Rw6G012950

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000302
Physical Location & Seq
Forward (+)
23910017 .. 23912286
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G012950.1

Sequence Viewer

Length: 2163 bp
ATGCAGATCATACACTTTGGTTTTAGTTTCATGTTTTACATGTCACTAGTAGTTGCTGATGAAAAAGCGGCAGCATTGGAGTCACTAGCCAAGACAAAGAGCATGCAAACTTATATCGTTTGGGTGGAAAAGCCAATCAGCGATTTGATTGCACAATCTGAACATGAAGATTTAGGGAGTTGGTACCAGACATTTTTGCCCCAAACTATTGCAAACTCAAACCAGCTTGAGAAGCCACGAATGGTTTATGCATACCACAATGTGGCAACTGGCTTCGCAGCAAAGCTGACAGCAAAGGAAGTTGAAGAGATGGAAAAGAAAGATGGGTTTATCTCCGCTCATCCAGAACGAAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAGCAAGGAATGGGACTAGGTAAAGGGCCTAACTATGGTGAAGGTGTGATTATTGGAATTTTGGATAGTGGAATATTCCCAGACCATCCTTCATTTAGTGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAGTGTGATTTCAGCGGGACAGTCTGCAATAACAAGCTTATAGGTGCCAGAAGTTTTAATGGTGGAAAAACTACTGGAAGCCCTCCAGTTGACGATGAAGGCCATGGAACTCACACGTCAAGCACAGCTGGAGGAAATTTTGTGAAAGGTGCCAGCGTGTTTGGAATGGCCAACGGCACAGCAACTGGCATGGCACCTCATGCTCACTTGGCAATGTACAAAGTATGCTCAGACGAGGGTTGTTCTGAATCAGACATTATAGCTGCTATGGACACTGCTGTTGATGATGGCGTGGATGTGTTATCCCTTTCAATTGGTGGTGGCTCAGCTTATTTTTATGCTGATGGAATTGCAGTCGGCGCATTTGGAGCAATTCAGAAGGGAATCTTTGTCAGCTGTTCAGCTGGAAATGCTGGCCCTGACTATATGACTTTATCTAATGAAGCTCCATGGATTCTCACGGTTGGAGCAAGCACGATTGACAGAAACATAAGAGCAACAGCAAAGCTTGGAAATGGGCAAGAATATGACGGGGAGTCAGTATTCCAGCCTAAAGATTTTAGTTCAAAACAGTTAGCTCTTGTTTATGCAGGTGCATATAGCAATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTAAATATGTTGAAGGGAAAGTAGTGGTGTGTGAGGTAGGTGGAGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTCGCTGGCTATACCACCATAGCAGACATTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCAGGGGTGAGCATCAAAACCTATATAAATTCAACCTCAACTCCTACAGCGACAATCTTATTCAAAGGCACTATCATCGCTGCGTGGCCTTTTTCGGTGGATAATGCTTTAGAATCTAAGGCCACATTTGACATTATTTCAGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCTGCTGCCATTAAGTCTGCAATGATGACAACTGCTGAAGTAAACAACCTCGCTGGCTCGGCCATTCTTGATCAAACACTTCTTGCAGCAGACCTCTTTGCCATTGGCGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACATACAACCAGAGGACTACATTCCTTACTTGTGTGGTTTGAATTACACAAGCAAACAGATATCGGCAATCACCCAACAAAAAGTGTCGTGCTCTAAAGTAGGAGTCATACCAGAAGCACAACTAAACTATCCCACATTTTGTATCTATATAGAGTCTGGTGGTGAGCCTCAGAATTACACAAGAACCTTGACCAATGTTGGCCCAGCTAATTCAACTTACAAATTGGCTCCCCTAAGACTACATAAAATGGACATCACTGTGCTACCTGAGGTGCTTACATTTACAGAGGTTAACCAAAAATTGACATACCGTGTGGTGTTTGCTGCACAAGATGGAGCTGGGAAAGATGGTATACCATTTTCTCAGGGATATTTGAGTTGGGTGTCTCATCAGCATTCTGTTAACAGCCAAATATCTGTGGTCTTTGACTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

720

Amino Acids

76.46

Weight (kDa)

5.39

Isoelectric Point (pI)

30.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 122 8e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 145 - 537 5.1e-42 Subtilase family
PA PF02225 372 - 455 1.1e-09 PA domain
fn3_6 PF17766 615 - 715 3.5e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1115, 1576
Acc36I ACCTGC 2 cut(s) 1115, 1576
Acc65I GGTACC 1 cut(s) 183
AccB1I GGYRCC 4 cut(s) 183, 578, 683, 727
AccB7I CCANNNNNTGG 1 cut(s) 262
AccBSI CCGCTC 1 cut(s) 338
AccI GTMKAC 1 cut(s) 2077
AciI CCGC 3 cut(s) 68, 336, 549
AcoI YGGCCR 2 cut(s) 702, 1626
AcsI RAATTY 3 cut(s) 453, 670, 1381
AcuI CTGAAG 1 cut(s) 1623
AcvI CACGTG 1 cut(s) 1324
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 4 cut(s) 185, 507, 752, 1498
AfiI CCNNNNNNNGG 2 cut(s) 262, 431
AflIII ACRYGT 3 cut(s) 39, 648, 1339
AhdI GACNNNNNGTC 1 cut(s) 1069
AhlI ACTAGT 1 cut(s) 46
AjiI CACGTC 1 cut(s) 651
AjnI CCWGG 1 cut(s) 1708
AleI CACNNNNGTG 1 cut(s) 1521
Alw21I GWGCWC 2 cut(s) 1550, 1817
Alw26I GTCTC 1 cut(s) 2115
AlwNI CAGNNNCTG 1 cut(s) 719
AoxI GGCC 9 cut(s) 422, 634, 702, 949, 1439, 1473, 1626, 1678, 1924
ApoI RAATTY 3 cut(s) 453, 670, 1381
Asp718I GGTACC 1 cut(s) 183
AspLEI GCGC 2 cut(s) 896, 1676
AspS9I GGNCC 4 cut(s) 422, 950, 1167, 1925
AsuHPI GGTGA 6 cut(s) 446, 1372, 1508, 1557, 1786, 1898
AvaII GGWCC 1 cut(s) 1167
AxyI CCTNAGG 1 cut(s) 1992
BalI TGGCCA 1 cut(s) 704
BanI GGYRCC 4 cut(s) 183, 578, 683, 727
BanII GRGCYC 2 cut(s) 1550, 1716
BbrPI CACGTG 1 cut(s) 1324
Bbv12I GWGCWC 2 cut(s) 1550, 1817
BccI CCATC 7 cut(s) 304, 317, 489, 815, 872, 2051, 2066
BceAI ACGGC 1 cut(s) 724
BcgI CGANNNNNNTGC 4 cut(s) 131, 165, 1411, 1445
BciT130I CCWGG 1 cut(s) 1710
BclI TGATCA 1 cut(s) 1636
BcoDI GTCTC 1 cut(s) 2115
BcuI ACTAGT 1 cut(s) 46
BfaI CTAG 3 cut(s) 47, 86, 413
BfmI CTRYAG 3 cut(s) 395, 1353, 1398
BfuAI ACCTGC 2 cut(s) 1115, 1576
BlpI GCTNAGC 1 cut(s) 859
Bme1390I CCNGG 1 cut(s) 1710
Bme18I GGWCC 1 cut(s) 1167
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmgBI CACGTC 1 cut(s) 651
BmgT120I GGNCC 4 cut(s) 422, 950, 1167, 1925
BmiI GGNNCC 7 cut(s) 185, 580, 685, 729, 1168, 1169, 1953
BmrFI CCNGG 1 cut(s) 1710
BmsI GCATC 1 cut(s) 1374
BpmI CTGGAG 3 cut(s) 501, 603, 684
Bpu1102I GCTNAGC 1 cut(s) 859
BpuEI CTTGAG 2 cut(s) 248, 1526
BsaAI YACGTR 1 cut(s) 1324
BsaJI CCNNGG 4 cut(s) 637, 983, 1708, 1709
BsaXI ACNNNNNCTCC 2 cut(s) 1378, 1408
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 431
Bse1I ACTGG 5 cut(s) 274, 613, 620, 724, 1565
Bse21I CCTNAGG 1 cut(s) 1992
Bse3DI GCAATG 4 cut(s) 753, 1144, 1527, 1593
BseBI CCWGG 1 cut(s) 1710
BseDI CCNNGG 4 cut(s) 637, 983, 1708, 1709
BseGI GGATG 3 cut(s) 340, 481, 835
BseLI CCNNNNNNNGG 2 cut(s) 262, 431
BseMI GCAATG 4 cut(s) 753, 1144, 1527, 1593
BseMII CTCAG 5 cut(s) 777, 873, 1907, 1983, 2102
BseNI ACTGG 5 cut(s) 274, 613, 620, 724, 1565
BseRI GAGGAG 1 cut(s) 504
BseYI CCCAGC 2 cut(s) 1927, 2063
BsgI GTGCAG 1 cut(s) 2034
BshFI GGCC 9 cut(s) 424, 636, 704, 951, 1441, 1475, 1628, 1680, 1926
BshNI GGYRCC 4 cut(s) 183, 578, 683, 727
BsiHKAI GWGCWC 2 cut(s) 1550, 1817
BsiSI CCGG 1 cut(s) 1331
BslFI GGGAC 3 cut(s) 423, 565, 1153
BslI CCNNNNNNNGG 2 cut(s) 262, 431
BsmAI GTCTC 1 cut(s) 2115
BsmFI GGGAC 3 cut(s) 423, 565, 1153
BsmI GAATGC 1 cut(s) 2119
BsnI GGCC 9 cut(s) 424, 636, 704, 951, 1441, 1475, 1628, 1680, 1926
Bsp1286I GDGCHC 3 cut(s) 1550, 1716, 1817
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 2 cut(s) 6, 1636
Bsp1720I GCTNAGC 1 cut(s) 859
Bsp19I CCATGG 2 cut(s) 637, 983
BspACI CCGC 3 cut(s) 68, 336, 549
BspANI GGCC 9 cut(s) 424, 636, 704, 951, 1441, 1475, 1628, 1680, 1926
BspCNI CTCAG 5 cut(s) 776, 872, 1906, 1984, 2101
BspLI GGNNCC 7 cut(s) 185, 580, 685, 729, 1168, 1169, 1953
BspMAI CTGCAG 2 cut(s) 399, 1357
BspMI ACCTGC 2 cut(s) 1115, 1576
BspT107I GGYRCC 4 cut(s) 183, 578, 683, 727
BsrBI CCGCTC 1 cut(s) 338
BsrDI GCAATG 4 cut(s) 753, 1144, 1527, 1593
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 5 cut(s) 274, 613, 620, 724, 1565
BssECI CCNNGG 4 cut(s) 637, 983, 1708, 1709
BssMI GATC 2 cut(s) 6, 1636
BssNAI GTATAC 1 cut(s) 2078
BssT1I CCWWGG 2 cut(s) 637, 983
Bst1107I GTATAC 1 cut(s) 2078
Bst2UI CCWGG 1 cut(s) 1710
Bst4CI ACNGT 5 cut(s) 556, 997, 1107, 1984, 2036
Bst6I CTCTTC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 750
BstBAI YACGTR 1 cut(s) 1324
BstC8I GCNNGC 7 cut(s) 104, 688, 949, 1006, 1297, 1621, 1678
BstDEI CTNAG 8 cut(s) 763, 859, 1470, 1520, 1893, 1958, 1992, 2088
BstDSI CCRYGG 2 cut(s) 637, 983
BstEII GGTNACC 1 cut(s) 1563
BstF5I GGATG 3 cut(s) 340, 481, 835
BstHHI GCGC 2 cut(s) 896, 1676
BstKTI GATC 2 cut(s) 9, 1639
BstMAI GTCTC 1 cut(s) 2115
BstMBI GATC 2 cut(s) 6, 1636
BstMWI GCNNNNNNNGC 8 cut(s) 232, 734, 743, 893, 902, 944, 1625, 1673
BstNI CCWGG 1 cut(s) 1710
BstNSI RCATGY 3 cut(s) 43, 106, 1343
BstPI GGTNACC 1 cut(s) 1563
BstSCI CCNGG 1 cut(s) 1708
BstSFI CTRYAG 3 cut(s) 395, 1353, 1398
BstXI CCANNNNNNTGG 1 cut(s) 525
BstZ17I GTATAC 1 cut(s) 2078
Bsu36I CCTNAGG 1 cut(s) 1992
BsuRI GGCC 9 cut(s) 424, 636, 704, 951, 1441, 1475, 1628, 1680, 1926
BtgI CCRYGG 2 cut(s) 637, 983
BtgZI GCGATG 2 cut(s) 1173, 1414
BtrI CACGTC 1 cut(s) 651
BtsCI GGATG 3 cut(s) 340, 481, 835
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 2 cut(s) 807, 1980
BveI ACCTGC 2 cut(s) 1115, 1576
Cac8I GCNNGC 7 cut(s) 104, 688, 949, 1006, 1297, 1621, 1678
CaiI CAGNNNCTG 1 cut(s) 719
CfoI GCGC 2 cut(s) 896, 1676
Cfr13I GGNCC 4 cut(s) 422, 950, 1167, 1925
Csp6I GTAC 4 cut(s) 184, 506, 751, 1497
CviQI GTAC 4 cut(s) 184, 506, 751, 1497
DdeI CTNAG 8 cut(s) 763, 859, 1470, 1520, 1893, 1958, 1992, 2088
DpnI GATC 2 cut(s) 8, 1638
DpnII GATC 2 cut(s) 6, 1636
DraIII CACNNNGTG 1 cut(s) 262
DriI GACNNNNNGTC 1 cut(s) 1069
EaeI YGGCCR 2 cut(s) 702, 1626
Eam1104I CTCTTC 1 cut(s) 300
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 1548
Eco130I CCWWGG 2 cut(s) 637, 983
Eco24I GRGCYC 2 cut(s) 1550, 1716
Eco32I GATATC 1 cut(s) 1785
Eco47I GGWCC 1 cut(s) 1167
Eco53kI GAGCTC 1 cut(s) 1548
Eco57I CTGAAG 1 cut(s) 1623
Eco72I CACGTG 1 cut(s) 1324
Eco81I CCTNAGG 1 cut(s) 1992
Eco91I GGTNACC 1 cut(s) 1563
EcoICRI GAGCTC 1 cut(s) 1548
EcoO109I RGGNCCY 2 cut(s) 422, 1167
EcoO65I GGTNACC 1 cut(s) 1563
EcoRII CCWGG 1 cut(s) 1708
EcoRV GATATC 1 cut(s) 1785
EcoT14I CCWWGG 2 cut(s) 637, 983
EcoT22I ATGCAT 1 cut(s) 253
EcoT38I GRGCYC 2 cut(s) 1550, 1716
ErhI CCWWGG 2 cut(s) 637, 983
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FaqI GGGAC 3 cut(s) 423, 565, 1153
FauI CCCGC 1 cut(s) 542
FbaI TGATCA 1 cut(s) 1636
FblI GTMKAC 1 cut(s) 2077
FokI GGATG 3 cut(s) 327, 468, 842
FriOI GRGCYC 2 cut(s) 1550, 1716
FspBI CTAG 3 cut(s) 47, 86, 413
GlaI GCGC 2 cut(s) 895, 1675
GsaI CCCAGC 2 cut(s) 1931, 2067
GsuI CTGGAG 3 cut(s) 501, 603, 684
HaeIII GGCC 9 cut(s) 424, 636, 704, 951, 1441, 1475, 1628, 1680, 1926
HapII CCGG 1 cut(s) 1331
HhaI GCGC 2 cut(s) 896, 1676
Hin6I GCGC 2 cut(s) 894, 1674
HinP1I GCGC 2 cut(s) 894, 1674
HincII GTYRAC 5 cut(s) 625, 1282, 1687, 2017, 2128
HindII GTYRAC 5 cut(s) 625, 1282, 1687, 2017, 2128
HindIII AAGCTT 2 cut(s) 569, 1040
HpaI GTTAAC 3 cut(s) 1687, 2017, 2128
HpaII CCGG 1 cut(s) 1331
HphI GGTGA 6 cut(s) 446, 1372, 1508, 1557, 1786, 1898
Hpy166II GTNNAC 7 cut(s) 625, 1282, 1609, 1687, 2017, 2078, 2128
Hpy188I TCNGA 6 cut(s) 160, 766, 781, 787, 912, 1896
Hpy188III TCNNGA 3 cut(s) 344, 1543, 1634
Hpy8I GTNNAC 7 cut(s) 625, 1282, 1609, 1687, 2017, 2078, 2128
HpyAV CCTTC 7 cut(s) 431, 494, 495, 626, 907, 1178, 1701
HpyCH4III ACNGT 5 cut(s) 556, 997, 1107, 1984, 2036
HpyCH4IV ACGT 2 cut(s) 650, 1323
HpyF10VI GCNNNNNNNGC 8 cut(s) 232, 734, 743, 893, 902, 944, 1625, 1673
HpyF3I CTNAG 8 cut(s) 763, 859, 1470, 1520, 1893, 1958, 1992, 2088
HpySE526I ACGT 2 cut(s) 650, 1323
HspAI GCGC 2 cut(s) 894, 1674
KflI GGGWCCC 1 cut(s) 1167
KpnI GGTACC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 1636
KspAI GTTAAC 3 cut(s) 1687, 2017, 2128
Kzo9I GATC 2 cut(s) 6, 1636
LmnI GCTCC 5 cut(s) 902, 985, 1001, 1957, 2060
LweI GCATC 1 cut(s) 1374
MaeI CTAG 3 cut(s) 47, 86, 413
MaeII ACGT 2 cut(s) 650, 1323
MaeIII GTNAC 4 cut(s) 42, 81, 1346, 1563
MalI GATC 2 cut(s) 8, 1638
MbiI CCGCTC 1 cut(s) 338
MboI GATC 2 cut(s) 6, 1636
MboII GAAGA 3 cut(s) 179, 317, 1236
MfeI CAATTG 2 cut(s) 359, 846
MhlI GDGCHC 3 cut(s) 1550, 1716, 1817
MlsI TGGCCA 1 cut(s) 704
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 4 cut(s) 89, 1079, 1836, 1886
MmeI TCCRAC 1 cut(s) 979
Mox20I TGGCCA 1 cut(s) 704
Mph1103I ATGCAT 1 cut(s) 253
MscI TGGCCA 1 cut(s) 704
MseI TTAA 6 cut(s) 591, 1173, 1578, 1686, 2016, 2127
MslI CAYNNNNRTG 3 cut(s) 1322, 1521, 1982
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 4 cut(s) 549, 662, 930, 938
MspI CCGG 1 cut(s) 1331
MspR9I CCNGG 1 cut(s) 1710
MunI CAATTG 2 cut(s) 359, 846
Mva1269I GAATGC 1 cut(s) 2119
MvaI CCWGG 1 cut(s) 1710
MwoI GCNNNNNNNGC 8 cut(s) 232, 734, 743, 893, 902, 944, 1625, 1673
NcoI CCATGG 2 cut(s) 637, 983
NdeII GATC 2 cut(s) 6, 1636
NlaIV GGNNCC 7 cut(s) 185, 580, 685, 729, 1168, 1169, 1953
NmeAIII GCCGAG 1 cut(s) 1604
NmuCI GTSAC 3 cut(s) 42, 81, 1563
NsiI ATGCAT 1 cut(s) 253
NspI RCATGY 3 cut(s) 43, 106, 1343
OliI CACNNNNGTG 1 cut(s) 1521
PaeI GCATGC 1 cut(s) 106
PaqCI CACCTGC 2 cut(s) 1115, 1576
PasI CCCWGGG 1 cut(s) 1709
PciI ACATGT 2 cut(s) 39, 1339
PctI GAATGC 1 cut(s) 2119
PfeI GAWTC 6 cut(s) 782, 918, 988, 1142, 1273, 1466
PflMI CCANNNNNTGG 1 cut(s) 262
PleI GAGTC 4 cut(s) 88, 1078, 1835, 1885
PmaCI CACGTG 1 cut(s) 1324
PmlI CACGTG 1 cut(s) 1324
PpsI GAGTC 4 cut(s) 88, 1078, 1835, 1885
Ppu21I YACGTR 1 cut(s) 1324
PpuMI RGGWCCY 1 cut(s) 1167
PscI ACATGT 2 cut(s) 39, 1339
Psp124BI GAGCTC 1 cut(s) 1550
Psp5II RGGWCCY 1 cut(s) 1167
Psp6I CCWGG 1 cut(s) 1708
PspCI CACGTG 1 cut(s) 1324
PspEI GGTNACC 1 cut(s) 1563
PspFI CCCAGC 2 cut(s) 1927, 2063
PspGI CCWGG 1 cut(s) 1708
PspN4I GGNNCC 7 cut(s) 185, 580, 685, 729, 1168, 1169, 1953
PspPI GGNCC 4 cut(s) 422, 950, 1167, 1925
PspPPI RGGWCCY 1 cut(s) 1167
PstI CTGCAG 2 cut(s) 399, 1357
PstNI CAGNNNCTG 1 cut(s) 719
PvuII CAGCTG 3 cut(s) 662, 930, 938
RsaI GTAC 4 cut(s) 185, 507, 752, 1498
RsaNI GTAC 4 cut(s) 184, 506, 751, 1497
RseI CAYNNNNRTG 3 cut(s) 1322, 1521, 1982
SacI GAGCTC 1 cut(s) 1550
SaqAI TTAA 6 cut(s) 591, 1173, 1578, 1686, 2016, 2127
Sau3AI GATC 2 cut(s) 6, 1636
Sau96I GGNCC 4 cut(s) 422, 950, 1167, 1925
SchI GAGTC 4 cut(s) 89, 1079, 1836, 1886
ScrFI CCNGG 1 cut(s) 1710
SduI GDGCHC 3 cut(s) 1550, 1716, 1817
SfaNI GCATC 1 cut(s) 1374
SfcI CTRYAG 3 cut(s) 395, 1353, 1398
SinI GGWCC 1 cut(s) 1167
SmiMI CAYNNNNRTG 3 cut(s) 1322, 1521, 1982
SmlI CTYRAG 2 cut(s) 227, 1541
SmoI CTYRAG 2 cut(s) 227, 1541
SpeI ACTAGT 1 cut(s) 46
SphI GCATGC 1 cut(s) 106
SsiI CCGC 3 cut(s) 68, 336, 549
SspI AATATT 1 cut(s) 471
SspMI CTAG 3 cut(s) 47, 86, 413
SstI GAGCTC 1 cut(s) 1550
StyD4I CCNGG 1 cut(s) 1708
StyI CCWWGG 2 cut(s) 637, 983
TaaI ACNGT 5 cut(s) 556, 997, 1107, 1984, 2036
TaiI ACGT 2 cut(s) 653, 1326
TatI WGTACW 1 cut(s) 750
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 6 cut(s) 782, 918, 988, 1142, 1273, 1466
Tru1I TTAA 6 cut(s) 591, 1173, 1578, 1686, 2016, 2127
Tru9I TTAA 6 cut(s) 591, 1173, 1578, 1686, 2016, 2127
TscAI CASTG 2 cut(s) 814, 1987
TseFI GTSAC 3 cut(s) 42, 81, 1563
Tsp45I GTSAC 3 cut(s) 42, 81, 1563
TspDTI ATGAA 8 cut(s) 19, 75, 180, 477, 513, 645, 990, 1134
TspRI CASTG 2 cut(s) 814, 1987
Van91I CCANNNNNTGG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 1167
XapI RAATTY 3 cut(s) 453, 670, 1381
XceI RCATGY 3 cut(s) 43, 106, 1343
XmiI GTMKAC 1 cut(s) 2077
XspI CTAG 3 cut(s) 47, 86, 413
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.