RchiOBHm_Chr2g0159031

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002246
Physical Location & Seq
Forward (+)
75130360 .. 75131272
913 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52769

Sequence Viewer

Length: 447 bp
ATGGCAGTCCAGTTCCCGGCTCAGGCGGCCGGCGGAGCAGGTGGCGCCAAGGCCATCCTGCAAGTGATGCCTAAAAAGTATGAACATTTTGTCAAAGGGCCTTCTCTGAAAGTGGATGTGAAAAGTGGTGCTATTGCTGAAGGGTTGTTCACTTTTTCTCTTTGCTTTTCTCTGCTTTGGGTTATGCTTAGGGGTCCTAAGAATCTTTTTCTGCAAATATGGTTGGTTAAAGTGGCCACTGTGGGATTGGCTATTGTGGGAAGTGGGTACACAGGGCCTTCTATGAACCCTGCTAATGCTTTTGGCTGGGCTTATGTTGATAACTGGCACAATACTTGGGAGCTGTTCTATGTTTATTGGGTTGGTCCTTTAATTGGAGCAGCTTTGGCTGCTAGGGTCTTCAAGATTTTGTTTCCTCCACCAGAAACAAAGGAGAAGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

148

Amino Acids

16.1

Weight (kDa)

9.64

Isoelectric Point (pI)

22.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 7 - 133 2.4e-14 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 29
Acc36I ACCTGC 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 44
AciI CCGC 2 cut(s) 26, 33
AcoI YGGCCR 2 cut(s) 27, 234
AcuI CTGAAG 1 cut(s) 159
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 16, 22, 374
AgsI TTSAA 1 cut(s) 403
AluBI AGCT 2 cut(s) 343, 383
AluI AGCT 2 cut(s) 343, 383
AoxI GGCC 5 cut(s) 27, 51, 98, 234, 275
ApeKI GCWGC 2 cut(s) 380, 389
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 4 cut(s) 98, 194, 275, 365
AsuC2I CCSGG 1 cut(s) 17
AvaII GGWCC 2 cut(s) 194, 365
BalI TGGCCA 1 cut(s) 236
BanI GGYRCC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 391
BbvI GCAGC 2 cut(s) 376, 392
BccI CCATC 1 cut(s) 62
BcnI CCSGG 1 cut(s) 17
BfaI CTAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 29
BisI GCNGC 3 cut(s) 27, 381, 390
BlsI GCNGC 3 cut(s) 28, 382, 391
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 2 cut(s) 194, 365
BmgT120I GGNCC 4 cut(s) 98, 194, 275, 365
BmiI GGNNCC 2 cut(s) 46, 195
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 391
Bpu10I CCTNAGC 2 cut(s) 21, 188
BpuMI CCSGG 1 cut(s) 17
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 22, 374
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 10, 329
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 54, 121
BseLI CCNNNNNNNGG 3 cut(s) 16, 22, 374
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 2 cut(s) 10, 329
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 376, 392
BseYI CCCAGC 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 5 cut(s) 29, 53, 100, 236, 277
BshNI GGYRCC 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 30
BsiSI CCGG 2 cut(s) 17, 30
BslI CCNNNNNNNGG 3 cut(s) 16, 22, 374
BsnI GGCC 5 cut(s) 29, 53, 100, 236, 277
BspACI CCGC 2 cut(s) 26, 33
BspANI GGCC 5 cut(s) 29, 53, 100, 236, 277
BspCNI CTCAG 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 46, 195
BspMI ACCTGC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 44
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 2 cut(s) 10, 329
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 48
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 1 cut(s) 241
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 3 cut(s) 21, 188, 198
BstF5I GGATG 2 cut(s) 54, 121
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 44, 67, 386, 389
BstSCI CCNGG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 376, 392
BstV2I GAAGAC 1 cut(s) 391
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 5 cut(s) 29, 53, 100, 236, 277
BtsCI GGATG 2 cut(s) 54, 121
BtsIMutI CAGTG 1 cut(s) 237
BveI ACCTGC 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 98, 194, 275, 365
Csp6I GTAC 1 cut(s) 268
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 21, 188, 198
DinI GGCGCC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 27, 234
EagI CGGCCG 1 cut(s) 27
EciI GGCGGA 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 194, 365
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 159
EcoO109I RGGNCCY 3 cut(s) 98, 194, 275
EcoT14I CCWWGG 1 cut(s) 48
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 48
FaiI YATR 6 cut(s) 81, 185, 220, 284, 315, 351
Fnu4HI GCNGC 3 cut(s) 27, 381, 390
FokI GGATG 2 cut(s) 41, 128
Fsp4HI GCNGC 3 cut(s) 27, 381, 390
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 46
GluI GCNGC 3 cut(s) 27, 381, 390
GsaI CCCAGC 1 cut(s) 310
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 5 cut(s) 29, 53, 100, 236, 277
HapII CCGG 2 cut(s) 17, 30
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 45
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HinfI GANTC 1 cut(s) 202
HpaII CCGG 2 cut(s) 17, 30
Hpy166II GTNNAC 2 cut(s) 150, 270
Hpy188I TCNGA 1 cut(s) 108
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 2 cut(s) 150, 270
HpyAV CCTTC 3 cut(s) 111, 134, 288
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 2 cut(s) 61, 214
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 44, 67, 386, 389
HpyF3I CTNAG 3 cut(s) 21, 188, 198
Hsp92I GRCGYC 1 cut(s) 45
HspAI GCGC 1 cut(s) 45
KasI GGCGCC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
LmnI GCTCC 3 cut(s) 35, 340, 377
Lsp1109I GCAGC 2 cut(s) 376, 392
LweI GCATC 1 cut(s) 57
MaeI CTAG 1 cut(s) 393
MboII GAAGA 1 cut(s) 391
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 1 cut(s) 372
MluNI TGGCCA 1 cut(s) 236
Mly113I GGCGCC 1 cut(s) 45
MnlI CCTC 1 cut(s) 426
Mox20I TGGCCA 1 cut(s) 236
MroNI GCCGGC 1 cut(s) 29
MscI TGGCCA 1 cut(s) 236
MseI TTAA 2 cut(s) 228, 371
Msp20I TGGCCA 1 cut(s) 236
MspI CCGG 2 cut(s) 17, 30
MspR9I CCNGG 1 cut(s) 17
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 44, 67, 386, 389
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 17
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 2 cut(s) 46, 195
PaqCI CACCTGC 1 cut(s) 29
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 3 cut(s) 28, 382, 391
PluTI GGCGCC 1 cut(s) 48
PpuMI RGGWCCY 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 46, 195
PspPI GGNCC 4 cut(s) 98, 194, 275, 365
PspPPI RGGWCCY 1 cut(s) 194
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SaqAI TTAA 2 cut(s) 228, 371
SatI GCNGC 3 cut(s) 27, 381, 390
Sau96I GGNCC 4 cut(s) 98, 194, 275, 365
ScrFI CCNGG 1 cut(s) 17
SetI ASST 3 cut(s) 43, 345, 385
SfaNI GCATC 1 cut(s) 57
SfoI GGCGCC 1 cut(s) 46
SinI GGWCC 2 cut(s) 194, 365
Sse9I AATT 1 cut(s) 372
SsiI CCGC 2 cut(s) 26, 33
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 241
TasI AATT 1 cut(s) 372
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 2 cut(s) 228, 371
Tru9I TTAA 2 cut(s) 228, 371
TscAI CASTG 1 cut(s) 244
TseI GCWGC 2 cut(s) 380, 389
TspDTI ATGAA 2 cut(s) 96, 299
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 2 cut(s) 194, 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.