pycom09g03280

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002246
Physical Location & Seq
Reverse (-)
2451891 .. 2452930
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g03280.1

Sequence Viewer

Length: 954 bp
ATGGGTCAATTATTTATATGCTGTCTAAACCACCTTACCCTTAATTTAAAAACGTTAACGTTTTGCTCGTGTATTCCAATAATTTTGGTGGGTAGGCAAGTGGTTGGTGTAAGATGCCCACAAGTTTCACATGTTATTCTCCCGCATCGGTGGAACACACCACCAATATATACAGGTAAAAAACAAAACAAATTCAACAGAGAAAAATCAGTGGAGCAAAAGAAAGATAAGATGGGGGTGATCAAGGCAGCTGCAGGAGATGCAATTTTGACCTCTATGTGGGTGTTCAGTGCACCCAGCATGGGGGTTTTCACATCCATCATTGCCTCGTTTCTTGGCATCCAAGCTAGGTCTTTGGTAGGTCTTTTCATCACCACAATAATTTCGACACTTGTGGTTTTAATATTCAGCTTGATTGGCAAGTTGTTGGGTGGTGCCAGTTTCAACCCCTCCACCACCGCCAGTTTCTACGCCGCCGGCCTCAATCCCGGAACTTCCCTCCTCTCCATGGCGGTTCGGTTCCCGGCTCAAGCCGCCGGCGGAGTAGGCGGTGCCAAGGCAATTTTGCAAGTGATGCCAAAAAAGTACAAGCACATGCTTAAAGGGCCTTCTTTGAAAGTGGATTTGTATACTGGTGTTATAGCTGAGGGGGTGATGACTTGTGTTCTTTGCTTTGCCTTGCTTGTGATTATACTGAGAGGGCCTAGAAATCCAATTGTGAACATATGGATGCTTTCGGTCACGACGCTCGGATTGGTGGTTGCTGGAAATGGGTACACGGGGCCTTCCATGAACCCCGCCAATGCTTTTGGGTGGGCTTATGTGAACAATTGGCACAACAGTTGGGAGCTTTTCTTGGTTTATTGGATTGGCCCCTTTGTTGGAGCAATTGTAGCTGCTTTGGCTTTTAGGCTTTGGTTTCCCCCACCAGTACCGAAGGAAAAGAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

318

Amino Acids

34.24

Weight (kDa)

9.97

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 81 - 303 8.9e-17 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 434, 551
AccI GTMKAC 1 cut(s) 629
AciI CCGC 8 cut(s) 143, 459, 474, 512, 534, 540, 549, 798
AclI AACGTT 2 cut(s) 53, 59
AcsI RAATTY 1 cut(s) 191
AfaI GTAC 3 cut(s) 587, 776, 933
AfiI CCNNNNNNNGG 5 cut(s) 279, 302, 303, 508, 881
AflIII ACRYGT 1 cut(s) 130
AgsI TTSAA 3 cut(s) 196, 445, 616
AluBI AGCT 7 cut(s) 251, 347, 411, 644, 850, 896, 950
AluI AGCT 7 cut(s) 251, 347, 411, 644, 850, 896, 950
Alw21I GWGCWC 1 cut(s) 295
Alw44I GTGCAC 1 cut(s) 291
AoxI GGCC 5 cut(s) 478, 605, 701, 782, 871
ApaLI GTGCAC 1 cut(s) 291
ApeKI GCWGC 3 cut(s) 248, 251, 896
ApoI RAATTY 1 cut(s) 191
AspS9I GGNCC 4 cut(s) 605, 701, 782, 872
AsuC2I CCSGG 2 cut(s) 489, 524
AsuHPI GGTGA 3 cut(s) 250, 364, 664
BaeGI GKGCMC 1 cut(s) 295
BanI GGYRCC 2 cut(s) 434, 551
BauI CACGAG 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 645
BbvI GCAGC 3 cut(s) 238, 260, 883
BccI CCATC 2 cut(s) 226, 326
BclI TGATCA 1 cut(s) 240
BcnI CCSGG 2 cut(s) 489, 524
BfaI CTAG 2 cut(s) 348, 705
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 5 cut(s) 249, 252, 474, 534, 897
BlsI GCNGC 5 cut(s) 250, 253, 475, 535, 898
Bme1390I CCNGG 2 cut(s) 489, 524
BmgT120I GGNCC 4 cut(s) 605, 701, 782, 872
BmiI GGNNCC 5 cut(s) 436, 521, 553, 783, 874
BmrFI CCNGG 2 cut(s) 489, 524
BmsI GCATC 6 cut(s) 104, 154, 250, 348, 564, 720
Bpu10I CCTNAGC 1 cut(s) 645
BpuEI CTTGAG 1 cut(s) 513
BpuMI CCSGG 2 cut(s) 489, 524
BsaJI CCNNGG 2 cut(s) 507, 555
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 5 cut(s) 279, 302, 303, 508, 881
Bse118I RCCGGY 2 cut(s) 476, 536
Bse1I ACTGG 4 cut(s) 438, 462, 637, 929
Bse3DI GCAATG 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 507, 555
BseGI GGATG 3 cut(s) 314, 339, 735
BseLI CCNNNNNNNGG 5 cut(s) 279, 302, 303, 508, 881
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 636, 686
BseNI ACTGG 4 cut(s) 438, 462, 637, 929
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 3 cut(s) 238, 260, 883
BseYI CCCAGC 1 cut(s) 296
BshFI GGCC 5 cut(s) 480, 607, 703, 784, 873
BshNI GGYRCC 2 cut(s) 434, 551
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 4 cut(s) 477, 489, 524, 537
BslI CCNNNNNNNGG 5 cut(s) 279, 302, 303, 508, 881
BsnI GGCC 5 cut(s) 480, 607, 703, 784, 873
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 8 cut(s) 143, 459, 474, 512, 534, 540, 549, 798
BspANI GGCC 5 cut(s) 480, 607, 703, 784, 873
BspCNI CTCAG 2 cut(s) 637, 687
BspLI GGNNCC 5 cut(s) 436, 521, 553, 783, 874
BspMAI CTGCAG 1 cut(s) 256
BspT107I GGYRCC 2 cut(s) 434, 551
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 2 cut(s) 476, 536
BsrI ACTGG 4 cut(s) 438, 462, 637, 929
BssAI RCCGGY 2 cut(s) 476, 536
BssECI CCNNGG 2 cut(s) 507, 555
BssMI GATC 1 cut(s) 240
BssNAI GTATAC 1 cut(s) 630
BssSI CACGAG 1 cut(s) 67
BssT1I CCWWGG 2 cut(s) 507, 555
Bst1107I GTATAC 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 67
Bst4CI ACNGT 1 cut(s) 842
BstAPI GCANNNNNTGC 2 cut(s) 260, 574
BstC8I GCNNGC 2 cut(s) 478, 538
BstDEI CTNAG 2 cut(s) 645, 695
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 3 cut(s) 314, 339, 735
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMWI GCNNNNNNNGC 8 cut(s) 260, 417, 533, 546, 574, 604, 893, 902
BstNSI RCATGY 2 cut(s) 134, 598
BstSCI CCNGG 2 cut(s) 487, 522
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 3 cut(s) 238, 260, 883
BstZ17I GTATAC 1 cut(s) 630
BsuRI GGCC 5 cut(s) 480, 607, 703, 784, 873
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 314, 339, 735
BtsIMutI CAGTG 2 cut(s) 216, 295
Cac8I GCNNGC 2 cut(s) 478, 538
Cfr10I RCCGGY 2 cut(s) 476, 536
Cfr13I GGNCC 4 cut(s) 605, 701, 782, 872
CseI GACGC 1 cut(s) 754
Csp6I GTAC 3 cut(s) 586, 775, 932
CviAII CATG 5 cut(s) 131, 301, 508, 595, 790
CviQI GTAC 3 cut(s) 586, 775, 932
DdeI CTNAG 2 cut(s) 645, 695
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DraI TTTAAA 1 cut(s) 48
EciI GGCGGA 1 cut(s) 555
Eco130I CCWWGG 2 cut(s) 507, 555
EcoO109I RGGNCCY 3 cut(s) 605, 701, 782
EcoT14I CCWWGG 2 cut(s) 507, 555
ErhI CCWWGG 2 cut(s) 507, 555
FaeI CATG 5 cut(s) 134, 304, 511, 598, 793
FatI CATG 5 cut(s) 130, 300, 507, 594, 789
FauI CCCGC 2 cut(s) 150, 805
FauNDI CATATG 1 cut(s) 725
FbaI TGATCA 1 cut(s) 240
FblI GTMKAC 1 cut(s) 629
Fnu4HI GCNGC 5 cut(s) 249, 252, 474, 534, 897
FokI GGATG 3 cut(s) 301, 326, 742
Fsp4HI GCNGC 5 cut(s) 249, 252, 474, 534, 897
FspBI CTAG 2 cut(s) 348, 705
GluI GCNGC 5 cut(s) 249, 252, 474, 534, 897
GsaI CCCAGC 1 cut(s) 300
HaeIII GGCC 5 cut(s) 480, 607, 703, 784, 873
HapII CCGG 4 cut(s) 477, 489, 524, 537
HgaI GACGC 1 cut(s) 754
Hin1II CATG 5 cut(s) 134, 304, 511, 598, 793
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 948
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 4 cut(s) 477, 489, 524, 537
HphI GGTGA 3 cut(s) 250, 364, 664
Hpy166II GTNNAC 6 cut(s) 57, 293, 630, 721, 777, 826
Hpy188I TCNGA 1 cut(s) 752
Hpy188III TCNNGA 1 cut(s) 742
Hpy8I GTNNAC 6 cut(s) 57, 293, 630, 721, 777, 826
Hpy99I CGWCG 1 cut(s) 748
HpyAV CCTTC 3 cut(s) 618, 795, 931
HpyCH4III ACNGT 1 cut(s) 842
HpyCH4IV ACGT 2 cut(s) 53, 59
HpyCH4V TGCA 4 cut(s) 254, 263, 293, 568
HpyF10VI GCNNNNNNNGC 8 cut(s) 260, 417, 533, 546, 574, 604, 893, 902
HpyF3I CTNAG 2 cut(s) 645, 695
HpySE526I ACGT 2 cut(s) 53, 59
Hsp92II CATG 5 cut(s) 134, 304, 511, 598, 793
KroI GCCGGC 2 cut(s) 476, 536
KroNI GCCGGC 2 cut(s) 478, 538
Ksp22I TGATCA 1 cut(s) 240
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 214, 847, 884
Lsp1109I GCAGC 3 cut(s) 238, 260, 883
LweI GCATC 6 cut(s) 104, 154, 250, 348, 564, 720
MaeI CTAG 2 cut(s) 348, 705
MaeII ACGT 2 cut(s) 53, 59
MaeIII GTNAC 1 cut(s) 739
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MfeI CAATTG 3 cut(s) 714, 829, 888
MhlI GDGCHC 1 cut(s) 295
MmeI TCCRAC 1 cut(s) 862
MnlI CCTC 8 cut(s) 283, 337, 460, 491, 509, 512, 640, 692
MreI CGCCGGCG 1 cut(s) 536
MroNI GCCGGC 2 cut(s) 476, 536
MseI TTAA 5 cut(s) 42, 47, 56, 401, 600
MslI CAYNNNNRTG 1 cut(s) 728
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 4 cut(s) 477, 489, 524, 537
MspR9I CCNGG 2 cut(s) 489, 524
MunI CAATTG 3 cut(s) 714, 829, 888
MwoI GCNNNNNNNGC 8 cut(s) 260, 417, 533, 546, 574, 604, 893, 902
NaeI GCCGGC 2 cut(s) 478, 538
NciI CCSGG 2 cut(s) 489, 524
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 725
NdeII GATC 1 cut(s) 240
NgoMIV GCCGGC 2 cut(s) 476, 536
NlaIII CATG 5 cut(s) 134, 304, 511, 598, 793
NlaIV GGNNCC 5 cut(s) 436, 521, 553, 783, 874
NmuCI GTSAC 1 cut(s) 739
NspI RCATGY 2 cut(s) 134, 598
PciI ACATGT 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PdiI GCCGGC 2 cut(s) 478, 538
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 5 cut(s) 250, 253, 475, 535, 898
PscI ACATGT 1 cut(s) 130
Psp1406I AACGTT 2 cut(s) 53, 59
PspFI CCCAGC 1 cut(s) 296
PspN4I GGNNCC 5 cut(s) 436, 521, 553, 783, 874
PspPI GGNCC 4 cut(s) 605, 701, 782, 872
PstI CTGCAG 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 3 cut(s) 587, 776, 933
RsaNI GTAC 3 cut(s) 586, 775, 932
RseI CAYNNNNRTG 1 cut(s) 728
SaqAI TTAA 5 cut(s) 42, 47, 56, 401, 600
SatI GCNGC 5 cut(s) 249, 252, 474, 534, 897
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 4 cut(s) 605, 701, 782, 872
ScrFI CCNGG 2 cut(s) 489, 524
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 6 cut(s) 104, 154, 250, 348, 564, 720
SfcI CTRYAG 1 cut(s) 252
SgrAI CRCCGGYG 1 cut(s) 536
SmiMI CAYNNNNRTG 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
SsiI CCGC 8 cut(s) 143, 459, 474, 512, 534, 540, 549, 798
SspI AATATT 1 cut(s) 405
SspMI CTAG 2 cut(s) 348, 705
StyD4I CCNGG 2 cut(s) 487, 522
StyI CCWWGG 2 cut(s) 507, 555
TaaI ACNGT 1 cut(s) 842
TaiI ACGT 2 cut(s) 56, 62
TaqI TCGA 1 cut(s) 386
TaqII GACCGA 1 cut(s) 727
TatI WGTACW 1 cut(s) 585
TauI GCSGC 2 cut(s) 476, 536
Tru1I TTAA 5 cut(s) 42, 47, 56, 401, 600
Tru9I TTAA 5 cut(s) 42, 47, 56, 401, 600
TscAI CASTG 2 cut(s) 216, 295
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 3 cut(s) 248, 251, 896
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 2 cut(s) 358, 806
TspRI CASTG 2 cut(s) 216, 295
VneI GTGCAC 1 cut(s) 291
XapI RAATTY 1 cut(s) 191
XceI RCATGY 2 cut(s) 134, 598
XmiI GTMKAC 1 cut(s) 629
XspI CTAG 2 cut(s) 348, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.