Rroxscaffold_4G00311090

Cupin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004244
Physical Location & Seq
Reverse (-)
33352858 .. 33353670
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311090.1

Sequence Viewer

Length: 615 bp
ATGGCTATGACTATGAGACTGATCCAATTAATGGTGATCATATCGGTAATTGCTTTGGCCATCTCCAGAAGAGCGCTGGCAAGTGATCCTGACATCACTTCCGATTACATACTCCCAGAAAACCACACCAGCACAACTGTTGATGCCTCATTCTTCACATACACAGGATTCCGCGGCTTATTTGATCAAGCACCCAAAACCTTCACGGCAACAAAAGCAAGCATGGATGAGTTCCCTGCTCTCAAAGGCCAGAGTGTGTCATACACCATGCTTCAATTCCTCCGAACACATTATTCCCACCTCACACCCGCCTTGACTCGGCCGGACTCATGCCTAATTGACTCGAAAAACATTCTATATACTAAAAAGCTTCAAGCTGGAGATATGTTTGTGTTTCCTAAGGGATTGATCCATTATCAGTACAACGCGGATCCTAATTTGCCAGCCACTGCCATTGCAGCATTTGGAAGCGCAAGCGCTAGAACAGTTGCAGTTCCAGCCTCTATCTTCAGCTCTGGAATTCGCGATGTTGTCCTGGCAAAATCATTTAAGACAGATAATCATATATACTCTTCATCCATATCCAAGAATGAGAAGACAAACATAACTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

204

Amino Acids

22.45

Weight (kDa)

9.32

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 115 - 187 3.7e-12 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 31
AccII CGCG 3 cut(s) 174, 428, 525
AciI CCGC 4 cut(s) 172, 174, 309, 428
AclWI GGATC 5 cut(s) 16, 80, 403, 425, 438
AcoI YGGCCR 2 cut(s) 57, 320
AcsI RAATTY 1 cut(s) 519
AcuI CTGAAG 1 cut(s) 493
AfaI GTAC 1 cut(s) 422
AfeI AGCGCT 2 cut(s) 75, 478
AfiI CCNNNNNNNGG 2 cut(s) 31, 318
AgsI TTSAA 2 cut(s) 275, 374
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 3 cut(s) 370, 377, 513
AluI AGCT 3 cut(s) 370, 377, 513
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 5 cut(s) 16, 80, 403, 425, 438
AlwNI CAGNNNCTG 1 cut(s) 449
Aor51HI AGCGCT 2 cut(s) 75, 478
AoxI GGCC 3 cut(s) 57, 247, 320
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 1 cut(s) 519
AseI ATTAAT 1 cut(s) 29
AspLEI GCGC 3 cut(s) 76, 473, 479
AsuHPI GGTGA 1 cut(s) 46
AxyI CCTNAGG 1 cut(s) 399
BalI TGGCCA 1 cut(s) 59
BamHI GGATCC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 602
BbvI GCAGC 1 cut(s) 470
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 222
BciT130I CCWGG 1 cut(s) 536
BclI TGATCA 2 cut(s) 36, 184
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 1 cut(s) 480
BfoI RGCGCY 2 cut(s) 77, 480
BisI GCNGC 2 cut(s) 175, 459
BlsI GCNGC 2 cut(s) 176, 460
Bme1390I CCNGG 1 cut(s) 536
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 602
BpmI CTGGAG 2 cut(s) 49, 399
BsaJI CCNNGG 1 cut(s) 172
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 318
Bse21I CCTNAGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 2 cut(s) 232, 575
BseLI CCNNNNNNNGG 2 cut(s) 31, 318
BseMI GCAATG 1 cut(s) 453
BseX3I CGGCCG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 470
Bsh1236I CGCG 3 cut(s) 174, 428, 525
Bsh1285I CGRYCG 1 cut(s) 323
BshFI GGCC 3 cut(s) 59, 249, 322
BsiEI CGRYCG 1 cut(s) 323
BsiSI CCGG 1 cut(s) 323
BslI CCNNNNNNNGG 2 cut(s) 31, 318
BsmAI GTCTC 1 cut(s) 10
BsnI GGCC 3 cut(s) 59, 249, 322
Bsp143I GATC 6 cut(s) 21, 36, 85, 184, 408, 430
Bsp68I TCGCGA 1 cut(s) 525
BspACI CCGC 4 cut(s) 172, 174, 309, 428
BspANI GGCC 3 cut(s) 59, 249, 322
BspFNI CGCG 3 cut(s) 174, 428, 525
BspLI GGNNCC 1 cut(s) 432
BspPI GGATC 5 cut(s) 16, 80, 403, 425, 438
BspQI GCTCTTC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 453
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 6 cut(s) 21, 36, 85, 184, 408, 430
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 2 cut(s) 139, 487
Bst6I CTCTTC 2 cut(s) 64, 577
BstC8I GCNNGC 4 cut(s) 78, 220, 444, 475
BstDEI CTNAG 1 cut(s) 399
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 2 cut(s) 232, 575
BstFNI CGCG 3 cut(s) 174, 428, 525
BstH2I RGCGCY 2 cut(s) 77, 480
BstHHI GCGC 3 cut(s) 76, 473, 479
BstKTI GATC 6 cut(s) 24, 39, 88, 187, 411, 433
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 6 cut(s) 21, 36, 85, 184, 408, 430
BstMCI CGRYCG 1 cut(s) 323
BstMWI GCNNNNNNNGC 3 cut(s) 215, 458, 497
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 534
BstUI CGCG 3 cut(s) 174, 428, 525
BstV1I GCAGC 1 cut(s) 470
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 320
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 3 cut(s) 59, 249, 322
BtgI CCRYGG 1 cut(s) 172
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 2 cut(s) 232, 575
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 1 cut(s) 447
BtuMI TCGCGA 1 cut(s) 525
Cac8I GCNNGC 4 cut(s) 78, 220, 444, 475
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 3 cut(s) 76, 473, 479
Cfr42I CCGCGG 1 cut(s) 175
Csp6I GTAC 1 cut(s) 421
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 3 cut(s) 223, 268, 330
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 1 cut(s) 399
DpnI GATC 6 cut(s) 23, 38, 87, 186, 410, 432
DpnII GATC 6 cut(s) 21, 36, 85, 184, 408, 430
EaeI YGGCCR 2 cut(s) 57, 320
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 2 cut(s) 64, 577
EarI CTCTTC 2 cut(s) 64, 577
EclXI CGGCCG 1 cut(s) 320
Eco47III AGCGCT 2 cut(s) 75, 478
Eco52I CGGCCG 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 493
Eco81I CCTNAGG 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 519
EcoRII CCWGG 1 cut(s) 534
FaeI CATG 3 cut(s) 226, 271, 333
FatI CATG 3 cut(s) 222, 267, 329
FauI CCCGC 1 cut(s) 316
FbaI TGATCA 2 cut(s) 36, 184
Fnu4HI GCNGC 2 cut(s) 175, 459
FokI GGATG 2 cut(s) 239, 562
Fsp4HI GCNGC 2 cut(s) 175, 459
FspBI CTAG 1 cut(s) 480
GlaI GCGC 3 cut(s) 75, 472, 478
GluI GCNGC 2 cut(s) 175, 459
GsuI CTGGAG 2 cut(s) 49, 399
HaeII RGCGCY 2 cut(s) 77, 480
HaeIII GGCC 3 cut(s) 59, 249, 322
HapII CCGG 1 cut(s) 323
HhaI GCGC 3 cut(s) 76, 473, 479
Hin1II CATG 3 cut(s) 226, 271, 333
Hin6I GCGC 3 cut(s) 74, 471, 477
HinP1I GCGC 3 cut(s) 74, 471, 477
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 4 cut(s) 168, 316, 326, 341
HpaII CCGG 1 cut(s) 323
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 103, 284
Hpy188III TCNNGA 4 cut(s) 66, 89, 516, 524
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 139, 487
HpyCH4V TGCA 2 cut(s) 458, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 458, 497
HpyF3I CTNAG 1 cut(s) 399
Hsp92II CATG 3 cut(s) 226, 271, 333
HspAI GCGC 3 cut(s) 74, 471, 477
Ksp22I TGATCA 2 cut(s) 36, 184
KspI CCGCGG 1 cut(s) 175
Kzo9I GATC 6 cut(s) 21, 36, 85, 184, 408, 430
LguI GCTCTTC 1 cut(s) 64
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 480
MalI GATC 6 cut(s) 23, 38, 87, 186, 410, 432
MboI GATC 6 cut(s) 21, 36, 85, 184, 408, 430
MboII GAAGA 5 cut(s) 81, 145, 499, 564, 607
MflI RGATCY 1 cut(s) 430
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 6 cut(s) 26, 48, 275, 336, 436, 519
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 3 cut(s) 310, 320, 335
MnlI CCTC 4 cut(s) 157, 290, 311, 511
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 29, 549
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 3 cut(s) 174, 428, 525
MwoI GCNNNNNNNGC 3 cut(s) 215, 458, 497
NdeII GATC 6 cut(s) 21, 36, 85, 184, 408, 430
NlaIII CATG 3 cut(s) 226, 271, 333
NlaIV GGNNCC 1 cut(s) 432
NmeAIII GCCGAG 1 cut(s) 298
NruI TCGCGA 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 168
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 2 cut(s) 176, 460
PleI GAGTC 3 cut(s) 310, 320, 335
PpsI GAGTC 3 cut(s) 310, 320, 335
PshBI ATTAAT 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 430
RruI TCGCGA 1 cut(s) 525
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SacII CCGCGG 1 cut(s) 175
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 29, 549
SatI GCNGC 2 cut(s) 175, 459
Sau3AI GATC 6 cut(s) 21, 36, 85, 184, 408, 430
SchI GAGTC 3 cut(s) 310, 320, 335
ScrFI CCNGG 1 cut(s) 536
SetI ASST 5 cut(s) 203, 303, 372, 379, 515
SfaNI GCATC 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 175
Sse9I AATT 6 cut(s) 26, 48, 275, 336, 436, 519
SsiI CCGC 4 cut(s) 172, 174, 309, 428
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 139, 487
TaqI TCGA 1 cut(s) 344
TasI AATT 6 cut(s) 26, 48, 275, 336, 436, 519
TatI WGTACW 1 cut(s) 420
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 1 cut(s) 168
Tru1I TTAA 2 cut(s) 29, 549
Tru9I TTAA 2 cut(s) 29, 549
TscAI CASTG 1 cut(s) 454
TseI GCWGC 1 cut(s) 458
TspDTI ATGAA 1 cut(s) 564
TspRI CASTG 1 cut(s) 454
Van91I CCANNNNNTGG 1 cut(s) 31
VspI ATTAAT 1 cut(s) 29
XapI RAATTY 1 cut(s) 519
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.