Rw0G019990

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00902
OG0002137
Physical Location & Seq
Reverse (-)
54798 .. 56210
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019990.1

Sequence Viewer

Length: 1413 bp
ATGGCGCAAGAGGACGGATCACCTGTAACTTCATCAGCCATGTACTTTCCTGGTTTAGGATCTCCATACCCTTGGCTTTCCCCTGGTATAGGGTCTCCATACCCTTGGCTGAGGGAGCTAAAATCTGAAGAGCGAGGTTTATGTCTGATCCAACTTCTTCAATGCTGTGCTCATCATGTTGCTTCTGGTAGTATTGAGCAGGCAAATGTTTGGCTCGAGCAGATTTCTCAGCTCGCCTCTGCTGGTGGCGATACAATGCAGCGAATAGCGGCTTATTTCACTGAGGCACTTGCAGACCGAATGCTGAAAGGTTTGCCAGGTCTATATAAGGCCCTTAACTCAACGAAACTATCATCAATCGCGGAAGAAATTCACATTAAAAGGCTTTTCTTTGATCTCTGTCCCTTCTTAAAGCTTTCATATGTGATAACAAATCAGGCCATAGTGGAAGCTATGGAAGGGGAGAAGATGGTTCATATTATTGATCTTCATTCATTTGAACCTGCCCAGTGGATAAATCTGATTCAGACATTAAATGCAAGACCTGAAGGTCCACCACATTTGAGAATTACAGGCATTCATGAACATAAGGAGGTGTTGGAGCAAATGGCCGCCAGGTTGACAGAAGAAGCTGAAAAACATGACATTCCCTTTCAGTTCAATGCTATAGTGAGCAAGTTAGAGAATCTTGATGTCGAAAGTTTACGTGTCAAGACTGGAGAAGCACTTGCAGTCTGCTCTGTACTCCAGCTGCATTCACTCTTAGCAACCGATGATGACATGACCAGAAGGAAATCTCCATTAGCAACAAATAACCTGCAAAAAGTCATGCATATCAATAAACTCACTTTAGGTGAGTGGCTGGAGAAAGATCCGATCAGTGCATATAGCCTGAGTCCGGATTCTTCATTATCCCCGCTATCTTTAGGTGCTTCCCCAAAGATGGGGAGTTTTTTAACTGCTCTTTGGGGCCTTTCCCCGAAATTAATGGTGATAACTGAGCAGGAATCAAACCACAATGGTCATACACTGATGGACAGGATCATAGAAGCACTGCACTACTATGCAGCACTTTTTGATTGCTTGGAGTCTACAGTATCGAGAGCATCAATGGAGCGGCAAAAGGTTGAGAAGATGCTTTTTGGGGAGGAAATCAAAAACATCATATCATGTGAAGGAACTGACAGAAAGGAGAGGCATGAGAAGCTTGAGAAGTGGATTTTAAGGCTAGAGTTGTCTGGCTTTGGGAGGGTGCAGTTGAGCTACCATGGAAGGATGCAGGCGAAGAAGATGTTGCGTGACTATGGCTACGATGGCTATGAGATTAAAGAAGACAATGGATGTTTGGTTATTTGCTGGCATGACAGACCACTTTTTTCGGTGTCAGCCTGGAGATTCCGGAGATACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

470

Amino Acids

53.17

Weight (kDa)

5.97

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 50 - 465 3.4e-122 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 549
Acc36I ACCTGC 2 cut(s) 511, 825
AccBSI CCGCTC 1 cut(s) 1117
AccI GTMKAC 1 cut(s) 1091
AccII CGCG 1 cut(s) 362
AccIII TCCGGA 2 cut(s) 898, 1398
AciI CCGC 5 cut(s) 269, 362, 612, 917, 1117
AclWI GGATC 5 cut(s) 25, 67, 142, 866, 1049
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 1 cut(s) 369
AcuI CTGAAG 2 cut(s) 147, 567
AfaI GTAC 2 cut(s) 44, 744
AfiI CCNNNNNNNGG 5 cut(s) 56, 89, 898, 943, 944
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 3 cut(s) 161, 500, 661
AjnI CCWGG 5 cut(s) 49, 82, 316, 614, 1388
AluBI AGCT 8 cut(s) 118, 232, 415, 452, 632, 751, 1207, 1263
AluI AGCT 8 cut(s) 118, 232, 415, 452, 632, 751, 1207, 1263
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 5 cut(s) 25, 67, 142, 866, 1049
Ama87I CYCGRG 1 cut(s) 215
Aor13HI TCCGGA 2 cut(s) 898, 1398
AoxI GGCC 4 cut(s) 330, 438, 609, 970
ApeKI GCWGC 3 cut(s) 259, 751, 1067
ApoI RAATTY 1 cut(s) 369
AseI ATTAAT 1 cut(s) 986
Asp700I GAANNNNTTC 1 cut(s) 369
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 331, 551, 970
AsuHPI GGTGA 3 cut(s) 12, 866, 1003
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 551
BbsI GAAGAC 1 cut(s) 1338
Bbv12I GWGCWC 1 cut(s) 172
BbvCI CCTCAGC 1 cut(s) 110
BbvI GCAGC 3 cut(s) 271, 738, 1079
BccI CCATC 4 cut(s) 463, 937, 1027, 1307
BciT130I CCWGG 5 cut(s) 51, 84, 318, 616, 1390
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 1 cut(s) 1229
BfmI CTRYAG 2 cut(s) 666, 1092
BfuAI ACCTGC 2 cut(s) 511, 825
BisI GCNGC 6 cut(s) 260, 270, 612, 752, 1068, 1118
BlsI GCNGC 6 cut(s) 261, 271, 613, 753, 1069, 1119
Bme1390I CCNGG 5 cut(s) 51, 84, 318, 616, 1390
Bme18I GGWCC 1 cut(s) 551
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 3 cut(s) 331, 551, 970
BmiI GGNNCC 1 cut(s) 971
BmrFI CCNGG 5 cut(s) 51, 84, 318, 616, 1390
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 3 cut(s) 1115, 1125, 1266
BmuI ACTGGG 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 1338
BpmI CTGGAG 4 cut(s) 731, 738, 884, 1411
Bpu10I CCTNAGC 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 1229
BsaAI YACGTR 1 cut(s) 707
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 4 cut(s) 71, 82, 104, 1267
BsaWI WCCGGW 2 cut(s) 898, 1398
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 89, 898, 943, 944
Bse1I ACTGG 2 cut(s) 508, 721
BseAI TCCGGA 2 cut(s) 898, 1398
BseBI CCWGG 5 cut(s) 51, 84, 318, 616, 1390
BseDI CCNNGG 4 cut(s) 71, 82, 104, 1267
BseGI GGATG 2 cut(s) 1281, 1346
BseLI CCNNNNNNNGG 5 cut(s) 56, 89, 898, 943, 944
BseMII CTCAG 5 cut(s) 101, 242, 273, 884, 990
BseNI ACTGG 2 cut(s) 508, 721
BseXI GCAGC 3 cut(s) 271, 738, 1079
BsgI GTGCAG 2 cut(s) 1040, 1274
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 4 cut(s) 332, 440, 611, 972
BsiHKAI GWGCWC 1 cut(s) 172
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 2 cut(s) 899, 1399
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 5 cut(s) 56, 89, 898, 943, 944
BsmAI GTCTC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 3 cut(s) 306, 576, 754
BsnI GGCC 4 cut(s) 332, 440, 611, 972
Bso31I GGTCTC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 172
Bsp13I TCCGGA 2 cut(s) 898, 1398
Bsp143I GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
Bsp19I CCATGG 1 cut(s) 1267
BspACI CCGC 5 cut(s) 269, 362, 612, 917, 1117
BspANI GGCC 4 cut(s) 332, 440, 611, 972
BspCNI CTCAG 5 cut(s) 102, 241, 274, 885, 991
BspEI TCCGGA 2 cut(s) 898, 1398
BspFNI CGCG 1 cut(s) 362
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 971
BspMI ACCTGC 2 cut(s) 511, 825
BspPI GGATC 5 cut(s) 25, 67, 142, 866, 1049
BspQI GCTCTTC 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 99
BsrBI CCGCTC 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 508, 721
BssECI CCNNGG 4 cut(s) 71, 82, 104, 1267
BssMI GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
BssT1I CCWWGG 3 cut(s) 71, 104, 1267
Bst2UI CCWGG 5 cut(s) 51, 84, 318, 616, 1390
Bst4CI ACNGT 1 cut(s) 1096
Bst6I CTCTTC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 4 cut(s) 201, 234, 1281, 1358
BstDEI CTNAG 6 cut(s) 110, 228, 282, 763, 893, 999
BstDSI CCRYGG 1 cut(s) 1267
BstENI CCTNNNNNAGG 2 cut(s) 54, 87
BstF5I GGATG 2 cut(s) 1281, 1346
BstFNI CGCG 1 cut(s) 362
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 8 cut(s) 20, 62, 150, 397, 487, 874, 879, 1044
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
BstMWI GCNNNNNNNGC 3 cut(s) 115, 1204, 1314
BstNI CCWGG 5 cut(s) 51, 84, 318, 616, 1390
BstSCI CCNGG 5 cut(s) 49, 82, 316, 614, 1388
BstSFI CTRYAG 2 cut(s) 666, 1092
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 3 cut(s) 271, 738, 1079
BstV2I GAAGAC 1 cut(s) 1338
BstX2I RGATCY 2 cut(s) 59, 871
BstXI CCANNNNNNTGG 2 cut(s) 72, 105
BstYI RGATCY 2 cut(s) 59, 871
BsuRI GGCC 4 cut(s) 332, 440, 611, 972
BtgI CCRYGG 1 cut(s) 1267
BtsCI GGATG 2 cut(s) 1281, 1346
BtsI GCAGTG 1 cut(s) 1052
BtsIMutI CAGTG 5 cut(s) 279, 515, 886, 1028, 1052
BveI ACCTGC 2 cut(s) 511, 825
Cac8I GCNNGC 4 cut(s) 201, 234, 1281, 1358
CciI TCATGA 1 cut(s) 580
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 331, 551, 970
Csp6I GTAC 2 cut(s) 43, 743
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviQI GTAC 2 cut(s) 43, 743
DdeI CTNAG 6 cut(s) 110, 228, 282, 763, 893, 999
DpnI GATC 8 cut(s) 19, 61, 149, 396, 486, 873, 878, 1043
DpnII GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
DrdI GACNNNNNNGTC 1 cut(s) 549
DseDI GACNNNNNNGTC 1 cut(s) 549
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 3 cut(s) 71, 104, 1267
Eco31I GGTCTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 551
Eco57I CTGAAG 2 cut(s) 147, 567
Eco88I CYCGRG 1 cut(s) 215
EcoNI CCTNNNNNAGG 2 cut(s) 54, 87
EcoO109I RGGNCCY 2 cut(s) 331, 970
EcoRII CCWGG 5 cut(s) 49, 82, 316, 614, 1388
EcoT14I CCWWGG 3 cut(s) 71, 104, 1267
EcoT22I ATGCAT 1 cut(s) 834
ErhI CCWWGG 3 cut(s) 71, 104, 1267
FaqI GGGAC 1 cut(s) 387
FauI CCCGC 1 cut(s) 924
FauNDI CATATG 1 cut(s) 421
FblI GTMKAC 1 cut(s) 1091
Fnu4HI GCNGC 6 cut(s) 260, 270, 612, 752, 1068, 1118
FokI GGATG 2 cut(s) 1288, 1353
Fsp4HI GCNGC 6 cut(s) 260, 270, 612, 752, 1068, 1118
FspBI CTAG 1 cut(s) 1229
GlaI GCGC 1 cut(s) 6
GluI GCNGC 6 cut(s) 260, 270, 612, 752, 1068, 1118
GsuI CTGGAG 4 cut(s) 731, 738, 884, 1411
HaeIII GGCC 4 cut(s) 332, 440, 611, 972
HapII CCGG 2 cut(s) 899, 1399
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 2 cut(s) 413, 1205
HinfI GANTC 7 cut(s) 523, 685, 895, 902, 1007, 1088, 1395
HpaII CCGG 2 cut(s) 899, 1399
HphI GGTGA 3 cut(s) 12, 866, 1003
Hpy166II GTNNAC 4 cut(s) 554, 621, 704, 1092
Hpy188I TCNGA 5 cut(s) 127, 147, 522, 528, 876
Hpy188III TCNNGA 6 cut(s) 581, 689, 712, 899, 1101, 1399
Hpy8I GTNNAC 4 cut(s) 554, 621, 704, 1092
HpyAV CCTTC 6 cut(s) 415, 452, 542, 783, 1169, 1266
HpyCH4III ACNGT 1 cut(s) 1096
HpyCH4IV ACGT 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 1204, 1314
HpyF3I CTNAG 6 cut(s) 110, 228, 282, 763, 893, 999
HpySE526I ACGT 1 cut(s) 706
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 2 cut(s) 898, 1398
Kzo9I GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 3 cut(s) 115, 601, 1114
Lsp1109I GCAGC 3 cut(s) 271, 738, 1079
LweI GCATC 3 cut(s) 1115, 1125, 1266
MaeI CTAG 1 cut(s) 1229
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 25, 1298
MalI GATC 8 cut(s) 19, 61, 149, 396, 486, 873, 878, 1043
MbiI CCGCTC 1 cut(s) 1117
MboI GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
MflI RGATCY 2 cut(s) 59, 871
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 3 cut(s) 369, 567, 983
MlyI GAGTC 2 cut(s) 904, 1097
MmeI TCCRAC 2 cut(s) 175, 579
MnlI CCTC 9 cut(s) 4, 105, 128, 247, 277, 586, 1141, 1188, 1242
Mph1103I ATGCAT 1 cut(s) 834
MroI TCCGGA 2 cut(s) 898, 1398
MroXI GAANNNNTTC 1 cut(s) 369
MseI TTAA 8 cut(s) 336, 378, 410, 533, 956, 986, 1223, 1326
MslI CAYNNNNRTG 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 2 cut(s) 899, 1399
MspR9I CCNGG 5 cut(s) 51, 84, 318, 616, 1390
Mva1269I GAATGC 3 cut(s) 306, 576, 754
MvaI CCWGG 5 cut(s) 51, 84, 318, 616, 1390
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 115, 1204, 1314
NcoI CCATGG 1 cut(s) 1267
NdeI CATATG 1 cut(s) 421
NdeII GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
NlaIV GGNNCC 1 cut(s) 971
NmuCI GTSAC 1 cut(s) 1298
NsiI ATGCAT 1 cut(s) 834
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 123
PctI GAATGC 3 cut(s) 306, 576, 754
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 5 cut(s) 523, 685, 902, 1007, 1395
PkrI GCNGC 6 cut(s) 261, 271, 613, 753, 1069, 1119
PleI GAGTC 2 cut(s) 903, 1096
PpsI GAGTC 2 cut(s) 903, 1096
Ppu21I YACGTR 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 986
Psp6I CCWGG 5 cut(s) 49, 82, 316, 614, 1388
PspGI CCWGG 5 cut(s) 49, 82, 316, 614, 1388
PspN4I GGNNCC 1 cut(s) 971
PspPI GGNCC 3 cut(s) 331, 551, 970
PspXI VCTCGAGB 1 cut(s) 215
PsuI RGATCY 2 cut(s) 59, 871
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 2 cut(s) 44, 744
RsaNI GTAC 2 cut(s) 43, 743
RseI CAYNNNNRTG 1 cut(s) 1062
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 8 cut(s) 336, 378, 410, 533, 956, 986, 1223, 1326
SatI GCNGC 6 cut(s) 260, 270, 612, 752, 1068, 1118
Sau3AI GATC 8 cut(s) 17, 59, 147, 394, 484, 871, 876, 1041
Sau96I GGNCC 3 cut(s) 331, 551, 970
SchI GAGTC 2 cut(s) 904, 1097
ScrFI CCNGG 5 cut(s) 51, 84, 318, 616, 1390
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 1115, 1125, 1266
SfcI CTRYAG 2 cut(s) 666, 1092
Sfr274I CTCGAG 1 cut(s) 215
SinI GGWCC 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 1062
SmlI CTYRAG 2 cut(s) 215, 1208
SmoI CTYRAG 2 cut(s) 215, 1208
Sse9I AATT 3 cut(s) 369, 567, 983
SsiI CCGC 5 cut(s) 269, 362, 612, 917, 1117
SspMI CTAG 1 cut(s) 1229
StyD4I CCNGG 5 cut(s) 49, 82, 316, 614, 1388
StyI CCWWGG 3 cut(s) 71, 104, 1267
TaaI ACNGT 1 cut(s) 1096
TaiI ACGT 1 cut(s) 709
TaqI TCGA 3 cut(s) 216, 696, 1100
TaqII GACCGA 1 cut(s) 312
TasI AATT 3 cut(s) 369, 567, 983
TatI WGTACW 2 cut(s) 42, 742
TauI GCSGC 3 cut(s) 272, 614, 1120
TfiI GAWTC 5 cut(s) 523, 685, 902, 1007, 1395
Tru1I TTAA 8 cut(s) 336, 378, 410, 533, 956, 986, 1223, 1326
Tru9I TTAA 8 cut(s) 336, 378, 410, 533, 956, 986, 1223, 1326
TscAI CASTG 5 cut(s) 286, 515, 886, 1035, 1059
TseFI GTSAC 1 cut(s) 1298
TseI GCWGC 3 cut(s) 259, 751, 1067
Tsp45I GTSAC 1 cut(s) 1298
TspDTI ATGAA 8 cut(s) 21, 408, 464, 479, 483, 569, 597, 897
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 5 cut(s) 286, 515, 886, 1035, 1059
VpaK11BI GGWCC 1 cut(s) 551
VspI ATTAAT 1 cut(s) 986
XagI CCTNNNNNAGG 2 cut(s) 54, 87
XapI RAATTY 1 cut(s) 369
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 1091
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 1 cut(s) 1229
Zsp2I ATGCAT 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.