Rh2BG552800

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002246
Physical Location & Seq
Forward (+)
76120966 .. 76123298
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG552800.1

Sequence Viewer

Length: 726 bp
ATGGGTAGTCTGATCAAAGCAGCTCTGGGAGATGCAGTTTTGACCTCCATGTGGGTTTTCAGCGCCCCATCCATGGGGGTTTTCACAACCATCATAGCATCGTTTCTTGGAGTCCAAGCAAGGTCCTTGATAGGCCTTTTCATCACCACCGTACTCGCCACAATTTTGGTATTGACTTTCAGCTTGATTGGCAAGCTCTTGGGTGGTGCCAGCTTCAATCCATCCACCACAGCAAGCTTCTACGCCGCCGGCCTCAGTCCGGCAACCTCCCTGATCTCGATGGCTGTCAGGTTCCCGGCTCAAGCGGCCGGCGGGGTAGGCGGCGCCAAGGCCATCCTGCAGGTGATGCCTGACAAGTTCAAGCATTTTGTCAAAGGGCCTTCTCTGAAAGTGGATGTGAAAAGTGGTGCCGTTGCTGAAGGGTTGTTCACTTTTTCTCTTTGCTTTGCTTTGCTTTGGATTATACTTAGGGGTCCTAAGAATCCAATATTGCAAATCTGGTTGCTTTCTATGGCTACTGTGGGATTGGTTGTTGCGGGGAGTGGGTTCACAGGGCCTTCTATGAACCCTGCTAATGCTTATGGCTGGGCTTATGTCAATAACTGGCACAACACTTGGGAGCTTTTCTTTGTTTATTGGATTGGTCCTTTAATTGGAGCAATTTTGGCTGCTAGGGTCTTCAAGGTTTTGTTTCCTCCACCAGAACCAAAGGAGAAGAAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

241

Amino Acids

25.49

Weight (kDa)

9.96

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 6 - 226 5.5e-15 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
Acc36I ACCTGC 1 cut(s) 331
AccB1I GGYRCC 3 cut(s) 206, 323, 407
AciI CCGC 5 cut(s) 246, 305, 312, 321, 536
AcoI YGGCCR 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 5 cut(s) 51, 73, 74, 259, 653
AgsI TTSAA 3 cut(s) 217, 361, 682
AluBI AGCT 6 cut(s) 23, 183, 196, 213, 237, 622
AluI AGCT 6 cut(s) 23, 183, 196, 213, 237, 622
AoxI GGCC 6 cut(s) 133, 250, 306, 330, 377, 554
ApeKI GCWGC 2 cut(s) 20, 668
AspLEI GCGC 2 cut(s) 65, 326
AspS9I GGNCC 5 cut(s) 123, 377, 473, 554, 644
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 136, 355
AvaII GGWCC 3 cut(s) 123, 473, 644
BanI GGYRCC 3 cut(s) 206, 323, 407
BbsI GAAGAC 1 cut(s) 670
BbvI GCAGC 2 cut(s) 32, 655
BccI CCATC 5 cut(s) 76, 98, 229, 274, 341
BceAI ACGGC 1 cut(s) 395
BclI TGATCA 1 cut(s) 12
BcnI CCSGG 1 cut(s) 296
BfaI CTAG 1 cut(s) 672
BfmI CTRYAG 1 cut(s) 338
BfoI RGCGCY 2 cut(s) 66, 327
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 5 cut(s) 21, 246, 306, 322, 669
BlsI GCNGC 5 cut(s) 22, 247, 307, 323, 670
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 3 cut(s) 123, 473, 644
BmgT120I GGNCC 5 cut(s) 123, 377, 473, 554, 644
BmiI GGNNCC 5 cut(s) 208, 293, 325, 409, 474
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 3 cut(s) 22, 107, 336
BpiI GAAGAC 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 72, 327
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 73, 74, 259, 653
Bse118I RCCGGY 2 cut(s) 248, 308
Bse1I ACTGG 1 cut(s) 608
BseDI CCNNGG 2 cut(s) 72, 327
BseGI GGATG 4 cut(s) 68, 221, 333, 400
BseLI CCNNNNNNNGG 5 cut(s) 51, 73, 74, 259, 653
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 608
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 2 cut(s) 32, 655
BseYI CCCAGC 1 cut(s) 585
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
BshNI GGYRCC 3 cut(s) 206, 323, 407
BsiEI CGRYCG 1 cut(s) 309
BsiSI CCGG 4 cut(s) 249, 260, 296, 309
BslI CCNNNNNNNGG 5 cut(s) 51, 73, 74, 259, 653
BsnI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
Bsp143I GATC 2 cut(s) 12, 273
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 5 cut(s) 246, 305, 312, 321, 536
BspANI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
BspCNI CTCAG 1 cut(s) 267
BspLI GGNNCC 5 cut(s) 208, 293, 325, 409, 474
BspMAI CTGCAG 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 331
BspT107I GGYRCC 3 cut(s) 206, 323, 407
BsrFI RCCGGY 2 cut(s) 248, 308
BsrI ACTGG 1 cut(s) 608
BssAI RCCGGY 2 cut(s) 248, 308
BssECI CCNNGG 2 cut(s) 72, 327
BssMI GATC 2 cut(s) 12, 273
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 72, 327
Bst4CI ACNGT 2 cut(s) 151, 520
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 5 cut(s) 194, 211, 235, 250, 310
BstDEI CTNAG 3 cut(s) 254, 467, 477
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 4 cut(s) 68, 221, 333, 400
BstH2I RGCGCY 2 cut(s) 66, 327
BstHHI GCGC 2 cut(s) 65, 326
BstKTI GATC 2 cut(s) 15, 276
BstMBI GATC 2 cut(s) 12, 273
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 5 cut(s) 189, 305, 318, 346, 665
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 338
BstV1I GCAGC 2 cut(s) 32, 655
BstV2I GAAGAC 1 cut(s) 670
BstXI CCANNNNNNTGG 1 cut(s) 166
BstZI CGGCCG 1 cut(s) 306
BsuRI GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 4 cut(s) 68, 221, 333, 400
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 5 cut(s) 194, 211, 235, 250, 310
CfoI GCGC 2 cut(s) 65, 326
Cfr10I RCCGGY 2 cut(s) 248, 308
Cfr13I GGNCC 5 cut(s) 123, 377, 473, 554, 644
Csp6I GTAC 1 cut(s) 152
CviAII CATG 2 cut(s) 49, 73
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 3 cut(s) 254, 467, 477
DinI GGCGCC 1 cut(s) 325
DpnI GATC 2 cut(s) 14, 275
DpnII GATC 2 cut(s) 12, 273
EaeI YGGCCR 1 cut(s) 306
EagI CGGCCG 1 cut(s) 306
EclXI CGGCCG 1 cut(s) 306
Eco130I CCWWGG 2 cut(s) 72, 327
Eco147I AGGCCT 1 cut(s) 135
Eco47I GGWCC 3 cut(s) 123, 473, 644
Eco52I CGGCCG 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 438
EcoO109I RGGNCCY 4 cut(s) 123, 377, 473, 554
EcoT14I CCWWGG 2 cut(s) 72, 327
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 72, 327
FaeI CATG 2 cut(s) 52, 76
FaiI YATR 8 cut(s) 50, 74, 95, 464, 512, 563, 582, 594
FatI CATG 2 cut(s) 48, 72
FauI CCCGC 2 cut(s) 305, 529
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 5 cut(s) 21, 246, 306, 322, 669
FokI GGATG 4 cut(s) 55, 208, 320, 407
Fsp4HI GCNGC 5 cut(s) 21, 246, 306, 322, 669
FspBI CTAG 1 cut(s) 672
GlaI GCGC 2 cut(s) 64, 325
GluI GCNGC 5 cut(s) 21, 246, 306, 322, 669
GsaI CCCAGC 1 cut(s) 589
HaeII RGCGCY 2 cut(s) 66, 327
HaeIII GGCC 6 cut(s) 135, 252, 308, 332, 379, 556
HapII CCGG 4 cut(s) 249, 260, 296, 309
HhaI GCGC 2 cut(s) 65, 326
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 2 cut(s) 52, 76
Hin6I GCGC 2 cut(s) 63, 324
HinP1I GCGC 2 cut(s) 63, 324
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 2 cut(s) 111, 481
HpaII CCGG 4 cut(s) 249, 260, 296, 309
HphI GGTGA 2 cut(s) 136, 355
Hpy166II GTNNAC 2 cut(s) 429, 549
Hpy188I TCNGA 2 cut(s) 12, 387
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 2 cut(s) 429, 549
HpyAV CCTTC 3 cut(s) 390, 413, 567
HpyCH4III ACNGT 2 cut(s) 151, 520
HpyCH4V TGCA 3 cut(s) 35, 340, 493
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 305, 318, 346, 665
HpyF3I CTNAG 3 cut(s) 254, 467, 477
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 2 cut(s) 52, 76
HspAI GCGC 2 cut(s) 63, 324
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 2 cut(s) 248, 308
KroNI GCCGGC 2 cut(s) 250, 310
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 2 cut(s) 12, 273
LmnI GCTCC 2 cut(s) 619, 656
Lsp1109I GCAGC 2 cut(s) 32, 655
LweI GCATC 3 cut(s) 22, 107, 336
MaeI CTAG 1 cut(s) 672
MalI GATC 2 cut(s) 14, 275
MboI GATC 2 cut(s) 12, 273
MboII GAAGA 1 cut(s) 670
MluCI AATT 3 cut(s) 162, 651, 660
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 4 cut(s) 55, 263, 277, 705
MroNI GCCGGC 2 cut(s) 248, 308
MseI TTAA 1 cut(s) 650
MspI CCGG 4 cut(s) 249, 260, 296, 309
MspR9I CCNGG 1 cut(s) 296
MwoI GCNNNNNNNGC 5 cut(s) 189, 305, 318, 346, 665
NaeI GCCGGC 2 cut(s) 250, 310
NarI GGCGCC 1 cut(s) 324
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 72
NdeII GATC 2 cut(s) 12, 273
NgoMIV GCCGGC 2 cut(s) 248, 308
NlaIII CATG 2 cut(s) 52, 76
NlaIV GGNNCC 5 cut(s) 208, 293, 325, 409, 474
PaqCI CACCTGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 135
PdiI GCCGGC 2 cut(s) 250, 310
PfeI GAWTC 1 cut(s) 481
PkrI GCNGC 5 cut(s) 22, 247, 307, 323, 670
PleI GAGTC 1 cut(s) 119
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 2 cut(s) 123, 473
Psp5II RGGWCCY 2 cut(s) 123, 473
PspFI CCCAGC 1 cut(s) 585
PspN4I GGNNCC 5 cut(s) 208, 293, 325, 409, 474
PspPI GGNCC 5 cut(s) 123, 377, 473, 554, 644
PspPPI RGGWCCY 2 cut(s) 123, 473
PstI CTGCAG 1 cut(s) 342
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 650
SatI GCNGC 5 cut(s) 21, 246, 306, 322, 669
Sau3AI GATC 2 cut(s) 12, 273
Sau96I GGNCC 5 cut(s) 123, 377, 473, 554, 644
SbfI CCTGCAGG 1 cut(s) 342
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 1 cut(s) 296
SdaI CCTGCAGG 1 cut(s) 342
SfaNI GCATC 3 cut(s) 22, 107, 336
SfcI CTRYAG 1 cut(s) 338
SfoI GGCGCC 1 cut(s) 325
SinI GGWCC 3 cut(s) 123, 473, 644
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse8387I CCTGCAGG 1 cut(s) 342
Sse9I AATT 3 cut(s) 162, 651, 660
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 5 cut(s) 246, 305, 312, 321, 536
SspDI GGCGCC 1 cut(s) 323
SspI AATATT 1 cut(s) 489
SspMI CTAG 1 cut(s) 672
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 2 cut(s) 72, 327
TaaI ACNGT 2 cut(s) 151, 520
TaqI TCGA 1 cut(s) 278
TasI AATT 3 cut(s) 162, 651, 660
TauI GCSGC 3 cut(s) 248, 308, 324
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 650
Tru9I TTAA 1 cut(s) 650
TseI GCWGC 2 cut(s) 20, 668
TspDTI ATGAA 2 cut(s) 130, 578
VpaK11BI GGWCC 3 cut(s) 123, 473, 644
XspI CTAG 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.