Prupe.5G085600_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9745365 .. 9746098
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085600.1

Sequence Viewer

Length: 711 bp
ATGGACTGGACACAATCTAATGCAACATTTAACATAATGTCGGGTACCTCAATGTCATGCCCTCACTTAAGTGGCATTGCAGGCTTGCTAAAGAGCTCACACGAGTCACACCCTGACTGGTCACCAGCTGCAATCAAATCTGCCATCATGACAAGCGCCAATGTACTGAACCTCGCAGGCATGCCCATTGTTGATCAAATGCTTAATCGCGCAGACGTATTGCAATTGGTGCAGGCCATGTTAACCCTTCGAAAGAAAATAACCCAGGAGGATTACATTCCTTATTTGTGTGGTTCAAATTACACAGACAAACAGATACAGATCATCACCCAAAAAACAGTGAACTGCTCTCAAGTTGGGGTCATACCAGAAGCACAACTAAACTATCCTTCATTTTCTATTACAACAGGATCCAATGAGACTGAATCTCAGTATTATACAAGGACAGTGAGGAACATTGGCCCGGCTAGTTCAACTTACAACTTGGATCTTCTTGTACCACATAAAATGGGCATGAGTGTGAAACCTGAGGTGCTTACATTCACAGAGGTCAACCAGGAGATTACATACCATGTGGAGTTTATTGCAGAAGATGGTGCTGGCAAGGATGGTGTAGCATTTGGTCAGGGGTATTTGAGATGGGTTTCTGATAAGCATAATGTTACTAGCCCAATATCTGTGATCTTTGACAATCATTCAATCTTAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

237

Amino Acids

26.01

Weight (kDa)

5.58

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 44
AccII CGCG 1 cut(s) 210
AclWI GGATC 3 cut(s) 405, 418, 495
AfaI GTAC 3 cut(s) 46, 165, 498
AflII CTTAAG 1 cut(s) 67
AgsI TTSAA 3 cut(s) 297, 474, 699
AjnI CCWGG 2 cut(s) 264, 555
AleI CACNNNNGTG 1 cut(s) 69
AluBI AGCT 2 cut(s) 96, 128
AluI AGCT 2 cut(s) 96, 128
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 3 cut(s) 405, 418, 495
AoxI GGCC 2 cut(s) 234, 460
ApeKI GCWGC 1 cut(s) 128
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 2 cut(s) 158, 212
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 114, 319
AsuII TTCGAA 1 cut(s) 250
AxyI CCTNAGG 1 cut(s) 528
BamHI GGATCC 1 cut(s) 410
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 98
BauI CACGAG 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 1 cut(s) 115
BccI CCATC 4 cut(s) 152, 587, 602, 633
BciT130I CCWGG 2 cut(s) 266, 557
BclI TGATCA 1 cut(s) 193
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 2 cut(s) 468, 666
BfoI RGCGCY 1 cut(s) 159
BfrI CTTAAG 1 cut(s) 67
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
Bme1390I CCNGG 3 cut(s) 266, 464, 557
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 46, 412
BmrFI CCNGG 3 cut(s) 266, 464, 557
Bpu14I TTCGAA 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 264
Bse1I ACTGG 2 cut(s) 11, 122
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 75
Bse8I GATNNNNATC 1 cut(s) 320
BseBI CCWGG 2 cut(s) 266, 557
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 1 cut(s) 613
BseJI GATNNNNATC 1 cut(s) 320
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 2 cut(s) 443, 519
BseNI ACTGG 2 cut(s) 11, 122
BseXI GCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 251
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 236, 462
BshNI GGYRCC 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 413
BsnI GGCC 2 cut(s) 236, 462
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 5 cut(s) 193, 321, 410, 487, 681
BspANI GGCC 2 cut(s) 236, 462
BspCNI CTCAG 2 cut(s) 442, 520
BspFNI CGCG 1 cut(s) 210
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 46, 412
BspPI GGATC 3 cut(s) 405, 418, 495
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 75
BsrI ACTGG 2 cut(s) 11, 122
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 5 cut(s) 193, 321, 410, 487, 681
BssSI CACGAG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 101
Bst2UI CCWGG 2 cut(s) 266, 557
Bst4CI ACNGT 2 cut(s) 340, 448
BstAFI CTTAAG 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 229
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 6 cut(s) 82, 86, 178, 182, 234, 601
BstDEI CTNAG 2 cut(s) 429, 528
BstEII GGTNACC 1 cut(s) 120
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 2 cut(s) 158, 212
BstKTI GATC 5 cut(s) 196, 324, 413, 490, 684
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 5 cut(s) 193, 321, 410, 487, 681
BstMWI GCNNNNNNNGC 2 cut(s) 81, 229
BstNI CCWGG 2 cut(s) 266, 557
BstNSI RCATGY 1 cut(s) 184
BstPI GGTNACC 1 cut(s) 120
BstSCI CCNGG 3 cut(s) 264, 462, 555
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 115
BstX2I RGATCY 2 cut(s) 410, 487
BstYI RGATCY 2 cut(s) 410, 487
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 2 cut(s) 236, 462
BtsCI GGATG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 345, 453
Cac8I GCNNGC 6 cut(s) 82, 86, 178, 182, 234, 601
CciI TCATGA 1 cut(s) 147
CfoI GCGC 2 cut(s) 158, 212
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 3 cut(s) 45, 164, 497
CviAII CATG 6 cut(s) 57, 148, 181, 238, 514, 572
CviJI RGCY 7 cut(s) 84, 96, 128, 236, 462, 467, 669
CviKI_1 RGCY 7 cut(s) 84, 96, 128, 236, 462, 467, 669
CviQI GTAC 3 cut(s) 45, 164, 497
DdeI CTNAG 2 cut(s) 429, 528
DpnI GATC 5 cut(s) 195, 323, 412, 489, 683
DpnII GATC 5 cut(s) 193, 321, 410, 487, 681
Ecl136II GAGCTC 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 96
EcoO65I GGTNACC 1 cut(s) 120
EcoRII CCWGG 2 cut(s) 264, 555
EcoT38I GRGCYC 1 cut(s) 98
FaeI CATG 6 cut(s) 60, 151, 184, 241, 517, 575
FatI CATG 6 cut(s) 56, 147, 180, 237, 513, 571
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 2 cut(s) 468, 666
GlaI GCGC 2 cut(s) 157, 211
GluI GCNGC 1 cut(s) 129
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 2 cut(s) 236, 462
HapII CCGG 1 cut(s) 464
HhaI GCGC 2 cut(s) 158, 212
Hin1II CATG 6 cut(s) 60, 151, 184, 241, 517, 575
Hin6I GCGC 2 cut(s) 156, 210
HinP1I GCGC 2 cut(s) 156, 210
HincII GTYRAC 2 cut(s) 243, 553
HindII GTYRAC 2 cut(s) 243, 553
HinfI GANTC 2 cut(s) 104, 425
HpaI GTTAAC 1 cut(s) 243
HpaII CCGG 1 cut(s) 464
HphI GGTGA 2 cut(s) 114, 319
Hpy166II GTNNAC 3 cut(s) 243, 343, 553
Hpy188I TCNGA 1 cut(s) 649
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 3 cut(s) 243, 343, 553
HpyAV CCTTC 2 cut(s) 257, 399
HpyCH4III ACNGT 2 cut(s) 340, 448
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 6 cut(s) 23, 80, 131, 223, 232, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 229
HpyF3I CTNAG 2 cut(s) 429, 528
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 6 cut(s) 60, 151, 184, 241, 517, 575
HspAI GCGC 2 cut(s) 156, 210
KpnI GGTACC 1 cut(s) 48
Ksp22I TGATCA 1 cut(s) 193
KspAI GTTAAC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 193, 321, 410, 487, 681
Lsp1109I GCAGC 1 cut(s) 115
MaeI CTAG 2 cut(s) 468, 666
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 3 cut(s) 105, 120, 661
MalI GATC 5 cut(s) 195, 323, 412, 489, 683
MboI GATC 5 cut(s) 193, 321, 410, 487, 681
MboII GAAGA 2 cut(s) 482, 602
MfeI CAATTG 1 cut(s) 224
MflI RGATCY 2 cut(s) 410, 487
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 2 cut(s) 224, 298
MlyI GAGTC 1 cut(s) 113
MnlI CCTC 7 cut(s) 58, 72, 182, 262, 444, 523, 541
MseI TTAA 5 cut(s) 30, 68, 204, 242, 704
MslI CAYNNNNRTG 2 cut(s) 69, 518
MspA1I CMGCKG 1 cut(s) 128
MspCI CTTAAG 1 cut(s) 67
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 3 cut(s) 266, 464, 557
MunI CAATTG 1 cut(s) 224
MvaI CCWGG 2 cut(s) 266, 557
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 2 cut(s) 81, 229
NciI CCSGG 1 cut(s) 464
NdeII GATC 5 cut(s) 193, 321, 410, 487, 681
NlaIII CATG 6 cut(s) 60, 151, 184, 241, 517, 575
NlaIV GGNNCC 2 cut(s) 46, 412
NmuCI GTSAC 2 cut(s) 105, 120
NspI RCATGY 1 cut(s) 184
NspV TTCGAA 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 69
PaeI GCATGC 1 cut(s) 184
PagI TCATGA 1 cut(s) 147
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 264, 555
PspEI GGTNACC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 264, 555
PspN4I GGNNCC 2 cut(s) 46, 412
PspPI GGNCC 1 cut(s) 461
PsuI RGATCY 2 cut(s) 410, 487
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 3 cut(s) 46, 165, 498
RsaNI GTAC 3 cut(s) 45, 164, 497
RseI CAYNNNNRTG 2 cut(s) 69, 518
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 5 cut(s) 30, 68, 204, 242, 704
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 5 cut(s) 193, 321, 410, 487, 681
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 3 cut(s) 266, 464, 557
SduI GDGCHC 1 cut(s) 98
SetI ASST 8 cut(s) 50, 98, 130, 174, 219, 529, 534, 552
SfuI TTCGAA 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 69, 518
SmlI CTYRAG 2 cut(s) 67, 351
SmoI CTYRAG 2 cut(s) 67, 351
SphI GCATGC 1 cut(s) 184
Sse9I AATT 2 cut(s) 224, 298
SspMI CTAG 2 cut(s) 468, 666
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 264, 462, 555
TaaI ACNGT 2 cut(s) 340, 448
TaiI ACGT 1 cut(s) 219
TaqI TCGA 1 cut(s) 250
TasI AATT 2 cut(s) 224, 298
TatI WGTACW 1 cut(s) 163
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 5 cut(s) 30, 68, 204, 242, 704
Tru9I TTAA 5 cut(s) 30, 68, 204, 242, 704
TscAI CASTG 2 cut(s) 345, 453
TseFI GTSAC 2 cut(s) 105, 120
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 2 cut(s) 105, 120
TspDTI ATGAA 1 cut(s) 381
TspRI CASTG 2 cut(s) 345, 453
Vha464I CTTAAG 1 cut(s) 67
XceI RCATGY 1 cut(s) 184
XspI CTAG 2 cut(s) 468, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.