Rh7DG186300

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000302
Physical Location & Seq
Reverse (-)
17308425 .. 17308976
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG186300.1

Sequence Viewer

Length: 552 bp
ATGACTTCTGCTGATCTTCTAAACTTGCAAGGCAAACGGATTTTTGATGAAACACTTCAGACTGCAGATATCTTAGCCACCGGTGCGGGCCATGTCAATCCATCAAAGGCAACCGACCCAGGACTGATATATGATATTCAACCTGATGATTATATTCCATATCTTTGTGGCTTGGGCTACAACGATACTGGAGTCAGTATCCTCGTGCACAGACCAATAAAGTGCTCAAAGGTATCAAGCATCCCTGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCAAGCTGGGACCATCTCAGACATTCACAAGAACTGTGACAAATGTTGGTGCCTCATATTCCACTTATTCAGTCAAGGTATATGCACCACTAGGAACCCATGTGACTGTCAAACCCAGCACGCTTTACTTTACAGAGGTGAACCAGAAGGCGTCATATTCAGTGACATTCAGTCGTACTTCAGGAGGTAAAGCTGGTGCATTCACACAGGGATTTATAACATGGGCTTCTACTAATCACGTTGTCCGGAGTCCAGTTTCTGTTTTATTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

183

Amino Acids

19.72

Weight (kDa)

8.49

Isoelectric Point (pI)

25.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 85 - 180 8.9e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 326
AccIII TCCGGA 1 cut(s) 522
AciI CCGC 1 cut(s) 86
AcuI CTGAAG 3 cut(s) 41, 267, 441
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 1 cut(s) 454
AgeI ACCGGT 1 cut(s) 80
AgsI TTSAA 1 cut(s) 140
AhdI GACNNNNNGTC 1 cut(s) 447
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 3 cut(s) 256, 283, 470
AluI AGCT 3 cut(s) 256, 283, 470
Alw21I GWGCWC 2 cut(s) 210, 227
Alw44I GTGCAC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 522
AoxI GGCC 1 cut(s) 88
ApaLI GTGCAC 1 cut(s) 206
AsiGI ACCGGT 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 54
AspS9I GGNCC 2 cut(s) 88, 287
AsuHPI GGTGA 1 cut(s) 427
AvaII GGWCC 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 210
BaeI ACNNNNGTAYC 2 cut(s) 177, 210
BanI GGYRCC 1 cut(s) 326
BauI CACGAG 1 cut(s) 203
Bbv12I GWGCWC 2 cut(s) 210, 227
BccI CCATC 2 cut(s) 109, 298
BciT130I CCWGG 1 cut(s) 120
BciVI GTATCC 1 cut(s) 209
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 63
BfuI GTATCC 1 cut(s) 209
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 1 cut(s) 287
BmeRI GACNNNNNGTC 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 88, 287
BmiI GGNNCC 3 cut(s) 288, 328, 373
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 118
BsaWI WCCGGW 2 cut(s) 80, 522
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 193, 530
BseAI TCCGGA 1 cut(s) 522
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 193, 530
BseSI GKGCMC 1 cut(s) 210
BseYI CCCAGC 2 cut(s) 283, 392
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 326
BshTI ACCGGT 1 cut(s) 80
BsiHKAI GWGCWC 2 cut(s) 210, 227
BsiSI CCGG 2 cut(s) 81, 523
BslFI GGGAC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 300
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 1 cut(s) 90
Bsp1286I GDGCHC 2 cut(s) 210, 227
Bsp13I TCCGGA 1 cut(s) 522
Bsp143I GATC 1 cut(s) 13
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 1 cut(s) 307
BspEI TCCGGA 1 cut(s) 522
BspLI GGNNCC 3 cut(s) 288, 328, 373
BspMAI CTGCAG 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 2 cut(s) 193, 530
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 1 cut(s) 13
BssNI GRCGYC 1 cut(s) 428
BssSI CACGAG 1 cut(s) 203
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 2 cut(s) 313, 385
BstACI GRCGYC 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 88, 398
BstDEI CTNAG 2 cut(s) 73, 294
BstF5I GGATG 1 cut(s) 240
BstKTI GATC 1 cut(s) 16
BstMBI GATC 1 cut(s) 13
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 210
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 1 cut(s) 90
BtsCI GGATG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 88, 398
CaiI CAGNNNCTG 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 88, 287
CseI GACGC 1 cut(s) 417
Csp6I GTAC 1 cut(s) 453
CspAI ACCGGT 1 cut(s) 80
CviAII CATG 3 cut(s) 92, 377, 498
CviJI RGCY 8 cut(s) 77, 90, 171, 177, 256, 283, 470, 503
CviKI_1 RGCY 8 cut(s) 77, 90, 171, 177, 256, 283, 470, 503
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 2 cut(s) 73, 294
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
DriI GACNNNNNGTC 1 cut(s) 447
Eam1105I GACNNNNNGTC 1 cut(s) 447
Eco32I GATATC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 3 cut(s) 41, 267, 441
EcoRII CCWGG 1 cut(s) 118
EcoRV GATATC 1 cut(s) 70
FaeI CATG 3 cut(s) 95, 380, 501
FaqI GGGAC 1 cut(s) 300
FatI CATG 3 cut(s) 91, 376, 497
FauI CCCGC 1 cut(s) 79
FokI GGATG 1 cut(s) 227
FspBI CTAG 1 cut(s) 368
GsaI CCCAGC 2 cut(s) 287, 396
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 90
HapII CCGG 2 cut(s) 81, 523
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 3 cut(s) 95, 380, 501
HinfI GANTC 2 cut(s) 192, 526
HpaII CCGG 2 cut(s) 81, 523
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 2 cut(s) 208, 418
Hpy188I TCNGA 2 cut(s) 60, 297
Hpy188III TCNNGA 2 cut(s) 459, 523
Hpy8I GTNNAC 2 cut(s) 208, 418
HpyAV CCTTC 3 cut(s) 242, 276, 418
HpyCH4III ACNGT 2 cut(s) 313, 385
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 5 cut(s) 28, 65, 208, 362, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 73, 294
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 3 cut(s) 95, 380, 501
Kpn2I TCCGGA 1 cut(s) 522
Kzo9I GATC 1 cut(s) 13
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 3 cut(s) 313, 379, 439
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 210, 227
MlyI GAGTC 2 cut(s) 201, 535
MnlI CCTC 4 cut(s) 212, 340, 406, 455
MroI TCCGGA 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 54
MspI CCGG 2 cut(s) 81, 523
MspR9I CCNGG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 1 cut(s) 13
NlaIII CATG 3 cut(s) 95, 380, 501
NlaIV GGNNCC 3 cut(s) 288, 328, 373
NmuCI GTSAC 3 cut(s) 313, 379, 439
PctI GAATGC 1 cut(s) 476
PdmI GAANNNNTTC 1 cut(s) 54
PinAI ACCGGT 1 cut(s) 80
PleI GAGTC 2 cut(s) 200, 534
PpsI GAGTC 2 cut(s) 200, 534
PsiI TTATAA 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 118
PspFI CCCAGC 2 cut(s) 283, 392
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 3 cut(s) 288, 328, 373
PspPI GGNCC 2 cut(s) 88, 287
PstI CTGCAG 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 536
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
Sau3AI GATC 1 cut(s) 13
Sau96I GGNCC 2 cut(s) 88, 287
SchI GAGTC 2 cut(s) 201, 535
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 2 cut(s) 210, 227
SetI ASST 9 cut(s) 145, 234, 258, 285, 357, 417, 466, 472, 519
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 63
SgrAI CRCCGGYG 1 cut(s) 80
SinI GGWCC 1 cut(s) 287
SsiI CCGC 1 cut(s) 86
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 313, 385
TaiI ACGT 1 cut(s) 519
TscAI CASTG 1 cut(s) 444
TseFI GTSAC 3 cut(s) 313, 379, 439
Tsp45I GTSAC 3 cut(s) 313, 379, 439
TspDTI ATGAA 2 cut(s) 63, 258
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 1 cut(s) 444
VneI GTGCAC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.