FvH4_7g02290

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002137
Physical Location & Seq
Forward (+)
2633628 .. 2637435
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02290.t1

Sequence Viewer

Length: 1413 bp
ATGGCCCAAGAGGACGGGTCACCTGTTACTTCATCAGCTATGTACTTTCCTGGTTTAGGATCTCCATACCCTTGGCTTTCCCCCGGTATAGGGTCTCCATACCCTTGGCTGAGGGAGCTAAAGTCTGAAGAGAGAGGTTTATGTCTGATCCAACTTCTTCAAGGCTGTGCCCATCATGTTGCTTCTGGTAGTATTGATCAGGCAAATGTTTGGCTCGAGCAGATTTCTCAGCTTGCATCTGCCGGTGGCGATACAATGCAACGAATAGCGGCTTATTTCACTGAGGCACTTGCAGACCGAATGCTGAAAGGTTTGCCTGGTCTATATAAGGCCCTTAACTCAACAAAACTATCATCGATCGCCGAAGAAATTCACATTAAAAGACTTTTCTTTGAGCTCTGTCCCTTCTTGAAGCTTTCATATGTGATCTCAAATCAAGCAATAGTAGAAGCCATGGAGGGGGAGAAGATGGTCCATATTATTGATCTTCATTCATTTGAGCCTGCACAGTGGATAAATCTGATTCAGACATTAAATGCGAGACCTGAAGGTCCACCTCATTTGAGAATTACAGGCATTCATGAACAGAAGGAGGTGTTAGACCAAATGGCGGCCAGGTTGATAGAAGAAGCTGAAAAATATGACATCCCCTTTCAGTTCAATCCTATAGTGAGCAAGTTAGAGAATCTTGATGTTGAAAGTTTACGTGTCAAGACTGGAGAAGCTCTTGCAGTATGCTCCGTACTCCAGCTGCATTCACTCTTAGCAACCGATGATGACATGAACAGAAGAAAATCGCCGTTAGCAACAAAGAATCTGCAAAAAGTCATGCACATCAACCAACTCACTTTAGGTGAATGGCTGGAGAAAGATCCAATCAGTGCATACAGTTTGAGTCCAGATTCTTCTTTATCCCCGCTATCTTTAGGTGCTTCCCCAAAGATGGGGAGCTTTTTAACTGCTCTTTGGGGCCTTTCCCCGAAATTAATGGTGATAACTGAGCAGGAGTCGAACCACAATGGTCATACTCTAATGGATAGGATCATGGAAGCACTGCACTACTATGCAGCACTTTTTGATTGCTTGGAGTCTACAGTATCAAGAGCATCAATGGAGCGGCAAAAGGTTGAGAAGATGCTCTTTGGGGAGGAAATCAAAAACATCATATCATGTGAAGGAACTGACAGAAAGGAGAGGCATGAGAAGCTTGAGAGGTGGATTTTAAGGCTAGAGTTGTCTGGGTTTGGGAGGGTGCAGTTGAGCTACCATGGAAGGATGCAGGCGAAGAAGATGTTGCGAGATTGTGGTTACGACGGCTATGAGATTAAAGAAGACAATGGATGTTTGGTTATTTGCTGGCATGACAGACCACTTTTTTCGGTGTCAGCCTGGAGATTCCGGAGATACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

471

Amino Acids

53.17

Weight (kDa)

5.89

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 49 - 465 1.9e-121 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 549
AccBSI CCGCTC 1 cut(s) 1117
AccI GTMKAC 1 cut(s) 1091
AccIII TCCGGA 1 cut(s) 1398
AciI CCGC 4 cut(s) 269, 611, 917, 1117
AclWI GGATC 4 cut(s) 67, 142, 866, 1049
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 1 cut(s) 369
AcuI CTGAAG 2 cut(s) 147, 567
AfaI GTAC 2 cut(s) 44, 744
AfiI CCNNNNNNNGG 7 cut(s) 56, 89, 90, 459, 610, 943, 944
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 4 cut(s) 161, 412, 661, 698
AjnI CCWGG 4 cut(s) 49, 316, 614, 1388
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 2 cut(s) 99, 535
AlwI GGATC 4 cut(s) 67, 142, 866, 1049
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 1398
AoxI GGCC 4 cut(s) 3, 330, 612, 970
ApeKI GCWGC 2 cut(s) 751, 1067
ApoI RAATTY 1 cut(s) 369
AseI ATTAAT 1 cut(s) 986
Asp700I GAANNNNTTC 1 cut(s) 369
AspS9I GGNCC 5 cut(s) 4, 331, 472, 551, 970
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 12, 866, 1003
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 472, 551
BaeGI GKGCMC 1 cut(s) 172
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 1338
Bbv12I GWGCWC 1 cut(s) 399
BbvCI CCTCAGC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 738, 1079
BccI CCATC 3 cut(s) 180, 463, 937
BceAI ACGGC 2 cut(s) 784, 1330
BciT130I CCWGG 4 cut(s) 51, 318, 616, 1390
BclI TGATCA 1 cut(s) 196
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 2 cut(s) 99, 535
BfaI CTAG 1 cut(s) 1229
BfmI CTRYAG 2 cut(s) 666, 1092
BisI GCNGC 5 cut(s) 270, 612, 752, 1068, 1118
BlsI GCNGC 5 cut(s) 271, 613, 753, 1069, 1119
Bme1390I CCNGG 5 cut(s) 51, 84, 318, 616, 1390
Bme18I GGWCC 2 cut(s) 472, 551
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 5 cut(s) 4, 331, 472, 551, 970
BmiI GGNNCC 1 cut(s) 971
BmrFI CCNGG 5 cut(s) 51, 84, 318, 616, 1390
BmsI GCATC 4 cut(s) 245, 1115, 1125, 1266
BpiI GAAGAC 1 cut(s) 1338
BpmI CTGGAG 4 cut(s) 731, 738, 884, 1411
Bpu10I CCTNAGC 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 1229
BpuMI CCSGG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 707
BsaI GGTCTC 2 cut(s) 99, 535
BsaJI CCNNGG 5 cut(s) 71, 82, 104, 453, 1267
BsaWI WCCGGW 1 cut(s) 1398
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 7 cut(s) 56, 89, 90, 459, 610, 943, 944
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 721
BseAI TCCGGA 1 cut(s) 1398
BseBI CCWGG 4 cut(s) 51, 318, 616, 1390
BseCI ATCGAT 1 cut(s) 356
BseDI CCNNGG 5 cut(s) 71, 82, 104, 453, 1267
BseGI GGATG 3 cut(s) 645, 1281, 1346
BseLI CCNNNNNNNGG 7 cut(s) 56, 89, 90, 459, 610, 943, 944
BseMII CTCAG 4 cut(s) 101, 242, 273, 990
BseNI ACTGG 1 cut(s) 721
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 2 cut(s) 738, 1079
BsgI GTGCAG 3 cut(s) 489, 1040, 1274
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 4 cut(s) 5, 332, 614, 972
BshVI ATCGAT 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 3 cut(s) 84, 243, 1399
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 7 cut(s) 56, 89, 90, 459, 610, 943, 944
BsmAI GTCTC 2 cut(s) 99, 535
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 3 cut(s) 306, 576, 754
BsnI GGCC 4 cut(s) 5, 332, 614, 972
Bso31I GGTCTC 2 cut(s) 99, 535
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 172, 399
Bsp13I TCCGGA 1 cut(s) 1398
Bsp143I GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
Bsp19I CCATGG 2 cut(s) 453, 1267
BspACI CCGC 4 cut(s) 269, 611, 917, 1117
BspANI GGCC 4 cut(s) 5, 332, 614, 972
BspCNI CTCAG 4 cut(s) 102, 241, 274, 991
BspDI ATCGAT 1 cut(s) 356
BspEI TCCGGA 1 cut(s) 1398
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 971
BspPI GGATC 4 cut(s) 67, 142, 866, 1049
BspTNI GGTCTC 2 cut(s) 99, 535
BsrBI CCGCTC 1 cut(s) 1117
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 1 cut(s) 721
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 5 cut(s) 71, 82, 104, 453, 1267
BssMI GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
BssT1I CCWWGG 4 cut(s) 71, 104, 453, 1267
Bst2UI CCWGG 4 cut(s) 51, 318, 616, 1390
Bst4CI ACNGT 3 cut(s) 510, 890, 1096
Bst6I CTCTTC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 4 cut(s) 234, 504, 1281, 1358
BstDEI CTNAG 5 cut(s) 110, 228, 282, 763, 999
BstDSI CCRYGG 2 cut(s) 453, 1267
BstEII GGTNACC 1 cut(s) 18
BstENI CCTNNNNNAGG 1 cut(s) 54
BstF5I GGATG 3 cut(s) 645, 1281, 1346
BstKTI GATC 8 cut(s) 62, 150, 199, 360, 429, 487, 874, 1044
BstMAI GTCTC 2 cut(s) 99, 535
BstMBI GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 2 cut(s) 115, 1204
BstNI CCWGG 4 cut(s) 51, 318, 616, 1390
BstPI GGTNACC 1 cut(s) 18
BstSCI CCNGG 5 cut(s) 49, 82, 316, 614, 1388
BstSFI CTRYAG 2 cut(s) 666, 1092
BstSLI GKGCMC 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 738, 1079
BstV2I GAAGAC 1 cut(s) 1338
BstX2I RGATCY 2 cut(s) 59, 871
BstXI CCANNNNNNTGG 2 cut(s) 72, 105
BstYI RGATCY 2 cut(s) 59, 871
Bsu15I ATCGAT 1 cut(s) 356
BsuRI GGCC 4 cut(s) 5, 332, 614, 972
BsuTUI ATCGAT 1 cut(s) 356
BtgI CCRYGG 2 cut(s) 453, 1267
BtsCI GGATG 3 cut(s) 645, 1281, 1346
BtsI GCAGTG 1 cut(s) 1052
BtsIMutI CAGTG 4 cut(s) 279, 515, 886, 1052
Cac8I GCNNGC 4 cut(s) 234, 504, 1281, 1358
CciI TCATGA 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 5 cut(s) 4, 331, 472, 551, 970
ClaI ATCGAT 1 cut(s) 356
Csp6I GTAC 2 cut(s) 43, 743
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviQI GTAC 2 cut(s) 43, 743
DdeI CTNAG 5 cut(s) 110, 228, 282, 763, 999
DpnI GATC 8 cut(s) 61, 149, 198, 359, 428, 486, 873, 1043
DpnII GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
DrdI GACNNNNNNGTC 1 cut(s) 549
DseDI GACNNNNNNGTC 1 cut(s) 549
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 4 cut(s) 71, 104, 453, 1267
Eco24I GRGCYC 1 cut(s) 399
Eco31I GGTCTC 2 cut(s) 99, 535
Eco47I GGWCC 2 cut(s) 472, 551
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 147, 567
Eco88I CYCGRG 1 cut(s) 215
Eco91I GGTNACC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoO109I RGGNCCY 2 cut(s) 331, 970
EcoO65I GGTNACC 1 cut(s) 18
EcoRII CCWGG 4 cut(s) 49, 316, 614, 1388
EcoT14I CCWWGG 4 cut(s) 71, 104, 453, 1267
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 4 cut(s) 71, 104, 453, 1267
FalI AAGNNNNNCTT 2 cut(s) 1124, 1156
FaqI GGGAC 1 cut(s) 387
FauI CCCGC 1 cut(s) 924
FauNDI CATATG 1 cut(s) 421
FbaI TGATCA 1 cut(s) 196
FblI GTMKAC 1 cut(s) 1091
Fnu4HI GCNGC 5 cut(s) 270, 612, 752, 1068, 1118
FokI GGATG 3 cut(s) 632, 1288, 1353
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 5 cut(s) 270, 612, 752, 1068, 1118
FspBI CTAG 1 cut(s) 1229
GluI GCNGC 5 cut(s) 270, 612, 752, 1068, 1118
GsuI CTGGAG 4 cut(s) 731, 738, 884, 1411
HaeIII GGCC 4 cut(s) 5, 332, 614, 972
HapII CCGG 3 cut(s) 84, 243, 1399
HindIII AAGCTT 2 cut(s) 413, 1205
HinfI GANTC 8 cut(s) 523, 685, 814, 895, 902, 1007, 1088, 1395
HpaII CCGG 3 cut(s) 84, 243, 1399
HphI GGTGA 3 cut(s) 12, 866, 1003
Hpy166II GTNNAC 3 cut(s) 554, 704, 1092
Hpy188I TCNGA 4 cut(s) 127, 147, 522, 528
Hpy188III TCNNGA 7 cut(s) 409, 581, 689, 712, 899, 1101, 1399
Hpy8I GTNNAC 3 cut(s) 554, 704, 1092
Hpy99I CGWCG 1 cut(s) 1316
HpyAV CCTTC 5 cut(s) 415, 542, 583, 1169, 1266
HpyCH4III ACNGT 3 cut(s) 510, 890, 1096
HpyCH4IV ACGT 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 1204
HpyF3I CTNAG 5 cut(s) 110, 228, 282, 763, 999
HpySE526I ACGT 1 cut(s) 706
Kpn2I TCCGGA 1 cut(s) 1398
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
LmnI GCTCC 4 cut(s) 115, 743, 948, 1114
Lsp1109I GCAGC 2 cut(s) 738, 1079
LweI GCATC 4 cut(s) 245, 1115, 1125, 1266
MaeI CTAG 1 cut(s) 1229
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 3 cut(s) 18, 25, 1307
MalI GATC 8 cut(s) 61, 149, 198, 359, 428, 486, 873, 1043
MbiI CCGCTC 1 cut(s) 1117
MboI GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
MflI RGATCY 2 cut(s) 59, 871
MhlI GDGCHC 2 cut(s) 172, 399
MluCI AATT 3 cut(s) 369, 567, 983
MlyI GAGTC 3 cut(s) 904, 1016, 1097
MmeI TCCRAC 1 cut(s) 175
MroI TCCGGA 1 cut(s) 1398
MroXI GAANNNNTTC 1 cut(s) 369
MseI TTAA 7 cut(s) 336, 378, 533, 956, 986, 1223, 1326
MslI CAYNNNNRTG 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 3 cut(s) 84, 243, 1399
MspR9I CCNGG 5 cut(s) 51, 84, 318, 616, 1390
Mva1269I GAATGC 3 cut(s) 306, 576, 754
MvaI CCWGG 4 cut(s) 51, 318, 616, 1390
MwoI GCNNNNNNNGC 2 cut(s) 115, 1204
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 2 cut(s) 453, 1267
NdeI CATATG 1 cut(s) 421
NdeII GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
NlaIV GGNNCC 1 cut(s) 971
NmuCI GTSAC 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 580
PctI GAATGC 3 cut(s) 306, 576, 754
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 5 cut(s) 523, 685, 814, 902, 1395
PkrI GCNGC 5 cut(s) 271, 613, 753, 1069, 1119
Ple19I CGATCG 1 cut(s) 360
PleI GAGTC 3 cut(s) 903, 1015, 1096
PpsI GAGTC 3 cut(s) 903, 1015, 1096
Ppu21I YACGTR 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 986
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 4 cut(s) 49, 316, 614, 1388
PspEI GGTNACC 1 cut(s) 18
PspGI CCWGG 4 cut(s) 49, 316, 614, 1388
PspN4I GGNNCC 1 cut(s) 971
PspPI GGNCC 5 cut(s) 4, 331, 472, 551, 970
PspXI VCTCGAGB 1 cut(s) 215
PsuI RGATCY 2 cut(s) 59, 871
PvuI CGATCG 1 cut(s) 360
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 2 cut(s) 44, 744
RsaNI GTAC 2 cut(s) 43, 743
RseI CAYNNNNRTG 1 cut(s) 1062
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 7 cut(s) 336, 378, 533, 956, 986, 1223, 1326
SatI GCNGC 5 cut(s) 270, 612, 752, 1068, 1118
Sau3AI GATC 8 cut(s) 59, 147, 196, 357, 426, 484, 871, 1041
Sau96I GGNCC 5 cut(s) 4, 331, 472, 551, 970
SchI GAGTC 3 cut(s) 904, 1016, 1097
ScrFI CCNGG 5 cut(s) 51, 84, 318, 616, 1390
SduI GDGCHC 2 cut(s) 172, 399
SfaNI GCATC 4 cut(s) 245, 1115, 1125, 1266
SfcI CTRYAG 2 cut(s) 666, 1092
Sfr274I CTCGAG 1 cut(s) 215
SinI GGWCC 2 cut(s) 472, 551
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 1062
SmlI CTYRAG 2 cut(s) 215, 1208
SmoI CTYRAG 2 cut(s) 215, 1208
Sse9I AATT 3 cut(s) 369, 567, 983
SsiI CCGC 4 cut(s) 269, 611, 917, 1117
SspMI CTAG 1 cut(s) 1229
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 5 cut(s) 49, 82, 316, 614, 1388
StyI CCWWGG 4 cut(s) 71, 104, 453, 1267
TaaI ACNGT 3 cut(s) 510, 890, 1096
TaiI ACGT 1 cut(s) 709
TaqI TCGA 3 cut(s) 216, 356, 1010
TaqII GACCGA 1 cut(s) 312
TasI AATT 3 cut(s) 369, 567, 983
TatI WGTACW 1 cut(s) 42
TauI GCSGC 3 cut(s) 272, 614, 1120
TfiI GAWTC 5 cut(s) 523, 685, 814, 902, 1395
Tru1I TTAA 7 cut(s) 336, 378, 533, 956, 986, 1223, 1326
Tru9I TTAA 7 cut(s) 336, 378, 533, 956, 986, 1223, 1326
TscAI CASTG 4 cut(s) 286, 515, 886, 1059
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 2 cut(s) 751, 1067
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 7 cut(s) 21, 408, 479, 483, 569, 597, 797
TspGWI ACGGA 1 cut(s) 730
TspRI CASTG 4 cut(s) 286, 515, 886, 1059
VpaK11BI GGWCC 2 cut(s) 472, 551
VspI ATTAAT 1 cut(s) 986
XagI CCTNNNNNAGG 1 cut(s) 54
XapI RAATTY 1 cut(s) 369
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 1091
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 1 cut(s) 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.