MD09G1110400.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002246
Physical Location & Seq
Reverse (-)
8195457 .. 8196179
723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1110400.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGGGGTGATCAAGGCAGCTGCAGGAGATGCAATTTTGACATCTATGTGGGTGTTCAGTGCACCCAGCATGGGGGTTTTCACATCCATCATTGCTTCGTTTCTTGGTATCCAAGCTAGGTCTTTGGTAGGTCTTTTCATCACCACAATAATTTCGACACTTGTGGTTTTAATATTCAGCTTGATTGGCAAGTTGTTGGGCGGTGCCAGTTTCAACCCCTCCACCACCGTCAGTTTCTACACCGCCGGCCTCAATCCCGGAACTTCCCTCCTCTCCATGGCTGTTCGGTTCCCGGCTCAGGCGGCGGGCGGAGTAGGCGGTGCCAAGGCAATTTTGCAGGTGATGCCAAAAAAGTACAAGCACATGCTTAAAGGGCCTTCTTTGAAAGTGGATTTGTATACTGGTGTTATAGCTGAGGGGGTGATGACTTGTGTTCTTTGCTTTGCCTTGCTTGTGATTATAATGAGAGGGCCTAGAAATCCAATTGTGAACATATGGATGATTTCGGTCACGACGCTCGGATTGGTGGTTGCCGGAAATGGGTACACGGGGCCTTCCATGAACCCGGCCAATGCTTTTGGGTGGGCTTATGTGAACAATTGGCACAACAGTTGGGAGCTTTTCTTGGTTTATTGGATTGGCCCCTTTGTTGGAGCAACTGTAGCAGCTTCGGCTTTTAGGGTTTTGTTTCCCCCACCGGTACCAAAGGAAAAGAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

241

Amino Acids

25.35

Weight (kDa)

9.96

Isoelectric Point (pI)

22.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 43 - 224 2.6e-16 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 461
AarI CACCTGC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 328
Acc65I GGTACC 1 cut(s) 700
AccB1I GGYRCC 3 cut(s) 203, 320, 700
AccI GTMKAC 1 cut(s) 398
AciI CCGC 6 cut(s) 201, 243, 302, 305, 309, 318
AcoI YGGCCR 1 cut(s) 567
AfaI GTAC 3 cut(s) 356, 545, 702
AfiI CCNNNNNNNGG 6 cut(s) 71, 72, 277, 298, 540, 650
AgeI ACCGGT 1 cut(s) 697
AgsI TTSAA 2 cut(s) 214, 385
AluBI AGCT 7 cut(s) 20, 116, 180, 413, 619, 668, 719
AluI AGCT 7 cut(s) 20, 116, 180, 413, 619, 668, 719
Alw21I GWGCWC 1 cut(s) 64
Alw44I GTGCAC 1 cut(s) 60
AoxI GGCC 6 cut(s) 247, 374, 470, 551, 567, 640
ApaLI GTGCAC 1 cut(s) 60
ApeKI GCWGC 3 cut(s) 17, 20, 665
AsiGI ACCGGT 1 cut(s) 697
Asp718I GGTACC 1 cut(s) 700
AspS9I GGNCC 4 cut(s) 374, 470, 551, 641
AsuC2I CCSGG 3 cut(s) 258, 293, 566
AsuHPI GGTGA 4 cut(s) 19, 133, 352, 433
BaeGI GKGCMC 1 cut(s) 64
BanI GGYRCC 3 cut(s) 203, 320, 700
Bbv12I GWGCWC 1 cut(s) 64
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 3 cut(s) 7, 29, 677
BccI CCATC 1 cut(s) 95
BciVI GTATCC 1 cut(s) 119
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 3 cut(s) 258, 293, 566
BfaI CTAG 2 cut(s) 117, 474
BfmI CTRYAG 2 cut(s) 21, 660
BfuAI ACCTGC 1 cut(s) 328
BfuI GTATCC 1 cut(s) 119
BisI GCNGC 4 cut(s) 18, 21, 303, 666
BlsI GCNGC 4 cut(s) 19, 22, 304, 667
Bme1390I CCNGG 3 cut(s) 258, 293, 566
BmgT120I GGNCC 4 cut(s) 374, 470, 551, 641
BmiI GGNNCC 6 cut(s) 205, 290, 322, 552, 643, 702
BmrFI CCNGG 3 cut(s) 258, 293, 566
BmsI GCATC 2 cut(s) 19, 333
Bpu10I CCTNAGC 2 cut(s) 297, 414
BpuMI CCSGG 3 cut(s) 258, 293, 566
BsaJI CCNNGG 2 cut(s) 276, 324
BsaWI WCCGGW 1 cut(s) 697
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 72, 277, 298, 540, 650
Bse118I RCCGGY 2 cut(s) 245, 697
Bse1I ACTGG 2 cut(s) 207, 406
Bse3DI GCAATG 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 276, 324
BseGI GGATG 2 cut(s) 83, 504
BseLI CCNNNNNNNGG 6 cut(s) 71, 72, 277, 298, 540, 650
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 2 cut(s) 311, 405
BseNI ACTGG 2 cut(s) 207, 406
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 3 cut(s) 7, 29, 677
BseYI CCCAGC 1 cut(s) 65
BshFI GGCC 6 cut(s) 249, 376, 472, 553, 569, 642
BshNI GGYRCC 3 cut(s) 203, 320, 700
BshTI ACCGGT 1 cut(s) 697
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 6 cut(s) 246, 258, 293, 534, 566, 698
BslI CCNNNNNNNGG 6 cut(s) 71, 72, 277, 298, 540, 650
BsnI GGCC 6 cut(s) 249, 376, 472, 553, 569, 642
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 6 cut(s) 201, 243, 302, 305, 309, 318
BspANI GGCC 6 cut(s) 249, 376, 472, 553, 569, 642
BspCNI CTCAG 2 cut(s) 310, 406
BspLI GGNNCC 6 cut(s) 205, 290, 322, 552, 643, 702
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 328
BspT107I GGYRCC 3 cut(s) 203, 320, 700
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 245, 697
BsrI ACTGG 2 cut(s) 207, 406
BssAI RCCGGY 2 cut(s) 245, 697
BssECI CCNNGG 2 cut(s) 276, 324
BssMI GATC 1 cut(s) 9
BssNAI GTATAC 1 cut(s) 399
BssT1I CCWWGG 2 cut(s) 276, 324
Bst1107I GTATAC 1 cut(s) 399
Bst4CI ACNGT 3 cut(s) 229, 611, 661
BstAPI GCANNNNNTGC 2 cut(s) 29, 343
BstC8I GCNNGC 2 cut(s) 247, 307
BstDEI CTNAG 2 cut(s) 297, 414
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 2 cut(s) 83, 504
BstKTI GATC 1 cut(s) 12
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 8 cut(s) 29, 186, 302, 315, 343, 373, 662, 671
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 3 cut(s) 256, 291, 564
BstSFI CTRYAG 2 cut(s) 21, 660
BstSLI GKGCMC 1 cut(s) 64
BstV1I GCAGC 3 cut(s) 7, 29, 677
BstZ17I GTATAC 1 cut(s) 399
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 6 cut(s) 249, 376, 472, 553, 569, 642
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 83, 504
BtsIMutI CAGTG 1 cut(s) 64
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 247, 307
Cfr10I RCCGGY 2 cut(s) 245, 697
Cfr13I GGNCC 4 cut(s) 374, 470, 551, 641
CseI GACGC 1 cut(s) 523
Csp6I GTAC 3 cut(s) 355, 544, 701
CspAI ACCGGT 1 cut(s) 697
CviAII CATG 4 cut(s) 70, 277, 364, 559
CviQI GTAC 3 cut(s) 355, 544, 701
DdeI CTNAG 2 cut(s) 297, 414
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 567
EciI GGCGGA 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 276, 324
EcoO109I RGGNCCY 3 cut(s) 374, 470, 551
EcoT14I CCWWGG 2 cut(s) 276, 324
ErhI CCWWGG 2 cut(s) 276, 324
FaeI CATG 4 cut(s) 73, 280, 367, 562
FatI CATG 4 cut(s) 69, 276, 363, 558
FauI CCCGC 1 cut(s) 298
FauNDI CATATG 1 cut(s) 494
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 4 cut(s) 18, 21, 303, 666
FokI GGATG 2 cut(s) 70, 511
Fsp4HI GCNGC 4 cut(s) 18, 21, 303, 666
FspBI CTAG 2 cut(s) 117, 474
GluI GCNGC 4 cut(s) 18, 21, 303, 666
GsaI CCCAGC 1 cut(s) 69
HaeIII GGCC 6 cut(s) 249, 376, 472, 553, 569, 642
HapII CCGG 6 cut(s) 246, 258, 293, 534, 566, 698
HgaI GACGC 1 cut(s) 523
Hin1II CATG 4 cut(s) 73, 280, 367, 562
HindIII AAGCTT 1 cut(s) 717
HpaII CCGG 6 cut(s) 246, 258, 293, 534, 566, 698
HphI GGTGA 4 cut(s) 19, 133, 352, 433
Hpy166II GTNNAC 5 cut(s) 62, 399, 490, 546, 595
Hpy188I TCNGA 1 cut(s) 521
Hpy188III TCNNGA 1 cut(s) 511
Hpy8I GTNNAC 5 cut(s) 62, 399, 490, 546, 595
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 2 cut(s) 387, 564
HpyCH4III ACNGT 3 cut(s) 229, 611, 661
HpyCH4V TGCA 4 cut(s) 23, 32, 62, 337
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 186, 302, 315, 343, 373, 662, 671
HpyF3I CTNAG 2 cut(s) 297, 414
Hsp92II CATG 4 cut(s) 73, 280, 367, 562
KpnI GGTACC 1 cut(s) 704
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 2 cut(s) 616, 653
Lsp1109I GCAGC 3 cut(s) 7, 29, 677
LweI GCATC 2 cut(s) 19, 333
MaeI CTAG 2 cut(s) 117, 474
MaeIII GTNAC 1 cut(s) 508
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MfeI CAATTG 2 cut(s) 483, 598
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 5 cut(s) 33, 150, 330, 483, 598
MmeI TCCRAC 1 cut(s) 631
MnlI CCTC 6 cut(s) 229, 260, 278, 281, 409, 461
MroNI GCCGGC 1 cut(s) 245
MseI TTAA 2 cut(s) 170, 369
MslI CAYNNNNRTG 2 cut(s) 46, 497
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 6 cut(s) 246, 258, 293, 534, 566, 698
MspR9I CCNGG 3 cut(s) 258, 293, 566
MunI CAATTG 2 cut(s) 483, 598
MwoI GCNNNNNNNGC 8 cut(s) 29, 186, 302, 315, 343, 373, 662, 671
NaeI GCCGGC 1 cut(s) 247
NciI CCSGG 3 cut(s) 258, 293, 566
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 494
NdeII GATC 1 cut(s) 9
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 4 cut(s) 73, 280, 367, 562
NlaIV GGNNCC 6 cut(s) 205, 290, 322, 552, 643, 702
NmuCI GTSAC 1 cut(s) 508
NspI RCATGY 1 cut(s) 367
PaqCI CACCTGC 1 cut(s) 328
PdiI GCCGGC 1 cut(s) 247
PfoI TCCNGGA 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 697
PkrI GCNGC 4 cut(s) 19, 22, 304, 667
PsiI TTATAA 1 cut(s) 461
PspFI CCCAGC 1 cut(s) 65
PspN4I GGNNCC 6 cut(s) 205, 290, 322, 552, 643, 702
PspPI GGNCC 4 cut(s) 374, 470, 551, 641
PstI CTGCAG 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 356, 545, 702
RsaNI GTAC 3 cut(s) 355, 544, 701
RseI CAYNNNNRTG 2 cut(s) 46, 497
SaqAI TTAA 2 cut(s) 170, 369
SatI GCNGC 4 cut(s) 18, 21, 303, 666
Sau3AI GATC 1 cut(s) 9
Sau96I GGNCC 4 cut(s) 374, 470, 551, 641
ScrFI CCNGG 3 cut(s) 258, 293, 566
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 2 cut(s) 19, 333
SfcI CTRYAG 2 cut(s) 21, 660
SmiMI CAYNNNNRTG 2 cut(s) 46, 497
Sse9I AATT 5 cut(s) 33, 150, 330, 483, 598
SsiI CCGC 6 cut(s) 201, 243, 302, 305, 309, 318
SspI AATATT 1 cut(s) 174
SspMI CTAG 2 cut(s) 117, 474
StyD4I CCNGG 3 cut(s) 256, 291, 564
StyI CCWWGG 2 cut(s) 276, 324
TaaI ACNGT 3 cut(s) 229, 611, 661
TaqI TCGA 1 cut(s) 155
TaqII GACCGA 1 cut(s) 496
TasI AATT 5 cut(s) 33, 150, 330, 483, 598
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 305
Tru1I TTAA 2 cut(s) 170, 369
Tru9I TTAA 2 cut(s) 170, 369
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 3 cut(s) 17, 20, 665
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 2 cut(s) 127, 575
TspRI CASTG 1 cut(s) 64
VneI GTGCAC 1 cut(s) 60
XceI RCATGY 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 398
XspI CTAG 2 cut(s) 117, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.