MD07G1030000.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002137
Physical Location & Seq
Forward (+)
2514355 .. 2517053
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1030000.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGCGCAAGAGGACGGGTCACCAGTAACTTCATCACCCCTGCAGTTCTTTCCCTGGCTATCTCCTGGGATGGGATCTCCATACTCACCATGGCTCCGGGAGCTAAAATCTGAGGAGAGAGGTTTATGTCTGATCCAGCTTCTTTACGCATGTGCTAGCCATGTCACTTCTGGTAGCATTGAGAAATCAAATTTTTGGCTTGACCAGATTTCTCAGCTCGCCTCTCCTGATGGCGATTCAATGCAGAGAATAGCTGCTTATTTCACTGAGGCACTTGCTGACCGGATGCTTAAAGGTTGGCCCGGTCTGCATAAAGCGCTTCATTCAACGAAAATATCATCAGTCTCAGAAGAAATTATTGTTCAAAGGCTGTTCTTTGATCTCTGTCCTTTCCTAAAGGTTGCGTATGTGGTCACAAATCAGGCCATAGTGGAAGCCATGGAAGGGGAGAAGATGGTTCATATTATCGATCTTCATTCATGTGAGCCCGCTCAGTGGATTAATTTGATTCAAACATTGAATGCGCGACCTGAAGGCCCTCCCCATTTGAGAATTACGGGCATTAATGAACAAAAAGAAGTATTAGACCAAATGTTCCTTCAGCTGACAGAAGAAGCCGGAAATCTGAACATCCCATTTCAGTTCAATGCTATAGTAAGCAAGTTAGAGAACCTTGATATCGAAAGTTTGCGTGTCAAGACTGGAGAGGCTCTTGCAATCTGCTCCGTTCTGCAGCTACATTCGTTCTTGGCAAGTGATGATGATCATAGGAGGAAATCACCATTAGCATCTAAGAACCTGCAGAAAGTCTTGCATATGAATAAACTCACTTTGGGAGAGTGGCTGGAGAAAGATCCAATCAGTGCATATAACCTGAGCCCCGATACTGCTTGGTCCCCGCTATCAGGTGCTTCACCAACGGGGAGTTTTTTAACTTCTCTTTGGGGCCTTTCGCCAAAACTAATGGTAATAACCGAGCAAGAGGCAAACCACAATGGCCATACATTGATGGACAGGATCATGGAAGCATTGTACTTCTATGGAGCACTTTTTGATTGCTTAGAGTCTACAGTATCAAGAGCACCAATGGAGCGACAAAAGGTTGAGAAGATGCTTTTCGGAGAGGAAATAAAAAACATCATAGCATGTGAAGGAACTGAGCGCAGGGAGAGGCATGAGAAGCTTGAGAAGTGGGTTCTAAGACTTGAGTTGGCAGGGTTTGGCAAGGTGCCTTTGAGCTACAATGGAATGTTGCAGGCTAGGGGGCTCTTGCGTGGCTATGAAGGGTATAAGATTAAAGAAGAGAATGGATGTGTGTTTATTTGCTGGCATGACAGACCTCTTTTTACGATTTCAGGCTGGAGGTCTAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

459

Amino Acids

51.77

Weight (kDa)

5.86

Isoelectric Point (pI)

53.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 44 - 455 2.8e-117 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 1228
AccBSI CCGCTC 1 cut(s) 491
AccI GTMKAC 1 cut(s) 1067
AccII CGCG 1 cut(s) 526
AciI CCGC 2 cut(s) 489, 899
AclWI GGATC 4 cut(s) 82, 127, 848, 1025
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 2 cut(s) 552, 584
AfaI GTAC 1 cut(s) 1034
AfeI AGCGCT 1 cut(s) 318
AfiI CCNNNNNNNGG 4 cut(s) 71, 444, 495, 905
AgsI TTSAA 6 cut(s) 240, 327, 365, 512, 520, 646
AjnI CCWGG 2 cut(s) 53, 64
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
AluBI AGCT 8 cut(s) 103, 139, 217, 254, 604, 736, 1183, 1239
AluI AGCT 8 cut(s) 103, 139, 217, 254, 604, 736, 1183, 1239
Alw21I GWGCWC 2 cut(s) 1048, 1084
Alw26I GTCTC 1 cut(s) 349
AlwI GGATC 4 cut(s) 82, 127, 848, 1025
Aor51HI AGCGCT 1 cut(s) 318
AoxI GGCC 5 cut(s) 299, 423, 535, 946, 997
ApeKI GCWGC 2 cut(s) 254, 733
ApoI RAATTY 1 cut(s) 190
AseI ATTAAT 2 cut(s) 501, 564
AspLEI GCGC 4 cut(s) 7, 319, 526, 1164
AspS9I GGNCC 4 cut(s) 300, 536, 894, 946
AsuC2I CCSGG 2 cut(s) 98, 303
AsuHPI GGTGA 5 cut(s) 12, 27, 78, 771, 906
AsuNHI GCTAGC 1 cut(s) 155
AvaII GGWCC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 999
BanI GGYRCC 1 cut(s) 1228
BanII GRGCYC 3 cut(s) 489, 881, 1269
Bbv12I GWGCWC 2 cut(s) 1048, 1084
BbvI GCAGC 2 cut(s) 241, 745
BccI CCATC 4 cut(s) 64, 224, 448, 1003
BciT130I CCWGG 2 cut(s) 55, 66
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 2 cut(s) 98, 303
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 3 cut(s) 156, 1260, 1368
BfmI CTRYAG 5 cut(s) 41, 651, 731, 800, 1068
BfoI RGCGCY 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 807
BisI GCNGC 2 cut(s) 255, 734
BlsI GCNGC 2 cut(s) 256, 735
Bme1390I CCNGG 4 cut(s) 55, 66, 98, 303
Bme18I GGWCC 1 cut(s) 894
BmgT120I GGNCC 4 cut(s) 300, 536, 894, 946
BmiI GGNNCC 4 cut(s) 95, 896, 947, 1230
BmrFI CCNGG 4 cut(s) 55, 66, 98, 303
BmsI GCATC 3 cut(s) 276, 797, 1101
BmtI GCTAGC 1 cut(s) 159
BpmI CTGGAG 2 cut(s) 723, 866
Bpu10I CCTNAGC 1 cut(s) 875
BpuEI CTTGAG 2 cut(s) 1205, 1226
BpuMI CCSGG 2 cut(s) 98, 303
Bsa29I ATCGAT 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 4 cut(s) 53, 65, 89, 438
BsaWI WCCGGW 1 cut(s) 282
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 444, 495, 905
Bse1I ACTGG 2 cut(s) 23, 706
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 2 cut(s) 55, 66
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 4 cut(s) 53, 65, 89, 438
BseGI GGATG 4 cut(s) 75, 291, 630, 1316
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 4 cut(s) 71, 444, 495, 905
BseMII CTCAG 7 cut(s) 102, 227, 258, 360, 506, 866, 1149
BseNI ACTGG 2 cut(s) 23, 706
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 241, 745
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 5 cut(s) 301, 425, 537, 948, 999
BshNI GGYRCC 1 cut(s) 1228
BshVI ATCGAT 1 cut(s) 468
BsiHKAI GWGCWC 2 cut(s) 1048, 1084
BsiSI CCGG 4 cut(s) 97, 283, 303, 618
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 4 cut(s) 71, 444, 495, 905
BsmAI GTCTC 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 880
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 5 cut(s) 301, 425, 537, 948, 999
Bsp1286I GDGCHC 5 cut(s) 489, 881, 1048, 1084, 1269
Bsp143I GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
Bsp19I CCATGG 2 cut(s) 89, 438
BspACI CCGC 2 cut(s) 489, 899
BspANI GGCC 5 cut(s) 301, 425, 537, 948, 999
BspCNI CTCAG 7 cut(s) 103, 226, 259, 359, 505, 867, 1150
BspDI ATCGAT 1 cut(s) 468
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 4 cut(s) 95, 896, 947, 1230
BspMAI CTGCAG 3 cut(s) 45, 735, 804
BspMI ACCTGC 1 cut(s) 807
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 4 cut(s) 82, 127, 848, 1025
BspT107I GGYRCC 1 cut(s) 1228
BsrBI CCGCTC 1 cut(s) 491
BsrI ACTGG 2 cut(s) 23, 706
BssECI CCNNGG 4 cut(s) 53, 65, 89, 438
BssMI GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
BssT1I CCWWGG 2 cut(s) 89, 438
Bst2UI CCWGG 2 cut(s) 55, 66
Bst4CI ACNGT 1 cut(s) 1072
Bst6I CTCTTC 1 cut(s) 1296
BstC8I GCNNGC 5 cut(s) 157, 219, 489, 1257, 1328
BstDSI CCRYGG 2 cut(s) 89, 438
BstEII GGTNACC 1 cut(s) 18
BstF5I GGATG 4 cut(s) 75, 291, 630, 1316
BstFNI CGCG 1 cut(s) 526
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 4 cut(s) 7, 319, 526, 1164
BstKTI GATC 7 cut(s) 77, 135, 382, 472, 766, 856, 1020
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
BstMWI GCNNNNNNNGC 4 cut(s) 100, 307, 316, 1180
BstNI CCWGG 2 cut(s) 55, 66
BstNSI RCATGY 2 cut(s) 153, 1149
BstPI GGTNACC 1 cut(s) 18
BstSCI CCNGG 4 cut(s) 53, 64, 96, 301
BstSFI CTRYAG 5 cut(s) 41, 651, 731, 800, 1068
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 2 cut(s) 241, 745
BstX2I RGATCY 2 cut(s) 74, 853
BstYI RGATCY 2 cut(s) 74, 853
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 5 cut(s) 301, 425, 537, 948, 999
BsuTUI ATCGAT 1 cut(s) 468
BtgI CCRYGG 2 cut(s) 89, 438
BtsCI GGATG 4 cut(s) 75, 291, 630, 1316
BtsIMutI CAGTG 3 cut(s) 264, 500, 868
BveI ACCTGC 1 cut(s) 807
Cac8I GCNNGC 5 cut(s) 157, 219, 489, 1257, 1328
CfoI GCGC 4 cut(s) 7, 319, 526, 1164
Cfr13I GGNCC 4 cut(s) 300, 536, 894, 946
ClaI ATCGAT 1 cut(s) 468
Csp6I GTAC 1 cut(s) 1033
CviAII CATG 9 cut(s) 90, 150, 161, 439, 480, 1021, 1146, 1175, 1331
CviQI GTAC 1 cut(s) 1033
DpnI GATC 7 cut(s) 76, 134, 381, 471, 765, 855, 1019
DpnII GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 1 cut(s) 1296
EarI CTCTTC 1 cut(s) 1296
Eco130I CCWWGG 2 cut(s) 89, 438
Eco24I GRGCYC 3 cut(s) 489, 881, 1269
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 894
Eco47III AGCGCT 1 cut(s) 318
Eco57I CTGAAG 2 cut(s) 552, 584
Eco91I GGTNACC 1 cut(s) 18
EcoO109I RGGNCCY 2 cut(s) 536, 946
EcoO65I GGTNACC 1 cut(s) 18
EcoRII CCWGG 2 cut(s) 53, 64
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 2 cut(s) 89, 438
EcoT38I GRGCYC 3 cut(s) 489, 881, 1269
ErhI CCWWGG 2 cut(s) 89, 438
FaeI CATG 9 cut(s) 93, 153, 164, 442, 483, 1024, 1149, 1178, 1334
FaqI GGGAC 1 cut(s) 880
FatI CATG 9 cut(s) 89, 149, 160, 438, 479, 1020, 1145, 1174, 1330
FauI CCCGC 2 cut(s) 496, 906
FauNDI CATATG 1 cut(s) 816
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 1067
Fnu4HI GCNGC 2 cut(s) 255, 734
FokI GGATG 4 cut(s) 82, 298, 617, 1323
FriOI GRGCYC 3 cut(s) 489, 881, 1269
Fsp4HI GCNGC 2 cut(s) 255, 734
FspBI CTAG 3 cut(s) 156, 1260, 1368
GlaI GCGC 4 cut(s) 6, 318, 525, 1163
GluI GCNGC 2 cut(s) 255, 734
GsuI CTGGAG 2 cut(s) 723, 866
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 5 cut(s) 301, 425, 537, 948, 999
HapII CCGG 4 cut(s) 97, 283, 303, 618
HhaI GCGC 4 cut(s) 7, 319, 526, 1164
Hin1II CATG 9 cut(s) 93, 153, 164, 442, 483, 1024, 1149, 1178, 1334
Hin6I GCGC 4 cut(s) 5, 317, 524, 1162
HinP1I GCGC 4 cut(s) 5, 317, 524, 1162
HindIII AAGCTT 1 cut(s) 1181
HinfI GANTC 3 cut(s) 236, 508, 1064
HpaII CCGG 4 cut(s) 97, 283, 303, 618
HphI GGTGA 5 cut(s) 12, 27, 78, 771, 906
Hpy166II GTNNAC 1 cut(s) 1068
Hpy188I TCNGA 5 cut(s) 112, 132, 349, 627, 1121
Hpy188III TCNNGA 3 cut(s) 227, 697, 1077
Hpy8I GTNNAC 1 cut(s) 1068
HpyAV CCTTC 5 cut(s) 437, 527, 608, 1145, 1277
HpyCH4III ACNGT 1 cut(s) 1072
HpyCH4V TGCA 9 cut(s) 43, 244, 310, 716, 733, 802, 814, 866, 1255
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 307, 316, 1180
Hsp92II CATG 9 cut(s) 93, 153, 164, 442, 483, 1024, 1149, 1178, 1334
HspAI GCGC 4 cut(s) 5, 317, 524, 1162
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
LmnI GCTCC 5 cut(s) 99, 100, 728, 1043, 1090
Lsp1109I GCAGC 2 cut(s) 241, 745
LweI GCATC 3 cut(s) 276, 797, 1101
MaeI CTAG 3 cut(s) 156, 1260, 1368
MaeIII GTNAC 4 cut(s) 18, 25, 163, 412
MalI GATC 7 cut(s) 76, 134, 381, 471, 765, 855, 1019
MbiI CCGCTC 1 cut(s) 491
MboI GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
MboII GAAGA 6 cut(s) 362, 463, 464, 623, 1120, 1313
MflI RGATCY 2 cut(s) 74, 853
MhlI GDGCHC 5 cut(s) 489, 881, 1048, 1084, 1269
MlsI TGGCCA 1 cut(s) 999
MluCI AATT 4 cut(s) 190, 354, 502, 552
MluNI TGGCCA 1 cut(s) 999
MlyI GAGTC 1 cut(s) 1073
Mox20I TGGCCA 1 cut(s) 999
MscI TGGCCA 1 cut(s) 999
MseI TTAA 5 cut(s) 291, 501, 564, 932, 1296
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 999
MspA1I CMGCKG 1 cut(s) 604
MspI CCGG 4 cut(s) 97, 283, 303, 618
MspR9I CCNGG 4 cut(s) 55, 66, 98, 303
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 2 cut(s) 55, 66
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 4 cut(s) 100, 307, 316, 1180
NciI CCSGG 2 cut(s) 98, 303
NcoI CCATGG 2 cut(s) 89, 438
NdeI CATATG 1 cut(s) 816
NdeII GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 9 cut(s) 93, 153, 164, 442, 483, 1024, 1149, 1178, 1334
NlaIV GGNNCC 4 cut(s) 95, 896, 947, 1230
NmuCI GTSAC 3 cut(s) 18, 163, 412
NspI RCATGY 2 cut(s) 153, 1149
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 236, 508
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 2 cut(s) 256, 735
PleI GAGTC 1 cut(s) 1072
PpsI GAGTC 1 cut(s) 1072
PshBI ATTAAT 2 cut(s) 501, 564
Psp6I CCWGG 2 cut(s) 53, 64
PspEI GGTNACC 1 cut(s) 18
PspGI CCWGG 2 cut(s) 53, 64
PspN4I GGNNCC 4 cut(s) 95, 896, 947, 1230
PspPI GGNCC 4 cut(s) 300, 536, 894, 946
PstI CTGCAG 3 cut(s) 45, 735, 804
PsuI RGATCY 2 cut(s) 74, 853
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 1 cut(s) 1034
RsaNI GTAC 1 cut(s) 1033
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 5 cut(s) 291, 501, 564, 932, 1296
SatI GCNGC 2 cut(s) 255, 734
Sau3AI GATC 7 cut(s) 74, 132, 379, 469, 763, 853, 1017
Sau96I GGNCC 4 cut(s) 300, 536, 894, 946
SchI GAGTC 1 cut(s) 1073
ScrFI CCNGG 4 cut(s) 55, 66, 98, 303
SduI GDGCHC 5 cut(s) 489, 881, 1048, 1084, 1269
SfaNI GCATC 3 cut(s) 276, 797, 1101
SfcI CTRYAG 5 cut(s) 41, 651, 731, 800, 1068
SinI GGWCC 1 cut(s) 894
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 2 cut(s) 1184, 1205
SmoI CTYRAG 2 cut(s) 1184, 1205
Sse9I AATT 4 cut(s) 190, 354, 502, 552
SsiI CCGC 2 cut(s) 489, 899
SspMI CTAG 3 cut(s) 156, 1260, 1368
StyD4I CCNGG 4 cut(s) 53, 64, 96, 301
StyI CCWWGG 2 cut(s) 89, 438
TaaI ACNGT 1 cut(s) 1072
TaqI TCGA 2 cut(s) 468, 681
TasI AATT 4 cut(s) 190, 354, 502, 552
TatI WGTACW 1 cut(s) 1032
TfiI GAWTC 2 cut(s) 236, 508
Tru1I TTAA 5 cut(s) 291, 501, 564, 932, 1296
Tru9I TTAA 5 cut(s) 291, 501, 564, 932, 1296
TscAI CASTG 3 cut(s) 271, 500, 868
TseFI GTSAC 3 cut(s) 18, 163, 412
TseI GCWGC 2 cut(s) 254, 733
Tsp45I GTSAC 3 cut(s) 18, 163, 412
TspDTI ATGAA 8 cut(s) 21, 311, 449, 464, 468, 582, 833, 1296
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 3 cut(s) 271, 500, 868
VpaK11BI GGWCC 1 cut(s) 894
VspI ATTAAT 2 cut(s) 501, 564
XapI RAATTY 1 cut(s) 190
XceI RCATGY 2 cut(s) 153, 1149
XcmI CCANNNNNNNNNTGG 2 cut(s) 87, 167
XmiI GTMKAC 1 cut(s) 1067
XspI CTAG 3 cut(s) 156, 1260, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.