, 308, 315, 324
TfiI GAWTC 1 cut(s) 481
TscAI CASTG 1 cut(s) 67
TseI GCWGC 2 cut(s) 20, 668
TspDTI ATGAA 2 cut(s) 130, 578
TspRI CASTG 1 cut(s) 67
VpaK11BI GGWCC 3 cut(s) 123, 473, 644
XspI CTAG 2 cut(s) 351, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.