FvH4_7g08390

Cupin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004244
Physical Location & Seq
Forward (+)
8291087 .. 8292114
1028 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08390.t1

Sequence Viewer

Length: 708 bp
ATGTTAAACACAGAGCCATATACAAGAGAAACAGCTAGAAGTACCATATATCTTGCCCTAGCTCGTTATTCATCAGCCATGTCTGCAACTATGAGATTGATTCAGTTACTGGTGATCATATCAGTAGTAGCTTTGGCCATCTCAAGAAGAGCGGTTGCAAGTGATCCTGACATCTCTTCCGATTTCATACTACCAGAAAACCACACCGGCACAACTGTTGATGCCTCATTCTTCACATACACAGGGTTCCGCGGCTTATTTGATCAAACCCCCGAAATCTTCACGGCAACAAAAGCAAGCATGGACGAGTTCCCTGCTCTGAAAGGCCAAAGTGTGTCATACGCCATGCTTCAGTTCCCTCCCAACACAATATTCCCACCTCACACCCGCCTGGACTCTGCCGGACTTATGTTAGTCCTTACTGGTTCTTTGGAAGTTGGACTAATCGACTCGAAAAACGTTTTGTATATTAAAAAGCTTCAAGCTGGAGATATGTTTGTGTTTCCTAAGGGATTGATCCATTATCAGTATAATGCGGATCCTAATTTGCCAGCCACTGCTATTGCATCATTTGGAAGCGCAAGCGCTAGATCAGTTGCAGTTCCAGACTCTATCTTCACCTCTGGAATTCGTGAAGCCGTCTTGGCAAAATCATTCAAGACAGATATTAGTACTGTTAGGAAGATCAGGGCTGGCCTAAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

236

Amino Acids

25.54

Weight (kDa)

9.1

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 94 - 226 9.8e-25 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 1 cut(s) 252
AciI CCGC 5 cut(s) 152, 250, 252, 388, 536
AclI AACGTT 1 cut(s) 459
AclWI GGATC 4 cut(s) 158, 511, 533, 546
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 335
AfaI GTAC 2 cut(s) 43, 673
AfeI AGCGCT 1 cut(s) 586
AgsI TTSAA 2 cut(s) 482, 658
AjnI CCWGG 1 cut(s) 390
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
AluBI AGCT 5 cut(s) 35, 62, 131, 478, 485
AluI AGCT 5 cut(s) 35, 62, 131, 478, 485
AlwI GGATC 4 cut(s) 158, 511, 533, 546
AlwNI CAGNNNCTG 2 cut(s) 109, 557
Aor51HI AGCGCT 1 cut(s) 586
AoxI GGCC 3 cut(s) 135, 325, 694
ApoI RAATTY 1 cut(s) 627
AspLEI GCGC 2 cut(s) 581, 587
AsuHPI GGTGA 2 cut(s) 124, 610
AxyI CCTNAGG 1 cut(s) 507
BalI TGGCCA 1 cut(s) 137
BamHI GGATCC 1 cut(s) 538
BccI CCATC 1 cut(s) 146
BceAI ACGGC 2 cut(s) 300, 623
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 1 cut(s) 392
BclI TGATCA 2 cut(s) 114, 262
BfaI CTAG 3 cut(s) 36, 59, 588
BfoI RGCGCY 1 cut(s) 588
BglI GCCNNNNNGGC 1 cut(s) 644
BisI GCNGC 1 cut(s) 253
BlsI GCNGC 1 cut(s) 254
BmcAI AGTACT 1 cut(s) 673
Bme1390I CCNGG 1 cut(s) 392
BmiI GGNNCC 2 cut(s) 248, 540
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 2 cut(s) 211, 575
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 2 cut(s) 114, 427
Bse21I CCTNAGG 1 cut(s) 507
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 114, 427
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 3 cut(s) 137, 327, 696
BsiSI CCGG 2 cut(s) 207, 402
BsnI GGCC 3 cut(s) 137, 327, 696
Bsp143I GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
BspACI CCGC 5 cut(s) 152, 250, 252, 388, 536
BspANI GGCC 3 cut(s) 137, 327, 696
BspFNI CGCG 1 cut(s) 252
BspLI GGNNCC 2 cut(s) 248, 540
BspPI GGATC 4 cut(s) 158, 511, 533, 546
BspQI GCTCTTC 1 cut(s) 142
BsrBI CCGCTC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 2 cut(s) 114, 427
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 2 cut(s) 217, 676
Bst6I CTCTTC 2 cut(s) 142, 181
BstC8I GCNNGC 4 cut(s) 298, 552, 583, 694
BstDEI CTNAG 1 cut(s) 507
BstDSI CCRYGG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 2 cut(s) 581, 587
BstKTI GATC 7 cut(s) 117, 166, 265, 519, 541, 593, 687
BstMBI GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
BstMWI GCNNNNNNNGC 3 cut(s) 83, 293, 644
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 390
BstUI CGCG 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
Bsu36I CCTNAGG 1 cut(s) 507
BsuRI GGCC 3 cut(s) 137, 327, 696
BtgI CCRYGG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 4 cut(s) 298, 552, 583, 694
CaiI CAGNNNCTG 2 cut(s) 109, 557
CfoI GCGC 2 cut(s) 581, 587
Cfr10I RCCGGY 1 cut(s) 206
Cfr42I CCGCGG 1 cut(s) 253
Csp6I GTAC 2 cut(s) 42, 672
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
CviAII CATG 3 cut(s) 79, 301, 346
CviQI GTAC 2 cut(s) 42, 672
DdeI CTNAG 1 cut(s) 507
DpnI GATC 7 cut(s) 116, 165, 264, 518, 540, 592, 686
DpnII GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 2 cut(s) 142, 181
EarI CTCTTC 2 cut(s) 142, 181
Eco47III AGCGCT 1 cut(s) 586
Eco57I CTGAAG 1 cut(s) 335
Eco81I CCTNAGG 1 cut(s) 507
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 1 cut(s) 390
FaeI CATG 3 cut(s) 82, 304, 349
FatI CATG 3 cut(s) 78, 300, 345
FauI CCCGC 1 cut(s) 395
FbaI TGATCA 2 cut(s) 114, 262
Fnu4HI GCNGC 1 cut(s) 253
Fsp4HI GCNGC 1 cut(s) 253
FspBI CTAG 3 cut(s) 36, 59, 588
GlaI GCGC 2 cut(s) 580, 586
GluI GCNGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 3 cut(s) 137, 327, 696
HapII CCGG 2 cut(s) 207, 402
HhaI GCGC 2 cut(s) 581, 587
Hin1II CATG 3 cut(s) 82, 304, 349
Hin6I GCGC 2 cut(s) 579, 585
HinP1I GCGC 2 cut(s) 579, 585
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 4 cut(s) 100, 395, 449, 608
HpaII CCGG 2 cut(s) 207, 402
HphI GGTGA 2 cut(s) 124, 610
Hpy188I TCNGA 2 cut(s) 181, 321
Hpy188III TCNNGA 6 cut(s) 144, 167, 605, 624, 632, 658
HpyCH4III ACNGT 2 cut(s) 217, 676
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 4 cut(s) 86, 158, 566, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 293, 644
HpyF3I CTNAG 1 cut(s) 507
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 3 cut(s) 82, 304, 349
HspAI GCGC 2 cut(s) 579, 585
Ksp22I TGATCA 2 cut(s) 114, 262
KspI CCGCGG 1 cut(s) 253
Kzo9I GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
LguI GCTCTTC 1 cut(s) 142
LweI GCATC 2 cut(s) 211, 575
MaeI CTAG 3 cut(s) 36, 59, 588
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 105
MalI GATC 7 cut(s) 116, 165, 264, 518, 540, 592, 686
MbiI CCGCTC 1 cut(s) 152
MboI GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
MboII GAAGA 6 cut(s) 159, 168, 223, 271, 607, 694
MflI RGATCY 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 3 cut(s) 544, 627, 703
MluNI TGGCCA 1 cut(s) 137
MlyI GAGTC 3 cut(s) 389, 443, 602
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 4 cut(s) 235, 369, 390, 631
Mox20I TGGCCA 1 cut(s) 137
MscI TGGCCA 1 cut(s) 137
MseI TTAA 3 cut(s) 5, 471, 706
Msp20I TGGCCA 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 252
MspI CCGG 2 cut(s) 207, 402
MspR9I CCNGG 1 cut(s) 392
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 83, 293, 644
NdeII GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
NlaIII CATG 3 cut(s) 82, 304, 349
NlaIV GGNNCC 2 cut(s) 248, 540
PciSI GCTCTTC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 1 cut(s) 254
PleI GAGTC 3 cut(s) 389, 443, 602
PpsI GAGTC 3 cut(s) 389, 443, 602
Psp1406I AACGTT 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 248, 540
PstNI CAGNNNCTG 2 cut(s) 109, 557
PsuI RGATCY 1 cut(s) 538
RsaI GTAC 2 cut(s) 43, 673
RsaNI GTAC 2 cut(s) 42, 672
SacII CCGCGG 1 cut(s) 253
SapI GCTCTTC 1 cut(s) 142
SaqAI TTAA 3 cut(s) 5, 471, 706
SatI GCNGC 1 cut(s) 253
Sau3AI GATC 7 cut(s) 114, 163, 262, 516, 538, 590, 684
ScaI AGTACT 1 cut(s) 673
SchI GAGTC 3 cut(s) 389, 443, 602
ScrFI CCNGG 1 cut(s) 392
SetI ASST 8 cut(s) 37, 64, 133, 382, 462, 480, 487, 623
SfaNI GCATC 2 cut(s) 211, 575
Sfr303I CCGCGG 1 cut(s) 253
SgrBI CCGCGG 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 3 cut(s) 544, 627, 703
SsiI CCGC 5 cut(s) 152, 250, 252, 388, 536
SspI AATATT 1 cut(s) 372
SspMI CTAG 3 cut(s) 36, 59, 588
StyD4I CCNGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 217, 676
TaiI ACGT 1 cut(s) 462
TaqI TCGA 2 cut(s) 447, 452
TasI AATT 3 cut(s) 544, 627, 703
TatI WGTACW 1 cut(s) 671
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 3 cut(s) 5, 471, 706
Tru9I TTAA 3 cut(s) 5, 471, 706
TscAI CASTG 1 cut(s) 562
TspDTI ATGAA 2 cut(s) 60, 175
TspRI CASTG 1 cut(s) 562
XapI RAATTY 1 cut(s) 627
XspI CTAG 3 cut(s) 36, 59, 588
ZrmI AGTACT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.