RLG00000021131

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002246
Physical Location & Seq
Forward (+)
72175946 .. 72176677
732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021131

Sequence Viewer

Length: 732 bp
ATGAAGATAGGTAGTTTGTTCAAAGCAGCTGTAGGAGATGCAATTTTGACTTCCATGTTCGTTTTCATTGGCCCAGTCATGCGTGTTTTCACATCCATCATAGCTTCATTTCTTGGTTGCCAAGCTAGGTCCTTGGTAGGCCGTTTCATCACCACCATACATGCCACAGTTCTGGTCCTGATATTCAGCATGATTGGCAAGCTCTTGGGTGGTGCTAGCTTCAATCCCTCCACCACTGTCAGTTTGTACGCCGCCGGCCTCAACCCGGGAACTTCCCTGATCTCAATGGCAGTCCAGTTCCCGGCCCAGGCGGCAGGCGGAGCAGGTGGCGCCAAGGCCATCCTGCAAGTGATGCCTAAAAAGTATGAACATTTTGTCAAAGGGCCTTCTCTGAAAGTGGATGTGAAAAGTGGTGCTATTGCTGAAGGGTTGTTCACTTTTTCTCTTTGCTTTTCTCTGCTTTGGGTTATGCTTAGGGGTCCTAAGAATCTTTTTCTGCAAATATGGTTGGTTAAAGTGGCTACTGTGGGATTGGCTATTGTGGGAAGTGGGTACACAGGGCCTTCTATGAACCCTGCTAATGCTTTTGGCTGGGCTTATGTTGATAACTGGCACAATACTTGGGAGCTGTTCTTTGTTTATTGGGTTGGTCCTTTAATTGGAGCAGCTTTGGCTGCTAGGGTCTTCAAGATTTTGTTTCCTCCACCAGAAACAAAGGAGAAGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

244

Amino Acids

25.96

Weight (kDa)

9.93

Isoelectric Point (pI)

18.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 6 - 228 4.8e-18 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 329
AciI CCGC 3 cut(s) 252, 311, 318
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 1 cut(s) 330
AfaI GTAC 2 cut(s) 248, 554
AfiI CCNNNNNNNGG 4 cut(s) 265, 301, 307, 659
AgsI TTSAA 3 cut(s) 22, 223, 688
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 7 cut(s) 29, 104, 125, 202, 219, 628, 668
AluI AGCT 7 cut(s) 29, 104, 125, 202, 219, 628, 668
Ama87I CYCGRG 1 cut(s) 265
AoxI GGCC 7 cut(s) 70, 139, 256, 303, 336, 383, 560
ApeKI GCWGC 3 cut(s) 26, 665, 674
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 8 cut(s) 71, 129, 175, 304, 383, 479, 560, 650
AsuC2I CCSGG 3 cut(s) 266, 267, 302
AsuHPI GGTGA 1 cut(s) 142
AsuNHI GCTAGC 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 265
AvaII GGWCC 4 cut(s) 129, 175, 479, 650
BanI GGYRCC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 676
BbvI GCAGC 3 cut(s) 38, 661, 677
BccI CCATC 2 cut(s) 104, 347
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 308
BcnI CCSGG 3 cut(s) 266, 267, 302
BfaI CTAG 3 cut(s) 126, 216, 678
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 314
BglI GCCNNNNNGGC 1 cut(s) 311
BisI GCNGC 5 cut(s) 27, 252, 312, 666, 675
BlsI GCNGC 5 cut(s) 28, 253, 313, 667, 676
Bme1390I CCNGG 4 cut(s) 266, 267, 302, 308
Bme18I GGWCC 4 cut(s) 129, 175, 479, 650
BmeT110I CYCGRG 1 cut(s) 265
BmgT120I GGNCC 8 cut(s) 71, 129, 175, 304, 383, 479, 560, 650
BmiI GGNNCC 2 cut(s) 331, 480
BmrFI CCNGG 4 cut(s) 266, 267, 302, 308
BmrI ACTGGG 1 cut(s) 68
BmsI GCATC 2 cut(s) 28, 342
BmtI GCTAGC 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 676
Bpu10I CCTNAGC 1 cut(s) 473
BpuMI CCSGG 3 cut(s) 266, 267, 302
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 4 cut(s) 132, 265, 306, 333
Bsc4I CCNNNNNNNGG 4 cut(s) 265, 301, 307, 659
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 3 cut(s) 74, 295, 614
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 4 cut(s) 132, 265, 306, 333
BseGI GGATG 3 cut(s) 92, 339, 406
BseLI CCNNNNNNNGG 4 cut(s) 265, 301, 307, 659
BseNI ACTGG 3 cut(s) 74, 295, 614
BseXI GCAGC 3 cut(s) 38, 661, 677
BseYI CCCAGC 1 cut(s) 591
BshFI GGCC 7 cut(s) 72, 141, 258, 305, 338, 385, 562
BshNI GGYRCC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 3 cut(s) 255, 266, 302
BslI CCNNNNNNNGG 4 cut(s) 265, 301, 307, 659
BsnI GGCC 7 cut(s) 72, 141, 258, 305, 338, 385, 562
BsoBI CYCGRG 1 cut(s) 265
Bsp143I GATC 1 cut(s) 279
BspACI CCGC 3 cut(s) 252, 311, 318
BspANI GGCC 7 cut(s) 72, 141, 258, 305, 338, 385, 562
BspLI GGNNCC 2 cut(s) 331, 480
BspMI ACCTGC 1 cut(s) 314
BspOI GCTAGC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 3 cut(s) 74, 295, 614
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 4 cut(s) 132, 265, 306, 333
BssMI GATC 1 cut(s) 279
BssNI GRCGYC 1 cut(s) 330
BssT1I CCWWGG 2 cut(s) 132, 333
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 3 cut(s) 169, 238, 526
BstACI GRCGYC 1 cut(s) 330
BstAPI GCANNNNNTGC 1 cut(s) 352
BstC8I GCNNGC 4 cut(s) 200, 217, 256, 316
BstDEI CTNAG 2 cut(s) 473, 483
BstF5I GGATG 3 cut(s) 92, 339, 406
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 1 cut(s) 282
BstMBI GATC 1 cut(s) 279
BstMWI GCNNNNNNNGC 7 cut(s) 195, 311, 320, 329, 352, 671, 674
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 4 cut(s) 264, 265, 300, 306
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 38, 661, 677
BstV2I GAAGAC 1 cut(s) 676
BstXI CCANNNNNNTGG 1 cut(s) 172
BsuRI GGCC 7 cut(s) 72, 141, 258, 305, 338, 385, 562
BtsCI GGATG 3 cut(s) 92, 339, 406
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 4 cut(s) 200, 217, 256, 316
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 8 cut(s) 71, 129, 175, 304, 383, 479, 560, 650
Cfr9I CCCGGG 1 cut(s) 265
Csp6I GTAC 2 cut(s) 247, 553
CviAII CATG 4 cut(s) 55, 79, 161, 190
CviQI GTAC 2 cut(s) 247, 553
DdeI CTNAG 2 cut(s) 473, 483
DinI GGCGCC 1 cut(s) 331
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
EciI GGCGGA 1 cut(s) 333
Eco130I CCWWGG 2 cut(s) 132, 333
Eco47I GGWCC 4 cut(s) 129, 175, 479, 650
Eco57I CTGAAG 1 cut(s) 444
Eco88I CYCGRG 1 cut(s) 265
EcoO109I RGGNCCY 4 cut(s) 129, 383, 479, 560
EcoRII CCWGG 1 cut(s) 306
EcoT14I CCWWGG 2 cut(s) 132, 333
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 2 cut(s) 132, 333
FaeI CATG 4 cut(s) 58, 82, 164, 193
FatI CATG 4 cut(s) 54, 78, 160, 189
Fnu4HI GCNGC 5 cut(s) 27, 252, 312, 666, 675
FokI GGATG 3 cut(s) 79, 326, 413
Fsp4HI GCNGC 5 cut(s) 27, 252, 312, 666, 675
FspBI CTAG 3 cut(s) 126, 216, 678
GlaI GCGC 1 cut(s) 331
GluI GCNGC 5 cut(s) 27, 252, 312, 666, 675
GsaI CCCAGC 1 cut(s) 595
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 7 cut(s) 72, 141, 258, 305, 338, 385, 562
HapII CCGG 3 cut(s) 255, 266, 302
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 4 cut(s) 58, 82, 164, 193
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 1 cut(s) 487
HpaII CCGG 3 cut(s) 255, 266, 302
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 435, 555
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 2 cut(s) 178, 688
Hpy8I GTNNAC 2 cut(s) 435, 555
HpyAV CCTTC 3 cut(s) 396, 419, 573
HpyCH4III ACNGT 3 cut(s) 169, 238, 526
HpyCH4V TGCA 3 cut(s) 41, 346, 499
HpyF10VI GCNNNNNNNGC 7 cut(s) 195, 311, 320, 329, 352, 671, 674
HpyF3I CTNAG 2 cut(s) 473, 483
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 4 cut(s) 58, 82, 164, 193
HspAI GCGC 1 cut(s) 330
KasI GGCGCC 1 cut(s) 329
KroI GCCGGC 1 cut(s) 254
KroNI GCCGGC 1 cut(s) 256
Kzo9I GATC 1 cut(s) 279
LmnI GCTCC 3 cut(s) 320, 625, 662
Lsp1109I GCAGC 3 cut(s) 38, 661, 677
LweI GCATC 2 cut(s) 28, 342
MaeI CTAG 3 cut(s) 126, 216, 678
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MboII GAAGA 2 cut(s) 16, 676
MluCI AATT 2 cut(s) 42, 657
Mly113I GGCGCC 1 cut(s) 330
MnlI CCTC 3 cut(s) 238, 269, 711
MroNI GCCGGC 1 cut(s) 254
MseI TTAA 2 cut(s) 513, 656
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 3 cut(s) 255, 266, 302
MspR9I CCNGG 4 cut(s) 266, 267, 302, 308
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 7 cut(s) 195, 311, 320, 329, 352, 671, 674
NaeI GCCGGC 1 cut(s) 256
NarI GGCGCC 1 cut(s) 330
NciI CCSGG 3 cut(s) 266, 267, 302
NdeII GATC 1 cut(s) 279
NgoMIV GCCGGC 1 cut(s) 254
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 4 cut(s) 58, 82, 164, 193
NlaIV GGNNCC 2 cut(s) 331, 480
NspI RCATGY 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 314
PdiI GCCGGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 487
PkrI GCNGC 5 cut(s) 28, 253, 313, 667, 676
PluTI GGCGCC 1 cut(s) 333
PpuMI RGGWCCY 2 cut(s) 129, 479
Psp5II RGGWCCY 2 cut(s) 129, 479
Psp6I CCWGG 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 591
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 331, 480
PspPI GGNCC 8 cut(s) 71, 129, 175, 304, 383, 479, 560, 650
PspPPI RGGWCCY 2 cut(s) 129, 479
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 248, 554
RsaNI GTAC 2 cut(s) 247, 553
SaqAI TTAA 2 cut(s) 513, 656
SatI GCNGC 5 cut(s) 27, 252, 312, 666, 675
Sau3AI GATC 1 cut(s) 279
Sau96I GGNCC 8 cut(s) 71, 129, 175, 304, 383, 479, 560, 650
ScrFI CCNGG 4 cut(s) 266, 267, 302, 308
SfaNI GCATC 2 cut(s) 28, 342
SfcI CTRYAG 1 cut(s) 30
SfoI GGCGCC 1 cut(s) 331
SinI GGWCC 4 cut(s) 129, 175, 479, 650
SmaI CCCGGG 1 cut(s) 267
Sse9I AATT 2 cut(s) 42, 657
SsiI CCGC 3 cut(s) 252, 311, 318
SspDI GGCGCC 1 cut(s) 329
SspMI CTAG 3 cut(s) 126, 216, 678
StyD4I CCNGG 4 cut(s) 264, 265, 300, 306
StyI CCWWGG 2 cut(s) 132, 333
TaaI ACNGT 3 cut(s) 169, 238, 526
TasI AATT 2 cut(s) 42, 657
TauI GCSGC 2 cut(s) 254, 314
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 2 cut(s) 513, 656
Tru9I TTAA 2 cut(s) 513, 656
TscAI CASTG 1 cut(s) 241
TseI GCWGC 3 cut(s) 26, 665, 674
TspDTI ATGAA 6 cut(s) 17, 55, 96, 136, 381, 584
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 4 cut(s) 129, 175, 479, 650
XceI RCATGY 1 cut(s) 164
XmaI CCCGGG 1 cut(s) 265
XspI CTAG 3 cut(s) 126, 216, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.