Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0004244

Physical Location & Seq

Genomic Coordinates Forward (+)

22505552 .. 22512059

Gene Structure 6508 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0165000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3333 bp

ATGAGAGTAAAGTGGACGACAAAGCTCCACTGTCGCAGTTTGTTTAAAAGGTACTTTAGTAGTACTGAAATGGCGGCGGAGGAAGGCGGGAAGAGCTTTGCCAGGAGAGACCGTCTCCTACAGATTGAGGCAAAGGCTCGAAGCTGGTGGGAAGACAAACAAGTTTTCAGGGCCCAGTCTCGTGCAGAGCCGCCCGAGCCAGGGGAGAAGTTCTTTGGGAACTTCCCCTTCCCTTATATGAACGGTATTCTGCATCTCGGACATGCTTTCTCACTGTCGAAGCTGGAGTTTGCTGCAGCCTATCATCGATTGAGAGGCGCAAATGTCCTCTTGCCTTTTGCTTTTCACTGTACTGGCATGCCCATCAAAGCCTCTGCCGATAAGCTTATTAGGGAGATTCAGCAGTTTGGAGAACCTCCTGTTTTTCCCAAAAAAGTGGAGAAAGGGAACGAGGAAGTGGAAGCGGAAGCAGAGGATGTCAATGCAGGCGAACCTCCAGCCAAGCATAAGGGAAAGAAGTCTAAGGCTGCAGCAAAATCAAGCGGCGAAGTGTACCAATGGGAAATTATGAAGAGCTTTGGCCTCTCCGACAGTGACATATCCAAATTTCAGGACCCTTATAAGTGGTTGACTTATTTTCCCCCATTGGCAGTGGAGGACCTCAAGGCTTTTGGCCTGGGCTGTGACTGGAGACGTTCCTTTATTACTACAGACATGAACCCTTTTTTCGACACCTTTGTGAGGTGGCAGGTTAGGAAACTGAAATCCATGGGTAAGATTGTCAAAGATCTCCGTTACACGATATACTCTCCGATGGATGGGCAACCCTGTGCAGATCATGATAGGTCCAGTGGTGAAGGAGTTCAGCCCCAAGAATATACTGTCATCAAAATGGAACTGATGGCACCTTTTCCTTCTAAATTGGGAGTGTTGGAGGGGAGGAAAGTGTTCCTTGCTGCAGCAACACTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCTGATGGTAAGTATGGTGCATATGAAATCAATGAAACAGATGTGTTCATTCTTACACAGAGAGCAGCACGTAATCTTGCTTATCAAAAGTACTCTAGGGTCCCAGAGAAACCTACTTGCTTGGTTGAACTGACTGGTTACGATTTGATTGGCCTTCCATTGAAGTCGCCACTAGCAGTGAATCAGATTATTTATGCTCTTCCTATGTTGACTGTCCTAACAGACAAAGGTACTGGGATTGTGACCAGTGTACCTACTGATTCTCCGGATGACTATATGGCCTTGCATGACTTGAAATCAAAACCTGCTTTTAGGGCAAAATATGGTGTGAAAGATGAGTGGGTACTGCCCTTTGAGATTATCCCAATCATTGATATTCCAGGATATGGAAATAAGGCTGCAGAAAAGGTCTGTGCAGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAAGTTGACATACTTGAAAGGATTCAATGAAGGAACACTGATTGTGGGGGAATTTAAGGGAAGGAAAGTCCAGGAGGTTAAGCCCTTGATAAGGAGCAAGCTTATAGAGGCGGGGGAGGCAATATCATACAGTGAACCTGAGAAGCGGGTACTATCACGATCAGGTGATGAATGTGTTGTGGCGCTTACAGATCAATGGTACATCACTTACGGGGAAACTGAATGGAAAAAACTGGCTGAGGAGTGCTTGTCCAGCATGAGTTTGTATTCTGATGAGACACAGCATGGATTTGAACACACATTGAGCTGGTTGAATCAGTGGGCTTGTTCACGATCTTTTGGTCTTGGGACTCGCATCCCCTGGGATGAAGAATTCTTAGTTGAGTCATTGTCTGATTCAACTATTTACATGGCTTATTACACCATTGCTCACTTCCTACACAATGGAGACATGTATGGTTCCAGCAAATCTGCAATTAAACCTGAACAAATGACTGATGAGGTCTGGGATTTTATTTTCTGTGATGGCCCATATCCTAAATCATCCGACATCTCATCATCAATTCTGAGTGAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTCGGGTGTCTGGCAAGGATCTAATACAAAATCATTTAACCTTCTGCATCTACAACCATACAGCTATTATGTCCAAGAAGCACTGGCCTCGTGGTTTCAGATGCAATGGGCACCTACTGCTCAATGCTCGGAAAATGGCCAAGTCCACAGGAAACTTTAGGACAATTCGCCAGGCAATTGAGGAATTTTCTGCTGATGCCACTCGCTTTTCTTTGGCTGATGCTGGAGATGGTATTGATGATGCAAATTTTGCATTTAACACTGCCAATGCTGCAATCCTGCGGCTCACTAAAGAGATCTCATGGATGGAAGAAGTTTTAGGTACAGACTCTTCTTTGAGACCAGGACCCCCAACTACTTATGCTGACAGGGTCTTTGCAAATGAGATAAATATTGCTGTGAACAGGACTGAGCAGAATTACAGCAACTGCATGTTCCGAGAAGCCCTCAAGACTGGATTTTATGATCTTCAAGCTGCTAGGGATGAGTATCAGTTTTCATGTGGTGCTGGGGGAATGAACCGTGATTTGCTGTGGCATTTTATGGATGTGCAGACACGTCTTATTACTCCAATCTGCCCACACTATGGAGAATACGTATGGAGGGAACTTTTGAAGAAGGACGGCTTTGTGGTAAACGCAGGATGGCCTTTGGCTGACGCTCCGGACCTAACCCTCCAGAGTGCCAATAAGTATTTGCAGGACTCAATCATTTCAATGAGGAAGCTCCTCCAAAAGCAAGTTTCAAGCTCAAAGAAGGGTAAGAAGGGTACTCCAGTCACAGCGTTGACAGAAGACAACAATCTGACCGGCTTAATCTATGTGAACGAGCACTTTGATGGATGGAAGGCAGAGTGCCTGAAAATCCTCCAAATTAACTTTAACAGAGACAGTCATACTTTTTCTTCAGACAAGGTGATACAAGAAGCGTTGCAAAAAAGTTCTATTGGTCAGTCGAAAGATTTTAGACAAATCCAAAAGCTTTGTATGCCTTTCATGAAGTTCAAGAAGGATGAGGCAATTGCAATTGGGGATCAGGCCTTGGATTTGAAGCTACCTTTTGGCGAGATTGAGGTCCTTAAAGAGAACTTGGACTTAATTAAGAGACAACTTGGTCTTCAAGAGGTAGAAATATTGTCTGCTACAAATCCTGATGCACTTTCTAAAGCCGGTTCTCTTGTCAAACTGTTAGAGCAGAATCCTCCAGCACCCGGAAGCCCTACTGCCATCTTCTTGACCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016192 GO:0016197 GO:0019538 GO:0019941 GO:0030163 GO:0031410 GO:0031982 GO:0032509 GO:0032511 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043328 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0045324 GO:0046907 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Ist1

Protein Analysis

1110

Amino Acids

125.71

Weight (kDa)

6.62

Isoelectric Point (pI)

43.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	48 - 130	3e-08	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1	PF00133	217 - 779	4.9e-15	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	687 - 785	5e-07	tRNA synthetases class I (M)
tRNA-synt_1e	PF01406	693 - 769	1.6e-06	tRNA synthetases class I (C) catalytic domain
Anticodon_1	PF08264	819 - 943	2.2e-13	Anticodon-binding domain of tRNA ligase
RBD_LARS1	PF24810	972 - 1048	1.3e-06	Leucine--tRNA ligase, RagD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004244)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g08370 FvH4_7g08383 FvH4_7g08390
malus_domestica	MD07G1084100.v1.1
prunus_persica	Prupe.2G111000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0340191 RchiOBHm_Chr1g0340211 RchiOBHm_Chr1g0340251
rosa_laevigata	RLG00000029004 RLG00000029005 RLG00000029007
rosa_multiflora	Rmu_co8401649.1_g000001 Rmu_sc0001836.1_g000001 Rmu_sc0001836.1_g000002 Rmu_sc0001836.1_g000005 Rmu_sc0005193.1_g000011 Rmu_sc0005193.1_g000016 Rmu_sc0005193.1_g000018
rosa_roxburghii	Rroxscaffold_4G00311090
rosa_rugosa	Rorug01G0164700.1 Rorug01G0165000 Rorug01G0165100
rosa_samantha	Rh1AG179300 Rh1AG179700 Rh1BG147500 Rh1BG147700 Rh1BG147900 Rh1CG166200 Rh1DG179400 Rh1DG179500 Rh1DG179900
rosa_wichuraiana	Rw1G014940 Rw1G014960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	621
AasI	GACNNNNNNGTC	1 cut(s)	3201
Acc36I	ACCTGC	2 cut(s)	739, 1324
AccB1I	GGYRCC	2 cut(s)	904, 2199
AccB7I	CCANNNNNTGG	2 cut(s)	1397, 2675
AccIII	TCCGGA	2 cut(s)	1276, 2752
AciI	CCGC	9 cut(s)	74, 77, 87, 191, 464, 543, 1589, 1624, 2371
AclWI	GGATC	2 cut(s)	2115, 3129
AcoI	YGGCCR	1 cut(s)	2226
AcsI	RAATTY	5 cut(s)	605, 1529, 1850, 2273, 2335
AcuI	CTGAAG	1 cut(s)	2979
AfiI	CCNNNNNNNGG	8 cut(s)	200, 201, 741, 818, 1397, 1569, 2675, 3299
AflIII	ACRYGT	2 cut(s)	1929, 2645
AjiI	CACGTC	1 cut(s)	2648
AjnI	CCWGG	9 cut(s)	101, 199, 675, 1390, 1548, 1838, 2259, 2431, 3326
AleI	CACNNNNGTG	1 cut(s)	737
Alw21I	GWGCWC	1 cut(s)	2922
AlwI	GGATC	2 cut(s)	2115, 3129
AlwNI	CAGNNNCTG	1 cut(s)	2517
Ama87I	CYCGRG	1 cut(s)	194
Aor13HI	TCCGGA	2 cut(s)	1276, 2752
ApaI	GGGCCC	1 cut(s)	175
ApoI	RAATTY	5 cut(s)	605, 1529, 1850, 2273, 2335
ArsI	GACNNNNNNTTYG	2 cut(s)	2447, 2479
Asp700I	GAANNNNTTC	3 cut(s)	861, 947, 1499
AspLEI	GCGC	2 cut(s)	320, 1663
AsuC2I	CCSGG	1 cut(s)	3300
AsuHPI	GGTGA	3 cut(s)	866, 1655, 3016
AsuNHI	GCTAGC	1 cut(s)	1465
AvaI	CYCGRG	1 cut(s)	194
AvaII	GGWCC	7 cut(s)	613, 658, 846, 1111, 2435, 2755, 3163
BaeGI	GKGCMC	2 cut(s)	175, 2202
BaeI	ACNNNNGTAYC	6 cut(s)	43, 76, 1245, 1278, 2850, 2883
BalI	TGGCCA	1 cut(s)	2228
BanI	GGYRCC	2 cut(s)	904, 2199
BanII	GRGCYC	1 cut(s)	175
BarI	GAAGNNNNNNTAC	4 cut(s)	1472, 1504, 2404, 2436
BauI	CACGAG	2 cut(s)	180, 2178
BbsI	GAAGAC	3 cut(s)	159, 2889, 3197
Bbv12I	GWGCWC	1 cut(s)	2922
BbvCI	CCTCAGC	1 cut(s)	1716
BceAI	ACGGC	1 cut(s)	2728
BcgI	CGANNNNNNTGC	2 cut(s)	2159, 2193
BciT130I	CCWGG	9 cut(s)	103, 201, 677, 1392, 1550, 1840, 2261, 2433, 3328
BciVI	GTATCC	1 cut(s)	2088
BcnI	CCSGG	1 cut(s)	3300
BfaI	CTAG	4 cut(s)	1107, 1184, 1466, 2568
BfmI	CTRYAG	6 cut(s)	119, 294, 528, 708, 957, 1410
BfoI	RGCGCY	1 cut(s)	1664
BfuAI	ACCTGC	2 cut(s)	739, 1324
BfuI	GTATCC	1 cut(s)	2088
BglII	AGATCT	3 cut(s)	787, 1429, 2385
BmcAI	AGTACT	2 cut(s)	64, 1103
Bme18I	GGWCC	7 cut(s)	613, 658, 846, 1111, 2435, 2755, 3163
BmeT110I	CYCGRG	1 cut(s)	194
BmgBI	CACGTC	1 cut(s)	2648
BmiI	GGNNCC	8 cut(s)	173, 615, 906, 1112, 1113, 1939, 2201, 2437
BmrI	ACTGGG	2 cut(s)	169, 1254
BmsI	GCATC	8 cut(s)	262, 1842, 2145, 2180, 2275, 2299, 2320, 3232
BmtI	GCTAGC	1 cut(s)	1469
BmuI	ACTGGG	2 cut(s)	169, 1254
BpiI	GAAGAC	3 cut(s)	159, 2889, 3197
BplI	GAGNNNNNCTC	4 cut(s)	99, 131, 2520, 2552
BpmI	CTGGAG	7 cut(s)	305, 480, 709, 2334, 2750, 2847, 3276
Bpu10I	CCTNAGC	1 cut(s)	1716
BpuEI	CTTGAG	3 cut(s)	647, 1418, 2522
BpuMI	CCSGG	1 cut(s)	3300
Bsa29I	ATCGAT	1 cut(s)	307
BsaAI	YACGTR	2 cut(s)	1082, 2686
BsaBI	GATNNNNATC	1 cut(s)	2577
BsaI	GGTCTC	4 cut(s)	102, 964, 971, 2422
BsaJI	CCNNGG	6 cut(s)	200, 676, 768, 1838, 1839, 3129
BsaWI	WCCGGW	2 cut(s)	1276, 2752
BsaXI	ACNNNNNCTCC	8 cut(s)	97, 127, 1258, 1288, 1712, 1742, 2670, 2700
Bsc4I	CCNNNNNNNGG	8 cut(s)	200, 201, 741, 818, 1397, 1569, 2675, 3299
Bse118I	RCCGGY	2 cut(s)	2897, 3257
Bse3DI	GCAATG	2 cut(s)	1902, 2200
Bse8I	GATNNNNATC	1 cut(s)	2577
BseAI	TCCGGA	2 cut(s)	1276, 2752
BseBI	CCWGG	9 cut(s)	103, 201, 677, 1392, 1550, 1840, 2261, 2433, 3328
BseCI	ATCGAT	1 cut(s)	307
BseDI	CCNNGG	6 cut(s)	200, 676, 768, 1838, 1839, 3129
BseJI	GATNNNNATC	1 cut(s)	2577
BseLI	CCNNNNNNNGG	8 cut(s)	200, 201, 741, 818, 1397, 1569, 2675, 3299
BseMI	GCAATG	2 cut(s)	1902, 2200
BseMII	CTCAG	6 cut(s)	959, 966, 1608, 1707, 2036, 2490
BseRI	GAGGAG	2 cut(s)	1733, 2807
BseSI	GKGCMC	2 cut(s)	175, 2202
BseYI	CCCAGC	1 cut(s)	2597
BsgI	GTGCAG	4 cut(s)	204, 852, 1446, 2660
BshNI	GGYRCC	2 cut(s)	904, 2199
BshVI	ATCGAT	1 cut(s)	307
BsiHKAI	GWGCWC	1 cut(s)	2922
BsiHKCI	CYCGRG	1 cut(s)	194
BsiSI	CCGG	5 cut(s)	1277, 2753, 2898, 3258, 3300
BslFI	GGGAC	2 cut(s)	1097, 1840
BslI	CCNNNNNNNGG	8 cut(s)	200, 201, 741, 818, 1397, 1569, 2675, 3299
BsmBI	CGTCTC	2 cut(s)	119, 685
BsmFI	GGGAC	2 cut(s)	1097, 1840
Bso31I	GGTCTC	4 cut(s)	102, 964, 971, 2422
BsoBI	CYCGRG	1 cut(s)	194
Bsp120I	GGGCCC	1 cut(s)	171
Bsp1286I	GDGCHC	3 cut(s)	175, 2202, 2922
Bsp13I	TCCGGA	2 cut(s)	1276, 2752
Bsp19I	CCATGG	1 cut(s)	768
BspACI	CCGC	9 cut(s)	74, 77, 87, 191, 464, 543, 1589, 1624, 2371
BspCNI	CTCAG	6 cut(s)	960, 967, 1609, 1708, 2037, 2491
BspDI	ATCGAT	1 cut(s)	307
BspEI	TCCGGA	2 cut(s)	1276, 2752
BspHI	TCATGA	2 cut(s)	838, 3084
BspLI	GGNNCC	8 cut(s)	173, 615, 906, 1112, 1113, 1939, 2201, 2437
BspMAI	CTGCAG	4 cut(s)	298, 532, 961, 1414
BspMI	ACCTGC	2 cut(s)	739, 1324
BspOI	GCTAGC	1 cut(s)	1469
BspPI	GGATC	2 cut(s)	2115, 3129
BspQI	GCTCTTC	3 cut(s)	86, 566, 1215
BspT107I	GGYRCC	2 cut(s)	904, 2199
BspTNI	GGTCTC	4 cut(s)	102, 964, 971, 2422
BsrDI	GCAATG	2 cut(s)	1902, 2200
BsrFI	RCCGGY	2 cut(s)	2897, 3257
BssAI	RCCGGY	2 cut(s)	2897, 3257
BssECI	CCNNGG	6 cut(s)	200, 676, 768, 1838, 1839, 3129
BssSI	CACGAG	2 cut(s)	180, 2178
BssT1I	CCWWGG	2 cut(s)	768, 3129
Bst2BI	CACGAG	2 cut(s)	180, 2178
Bst2UI	CCWGG	9 cut(s)	103, 201, 677, 1392, 1550, 1840, 2261, 2433, 3328
Bst6I	CTCTTC	4 cut(s)	86, 566, 1215, 2425
BstAPI	GCANNNNNTGC	2 cut(s)	2206, 2339
BstBAI	YACGTR	2 cut(s)	1082, 2686
BstC8I	GCNNGC	4 cut(s)	359, 487, 1467, 1577
BstDEI	CTNAG	8 cut(s)	522, 968, 975, 1617, 1716, 1855, 2045, 2499
BstDSI	CCRYGG	1 cut(s)	768
BstENI	CCTNNNNNAGG	2 cut(s)	739, 1567
BstH2I	RGCGCY	1 cut(s)	1664
BstHHI	GCGC	2 cut(s)	320, 1663
BstMWI	GCNNNNNNNGC	9 cut(s)	93, 196, 1325, 1595, 1731, 2206, 2339, 2360, 3076
BstNI	CCWGG	9 cut(s)	103, 201, 677, 1392, 1550, 1840, 2261, 2433, 3328
BstNSI	RCATGY	4 cut(s)	266, 361, 1933, 2524
BstSFI	CTRYAG	6 cut(s)	119, 294, 528, 708, 957, 1410
BstSLI	GKGCMC	2 cut(s)	175, 2202
BstSNI	TACGTA	1 cut(s)	2686
BstV2I	GAAGAC	3 cut(s)	159, 2889, 3197
BstX2I	RGATCY	4 cut(s)	787, 1429, 2107, 2385
BstXI	CCANNNNNNTGG	1 cut(s)	436
BstYI	RGATCY	4 cut(s)	787, 1429, 2107, 2385
Bsu15I	ATCGAT	1 cut(s)	307
BsuI	GTATCC	1 cut(s)	2088
BsuTUI	ATCGAT	1 cut(s)	307
BtgI	CCRYGG	1 cut(s)	768
BtrI	CACGTC	1 cut(s)	2648
BtsI	GCAGTG	3 cut(s)	657, 1194, 2349
BveI	ACCTGC	2 cut(s)	739, 1324
Cac8I	GCNNGC	4 cut(s)	359, 487, 1467, 1577
CaiI	CAGNNNCTG	1 cut(s)	2517
CciI	TCATGA	2 cut(s)	838, 3084
CfoI	GCGC	2 cut(s)	320, 1663
Cfr10I	RCCGGY	2 cut(s)	2897, 3257
ClaI	ATCGAT	1 cut(s)	307
CseI	GACGC	1 cut(s)	2756
CsiI	ACCWGGT	1 cut(s)	3326
DdeI	CTNAG	8 cut(s)	522, 968, 975, 1617, 1716, 1855, 2045, 2499
DraI	TTTAAA	1 cut(s)	46
DrdI	GACNNNNNNGTC	1 cut(s)	3201
DseDI	GACNNNNNNGTC	1 cut(s)	3201
EaeI	YGGCCR	1 cut(s)	2226
Eam1104I	CTCTTC	4 cut(s)	86, 566, 1215, 2425
EarI	CTCTTC	4 cut(s)	86, 566, 1215, 2425
EciI	GGCGGA	1 cut(s)	92
Eco105I	TACGTA	1 cut(s)	2686
Eco130I	CCWWGG	2 cut(s)	768, 3129
Eco147I	AGGCCT	1 cut(s)	3128
Eco24I	GRGCYC	1 cut(s)	175
Eco31I	GGTCTC	4 cut(s)	102, 964, 971, 2422
Eco47I	GGWCC	7 cut(s)	613, 658, 846, 1111, 2435, 2755, 3163
Eco57I	CTGAAG	1 cut(s)	2979
Eco88I	CYCGRG	1 cut(s)	194
EcoNI	CCTNNNNNAGG	2 cut(s)	739, 1567
EcoO109I	RGGNCCY	6 cut(s)	171, 613, 658, 1111, 2435, 3163
EcoRI	GAATTC	1 cut(s)	1850
EcoRII	CCWGG	9 cut(s)	101, 199, 675, 1390, 1548, 1838, 2259, 2431, 3326
EcoT14I	CCWWGG	2 cut(s)	768, 3129
EcoT22I	ATGCAT	1 cut(s)	1003
EcoT38I	GRGCYC	1 cut(s)	175
ErhI	CCWWGG	2 cut(s)	768, 3129
Esp3I	CGTCTC	2 cut(s)	119, 685
FalI	AAGNNNNNCTT	4 cut(s)	936, 968, 2699, 2731
FaqI	GGGAC	2 cut(s)	1097, 1840
FauI	CCCGC	3 cut(s)	80, 1582, 1617
FauNDI	CATATG	1 cut(s)	1033
FriOI	GRGCYC	1 cut(s)	175
FspBI	CTAG	4 cut(s)	1107, 1184, 1466, 2568
GlaI	GCGC	2 cut(s)	319, 1662
GsaI	CCCAGC	1 cut(s)	2601
GsuI	CTGGAG	7 cut(s)	305, 480, 709, 2334, 2750, 2847, 3276
HaeII	RGCGCY	1 cut(s)	1664
HapII	CCGG	5 cut(s)	1277, 2753, 2898, 3258, 3300
HgaI	GACGC	1 cut(s)	2756
HhaI	GCGC	2 cut(s)	320, 1663
Hin6I	GCGC	2 cut(s)	318, 1661
HinP1I	GCGC	2 cut(s)	318, 1661
HincII	GTYRAC	4 cut(s)	630, 1221, 1485, 2877
HindII	GTYRAC	4 cut(s)	630, 1221, 1485, 2877
HindIII	AAGCTT	3 cut(s)	383, 1577, 3068
HpaII	CCGG	5 cut(s)	1277, 2753, 2898, 3258, 3300
HphI	GGTGA	3 cut(s)	866, 1655, 3016
HpyCH4IV	ACGT	4 cut(s)	694, 1081, 2647, 2685
HpyF10VI	GCNNNNNNNGC	9 cut(s)	93, 196, 1325, 1595, 1731, 2206, 2339, 2360, 3076
HpyF3I	CTNAG	8 cut(s)	522, 968, 975, 1617, 1716, 1855, 2045, 2499
HpySE526I	ACGT	4 cut(s)	694, 1081, 2647, 2685
HspAI	GCGC	2 cut(s)	318, 1661
KflI	GGGWCCC	1 cut(s)	1111
Kpn2I	TCCGGA	2 cut(s)	1276, 2752
LguI	GCTCTTC	3 cut(s)	86, 566, 1215
LmnI	GCTCC	4 cut(s)	30, 1572, 2755, 2820
LweI	GCATC	8 cut(s)	262, 1842, 2145, 2180, 2275, 2299, 2320, 3232
MabI	ACCWGGT	1 cut(s)	3326
MaeI	CTAG	4 cut(s)	1107, 1184, 1466, 2568
MaeII	ACGT	4 cut(s)	694, 1081, 2647, 2685
MaeIII	GTNAC	6 cut(s)	593, 683, 794, 1148, 1252, 2866
MfeI	CAATTG	3 cut(s)	2265, 3108, 3114
MflI	RGATCY	4 cut(s)	787, 1429, 2107, 2385
MhlI	GDGCHC	3 cut(s)	175, 2202, 2922
MlsI	TGGCCA	1 cut(s)	2228
MluNI	TGGCCA	1 cut(s)	2228
MlyI	GAGTC	4 cut(s)	1822, 1871, 2411, 2786
MmeI	TCCRAC	3 cut(s)	612, 912, 2049
Mox20I	TGGCCA	1 cut(s)	2228
Mph1103I	ATGCAT	1 cut(s)	1003
MroI	TCCGGA	2 cut(s)	1276, 2752
MroXI	GAANNNNTTC	3 cut(s)	861, 947, 1499
MscI	TGGCCA	1 cut(s)	2228
MslI	CAYNNNNRTG	4 cut(s)	737, 890, 2804, 2925
Msp20I	TGGCCA	1 cut(s)	2228
MspI	CCGG	5 cut(s)	1277, 2753, 2898, 3258, 3300
MunI	CAATTG	3 cut(s)	2265, 3108, 3114
MvaI	CCWGG	9 cut(s)	103, 201, 677, 1392, 1550, 1840, 2261, 2433, 3328
MwoI	GCNNNNNNNGC	9 cut(s)	93, 196, 1325, 1595, 1731, 2206, 2339, 2360, 3076
NciI	CCSGG	1 cut(s)	3300
NcoI	CCATGG	1 cut(s)	768
NdeI	CATATG	1 cut(s)	1033
NheI	GCTAGC	1 cut(s)	1465
NlaIV	GGNNCC	8 cut(s)	173, 615, 906, 1112, 1113, 1939, 2201, 2437
NmuCI	GTSAC	4 cut(s)	593, 683, 1252, 2866
NsiI	ATGCAT	1 cut(s)	1003
NspI	RCATGY	4 cut(s)	266, 361, 1933, 2524
OliI	CACNNNNGTG	1 cut(s)	737
PacI	TTAATTAA	1 cut(s)	3189
PaeI	GCATGC	1 cut(s)	361
PagI	TCATGA	2 cut(s)	838, 3084
PasI	CCCWGGG	1 cut(s)	1839
PceI	AGGCCT	1 cut(s)	3128
PciI	ACATGT	1 cut(s)	1929
PciSI	GCTCTTC	3 cut(s)	86, 566, 1215
PdmI	GAANNNNTTC	3 cut(s)	861, 947, 1499
PfeI	GAWTC	7 cut(s)	397, 1192, 1271, 1500, 1792, 1874, 3286
PflFI	GACNNNGTC	1 cut(s)	2459
PflMI	CCANNNNNTGG	2 cut(s)	1397, 2675
PfoI	TCCNGGA	2 cut(s)	1390, 1548
PleI	GAGTC	4 cut(s)	1822, 1870, 2411, 2786
PpsI	GAGTC	4 cut(s)	1822, 1870, 2411, 2786
Ppu21I	YACGTR	2 cut(s)	1082, 2686
PpuMI	RGGWCCY	5 cut(s)	613, 658, 1111, 2435, 3163
PscI	ACATGT	1 cut(s)	1929
PsiI	TTATAA	1 cut(s)	621
Psp5II	RGGWCCY	5 cut(s)	613, 658, 1111, 2435, 3163
Psp6I	CCWGG	9 cut(s)	101, 199, 675, 1390, 1548, 1838, 2259, 2431, 3326
PspFI	CCCAGC	1 cut(s)	2597
PspGI	CCWGG	9 cut(s)	101, 199, 675, 1390, 1548, 1838, 2259, 2431, 3326
PspN4I	GGNNCC	8 cut(s)	173, 615, 906, 1112, 1113, 1939, 2201, 2437
PspOMI	GGGCCC	1 cut(s)	171
PspPPI	RGGWCCY	5 cut(s)	613, 658, 1111, 2435, 3163
PstI	CTGCAG	4 cut(s)	298, 532, 961, 1414
PstNI	CAGNNNCTG	1 cut(s)	2517
PsuI	RGATCY	4 cut(s)	787, 1429, 2107, 2385
PsyI	GACNNNGTC	1 cut(s)	2459
RseI	CAYNNNNRTG	4 cut(s)	737, 890, 2804, 2925
SapI	GCTCTTC	3 cut(s)	86, 566, 1215
ScaI	AGTACT	2 cut(s)	64, 1103
SchI	GAGTC	4 cut(s)	1822, 1871, 2411, 2786
SduI	GDGCHC	3 cut(s)	175, 2202, 2922
SexAI	ACCWGGT	1 cut(s)	3326
SfaNI	GCATC	8 cut(s)	262, 1842, 2145, 2180, 2275, 2299, 2320, 3232
SfcI	CTRYAG	6 cut(s)	119, 294, 528, 708, 957, 1410
SinI	GGWCC	7 cut(s)	613, 658, 846, 1111, 2435, 2755, 3163
SmiMI	CAYNNNNRTG	4 cut(s)	737, 890, 2804, 2925
SmlI	CTYRAG	3 cut(s)	662, 1433, 2537
SmoI	CTYRAG	3 cut(s)	662, 1433, 2537
SnaBI	TACGTA	1 cut(s)	2686
SphI	GCATGC	1 cut(s)	361
SseBI	AGGCCT	1 cut(s)	3128
SsiI	CCGC	9 cut(s)	74, 77, 87, 191, 464, 543, 1589, 1624, 2371
SspI	AATATT	3 cut(s)	2072, 2482, 3222
SspMI	CTAG	4 cut(s)	1107, 1184, 1466, 2568
StuI	AGGCCT	1 cut(s)	3128
StyI	CCWWGG	2 cut(s)	768, 3129
TaiI	ACGT	4 cut(s)	697, 1084, 2650, 2688
TaqI	TCGA	5 cut(s)	139, 278, 307, 729, 3044
TatI	WGTACW	3 cut(s)	62, 350, 1101
TauI	GCSGC	4 cut(s)	77, 193, 546, 2374
TfiI	GAWTC	7 cut(s)	397, 1192, 1271, 1500, 1792, 1874, 3286
TseFI	GTSAC	4 cut(s)	593, 683, 1252, 2866
Tsp45I	GTSAC	4 cut(s)	593, 683, 1252, 2866
TspGWI	ACGGA	1 cut(s)	782
Tth111I	GACNNNGTC	1 cut(s)	2459
Van91I	CCANNNNNTGG	2 cut(s)	1397, 2675
VpaK11BI	GGWCC	7 cut(s)	613, 658, 846, 1111, 2435, 2755, 3163
XagI	CCTNNNNNAGG	2 cut(s)	739, 1567
XapI	RAATTY	5 cut(s)	605, 1529, 1850, 2273, 2335
XceI	RCATGY	4 cut(s)	266, 361, 1933, 2524
XmnI	GAANNNNTTC	3 cut(s)	861, 947, 1499
XspI	CTAG	4 cut(s)	1107, 1184, 1466, 2568
ZrmI	AGTACT	2 cut(s)	64, 1103
Zsp2I	ATGCAT	1 cut(s)	1003

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.