FvH4_5g01120

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000302
Physical Location & Seq
Forward (+)
691552 .. 693789
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01120.t1

Sequence Viewer

Length: 2238 bp
ATGGCTGTGATTCCATTTCTTACTCTCATTTTCATGCTTAGCTTCTCTCATGGGATTGCTCAAAAAACAGAGATTTCTCCTTTACAGACATACATTGTCCATGTAATGCAACCAGAGGGTAGAGTTTTTGCCGCAACAGAAGACCTGGAGAGTTGGCATAAATCTTTTCTGCCATCTATGACAGCAAGCTCTGACGATCAAACGCGCCTGCTTTACTCTTACAAAACTGTGATCAGTGGTTTTTCAGCAAGGCTAACTCAGGAGGAAGTGAAAGTGATGGAACTGATGGATGGCTTTGTGGCGGCACATCCTGAACGAGTGTTTCGTCGGAAAACCACACACACTCCCAACTTCTTGGGGCTGAATCGACAAGCGGGAATTTGGAAAGACTCAAACTTTGGGAAGGGAGTCATCATTGGAGTACTGGATGGCGGAGTATTCCCCAGCCACCCTTCATTCAGTGGTGCAGGAATTCCACCTCCACCTGCTAAATGGAAAGGGAGGTGCGACTTCAACGTGTCGGAATGCAATAATAAATTAATTGGAGCACAGTCGTTCAATCTTGCAGCCATGGCATTGAAGGGAGCGAAAGCTGAGCCGCCAATTGATGAAGACGGACATGGAACACACACAGCAAGCACAGCTGGCGGAGCGTTTGTGCAGAATGCTGATGTGCTAGGAAACGCCAAAGGCACAGCAGTTGGAATGGCACCTTACGCTCACCTGGCAATATACAAAGTCTGTTTTGGAGAACCCTGTCCAGAGAGTGATATCCTAGCTGCCCTTGAAGCTGCTGTTCACGATGGTGTTGATGTGATCTCAATCTCCCTTGGAGAAGATTCAGTTCCATTTTTCCAGGACAGTACCGCAATCGGTTCATTTGCAGCAATCCAAAAGGGAATATTTGTTAGTTGTGCAGCTGGAAATTCTGGTCCATTTAACGGCACAATATCTAATGAAGCCCCTTGGATTCTCACAGTTGGAGCAAGCACCCTCGATAGACGCATTGTAGCCTCAGCCGCGCTTGGAAATGGATTAGTATTTGATGGTGAATCTCTCTTTCAGCCAAAAGACTTCCCTTCAACATTATTGCCCCTTGTTTATGCTGGAGTGATTGGCAAAGTAGAGTCTGCATTTTGCGCTGAAGGATCCTTAAAAAACATCAGTGTGAAAGGTAAGGTGGTTGTGTGCGAGCGAGGTGGGGGAATAGGAAGAATTGACAAGGGAGTGGAAGTGAAAAATGCAGGGGGTGCAGCCATGATTCTACTGAATGCGGAAACTGATGGATTCAGTACCTCAGCTGATGCTCATGTTCTTCCTGCAGCACATGTGACCCATGCAGCAGGACTGAACATCAAAGCATACATAAATTCGACAGCAACACCAACAGCGACAATCTTATTCAAAGGAACTGTCATTGGAGACTCAACATCTCCAGCTGTTGCTTCCTTCTCTTCAAGAGGCCCCAACCTGGCAAGTCCAGGAATTCTGAAACCAGACATTATTGGACCAGGAGTAAATGTTCTTGCTGCATGGCCGTTCCCTCTTGACAACAACACAAAAGCAGCATCCACTTTCAATATAATATCCGGAACATCAATGTCATGTCCGCATCTAAGTGGAATTGCAGCTTTGCTTAAAAGCTCTCACCCATACTGGTCACCAGCTGCCATTAAATCTGCTATAATGACTTCTGCGGATCTTATAAATTTGCAAGGCAAACTGATTTTTGATGAAACACTTCAGCCTGCAGATGTCTTAGCCACTGGTGCAGGCCATGTCAACCCATCAAAGGCGACTGATCCAGGACTAGTTTATGATATTCAACCTGATGATTATATTCCATACCTTTGTGGCTTGGGATACAAGGACAGCGAAGTCAGTATCCTGGCGCACAGACCAATAACATGCTCAAAGGTATCAAGCATCCCGGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCAAGCTGGGACCATCTCAGACATTCACAAGAACTGTGACAAATGTTGGTGCCCCATATTCCACTTATTCAGTCAAGGTAAATGCACCACAAGGAGTCTATGTGACTGTCAAACCCAGCACACTTTACTTCACAAAAATGAACCAGAAGATGTCATATTCTGTGACATTCAGTCATGGTTCAGGAGGTAAAGCTGGTTCATTCACTCAGGGATTTATAACATGGGCTTCTGCTAAGCACATTGTCCGGAGTCCAGTTTCTGTCTTACTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

746

Amino Acids

78.28

Weight (kDa)

6.68

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 30 - 108 2.5e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 567 5.7e-44 Subtilase family
PA PF02225 365 - 452 1.7e-12 PA domain
fn3_6 PF17766 647 - 742 1.9e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1706, 2180
AarI CACCTGC 1 cut(s) 493
AasI GACNNNNNNGTC 1 cut(s) 1877
Acc36I ACCTGC 1 cut(s) 493
AccB1I GGYRCC 2 cut(s) 709, 2012
AccII CGCG 2 cut(s) 205, 1022
AccIII TCCGGA 2 cut(s) 1589, 2208
AclWI GGATC 4 cut(s) 1143, 1156, 1707, 1796
AcoI YGGCCR 1 cut(s) 1535
AcsI RAATTY 6 cut(s) 378, 471, 925, 1369, 1485, 1708
AcuI CTGAAG 3 cut(s) 1164, 1727, 2216
AfaI GTAC 3 cut(s) 423, 865, 1294
AfiI CCNNNNNNNGG 3 cut(s) 941, 1471, 1792
AflIII ACRYGT 2 cut(s) 516, 1327
AgsI TTSAA 9 cut(s) 514, 559, 580, 788, 1083, 1405, 1458, 1579, 1826
AhdI GACNNNNNGTC 1 cut(s) 2133
AhlI ACTAGT 1 cut(s) 1810
AjnI CCWGG 8 cut(s) 144, 723, 855, 1470, 1480, 1510, 1804, 1887
AleI CACNNNNGTG 1 cut(s) 804
AloI GAACNNNNNNTCC 2 cut(s) 1506, 1538
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 1 cut(s) 1416
AlwI GGATC 4 cut(s) 1143, 1156, 1707, 1796
AlwNI CAGNNNCTG 1 cut(s) 2222
Aor13HI TCCGGA 2 cut(s) 1589, 2208
AoxI GGCC 3 cut(s) 1462, 1535, 1774
ApoI RAATTY 6 cut(s) 378, 471, 925, 1369, 1485, 1708
ArsI GACNNNNNNTTYG 4 cut(s) 380, 412, 1398, 1430
AseI ATTAAT 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 1740
AspLEI GCGC 4 cut(s) 207, 1024, 1142, 1894
AspS9I GGNCC 4 cut(s) 932, 1463, 1508, 1973
AsuC2I CCSGG 1 cut(s) 1931
AsuHPI GGTGA 4 cut(s) 713, 1061, 1640, 1653
AvaII GGWCC 3 cut(s) 932, 1508, 1973
BaeGI GKGCMC 1 cut(s) 2017
BamHI GGATCC 1 cut(s) 1148
BanI GGYRCC 2 cut(s) 709, 2012
BbsI GAAGAC 2 cut(s) 147, 618
Bbv12I GWGCWC 1 cut(s) 550
BbvCI CCTCAGC 2 cut(s) 1015, 1297
BceAI ACGGC 2 cut(s) 958, 1522
BciT130I CCWGG 8 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889
BciVI GTATCC 2 cut(s) 1856, 1895
BclI TGATCA 1 cut(s) 231
BcnI CCSGG 1 cut(s) 1931
BcoDI GTCTC 1 cut(s) 1416
BcuI ACTAGT 1 cut(s) 1810
BfaI CTAG 3 cut(s) 677, 776, 1811
BfmI CTRYAG 2 cut(s) 1320, 1749
BfuAI ACCTGC 1 cut(s) 493
BfuI GTATCC 2 cut(s) 1856, 1895
BlpI GCTNAGC 3 cut(s) 38, 594, 2196
BmcAI AGTACT 1 cut(s) 423
Bme1390I CCNGG 9 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889, 1931
Bme18I GGWCC 3 cut(s) 932, 1508, 1973
BmeRI GACNNNNNGTC 1 cut(s) 2133
BmgT120I GGNCC 4 cut(s) 932, 1463, 1508, 1973
BmiI GGNNCC 5 cut(s) 711, 1150, 1465, 1974, 2014
BmrFI CCNGG 9 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889, 1931
BmsI GCATC 4 cut(s) 1294, 1577, 1621, 1935
BpiI GAAGAC 2 cut(s) 147, 618
BpmI CTGGAG 3 cut(s) 167, 1128, 1419
Bpu10I CCTNAGC 2 cut(s) 1015, 1297
Bpu1102I GCTNAGC 3 cut(s) 38, 594, 2196
BpuMI CCSGG 1 cut(s) 1931
BsaBI GATNNNNATC 1 cut(s) 821
BsaJI CCNNGG 3 cut(s) 570, 829, 965
BsaWI WCCGGW 2 cut(s) 1589, 2208
Bsc4I CCNNNNNNNGG 3 cut(s) 941, 1471, 1792
Bse1I ACTGG 4 cut(s) 429, 1661, 1771, 2216
Bse8I GATNNNNATC 1 cut(s) 821
BseAI TCCGGA 2 cut(s) 1589, 2208
BseBI CCWGG 8 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889
BseDI CCNNGG 3 cut(s) 570, 829, 965
BseGI GGATG 5 cut(s) 295, 307, 433, 1568, 1926
BseJI GATNNNNATC 1 cut(s) 821
BseLI CCNNNNNNNGG 3 cut(s) 941, 1471, 1792
BseMII CTCAG 6 cut(s) 272, 585, 1029, 1311, 1994, 2183
BseNI ACTGG 4 cut(s) 429, 1661, 1771, 2216
BseSI GKGCMC 1 cut(s) 2017
BseYI CCCAGC 3 cut(s) 443, 1969, 2078
BsgI GTGCAG 5 cut(s) 486, 680, 936, 1272, 1791
Bsh1236I CGCG 2 cut(s) 205, 1022
BshFI GGCC 3 cut(s) 1464, 1537, 1776
BshNI GGYRCC 2 cut(s) 709, 2012
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 3 cut(s) 1590, 1931, 2209
BslFI GGGAC 1 cut(s) 1986
BslI CCNNNNNNNGG 3 cut(s) 941, 1471, 1792
BsmAI GTCTC 1 cut(s) 1416
BsmFI GGGAC 1 cut(s) 1986
BsmI GAATGC 3 cut(s) 530, 670, 1276
BsnI GGCC 3 cut(s) 1464, 1537, 1776
Bsp1286I GDGCHC 2 cut(s) 550, 2017
Bsp13I TCCGGA 2 cut(s) 1589, 2208
Bsp143I GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
Bsp1720I GCTNAGC 3 cut(s) 38, 594, 2196
Bsp19I CCATGG 1 cut(s) 570
BspANI GGCC 3 cut(s) 1464, 1537, 1776
BspCNI CTCAG 6 cut(s) 271, 586, 1028, 1310, 1993, 2182
BspEI TCCGGA 2 cut(s) 1589, 2208
BspFNI CGCG 2 cut(s) 205, 1022
BspLI GGNNCC 5 cut(s) 711, 1150, 1465, 1974, 2014
BspMAI CTGCAG 2 cut(s) 1324, 1753
BspMI ACCTGC 1 cut(s) 493
BspPI GGATC 4 cut(s) 1143, 1156, 1707, 1796
BspT107I GGYRCC 2 cut(s) 709, 2012
BsrI ACTGG 4 cut(s) 429, 1661, 1771, 2216
BssECI CCNNGG 3 cut(s) 570, 829, 965
BssMI GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
BssT1I CCWWGG 3 cut(s) 570, 829, 965
Bst2UI CCWGG 8 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889
Bst4CI ACNGT 7 cut(s) 229, 552, 863, 979, 1414, 1999, 2071
Bst6I CTCTTC 1 cut(s) 1459
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstC8I GCNNGC 8 cut(s) 187, 209, 637, 646, 988, 1193, 1749, 1774
BstDSI CCRYGG 1 cut(s) 570
BstEII GGTNACC 1 cut(s) 1659
BstF5I GGATG 5 cut(s) 295, 307, 433, 1568, 1926
BstFNI CGCG 2 cut(s) 205, 1022
BstHHI GCGC 4 cut(s) 207, 1024, 1142, 1894
BstKTI GATC 6 cut(s) 199, 234, 819, 1151, 1702, 1804
BstMAI GTCTC 1 cut(s) 1416
BstMBI GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
BstNI CCWGG 8 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889
BstNSI RCATGY 2 cut(s) 1331, 1911
BstPI GGTNACC 1 cut(s) 1659
BstSCI CCNGG 9 cut(s) 144, 723, 855, 1470, 1480, 1510, 1804, 1887, 1929
BstSFI CTRYAG 2 cut(s) 1320, 1749
BstSLI GKGCMC 1 cut(s) 2017
BstUI CGCG 2 cut(s) 205, 1022
BstV2I GAAGAC 2 cut(s) 147, 618
BstX2I RGATCY 2 cut(s) 1148, 1699
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 2 cut(s) 1148, 1699
BsuI GTATCC 2 cut(s) 1856, 1895
BsuRI GGCC 3 cut(s) 1464, 1537, 1776
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 5 cut(s) 295, 307, 433, 1568, 1926
BtsIMutI CAGTG 4 cut(s) 241, 466, 1171, 1764
BveI ACCTGC 1 cut(s) 493
Cac8I GCNNGC 8 cut(s) 187, 209, 637, 646, 988, 1193, 1749, 1774
CaiI CAGNNNCTG 1 cut(s) 2222
CfoI GCGC 4 cut(s) 207, 1024, 1142, 1894
Cfr13I GGNCC 4 cut(s) 932, 1463, 1508, 1973
CseI GACGC 1 cut(s) 1011
Csp6I GTAC 3 cut(s) 422, 864, 1293
CviQI GTAC 3 cut(s) 422, 864, 1293
DpnI GATC 6 cut(s) 198, 233, 818, 1150, 1701, 1803
DpnII GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
DrdI GACNNNNNNGTC 1 cut(s) 1877
DriI GACNNNNNGTC 1 cut(s) 2133
DseDI GACNNNNNNGTC 1 cut(s) 1877
EaeI YGGCCR 1 cut(s) 1535
Eam1104I CTCTTC 1 cut(s) 1459
Eam1105I GACNNNNNGTC 1 cut(s) 2133
EarI CTCTTC 1 cut(s) 1459
EciI GGCGGA 2 cut(s) 447, 663
Eco130I CCWWGG 3 cut(s) 570, 829, 965
Eco32I GATATC 1 cut(s) 772
Eco47I GGWCC 3 cut(s) 932, 1508, 1973
Eco57I CTGAAG 3 cut(s) 1164, 1727, 2216
Eco91I GGTNACC 1 cut(s) 1659
EcoO109I RGGNCCY 1 cut(s) 1463
EcoO65I GGTNACC 1 cut(s) 1659
EcoRI GAATTC 2 cut(s) 471, 1485
EcoRII CCWGG 8 cut(s) 144, 723, 855, 1470, 1480, 1510, 1804, 1887
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 3 cut(s) 570, 829, 965
ErhI CCWWGG 3 cut(s) 570, 829, 965
FaqI GGGAC 1 cut(s) 1986
FauI CCCGC 1 cut(s) 367
FbaI TGATCA 1 cut(s) 231
FokI GGATG 5 cut(s) 294, 302, 440, 1555, 1913
FspBI CTAG 3 cut(s) 677, 776, 1811
GlaI GCGC 4 cut(s) 206, 1023, 1141, 1893
GsaI CCCAGC 3 cut(s) 447, 1973, 2082
GsuI CTGGAG 3 cut(s) 167, 1128, 1419
HaeIII GGCC 3 cut(s) 1464, 1537, 1776
HapII CCGG 3 cut(s) 1590, 1931, 2209
HgaI GACGC 1 cut(s) 1011
HhaI GCGC 4 cut(s) 207, 1024, 1142, 1894
Hin6I GCGC 4 cut(s) 205, 1022, 1140, 1892
HinP1I GCGC 4 cut(s) 205, 1022, 1140, 1892
HincII GTYRAC 1 cut(s) 1783
HindII GTYRAC 1 cut(s) 1783
HpaII CCGG 3 cut(s) 1590, 1931, 2209
HphI GGTGA 4 cut(s) 713, 1061, 1640, 1653
Hpy166II GTNNAC 2 cut(s) 799, 1783
Hpy188I TCNGA 5 cut(s) 193, 330, 523, 1491, 1983
Hpy188III TCNNGA 9 cut(s) 260, 311, 761, 800, 1458, 1547, 1590, 2145, 2209
Hpy8I GTNNAC 2 cut(s) 799, 1783
Hpy99I CGWCG 1 cut(s) 330
HpyAV CCTTC 8 cut(s) 397, 462, 574, 1089, 1139, 1459, 1928, 1962
HpyCH4III ACNGT 7 cut(s) 229, 552, 863, 979, 1414, 1999, 2071
HpyCH4IV ACGT 1 cut(s) 516
HpySE526I ACGT 1 cut(s) 516
HspAI GCGC 4 cut(s) 205, 1022, 1140, 1892
Kpn2I TCCGGA 2 cut(s) 1589, 2208
Ksp22I TGATCA 1 cut(s) 231
Kzo9I GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
LmnI GCTCC 4 cut(s) 545, 584, 650, 983
LweI GCATC 4 cut(s) 1294, 1577, 1621, 1935
MaeI CTAG 3 cut(s) 677, 776, 1811
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 5 cut(s) 1330, 1659, 1999, 2065, 2125
MalI GATC 6 cut(s) 198, 233, 818, 1150, 1701, 1803
MboI GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
MboII GAAGA 7 cut(s) 152, 623, 848, 1224, 1307, 1446, 2122
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 2 cut(s) 1148, 1699
MhlI GDGCHC 2 cut(s) 550, 2017
MlyI GAGTC 6 cut(s) 383, 417, 1136, 1418, 2067, 2221
MmeI TCCRAC 4 cut(s) 308, 501, 682, 961
MroI TCCGGA 2 cut(s) 1589, 2208
MroXI GAANNNNTTC 1 cut(s) 1740
MseI TTAA 5 cut(s) 539, 939, 1154, 1638, 1674
MslI CAYNNNNRTG 5 cut(s) 32, 804, 1166, 1617, 2099
MspA1I CMGCKG 5 cut(s) 644, 920, 1301, 1439, 1667
MspI CCGG 3 cut(s) 1590, 1931, 2209
MspR9I CCNGG 9 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889, 1931
MunI CAATTG 1 cut(s) 603
Mva1269I GAATGC 3 cut(s) 530, 670, 1276
MvaI CCWGG 8 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889
MvnI CGCG 2 cut(s) 205, 1022
NciI CCSGG 1 cut(s) 1931
NcoI CCATGG 1 cut(s) 570
NdeII GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
NlaIV GGNNCC 5 cut(s) 711, 1150, 1465, 1974, 2014
NmuCI GTSAC 5 cut(s) 1330, 1659, 1999, 2065, 2125
NspI RCATGY 2 cut(s) 1331, 1911
OliI CACNNNNGTG 1 cut(s) 804
PaqCI CACCTGC 1 cut(s) 493
PciI ACATGT 1 cut(s) 1327
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PctI GAATGC 3 cut(s) 530, 670, 1276
PdmI GAANNNNTTC 1 cut(s) 1740
PfeI GAWTC 7 cut(s) 10, 364, 839, 970, 1052, 1261, 1287
PfoI TCCNGGA 4 cut(s) 855, 1480, 1804, 1929
PleI GAGTC 6 cut(s) 383, 416, 1135, 1418, 2066, 2220
PpsI GAGTC 6 cut(s) 383, 416, 1135, 1418, 2066, 2220
PscI ACATGT 1 cut(s) 1327
PshBI ATTAAT 1 cut(s) 539
PsiI TTATAA 2 cut(s) 1706, 2180
Psp6I CCWGG 8 cut(s) 144, 723, 855, 1470, 1480, 1510, 1804, 1887
PspEI GGTNACC 1 cut(s) 1659
PspFI CCCAGC 3 cut(s) 443, 1969, 2078
PspGI CCWGG 8 cut(s) 144, 723, 855, 1470, 1480, 1510, 1804, 1887
PspN4I GGNNCC 5 cut(s) 711, 1150, 1465, 1974, 2014
PspPI GGNCC 4 cut(s) 932, 1463, 1508, 1973
PsrI GAACNNNNNNTAC 2 cut(s) 2143, 2175
PstI CTGCAG 2 cut(s) 1324, 1753
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 2 cut(s) 1148, 1699
PvuII CAGCTG 5 cut(s) 644, 920, 1301, 1439, 1667
RsaI GTAC 3 cut(s) 423, 865, 1294
RsaNI GTAC 3 cut(s) 422, 864, 1293
RseI CAYNNNNRTG 5 cut(s) 32, 804, 1166, 1617, 2099
SaqAI TTAA 5 cut(s) 539, 939, 1154, 1638, 1674
Sau3AI GATC 6 cut(s) 196, 231, 816, 1148, 1699, 1801
Sau96I GGNCC 4 cut(s) 932, 1463, 1508, 1973
ScaI AGTACT 1 cut(s) 423
SchI GAGTC 6 cut(s) 383, 417, 1136, 1418, 2067, 2221
ScrFI CCNGG 9 cut(s) 146, 725, 857, 1472, 1482, 1512, 1806, 1889, 1931
SduI GDGCHC 2 cut(s) 550, 2017
SfaNI GCATC 4 cut(s) 1294, 1577, 1621, 1935
SfcI CTRYAG 2 cut(s) 1320, 1749
SinI GGWCC 3 cut(s) 932, 1508, 1973
SmiMI CAYNNNNRTG 5 cut(s) 32, 804, 1166, 1617, 2099
SpeI ACTAGT 1 cut(s) 1810
SspI AATATT 1 cut(s) 903
SspMI CTAG 3 cut(s) 677, 776, 1811
StyD4I CCNGG 9 cut(s) 144, 723, 855, 1470, 1480, 1510, 1804, 1887, 1929
StyI CCWWGG 3 cut(s) 570, 829, 965
TaaI ACNGT 7 cut(s) 229, 552, 863, 979, 1414, 1999, 2071
TaiI ACGT 1 cut(s) 519
TaqI TCGA 3 cut(s) 367, 996, 1373
TatI WGTACW 1 cut(s) 421
TauI GCSGC 4 cut(s) 134, 305, 601, 1022
TfiI GAWTC 7 cut(s) 10, 364, 839, 970, 1052, 1261, 1287
Tru1I TTAA 5 cut(s) 539, 939, 1154, 1638, 1674
Tru9I TTAA 5 cut(s) 539, 939, 1154, 1638, 1674
TscAI CASTG 4 cut(s) 241, 466, 1171, 1771
TseFI GTSAC 5 cut(s) 1330, 1659, 1999, 2065, 2125
Tsp45I GTSAC 5 cut(s) 1330, 1659, 1999, 2065, 2125
TspDTI ATGAA 9 cut(s) 22, 444, 624, 867, 972, 1749, 1944, 2117, 2151
TspGWI ACGGA 1 cut(s) 630
TspRI CASTG 4 cut(s) 241, 466, 1171, 1771
VpaK11BI GGWCC 3 cut(s) 932, 1508, 1973
VspI ATTAAT 1 cut(s) 539
XapI RAATTY 6 cut(s) 378, 471, 925, 1369, 1485, 1708
XceI RCATGY 2 cut(s) 1331, 1911
XcmI CCANNNNNNNNNTGG 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 1740
XspI CTAG 3 cut(s) 677, 776, 1811
ZrmI AGTACT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.