FvH4_5g01093

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000302
Physical Location & Seq
Forward (+)
687597 .. 690260
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01093.t1

Sequence Viewer

Length: 2313 bp
ATGGAGAACAAAAAGTTTGTAGCCCTGCAGATCATATATCTTCTTGGCTTGAGTTTCGCCTTCTGCCTATCTCTAGCAATTACTGATGGAGGCATGGGATTACTCCACGCTAAAGCTGAATCCAATGTAGAAGAGGAAAAGAGTGGCATGCAAACATACATTGTTTGGGTGAAAAAGCCTGTAAGAAAGCTATTTTCAGAGTCCCATCAAGACTTAGAGGGTTGGTACAAGTCATTTCTGCCGAAAACTTTTACAAACTCAATGGAGGTGATGGAACAACGATTGGTTTACGCATATCACCATGTGGCTACTGGCTTTGCAGCAAAGCTGACAGCAGAGGAAGTGAAAGAAATGGCGGTGAAAGATGGGTTTATATCAGCTCATCAAGAAAGGATTCTCCCATTGCACACTACCCATACTCCGAACTTCTTGGGATTGTACCAAAATTTGGGACTCTGGAAAGGGTCATATTATGGTGAAGGCATCATCATTGGAGTTTTGGATACTGGTATAACACCAGGACATCCCTCATTCCACGACGCAGGAGTTAACCCTCCTCCAGCTAAATGGAAAGGCAAGTGTGATTTCAATGGTACATTCTGTAACAATAAGCTTATTGGTGCCAGAAGTTTCCAAGGTGGGAAATCTACTGGTGCTCCACCAGTTGACATTGAAGGCCATGGAACCCACACGTCGAGCACAGCAGCAGGGAACTTTGTGAAAGGCGCCAACGTGTTTGGAATGGCAGAAGGAACAGCCTCCGGCATGGCACCTTATGCTCACGTGGCAATGTATCAAATCTGCAATGAAGATGGTTGTTCTGAAAGTGACATTGTAGCTGCTATGGATACTGCTGTTGCTGATGGAGTGGACGTGCTCTCCCTCTCACTCGGTGGTGGCTCAGTTCCTTTCTATGGTGATGGAATTGCAGTTGGTGCCTTTGGTGCAATTCAAAAGGGAATTTTTGTCAGCTGCTCAGCAGGAAACTCTGGTCCTGATTATGGTTCTTTATCCAATGAAGCCCCATGGATTCTAACCGTTGGAGCAAGCACCGTTGACAGAAGCATCAAGGCAACAGCAAAACTTGGAAATGGGAAAGAATATGATGGGGAATCATTATTCCAGGATAAAGATTTCAGTTCAGAGTTGTTACCTCTTGTTTATGCGGGTGCATATAGCAATGATTCAACAGCTCTATGTGATATTGGAACCCTTAATGGAGTTGAGGGGCAAGTTGTGCTGTGTGAAAGAGGTGGTGGAATTGGAAGAATTGCCAAAGGAGAAGAAGTCAAAAGAGCAGGTGGGGCTGCCATGATCCTCATGAACCAAGAGACTGATGGCTTTAGCACCTTAGCTGATGCTCATGTGCTTCCTGCAACACATTTGAGTTATGCTGCAGGTGTGAGTATCAAAGAGTACATAAACTCCACCTCAACACCTACAGCCACAATTTTGTTCAAAGGCACTGTCATCGGTGATCAAAATGCTCCCGCAGTCACTTCCTTTTCATCAAGAGGACCAAGCATTGCAAGCACAGGAATCTTGAAACCTGACATCATTGGTCCTGGTGTTAGCATTCTAGCTGCATGGCCTGTTTCAGTGGATAATGGAACAGAATCTACGGAAACATTTAACATGGTTTCAGGTACCTCAATGTCATGCCCTCACCTGAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCAATTGTGACAACCGCTGACATGGTAAACCTCGGAGGCAAGCCTATTGTTGATGAAACACTTACTCCAGCAGACATCTTTGCCACTGGTGCAGGACACGTTAATCCTACAAAAGCAGATGACCCCGGGCTAATCTACGACACAACACCAGGGGATTACATTCCCTACCTGTGCGGTTTGAACTACACAGAAGATCAAATACAGATAATCACCCAACAAACAGTGAAGTGCTCAGAAGTAGGAGTCATACCAGAACAGGAGCTAAATTATCCTTCCTTTTCTATTATATTCCAGGACTTGTCTGAGTCTCAATCTTACACAAGGACGGTGACAAATGTTGGACCAGCTAATTCAACTTACAACTTGGAACTCATGGTACCACATAAAATGGGCATGAGTGTGAACCCGGGACAACTTGTGTTTACAGAGGTTGGCCAGACAATGACATACAGAGTAGATTTCATCCCGGAAGAAGGTGCAGGGAAGGATGCACCATTTTCTCAGGGATATTTGAGATGGTTCAATGATCAGCATTCTGTTGCTAGCCCCATATCTGTGATCTTCTTCACCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

771

Amino Acids

81.66

Weight (kDa)

4.99

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 52 - 136 1e-08 Peptidase inhibitor I9
Peptidase_S8 PF00082 159 - 587 2.4e-46 Subtilase family
PA PF02225 385 - 469 7.6e-12 PA domain
fn3_6 PF17766 665 - 764 1.5e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1289, 1388
Acc36I ACCTGC 2 cut(s) 1289, 1388
Acc65I GGTACC 2 cut(s) 1646, 2110
AccB1I GGYRCC 6 cut(s) 620, 725, 769, 935, 1646, 2110
AccB7I CCANNNNNTGG 1 cut(s) 448
AciI CCGC 5 cut(s) 356, 1166, 1491, 1749, 1908
AclWI GGATC 1 cut(s) 1309
AcoI YGGCCR 1 cut(s) 2167
AcsI RAATTY 2 cut(s) 445, 960
AcvI CACGTG 1 cut(s) 784
AcyI GRCGYC 1 cut(s) 726
AdeI CACNNNGTG 2 cut(s) 304, 893
AfaI GTAC 6 cut(s) 227, 440, 595, 1418, 1648, 2112
AfiI CCNNNNNNNGG 4 cut(s) 448, 914, 1001, 2207
AflIII ACRYGT 3 cut(s) 690, 732, 1831
AgsI TTSAA 9 cut(s) 589, 674, 953, 1188, 1459, 1546, 1915, 2088, 2257
AjiI CACGTC 2 cut(s) 693, 874
AjnI CCWGG 5 cut(s) 517, 1122, 1564, 1882, 2025
AleI CACNNNNGTG 1 cut(s) 1671
Alw21I GWGCWC 5 cut(s) 658, 701, 879, 1700, 1967
Alw26I GTCTC 2 cut(s) 1325, 2046
AlwI GGATC 1 cut(s) 1309
Ama87I CYCGRG 2 cut(s) 1859, 2140
AoxI GGCC 3 cut(s) 676, 1589, 2167
ApeKI GCWGC 9 cut(s) 320, 704, 839, 972, 1307, 1394, 1583, 1682, 1721
ApoI RAATTY 2 cut(s) 445, 960
ArsI GACNNNNNNTTYG 2 cut(s) 1452, 1484
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 2 cut(s) 1646, 2110
AspLEI GCGC 1 cut(s) 728
AspS9I GGNCC 4 cut(s) 992, 1517, 1562, 2075
AsuC2I CCSGG 5 cut(s) 1860, 1861, 2141, 2142, 2201
AsuNHI GCTAGC 1 cut(s) 2276
AvaI CYCGRG 2 cut(s) 1859, 2140
AvaII GGWCC 4 cut(s) 992, 1517, 1562, 2075
BaeI ACNNNNGTAYC 2 cut(s) 840, 873
BalI TGGCCA 1 cut(s) 2169
BanI GGYRCC 6 cut(s) 620, 725, 769, 935, 1646, 2110
BanII GRGCYC 1 cut(s) 1700
BarI GAAGNNNNNNTAC 2 cut(s) 1917, 1949
BbrPI CACGTG 1 cut(s) 784
Bbv12I GWGCWC 5 cut(s) 658, 701, 879, 1700, 1967
BbvI GCAGC 9 cut(s) 332, 716, 826, 959, 1294, 1381, 1570, 1694, 1708
BcgI CGANNNNNNTGC 2 cut(s) 1453, 1487
BciT130I CCWGG 5 cut(s) 519, 1124, 1566, 1884, 2027
BciVI GTATCC 2 cut(s) 496, 841
BclI TGATCA 2 cut(s) 1477, 2260
BcnI CCSGG 5 cut(s) 1860, 1861, 2141, 2142, 2201
BcoDI GTCTC 2 cut(s) 1325, 2046
BfaI CTAG 3 cut(s) 74, 1580, 2277
BfmI CTRYAG 3 cut(s) 26, 1395, 1440
BfoI RGCGCY 1 cut(s) 729
BfuAI ACCTGC 2 cut(s) 1289, 1388
BfuI GTATCC 2 cut(s) 496, 841
BisI GCNGC 9 cut(s) 321, 705, 840, 973, 1308, 1395, 1584, 1683, 1722
BlpI GCTNAGC 1 cut(s) 976
BlsI GCNGC 9 cut(s) 322, 706, 841, 974, 1309, 1396, 1585, 1684, 1723
Bme18I GGWCC 4 cut(s) 992, 1517, 1562, 2075
BmeT110I CYCGRG 2 cut(s) 1859, 2140
BmgBI CACGTC 2 cut(s) 693, 874
BmgT120I GGNCC 4 cut(s) 992, 1517, 1562, 2075
BmiI GGNNCC 8 cut(s) 622, 685, 727, 771, 937, 1210, 1648, 2112
BmsI GCATC 4 cut(s) 492, 1074, 1348, 2212
BmtI GCTAGC 1 cut(s) 2280
BpmI CTGGAG 2 cut(s) 543, 1785
Bpu10I CCTNAGC 1 cut(s) 1351
Bpu1102I GCTNAGC 1 cut(s) 976
BpuEI CTTGAG 2 cut(s) 70, 1676
BpuMI CCSGG 5 cut(s) 1860, 1861, 2141, 2142, 2201
BsaAI YACGTR 1 cut(s) 784
BsaHI GRCGYC 1 cut(s) 726
BsaJI CCNNGG 8 cut(s) 634, 679, 1025, 1765, 1858, 1859, 1883, 2140
Bsc4I CCNNNNNNNGG 4 cut(s) 448, 914, 1001, 2207
Bse1I ACTGG 6 cut(s) 316, 511, 655, 662, 1715, 1825
Bse3DI GCAATG 6 cut(s) 401, 795, 811, 1186, 1524, 1677
BseBI CCWGG 5 cut(s) 519, 1124, 1566, 1884, 2027
BseDI CCNNGG 8 cut(s) 634, 679, 1025, 1765, 1858, 1859, 1883, 2140
BseGI GGATG 3 cut(s) 523, 2196, 2227
BseLI CCNNNNNNNGG 4 cut(s) 448, 914, 1001, 2207
BseMI GCAATG 6 cut(s) 401, 795, 811, 1186, 1524, 1677
BseMII CTCAG 6 cut(s) 915, 990, 1661, 1980, 2028, 2249
BseNI ACTGG 6 cut(s) 316, 511, 655, 662, 1715, 1825
BseRI GAGGAG 1 cut(s) 546
BseXI GCAGC 9 cut(s) 332, 716, 826, 959, 1294, 1381, 1570, 1694, 1708
BseYI CCCAGC 1 cut(s) 2304
BsgI GTGCAG 2 cut(s) 1845, 2232
BshFI GGCC 3 cut(s) 678, 1591, 2169
BshNI GGYRCC 6 cut(s) 620, 725, 769, 935, 1646, 2110
BsiHKAI GWGCWC 5 cut(s) 658, 701, 879, 1700, 1967
BsiHKCI CYCGRG 2 cut(s) 1859, 2140
BsiSI CCGG 4 cut(s) 762, 1860, 2141, 2201
BslFI GGGAC 3 cut(s) 187, 465, 2157
BslI CCNNNNNNNGG 4 cut(s) 448, 914, 1001, 2207
BsmAI GTCTC 2 cut(s) 1325, 2046
BsmFI GGGAC 3 cut(s) 187, 465, 2157
BsmI GAATGC 2 cut(s) 1575, 2266
BsnI GGCC 3 cut(s) 678, 1591, 2169
BsoBI CYCGRG 2 cut(s) 1859, 2140
Bsp1286I GDGCHC 5 cut(s) 658, 701, 879, 1700, 1967
Bsp143I GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
Bsp1720I GCTNAGC 1 cut(s) 976
Bsp19I CCATGG 2 cut(s) 679, 1025
BspACI CCGC 5 cut(s) 356, 1166, 1491, 1749, 1908
BspANI GGCC 3 cut(s) 678, 1591, 2169
BspCNI CTCAG 6 cut(s) 914, 989, 1662, 1979, 2029, 2248
BspHI TCATGA 1 cut(s) 1320
BspLI GGNNCC 8 cut(s) 622, 685, 727, 771, 937, 1210, 1648, 2112
BspMAI CTGCAG 2 cut(s) 30, 1399
BspMI ACCTGC 2 cut(s) 1289, 1388
BspOI GCTAGC 1 cut(s) 2280
BspPI GGATC 1 cut(s) 1309
BspT107I GGYRCC 6 cut(s) 620, 725, 769, 935, 1646, 2110
BsrDI GCAATG 6 cut(s) 401, 795, 811, 1186, 1524, 1677
BsrI ACTGG 6 cut(s) 316, 511, 655, 662, 1715, 1825
BssECI CCNNGG 8 cut(s) 634, 679, 1025, 1765, 1858, 1859, 1883, 2140
BssMI GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
BssNI GRCGYC 1 cut(s) 726
BssT1I CCWWGG 3 cut(s) 634, 679, 1025
Bst2UI CCWGG 5 cut(s) 519, 1124, 1566, 1884, 2027
Bst4CI ACNGT 5 cut(s) 1039, 1054, 1468, 1957, 2062
Bst6I CTCTTC 1 cut(s) 126
BstACI GRCGYC 1 cut(s) 726
BstAPI GCANNNNNTGC 3 cut(s) 776, 935, 1237
BstBAI YACGTR 1 cut(s) 784
BstC8I GCNNGC 5 cut(s) 149, 1048, 1531, 1775, 2278
BstDEI CTNAG 8 cut(s) 214, 901, 976, 1351, 1670, 1966, 2037, 2235
BstDSI CCRYGG 2 cut(s) 679, 1025
BstEII GGTNACC 1 cut(s) 1713
BstF5I GGATG 3 cut(s) 523, 2196, 2227
BstH2I RGCGCY 1 cut(s) 729
BstHHI GCGC 1 cut(s) 728
BstKTI GATC 6 cut(s) 33, 1317, 1480, 1930, 2263, 2295
BstMAI GTCTC 2 cut(s) 1325, 2046
BstMBI GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
BstMWI GCNNNNNNNGC 8 cut(s) 776, 785, 935, 944, 1237, 1304, 1530, 1823
BstNI CCWGG 5 cut(s) 519, 1124, 1566, 1884, 2027
BstNSI RCATGY 1 cut(s) 151
BstPI GGTNACC 1 cut(s) 1713
BstSFI CTRYAG 3 cut(s) 26, 1395, 1440
BstV1I GCAGC 9 cut(s) 332, 716, 826, 959, 1294, 1381, 1570, 1694, 1708
BstXI CCANNNNNNTGG 1 cut(s) 567
BsuI GTATCC 2 cut(s) 496, 841
BsuRI GGCC 3 cut(s) 678, 1591, 2169
BtgI CCRYGG 2 cut(s) 679, 1025
BtrI CACGTC 2 cut(s) 693, 874
BtsCI GGATG 3 cut(s) 523, 2196, 2227
BtsIMutI CAGTG 4 cut(s) 1464, 1605, 1818, 1962
BveI ACCTGC 2 cut(s) 1289, 1388
Cac8I GCNNGC 5 cut(s) 149, 1048, 1531, 1775, 2278
CciI TCATGA 1 cut(s) 1320
CfoI GCGC 1 cut(s) 728
Cfr13I GGNCC 4 cut(s) 992, 1517, 1562, 2075
Cfr9I CCCGGG 2 cut(s) 1859, 2140
CseI GACGC 1 cut(s) 548
Csp6I GTAC 6 cut(s) 226, 439, 594, 1417, 1647, 2111
CviQI GTAC 6 cut(s) 226, 439, 594, 1417, 1647, 2111
DdeI CTNAG 8 cut(s) 214, 901, 976, 1351, 1670, 1966, 2037, 2235
DinI GGCGCC 1 cut(s) 727
DpnI GATC 6 cut(s) 32, 1316, 1479, 1929, 2262, 2294
DpnII GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
DraIII CACNNNGTG 2 cut(s) 304, 893
EaeI YGGCCR 1 cut(s) 2167
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 1698
Eco130I CCWWGG 3 cut(s) 634, 679, 1025
Eco24I GRGCYC 1 cut(s) 1700
Eco47I GGWCC 4 cut(s) 992, 1517, 1562, 2075
Eco53kI GAGCTC 1 cut(s) 1698
Eco72I CACGTG 1 cut(s) 784
Eco88I CYCGRG 2 cut(s) 1859, 2140
Eco91I GGTNACC 1 cut(s) 1713
EcoICRI GAGCTC 1 cut(s) 1698
EcoO65I GGTNACC 1 cut(s) 1713
EcoRII CCWGG 5 cut(s) 517, 1122, 1564, 1882, 2025
EcoT14I CCWWGG 3 cut(s) 634, 679, 1025
EcoT38I GRGCYC 1 cut(s) 1700
EgeI GGCGCC 1 cut(s) 727
EheI GGCGCC 1 cut(s) 727
ErhI CCWWGG 3 cut(s) 634, 679, 1025
FaqI GGGAC 3 cut(s) 187, 465, 2157
FauI CCCGC 2 cut(s) 1159, 1498
FbaI TGATCA 2 cut(s) 1477, 2260
Fnu4HI GCNGC 9 cut(s) 321, 705, 840, 973, 1308, 1395, 1584, 1683, 1722
FokI GGATG 3 cut(s) 510, 2183, 2234
FriOI GRGCYC 1 cut(s) 1700
Fsp4HI GCNGC 9 cut(s) 321, 705, 840, 973, 1308, 1395, 1584, 1683, 1722
FspBI CTAG 3 cut(s) 74, 1580, 2277
GlaI GCGC 1 cut(s) 727
GluI GCNGC 9 cut(s) 321, 705, 840, 973, 1308, 1395, 1584, 1683, 1722
GsaI CCCAGC 1 cut(s) 2308
GsuI CTGGAG 2 cut(s) 543, 1785
HaeII RGCGCY 1 cut(s) 729
HaeIII GGCC 3 cut(s) 678, 1591, 2169
HapII CCGG 4 cut(s) 762, 1860, 2141, 2201
HgaI GACGC 1 cut(s) 548
HhaI GCGC 1 cut(s) 728
Hin1I GRCGYC 1 cut(s) 726
Hin6I GCGC 1 cut(s) 726
HinP1I GCGC 1 cut(s) 726
HincII GTYRAC 3 cut(s) 550, 667, 1057
HindII GTYRAC 3 cut(s) 550, 667, 1057
HindIII AAGCTT 1 cut(s) 611
HpaI GTTAAC 1 cut(s) 550
HpaII CCGG 4 cut(s) 762, 1860, 2141, 2201
Hpy166II GTNNAC 8 cut(s) 289, 550, 667, 871, 1057, 1762, 2137, 2157
Hpy188I TCNGA 7 cut(s) 199, 423, 823, 1144, 1769, 1969, 2038
Hpy188III TCNNGA 8 cut(s) 209, 386, 457, 995, 1321, 1512, 1543, 1693
Hpy8I GTNNAC 8 cut(s) 289, 550, 667, 871, 1057, 1762, 2137, 2157
Hpy99I CGWCG 2 cut(s) 542, 697
HpyAV CCTTC 7 cut(s) 70, 473, 668, 743, 2016, 2201, 2212
HpyCH4III ACNGT 5 cut(s) 1039, 1054, 1468, 1957, 2062
HpyCH4IV ACGT 5 cut(s) 692, 732, 783, 873, 1833
HpyF10VI GCNNNNNNNGC 8 cut(s) 776, 785, 935, 944, 1237, 1304, 1530, 1823
HpyF3I CTNAG 8 cut(s) 214, 901, 976, 1351, 1670, 1966, 2037, 2235
HpySE526I ACGT 5 cut(s) 692, 732, 783, 873, 1833
Hsp92I GRCGYC 1 cut(s) 726
HspAI GCGC 1 cut(s) 726
KasI GGCGCC 1 cut(s) 725
KpnI GGTACC 2 cut(s) 1650, 2114
Ksp22I TGATCA 2 cut(s) 1477, 2260
KspAI GTTAAC 1 cut(s) 550
Kzo9I GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
LmnI GCTCC 4 cut(s) 661, 1043, 1492, 1993
Lsp1109I GCAGC 9 cut(s) 332, 716, 826, 959, 1294, 1381, 1570, 1694, 1708
LweI GCATC 4 cut(s) 492, 1074, 1348, 2212
MaeI CTAG 3 cut(s) 74, 1580, 2277
MaeII ACGT 5 cut(s) 692, 732, 783, 873, 1833
MaeIII GTNAC 7 cut(s) 602, 827, 1149, 1495, 1713, 1741, 2062
MalI GATC 6 cut(s) 32, 1316, 1479, 1929, 2262, 2294
MboI GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
MboII GAAGA 9 cut(s) 32, 143, 821, 1278, 1295, 1937, 2216, 2287, 2290
MfeI CAATTG 1 cut(s) 1737
MhlI GDGCHC 5 cut(s) 658, 701, 879, 1700, 1967
MlsI TGGCCA 1 cut(s) 2169
MluNI TGGCCA 1 cut(s) 2169
Mly113I GGCGCC 1 cut(s) 726
MlyI GAGTC 4 cut(s) 209, 447, 1986, 2048
MmeI TCCRAC 2 cut(s) 1021, 2053
Mox20I TGGCCA 1 cut(s) 2169
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 2169
MseI TTAA 5 cut(s) 549, 1215, 1632, 1728, 1836
MslI CAYNNNNRTG 3 cut(s) 1671, 2132, 2288
Msp20I TGGCCA 1 cut(s) 2169
MspA1I CMGCKG 3 cut(s) 972, 1721, 1751
MspI CCGG 4 cut(s) 762, 1860, 2141, 2201
MunI CAATTG 1 cut(s) 1737
Mva1269I GAATGC 2 cut(s) 1575, 2266
MvaI CCWGG 5 cut(s) 519, 1124, 1566, 1884, 2027
MwoI GCNNNNNNNGC 8 cut(s) 776, 785, 935, 944, 1237, 1304, 1530, 1823
NarI GGCGCC 1 cut(s) 726
NciI CCSGG 5 cut(s) 1860, 1861, 2141, 2142, 2201
NcoI CCATGG 2 cut(s) 679, 1025
NdeII GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
NheI GCTAGC 1 cut(s) 2276
NlaIV GGNNCC 8 cut(s) 622, 685, 727, 771, 937, 1210, 1648, 2112
NmuCI GTSAC 5 cut(s) 827, 1495, 1713, 1741, 2062
NspI RCATGY 1 cut(s) 151
OliI CACNNNNGTG 1 cut(s) 1671
PaeI GCATGC 1 cut(s) 151
PagI TCATGA 1 cut(s) 1320
PaqCI CACCTGC 2 cut(s) 1289, 1388
PctI GAATGC 2 cut(s) 1575, 2266
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 7 cut(s) 119, 394, 1030, 1112, 1184, 1539, 1616
PflMI CCANNNNNTGG 1 cut(s) 448
PfoI TCCNGGA 3 cut(s) 1122, 2025, 2199
PkrI GCNGC 9 cut(s) 322, 706, 841, 974, 1309, 1396, 1585, 1684, 1723
PleI GAGTC 4 cut(s) 208, 447, 1985, 2047
PluTI GGCGCC 1 cut(s) 729
PmaCI CACGTG 1 cut(s) 784
PmlI CACGTG 1 cut(s) 784
PpsI GAGTC 4 cut(s) 208, 447, 1985, 2047
Ppu21I YACGTR 1 cut(s) 784
Psp124BI GAGCTC 1 cut(s) 1700
Psp6I CCWGG 5 cut(s) 517, 1122, 1564, 1882, 2025
PspCI CACGTG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 1713
PspFI CCCAGC 1 cut(s) 2304
PspGI CCWGG 5 cut(s) 517, 1122, 1564, 1882, 2025
PspN4I GGNNCC 8 cut(s) 622, 685, 727, 771, 937, 1210, 1648, 2112
PspPI GGNCC 4 cut(s) 992, 1517, 1562, 2075
PsrI GAACNNNNNNTAC 4 cut(s) 1603, 1635, 2094, 2126
PstI CTGCAG 2 cut(s) 30, 1399
PvuII CAGCTG 2 cut(s) 972, 1721
RsaI GTAC 6 cut(s) 227, 440, 595, 1418, 1648, 2112
RsaNI GTAC 6 cut(s) 226, 439, 594, 1417, 1647, 2111
RseI CAYNNNNRTG 3 cut(s) 1671, 2132, 2288
SacI GAGCTC 1 cut(s) 1700
SaqAI TTAA 5 cut(s) 549, 1215, 1632, 1728, 1836
SatI GCNGC 9 cut(s) 321, 705, 840, 973, 1308, 1395, 1584, 1683, 1722
Sau3AI GATC 6 cut(s) 30, 1314, 1477, 1927, 2260, 2292
Sau96I GGNCC 4 cut(s) 992, 1517, 1562, 2075
SchI GAGTC 4 cut(s) 209, 447, 1986, 2048
SduI GDGCHC 5 cut(s) 658, 701, 879, 1700, 1967
SfaNI GCATC 4 cut(s) 492, 1074, 1348, 2212
SfcI CTRYAG 3 cut(s) 26, 1395, 1440
SfoI GGCGCC 1 cut(s) 727
SinI GGWCC 4 cut(s) 992, 1517, 1562, 2075
SmaI CCCGGG 2 cut(s) 1861, 2142
SmiMI CAYNNNNRTG 3 cut(s) 1671, 2132, 2288
SmlI CTYRAG 2 cut(s) 49, 1691
SmoI CTYRAG 2 cut(s) 49, 1691
SphI GCATGC 1 cut(s) 151
SsiI CCGC 5 cut(s) 356, 1166, 1491, 1749, 1908
SspDI GGCGCC 1 cut(s) 725
SspMI CTAG 3 cut(s) 74, 1580, 2277
SstI GAGCTC 1 cut(s) 1700
StyI CCWWGG 3 cut(s) 634, 679, 1025
TaaI ACNGT 5 cut(s) 1039, 1054, 1468, 1957, 2062
TaiI ACGT 5 cut(s) 695, 735, 786, 876, 1836
TaqI TCGA 1 cut(s) 695
TatI WGTACW 1 cut(s) 1416
TfiI GAWTC 7 cut(s) 119, 394, 1030, 1112, 1184, 1539, 1616
Tru1I TTAA 5 cut(s) 549, 1215, 1632, 1728, 1836
Tru9I TTAA 5 cut(s) 549, 1215, 1632, 1728, 1836
TscAI CASTG 4 cut(s) 1471, 1605, 1825, 1962
TseFI GTSAC 5 cut(s) 827, 1495, 1713, 1741, 2062
TseI GCWGC 9 cut(s) 320, 704, 839, 972, 1307, 1394, 1583, 1682, 1721
Tsp45I GTSAC 5 cut(s) 827, 1495, 1713, 1741, 2062
TspDTI ATGAA 6 cut(s) 822, 1032, 1337, 1497, 1803, 2185
TspGWI ACGGA 1 cut(s) 1637
TspMI CCCGGG 2 cut(s) 1859, 2140
TspRI CASTG 4 cut(s) 1471, 1605, 1825, 1962
Van91I CCANNNNNTGG 1 cut(s) 448
VpaK11BI GGWCC 4 cut(s) 992, 1517, 1562, 2075
XapI RAATTY 2 cut(s) 445, 960
XceI RCATGY 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 2 cut(s) 308, 1334
XmaI CCCGGG 2 cut(s) 1859, 2140
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 3 cut(s) 74, 1580, 2277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.