MD17G1098100.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002246
Physical Location & Seq
Reverse (-)
8343658 .. 8344380
723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1098100.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGGGGTGATCAAGGCAGCTGCAGGAGATGCAATTTTGACCTCCATGTGGGTGTTCTGTGCACCCAGCATGGGAGTTTTCACATCCATCATAGCCTCGTTTCTTGGCATCCAAGCTAGGTCTTTGTCAGGTCTTTTCATCACCACAATAATTTCCACAGTTCTGATTTTAACAATCAGCTTGATAGGCAAGCTCTTGGGTGGTGCCAGCTTCAACCCCTCCACCACCGCCAGTTTCTACACTGCTGGTCTCAACCCGGGAACTTCTCTCCTCTCCATGGCAGTTCGGTTCCCGGCTCAGGCGGCCGGCGGAGTAGGTGGCGCCAAGGCAATTTTACAAGTGATGCCAAAGAAGTACAAGCACATGCTTAGAGGGCCTTCTTTGAAAGTGGATTTGTATACTGGTATTATAGCTGAGGGGGTGATGACTTGTCTTCTTTGCTTTGCTTTGCTTGTGATTATGCTGAGAGGGCCTAGAAATCCAATTGTGAAGATATGGATGCTTTCGATCGTGACGATTGGATTGGTGGTCGCCGGAAATGGGTACACAGGACCTTCCATGAACCCTGCCAATGCTTTTGGGTGGGCGTATGTAAATAATTGGCACAACAGTTGGGAGCTTTTCTTGGTTTATTGGATTGGCCCCTTTGTTGGAGCAACTGTAGCTGCTTTGGTTTTTAGGGTTTTGTTTCCCCCACCAGTACCAAAGGAAAAGAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

241

Amino Acids

25.43

Weight (kDa)

9.97

Isoelectric Point (pI)

24.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 15 - 226 3.2e-17 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 203, 320
AccI GTMKAC 1 cut(s) 398
AciI CCGC 3 cut(s) 228, 302, 309
AcoI YGGCCR 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 3 cut(s) 356, 545, 702
AfiI CCNNNNNNNGG 6 cut(s) 48, 71, 277, 298, 540, 650
AgsI TTSAA 2 cut(s) 214, 385
AluBI AGCT 9 cut(s) 20, 116, 180, 193, 210, 413, 619, 665, 719
AluI AGCT 9 cut(s) 20, 116, 180, 193, 210, 413, 619, 665, 719
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 254
Alw44I GTGCAC 1 cut(s) 60
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 4 cut(s) 303, 374, 470, 640
ApaLI GTGCAC 1 cut(s) 60
ApeKI GCWGC 3 cut(s) 17, 20, 665
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 4 cut(s) 374, 470, 551, 641
AsuC2I CCSGG 3 cut(s) 257, 258, 293
AsuHPI GGTGA 3 cut(s) 19, 133, 433
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 64
BanI GGYRCC 2 cut(s) 203, 320
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 64
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 3 cut(s) 7, 29, 652
BccI CCATC 1 cut(s) 95
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 3 cut(s) 257, 258, 293
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 2 cut(s) 117, 474
BfmI CTRYAG 2 cut(s) 21, 660
BfoI RGCGCY 1 cut(s) 324
BisI GCNGC 4 cut(s) 18, 21, 303, 666
BlsI GCNGC 4 cut(s) 19, 22, 304, 667
Bme1390I CCNGG 3 cut(s) 257, 258, 293
Bme18I GGWCC 1 cut(s) 551
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 374, 470, 551, 641
BmiI GGNNCC 4 cut(s) 205, 290, 322, 643
BmrFI CCNGG 3 cut(s) 257, 258, 293
BmsI GCATC 4 cut(s) 19, 117, 333, 489
BpiI GAAGAC 1 cut(s) 425
Bpu10I CCTNAGC 2 cut(s) 297, 414
BpuMI CCSGG 3 cut(s) 257, 258, 293
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 256, 276, 324
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 71, 277, 298, 540, 650
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 231, 406, 698
BseDI CCNNGG 3 cut(s) 256, 276, 324
BseGI GGATG 3 cut(s) 83, 108, 504
BseLI CCNNNNNNNGG 6 cut(s) 48, 71, 277, 298, 540, 650
BseMII CTCAG 3 cut(s) 311, 405, 455
BseNI ACTGG 3 cut(s) 231, 406, 698
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 64
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 3 cut(s) 7, 29, 652
BseYI CCCAGC 1 cut(s) 65
Bsh1285I CGRYCG 2 cut(s) 306, 510
BshFI GGCC 4 cut(s) 305, 376, 472, 642
BshNI GGYRCC 2 cut(s) 203, 320
BsiEI CGRYCG 2 cut(s) 306, 510
BsiHKAI GWGCWC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 4 cut(s) 257, 293, 306, 534
BslI CCNNNNNNNGG 6 cut(s) 48, 71, 277, 298, 540, 650
BsmAI GTCTC 1 cut(s) 254
BsnI GGCC 4 cut(s) 305, 376, 472, 642
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 2 cut(s) 9, 507
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 3 cut(s) 228, 302, 309
BspANI GGCC 4 cut(s) 305, 376, 472, 642
BspCNI CTCAG 3 cut(s) 310, 406, 456
BspLI GGNNCC 4 cut(s) 205, 290, 322, 643
BspMAI CTGCAG 1 cut(s) 25
BspT107I GGYRCC 2 cut(s) 203, 320
BspTNI GGTCTC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 3 cut(s) 231, 406, 698
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 256, 276, 324
BssMI GATC 2 cut(s) 9, 507
BssNAI GTATAC 1 cut(s) 399
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 276, 324
Bst1107I GTATAC 1 cut(s) 399
Bst4CI ACNGT 3 cut(s) 160, 611, 661
BstACI GRCGYC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 191, 208, 307
BstDEI CTNAG 4 cut(s) 297, 368, 414, 464
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 83, 108, 504
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 2 cut(s) 12, 510
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 2 cut(s) 9, 507
BstMCI CGRYCG 2 cut(s) 306, 510
BstMWI GCNNNNNNNGC 6 cut(s) 29, 186, 302, 373, 469, 662
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 3 cut(s) 255, 256, 291
BstSFI CTRYAG 2 cut(s) 21, 660
BstSLI GKGCMC 1 cut(s) 64
BstV1I GCAGC 3 cut(s) 7, 29, 652
BstV2I GAAGAC 1 cut(s) 425
BstZ17I GTATAC 1 cut(s) 399
BstZI CGGCCG 1 cut(s) 303
BsuRI GGCC 4 cut(s) 305, 376, 472, 642
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 3 cut(s) 83, 108, 504
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 3 cut(s) 191, 208, 307
CfoI GCGC 1 cut(s) 323
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 4 cut(s) 374, 470, 551, 641
Cfr9I CCCGGG 1 cut(s) 256
Csp6I GTAC 3 cut(s) 355, 544, 701
CviAII CATG 5 cut(s) 46, 70, 277, 364, 559
CviQI GTAC 3 cut(s) 355, 544, 701
DdeI CTNAG 4 cut(s) 297, 368, 414, 464
DinI GGCGCC 1 cut(s) 322
DpnI GATC 2 cut(s) 11, 509
DpnII GATC 2 cut(s) 9, 507
EaeI YGGCCR 1 cut(s) 303
EagI CGGCCG 1 cut(s) 303
EciI GGCGGA 1 cut(s) 324
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 2 cut(s) 276, 324
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 551
Eco52I CGGCCG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 256
EcoO109I RGGNCCY 3 cut(s) 374, 470, 551
EcoT14I CCWWGG 2 cut(s) 276, 324
EgeI GGCGCC 1 cut(s) 322
EheI GGCGCC 1 cut(s) 322
ErhI CCWWGG 2 cut(s) 276, 324
FaeI CATG 5 cut(s) 49, 73, 280, 367, 562
FatI CATG 5 cut(s) 45, 69, 276, 363, 558
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 4 cut(s) 18, 21, 303, 666
FokI GGATG 3 cut(s) 70, 95, 511
Fsp4HI GCNGC 4 cut(s) 18, 21, 303, 666
FspBI CTAG 2 cut(s) 117, 474
GlaI GCGC 1 cut(s) 322
GluI GCNGC 4 cut(s) 18, 21, 303, 666
GsaI CCCAGC 1 cut(s) 69
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 4 cut(s) 305, 376, 472, 642
HapII CCGG 4 cut(s) 257, 293, 306, 534
HhaI GCGC 1 cut(s) 323
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 5 cut(s) 49, 73, 280, 367, 562
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 717
HpaII CCGG 4 cut(s) 257, 293, 306, 534
HphI GGTGA 3 cut(s) 19, 133, 433
Hpy166II GTNNAC 3 cut(s) 62, 399, 546
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 1 cut(s) 511
Hpy8I GTNNAC 3 cut(s) 62, 399, 546
HpyAV CCTTC 2 cut(s) 387, 564
HpyCH4III ACNGT 3 cut(s) 160, 611, 661
HpyCH4V TGCA 3 cut(s) 23, 32, 62
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 186, 302, 373, 469, 662
HpyF3I CTNAG 4 cut(s) 297, 368, 414, 464
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 5 cut(s) 49, 73, 280, 367, 562
HspAI GCGC 1 cut(s) 321
KasI GGCGCC 1 cut(s) 320
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 2 cut(s) 9, 507
LmnI GCTCC 2 cut(s) 616, 653
Lsp1109I GCAGC 3 cut(s) 7, 29, 652
LweI GCATC 4 cut(s) 19, 117, 333, 489
MaeI CTAG 2 cut(s) 117, 474
MaeIII GTNAC 1 cut(s) 511
MalI GATC 2 cut(s) 11, 509
MboI GATC 2 cut(s) 9, 507
MboII GAAGA 2 cut(s) 425, 502
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 5 cut(s) 33, 150, 330, 483, 598
Mly113I GGCGCC 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 631
MnlI CCTC 7 cut(s) 52, 106, 229, 281, 365, 409, 461
MroNI GCCGGC 1 cut(s) 305
MseI TTAA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 4 cut(s) 257, 293, 306, 534
MspR9I CCNGG 3 cut(s) 257, 258, 293
MunI CAATTG 1 cut(s) 483
MwoI GCNNNNNNNGC 6 cut(s) 29, 186, 302, 373, 469, 662
NaeI GCCGGC 1 cut(s) 307
NarI GGCGCC 1 cut(s) 321
NciI CCSGG 3 cut(s) 257, 258, 293
NcoI CCATGG 1 cut(s) 276
NdeII GATC 2 cut(s) 9, 507
NgoMIV GCCGGC 1 cut(s) 305
NlaIII CATG 5 cut(s) 49, 73, 280, 367, 562
NlaIV GGNNCC 4 cut(s) 205, 290, 322, 643
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PdiI GCCGGC 1 cut(s) 307
PkrI GCNGC 4 cut(s) 19, 22, 304, 667
Ple19I CGATCG 1 cut(s) 510
PluTI GGCGCC 1 cut(s) 324
PpuMI RGGWCCY 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 551
PspFI CCCAGC 1 cut(s) 65
PspN4I GGNNCC 4 cut(s) 205, 290, 322, 643
PspPI GGNCC 4 cut(s) 374, 470, 551, 641
PspPPI RGGWCCY 1 cut(s) 551
PstI CTGCAG 1 cut(s) 25
PvuI CGATCG 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 356, 545, 702
RsaNI GTAC 3 cut(s) 355, 544, 701
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 4 cut(s) 18, 21, 303, 666
Sau3AI GATC 2 cut(s) 9, 507
Sau96I GGNCC 4 cut(s) 374, 470, 551, 641
ScrFI CCNGG 3 cut(s) 257, 258, 293
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 4 cut(s) 19, 117, 333, 489
SfcI CTRYAG 2 cut(s) 21, 660
SfoI GGCGCC 1 cut(s) 322
SinI GGWCC 1 cut(s) 551
SmaI CCCGGG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 5 cut(s) 33, 150, 330, 483, 598
SsiI CCGC 3 cut(s) 228, 302, 309
SspDI GGCGCC 1 cut(s) 320
SspMI CTAG 2 cut(s) 117, 474
StyD4I CCNGG 3 cut(s) 255, 256, 291
StyI CCWWGG 2 cut(s) 276, 324
TaaI ACNGT 3 cut(s) 160, 611, 661
TaqI TCGA 1 cut(s) 506
TasI AATT 5 cut(s) 33, 150, 330, 483, 598
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 3 cut(s) 17, 20, 665
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 2 cut(s) 127, 575
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 1 cut(s) 247
VneI GTGCAC 1 cut(s) 60
VpaK11BI GGWCC 1 cut(s) 551
XceI RCATGY 1 cut(s) 367
XmaI CCCGGG 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 398
XspI CTAG 2 cut(s) 117, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.