MD02G1295800.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002137
Physical Location & Seq
Reverse (-)
35009786 .. 35012501
2716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1295800.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGCACAAGAGGACGGGTCACCGGTAACTTCATCAGCGCTGCAGTTCTTTCCCTGGCTATCTCCTGGGATAGGATCTCCATACTCACCATGGCTCAGGGAGCTAAAATCTGAGGAGAGAGGTTTATGCCTGATCCAGCTTCTGTACGCCTGTGCTAGCCATGTCGCATCAGGTAGCATCGAGAATGCAAATATTTGGCTTGACCAGACTTGTCAGCTCGCCTCTCCTGATGGTGATTCAATGCAGAGAATAGCTGCTTATTTCACCGAGGCACTTGCAGACCGGATGCTTAAAGGTTTGCCCGGTCTGCATAAAGCGCTTAATTCAACAAAAATATCATCAGTCTCGGAAGAAATTCTTGTTCAAAGGCTGTTCTTTGATCTCTGTCCTTTCCTCAAGGTTGCGTATGTGGTCACAAATCAGGCCATAGTGGAAGCCATGGAAGGGGAGAAGATGGTTCATATTATCGATCTTCATTCATGTGAGCCTGCCCAGTGGATTAATCTGATTCAGACATTAAATGCACGACCTGAAGGCCCCCCTCATTTGAGAATTACTGGCATTCATGAACAAAAAGAAGTATTAGACCAAATGTTTCTTCGGTTGACAGAAGAAGCTGAAAATCTGAACATCCCATTTCAGTTCAATGCTATAGTTAGCAAGCTAGAGAACCTTGATATCGAAAGTTTGCGTGTCAAGACTGGGGAGGCTCTGGCAGTCTGCTCCGTACTGCAGCTACATTCTCTCTTGGCAAGTGATGATGATCATAGGAGCAAAACACCATTAGCATCTAAGAACCTGCAGAAAGTCTTGCATATGAATAAACTCACTTTAGGAGAGTGGCTGGAGAAAGATCCAATTAACGGCAGTCCCGATTCTGCTTTGTCCCCGCTATCCAGTGCTTCACCAAAGATGGGGAGTTTTTTAACTTCTCTTTGGGGCCTTTCGCCAAAACTAATGGTAATAACAGAGCAAGAGGCAAACCACAATGGCCATACATTGATGGACAGGATCATGGAAGCATTGTACTTTTATGGAGCACTTTTTGATTGCTTAGAGTCTACAGTATCGAGATCACCAATGGAGCGACAGAAGGTTGAGAAGCTGCTTTTCGGAGAGGAAATAAAAAACATCATAGCATGTGAAGGAACTGAGCGAACGGAGAGGCATGAGAAGCTTGAGAAGTGGATTTTAAGGCTTGAGTTGGCTGGGTTTGGCAGGGTGCCTTTGAGCTACAACGGAATGTTGCAGGCTACGGAGCTGTTGCGTGGCTATGACGGGTACAAGATTAAAGAACATAATGGATGTGTGGTTATTTGCTGGCACGACAGACCTCTTTTTTCGATTTCAGGCTGGAGGTTTAGGAGATACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

459

Amino Acids

51.57

Weight (kDa)

5.67

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 44 - 453 3e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 1222
AccI GTMKAC 1 cut(s) 1061
AciI CCGC 1 cut(s) 890
AclWI GGATC 4 cut(s) 82, 127, 848, 1019
AcoI YGGCCR 1 cut(s) 991
AcsI RAATTY 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 4 cut(s) 146, 729, 1028, 1283
AfeI AGCGCT 2 cut(s) 39, 318
AfiI CCNNNNNNNGG 4 cut(s) 71, 444, 863, 914
AgeI ACCGGT 1 cut(s) 22
AgsI TTSAA 4 cut(s) 240, 327, 365, 646
AjnI CCWGG 2 cut(s) 53, 64
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
Alw21I GWGCWC 1 cut(s) 1042
Alw26I GTCTC 1 cut(s) 349
AlwI GGATC 4 cut(s) 82, 127, 848, 1019
AlwNI CAGNNNCTG 1 cut(s) 142
Aor51HI AGCGCT 2 cut(s) 39, 318
AoxI GGCC 4 cut(s) 423, 535, 940, 991
ApeKI GCWGC 4 cut(s) 40, 254, 733, 1105
ApoI RAATTY 1 cut(s) 354
AseI ATTAAT 1 cut(s) 501
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 40, 319
AspS9I GGNCC 2 cut(s) 536, 940
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 6 cut(s) 12, 78, 245, 256, 897, 1068
AsuNHI GCTAGC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 993
BanI GGYRCC 1 cut(s) 1222
Bbv12I GWGCWC 1 cut(s) 1042
BbvI GCAGC 4 cut(s) 27, 241, 745, 1092
BccI CCATC 4 cut(s) 224, 448, 907, 997
BceAI ACGGC 1 cut(s) 880
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 2 cut(s) 55, 66
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 2 cut(s) 156, 665
BfmI CTRYAG 5 cut(s) 41, 651, 731, 800, 1062
BfoI RGCGCY 2 cut(s) 41, 320
BfuAI ACCTGC 1 cut(s) 807
BisI GCNGC 4 cut(s) 41, 255, 734, 1106
BlsI GCNGC 4 cut(s) 42, 256, 735, 1107
Bme1390I CCNGG 3 cut(s) 55, 66, 303
BmgT120I GGNCC 2 cut(s) 536, 940
BmiI GGNNCC 3 cut(s) 538, 941, 1224
BmrFI CCNGG 3 cut(s) 55, 66, 303
BmrI ACTGGG 2 cut(s) 487, 711
BmsI GCATC 4 cut(s) 176, 186, 276, 797
BmtI GCTAGC 1 cut(s) 159
BmuI ACTGGG 2 cut(s) 487, 711
BpmI CTGGAG 2 cut(s) 866, 1375
Bpu10I CCTNAGC 1 cut(s) 95
BpuEI CTTGAG 3 cut(s) 380, 1199, 1220
BpuMI CCSGG 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 5 cut(s) 53, 65, 89, 267, 438
BsaWI WCCGGW 2 cut(s) 22, 282
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 444, 863, 914
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 4 cut(s) 493, 562, 706, 897
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 2 cut(s) 55, 66
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 5 cut(s) 53, 65, 89, 267, 438
BseGI GGATG 3 cut(s) 291, 630, 1310
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 4 cut(s) 71, 444, 863, 914
BseMII CTCAG 3 cut(s) 102, 109, 1143
BseNI ACTGG 4 cut(s) 493, 562, 706, 897
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 4 cut(s) 27, 241, 745, 1092
BseYI CCCAGC 1 cut(s) 1208
BshFI GGCC 4 cut(s) 425, 537, 942, 993
BshNI GGYRCC 1 cut(s) 1222
BshTI ACCGGT 1 cut(s) 22
BshVI ATCGAT 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 1042
BsiSI CCGG 3 cut(s) 23, 283, 303
BslFI GGGAC 2 cut(s) 855, 871
BslI CCNNNNNNNGG 4 cut(s) 71, 444, 863, 914
BsmAI GTCTC 1 cut(s) 349
BsmFI GGGAC 2 cut(s) 855, 871
BsmI GAATGC 2 cut(s) 190, 561
BsnI GGCC 4 cut(s) 425, 537, 942, 993
Bsp1286I GDGCHC 1 cut(s) 1042
Bsp143I GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
Bsp19I CCATGG 2 cut(s) 89, 438
BspACI CCGC 1 cut(s) 890
BspANI GGCC 4 cut(s) 425, 537, 942, 993
BspCNI CTCAG 3 cut(s) 103, 108, 1144
BspDI ATCGAT 1 cut(s) 468
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 3 cut(s) 538, 941, 1224
BspMAI CTGCAG 3 cut(s) 45, 735, 804
BspMI ACCTGC 1 cut(s) 807
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 4 cut(s) 82, 127, 848, 1019
BspT107I GGYRCC 1 cut(s) 1222
BsrFI RCCGGY 1 cut(s) 22
BsrI ACTGG 4 cut(s) 493, 562, 706, 897
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 5 cut(s) 53, 65, 89, 267, 438
BssMI GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
BssT1I CCWWGG 2 cut(s) 89, 438
Bst2UI CCWGG 2 cut(s) 55, 66
Bst4CI ACNGT 1 cut(s) 1066
BstC8I GCNNGC 6 cut(s) 157, 219, 489, 662, 1251, 1322
BstDEI CTNAG 5 cut(s) 95, 111, 792, 1054, 1152
BstDSI CCRYGG 2 cut(s) 89, 438
BstEII GGTNACC 1 cut(s) 18
BstENI CCTNNNNNAGG 1 cut(s) 69
BstF5I GGATG 3 cut(s) 291, 630, 1310
BstH2I RGCGCY 2 cut(s) 41, 320
BstHHI GCGC 2 cut(s) 40, 319
BstKTI GATC 8 cut(s) 77, 135, 382, 472, 766, 856, 1014, 1076
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
BstMWI GCNNNNNNNGC 4 cut(s) 100, 307, 316, 1174
BstNI CCWGG 2 cut(s) 55, 66
BstNSI RCATGY 1 cut(s) 1143
BstPI GGTNACC 1 cut(s) 18
BstSCI CCNGG 3 cut(s) 53, 64, 301
BstSFI CTRYAG 5 cut(s) 41, 651, 731, 800, 1062
BstV1I GCAGC 4 cut(s) 27, 241, 745, 1092
BstX2I RGATCY 2 cut(s) 74, 853
BstYI RGATCY 2 cut(s) 74, 853
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 4 cut(s) 425, 537, 942, 993
BsuTUI ATCGAT 1 cut(s) 468
BtgI CCRYGG 2 cut(s) 89, 438
BtsCI GGATG 3 cut(s) 291, 630, 1310
BtsIMutI CAGTG 2 cut(s) 500, 904
BveI ACCTGC 1 cut(s) 807
Cac8I GCNNGC 6 cut(s) 157, 219, 489, 662, 1251, 1322
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 565
CfoI GCGC 2 cut(s) 40, 319
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 2 cut(s) 536, 940
ClaI ATCGAT 1 cut(s) 468
Csp6I GTAC 4 cut(s) 145, 728, 1027, 1282
CspAI ACCGGT 1 cut(s) 22
CviAII CATG 8 cut(s) 90, 161, 439, 480, 566, 1015, 1140, 1169
CviQI GTAC 4 cut(s) 145, 728, 1027, 1282
DdeI CTNAG 5 cut(s) 95, 111, 792, 1054, 1152
DpnI GATC 8 cut(s) 76, 134, 381, 471, 765, 855, 1013, 1075
DpnII GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
EaeI YGGCCR 1 cut(s) 991
Eco130I CCWWGG 2 cut(s) 89, 438
Eco32I GATATC 1 cut(s) 679
Eco47III AGCGCT 2 cut(s) 39, 318
Eco57I CTGAAG 1 cut(s) 552
Eco91I GGTNACC 1 cut(s) 18
EcoNI CCTNNNNNAGG 1 cut(s) 69
EcoO109I RGGNCCY 2 cut(s) 536, 940
EcoO65I GGTNACC 1 cut(s) 18
EcoRII CCWGG 2 cut(s) 53, 64
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 2 cut(s) 89, 438
ErhI CCWWGG 2 cut(s) 89, 438
FaeI CATG 8 cut(s) 93, 164, 442, 483, 569, 1018, 1143, 1172
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FaqI GGGAC 2 cut(s) 855, 871
FatI CATG 8 cut(s) 89, 160, 438, 479, 565, 1014, 1139, 1168
FauI CCCGC 1 cut(s) 897
FauNDI CATATG 1 cut(s) 816
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 1061
Fnu4HI GCNGC 4 cut(s) 41, 255, 734, 1106
FokI GGATG 3 cut(s) 298, 617, 1317
Fsp4HI GCNGC 4 cut(s) 41, 255, 734, 1106
FspBI CTAG 2 cut(s) 156, 665
GlaI GCGC 2 cut(s) 39, 318
GluI GCNGC 4 cut(s) 41, 255, 734, 1106
GsaI CCCAGC 1 cut(s) 1212
GsuI CTGGAG 2 cut(s) 866, 1375
HaeII RGCGCY 2 cut(s) 41, 320
HaeIII GGCC 4 cut(s) 425, 537, 942, 993
HapII CCGG 3 cut(s) 23, 283, 303
HhaI GCGC 2 cut(s) 40, 319
Hin1II CATG 8 cut(s) 93, 164, 442, 483, 569, 1018, 1143, 1172
Hin6I GCGC 2 cut(s) 38, 317
HinP1I GCGC 2 cut(s) 38, 317
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 4 cut(s) 236, 508, 875, 1058
HpaII CCGG 3 cut(s) 23, 283, 303
HphI GGTGA 6 cut(s) 12, 78, 245, 256, 897, 1068
Hpy166II GTNNAC 2 cut(s) 606, 1062
Hpy188I TCNGA 6 cut(s) 112, 349, 507, 513, 627, 1115
Hpy188III TCNNGA 6 cut(s) 181, 227, 566, 697, 872, 1071
Hpy8I GTNNAC 2 cut(s) 606, 1062
HpyAV CCTTC 4 cut(s) 437, 527, 1087, 1139
HpyCH4III ACNGT 1 cut(s) 1066
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 307, 316, 1174
HpyF3I CTNAG 5 cut(s) 95, 111, 792, 1054, 1152
Hsp92II CATG 8 cut(s) 93, 164, 442, 483, 569, 1018, 1143, 1172
HspAI GCGC 2 cut(s) 38, 317
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
LmnI GCTCC 6 cut(s) 100, 728, 771, 1037, 1084, 1258
Lsp1109I GCAGC 4 cut(s) 27, 241, 745, 1092
LweI GCATC 4 cut(s) 176, 186, 276, 797
MaeI CTAG 2 cut(s) 156, 665
MaeIII GTNAC 3 cut(s) 18, 25, 412
MalI GATC 8 cut(s) 76, 134, 381, 471, 765, 855, 1013, 1075
MboI GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
MboII GAAGA 5 cut(s) 362, 463, 464, 590, 623
MflI RGATCY 2 cut(s) 74, 853
MhlI GDGCHC 1 cut(s) 1042
MlsI TGGCCA 1 cut(s) 993
MluCI AATT 4 cut(s) 322, 354, 552, 858
MluNI TGGCCA 1 cut(s) 993
MlyI GAGTC 1 cut(s) 1067
Mox20I TGGCCA 1 cut(s) 993
MroXI GAANNNNTTC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 993
MseI TTAA 8 cut(s) 291, 321, 501, 518, 861, 926, 1193, 1290
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 993
MspI CCGG 3 cut(s) 23, 283, 303
MspR9I CCNGG 3 cut(s) 55, 66, 303
Mva1269I GAATGC 2 cut(s) 190, 561
MvaI CCWGG 2 cut(s) 55, 66
MwoI GCNNNNNNNGC 4 cut(s) 100, 307, 316, 1174
NciI CCSGG 1 cut(s) 303
NcoI CCATGG 2 cut(s) 89, 438
NdeI CATATG 1 cut(s) 816
NdeII GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 8 cut(s) 93, 164, 442, 483, 569, 1018, 1143, 1172
NlaIV GGNNCC 3 cut(s) 538, 941, 1224
NmuCI GTSAC 2 cut(s) 18, 412
NspI RCATGY 1 cut(s) 1143
PagI TCATGA 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PctI GAATGC 2 cut(s) 190, 561
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 236, 508, 875
PinAI ACCGGT 1 cut(s) 22
PkrI GCNGC 4 cut(s) 42, 256, 735, 1107
PleI GAGTC 1 cut(s) 1066
PpsI GAGTC 1 cut(s) 1066
PshBI ATTAAT 1 cut(s) 501
Psp6I CCWGG 2 cut(s) 53, 64
PspEI GGTNACC 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 1208
PspGI CCWGG 2 cut(s) 53, 64
PspN4I GGNNCC 3 cut(s) 538, 941, 1224
PspPI GGNCC 2 cut(s) 536, 940
PstI CTGCAG 3 cut(s) 45, 735, 804
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 2 cut(s) 74, 853
RsaI GTAC 4 cut(s) 146, 729, 1028, 1283
RsaNI GTAC 4 cut(s) 145, 728, 1027, 1282
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 8 cut(s) 291, 321, 501, 518, 861, 926, 1193, 1290
SatI GCNGC 4 cut(s) 41, 255, 734, 1106
Sau3AI GATC 8 cut(s) 74, 132, 379, 469, 763, 853, 1011, 1073
Sau96I GGNCC 2 cut(s) 536, 940
SchI GAGTC 1 cut(s) 1067
ScrFI CCNGG 3 cut(s) 55, 66, 303
SduI GDGCHC 1 cut(s) 1042
SfaNI GCATC 4 cut(s) 176, 186, 276, 797
SfcI CTRYAG 5 cut(s) 41, 651, 731, 800, 1062
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 3 cut(s) 395, 1178, 1199
SmoI CTYRAG 3 cut(s) 395, 1178, 1199
Sse9I AATT 4 cut(s) 322, 354, 552, 858
SsiI CCGC 1 cut(s) 890
SspI AATATT 1 cut(s) 193
SspMI CTAG 2 cut(s) 156, 665
StyD4I CCNGG 3 cut(s) 53, 64, 301
StyI CCWWGG 2 cut(s) 89, 438
TaaI ACNGT 1 cut(s) 1066
TaqI TCGA 5 cut(s) 180, 468, 681, 1070, 1343
TasI AATT 4 cut(s) 322, 354, 552, 858
TatI WGTACW 1 cut(s) 1026
TfiI GAWTC 3 cut(s) 236, 508, 875
Tru1I TTAA 8 cut(s) 291, 321, 501, 518, 861, 926, 1193, 1290
Tru9I TTAA 8 cut(s) 291, 321, 501, 518, 861, 926, 1193, 1290
TscAI CASTG 2 cut(s) 500, 904
TseFI GTSAC 2 cut(s) 18, 412
TseI GCWGC 4 cut(s) 40, 254, 733, 1105
Tsp45I GTSAC 2 cut(s) 18, 412
TspDTI ATGAA 7 cut(s) 21, 449, 464, 468, 554, 582, 833
TspGWI ACGGA 4 cut(s) 715, 1175, 1254, 1271
TspRI CASTG 2 cut(s) 500, 904
VspI ATTAAT 1 cut(s) 501
XagI CCTNNNNNAGG 1 cut(s) 69
XapI RAATTY 1 cut(s) 354
XceI RCATGY 1 cut(s) 1143
XcmI CCANNNNNNNNNTGG 1 cut(s) 87
XmiI GTMKAC 1 cut(s) 1061
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 2 cut(s) 156, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.