Rroxscaffold_3G00256280

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50654096 .. 50656354
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256280.1

Sequence Viewer

Length: 2259 bp
ATGGCTGTGATTCCATTTCTTTTTCTTGTTTTCATGCTTAGCTTCTCTCATGGGATTGCTCAGGAAACCGAGCTTTCTCCTGTCCCTACGAAAACGAGCAATTTACAGACATACATTGTCCATGTAATGCAACCAGAGGGTAAAGTTTTTGCCCAAACAGAAGACCTGGCGAGCTGGCACAAATCTTTTCTGCCATCTATGACAGCAAGCTCTGATGAGCAAACACGCTTGCTTTACTCCTACAAAACTGTGATCAGTGGTTTTTCAGCAAGGCTAACTCAAGAGGAAGTGAAAGCGATGGAACAGATGAATGGTTTTGTGGCAGCACATCCTGAACGAGTGTTTCGTCGGAAAACCACACACACTCCCAACTTCTTGGGGCTGAACAAACAAGCGGGAATTTGGAAAGACTCAAACTTTGGGAAGGGAGTGATCATTGGAGTACTAGATGGCGGAGTATTCCCCAGCCACCCTTCATTTAGTGGTGCAGGAATTCCACCTCCACCTGCTAAATGGAAAGGGAGGTGTGACTTCAATGTGTCAGAATGCAATAATAAATTAATTGGAGCACAGTCGTTCAATCTTGCGGCCATGGCATTGAAGGGAGCAAAAGCTGAACCGCCAACTGATGAAGACGGACATGGAACACACACAGCAAGCACAGCTGGTGGAGCATTTGTGCAGAATGCTGATGCGCTAGGAAATGCCAAAGGCACAGCAGTTGGGATGGCACCTTATGCTCACCTGGCAATATACAAAGTGTGTTTTGGAGAACCCTGTCCAGAAAGTGATATCCTAGCTGCCCTTGAAGCTGCTGTTCACGATGGTGTTGATGTGATCTCAATCTCCCTTGGAGAAGATCCAGTTCCATTTTTCCAGGACAGTACTGCAATTGGTTCATTTGCAGCAATGCAAAAGGGAATATTTGTTAGTTGTTCAGCCGGAAATTCTGGTCCATTTAACGGCACATTATCTAATGAAGCCCCTTGGATTCTCACAGTTGGAGCAAGCACCCTCGATAGACGCATTGTAGCCTCAGCCAAGCTTGGAAATGGATTAGTATTTGACGGTGAATCTCTCTTTCAACCGAAAGATTTCCCTTCAACATTATTGCCTCTTGTTTATGCTGGAGTAAATGGCAAAGCAGAGTCTGCATTTTGCGCTGAAGGATCCTTAAAAAACATAAGTGTGAAAGGTAAGGTGGTTGTGTGTGAGAGAGGTGGGGGAATAGGAAGAATTGACAAGGGAGTGGAAGTGAAAAATGCAGGGGGTGCGGCCATGATTCTACTGAATGCGGAAACTGATGGATTCAGTACCACAGCTGATGCTCATGTTCTTCCTGCAGCACATGTGACCCATGCAGCTGGACTGAAGATCAAAGCATACATAAATTCGACGGCAACACCAACAGCGACAATCTTATTCAAAGGAACTGTCATCGGAGACTCAACATCTCCAGCTGTTGCTTCCTTCTCTTCAAGAGGCCCCAGCCAGGCAAGTCCAGGAATTCTGAAACCAGACATAATTGGACCAGGTGTAAGCATTCTTGCTGCATGGCCATTCCCTCTTGACAACAACACAAAATCAACATCCACTTTTAATATAATTTCCGGAACATCAATGTCATGTCCACATCTCAGTGGAATTGCAGCTCTGCTCAAGAGCTCTCACCCATACTGGTCACCAGCTGCCATTAAATCTGCTATAATGACTTCTGCTGATCTTCTAAACTTGCAAGGCAAACGGATTTTTGATGAAACACTTCAGACTGCAGATATCTTAGCCACCGGTGCGGGCCATGTCAACCCATCAAAGGCGACCGACCCAGGACTGATATATGATATTCAACCTGATGAGTATATTCCATATCTTTGTGGCTTGGGCTACAACGATACTGGAGTCAGTATCCTCGTGCACAGACCAATAAAGTGCTCAAAGGTATCAAGCATCCCTGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCAAGCTGGGACCATCTCAGACATTCACAAGAACTGTGACAAATGTTGGTGCCCCATATTCCACTTATTCGGTCAAGGTATATGCACCTCTAGGAACCTATGTGACTATCAAACCCAGCACGCTTTACTTTACAGAGGTGAACCAGAAGGCGTCATATTCAGTGACATTCAGTCGTACTTCAGGAGGTAAAGCTGGTGCATTCACACAGGGATTTATAACATGGGCTTCTATTAAACACGTTGTCCGGAGTCCAGTTTCTGTTTTATTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

752

Amino Acids

79.21

Weight (kDa)

7.61

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 115 1.3e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 575 2e-44 Subtilase family
PA PF02225 372 - 459 8.7e-13 PA domain
fn3_6 PF17766 654 - 749 1.2e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2201
AarI CACCTGC 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 514
AccB1I GGYRCC 2 cut(s) 730, 2033
AccIII TCCGGA 2 cut(s) 1610, 2229
AciI CCGC 7 cut(s) 395, 453, 587, 620, 1274, 1295, 1793
AclWI GGATC 3 cut(s) 854, 1164, 1177
AcoI YGGCCR 3 cut(s) 588, 1275, 1556
AcsI RAATTY 5 cut(s) 399, 492, 946, 1390, 1506
AcuI CTGAAG 5 cut(s) 1185, 1391, 1748, 1974, 2148
AcyI GRCGYC 1 cut(s) 2135
AfaI GTAC 4 cut(s) 444, 886, 1315, 2161
AfiI CCNNNNNNNGG 3 cut(s) 962, 1492, 1813
AflIII ACRYGT 2 cut(s) 1348, 2221
AgeI ACCGGT 1 cut(s) 1787
AgsI TTSAA 9 cut(s) 535, 580, 601, 809, 1085, 1104, 1426, 1479, 1847
AhdI GACNNNNNGTC 1 cut(s) 2154
AjnI CCWGG 7 cut(s) 165, 744, 876, 1491, 1501, 1531, 1825
AleI CACNNNNGTG 1 cut(s) 825
Alw21I GWGCWC 4 cut(s) 571, 1667, 1917, 1934
Alw26I GTCTC 1 cut(s) 1437
Alw44I GTGCAC 1 cut(s) 1913
AlwI GGATC 3 cut(s) 854, 1164, 1177
AlwNI CAGNNNCTG 2 cut(s) 1151, 2243
Aor13HI TCCGGA 2 cut(s) 1610, 2229
AoxI GGCC 5 cut(s) 588, 1275, 1483, 1556, 1795
ApaLI GTGCAC 1 cut(s) 1913
ApeKI GCWGC 9 cut(s) 323, 800, 812, 905, 1343, 1361, 1550, 1649, 1688
ApoI RAATTY 5 cut(s) 399, 492, 946, 1390, 1506
ArsI GACNNNNNNTTYG 4 cut(s) 401, 433, 1419, 1451
AseI ATTAAT 1 cut(s) 560
AsiGI ACCGGT 1 cut(s) 1787
Asp700I GAANNNNTTC 2 cut(s) 1094, 1761
AspLEI GCGC 2 cut(s) 697, 1163
AspS9I GGNCC 5 cut(s) 953, 1484, 1529, 1795, 1994
AsuHPI GGTGA 5 cut(s) 734, 1082, 1661, 1674, 2134
AvaII GGWCC 3 cut(s) 953, 1529, 1994
BaeGI GKGCMC 2 cut(s) 1917, 2038
BaeI ACNNNNGTAYC 2 cut(s) 1884, 1917
BalI TGGCCA 1 cut(s) 1558
BamHI GGATCC 1 cut(s) 1169
BanI GGYRCC 2 cut(s) 730, 2033
BanII GRGCYC 1 cut(s) 1667
BauI CACGAG 1 cut(s) 1910
BbsI GAAGAC 2 cut(s) 168, 639
Bbv12I GWGCWC 4 cut(s) 571, 1667, 1917, 1934
BbvCI CCTCAGC 1 cut(s) 1036
BbvI GCAGC 9 cut(s) 335, 787, 799, 917, 1355, 1373, 1537, 1661, 1675
BccI CCATC 8 cut(s) 202, 292, 443, 721, 818, 1298, 1816, 2005
BceAI ACGGC 2 cut(s) 979, 1413
BciT130I CCWGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
BciVI GTATCC 1 cut(s) 1916
BclI TGATCA 2 cut(s) 252, 432
BcoDI GTCTC 1 cut(s) 1437
BfaI CTAG 4 cut(s) 446, 698, 797, 2075
BfmI CTRYAG 2 cut(s) 1341, 1770
BfuAI ACCTGC 1 cut(s) 514
BfuI GTATCC 1 cut(s) 1916
BlpI GCTNAGC 1 cut(s) 38
BmcAI AGTACT 2 cut(s) 444, 886
Bme1390I CCNGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
Bme18I GGWCC 3 cut(s) 953, 1529, 1994
BmeRI GACNNNNNGTC 1 cut(s) 2154
BmgT120I GGNCC 5 cut(s) 953, 1484, 1529, 1795, 1994
BmiI GGNNCC 6 cut(s) 732, 1171, 1486, 1995, 2035, 2080
BmrFI CCNGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
BmsI GCATC 3 cut(s) 682, 1315, 1956
BpiI GAAGAC 2 cut(s) 168, 639
BpmI CTGGAG 3 cut(s) 1149, 1440, 1917
Bpu10I CCTNAGC 2 cut(s) 60, 1036
Bpu1102I GCTNAGC 1 cut(s) 38
BpuEI CTTGAG 2 cut(s) 264, 1643
BsaBI GATNNNNATC 1 cut(s) 842
BsaHI GRCGYC 1 cut(s) 2135
BsaJI CCNNGG 4 cut(s) 591, 850, 986, 1825
BsaWI WCCGGW 3 cut(s) 1610, 1787, 2229
BsaXI ACNNNNNCTCC 6 cut(s) 349, 379, 663, 693, 1239, 1269
Bsc4I CCNNNNNNNGG 3 cut(s) 962, 1492, 1813
Bse118I RCCGGY 1 cut(s) 1787
Bse1I ACTGG 4 cut(s) 863, 1682, 1900, 2237
Bse3DI GCAATG 1 cut(s) 915
Bse8I GATNNNNATC 1 cut(s) 842
BseAI TCCGGA 2 cut(s) 1610, 2229
BseBI CCWGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
BseDI CCNNGG 4 cut(s) 591, 850, 986, 1825
BseGI GGATG 4 cut(s) 328, 732, 1589, 1947
BseJI GATNNNNATC 1 cut(s) 842
BseLI CCNNNNNNNGG 3 cut(s) 962, 1492, 1813
BseMI GCAATG 1 cut(s) 915
BseMII CTCAG 4 cut(s) 74, 1050, 1651, 2015
BseNI ACTGG 4 cut(s) 863, 1682, 1900, 2237
BseSI GKGCMC 2 cut(s) 1917, 2038
BseXI GCAGC 9 cut(s) 335, 787, 799, 917, 1355, 1373, 1537, 1661, 1675
BseYI CCCAGC 4 cut(s) 464, 1487, 1990, 2099
BsgI GTGCAG 2 cut(s) 507, 701
Bsh1285I CGRYCG 1 cut(s) 1821
BshFI GGCC 5 cut(s) 590, 1277, 1485, 1558, 1797
BshNI GGYRCC 2 cut(s) 730, 2033
BshTI ACCGGT 1 cut(s) 1787
BsiEI CGRYCG 1 cut(s) 1821
BsiHKAI GWGCWC 4 cut(s) 571, 1667, 1917, 1934
BsiSI CCGG 4 cut(s) 942, 1611, 1788, 2230
BslFI GGGAC 2 cut(s) 68, 2007
BslI CCNNNNNNNGG 3 cut(s) 962, 1492, 1813
BsmAI GTCTC 1 cut(s) 1437
BsmFI GGGAC 2 cut(s) 68, 2007
BsmI GAATGC 5 cut(s) 551, 691, 1297, 1542, 2183
BsnI GGCC 5 cut(s) 590, 1277, 1485, 1558, 1797
Bsp1286I GDGCHC 5 cut(s) 571, 1667, 1917, 1934, 2038
Bsp13I TCCGGA 2 cut(s) 1610, 2229
Bsp143I GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
Bsp1720I GCTNAGC 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 7 cut(s) 395, 453, 587, 620, 1274, 1295, 1793
BspANI GGCC 5 cut(s) 590, 1277, 1485, 1558, 1797
BspCNI CTCAG 4 cut(s) 73, 1049, 1650, 2014
BspEI TCCGGA 2 cut(s) 1610, 2229
BspLI GGNNCC 6 cut(s) 732, 1171, 1486, 1995, 2035, 2080
BspMAI CTGCAG 2 cut(s) 1345, 1774
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 3 cut(s) 854, 1164, 1177
BspT107I GGYRCC 2 cut(s) 730, 2033
BsrDI GCAATG 1 cut(s) 915
BsrFI RCCGGY 1 cut(s) 1787
BsrI ACTGG 4 cut(s) 863, 1682, 1900, 2237
BssAI RCCGGY 1 cut(s) 1787
BssECI CCNNGG 4 cut(s) 591, 850, 986, 1825
BssMI GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
BssNI GRCGYC 1 cut(s) 2135
BssSI CACGAG 1 cut(s) 1910
BssT1I CCWWGG 3 cut(s) 591, 850, 986
Bst2BI CACGAG 1 cut(s) 1910
Bst2UI CCWGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
Bst4CI ACNGT 7 cut(s) 250, 573, 884, 1000, 1070, 1435, 2020
Bst6I CTCTTC 1 cut(s) 1480
BstACI GRCGYC 1 cut(s) 2135
BstAPI GCANNNNNTGC 3 cut(s) 737, 1151, 1271
BstC8I GCNNGC 8 cut(s) 172, 176, 208, 230, 658, 1009, 1795, 2105
BstDEI CTNAG 6 cut(s) 38, 60, 1036, 1637, 1780, 2001
BstDSI CCRYGG 1 cut(s) 591
BstEII GGTNACC 1 cut(s) 1680
BstF5I GGATG 4 cut(s) 328, 732, 1589, 1947
BstHHI GCGC 2 cut(s) 697, 1163
BstKTI GATC 7 cut(s) 255, 435, 840, 862, 1172, 1377, 1723
BstMAI GTCTC 1 cut(s) 1437
BstMBI GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
BstMCI CGRYCG 1 cut(s) 1821
BstNI CCWGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
BstNSI RCATGY 1 cut(s) 1352
BstPI GGTNACC 1 cut(s) 1680
BstSCI CCNGG 7 cut(s) 165, 744, 876, 1491, 1501, 1531, 1825
BstSFI CTRYAG 2 cut(s) 1341, 1770
BstSLI GKGCMC 2 cut(s) 1917, 2038
BstV1I GCAGC 9 cut(s) 335, 787, 799, 917, 1355, 1373, 1537, 1661, 1675
BstV2I GAAGAC 2 cut(s) 168, 639
BstX2I RGATCY 2 cut(s) 859, 1169
BstXI CCANNNNNNTGG 2 cut(s) 376, 1364
BstYI RGATCY 2 cut(s) 859, 1169
BsuI GTATCC 1 cut(s) 1916
BsuRI GGCC 5 cut(s) 590, 1277, 1485, 1558, 1797
BtgI CCRYGG 1 cut(s) 591
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 4 cut(s) 328, 732, 1589, 1947
BtsIMutI CAGTG 3 cut(s) 262, 1645, 2151
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 8 cut(s) 172, 176, 208, 230, 658, 1009, 1795, 2105
CaiI CAGNNNCTG 2 cut(s) 1151, 2243
CfoI GCGC 2 cut(s) 697, 1163
Cfr10I RCCGGY 1 cut(s) 1787
Cfr13I GGNCC 5 cut(s) 953, 1484, 1529, 1795, 1994
CseI GACGC 2 cut(s) 1032, 2124
CsiI ACCWGGT 1 cut(s) 1531
Csp6I GTAC 4 cut(s) 443, 885, 1314, 2160
CspAI ACCGGT 1 cut(s) 1787
CviQI GTAC 4 cut(s) 443, 885, 1314, 2160
DdeI CTNAG 6 cut(s) 38, 60, 1036, 1637, 1780, 2001
DpnI GATC 7 cut(s) 254, 434, 839, 861, 1171, 1376, 1722
DpnII GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
DriI GACNNNNNGTC 1 cut(s) 2154
EaeI YGGCCR 3 cut(s) 588, 1275, 1556
Eam1104I CTCTTC 1 cut(s) 1480
Eam1105I GACNNNNNGTC 1 cut(s) 2154
EarI CTCTTC 1 cut(s) 1480
EciI GGCGGA 1 cut(s) 468
Ecl136II GAGCTC 1 cut(s) 1665
Eco130I CCWWGG 3 cut(s) 591, 850, 986
Eco24I GRGCYC 1 cut(s) 1667
Eco32I GATATC 2 cut(s) 793, 1777
Eco47I GGWCC 3 cut(s) 953, 1529, 1994
Eco53kI GAGCTC 1 cut(s) 1665
Eco57I CTGAAG 5 cut(s) 1185, 1391, 1748, 1974, 2148
Eco91I GGTNACC 1 cut(s) 1680
EcoICRI GAGCTC 1 cut(s) 1665
EcoO109I RGGNCCY 1 cut(s) 1484
EcoO65I GGTNACC 1 cut(s) 1680
EcoRI GAATTC 2 cut(s) 492, 1506
EcoRII CCWGG 7 cut(s) 165, 744, 876, 1491, 1501, 1531, 1825
EcoRV GATATC 2 cut(s) 793, 1777
EcoT14I CCWWGG 3 cut(s) 591, 850, 986
EcoT38I GRGCYC 1 cut(s) 1667
ErhI CCWWGG 3 cut(s) 591, 850, 986
FaqI GGGAC 2 cut(s) 68, 2007
FauI CCCGC 2 cut(s) 388, 1786
FbaI TGATCA 2 cut(s) 252, 432
FokI GGATG 4 cut(s) 315, 739, 1576, 1934
FriOI GRGCYC 1 cut(s) 1667
FspBI CTAG 4 cut(s) 446, 698, 797, 2075
GlaI GCGC 2 cut(s) 696, 1162
GsaI CCCAGC 4 cut(s) 468, 1491, 1994, 2103
GsuI CTGGAG 3 cut(s) 1149, 1440, 1917
HaeIII GGCC 5 cut(s) 590, 1277, 1485, 1558, 1797
HapII CCGG 4 cut(s) 942, 1611, 1788, 2230
HgaI GACGC 2 cut(s) 1032, 2124
HhaI GCGC 2 cut(s) 697, 1163
Hin1I GRCGYC 1 cut(s) 2135
Hin6I GCGC 2 cut(s) 695, 1161
HinP1I GCGC 2 cut(s) 695, 1161
HincII GTYRAC 1 cut(s) 1804
HindII GTYRAC 1 cut(s) 1804
HindIII AAGCTT 1 cut(s) 1043
HpaII CCGG 4 cut(s) 942, 1611, 1788, 2230
HphI GGTGA 5 cut(s) 734, 1082, 1661, 1674, 2134
Hpy166II GTNNAC 5 cut(s) 820, 1631, 1804, 1915, 2125
Hpy188I TCNGA 7 cut(s) 214, 351, 544, 1442, 1512, 1767, 2004
Hpy8I GTNNAC 5 cut(s) 820, 1631, 1804, 1915, 2125
Hpy99I CGWCG 2 cut(s) 351, 1399
HpyAV CCTTC 9 cut(s) 418, 483, 595, 1110, 1160, 1480, 1949, 1983, 2125
HpyCH4III ACNGT 7 cut(s) 250, 573, 884, 1000, 1070, 1435, 2020
HpyCH4IV ACGT 1 cut(s) 2223
HpyF3I CTNAG 6 cut(s) 38, 60, 1036, 1637, 1780, 2001
HpySE526I ACGT 1 cut(s) 2223
Hsp92I GRCGYC 1 cut(s) 2135
HspAI GCGC 2 cut(s) 695, 1161
Kpn2I TCCGGA 2 cut(s) 1610, 2229
Ksp22I TGATCA 2 cut(s) 252, 432
Kzo9I GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
LmnI GCTCC 4 cut(s) 566, 605, 671, 1004
Lsp1109I GCAGC 9 cut(s) 335, 787, 799, 917, 1355, 1373, 1537, 1661, 1675
LweI GCATC 3 cut(s) 682, 1315, 1956
MabI ACCWGGT 1 cut(s) 1531
MaeI CTAG 4 cut(s) 446, 698, 797, 2075
MaeII ACGT 1 cut(s) 2223
MaeIII GTNAC 6 cut(s) 527, 1351, 1680, 2020, 2086, 2146
MalI GATC 7 cut(s) 254, 434, 839, 861, 1171, 1376, 1722
MboI GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
MboII GAAGA 8 cut(s) 173, 644, 869, 1245, 1328, 1384, 1467, 1715
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 2 cut(s) 859, 1169
MhlI GDGCHC 5 cut(s) 571, 1667, 1917, 1934, 2038
MlsI TGGCCA 1 cut(s) 1558
MluNI TGGCCA 1 cut(s) 1558
MlyI GAGTC 5 cut(s) 404, 1157, 1439, 1908, 2242
MmeI TCCRAC 2 cut(s) 329, 982
Mox20I TGGCCA 1 cut(s) 1558
MroI TCCGGA 2 cut(s) 1610, 2229
MroXI GAANNNNTTC 2 cut(s) 1094, 1761
MscI TGGCCA 1 cut(s) 1558
MseI TTAA 6 cut(s) 560, 960, 1175, 1599, 1695, 2217
MslI CAYNNNNRTG 3 cut(s) 825, 1187, 1638
Msp20I TGGCCA 1 cut(s) 1558
MspA1I CMGCKG 5 cut(s) 665, 1322, 1364, 1460, 1688
MspI CCGG 4 cut(s) 942, 1611, 1788, 2230
MspR9I CCNGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 5 cut(s) 551, 691, 1297, 1542, 2183
MvaI CCWGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
NcoI CCATGG 1 cut(s) 591
NdeII GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
NlaIV GGNNCC 6 cut(s) 732, 1171, 1486, 1995, 2035, 2080
NmuCI GTSAC 6 cut(s) 527, 1351, 1680, 2020, 2086, 2146
NspI RCATGY 1 cut(s) 1352
OliI CACNNNNGTG 1 cut(s) 825
PaqCI CACCTGC 1 cut(s) 514
PciI ACATGT 1 cut(s) 1348
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 5 cut(s) 551, 691, 1297, 1542, 2183
PdmI GAANNNNTTC 2 cut(s) 1094, 1761
PfeI GAWTC 5 cut(s) 10, 991, 1073, 1282, 1308
PfoI TCCNGGA 2 cut(s) 876, 1501
PinAI ACCGGT 1 cut(s) 1787
PleI GAGTC 5 cut(s) 404, 1156, 1439, 1907, 2241
PpsI GAGTC 5 cut(s) 404, 1156, 1439, 1907, 2241
PscI ACATGT 1 cut(s) 1348
PshBI ATTAAT 1 cut(s) 560
PsiI TTATAA 1 cut(s) 2201
Psp124BI GAGCTC 1 cut(s) 1667
Psp6I CCWGG 7 cut(s) 165, 744, 876, 1491, 1501, 1531, 1825
PspEI GGTNACC 1 cut(s) 1680
PspFI CCCAGC 4 cut(s) 464, 1487, 1990, 2099
PspGI CCWGG 7 cut(s) 165, 744, 876, 1491, 1501, 1531, 1825
PspN4I GGNNCC 6 cut(s) 732, 1171, 1486, 1995, 2035, 2080
PspPI GGNCC 5 cut(s) 953, 1484, 1529, 1795, 1994
PstI CTGCAG 2 cut(s) 1345, 1774
PstNI CAGNNNCTG 2 cut(s) 1151, 2243
PsuI RGATCY 2 cut(s) 859, 1169
PvuII CAGCTG 5 cut(s) 665, 1322, 1364, 1460, 1688
RsaI GTAC 4 cut(s) 444, 886, 1315, 2161
RsaNI GTAC 4 cut(s) 443, 885, 1314, 2160
RseI CAYNNNNRTG 3 cut(s) 825, 1187, 1638
SacI GAGCTC 1 cut(s) 1667
SaqAI TTAA 6 cut(s) 560, 960, 1175, 1599, 1695, 2217
Sau3AI GATC 7 cut(s) 252, 432, 837, 859, 1169, 1374, 1720
Sau96I GGNCC 5 cut(s) 953, 1484, 1529, 1795, 1994
ScaI AGTACT 2 cut(s) 444, 886
SchI GAGTC 5 cut(s) 404, 1157, 1439, 1908, 2242
ScrFI CCNGG 7 cut(s) 167, 746, 878, 1493, 1503, 1533, 1827
SduI GDGCHC 5 cut(s) 571, 1667, 1917, 1934, 2038
SexAI ACCWGGT 1 cut(s) 1531
SfaNI GCATC 3 cut(s) 682, 1315, 1956
SfcI CTRYAG 2 cut(s) 1341, 1770
SgrAI CRCCGGYG 1 cut(s) 1787
SinI GGWCC 3 cut(s) 953, 1529, 1994
SmiMI CAYNNNNRTG 3 cut(s) 825, 1187, 1638
SmlI CTYRAG 2 cut(s) 279, 1658
SmoI CTYRAG 2 cut(s) 279, 1658
SsiI CCGC 7 cut(s) 395, 453, 587, 620, 1274, 1295, 1793
SspI AATATT 1 cut(s) 924
SspMI CTAG 4 cut(s) 446, 698, 797, 2075
SstI GAGCTC 1 cut(s) 1667
StyD4I CCNGG 7 cut(s) 165, 744, 876, 1491, 1501, 1531, 1825
StyI CCWWGG 3 cut(s) 591, 850, 986
TaaI ACNGT 7 cut(s) 250, 573, 884, 1000, 1070, 1435, 2020
TaiI ACGT 1 cut(s) 2226
TaqI TCGA 2 cut(s) 1017, 1394
TaqII GACCGA 2 cut(s) 1835, 2044
TatI WGTACW 2 cut(s) 442, 884
TauI GCSGC 2 cut(s) 590, 1277
TfiI GAWTC 5 cut(s) 10, 991, 1073, 1282, 1308
Tru1I TTAA 6 cut(s) 560, 960, 1175, 1599, 1695, 2217
Tru9I TTAA 6 cut(s) 560, 960, 1175, 1599, 1695, 2217
TscAI CASTG 3 cut(s) 262, 1645, 2151
TseFI GTSAC 6 cut(s) 527, 1351, 1680, 2020, 2086, 2146
TseI GCWGC 9 cut(s) 323, 800, 812, 905, 1343, 1361, 1550, 1649, 1688
Tsp45I GTSAC 6 cut(s) 527, 1351, 1680, 2020, 2086, 2146
TspDTI ATGAA 8 cut(s) 22, 323, 465, 645, 888, 993, 1770, 1965
TspGWI ACGGA 2 cut(s) 651, 1759
TspRI CASTG 3 cut(s) 262, 1645, 2151
VneI GTGCAC 1 cut(s) 1913
VpaK11BI GGWCC 3 cut(s) 953, 1529, 1994
VspI ATTAAT 1 cut(s) 560
XapI RAATTY 5 cut(s) 399, 492, 946, 1390, 1506
XceI RCATGY 1 cut(s) 1352
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XmnI GAANNNNTTC 2 cut(s) 1094, 1761
XspI CTAG 4 cut(s) 446, 698, 797, 2075
ZrmI AGTACT 2 cut(s) 444, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.