FvH4_6g43030

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002246
Physical Location & Seq
Forward (+)
33357695 .. 33358855
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43030.t1

Sequence Viewer

Length: 726 bp
ATGGGTAATCTGATCAAAGCAGCTGTAGGAGATGCAATTCTGACTTCCCTATTTGTTTTCGTTGCCCCAATCATGCGGGTGTTCACAGCCATCTTAGCTTCATTTCTTGGATTCCAAGCAAGGTCCTTGATAGGCCGTTTCATCACCACCATACATGCCACAGTTCTAGTCCTGATATTCAGCATGATTGGCAAGCTCTTGGGTGGTGCCAGCTTCAATCCTTCCACCACTGTCAGTTTCTACGCTGCCGGCCTCAACCCGGGAACTTCCCTGATCTCTATGGCAGTTCAGTTCCCGGCTCAGGCCGTCGGCGGAGCAGGTGGCGCCAAGGCCATCCTGCAACTGATGCCTGAAAAGTATGAGCAATTTGTCAAAGGGCCTTCTCTGAAAGTGGACTTGAAAAGTGGTGCTATTGCTGAAGGGTTGTTCACTTTTTCTCTTTGTTTTTCTCTGCTTTGGGTTATGCTCAAGGGTCCTAAGAATCTTATTCTGCAAATATGGTTGGTTAAAGTGGCTACTGTGGGATTGGCTATAGTGGGGAGTGGATACACAGGGCCTTCAATGAACCCTGCTAATGCTTTTGGCTGGGCTTATGTTAATAACTGGCACAATACTTGGGAGCTCTTCTTTGTATATTGGATTGGTCCTTTAGTTGGAGCAGTTTTGGCTGCTAGAGTCTTCAAGATTTTGTTTCCTCCACCAGAAACAAAGGAGAAGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

242

Amino Acids

25.79

Weight (kDa)

9.87

Isoelectric Point (pI)

24.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 5 - 226 4.7e-17 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 206, 323
AciI CCGC 2 cut(s) 76, 312
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 324
AfiI CCNNNNNNNGG 3 cut(s) 259, 301, 653
AgsI TTSAA 4 cut(s) 217, 400, 561, 682
AluBI AGCT 5 cut(s) 23, 98, 196, 213, 622
AluI AGCT 5 cut(s) 23, 98, 196, 213, 622
Alw21I GWGCWC 1 cut(s) 624
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 6 cut(s) 133, 250, 303, 330, 377, 554
ApeKI GCWGC 3 cut(s) 20, 245, 668
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 5 cut(s) 123, 377, 473, 554, 644
AsuC2I CCSGG 3 cut(s) 260, 261, 296
AsuHPI GGTGA 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 3 cut(s) 123, 473, 644
BanI GGYRCC 2 cut(s) 206, 323
BanII GRGCYC 1 cut(s) 624
BbsI GAAGAC 1 cut(s) 670
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 3 cut(s) 32, 232, 655
BccI CCATC 2 cut(s) 98, 341
BceAI ACGGC 2 cut(s) 120, 290
BciVI GTATCC 1 cut(s) 539
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 3 cut(s) 260, 261, 296
BfaI CTAG 2 cut(s) 167, 672
BfmI CTRYAG 2 cut(s) 24, 531
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 308
BfuI GTATCC 1 cut(s) 539
BisI GCNGC 3 cut(s) 21, 246, 669
BlsI GCNGC 3 cut(s) 22, 247, 670
Bme1390I CCNGG 3 cut(s) 260, 261, 296
Bme18I GGWCC 3 cut(s) 123, 473, 644
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 5 cut(s) 123, 377, 473, 554, 644
BmiI GGNNCC 3 cut(s) 208, 325, 474
BmrFI CCNGG 3 cut(s) 260, 261, 296
BmsI GCATC 2 cut(s) 22, 336
BpiI GAAGAC 1 cut(s) 670
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 3 cut(s) 260, 261, 296
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 259, 327
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 301, 653
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 608
BseDI CCNNGG 2 cut(s) 259, 327
BseGI GGATG 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 259, 301, 653
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 608
BseXI GCAGC 3 cut(s) 32, 232, 655
BseYI CCCAGC 1 cut(s) 585
BshFI GGCC 6 cut(s) 135, 252, 305, 332, 379, 556
BshNI GGYRCC 2 cut(s) 206, 323
BsiHKAI GWGCWC 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 3 cut(s) 249, 260, 296
BslI CCNNNNNNNGG 3 cut(s) 259, 301, 653
BsnI GGCC 6 cut(s) 135, 252, 305, 332, 379, 556
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 624
Bsp143I GATC 2 cut(s) 12, 273
BspACI CCGC 2 cut(s) 76, 312
BspANI GGCC 6 cut(s) 135, 252, 305, 332, 379, 556
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 208, 325, 474
BspMI ACCTGC 1 cut(s) 308
BspQI GCTCTTC 1 cut(s) 629
BspT107I GGYRCC 2 cut(s) 206, 323
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 608
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 259, 327
BssMI GATC 2 cut(s) 12, 273
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 327
Bst4CI ACNGT 3 cut(s) 163, 232, 520
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 194, 211, 250
BstDEI CTNAG 3 cut(s) 94, 300, 477
BstF5I GGATG 1 cut(s) 333
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 2 cut(s) 15, 276
BstMBI GATC 2 cut(s) 12, 273
BstMWI GCNNNNNNNGC 5 cut(s) 95, 189, 323, 346, 665
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 258, 259, 294
BstSFI CTRYAG 2 cut(s) 24, 531
BstV1I GCAGC 3 cut(s) 32, 232, 655
BstV2I GAAGAC 1 cut(s) 670
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 6 cut(s) 135, 252, 305, 332, 379, 556
BtsCI GGATG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 228
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 3 cut(s) 194, 211, 250
CfoI GCGC 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 5 cut(s) 123, 377, 473, 554, 644
Cfr9I CCCGGG 1 cut(s) 259
CviAII CATG 3 cut(s) 73, 155, 184
DdeI CTNAG 3 cut(s) 94, 300, 477
DinI GGCGCC 1 cut(s) 325
DpnI GATC 2 cut(s) 14, 275
DpnII GATC 2 cut(s) 12, 273
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 624
Eco47I GGWCC 3 cut(s) 123, 473, 644
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 622
EcoO109I RGGNCCY 4 cut(s) 123, 377, 473, 554
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 624
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 3 cut(s) 76, 158, 187
FatI CATG 3 cut(s) 72, 154, 183
FauI CCCGC 1 cut(s) 69
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 21, 246, 669
FokI GGATG 1 cut(s) 320
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 3 cut(s) 21, 246, 669
FspBI CTAG 2 cut(s) 167, 672
GlaI GCGC 1 cut(s) 325
GluI GCNGC 3 cut(s) 21, 246, 669
GsaI CCCAGC 1 cut(s) 589
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 6 cut(s) 135, 252, 305, 332, 379, 556
HapII CCGG 3 cut(s) 249, 260, 296
HhaI GCGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 3 cut(s) 76, 158, 187
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 3 cut(s) 111, 481, 675
HpaII CCGG 3 cut(s) 249, 260, 296
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 3 cut(s) 84, 394, 429
Hpy188I TCNGA 3 cut(s) 12, 42, 387
Hpy188III TCNNGA 2 cut(s) 172, 682
Hpy8I GTNNAC 3 cut(s) 84, 394, 429
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 231, 390, 413, 567
HpyCH4III ACNGT 3 cut(s) 163, 232, 520
HpyCH4V TGCA 3 cut(s) 35, 340, 493
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 189, 323, 346, 665
HpyF3I CTNAG 3 cut(s) 94, 300, 477
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 3 cut(s) 76, 158, 187
HspAI GCGC 1 cut(s) 324
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 2 cut(s) 12, 273
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 3 cut(s) 314, 619, 656
Lsp1109I GCAGC 3 cut(s) 32, 232, 655
LweI GCATC 2 cut(s) 22, 336
MaeI CTAG 2 cut(s) 167, 672
MalI GATC 2 cut(s) 14, 275
MboI GATC 2 cut(s) 12, 273
MboII GAAGA 2 cut(s) 616, 670
MhlI GDGCHC 1 cut(s) 624
MluCI AATT 2 cut(s) 36, 365
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 684
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 2 cut(s) 263, 705
MroNI GCCGGC 1 cut(s) 248
MseI TTAA 2 cut(s) 507, 597
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 249, 260, 296
MspR9I CCNGG 3 cut(s) 260, 261, 296
MwoI GCNNNNNNNGC 5 cut(s) 95, 189, 323, 346, 665
NaeI GCCGGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 324
NciI CCSGG 3 cut(s) 260, 261, 296
NdeII GATC 2 cut(s) 12, 273
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 3 cut(s) 76, 158, 187
NlaIV GGNNCC 3 cut(s) 208, 325, 474
NspI RCATGY 1 cut(s) 158
PaqCI CACCTGC 1 cut(s) 308
PciSI GCTCTTC 1 cut(s) 629
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 111, 481
PkrI GCNGC 3 cut(s) 22, 247, 670
PleI GAGTC 1 cut(s) 683
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 683
PpuMI RGGWCCY 2 cut(s) 123, 473
Psp124BI GAGCTC 1 cut(s) 624
Psp5II RGGWCCY 2 cut(s) 123, 473
PspFI CCCAGC 1 cut(s) 585
PspN4I GGNNCC 3 cut(s) 208, 325, 474
PspPI GGNCC 5 cut(s) 123, 377, 473, 554, 644
PspPPI RGGWCCY 2 cut(s) 123, 473
PvuII CAGCTG 1 cut(s) 23
RseI CAYNNNNRTG 1 cut(s) 77
SacI GAGCTC 1 cut(s) 624
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 2 cut(s) 507, 597
SatI GCNGC 3 cut(s) 21, 246, 669
Sau3AI GATC 2 cut(s) 12, 273
Sau96I GGNCC 5 cut(s) 123, 377, 473, 554, 644
SchI GAGTC 1 cut(s) 684
ScrFI CCNGG 3 cut(s) 260, 261, 296
SduI GDGCHC 1 cut(s) 624
SetI ASST 7 cut(s) 25, 100, 125, 198, 215, 322, 624
SfaNI GCATC 2 cut(s) 22, 336
SfcI CTRYAG 2 cut(s) 24, 531
SfoI GGCGCC 1 cut(s) 325
SinI GGWCC 3 cut(s) 123, 473, 644
SmaI CCCGGG 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 2 cut(s) 36, 365
SsiI CCGC 2 cut(s) 76, 312
SspDI GGCGCC 1 cut(s) 323
SspMI CTAG 2 cut(s) 167, 672
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 3 cut(s) 258, 259, 294
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 3 cut(s) 163, 232, 520
TasI AATT 2 cut(s) 36, 365
TfiI GAWTC 2 cut(s) 111, 481
Tru1I TTAA 2 cut(s) 507, 597
Tru9I TTAA 2 cut(s) 507, 597
TscAI CASTG 1 cut(s) 235
TseI GCWGC 3 cut(s) 20, 245, 668
TspDTI ATGAA 3 cut(s) 90, 130, 578
TspMI CCCGGG 1 cut(s) 259
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 3 cut(s) 123, 473, 644
XceI RCATGY 1 cut(s) 158
XmaI CCCGGG 1 cut(s) 259
XspI CTAG 2 cut(s) 167, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.