Rw7G016070

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000302
Physical Location & Seq
Reverse (-)
16438102 .. 16440414
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016070.1

Sequence Viewer

Length: 2313 bp
ATGGAGAACAAAAAGTTTGTAGCCCTGCAGATCATATATCTTCTTGGCTTGAGTCTCATATTTTGCCTATCACTAGCTATTACCGATGGAGGCATGGGAATACTAAACCCGAAAGCTGAATCCGAGGCAGAAGAGGAAGATAGTGGCATGCAAACTTACATTGTTTGGGTGAAAAAACCGGTGAGGAGGCTATTTTCAGAATCTCATGAACATTTAGAGAGTTGGTACAAATCATTTTTGCCCAAAAGTTTTACAAACTCAAAGGAGGTGATGGACCAACGAATGGTTTACGCATACCACCATGTGGCTACTGGCTTTGCAGCAAAGCTTACAGCGGAGGAAGTAAAAGAAATGGAGACCAAGGATGGTTTTATATCAGCTCATCAAGATAGGATTCTCCCTCTGCACACTACCCACAGTCCGAACTTCTTGGGGTTGTACCAAGATTTGGGACTTTGGAAAGGGTCTAATTATGGTGAAGGAATCATTATTGGAGTTTTGGATAGTGGAATAACACCAGGTCATCCCTCATTCAGCGATGAAGGAGTGGGACCTCCTCCAGCTAAATGGAAAGGCAAGTGTGATTTCAATGGCACATTCTGCAACAACAAGCTTATTGGTGCCAGAAGTTTCCAGGGTGGTAAAACTACCGGAGCTCCACCAGTTGACCTTGACGGCCATGGAACCCACACGTCGAGCACAGCTGGAGGAAATTTTGTGAAAGGCGCCAACGTGTTTGGAATGGCAAAGGGAACAGCCTCTGGCATGGCACCTTATGCTCACTTGGCAATGTACCAAGTTTGTTCTGAACAGGGTTGTTCTGAAAGTGACATTGTAGCTGCCATGGACACTGCTATTGCCGATGGAGTGGACGTGCTCTCCCTCTCACTTGGCGGTGGCTCAGCTCCTTTCTATGCAGACGGAATCGCAGTCGGTGCATTTGGAGCAATTCAGAAGGGAATTTTTGTTAGCTGCTCAGCAGGAAATGATGGTCCTGCATATGGTTCTTTATCCAATGAAGCGCCATGGATTCTAACCGTCGGAGCAAGCACCATTGACAGAAGCATAAAGGCAACAGCAAAACTTGGCAATGGGCAGGAATATGATGGGGAATCGTTATTCCAGGATAAAAATTTTAGTTCAAATTTGTTACCTCTTGTTTATGCCGGTGCATATAGCAATGATTCAACAGCTCTGTGCGATGTAGGAACCCTTAATGGAGTTGAAGGGAAAGTGGTGCTGTGTGAAAGAGGTGGAGGAATTGGAAGAATTGCCAAAGGAGAAGAAGTCAAAAGAGCAGGTGGTGCTGCCATGATCCTCATGAACGAAGTCACTGATGGCTATAGCACCTTAGCTGATGCTCATGTGCTTCCTGCAACACATGTGAGTTATGCTGCAGGTTTGAACATCAAGGCCTACATAAACTCCACCTCAACACCTATGGCCACAATCTTGTTCAAAGGCACTGTCATCGGTGATCAAAATGCTCCTGCAGTCACTTCCTTTTCATCAAGAGGACCAAGCCTGGCAAGCACAGGAATCTTGAAACCTGATATCATTGGTCCCGGTGTTAGCATCTTAGCTGCATGGCCTGTTTCAGTGGACAATGGCACAGAATCTACGGCAACATTTAACATGGTTTCAGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGCAGCTTTGCTCAAGAGCTCACACCCCGACTGGTCACCAGCTGCCATCAAGTCTGCAATTGTGACAACCGCTGACATAGTAAACCTCGGAGGCAAACCTATTATTGATGAAACACTTGCTCCAGCAGACATCTTTGCCACTGGTGCAGGACATGTTAATCCTCCAAAAGCAGATGACCCCGGGCTAATCTACGACACAAAACCAACGGATTACATTCCCTACCTGTGCGGTTTGAACTACACAGACGATCAGATACAGATAATCACCCAACAAACAGTGAAGTGCTCTGAAGTAGGAGTCATACCTGAAGCGCAGCTAAATTATCCTTCATTTTCTATTATATTCCAGGACTTGTCTCAGTCTCAAGATTACACAAGGACGGTGACAAATGTTGGACCAGCTAATTCAACTTACGACTTGGATCTTAAGTTACCAGGTAAAATCGCCATGAGTGTGAGTCCTTCGCAGCTTAAGTTTACAGAAGTTGGCCAGACAATTACATACCTAGCAGAGTTTATCCCGGAAGATGGGGCAGGGAAGGATGCACCATTTTATCAGGGATATTTGAGATGGTTCTCTGATCAGCATTCTGTTACTAGCCCCATATCTGTGATCTTCGACACCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

770

Amino Acids

81.39

Weight (kDa)

5.01

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 52 - 136 2.8e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 159 - 584 5.2e-47 Subtilase family
PA PF02225 385 - 470 1.9e-13 PA domain
fn3_6 PF17766 665 - 764 7e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1289
Acc36I ACCTGC 2 cut(s) 1289, 1388
AccB1I GGYRCC 3 cut(s) 620, 725, 769
AccB7I CCANNNNNTGG 3 cut(s) 283, 304, 448
AciI CCGC 4 cut(s) 335, 894, 1749, 1908
AclWI GGATC 2 cut(s) 1309, 2109
AcoI YGGCCR 3 cut(s) 676, 1443, 2167
AcsI RAATTY 4 cut(s) 712, 960, 1132, 1144
AcuI CTGAAG 2 cut(s) 1989, 2007
AcyI GRCGYC 1 cut(s) 726
AdeI CACNNNGTG 1 cut(s) 304
AfaI GTAC 4 cut(s) 227, 440, 794, 1648
AfiI CCNNNNNNNGG 5 cut(s) 283, 304, 448, 1001, 2207
AflII CTTAAG 2 cut(s) 2105, 2150
AflIII ACRYGT 4 cut(s) 690, 732, 1381, 1831
AgeI ACCGGT 1 cut(s) 178
AjiI CACGTC 2 cut(s) 693, 874
AjnI CCWGG 6 cut(s) 517, 633, 1122, 1524, 2025, 2113
AleI CACNNNNGTG 1 cut(s) 1671
Alw21I GWGCWC 5 cut(s) 658, 701, 879, 1700, 1967
Alw26I GTCTC 4 cut(s) 59, 350, 2040, 2046
AlwI GGATC 2 cut(s) 1309, 2109
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 1859
AoxI GGCC 5 cut(s) 676, 1413, 1443, 1589, 2167
ApoI RAATTY 4 cut(s) 712, 960, 1132, 1144
ArsI GACNNNNNNTTYG 2 cut(s) 1452, 1484
AsiGI ACCGGT 1 cut(s) 178
AspLEI GCGC 3 cut(s) 728, 1024, 1993
AspS9I GGNCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
AsuC2I CCSGG 4 cut(s) 1566, 1860, 1861, 2201
AsuHPI GGTGA 9 cut(s) 181, 193, 280, 488, 1487, 1658, 1707, 1936, 2074
AvaI CYCGRG 1 cut(s) 1859
AvaII GGWCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
BalI TGGCCA 2 cut(s) 1445, 2169
BanI GGYRCC 3 cut(s) 620, 725, 769
BanII GRGCYC 2 cut(s) 658, 1700
Bbv12I GWGCWC 5 cut(s) 658, 701, 879, 1700, 1967
BceAI ACGGC 2 cut(s) 691, 1638
BcgI CGANNNNNNTGC 2 cut(s) 1453, 1487
BciT130I CCWGG 6 cut(s) 519, 635, 1124, 1526, 2027, 2115
BclI TGATCA 2 cut(s) 1477, 2260
BcnI CCSGG 4 cut(s) 1566, 1860, 1861, 2201
BcoDI GTCTC 4 cut(s) 59, 350, 2040, 2046
BfaI CTAG 3 cut(s) 74, 2186, 2277
BfmI CTRYAG 4 cut(s) 26, 1342, 1395, 1491
BfoI RGCGCY 2 cut(s) 729, 1025
BfrI CTTAAG 2 cut(s) 2105, 2150
BfuAI ACCTGC 2 cut(s) 1289, 1388
BlpI GCTNAGC 2 cut(s) 901, 976
Bme18I GGWCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
BmeT110I CYCGRG 1 cut(s) 1859
BmgBI CACGTC 2 cut(s) 693, 874
BmgT120I GGNCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
BmiI GGNNCC 7 cut(s) 552, 622, 685, 727, 771, 1210, 1564
BmsI GCATC 3 cut(s) 1348, 1584, 2212
BpmI CTGGAG 3 cut(s) 543, 726, 1785
Bpu10I CCTNAGC 1 cut(s) 1351
Bpu1102I GCTNAGC 2 cut(s) 901, 976
BpuEI CTTGAG 3 cut(s) 70, 1676, 2028
BpuMI CCSGG 4 cut(s) 1566, 1860, 1861, 2201
BsaHI GRCGYC 1 cut(s) 726
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 9 cut(s) 123, 360, 634, 679, 843, 1025, 1765, 1858, 1859
BsaWI WCCGGW 2 cut(s) 178, 650
Bsc4I CCNNNNNNNGG 5 cut(s) 283, 304, 448, 1001, 2207
Bse118I RCCGGY 2 cut(s) 178, 1166
Bse1I ACTGG 4 cut(s) 316, 662, 1715, 1825
Bse3DI GCAATG 4 cut(s) 795, 1096, 1186, 1677
BseBI CCWGG 6 cut(s) 519, 635, 1124, 1526, 2027, 2115
BseDI CCNNGG 9 cut(s) 123, 360, 634, 679, 843, 1025, 1765, 1858, 1859
BseGI GGATG 3 cut(s) 370, 523, 2227
BseLI CCNNNNNNNGG 5 cut(s) 283, 304, 448, 1001, 2207
BseMI GCAATG 4 cut(s) 795, 1096, 1186, 1677
BseMII CTCAG 4 cut(s) 915, 990, 1661, 2051
BseNI ACTGG 4 cut(s) 316, 662, 1715, 1825
BseRI GAGGAG 2 cut(s) 199, 546
BseYI CCCAGC 1 cut(s) 2304
BsgI GTGCAG 2 cut(s) 389, 1845
BshFI GGCC 5 cut(s) 678, 1415, 1445, 1591, 2169
BshNI GGYRCC 3 cut(s) 620, 725, 769
BshTI ACCGGT 1 cut(s) 178
BsiHKAI GWGCWC 5 cut(s) 658, 701, 879, 1700, 1967
BsiHKCI CYCGRG 1 cut(s) 1859
BsiSI CCGG 6 cut(s) 179, 651, 1167, 1566, 1860, 2201
BslFI GGGAC 3 cut(s) 465, 564, 1548
BslI CCNNNNNNNGG 5 cut(s) 283, 304, 448, 1001, 2207
BsmAI GTCTC 4 cut(s) 59, 350, 2040, 2046
BsmFI GGGAC 3 cut(s) 465, 564, 1548
BsmI GAATGC 1 cut(s) 2266
BsnI GGCC 5 cut(s) 678, 1415, 1445, 1591, 2169
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 1859
Bsp1286I GDGCHC 5 cut(s) 658, 701, 879, 1700, 1967
Bsp143I GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
Bsp1720I GCTNAGC 2 cut(s) 901, 976
Bsp19I CCATGG 3 cut(s) 679, 843, 1025
BspACI CCGC 4 cut(s) 335, 894, 1749, 1908
BspANI GGCC 5 cut(s) 678, 1415, 1445, 1591, 2169
BspCNI CTCAG 4 cut(s) 914, 989, 1662, 2050
BspHI TCATGA 2 cut(s) 205, 1320
BspLI GGNNCC 7 cut(s) 552, 622, 685, 727, 771, 1210, 1564
BspMAI CTGCAG 3 cut(s) 30, 1399, 1495
BspMI ACCTGC 2 cut(s) 1289, 1388
BspPI GGATC 2 cut(s) 1309, 2109
BspT107I GGYRCC 3 cut(s) 620, 725, 769
BspTI CTTAAG 2 cut(s) 2105, 2150
BspTNI GGTCTC 1 cut(s) 350
BsrDI GCAATG 4 cut(s) 795, 1096, 1186, 1677
BsrFI RCCGGY 2 cut(s) 178, 1166
BsrI ACTGG 4 cut(s) 316, 662, 1715, 1825
BssAI RCCGGY 2 cut(s) 178, 1166
BssECI CCNNGG 9 cut(s) 123, 360, 634, 679, 843, 1025, 1765, 1858, 1859
BssMI GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
BssNI GRCGYC 1 cut(s) 726
BssT1I CCWWGG 4 cut(s) 360, 679, 843, 1025
Bst2UI CCWGG 6 cut(s) 519, 635, 1124, 1526, 2027, 2115
Bst4CI ACNGT 5 cut(s) 419, 1039, 1468, 1957, 2062
Bst6I CTCTTC 1 cut(s) 126
BstACI GRCGYC 1 cut(s) 726
BstAFI CTTAAG 2 cut(s) 2105, 2150
BstAPI GCANNNNNTGC 4 cut(s) 600, 776, 935, 1304
BstC8I GCNNGC 3 cut(s) 149, 1048, 1531
BstDEI CTNAG 6 cut(s) 901, 976, 1351, 1579, 1670, 2037
BstDSI CCRYGG 3 cut(s) 679, 843, 1025
BstEII GGTNACC 1 cut(s) 1713
BstF5I GGATG 3 cut(s) 370, 523, 2227
BstH2I RGCGCY 2 cut(s) 729, 1025
BstHHI GCGC 3 cut(s) 728, 1024, 1993
BstKTI GATC 7 cut(s) 33, 1317, 1480, 1930, 2104, 2263, 2295
BstMAI GTCTC 4 cut(s) 59, 350, 2040, 2046
BstMBI GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
BstMWI GCNNNNNNNGC 8 cut(s) 600, 776, 785, 935, 944, 1304, 1530, 1823
BstNI CCWGG 6 cut(s) 519, 635, 1124, 1526, 2027, 2115
BstNSI RCATGY 3 cut(s) 151, 1385, 1835
BstPI GGTNACC 1 cut(s) 1713
BstSFI CTRYAG 4 cut(s) 26, 1342, 1395, 1491
BstX2I RGATCY 1 cut(s) 2101
BstXI CCANNNNNNTGG 1 cut(s) 567
BstYI RGATCY 1 cut(s) 2101
BsuRI GGCC 5 cut(s) 678, 1415, 1445, 1591, 2169
BtgI CCRYGG 3 cut(s) 679, 843, 1025
BtgZI GCGATG 2 cut(s) 552, 1215
BtrI CACGTC 2 cut(s) 693, 874
BtsCI GGATG 3 cut(s) 370, 523, 2227
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 6 cut(s) 849, 1332, 1464, 1605, 1818, 1962
BveI ACCTGC 2 cut(s) 1289, 1388
Cac8I GCNNGC 3 cut(s) 149, 1048, 1531
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 2 cut(s) 205, 1320
CfoI GCGC 3 cut(s) 728, 1024, 1993
Cfr10I RCCGGY 2 cut(s) 178, 1166
Cfr13I GGNCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
Cfr9I CCCGGG 1 cut(s) 1859
CsiI ACCWGGT 2 cut(s) 517, 2113
Csp6I GTAC 4 cut(s) 226, 439, 793, 1647
CspAI ACCGGT 1 cut(s) 178
CviQI GTAC 4 cut(s) 226, 439, 793, 1647
DdeI CTNAG 6 cut(s) 901, 976, 1351, 1579, 1670, 2037
DinI GGCGCC 1 cut(s) 727
DpnI GATC 7 cut(s) 32, 1316, 1479, 1929, 2103, 2262, 2294
DpnII GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
DraIII CACNNNGTG 1 cut(s) 304
EaeI YGGCCR 3 cut(s) 676, 1443, 2167
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Ecl136II GAGCTC 2 cut(s) 656, 1698
Eco130I CCWWGG 4 cut(s) 360, 679, 843, 1025
Eco147I AGGCCT 1 cut(s) 1415
Eco24I GRGCYC 2 cut(s) 658, 1700
Eco31I GGTCTC 1 cut(s) 350
Eco32I GATATC 1 cut(s) 1555
Eco47I GGWCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
Eco53kI GAGCTC 2 cut(s) 656, 1698
Eco57I CTGAAG 2 cut(s) 1989, 2007
Eco88I CYCGRG 1 cut(s) 1859
Eco91I GGTNACC 1 cut(s) 1713
EcoICRI GAGCTC 2 cut(s) 656, 1698
EcoO109I RGGNCCY 1 cut(s) 551
EcoO65I GGTNACC 1 cut(s) 1713
EcoRII CCWGG 6 cut(s) 517, 633, 1122, 1524, 2025, 2113
EcoRV GATATC 1 cut(s) 1555
EcoT14I CCWWGG 4 cut(s) 360, 679, 843, 1025
EcoT38I GRGCYC 2 cut(s) 658, 1700
EgeI GGCGCC 1 cut(s) 727
EheI GGCGCC 1 cut(s) 727
ErhI CCWWGG 4 cut(s) 360, 679, 843, 1025
FaqI GGGAC 3 cut(s) 465, 564, 1548
FauNDI CATATG 1 cut(s) 1000
FbaI TGATCA 2 cut(s) 1477, 2260
FokI GGATG 3 cut(s) 377, 510, 2234
FriOI GRGCYC 2 cut(s) 658, 1700
FspBI CTAG 3 cut(s) 74, 2186, 2277
GlaI GCGC 3 cut(s) 727, 1023, 1992
GsaI CCCAGC 1 cut(s) 2308
GsuI CTGGAG 3 cut(s) 543, 726, 1785
HaeII RGCGCY 2 cut(s) 729, 1025
HaeIII GGCC 5 cut(s) 678, 1415, 1445, 1591, 2169
HapII CCGG 6 cut(s) 179, 651, 1167, 1566, 1860, 2201
HhaI GCGC 3 cut(s) 728, 1024, 1993
Hin1I GRCGYC 1 cut(s) 726
Hin6I GCGC 3 cut(s) 726, 1022, 1991
HinP1I GCGC 3 cut(s) 726, 1022, 1991
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 2 cut(s) 326, 611
HpaII CCGG 6 cut(s) 179, 651, 1167, 1566, 1860, 2201
HphI GGTGA 9 cut(s) 181, 193, 280, 488, 1487, 1658, 1707, 1936, 2074
Hpy166II GTNNAC 6 cut(s) 289, 667, 871, 1603, 1762, 2157
Hpy188III TCNNGA 7 cut(s) 206, 386, 1321, 1512, 1543, 1693, 2045
Hpy8I GTNNAC 6 cut(s) 289, 667, 871, 1603, 1762, 2157
Hpy99I CGWCG 2 cut(s) 697, 1043
HpyAV CCTTC 7 cut(s) 473, 536, 949, 1220, 2016, 2151, 2212
HpyCH4III ACNGT 5 cut(s) 419, 1039, 1468, 1957, 2062
HpyCH4IV ACGT 3 cut(s) 692, 732, 873
HpyF10VI GCNNNNNNNGC 8 cut(s) 600, 776, 785, 935, 944, 1304, 1530, 1823
HpyF3I CTNAG 6 cut(s) 901, 976, 1351, 1579, 1670, 2037
HpySE526I ACGT 3 cut(s) 692, 732, 873
Hsp92I GRCGYC 1 cut(s) 726
HspAI GCGC 3 cut(s) 726, 1022, 1991
KasI GGCGCC 1 cut(s) 725
Ksp22I TGATCA 2 cut(s) 1477, 2260
Kzo9I GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
LmnI GCTCC 7 cut(s) 653, 661, 910, 944, 1043, 1492, 1804
LweI GCATC 3 cut(s) 1348, 1584, 2212
MabI ACCWGGT 2 cut(s) 517, 2113
MaeI CTAG 3 cut(s) 74, 2186, 2277
MaeII ACGT 3 cut(s) 692, 732, 873
MaeIII GTNAC 9 cut(s) 827, 1149, 1330, 1495, 1713, 1741, 2062, 2109, 2272
MalI GATC 7 cut(s) 32, 1316, 1479, 1929, 2103, 2262, 2294
MboI GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
MboII GAAGA 7 cut(s) 32, 143, 149, 1278, 1295, 2216, 2287
MfeI CAATTG 1 cut(s) 1737
MflI RGATCY 1 cut(s) 2101
MhlI GDGCHC 5 cut(s) 658, 701, 879, 1700, 1967
MlsI TGGCCA 2 cut(s) 1445, 2169
MluNI TGGCCA 2 cut(s) 1445, 2169
Mly113I GGCGCC 1 cut(s) 726
MlyI GAGTC 3 cut(s) 61, 1986, 2146
MmeI TCCRAC 2 cut(s) 1021, 2053
Mox20I TGGCCA 2 cut(s) 1445, 2169
MscI TGGCCA 2 cut(s) 1445, 2169
MseI TTAA 5 cut(s) 1215, 1632, 1836, 2106, 2151
MslI CAYNNNNRTG 3 cut(s) 1671, 2132, 2288
Msp20I TGGCCA 2 cut(s) 1445, 2169
MspA1I CMGCKG 4 cut(s) 335, 704, 1721, 1751
MspCI CTTAAG 2 cut(s) 2105, 2150
MspI CCGG 6 cut(s) 179, 651, 1167, 1566, 1860, 2201
MunI CAATTG 1 cut(s) 1737
Mva1269I GAATGC 1 cut(s) 2266
MvaI CCWGG 6 cut(s) 519, 635, 1124, 1526, 2027, 2115
MwoI GCNNNNNNNGC 8 cut(s) 600, 776, 785, 935, 944, 1304, 1530, 1823
NarI GGCGCC 1 cut(s) 726
NciI CCSGG 4 cut(s) 1566, 1860, 1861, 2201
NcoI CCATGG 3 cut(s) 679, 843, 1025
NdeI CATATG 1 cut(s) 1000
NdeII GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
NlaIV GGNNCC 7 cut(s) 552, 622, 685, 727, 771, 1210, 1564
NmuCI GTSAC 6 cut(s) 827, 1330, 1495, 1713, 1741, 2062
NspI RCATGY 3 cut(s) 151, 1385, 1835
OliI CACNNNNGTG 1 cut(s) 1671
PaeI GCATGC 1 cut(s) 151
PagI TCATGA 2 cut(s) 205, 1320
PaqCI CACCTGC 1 cut(s) 1289
PceI AGGCCT 1 cut(s) 1415
PciI ACATGT 2 cut(s) 1381, 1831
PctI GAATGC 1 cut(s) 2266
PflMI CCANNNNNTGG 3 cut(s) 283, 304, 448
PfoI TCCNGGA 3 cut(s) 1122, 2025, 2199
PinAI ACCGGT 1 cut(s) 178
PleI GAGTC 3 cut(s) 60, 1985, 2145
PluTI GGCGCC 1 cut(s) 729
PpsI GAGTC 3 cut(s) 60, 1985, 2145
PpuMI RGGWCCY 1 cut(s) 551
PscI ACATGT 2 cut(s) 1381, 1831
Psp124BI GAGCTC 2 cut(s) 658, 1700
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 6 cut(s) 517, 633, 1122, 1524, 2025, 2113
PspEI GGTNACC 1 cut(s) 1713
PspFI CCCAGC 1 cut(s) 2304
PspGI CCWGG 6 cut(s) 517, 633, 1122, 1524, 2025, 2113
PspN4I GGNNCC 7 cut(s) 552, 622, 685, 727, 771, 1210, 1564
PspPI GGNCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
PspPPI RGGWCCY 1 cut(s) 551
PstI CTGCAG 3 cut(s) 30, 1399, 1495
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 2101
PvuII CAGCTG 2 cut(s) 704, 1721
RsaI GTAC 4 cut(s) 227, 440, 794, 1648
RsaNI GTAC 4 cut(s) 226, 439, 793, 1647
RseI CAYNNNNRTG 3 cut(s) 1671, 2132, 2288
SacI GAGCTC 2 cut(s) 658, 1700
SaqAI TTAA 5 cut(s) 1215, 1632, 1836, 2106, 2151
Sau3AI GATC 7 cut(s) 30, 1314, 1477, 1927, 2101, 2260, 2292
Sau96I GGNCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
SchI GAGTC 3 cut(s) 61, 1986, 2146
SduI GDGCHC 5 cut(s) 658, 701, 879, 1700, 1967
SexAI ACCWGGT 2 cut(s) 517, 2113
SfaNI GCATC 3 cut(s) 1348, 1584, 2212
SfcI CTRYAG 4 cut(s) 26, 1342, 1395, 1491
SfoI GGCGCC 1 cut(s) 727
SinI GGWCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
SmaI CCCGGG 1 cut(s) 1861
SmiMI CAYNNNNRTG 3 cut(s) 1671, 2132, 2288
SmlI CTYRAG 5 cut(s) 49, 1691, 2043, 2105, 2150
SmoI CTYRAG 5 cut(s) 49, 1691, 2043, 2105, 2150
SphI GCATGC 1 cut(s) 151
SseBI AGGCCT 1 cut(s) 1415
SsiI CCGC 4 cut(s) 335, 894, 1749, 1908
SspDI GGCGCC 1 cut(s) 725
SspMI CTAG 3 cut(s) 74, 2186, 2277
SstI GAGCTC 2 cut(s) 658, 1700
StuI AGGCCT 1 cut(s) 1415
StyI CCWWGG 4 cut(s) 360, 679, 843, 1025
TaaI ACNGT 5 cut(s) 419, 1039, 1468, 1957, 2062
TaiI ACGT 3 cut(s) 695, 735, 876
TaqI TCGA 2 cut(s) 695, 2298
Tru1I TTAA 5 cut(s) 1215, 1632, 1836, 2106, 2151
Tru9I TTAA 5 cut(s) 1215, 1632, 1836, 2106, 2151
TscAI CASTG 6 cut(s) 856, 1339, 1471, 1605, 1825, 1962
TseFI GTSAC 6 cut(s) 827, 1330, 1495, 1713, 1741, 2062
Tsp45I GTSAC 6 cut(s) 827, 1330, 1495, 1713, 1741, 2062
TspDTI ATGAA 7 cut(s) 222, 555, 1032, 1337, 1497, 1803, 1998
TspGWI ACGGA 2 cut(s) 936, 1901
TspMI CCCGGG 1 cut(s) 1859
TspRI CASTG 6 cut(s) 856, 1339, 1471, 1605, 1825, 1962
Van91I CCANNNNNTGG 3 cut(s) 283, 304, 448
Vha464I CTTAAG 2 cut(s) 2105, 2150
VpaK11BI GGWCC 6 cut(s) 274, 551, 992, 1517, 1562, 2075
XapI RAATTY 4 cut(s) 712, 960, 1132, 1144
XceI RCATGY 3 cut(s) 151, 1385, 1835
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 1859
XspI CTAG 3 cut(s) 74, 2186, 2277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.