pycom07g02200

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002137
Physical Location & Seq
Forward (+)
1790237 .. 1791175
939 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02200.1

Sequence Viewer

Length: 939 bp
ATGGAAGGGGAGAAGATGGTTCATATGATCGATCTTCATTCATGTGAGCCCGCTCAGTGGATTAATTTGATTCAAACACTGAATGCGCGACCTGAAGGCCCTCCCCATTTGAGAATTACGGGCATTAATGAACAAAAAGAAGTATTAGACCAAATGTTCCTTCAGTTGACAGAAGAAGCCGGAAATCTGAACATCCCATTTCAGTTCAATGCTATAGTAAGCAAGTTAGAGAACCTTGACATTGAAAGTTTGCGTGTCAAGACTGGAGAGGCTCTTGCAATCTGCTCCGTTCTGCAGCTACATTCGTTCTTGGCAAGTGATGATGATCAAAGGAGGAAATCACCATTAGCATCTAAGAACCTGCAGAAAGTCTTGCATATGAATAAACTCACTTTGGGAGAATGGCTGGAGAAAGATCCAATCAGTGCATATAACCTGAGTCCCGACACTGCTTGGTCCCCGCTATCAGGTGCTTCACCAACGGGGAGTTTTTTAACTTCTCTTTGGGGCCTTTCGCCAAAACTAATGGTAATAACCGAGCAAGAGGCAAACCACAATGGCCATACATTGATGGTCACGATCATGGAAGCATTGCACTTTTATGGAGCACTTTTTGATTGCTTAGAGTCTACAGTATCAAGAGCACCGATGGAGCGACAAAAGGTTGAGAAGATGCTTTTCGGAGAGGAAATAAAAAACATCATAGCATGTGAAGGAACTGAGCGCAGGGAGAGGCATGAGAAGCTTGAGAAGTGGATTCTAAGACTTGAGTTGGCAGGGTTTGGCAAGGTGCCCTTGAGCTACAATGGAATGTTGCAGGCTAGGGGGTTCTTGCATGGCTATGACGGGTATAAGATTAAAGAAGAGAATGGATGTGTGTTTATTTGCTGGCATGACAGACCTCTTTTTACGATTTCAGGCTGGAGATCTAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

313

Amino Acids

35.49

Weight (kDa)

6.02

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 1 - 309 1.1e-81 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 53
AccI GTMKAC 1 cut(s) 629
AccII CGCG 1 cut(s) 88
AciI CCGC 2 cut(s) 51, 461
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 559
AcuI CTGAAG 2 cut(s) 114, 146
AfiI CCNNNNNNNGG 2 cut(s) 57, 467
AgsI TTSAA 3 cut(s) 74, 208, 245
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AluBI AGCT 3 cut(s) 298, 745, 801
AluI AGCT 3 cut(s) 298, 745, 801
Alw21I GWGCWC 2 cut(s) 610, 646
AlwI GGATC 1 cut(s) 410
AoxI GGCC 3 cut(s) 97, 508, 559
ApeKI GCWGC 1 cut(s) 295
AseI ATTAAT 2 cut(s) 63, 126
AspLEI GCGC 2 cut(s) 88, 726
AspS9I GGNCC 3 cut(s) 98, 456, 508
AsuHPI GGTGA 2 cut(s) 333, 468
AvaII GGWCC 1 cut(s) 456
BaeGI GKGCMC 1 cut(s) 795
BalI TGGCCA 1 cut(s) 561
BanI GGYRCC 1 cut(s) 790
BanII GRGCYC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 610, 646
BbvI GCAGC 1 cut(s) 307
BccI CCATC 3 cut(s) 10, 565, 643
BclI TGATCA 1 cut(s) 325
BfaI CTAG 2 cut(s) 822, 930
BfmI CTRYAG 4 cut(s) 213, 293, 362, 630
BfuAI ACCTGC 1 cut(s) 369
BglII AGATCT 1 cut(s) 926
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 98, 456, 508
BmiI GGNNCC 3 cut(s) 458, 509, 792
BmsI GCATC 2 cut(s) 359, 663
BpmI CTGGAG 2 cut(s) 285, 428
BpuEI CTTGAG 3 cut(s) 767, 788, 817
Bsa29I ATCGAT 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 324
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 467
Bse1I ACTGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 590
Bse8I GATNNNNATC 1 cut(s) 324
BseCI ATCGAT 1 cut(s) 30
BseGI GGATG 2 cut(s) 192, 878
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 2 cut(s) 57, 467
BseMI GCAATG 1 cut(s) 590
BseMII CTCAG 3 cut(s) 68, 428, 711
BseNI ACTGG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 795
BseXI GCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 3 cut(s) 99, 510, 561
BshNI GGYRCC 1 cut(s) 790
BshVI ATCGAT 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 610, 646
BsiSI CCGG 1 cut(s) 180
BslFI GGGAC 2 cut(s) 426, 442
BslI CCNNNNNNNGG 2 cut(s) 57, 467
BsmFI GGGAC 2 cut(s) 426, 442
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 3 cut(s) 99, 510, 561
Bsp1286I GDGCHC 4 cut(s) 51, 610, 646, 795
Bsp143I GATC 6 cut(s) 27, 31, 325, 415, 579, 926
BspACI CCGC 2 cut(s) 51, 461
BspANI GGCC 3 cut(s) 99, 510, 561
BspCNI CTCAG 3 cut(s) 67, 429, 712
BspDI ATCGAT 1 cut(s) 30
BspFNI CGCG 1 cut(s) 88
BspLI GGNNCC 3 cut(s) 458, 509, 792
BspMAI CTGCAG 2 cut(s) 297, 366
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 790
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 590
BsrI ACTGG 1 cut(s) 268
BssMI GATC 6 cut(s) 27, 31, 325, 415, 579, 926
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 1 cut(s) 858
BstC8I GCNNGC 3 cut(s) 51, 819, 890
BstDEI CTNAG 6 cut(s) 54, 354, 437, 622, 720, 761
BstF5I GGATG 2 cut(s) 192, 878
BstFNI CGCG 1 cut(s) 88
BstHHI GCGC 2 cut(s) 88, 726
BstKTI GATC 6 cut(s) 30, 34, 328, 418, 582, 929
BstMBI GATC 6 cut(s) 27, 31, 325, 415, 579, 926
BstMWI GCNNNNNNNGC 1 cut(s) 742
BstNSI RCATGY 1 cut(s) 711
BstSFI CTRYAG 4 cut(s) 213, 293, 362, 630
BstSLI GKGCMC 1 cut(s) 795
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 307
BstX2I RGATCY 2 cut(s) 415, 926
BstYI RGATCY 2 cut(s) 415, 926
Bsu15I ATCGAT 1 cut(s) 30
BsuRI GGCC 3 cut(s) 99, 510, 561
BsuTUI ATCGAT 1 cut(s) 30
BtsCI GGATG 2 cut(s) 192, 878
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 4 cut(s) 62, 77, 430, 447
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 51, 819, 890
CfoI GCGC 2 cut(s) 88, 726
Cfr13I GGNCC 3 cut(s) 98, 456, 508
ClaI ATCGAT 1 cut(s) 30
CviAII CATG 6 cut(s) 42, 583, 708, 737, 836, 893
DdeI CTNAG 6 cut(s) 54, 354, 437, 622, 720, 761
DpnI GATC 6 cut(s) 29, 33, 327, 417, 581, 928
DpnII GATC 6 cut(s) 27, 31, 325, 415, 579, 926
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 114, 146
EcoO109I RGGNCCY 2 cut(s) 98, 508
EcoT38I GRGCYC 1 cut(s) 51
FaeI CATG 6 cut(s) 45, 586, 711, 740, 839, 896
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FaqI GGGAC 2 cut(s) 426, 442
FatI CATG 6 cut(s) 41, 582, 707, 736, 835, 892
FauI CCCGC 2 cut(s) 58, 468
FauNDI CATATG 2 cut(s) 24, 378
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 629
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 2 cut(s) 179, 885
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 2 cut(s) 822, 930
GlaI GCGC 2 cut(s) 87, 725
GluI GCNGC 1 cut(s) 296
GsuI CTGGAG 2 cut(s) 285, 428
HaeIII GGCC 3 cut(s) 99, 510, 561
HapII CCGG 1 cut(s) 180
HhaI GCGC 2 cut(s) 88, 726
Hin1II CATG 6 cut(s) 45, 586, 711, 740, 839, 896
Hin6I GCGC 2 cut(s) 86, 724
HinP1I GCGC 2 cut(s) 86, 724
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 4 cut(s) 70, 439, 626, 757
HpaII CCGG 1 cut(s) 180
HphI GGTGA 2 cut(s) 333, 468
Hpy166II GTNNAC 2 cut(s) 168, 630
Hpy188I TCNGA 2 cut(s) 189, 683
Hpy188III TCNNGA 4 cut(s) 259, 443, 577, 639
Hpy8I GTNNAC 2 cut(s) 168, 630
HpyAV CCTTC 3 cut(s) 89, 170, 707
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4V TGCA 8 cut(s) 278, 295, 364, 376, 428, 595, 817, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 742
HpyF3I CTNAG 6 cut(s) 54, 354, 437, 622, 720, 761
Hsp92II CATG 6 cut(s) 45, 586, 711, 740, 839, 896
HspAI GCGC 2 cut(s) 86, 724
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 6 cut(s) 27, 31, 325, 415, 579, 926
LmnI GCTCC 3 cut(s) 290, 605, 652
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 2 cut(s) 359, 663
MaeI CTAG 2 cut(s) 822, 930
MaeIII GTNAC 1 cut(s) 574
MalI GATC 6 cut(s) 29, 33, 327, 417, 581, 928
MbiI CCGCTC 1 cut(s) 53
MboI GATC 6 cut(s) 27, 31, 325, 415, 579, 926
MboII GAAGA 5 cut(s) 25, 26, 185, 682, 875
MflI RGATCY 2 cut(s) 415, 926
MhlI GDGCHC 4 cut(s) 51, 610, 646, 795
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 2 cut(s) 64, 114
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 2 cut(s) 448, 635
MnlI CCTC 7 cut(s) 111, 262, 327, 538, 679, 726, 912
Mox20I TGGCCA 1 cut(s) 561
MscI TGGCCA 1 cut(s) 561
MseI TTAA 4 cut(s) 63, 126, 494, 858
MslI CAYNNNNRTG 4 cut(s) 42, 581, 600, 840
Msp20I TGGCCA 1 cut(s) 561
MspI CCGG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 88
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 742
NdeI CATATG 2 cut(s) 24, 378
NdeII GATC 6 cut(s) 27, 31, 325, 415, 579, 926
NlaIII CATG 6 cut(s) 45, 586, 711, 740, 839, 896
NlaIV GGNNCC 3 cut(s) 458, 509, 792
NmuCI GTSAC 1 cut(s) 574
NspI RCATGY 1 cut(s) 711
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 2 cut(s) 70, 757
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 2 cut(s) 447, 634
PpsI GAGTC 2 cut(s) 447, 634
PshBI ATTAAT 2 cut(s) 63, 126
PspN4I GGNNCC 3 cut(s) 458, 509, 792
PspPI GGNCC 3 cut(s) 98, 456, 508
PstI CTGCAG 2 cut(s) 297, 366
PsuI RGATCY 2 cut(s) 415, 926
RseI CAYNNNNRTG 4 cut(s) 42, 581, 600, 840
SaqAI TTAA 4 cut(s) 63, 126, 494, 858
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 6 cut(s) 27, 31, 325, 415, 579, 926
Sau96I GGNCC 3 cut(s) 98, 456, 508
SchI GAGTC 2 cut(s) 448, 635
SduI GDGCHC 4 cut(s) 51, 610, 646, 795
SfaNI GCATC 2 cut(s) 359, 663
SfcI CTRYAG 4 cut(s) 213, 293, 362, 630
SinI GGWCC 1 cut(s) 456
SmiMI CAYNNNNRTG 4 cut(s) 42, 581, 600, 840
SmlI CTYRAG 3 cut(s) 746, 767, 796
SmoI CTYRAG 3 cut(s) 746, 767, 796
Sse9I AATT 2 cut(s) 64, 114
SsiI CCGC 2 cut(s) 51, 461
SspMI CTAG 2 cut(s) 822, 930
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 1 cut(s) 30
TasI AATT 2 cut(s) 64, 114
TfiI GAWTC 2 cut(s) 70, 757
Tru1I TTAA 4 cut(s) 63, 126, 494, 858
Tru9I TTAA 4 cut(s) 63, 126, 494, 858
TscAI CASTG 4 cut(s) 62, 84, 430, 454
TseFI GTSAC 1 cut(s) 574
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 574
TspDTI ATGAA 5 cut(s) 11, 26, 30, 144, 395
TspGWI ACGGA 1 cut(s) 277
TspRI CASTG 4 cut(s) 62, 84, 430, 454
VpaK11BI GGWCC 1 cut(s) 456
VspI ATTAAT 2 cut(s) 63, 126
XceI RCATGY 1 cut(s) 711
XmiI GTMKAC 1 cut(s) 629
XspI CTAG 2 cut(s) 822, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.