RLG00000003743

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000302
Physical Location & Seq
Forward (+)
52230425 .. 52231727
1303 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003743

Sequence Viewer

Length: 975 bp
ATGGAAAAGAAAGACGGGTTCGTGCATGCTCATCCAGAGAGGCTTCTGCCTTTGCAAACTACTCATAGTCCCAACTTCTTGGGGCTGCAACAAGGGTTAGGACTTTGGAAAGGGACTAATTACCGGGAAGGTGTGATCATTGGAGTTTTGGATACTGGAATATCTCCAGATCATCCCTCATTTAGAGATGATGGAGTGCCACCTCCCCCGGCGAAATGGAAAGGCAAGTGCGAGTTCAATGGGGCAGTGTGTAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGAAGGCCATGGCACTCACACCTCCAGCACAGCTGCCGGAAACTTTGGGAAAGGTGCCAGCGTGTTTGGAATGGCAAGTGGCACAGCCTCTGGCATTGCACCTTATGCTCACTTGGCCATCTACAAGGTCTGCACCGAGGCAGGTTGTGCCGAAAGTGACATGCTAGCAGCTCTTGACACTGCTGTTGAGGACGGCGTGGATGTGCTCTTACTTTCCATAGGTGGTTCCTCAATTCCTTTCCATCAAGATGGGATTGCTATCAGCGCCTTTGGACCAATTCAAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAACTCTGGCCCTTTCTATAGTTCCACGTCGAATGAGGCCCCTTGGATTCTCACCGTGGGAGCAAGCACCATTGACAGAAAAATAAGCTCATCAGCACAGCTTGGAGATTCTCCAAAATATTTTTGCTCCGGTGAATCTTTATTCGATCCTAAAAATTTTAGCACTTCACCATTGCTACCTCTTGTTTATCCAGGTGCAAATCCGAATAACGAAACTGAGTTCTGTGCCTCGGGATCTCTTGTAAATGTTGAAGGAGGACCGAGCATAGACAGCCCCGGAATTTTGAAACCTGACTTCATTGGTCCTGGTGTTAACATCCTCGCTGCATGGCCTGTTTCGGTGGATAATGTCACAACATTTAACATAATTTTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

325

Amino Acids

33.83

Weight (kDa)

5.31

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 43 - 265 2.2e-24 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 338
AclWI GGATC 2 cut(s) 737, 838
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 4 cut(s) 277, 573, 751, 876
AgsI TTSAA 4 cut(s) 238, 566, 848, 883
AjiI CACGTC 1 cut(s) 624
AjnI CCWGG 2 cut(s) 787, 901
AluBI AGCT 7 cut(s) 262, 317, 455, 585, 593, 684, 697
AluI AGCT 7 cut(s) 262, 317, 455, 585, 593, 684, 697
Alw21I GWGCWC 1 cut(s) 492
AlwI GGATC 2 cut(s) 737, 838
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 826
AoxI GGCC 5 cut(s) 289, 399, 604, 633, 926
ApeKI GCWGC 4 cut(s) 85, 317, 452, 920
ApoI RAATTY 4 cut(s) 277, 573, 751, 876
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 5 cut(s) 557, 605, 634, 854, 899
AsuC2I CCSGG 3 cut(s) 125, 209, 873
AsuHPI GGTGA 3 cut(s) 640, 740, 756
AsuNHI GCTAGC 1 cut(s) 448
AvaI CYCGRG 1 cut(s) 826
AvaII GGWCC 3 cut(s) 557, 854, 899
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 4 cut(s) 72, 304, 464, 907
BccI CCATC 4 cut(s) 185, 410, 527, 534
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 2 cut(s) 789, 903
BciVI GTATCC 1 cut(s) 145
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 3 cut(s) 125, 209, 873
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 613
BfoI RGCGCY 1 cut(s) 552
BfuAI ACCTGC 1 cut(s) 416
BfuI GTATCC 1 cut(s) 145
BisI GCNGC 4 cut(s) 86, 318, 453, 921
BlsI GCNGC 4 cut(s) 87, 319, 454, 922
Bme1390I CCNGG 5 cut(s) 125, 209, 789, 873, 903
Bme18I GGWCC 3 cut(s) 557, 854, 899
BmeT110I CYCGRG 1 cut(s) 826
BmgBI CACGTC 1 cut(s) 624
BmgT120I GGNCC 5 cut(s) 557, 605, 634, 854, 899
BmiI GGNNCC 3 cut(s) 340, 511, 636
BmrFI CCNGG 5 cut(s) 125, 209, 789, 873, 903
BmtI GCTAGC 1 cut(s) 452
BpmI CTGGAG 2 cut(s) 150, 292
BpuMI CCSGG 3 cut(s) 125, 209, 873
BsaBI GATNNNNATC 1 cut(s) 542
BsaJI CCNNGG 7 cut(s) 207, 292, 420, 638, 651, 825, 871
BsaWI WCCGGW 1 cut(s) 725
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 2 cut(s) 378, 767
Bse8I GATNNNNATC 1 cut(s) 542
BseBI CCWGG 2 cut(s) 789, 903
BseDI CCNNGG 7 cut(s) 207, 292, 420, 638, 651, 825, 871
BseGI GGATG 4 cut(s) 31, 172, 490, 912
BseJI GATNNNNATC 1 cut(s) 542
BseMI GCAATG 2 cut(s) 378, 767
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 160
BseXI GCAGC 4 cut(s) 72, 304, 464, 907
BsgI GTGCAG 1 cut(s) 400
BshFI GGCC 5 cut(s) 291, 401, 606, 635, 928
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 1 cut(s) 826
BsiSI CCGG 5 cut(s) 124, 209, 321, 726, 873
BslFI GGGAC 2 cut(s) 54, 127
BsmFI GGGAC 2 cut(s) 54, 127
BsnI GGCC 5 cut(s) 291, 401, 606, 635, 928
BsoBI CYCGRG 1 cut(s) 826
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 4 cut(s) 135, 169, 742, 830
Bsp19I CCATGG 1 cut(s) 292
BspANI GGCC 5 cut(s) 291, 401, 606, 635, 928
BspCNI CTCAG 1 cut(s) 805
BspLI GGNNCC 3 cut(s) 340, 511, 636
BspMI ACCTGC 1 cut(s) 416
BspOI GCTAGC 1 cut(s) 452
BspPI GGATC 2 cut(s) 737, 838
BspT107I GGYRCC 1 cut(s) 338
BsrDI GCAATG 2 cut(s) 378, 767
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 7 cut(s) 207, 292, 420, 638, 651, 825, 871
BssMI GATC 4 cut(s) 135, 169, 742, 830
BssT1I CCWWGG 2 cut(s) 292, 638
Bst2UI CCWGG 2 cut(s) 789, 903
Bst4CI ACNGT 1 cut(s) 652
BstAPI GCANNNNNTGC 2 cut(s) 389, 431
BstC8I GCNNGC 4 cut(s) 27, 343, 450, 661
BstDEI CTNAG 1 cut(s) 813
BstDSI CCRYGG 2 cut(s) 292, 651
BstF5I GGATG 4 cut(s) 31, 172, 490, 912
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 4 cut(s) 138, 172, 745, 833
BstMBI GATC 4 cut(s) 135, 169, 742, 830
BstMWI GCNNNNNNNGC 5 cut(s) 389, 398, 431, 548, 867
BstNI CCWGG 2 cut(s) 789, 903
BstNSI RCATGY 2 cut(s) 29, 448
BstSCI CCNGG 5 cut(s) 123, 207, 787, 871, 901
BstSFI CTRYAG 1 cut(s) 613
BstV1I GCAGC 4 cut(s) 72, 304, 464, 907
BstX2I RGATCY 1 cut(s) 830
BstXI CCANNNNNNTGG 2 cut(s) 79, 533
BstYI RGATCY 1 cut(s) 830
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 5 cut(s) 291, 401, 606, 635, 928
BtgI CCRYGG 2 cut(s) 292, 651
BtrI CACGTC 1 cut(s) 624
BtsCI GGATG 4 cut(s) 31, 172, 490, 912
BtsI GCAGTG 2 cut(s) 252, 462
BtsIMutI CAGTG 2 cut(s) 252, 462
BveI ACCTGC 1 cut(s) 416
Cac8I GCNNGC 4 cut(s) 27, 343, 450, 661
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 5 cut(s) 557, 605, 634, 854, 899
CviAII CATG 4 cut(s) 26, 293, 445, 924
DdeI CTNAG 1 cut(s) 813
DpnI GATC 4 cut(s) 137, 171, 744, 832
DpnII GATC 4 cut(s) 135, 169, 742, 830
EaeI YGGCCR 1 cut(s) 399
Eco130I CCWWGG 2 cut(s) 292, 638
Eco47I GGWCC 3 cut(s) 557, 854, 899
Eco88I CYCGRG 1 cut(s) 826
EcoO109I RGGNCCY 1 cut(s) 634
EcoRII CCWGG 2 cut(s) 787, 901
EcoT14I CCWWGG 2 cut(s) 292, 638
ErhI CCWWGG 2 cut(s) 292, 638
FaeI CATG 4 cut(s) 29, 296, 448, 927
FaqI GGGAC 2 cut(s) 54, 127
FatI CATG 4 cut(s) 25, 292, 444, 923
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 86, 318, 453, 921
FokI GGATG 4 cut(s) 18, 159, 497, 899
Fsp4HI GCNGC 4 cut(s) 86, 318, 453, 921
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 550
GluI GCNGC 4 cut(s) 86, 318, 453, 921
GsuI CTGGAG 2 cut(s) 150, 292
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 5 cut(s) 291, 401, 606, 635, 928
HapII CCGG 5 cut(s) 124, 209, 321, 726, 873
HhaI GCGC 1 cut(s) 551
Hin1II CATG 4 cut(s) 29, 296, 448, 927
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 3 cut(s) 643, 704, 731
HpaI GTTAAC 1 cut(s) 910
HpaII CCGG 5 cut(s) 124, 209, 321, 726, 873
HphI GGTGA 3 cut(s) 640, 740, 756
Hpy166II GTNNAC 1 cut(s) 910
Hpy188I TCNGA 1 cut(s) 801
Hpy188III TCNNGA 5 cut(s) 35, 167, 458, 530, 828
Hpy8I GTNNAC 1 cut(s) 910
Hpy99I CGWCG 1 cut(s) 628
HpyAV CCTTC 3 cut(s) 122, 281, 842
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 1 cut(s) 623
HpyCH4V TGCA 8 cut(s) 25, 55, 88, 272, 383, 417, 794, 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 389, 398, 431, 548, 867
HpyF3I CTNAG 1 cut(s) 813
HpySE526I ACGT 1 cut(s) 623
Hsp92II CATG 4 cut(s) 29, 296, 448, 927
HspAI GCGC 1 cut(s) 549
Ksp22I TGATCA 1 cut(s) 135
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 4 cut(s) 135, 169, 742, 830
LmnI GCTCC 2 cut(s) 656, 728
Lsp1109I GCAGC 4 cut(s) 72, 304, 464, 907
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 623
MaeIII GTNAC 3 cut(s) 251, 440, 946
MalI GATC 4 cut(s) 137, 171, 744, 832
MboI GATC 4 cut(s) 135, 169, 742, 830
MflI RGATCY 1 cut(s) 830
MhlI GDGCHC 1 cut(s) 492
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 8 cut(s) 118, 277, 516, 561, 573, 751, 876, 963
MluNI TGGCCA 1 cut(s) 401
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
MseI TTAA 2 cut(s) 909, 957
MslI CAYNNNNRTG 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 3 cut(s) 317, 585, 593
MspI CCGG 5 cut(s) 124, 209, 321, 726, 873
MspR9I CCNGG 5 cut(s) 125, 209, 789, 873, 903
MvaI CCWGG 2 cut(s) 789, 903
MwoI GCNNNNNNNGC 5 cut(s) 389, 398, 431, 548, 867
NciI CCSGG 3 cut(s) 125, 209, 873
NcoI CCATGG 1 cut(s) 292
NdeII GATC 4 cut(s) 135, 169, 742, 830
NheI GCTAGC 1 cut(s) 448
NlaIII CATG 4 cut(s) 29, 296, 448, 927
NlaIV GGNNCC 3 cut(s) 340, 511, 636
NmuCI GTSAC 2 cut(s) 440, 946
NspI RCATGY 2 cut(s) 29, 448
PaeI GCATGC 1 cut(s) 29
PfeI GAWTC 3 cut(s) 643, 704, 731
PkrI GCNGC 4 cut(s) 87, 319, 454, 922
Psp6I CCWGG 2 cut(s) 787, 901
PspGI CCWGG 2 cut(s) 787, 901
PspN4I GGNNCC 3 cut(s) 340, 511, 636
PspPI GGNCC 5 cut(s) 557, 605, 634, 854, 899
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 830
PvuII CAGCTG 3 cut(s) 317, 585, 593
RseI CAYNNNNRTG 1 cut(s) 531
SaqAI TTAA 2 cut(s) 909, 957
SatI GCNGC 4 cut(s) 86, 318, 453, 921
Sau3AI GATC 4 cut(s) 135, 169, 742, 830
Sau96I GGNCC 5 cut(s) 557, 605, 634, 854, 899
ScrFI CCNGG 5 cut(s) 125, 209, 789, 873, 903
SduI GDGCHC 1 cut(s) 492
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 3 cut(s) 557, 854, 899
SmiMI CAYNNNNRTG 1 cut(s) 531
SphI GCATGC 1 cut(s) 29
Sse9I AATT 8 cut(s) 118, 277, 516, 561, 573, 751, 876, 963
SspI AATATT 1 cut(s) 716
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 5 cut(s) 123, 207, 787, 871, 901
StyI CCWWGG 2 cut(s) 292, 638
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 1 cut(s) 626
TaqI TCGA 2 cut(s) 626, 741
TaqII GACCGA 1 cut(s) 871
TasI AATT 8 cut(s) 118, 277, 516, 561, 573, 751, 876, 963
TfiI GAWTC 3 cut(s) 643, 704, 731
Tru1I TTAA 2 cut(s) 909, 957
Tru9I TTAA 2 cut(s) 909, 957
TscAI CASTG 2 cut(s) 252, 469
TseFI GTSAC 2 cut(s) 440, 946
TseI GCWGC 4 cut(s) 85, 317, 452, 920
Tsp45I GTSAC 2 cut(s) 440, 946
TspDTI ATGAA 1 cut(s) 883
TspRI CASTG 2 cut(s) 252, 469
VpaK11BI GGWCC 3 cut(s) 557, 854, 899
XapI RAATTY 4 cut(s) 277, 573, 751, 876
XceI RCATGY 2 cut(s) 29, 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.