Rmu_co8342527.1_g000001

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8342527.1
OG0000302
Physical Location & Seq
Reverse (-)
2 .. 1004
1003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8342527.1_g000001.1.cds

Sequence Viewer

Length: 1003 bp
ctgtgcgatgtaggaacccttaatggagttgaagggaaagtggtgctgtgtgaaagaggtggaggaattggaagaattgccaaaggagaagaagtcaaaagagcaggtggtgctgccatgatcctcatgaacgaagacattgatggctatagcaccttagctgatgctcatgtgcttcctgcaacacatgtgagttatgctgcaggtttgagcatcaaagcctacataaactccacctcaacacctatggccacaatcttgttcaaaggcactgtcatcggtgatcaaaatgctcccgcagtcacttccttttcatcaagaggaccaagcctggcgagcacaggaatcttgaaacctgacatcattggtcccggtgttagcatcttagctgcatggcctgtttcagtggacaatggcacagaatctacggcaacatttaacatggtttcaggtacttcaatgtcatgccctcacctgagtggcattgcagctttgctcaagagctcacaccccgactggtcaccagctgccatcaagtctgcaattgtgacaaccgctgacatagtaaacctcggaggcaaacctattattgatgaaacacttgctccagcagacatctttgccactggtgcaggacatgttaatcctccaaaagcagatgaccctgggctaatctacgacacaaaaccaacggattacattccctacctgtgcggtttgaactacacagacgatcagatacagataatcacccaacaaacagtgaagtgctctgaagtaggagtcatacctgaagcgcagctaaattatccttcattttctattatattccaggacttgtctcagtctcaagattacacaaggacggtgacaaatgttggaccagctaattcaacttacaacttggatcttaagttaccaggtaaaatcgccatgagtgtgagtccttcgcagcttaagtttacagaagttggccagacaattacatacctagcagagttta
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

334

Amino Acids

34.9

Weight (kDa)

4.81

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 2 cut(s) 95, 194
AciI CCGC 3 cut(s) 297, 555, 714
AclWI GGATC 2 cut(s) 115, 915
AcoI YGGCCR 2 cut(s) 249, 973
AcuI CTGAAG 2 cut(s) 795, 813
AfaI GTAC 1 cut(s) 454
AflII CTTAAG 2 cut(s) 911, 956
AflIII ACRYGT 2 cut(s) 187, 637
AgsI TTSAA 6 cut(s) 32, 265, 352, 459, 721, 894
AjnI CCWGG 4 cut(s) 330, 664, 831, 919
AleI CACNNNNGTG 1 cut(s) 477
AluBI AGCT 8 cut(s) 161, 389, 491, 504, 527, 802, 887, 955
AluI AGCT 8 cut(s) 161, 389, 491, 504, 527, 802, 887, 955
Alw21I GWGCWC 3 cut(s) 341, 506, 773
Alw26I GTCTC 2 cut(s) 846, 852
AlwI GGATC 2 cut(s) 115, 915
AoxI GGCC 3 cut(s) 249, 395, 973
ApeKI GCWGC 7 cut(s) 113, 200, 389, 488, 527, 799, 952
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 3 cut(s) 323, 368, 881
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 5 cut(s) 293, 464, 513, 742, 880
AvaII GGWCC 3 cut(s) 323, 368, 881
BalI TGGCCA 2 cut(s) 251, 975
BanII GRGCYC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 3 cut(s) 341, 506, 773
BbvI GCAGC 7 cut(s) 100, 187, 376, 500, 514, 811, 964
BccI CCATC 2 cut(s) 137, 539
BceAI ACGGC 1 cut(s) 444
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 4 cut(s) 332, 666, 833, 921
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 846, 852
BfaI CTAG 1 cut(s) 992
BfmI CTRYAG 2 cut(s) 148, 201
BfrI CTTAAG 2 cut(s) 911, 956
BfuAI ACCTGC 2 cut(s) 95, 194
BisI GCNGC 7 cut(s) 114, 201, 390, 489, 528, 800, 953
BlsI GCNGC 7 cut(s) 115, 202, 391, 490, 529, 801, 954
Bme1390I CCNGG 5 cut(s) 332, 372, 666, 833, 921
Bme18I GGWCC 3 cut(s) 323, 368, 881
BmgT120I GGNCC 3 cut(s) 323, 368, 881
BmiI GGNNCC 2 cut(s) 16, 370
BmrFI CCNGG 5 cut(s) 332, 372, 666, 833, 921
BmsI GCATC 3 cut(s) 154, 222, 390
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 157
BpuEI CTTGAG 2 cut(s) 482, 834
BpuMI CCSGG 1 cut(s) 372
BsaJI CCNNGG 3 cut(s) 571, 664, 665
BsaXI ACNNNNNCTCC 6 cut(s) 78, 108, 215, 245, 589, 619
Bse1I ACTGG 2 cut(s) 521, 631
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 4 cut(s) 332, 666, 833, 921
BseDI CCNNGG 3 cut(s) 571, 664, 665
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 2 cut(s) 467, 857
BseNI ACTGG 2 cut(s) 521, 631
BseXI GCAGC 7 cut(s) 100, 187, 376, 500, 514, 811, 964
BsgI GTGCAG 1 cut(s) 651
BshFI GGCC 3 cut(s) 251, 397, 975
BsiHKAI GWGCWC 3 cut(s) 341, 506, 773
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 354
BsmAI GTCTC 2 cut(s) 846, 852
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 3 cut(s) 251, 397, 975
Bsp1286I GDGCHC 3 cut(s) 341, 506, 773
Bsp143I GATC 4 cut(s) 120, 283, 733, 907
BspACI CCGC 3 cut(s) 297, 555, 714
BspANI GGCC 3 cut(s) 251, 397, 975
BspCNI CTCAG 2 cut(s) 468, 856
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 16, 370
BspMAI CTGCAG 1 cut(s) 205
BspMI ACCTGC 2 cut(s) 95, 194
BspPI GGATC 2 cut(s) 115, 915
BspTI CTTAAG 2 cut(s) 911, 956
BsrDI GCAATG 1 cut(s) 483
BsrI ACTGG 2 cut(s) 521, 631
BssECI CCNNGG 3 cut(s) 571, 664, 665
BssMI GATC 4 cut(s) 120, 283, 733, 907
Bst2UI CCWGG 4 cut(s) 332, 666, 833, 921
Bst4CI ACNGT 3 cut(s) 274, 763, 868
BstAFI CTTAAG 2 cut(s) 911, 956
BstAPI GCANNNNNTGC 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 4 cut(s) 157, 385, 476, 843
BstEII GGTNACC 1 cut(s) 519
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 4 cut(s) 123, 286, 736, 910
BstMAI GTCTC 2 cut(s) 846, 852
BstMBI GATC 4 cut(s) 120, 283, 733, 907
BstMWI GCNNNNNNNGC 3 cut(s) 110, 336, 629
BstNI CCWGG 4 cut(s) 332, 666, 833, 921
BstNSI RCATGY 2 cut(s) 191, 641
BstPI GGTNACC 1 cut(s) 519
BstSCI CCNGG 5 cut(s) 330, 370, 664, 831, 919
BstSFI CTRYAG 2 cut(s) 148, 201
BstV1I GCAGC 7 cut(s) 100, 187, 376, 500, 514, 811, 964
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 907
BstYI RGATCY 1 cut(s) 907
BsuRI GGCC 3 cut(s) 251, 397, 975
BtgZI GCGATG 1 cut(s) 21
BtsIMutI CAGTG 4 cut(s) 270, 411, 624, 768
BveI ACCTGC 2 cut(s) 95, 194
Cac8I GCNNGC 1 cut(s) 337
CciI TCATGA 1 cut(s) 126
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 3 cut(s) 323, 368, 881
CsiI ACCWGGT 1 cut(s) 919
Csp6I GTAC 1 cut(s) 453
CviAII CATG 9 cut(s) 118, 127, 170, 188, 393, 442, 465, 638, 934
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 4 cut(s) 157, 385, 476, 843
DpnI GATC 4 cut(s) 122, 285, 735, 909
DpnII GATC 4 cut(s) 120, 283, 733, 907
EaeI YGGCCR 2 cut(s) 249, 973
Ecl136II GAGCTC 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 506
Eco47I GGWCC 3 cut(s) 323, 368, 881
Eco53kI GAGCTC 1 cut(s) 504
Eco57I CTGAAG 2 cut(s) 795, 813
Eco91I GGTNACC 1 cut(s) 519
EcoICRI GAGCTC 1 cut(s) 504
EcoO65I GGTNACC 1 cut(s) 519
EcoRII CCWGG 4 cut(s) 330, 664, 831, 919
EcoT38I GRGCYC 1 cut(s) 506
FaeI CATG 9 cut(s) 121, 130, 173, 191, 396, 445, 468, 641, 937
FaqI GGGAC 1 cut(s) 354
FatI CATG 9 cut(s) 117, 126, 169, 187, 392, 441, 464, 637, 933
FauI CCCGC 1 cut(s) 304
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 7 cut(s) 114, 201, 390, 489, 528, 800, 953
FriOI GRGCYC 1 cut(s) 506
Fsp4HI GCNGC 7 cut(s) 114, 201, 390, 489, 528, 800, 953
FspBI CTAG 1 cut(s) 992
GlaI GCGC 1 cut(s) 798
GluI GCNGC 7 cut(s) 114, 201, 390, 489, 528, 800, 953
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 3 cut(s) 251, 397, 975
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 799
Hin1II CATG 9 cut(s) 121, 130, 173, 191, 396, 445, 468, 641, 937
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HinfI GANTC 4 cut(s) 345, 422, 783, 943
HpaII CCGG 1 cut(s) 372
HphI GGTGA 5 cut(s) 293, 464, 513, 742, 880
Hpy166II GTNNAC 3 cut(s) 409, 568, 963
Hpy188I TCNGA 3 cut(s) 575, 738, 775
Hpy188III TCNNGA 5 cut(s) 127, 318, 349, 499, 851
Hpy8I GTNNAC 3 cut(s) 409, 568, 963
HpyAV CCTTC 3 cut(s) 26, 822, 957
HpyCH4III ACNGT 3 cut(s) 274, 763, 868
HpyCH4V TGCA 6 cut(s) 182, 203, 392, 488, 542, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 336, 629
HpyF3I CTNAG 4 cut(s) 157, 385, 476, 843
Hsp92II CATG 9 cut(s) 121, 130, 173, 191, 396, 445, 468, 641, 937
HspAI GCGC 1 cut(s) 797
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 4 cut(s) 120, 283, 733, 907
LmnI GCTCC 2 cut(s) 298, 610
Lsp1109I GCAGC 7 cut(s) 100, 187, 376, 500, 514, 811, 964
LweI GCATC 3 cut(s) 154, 222, 390
MabI ACCWGGT 1 cut(s) 919
MaeI CTAG 1 cut(s) 992
MaeIII GTNAC 5 cut(s) 301, 519, 547, 868, 915
MalI GATC 4 cut(s) 122, 285, 735, 909
MboI GATC 4 cut(s) 120, 283, 733, 907
MboII GAAGA 3 cut(s) 84, 101, 146
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 1 cut(s) 907
MhlI GDGCHC 3 cut(s) 341, 506, 773
MlsI TGGCCA 2 cut(s) 251, 975
MluCI AATT 6 cut(s) 66, 75, 543, 805, 889, 981
MluNI TGGCCA 2 cut(s) 251, 975
MlyI GAGTC 2 cut(s) 792, 952
MmeI TCCRAC 1 cut(s) 859
MnlI CCTC 9 cut(s) 50, 56, 134, 247, 314, 480, 569, 581, 657
Mox20I TGGCCA 2 cut(s) 251, 975
MscI TGGCCA 2 cut(s) 251, 975
MseI TTAA 5 cut(s) 21, 438, 642, 912, 957
MslI CAYNNNNRTG 2 cut(s) 477, 938
Msp20I TGGCCA 2 cut(s) 251, 975
MspA1I CMGCKG 2 cut(s) 527, 557
MspCI CTTAAG 2 cut(s) 911, 956
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 5 cut(s) 332, 372, 666, 833, 921
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 4 cut(s) 332, 666, 833, 921
MwoI GCNNNNNNNGC 3 cut(s) 110, 336, 629
NciI CCSGG 1 cut(s) 372
NdeII GATC 4 cut(s) 120, 283, 733, 907
NlaIII CATG 9 cut(s) 121, 130, 173, 191, 396, 445, 468, 641, 937
NlaIV GGNNCC 2 cut(s) 16, 370
NmuCI GTSAC 4 cut(s) 301, 519, 547, 868
NspI RCATGY 2 cut(s) 191, 641
OliI CACNNNNGTG 1 cut(s) 477
PagI TCATGA 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 95
PasI CCCWGGG 1 cut(s) 665
PciI ACATGT 2 cut(s) 187, 637
PfeI GAWTC 2 cut(s) 345, 422
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 7 cut(s) 115, 202, 391, 490, 529, 801, 954
PleI GAGTC 2 cut(s) 791, 951
PpsI GAGTC 2 cut(s) 791, 951
PscI ACATGT 2 cut(s) 187, 637
Psp124BI GAGCTC 1 cut(s) 506
Psp6I CCWGG 4 cut(s) 330, 664, 831, 919
PspEI GGTNACC 1 cut(s) 519
PspGI CCWGG 4 cut(s) 330, 664, 831, 919
PspN4I GGNNCC 2 cut(s) 16, 370
PspPI GGNCC 3 cut(s) 323, 368, 881
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 907
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 2 cut(s) 477, 938
SacI GAGCTC 1 cut(s) 506
SaqAI TTAA 5 cut(s) 21, 438, 642, 912, 957
SatI GCNGC 7 cut(s) 114, 201, 390, 489, 528, 800, 953
Sau3AI GATC 4 cut(s) 120, 283, 733, 907
Sau96I GGNCC 3 cut(s) 323, 368, 881
SchI GAGTC 2 cut(s) 792, 952
ScrFI CCNGG 5 cut(s) 332, 372, 666, 833, 921
SduI GDGCHC 3 cut(s) 341, 506, 773
SexAI ACCWGGT 1 cut(s) 919
SfaNI GCATC 3 cut(s) 154, 222, 390
SfcI CTRYAG 2 cut(s) 148, 201
SinI GGWCC 3 cut(s) 323, 368, 881
SmiMI CAYNNNNRTG 2 cut(s) 477, 938
SmlI CTYRAG 4 cut(s) 497, 849, 911, 956
SmoI CTYRAG 4 cut(s) 497, 849, 911, 956
Sse9I AATT 6 cut(s) 66, 75, 543, 805, 889, 981
SsiI CCGC 3 cut(s) 297, 555, 714
SspMI CTAG 1 cut(s) 992
SstI GAGCTC 1 cut(s) 506
StyD4I CCNGG 5 cut(s) 330, 370, 664, 831, 919
TaaI ACNGT 3 cut(s) 274, 763, 868
TasI AATT 6 cut(s) 66, 75, 543, 805, 889, 981
TfiI GAWTC 2 cut(s) 345, 422
Tru1I TTAA 5 cut(s) 21, 438, 642, 912, 957
Tru9I TTAA 5 cut(s) 21, 438, 642, 912, 957
TscAI CASTG 4 cut(s) 277, 411, 631, 768
TseFI GTSAC 4 cut(s) 301, 519, 547, 868
TseI GCWGC 7 cut(s) 113, 200, 389, 488, 527, 799, 952
Tsp45I GTSAC 4 cut(s) 301, 519, 547, 868
TspDTI ATGAA 4 cut(s) 143, 303, 609, 804
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 4 cut(s) 277, 411, 631, 768
Vha464I CTTAAG 2 cut(s) 911, 956
VpaK11BI GGWCC 3 cut(s) 323, 368, 881
XceI RCATGY 2 cut(s) 191, 641
XspI CTAG 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.