Rh5DG359600

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002137
Physical Location & Seq
Forward (+)
53657944 .. 53660670
2727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG359600.1

Sequence Viewer

Length: 1353 bp
ATGACGTCACCTAGTTTAGGATCACCATACCCTTGGCAAAGAGAACTCAAATCGGAGGAGCGGGGTCTGTGTTTGATCCACTTGTTGCTCACTTGTGCAAATTATGTTGCTGCGGGAAACCTCGAAAATGCAAATATTGCCCTTGAGCAAATCTCCCTGCTTGCCTCTTCGGATGGTGATACCATGCAGCGGATTGCAGCCTACTTCACTGAGGCTCTTGCTGACCGAATCCTCAAAGCTTGGCCTGGTCTTTATAAGGCCCTATATTCCACTAGAATATCTTCAGTTTCTGAAGAATTTCTAGTTCGGAAAGTGTTCTTTGAGATGCTTCCATTCCTAAAGGTGGCTTATGTGCTCACAAACCAAGCTATTATTGAAGCGATGGAAGAAGAAAAGATGGTCCATATAATTGACCTTAATTCATCTGAACCTGCACAGTGGATAGCTCTTCTTCATGTTTTAAGTGCAAGGCCTGAGGGTCCACCTCATTTGAGAATTACCGGTGTCCATCAACGAAAAGAAGTACTAGATCAAATGGCTCATAGCTTGACTGAGGAAGCAGAAAGATTGGATACTCCATTTCAGTTCAATCCCGTAGTCAGCAAGTTGGAAACACTTGATTTTGAAAAGCTCCATGTTAAAACCGGTGAGGCTTTAGCTATCAGCTCAGTTCTCCAATTTCACTCCCTCTTGTCATCTGATGATGATTTCCACAGAAAGAAATTCTCATTGGAATCAAAGAATCCAAATAGAGTTCACTCACCTGGAGTGTTGCAAATGGGCCAAGGCACATTGGGTGAGTTGCTAGAGAAAGATATGGGCAAGGGATATAGCCCCAGTCCTGACTCAACCTCGTCATCCCCACTATCCCCAGTTGGTTCTGTGAAGATGGACAGCTTTCTTAATGCATTTTGGGGTTTGACACCGAAGATCATGGTAGTTACCGAACAAGATTCCAACCATAATGGCTCCACTCTAATGGAGAGGCTATTCGAAGCTTTATACTCGTATGCCGCATTATTTGATTGCTTGGAGTCTACGATGTCAAGAACTTCATTGGAAAGAATGAAGGTAGAGAAAATGCTATTTGGGGAGGAAATTAAAAACATTATAGCTTGTGAAGGATCTGAGAGGAAAGAAAGACATGAAAAACTTGAGAAGTGGATTCAAAGGCTCGATTTAGCTGGCTTTGGAAATGTACCTTTGAGCTATTATGGAATGCTTCAGGCAAGGAAGTTGTTGCAGGGTTATGGTTGTGATGGATATAGGATCAGGGAAGAGAATGGCTGTGTGTTGATGTCGTGGCAAGATCGACCCCTATTTTCTGTCTCAGCTTGGAGATGCAGGAAGTAG

Protein Analysis

450

Amino Acids

50.81

Weight (kDa)

5.82

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 25 - 447 1.8e-120 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 439
AccBSI CCGCTC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 1037
AciI CCGC 4 cut(s) 61, 113, 190, 1014
AclWI GGATC 4 cut(s) 28, 70, 1132, 1277
AcsI RAATTY 2 cut(s) 296, 722
AcuI CTGAAG 3 cut(s) 267, 312, 1208
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 525, 1200
AfiI CCNNNNNNNGG 3 cut(s) 17, 189, 343
AgeI ACCGGT 2 cut(s) 500, 644
AgsI TTSAA 4 cut(s) 377, 589, 626, 1169
AjnI CCWGG 2 cut(s) 244, 763
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 1333
AlwI GGATC 4 cut(s) 28, 70, 1132, 1277
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 4 cut(s) 242, 258, 470, 781
ApeKI GCWGC 3 cut(s) 110, 187, 197
ApoI RAATTY 2 cut(s) 296, 722
AsiGI ACCGGT 2 cut(s) 500, 644
Asp700I GAANNNNTTC 3 cut(s) 280, 297, 314
AspS9I GGNCC 4 cut(s) 259, 400, 479, 781
AsuHPI GGTGA 5 cut(s) 15, 188, 659, 753, 809
AsuII TTCGAA 1 cut(s) 993
AvaII GGWCC 2 cut(s) 400, 479
AxyI CCTNAGG 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 3 cut(s) 97, 199, 209
BccI CCATC 6 cut(s) 167, 376, 391, 516, 883, 1253
BciT130I CCWGG 2 cut(s) 246, 765
BciVI GTATCC 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 1333
BfaI CTAG 5 cut(s) 12, 273, 302, 527, 806
BfuAI ACCTGC 1 cut(s) 439
BfuI GTATCC 1 cut(s) 565
BisI GCNGC 4 cut(s) 111, 188, 198, 1014
BlsI GCNGC 4 cut(s) 112, 189, 199, 1015
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 2 cut(s) 246, 765
Bme18I GGWCC 2 cut(s) 400, 479
BmgT120I GGNCC 4 cut(s) 259, 400, 479, 781
BmiI GGNNCC 2 cut(s) 480, 970
BmrFI CCNGG 2 cut(s) 246, 765
BmrI ACTGGG 2 cut(s) 831, 866
BmsI GCATC 2 cut(s) 315, 1331
BmuI ACTGGG 2 cut(s) 831, 866
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 993
BpuEI CTTGAG 2 cut(s) 164, 1175
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 32, 784
BsaWI WCCGGW 2 cut(s) 500, 644
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 189, 343
Bse118I RCCGGY 2 cut(s) 500, 644
Bse1I ACTGG 2 cut(s) 837, 872
Bse21I CCTNAGG 1 cut(s) 474
BseBI CCWGG 2 cut(s) 246, 765
BseDI CCNNGG 2 cut(s) 32, 784
BseGI GGATG 2 cut(s) 178, 857
BseLI CCNNNNNNNGG 3 cut(s) 17, 189, 343
BseMII CTCAG 6 cut(s) 201, 465, 543, 681, 1119, 1344
BseNI ACTGG 2 cut(s) 837, 872
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 3 cut(s) 97, 199, 209
BsgI GTGCAG 1 cut(s) 417
BshFI GGCC 4 cut(s) 244, 260, 472, 783
BshTI ACCGGT 2 cut(s) 500, 644
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 2 cut(s) 501, 645
BslI CCNNNNNNNGG 3 cut(s) 17, 189, 343
BsmAI GTCTC 1 cut(s) 1333
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 4 cut(s) 244, 260, 472, 783
Bsp119I TTCGAA 1 cut(s) 993
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
BspACI CCGC 4 cut(s) 61, 113, 190, 1014
BspANI GGCC 4 cut(s) 244, 260, 472, 783
BspCNI CTCAG 6 cut(s) 202, 466, 544, 680, 1120, 1343
BspLI GGNNCC 2 cut(s) 480, 970
BspMI ACCTGC 1 cut(s) 439
BspPI GGATC 4 cut(s) 28, 70, 1132, 1277
BspQI GCTCTTC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 993
BsrBI CCGCTC 1 cut(s) 61
BsrFI RCCGGY 2 cut(s) 500, 644
BsrI ACTGG 2 cut(s) 837, 872
BssAI RCCGGY 2 cut(s) 500, 644
BssECI CCNNGG 2 cut(s) 32, 784
BssMI GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 32, 784
Bst2UI CCWGG 2 cut(s) 246, 765
Bst4CI ACNGT 1 cut(s) 438
Bst6I CTCTTC 3 cut(s) 172, 453, 1272
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 993
BstC8I GCNNGC 2 cut(s) 162, 1186
BstDEI CTNAG 6 cut(s) 210, 474, 552, 667, 1128, 1330
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 178, 857
BstKTI GATC 7 cut(s) 23, 78, 532, 933, 1127, 1272, 1312
BstMAI GTCTC 1 cut(s) 1333
BstMBI GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 2 cut(s) 246, 765
BstSCI CCNGG 2 cut(s) 244, 763
BstV1I GCAGC 3 cut(s) 97, 199, 209
BstX2I RGATCY 1 cut(s) 1124
BstXI CCANNNNNNTGG 2 cut(s) 33, 979
BstYI RGATCY 1 cut(s) 1124
Bsu36I CCTNAGG 1 cut(s) 474
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 4 cut(s) 244, 260, 472, 783
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 2 cut(s) 178, 857
BtsIMutI CAGTG 2 cut(s) 207, 443
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 2 cut(s) 162, 1186
CaiI CAGNNNCTG 1 cut(s) 290
Cfr10I RCCGGY 2 cut(s) 500, 644
Cfr13I GGNCC 4 cut(s) 259, 400, 479, 781
Csp6I GTAC 2 cut(s) 524, 1199
CspAI ACCGGT 2 cut(s) 500, 644
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviAII CATG 5 cut(s) 184, 455, 635, 934, 1145
CviQI GTAC 2 cut(s) 524, 1199
DdeI CTNAG 6 cut(s) 210, 474, 552, 667, 1128, 1330
DpnI GATC 7 cut(s) 22, 77, 531, 932, 1126, 1271, 1311
DpnII GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
Eam1104I CTCTTC 3 cut(s) 172, 453, 1272
EarI CTCTTC 3 cut(s) 172, 453, 1272
Eco130I CCWWGG 2 cut(s) 32, 784
Eco147I AGGCCT 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 400, 479
Eco57I CTGAAG 3 cut(s) 267, 312, 1208
Eco81I CCTNAGG 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 244, 763
EcoT14I CCWWGG 2 cut(s) 32, 784
EcoT22I ATGCAT 1 cut(s) 910
ErhI CCWWGG 2 cut(s) 32, 784
FaeI CATG 5 cut(s) 187, 458, 638, 937, 1148
FatI CATG 5 cut(s) 183, 454, 634, 933, 1144
FauI CCCGC 2 cut(s) 54, 106
FblI GTMKAC 1 cut(s) 1037
Fnu4HI GCNGC 4 cut(s) 111, 188, 198, 1014
FokI GGATG 2 cut(s) 185, 844
Fsp4HI GCNGC 4 cut(s) 111, 188, 198, 1014
FspBI CTAG 5 cut(s) 12, 273, 302, 527, 806
GluI GCNGC 4 cut(s) 111, 188, 198, 1014
GsuI CTGGAG 1 cut(s) 786
HaeIII GGCC 4 cut(s) 244, 260, 472, 783
HapII CCGG 2 cut(s) 501, 645
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 187, 458, 638, 937, 1148
HindIII AAGCTT 2 cut(s) 237, 996
HinfI GANTC 7 cut(s) 228, 734, 742, 845, 953, 1034, 1165
HpaII CCGG 2 cut(s) 501, 645
HphI GGTGA 5 cut(s) 15, 188, 659, 753, 809
Hpy166II GTNNAC 3 cut(s) 482, 757, 1038
Hpy188I TCNGA 7 cut(s) 55, 172, 292, 309, 427, 700, 1129
Hpy188III TCNNGA 2 cut(s) 842, 1047
Hpy8I GTNNAC 3 cut(s) 482, 757, 1038
HpyAV CCTTC 2 cut(s) 1063, 1115
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4IV ACGT 1 cut(s) 5
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 6 cut(s) 210, 474, 552, 667, 1128, 1330
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 187, 458, 638, 937, 1148
Kzo9I GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 3 cut(s) 58, 636, 974
Lsp1109I GCAGC 3 cut(s) 97, 199, 209
LweI GCATC 2 cut(s) 315, 1331
MaeI CTAG 5 cut(s) 12, 273, 302, 527, 806
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 6, 940
MalI GATC 7 cut(s) 22, 77, 531, 932, 1126, 1271, 1311
MbiI CCGCTC 1 cut(s) 61
MboI GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
MflI RGATCY 1 cut(s) 1124
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 8 cut(s) 100, 296, 408, 418, 495, 677, 722, 1098
MlyI GAGTC 2 cut(s) 839, 1043
MmeI TCCRAC 2 cut(s) 588, 981
Mph1103I ATGCAT 1 cut(s) 910
MroXI GAANNNNTTC 3 cut(s) 280, 297, 314
MseI TTAA 5 cut(s) 417, 461, 639, 903, 1101
MslI CAYNNNNRTG 1 cut(s) 977
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 2 cut(s) 501, 645
MspR9I CCNGG 2 cut(s) 246, 765
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 2 cut(s) 246, 765
MwoI GCNNNNNNNGC 1 cut(s) 137
NdeII GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
NlaIII CATG 5 cut(s) 187, 458, 638, 937, 1148
NlaIV GGNNCC 2 cut(s) 480, 970
NmuCI GTSAC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 910
NspV TTCGAA 1 cut(s) 993
PceI AGGCCT 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 1 cut(s) 1224
PdmI GAANNNNTTC 3 cut(s) 280, 297, 314
PfeI GAWTC 5 cut(s) 228, 734, 742, 953, 1165
PinAI ACCGGT 2 cut(s) 500, 644
PkrI GCNGC 4 cut(s) 112, 189, 199, 1015
PleI GAGTC 2 cut(s) 839, 1042
PpsI GAGTC 2 cut(s) 839, 1042
PsiI TTATAA 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 244, 763
PspGI CCWGG 2 cut(s) 244, 763
PspN4I GGNNCC 2 cut(s) 480, 970
PspPI GGNCC 4 cut(s) 259, 400, 479, 781
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 1124
RsaI GTAC 2 cut(s) 525, 1200
RsaNI GTAC 2 cut(s) 524, 1199
RseI CAYNNNNRTG 1 cut(s) 977
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 5 cut(s) 417, 461, 639, 903, 1101
SatI GCNGC 4 cut(s) 111, 188, 198, 1014
Sau3AI GATC 7 cut(s) 20, 75, 529, 930, 1124, 1269, 1309
Sau96I GGNCC 4 cut(s) 259, 400, 479, 781
ScaI AGTACT 1 cut(s) 525
SchI GAGTC 2 cut(s) 839, 1043
ScrFI CCNGG 2 cut(s) 246, 765
SduI GDGCHC 1 cut(s) 357
SfaNI GCATC 2 cut(s) 315, 1331
SfuI TTCGAA 1 cut(s) 993
SinI GGWCC 2 cut(s) 400, 479
SmiMI CAYNNNNRTG 1 cut(s) 977
SmlI CTYRAG 2 cut(s) 143, 1154
SmoI CTYRAG 2 cut(s) 143, 1154
Sse9I AATT 8 cut(s) 100, 296, 408, 418, 495, 677, 722, 1098
SseBI AGGCCT 1 cut(s) 472
SsiI CCGC 4 cut(s) 61, 113, 190, 1014
SspI AATATT 1 cut(s) 136
SspMI CTAG 5 cut(s) 12, 273, 302, 527, 806
StuI AGGCCT 1 cut(s) 472
StyD4I CCNGG 2 cut(s) 244, 763
StyI CCWWGG 2 cut(s) 32, 784
TaaI ACNGT 1 cut(s) 438
TaiI ACGT 1 cut(s) 8
TaqI TCGA 4 cut(s) 123, 993, 1176, 1312
TaqII GACCGA 1 cut(s) 240
TasI AATT 8 cut(s) 100, 296, 408, 418, 495, 677, 722, 1098
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 1016
TfiI GAWTC 5 cut(s) 228, 734, 742, 953, 1165
Tru1I TTAA 5 cut(s) 417, 461, 639, 903, 1101
Tru9I TTAA 5 cut(s) 417, 461, 639, 903, 1101
TscAI CASTG 2 cut(s) 214, 443
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 3 cut(s) 110, 187, 197
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 5 cut(s) 411, 443, 1044, 1082, 1161
TspRI CASTG 2 cut(s) 214, 443
VpaK11BI GGWCC 2 cut(s) 400, 479
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 2 cut(s) 296, 722
XmiI GTMKAC 1 cut(s) 1037
XmnI GAANNNNTTC 3 cut(s) 280, 297, 314
XspI CTAG 5 cut(s) 12, 273, 302, 527, 806
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.