RchiOBHm_Chr7g0200191

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000302
Physical Location & Seq
Reverse (-)
18049527 .. 18050243
717 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17916

Sequence Viewer

Length: 717 bp
ATGATGGAGTGCCACCTAGCTCCCCCGGCGAAATGGAAAGGCAAGTGCAAGTTCAATGGGACAGTGTGTAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGGAAAGAAACAAAAGGAGTCTCTCCATTTGATGAAGAAGGCCATGGCACTCACACCTCCAGCACAGCTGCCAGAAACTTTGTGAAAGGTGCTAGCGTGTTTGGAATGGCAAATGGCACAGCCTCTGGCATTGCACCTTATGCTTACTTAGCCATGTACAAGGTCTGCATCGAGGCAGTTTGTGCCGAAAGTGACATGCTAGCAGCTCTTGACACTGCTGTTGAGGACGGCGTGGATGTGCTCTCACTGTCCATTAGTGATTCCTCAATTCCTTTCCATCAAGATGGGATTGCTATCGGCGCGTTTGGAGCAATTCAAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAACTCTGGCCCTTTCTTTAAGTCCATGTCGAATGAGGCCCCTTGGATTCTCACCGTTAGAGCAAGCACCATTGATAGAAAAATAAGCTCATCAGCACAGCTTGCTATGCTCGGTCCAGGTGACAGAAAAATTCCTGGGCTGGCTATGCTCGATCCTAAAATTTTTAGCACTTCACCATTGCTACCTCTTGTTTATCCAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTCCCTCGGGATCTCTTGTAAATGTTGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

238

Amino Acids

24.87

Weight (kDa)

6.41

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 36 - 174 1.2e-14 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 407
AclWI GGATC 2 cut(s) 599, 700
AcsI RAATTY 4 cut(s) 94, 429, 582, 612
AfaI GTAC 1 cut(s) 263
AgsI TTSAA 3 cut(s) 55, 422, 710
AjnI CCWGG 3 cut(s) 568, 586, 649
AluBI AGCT 8 cut(s) 20, 79, 173, 311, 441, 449, 540, 553
AluI AGCT 8 cut(s) 20, 79, 173, 311, 441, 449, 540, 553
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 2 cut(s) 599, 700
AlwNI CAGNNNCTG 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 688
AoxI GGCC 3 cut(s) 145, 460, 489
ApeKI GCWGC 2 cut(s) 173, 308
ApoI RAATTY 4 cut(s) 94, 429, 582, 612
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 3 cut(s) 461, 490, 566
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 496, 584, 618
AsuNHI GCTAGC 2 cut(s) 197, 304
AvaI CYCGRG 1 cut(s) 688
AvaII GGWCC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 160, 320
BccI CCATC 2 cut(s) 383, 390
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 3 cut(s) 570, 588, 651
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 3 cut(s) 17, 198, 305
BisI GCNGC 2 cut(s) 174, 309
BlsI GCNGC 2 cut(s) 175, 310
Bme1390I CCNGG 4 cut(s) 26, 570, 588, 651
Bme18I GGWCC 1 cut(s) 566
BmeT110I CYCGRG 1 cut(s) 688
BmgT120I GGNCC 3 cut(s) 461, 490, 566
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 4 cut(s) 26, 570, 588, 651
BmsI GCATC 1 cut(s) 282
BmtI GCTAGC 2 cut(s) 201, 308
BpmI CTGGAG 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 398
BsaJI CCNNGG 6 cut(s) 24, 100, 148, 494, 587, 687
Bse3DI GCAATG 2 cut(s) 234, 629
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 3 cut(s) 570, 588, 651
BseDI CCNNGG 6 cut(s) 24, 100, 148, 494, 587, 687
BseGI GGATG 1 cut(s) 346
BseJI GATNNNNATC 1 cut(s) 398
BseMI GCAATG 2 cut(s) 234, 629
BseMII CTCAG 1 cut(s) 666
BseXI GCAGC 2 cut(s) 160, 320
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 3 cut(s) 147, 462, 491
BsiHKAI GWGCWC 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 688
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 2 cut(s) 73, 669
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 2 cut(s) 73, 669
BsnI GGCC 3 cut(s) 147, 462, 491
BsoBI CYCGRG 1 cut(s) 688
Bsp1286I GDGCHC 1 cut(s) 348
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 2 cut(s) 604, 692
Bsp19I CCATGG 1 cut(s) 148
BspANI GGCC 3 cut(s) 147, 462, 491
BspCNI CTCAG 1 cut(s) 667
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 492
BspOI GCTAGC 2 cut(s) 201, 308
BspPI GGATC 2 cut(s) 599, 700
BsrDI GCAATG 2 cut(s) 234, 629
BsrGI TGTACA 1 cut(s) 261
BssECI CCNNGG 6 cut(s) 24, 100, 148, 494, 587, 687
BssMI GATC 2 cut(s) 604, 692
BssT1I CCWWGG 3 cut(s) 100, 148, 494
Bst2UI CCWGG 3 cut(s) 570, 588, 651
Bst4CI ACNGT 3 cut(s) 64, 354, 508
BstAPI GCANNNNNTGC 3 cut(s) 245, 287, 554
BstAUI TGTACA 1 cut(s) 261
BstC8I GCNNGC 5 cut(s) 199, 306, 517, 555, 594
BstDEI CTNAG 2 cut(s) 253, 675
BstDSI CCRYGG 1 cut(s) 148
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 1 cut(s) 407
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 2 cut(s) 607, 695
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 604, 692
BstMWI GCNNNNNNNGC 9 cut(s) 26, 245, 254, 287, 404, 413, 554, 559, 598
BstNI CCWGG 3 cut(s) 570, 588, 651
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 4 cut(s) 24, 568, 586, 649
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 2 cut(s) 160, 320
BstX2I RGATCY 1 cut(s) 692
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 1 cut(s) 692
BsuRI GGCC 3 cut(s) 147, 462, 491
BtgI CCRYGG 1 cut(s) 148
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 3 cut(s) 69, 318, 350
Cac8I GCNNGC 5 cut(s) 199, 306, 517, 555, 594
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 461, 490, 566
Csp6I GTAC 1 cut(s) 262
CviAII CATG 4 cut(s) 149, 259, 301, 478
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 2 cut(s) 253, 675
DpnI GATC 2 cut(s) 606, 694
DpnII GATC 2 cut(s) 604, 692
Eco130I CCWWGG 3 cut(s) 100, 148, 494
Eco47I GGWCC 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 490
EcoRII CCWGG 3 cut(s) 568, 586, 649
EcoT14I CCWWGG 3 cut(s) 100, 148, 494
ErhI CCWWGG 3 cut(s) 100, 148, 494
FaeI CATG 4 cut(s) 152, 262, 304, 481
FaiI YATR 7 cut(s) 150, 246, 260, 302, 479, 560, 599
FaqI GGGAC 2 cut(s) 73, 669
FatI CATG 4 cut(s) 148, 258, 300, 477
Fnu4HI GCNGC 2 cut(s) 174, 309
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 174, 309
FspBI CTAG 3 cut(s) 17, 198, 305
GlaI GCGC 1 cut(s) 406
GluI GCNGC 2 cut(s) 174, 309
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 3 cut(s) 147, 462, 491
HapII CCGG 1 cut(s) 26
HhaI GCGC 1 cut(s) 407
Hin1II CATG 4 cut(s) 152, 262, 304, 481
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 3 cut(s) 123, 365, 499
HpaII CCGG 1 cut(s) 26
HphI GGTGA 3 cut(s) 496, 584, 618
Hpy188III TCNNGA 3 cut(s) 314, 386, 690
HpyAV CCTTC 2 cut(s) 137, 704
HpyCH4III ACNGT 3 cut(s) 64, 354, 508
HpyCH4V TGCA 5 cut(s) 48, 89, 239, 273, 656
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 245, 254, 287, 404, 413, 554, 559, 598
HpyF3I CTNAG 2 cut(s) 253, 675
Hsp92II CATG 4 cut(s) 152, 262, 304, 481
HspAI GCGC 1 cut(s) 405
Kzo9I GATC 2 cut(s) 604, 692
LmnI GCTCC 2 cut(s) 25, 413
Lsp1109I GCAGC 2 cut(s) 160, 320
LweI GCATC 1 cut(s) 282
MaeI CTAG 3 cut(s) 17, 198, 305
MaeIII GTNAC 3 cut(s) 68, 296, 572
MalI GATC 2 cut(s) 606, 694
MboI GATC 2 cut(s) 604, 692
MboII GAAGA 1 cut(s) 152
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 6 cut(s) 94, 372, 417, 429, 582, 612
MlyI GAGTC 1 cut(s) 132
MnlI CCTC 8 cut(s) 172, 238, 271, 322, 379, 481, 648, 697
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 3 cut(s) 173, 441, 449
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 4 cut(s) 26, 570, 588, 651
MvaI CCWGG 3 cut(s) 570, 588, 651
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 9 cut(s) 26, 245, 254, 287, 404, 413, 554, 559, 598
NciI CCSGG 1 cut(s) 26
NcoI CCATGG 1 cut(s) 148
NdeII GATC 2 cut(s) 604, 692
NheI GCTAGC 2 cut(s) 197, 304
NlaIII CATG 4 cut(s) 152, 262, 304, 481
NlaIV GGNNCC 1 cut(s) 492
NmuCI GTSAC 2 cut(s) 296, 572
NspI RCATGY 1 cut(s) 304
PfeI GAWTC 2 cut(s) 365, 499
PkrI GCNGC 2 cut(s) 175, 310
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp6I CCWGG 3 cut(s) 568, 586, 649
PspGI CCWGG 3 cut(s) 568, 586, 649
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 3 cut(s) 461, 490, 566
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 1 cut(s) 692
PvuII CAGCTG 3 cut(s) 173, 441, 449
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 2 cut(s) 174, 309
Sau3AI GATC 2 cut(s) 604, 692
Sau96I GGNCC 3 cut(s) 461, 490, 566
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 4 cut(s) 26, 570, 588, 651
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 282
SinI GGWCC 1 cut(s) 566
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 6 cut(s) 94, 372, 417, 429, 582, 612
SspMI CTAG 3 cut(s) 17, 198, 305
StyD4I CCNGG 4 cut(s) 24, 568, 586, 649
StyI CCWWGG 3 cut(s) 100, 148, 494
TaaI ACNGT 3 cut(s) 64, 354, 508
TaqI TCGA 3 cut(s) 276, 482, 603
TaqII GACCGA 1 cut(s) 554
TasI AATT 6 cut(s) 94, 372, 417, 429, 582, 612
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 2 cut(s) 365, 499
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 3 cut(s) 69, 325, 357
TseFI GTSAC 2 cut(s) 296, 572
TseI GCWGC 2 cut(s) 173, 308
Tsp45I GTSAC 2 cut(s) 296, 572
TspDTI ATGAA 1 cut(s) 153
TspRI CASTG 3 cut(s) 69, 325, 357
VpaK11BI GGWCC 1 cut(s) 566
XapI RAATTY 4 cut(s) 94, 429, 582, 612
XceI RCATGY 1 cut(s) 304
XspI CTAG 3 cut(s) 17, 198, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.