Rorug01G0165100

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000302
Physical Location & Seq
Forward (+)
22543228 .. 22549075
5848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0165100.1

Sequence Viewer

Length: 2487 bp
ATGACGGACTTTGAGCCGCTGCAACAGAAGCCGGAATCGGCCGATGCCTGCGGTGATTTCGAGACTGGATATGAGGAATTCATGCGGGGGCACTTGGATGAATGTATGTCATTTGCTTCATGTAGCTCTCCGCGGAATCCTGATGATGAGGATGATGAGGGGGAGCAGCTTGTTCGGAGGAGACGGAGGTTGGATTTGGAGGGGGATGATTTGGCCGAGTCGTCTGCTGCTGCGCGGCGCCACCACTCGCGGATTTTGACCCGTTGGGCGGCGCGGCAGGCGCAGGAGATGATAACCACCATTGAGAGGAGGAATCGGGAGTCGGAGCTGATGGCGCTTGCGGGGTTGCATACGGTTTCGATGCTCGACTCCTCCTTCTTGAGGGAGTCGCAGTCCCCTACGTCGAGGCGGCAGGGGACTGTGGAGAGGCCGAGCACTCAGGCGTCTGCAATTTTGCAAATGTGGCGGGAGCTGGAGGATGAGCATTTGTTGAATAGAGCACGCGGGAGGGTGAGGGAAAGGTTGAGGCAGCAGAGGAATGTTGAGTCTAATACTAATGAGTTGAACACAAATAGATCTGATAGTCGGGGGAGTGAGAATCCTGGGAGTTTGGTGGATGCGAGTGAGAGTGAAAATGAGTATGGGACGTGGTCACATGATCAGATGGGTTCACCAAACGAACGAGGTGATAATAATGCATCTAGCAGGGAGCAATCTCCCGATCTTGGCGAAGTTGAGAGGGAGAGAGTGAGGCAGATTGTACGGGGATGGATGGAAACTGGCATTACTGATCATTCATCCAATGTTGCTCCAAGAAATAGTAGTCCTAGGGCAGAGTGGCTTGGTGAGACGGAGCGGGAAAGGGTGAGAATCGTCCGGGAGTGGGTGCAAATGGCTAGTCAGCAGAGAGGAGGTCGTGGAGGCCGACGGGAAGATCATGTCACCAATGGTGGTGCTCAAGCTGGTATAGTGCGTGATGAGTCAGCTGCTGACCAAGAAGAGAGCCAACCAGAGCACATTCGCCGGGATATGTTGAGGTTGCGTGGAAGACAAGCTATAATTGATTTGCTTGTGAGAATTGAGAGGGAAAGACAAAGGGAGCTCCAGTCTTTGGTGGAGCATCGAGCTGTCTCTGATTTTGCTCATCGCAACCGCATTCAGTCATTACTCAGAGGTAGATTTTTGCGAAATGAAAGGCCTATTGAAGAAGAAAGACCACCTTCTATAGCCGCGGGTGAATTAGTCCAATTGAGGCAGAGACACACTGTTTCTGGTTTAAGAAGCGATCATACCCAAACAATCGCTTCTCAGGATATTCAGCAGGAAAACCGTGAGCAATCCCAGCCTGGAAGCGAGGGAGGTGACAATCAGTTCCCTAATCAATTGGGGAACTTGGAAAATGACACAGCTGTCGAGCGACTTGATTGGCAAGAAACTGCTAATCAAGGTGGGAATTGGCAGGAACCAATTGCTGAAGATGAGAGACGTAACTGGGAGCAGACAACTTTTGATCAATTTAACGATTGGAGGGAAGGTAATGCTGAAGATATAGTTGGAAATTGGCCTCAGGGAACTGCGCGAAATGCTGATGAAGAAACAGGTCATCAACAAGAAGCTCAGGGTACATGGCGTGAGAATAGTTCTCGGGAAGCTGTTGAGAATTGGTCAGAAAGACCTTCAGGTCCTGTGAGAAATCGTCGTTCTGGCTCAGTTAGAAGATTCAATAGATTTAGTCCACCAGATGATGATACCGTGTACAGTATGGAACTGAGGGAACTTCTGAGCAGGAGAAGTGTTTCGAATCTTCTTCGTAGTGGATTTCGTGAAAGTCTGGACCAGCTGATACAATCATATGTTGAAAGGCAAAGTCATGCTCCCATTGAGTGGGATTTGCATAGAAACTTGCCAACACCAACTCCGGCTTCACCAGTGCAGGACCAGGACCGACAGAGGGATGATCTGAATGAAGATCAGCATGATGCCATCAACAGACCTTCACGTGTTCTACCATCTCCACCAGTTCCCCCACCACAACCATTATGGCATCAGGACTTGCATAACACTGGCTGGTCTCGCCATAGCATGCATCGTTCAGAAATTGAATGGGAGATGTTTAATGATCTCAGATCAGACATGGCAAGACTCCAGCAAGGCATGAGCCACATGCAAAGGATGTTGGAGGCCTGCATGGATATGCAATTAGAGTTGCAACGTTCTGTCAGACAGGAAGTCTCGGCGGCTCTCAATCGTTCGCCTGGTGAAAAAGGGTCAGGTGCTGAGACATCAGAGGATGGTTCTAAATGGGGTCATGTAAGAAAAGGAACTTGTTGTGTTTGTTGTGATAGTCACATCGATTCTCTCTTGTATAGATGCGGGCATATGTGTACATGTTCAAAATGTGCAAACGAATTGGTTCGTGGTGGAGGCAAGTGCCCACTTTGTCGAGCGCCTATAGTTGAGGTGATCCGTGCATACTCCATACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

828

Amino Acids

94.98

Weight (kDa)

5.43

Isoelectric Point (pI)

70.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 773 - 820 5.5e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1409, 1680
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 2 cut(s) 1111, 1884
AccBSI CCGCTC 1 cut(s) 856
AccII CGCG 7 cut(s) 133, 235, 250, 274, 504, 1232, 1579
AclI AACGTT 1 cut(s) 2212
AclWI GGATC 1 cut(s) 2458
AcoI YGGCCR 2 cut(s) 39, 213
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 3 cut(s) 1494, 1563, 1662
AcvI CACGTG 1 cut(s) 2000
AcyI GRCGYC 2 cut(s) 238, 443
AfaI GTAC 4 cut(s) 762, 1624, 1757, 2386
AfiI CCNNNNNNNGG 8 cut(s) 268, 306, 381, 725, 883, 1111, 1884, 1951
AflIII ACRYGT 2 cut(s) 1999, 2387
AgsI TTSAA 7 cut(s) 493, 565, 1205, 1723, 1859, 2102, 2394
AhdI GACNNNNNGTC 1 cut(s) 2228
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 4 cut(s) 601, 1345, 1938, 2254
AjuI GAANNNNNNNTTGG 4 cut(s) 1536, 1568, 1906, 1938
Alw21I GWGCWC 5 cut(s) 437, 502, 958, 1017, 1104
Alw26I GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2076, 2236, 2273
AlwI GGATC 1 cut(s) 2458
AlwNI CAGNNNCTG 3 cut(s) 989, 1685, 2276
Ama87I CYCGRG 1 cut(s) 1644
AoxI GGCC 7 cut(s) 39, 213, 428, 922, 1196, 1562, 2181
ApeKI GCWGC 6 cut(s) 19, 166, 227, 230, 529, 986
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
Asp700I GAANNNNTTC 2 cut(s) 1795, 2412
AspA2I CCTAGG 1 cut(s) 827
AspLEI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1579, 2449
AspS9I GGNCC 4 cut(s) 1682, 1834, 1936, 1942
AsuC2I CCSGG 2 cut(s) 878, 1025
AsuII TTCGAA 1 cut(s) 1799
AvaI CYCGRG 1 cut(s) 1644
AvaII GGWCC 4 cut(s) 1682, 1834, 1936, 1942
AvrII CCTAGG 1 cut(s) 827
AxyI CCTNAGG 1 cut(s) 1566
BaeGI GKGCMC 2 cut(s) 93, 2435
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 1104
BarI GAAGNNNNNNTAC 2 cut(s) 1273, 1305
BbrPI CACGTG 1 cut(s) 2000
BbsI GAAGAC 1 cut(s) 1054
Bbv12I GWGCWC 5 cut(s) 437, 502, 958, 1017, 1104
BbvI GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
BccI CCATC 7 cut(s) 325, 658, 762, 766, 1991, 2017, 2285
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 206, 240
BciT130I CCWGG 4 cut(s) 603, 1347, 1940, 2256
BclI TGATCA 3 cut(s) 658, 790, 1510
BcnI CCSGG 2 cut(s) 878, 1025
BcoDI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2076, 2236, 2273
BfaI CTAG 3 cut(s) 702, 828, 897
BfmI CTRYAG 3 cut(s) 1224, 2451, 2483
BfoI RGCGCY 3 cut(s) 241, 338, 2450
BglII AGATCT 1 cut(s) 575
BlnI CCTAGG 1 cut(s) 827
Bme1390I CCNGG 6 cut(s) 603, 878, 1025, 1347, 1940, 2256
Bme18I GGWCC 4 cut(s) 1682, 1834, 1936, 1942
BmeRI GACNNNNNGTC 1 cut(s) 2228
BmeT110I CYCGRG 1 cut(s) 1644
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 4 cut(s) 1682, 1834, 1936, 1942
BmiI GGNNCC 2 cut(s) 239, 1464
BmrFI CCNGG 6 cut(s) 603, 878, 1025, 1347, 1940, 2256
BmrI ACTGGG 1 cut(s) 1501
BmsI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 1969, 2053, 2095, 2360
BmuI ACTGGG 1 cut(s) 1501
BpiI GAAGAC 1 cut(s) 1054
BpmI CTGGAG 3 cut(s) 494, 1088, 2129
Bpu10I CCTNAGC 1 cut(s) 1617
Bpu14I TTCGAA 1 cut(s) 1799
BpuEI CTTGAG 2 cut(s) 400, 942
BpuMI CCSGG 2 cut(s) 878, 1025
Bsa29I ATCGAT 1 cut(s) 2352
BsaAI YACGTR 1 cut(s) 2000
BsaHI GRCGYC 2 cut(s) 238, 443
BsaI GGTCTC 1 cut(s) 2076
BsaJI CCNNGG 4 cut(s) 131, 602, 827, 1230
BsaXI ACNNNNNCTCC 8 cut(s) 377, 407, 1091, 1121, 1519, 1549, 1900, 1930
Bsc4I CCNNNNNNNGG 8 cut(s) 268, 306, 381, 725, 883, 1111, 1884, 1951
Bse1I ACTGG 7 cut(s) 70, 784, 1105, 1496, 1928, 2018, 2068
Bse21I CCTNAGG 1 cut(s) 1566
BseBI CCWGG 4 cut(s) 603, 1347, 1940, 2256
BseCI ATCGAT 1 cut(s) 2352
BseDI CCNNGG 4 cut(s) 131, 602, 827, 1230
BseLI CCNNNNNNNGG 8 cut(s) 268, 306, 381, 725, 883, 1111, 1884, 1951
BseNI ACTGG 7 cut(s) 70, 784, 1105, 1496, 1928, 2018, 2068
BseRI GAGGAG 4 cut(s) 193, 322, 361, 924
BseSI GKGCMC 2 cut(s) 93, 2435
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
BseYI CCCAGC 1 cut(s) 1341
BsgI GTGCAG 1 cut(s) 1952
Bsh1236I CGCG 7 cut(s) 133, 235, 250, 274, 504, 1232, 1579
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 7 cut(s) 41, 215, 430, 924, 1198, 1564, 2183
BshNI GGYRCC 1 cut(s) 237
BshVI ATCGAT 1 cut(s) 2352
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 5 cut(s) 437, 502, 958, 1017, 1104
BsiHKCI CYCGRG 1 cut(s) 1644
BsiSI CCGG 4 cut(s) 32, 877, 1024, 1919
BslFI GGGAC 3 cut(s) 379, 430, 658
BslI CCNNNNNNNGG 8 cut(s) 268, 306, 381, 725, 883, 1111, 1884, 1951
BsmAI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2076, 2236, 2273
BsmBI CGTCTC 3 cut(s) 175, 842, 1477
BsmFI GGGAC 3 cut(s) 379, 430, 658
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 7 cut(s) 41, 215, 430, 924, 1198, 1564, 2183
Bso31I GGTCTC 1 cut(s) 2076
BsoBI CYCGRG 1 cut(s) 1644
Bsp119I TTCGAA 1 cut(s) 1799
Bsp1286I GDGCHC 7 cut(s) 93, 437, 502, 958, 1017, 1104, 2435
Bsp1407I TGTACA 2 cut(s) 1755, 2384
BspANI GGCC 7 cut(s) 41, 215, 430, 924, 1198, 1564, 2183
BspDI ATCGAT 1 cut(s) 2352
BspFNI CGCG 7 cut(s) 133, 235, 250, 274, 504, 1232, 1579
BspLI GGNNCC 2 cut(s) 239, 1464
BspPI GGATC 1 cut(s) 2458
BspT104I TTCGAA 1 cut(s) 1799
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 2076
BsrBI CCGCTC 1 cut(s) 856
BsrGI TGTACA 2 cut(s) 1755, 2384
BsrI ACTGG 7 cut(s) 70, 784, 1105, 1496, 1928, 2018, 2068
BssECI CCNNGG 4 cut(s) 131, 602, 827, 1230
BssNI GRCGYC 2 cut(s) 238, 443
BssT1I CCWWGG 1 cut(s) 827
Bst2UI CCWGG 4 cut(s) 603, 1347, 1940, 2256
Bst4CI ACNGT 7 cut(s) 355, 421, 1267, 1331, 1753, 1760, 2484
Bst6I CTCTTC 1 cut(s) 993
BstACI GRCGYC 2 cut(s) 238, 443
BstAUI TGTACA 2 cut(s) 1755, 2384
BstBAI YACGTR 1 cut(s) 2000
BstBI TTCGAA 1 cut(s) 1799
BstC8I GCNNGC 7 cut(s) 49, 279, 339, 502, 2084, 2185, 2375
BstDSI CCRYGG 2 cut(s) 131, 1230
BstENI CCTNNNNNAGG 1 cut(s) 379
BstFNI CGCG 7 cut(s) 133, 235, 250, 274, 504, 1232, 1579
BstH2I RGCGCY 3 cut(s) 241, 338, 2450
BstHHI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1579, 2449
BstMAI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2076, 2236, 2273
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 8 cut(s) 28, 278, 280, 334, 463, 1342, 1583, 2073
BstNI CCWGG 4 cut(s) 603, 1347, 1940, 2256
BstNSI RCATGY 3 cut(s) 2086, 2167, 2391
BstSCI CCNGG 6 cut(s) 601, 876, 1023, 1345, 1938, 2254
BstSFI CTRYAG 3 cut(s) 1224, 2451, 2483
BstSLI GKGCMC 2 cut(s) 93, 2435
BstUI CGCG 7 cut(s) 133, 235, 250, 274, 504, 1232, 1579
BstV1I GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
BstV2I GAAGAC 1 cut(s) 1054
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 2352
Bsu36I CCTNAGG 1 cut(s) 1566
BsuRI GGCC 7 cut(s) 41, 215, 430, 924, 1198, 1564, 2183
BsuTUI ATCGAT 1 cut(s) 2352
BtgI CCRYGG 2 cut(s) 131, 1230
BtgZI GCGATG 1 cut(s) 1130
BtrI CACGTC 1 cut(s) 648
BtsIMutI CAGTG 3 cut(s) 1263, 1935, 2061
Cac8I GCNNGC 7 cut(s) 49, 279, 339, 502, 2084, 2185, 2375
CaiI CAGNNNCTG 3 cut(s) 989, 1685, 2276
CfoI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1579, 2449
Cfr13I GGNCC 4 cut(s) 1682, 1834, 1936, 1942
Cfr42I CCGCGG 2 cut(s) 134, 1233
ClaI ATCGAT 1 cut(s) 2352
CseI GACGC 1 cut(s) 432
Csp6I GTAC 4 cut(s) 761, 1623, 1756, 2385
CviQI GTAC 4 cut(s) 761, 1623, 1756, 2385
DinI GGCGCC 1 cut(s) 239
DrdI GACNNNNNNGTC 2 cut(s) 1409, 1680
DriI GACNNNNNGTC 1 cut(s) 2228
DseDI GACNNNNNNGTC 2 cut(s) 1409, 1680
EaeI YGGCCR 2 cut(s) 39, 213
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 993
Eam1105I GACNNNNNGTC 1 cut(s) 2228
EarI CTCTTC 1 cut(s) 993
Ecl136II GAGCTC 1 cut(s) 1102
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 827
Eco147I AGGCCT 2 cut(s) 1198, 2183
Eco24I GRGCYC 1 cut(s) 1104
Eco31I GGTCTC 1 cut(s) 2076
Eco47I GGWCC 4 cut(s) 1682, 1834, 1936, 1942
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 1102
Eco57I CTGAAG 3 cut(s) 1494, 1563, 1662
Eco72I CACGTG 1 cut(s) 2000
Eco81I CCTNAGG 1 cut(s) 1566
Eco88I CYCGRG 1 cut(s) 1644
EcoICRI GAGCTC 1 cut(s) 1102
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 1682
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 601, 1345, 1938, 2254
EcoT14I CCWWGG 1 cut(s) 827
EcoT22I ATGCAT 2 cut(s) 700, 2088
EcoT38I GRGCYC 1 cut(s) 1104
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 827
Esp3I CGTCTC 3 cut(s) 175, 842, 1477
FalI AAGNNNNNCTT 2 cut(s) 1204, 1236
FaqI GGGAC 3 cut(s) 379, 430, 658
FauI CCCGC 7 cut(s) 78, 334, 459, 497, 849, 1225, 2366
FauNDI CATATG 2 cut(s) 1852, 2379
FbaI TGATCA 3 cut(s) 658, 790, 1510
FriOI GRGCYC 1 cut(s) 1104
FspBI CTAG 3 cut(s) 702, 828, 897
GlaI GCGC 7 cut(s) 234, 239, 273, 282, 336, 1578, 2448
GsaI CCCAGC 1 cut(s) 1345
GsuI CTGGAG 3 cut(s) 494, 1088, 2129
HaeII RGCGCY 3 cut(s) 241, 338, 2450
HaeIII GGCC 7 cut(s) 41, 215, 430, 924, 1198, 1564, 2183
HapII CCGG 4 cut(s) 32, 877, 1024, 1919
HgaI GACGC 1 cut(s) 432
HhaI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1579, 2449
Hin1I GRCGYC 2 cut(s) 238, 443
Hin6I GCGC 7 cut(s) 233, 238, 272, 281, 335, 1577, 2447
HinP1I GCGC 7 cut(s) 233, 238, 272, 281, 335, 1577, 2447
HpaII CCGG 4 cut(s) 32, 877, 1024, 1919
Hpy166II GTNNAC 4 cut(s) 671, 1736, 1756, 2385
Hpy8I GTNNAC 4 cut(s) 671, 1736, 1756, 2385
Hpy99I CGWCG 3 cut(s) 406, 930, 1701
HpyAV CCTTC 5 cut(s) 385, 1230, 1526, 1686, 2004
HpyCH4III ACNGT 7 cut(s) 355, 421, 1267, 1331, 1753, 1760, 2484
HpyCH4IV ACGT 5 cut(s) 401, 647, 1486, 1999, 2212
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 278, 280, 334, 463, 1342, 1583, 2073
HpySE526I ACGT 5 cut(s) 401, 647, 1486, 1999, 2212
Hsp92I GRCGYC 2 cut(s) 238, 443
HspAI GCGC 7 cut(s) 233, 238, 272, 281, 335, 1577, 2447
KasI GGCGCC 1 cut(s) 237
Ksp22I TGATCA 3 cut(s) 658, 790, 1510
KspI CCGCGG 2 cut(s) 134, 1233
Lsp1109I GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
LweI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 1969, 2053, 2095, 2360
MaeI CTAG 3 cut(s) 702, 828, 897
MaeII ACGT 5 cut(s) 401, 647, 1486, 1999, 2212
MaeIII GTNAC 5 cut(s) 651, 940, 1361, 1487, 2345
MbiI CCGCTC 1 cut(s) 856
MfeI CAATTG 3 cut(s) 1247, 1382, 1467
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 7 cut(s) 93, 437, 502, 958, 1017, 1104, 2435
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 7 cut(s) 227, 329, 362, 395, 554, 989, 2136
MmeI TCCRAC 4 cut(s) 171, 303, 1534, 2157
Mph1103I ATGCAT 2 cut(s) 700, 2088
MroXI GAANNNNTTC 2 cut(s) 1795, 2412
MseI TTAA 3 cut(s) 1277, 1518, 2115
MslI CAYNNNNRTG 2 cut(s) 96, 2192
MspA1I CMGCKG 6 cut(s) 19, 133, 986, 1232, 1409, 1840
MspI CCGG 4 cut(s) 32, 877, 1024, 1919
MspR9I CCNGG 6 cut(s) 603, 878, 1025, 1347, 1940, 2256
MteI GCGCNGCGC 1 cut(s) 236
MunI CAATTG 3 cut(s) 1247, 1382, 1467
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 4 cut(s) 603, 1347, 1940, 2256
MvnI CGCG 7 cut(s) 133, 235, 250, 274, 504, 1232, 1579
MwoI GCNNNNNNNGC 8 cut(s) 28, 278, 280, 334, 463, 1342, 1583, 2073
NarI GGCGCC 1 cut(s) 238
NciI CCSGG 2 cut(s) 878, 1025
NdeI CATATG 2 cut(s) 1852, 2379
NlaIV GGNNCC 2 cut(s) 239, 1464
NmeAIII GCCGAG 3 cut(s) 241, 456, 2213
NmuCI GTSAC 4 cut(s) 651, 940, 1361, 2345
NsiI ATGCAT 2 cut(s) 700, 2088
NspI RCATGY 3 cut(s) 2086, 2167, 2391
NspV TTCGAA 1 cut(s) 1799
PaeI GCATGC 1 cut(s) 2086
PceI AGGCCT 2 cut(s) 1198, 2183
PciI ACATGT 1 cut(s) 2387
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 2 cut(s) 1795, 2412
PfeI GAWTC 8 cut(s) 35, 136, 313, 598, 870, 1719, 1801, 2354
PflFI GACNNNGTC 1 cut(s) 649
PflMI CCANNNNNTGG 2 cut(s) 1111, 1884
PfoI TCCNGGA 1 cut(s) 876
PleI GAGTC 7 cut(s) 226, 328, 362, 394, 553, 988, 2136
PluTI GGCGCC 1 cut(s) 241
PmaCI CACGTG 1 cut(s) 2000
PmlI CACGTG 1 cut(s) 2000
PpsI GAGTC 7 cut(s) 226, 328, 362, 394, 553, 988, 2136
Ppu21I YACGTR 1 cut(s) 2000
PpuMI RGGWCCY 1 cut(s) 1682
PscI ACATGT 1 cut(s) 2387
Psp124BI GAGCTC 1 cut(s) 1104
Psp1406I AACGTT 1 cut(s) 2212
Psp5II RGGWCCY 1 cut(s) 1682
Psp6I CCWGG 4 cut(s) 601, 1345, 1938, 2254
PspCI CACGTG 1 cut(s) 2000
PspFI CCCAGC 1 cut(s) 1341
PspGI CCWGG 4 cut(s) 601, 1345, 1938, 2254
PspN4I GGNNCC 2 cut(s) 239, 1464
PspPI GGNCC 4 cut(s) 1682, 1834, 1936, 1942
PspPPI RGGWCCY 1 cut(s) 1682
PstNI CAGNNNCTG 3 cut(s) 989, 1685, 2276
PsuI RGATCY 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 649
PvuII CAGCTG 3 cut(s) 986, 1409, 1840
RsaI GTAC 4 cut(s) 762, 1624, 1757, 2386
RsaNI GTAC 4 cut(s) 761, 1623, 1756, 2385
RseI CAYNNNNRTG 2 cut(s) 96, 2192
SacI GAGCTC 1 cut(s) 1104
SacII CCGCGG 2 cut(s) 134, 1233
SaqAI TTAA 3 cut(s) 1277, 1518, 2115
Sau96I GGNCC 4 cut(s) 1682, 1834, 1936, 1942
SchI GAGTC 7 cut(s) 227, 329, 362, 395, 554, 989, 2136
ScrFI CCNGG 6 cut(s) 603, 878, 1025, 1347, 1940, 2256
SduI GDGCHC 7 cut(s) 93, 437, 502, 958, 1017, 1104, 2435
SfaNI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 1969, 2053, 2095, 2360
SfcI CTRYAG 3 cut(s) 1224, 2451, 2483
SfoI GGCGCC 1 cut(s) 239
Sfr303I CCGCGG 2 cut(s) 134, 1233
SfuI TTCGAA 1 cut(s) 1799
SgrBI CCGCGG 2 cut(s) 134, 1233
SinI GGWCC 4 cut(s) 1682, 1834, 1936, 1942
SmiMI CAYNNNNRTG 2 cut(s) 96, 2192
SmlI CTYRAG 2 cut(s) 379, 957
SmoI CTYRAG 2 cut(s) 379, 957
SphI GCATGC 1 cut(s) 2086
SseBI AGGCCT 2 cut(s) 1198, 2183
SspDI GGCGCC 1 cut(s) 237
SspMI CTAG 3 cut(s) 702, 828, 897
SstI GAGCTC 1 cut(s) 1104
StuI AGGCCT 2 cut(s) 1198, 2183
StyD4I CCNGG 6 cut(s) 601, 876, 1023, 1345, 1938, 2254
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 7 cut(s) 355, 421, 1267, 1331, 1753, 1760, 2484
TaiI ACGT 5 cut(s) 404, 650, 1489, 2002, 2215
TaqI TCGA 9 cut(s) 60, 359, 366, 404, 1123, 1413, 1799, 2352, 2443
TaqII GACCGA 1 cut(s) 1959
TatI WGTACW 2 cut(s) 1755, 2384
TauI GCSGC 7 cut(s) 19, 238, 272, 277, 412, 1232, 2240
TfiI GAWTC 8 cut(s) 35, 136, 313, 598, 870, 1719, 1801, 2354
Tru1I TTAA 3 cut(s) 1277, 1518, 2115
Tru9I TTAA 3 cut(s) 1277, 1518, 2115
TscAI CASTG 3 cut(s) 1270, 1935, 2068
TseFI GTSAC 4 cut(s) 651, 940, 1361, 2345
TseI GCWGC 6 cut(s) 19, 166, 227, 230, 529, 986
Tsp45I GTSAC 4 cut(s) 651, 940, 1361, 2345
TspDTI ATGAA 7 cut(s) 70, 108, 114, 786, 1206, 1605, 1980
TspGWI ACGGA 4 cut(s) 20, 199, 866, 2456
TspRI CASTG 3 cut(s) 1270, 1935, 2068
Tth111I GACNNNGTC 1 cut(s) 649
Van91I CCANNNNNTGG 2 cut(s) 1111, 1884
VpaK11BI GGWCC 4 cut(s) 1682, 1834, 1936, 1942
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 1 cut(s) 77
XceI RCATGY 3 cut(s) 2086, 2167, 2391
XcmI CCANNNNNNNNNTGG 1 cut(s) 2037
XmaJI CCTAGG 1 cut(s) 827
XmnI GAANNNNTTC 2 cut(s) 1795, 2412
XspI CTAG 3 cut(s) 702, 828, 897
Zsp2I ATGCAT 2 cut(s) 700, 2088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.