Rroxscaffold_4G00311060

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000302
Physical Location & Seq
Forward (+)
33300579 .. 33302789
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311060.1

Sequence Viewer

Length: 1971 bp
ATGGTTCACTCATACCTCAATGTGGCCACCGGCTTTGCGGCAAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGAGAGGTTTCTGCCTTTGCACACTACTCATAGTCCAAACTTCCTGGGGCTGCAACAAGGGTTCGGACTTTGGAAAAGGACTAATTACGGTGAAGGCAAATGCGAGTTCAAGTTCAATCGGACAACAGTGTGCAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGGAAAGAAACAAAAGAAGTCTCTCCATTTGGTGAAGAAGGCCATGGCACTCTCACTTCTAGCATAGCTGCTGGAAACTTTGTGAAAGGCGCCAGCGTGTTTGGAATGGCAAATGGCACAGCATCTGGCATTGCACCTTATGCTCACGTGGCCATCTACAAGGTCTGCACCGAGGCAGGTTGTGCCGAAAGTGACATTCTAGCAGCTCTTGACACTGCTATTGAGGACGGCGTGGATGTGCTCTCACTGTCCATAGGTTTTGCTTCCTCAGTTCCTTTCCATCAAGATGGGGTTGCTATCGGCGCGTTTGGAGCAATTCAAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAACTCTGGCCCTTTCTATAGTTCCATGTCAAACGAGGCCCCTTGGATTCTCACCGTTGGAGCAAGCACCATTGACAGAAAAATAAGCTCAACAGCACAGCTTGGAGATTCTCCAAAATATTTTTACTCTGGTGAATCTTTATTCGATCCTAAAAATTTTAGCACTTCACCGTTGCTACCTTTTGTTTATCCAGGTGCAAATCCCAATAACCAAACTGAATTCTGTGCCTTGGGATCTCTTGTGAATGTTGAAGGGAAAGTAGTGGTGTGTGAGAGAGGTGGAGGAGTGGGAAGAACTGCCAAAGGGGAAGAAGTCAATAGAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCACTGATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGCATCAAAACCTATATAAACTCAACAACTACACCTATAGCCATAGTCTATTCCCCACTCACTGAAATCGGAGATGCACTTGCTCCAATGGTTGCTTCTTTTTCATCAAGAGGACCGAGCATAGCTAGCCCCGGAATTTTAAAACCTGACATAATTGGTCCTGGCATGGTGTTAACATCCTTGCTGCATGGCCTGTTTCGGTGGATAATGTCACAATATTTGACATTATTTCAGCATGATTTGATTTCAGGTACCTCAGTGTCATGCCCACACCTTAGTGGCATTGCAGCCTTGATCAAGAGTTCACACCCGGACTGGTCACCTGCTGCCATCAAATCCGCCATTATGACAACCGCTGATCTGCTAAACCTCGAAGGCAAGCCAAGTGTTGATAGATCAACTATCCCAGCAGACATCTATAGCACTGGCTCAGGCCATGTCAACCCTTCAAAAGCCAATGACCCGGGGCTCATCTATGACATAAAACCACAAGATTACATTCCTTACTTGTGTGGTCTGAACTACACTGACAATCAAATAGAGATGATCACCCAACAAAGAGTGGACTGCTCTGAAGTAGGAGTCACAGCTGAAGCGCAGCTAAACTATCCTTCAGTTTCCATCACAATAGGGTCCTATCAAACTCAGAATTACATAAGAACTGTGACAAATGTTGGCCCGGCTAATTCAACCTACAAATCGGAGATTTTGAAACCCCAGAAATGGGGTGTGAGTGTGAACATGGGTGTGACTCCTTCGGTGCTTACATTCACAGAGGTCAACCAGACCATTACATACAATGTGGAGTTCTCCACACAACTGGTTGGGATAGATGGTTCATTTTCACAGGGATATTTGAGATGGCTTTCTGACATGTATTCTGTATACAGCCCCATAGCTGTCATCTTTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

656

Amino Acids

69.91

Weight (kDa)

5.69

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 92 - 472 1.3e-36 Subtilase family
PA PF02225 279 - 352 3e-10 PA domain
fn3_6 PF17766 551 - 652 4.4e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1384
Acc36I ACCTGC 3 cut(s) 440, 1037, 1384
Acc65I GGTACC 1 cut(s) 1304
AccB1I GGYRCC 2 cut(s) 362, 1304
AccI GTMKAC 1 cut(s) 1938
AccII CGCG 1 cut(s) 578
AciI CCGC 3 cut(s) 38, 1392, 1407
AclWI GGATC 2 cut(s) 764, 865
AcoI YGGCCR 2 cut(s) 24, 423
AcsI RAATTY 5 cut(s) 262, 600, 778, 842, 1188
AcuI CTGAAG 3 cut(s) 1647, 1650, 1665
AcvI CACGTG 1 cut(s) 421
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 2 cut(s) 1017, 1306
AfiI CCNNNNNNNGG 3 cut(s) 22, 1776, 1777
AflIII ACRYGT 1 cut(s) 1926
AgsI TTSAA 8 cut(s) 214, 220, 593, 875, 1043, 1503, 1743, 1765
AjnI CCWGG 3 cut(s) 147, 814, 1213
AjuI GAANNNNNNNTTGG 2 cut(s) 1132, 1164
AleI CACNNNNGTG 1 cut(s) 1329
AloI GAACNNNNNNTCC 2 cut(s) 1873, 1905
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 2 cut(s) 764, 865
AlwNI CAGNNNCTG 1 cut(s) 398
Ama87I CYCGRG 1 cut(s) 1516
AoxI GGCC 8 cut(s) 24, 313, 423, 631, 660, 1243, 1486, 1729
ApeKI GCWGC 8 cut(s) 154, 341, 476, 956, 1237, 1340, 1379, 1651
ApoI RAATTY 5 cut(s) 262, 600, 778, 842, 1188
Asp718I GGTACC 1 cut(s) 1304
AspLEI GCGC 3 cut(s) 365, 578, 1651
AspS9I GGNCC 6 cut(s) 632, 661, 1166, 1211, 1686, 1730
AsuC2I CCSGG 5 cut(s) 1185, 1364, 1517, 1518, 1733
AsuHPI GGTGA 7 cut(s) 206, 317, 667, 767, 783, 1365, 1594
AsuNHI GCTAGC 1 cut(s) 1178
AvaI CYCGRG 1 cut(s) 1516
AvaII GGWCC 3 cut(s) 1166, 1211, 1686
BaeI ACNNNNGTAYC 2 cut(s) 1296, 1329
BalI TGGCCA 2 cut(s) 26, 425
BanI GGYRCC 2 cut(s) 362, 1304
BanII GRGCYC 1 cut(s) 1524
BbrPI CACGTG 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 8 cut(s) 141, 328, 488, 943, 1224, 1352, 1366, 1663
BccI CCATC 8 cut(s) 77, 434, 554, 561, 1391, 1682, 1880, 1908
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 3 cut(s) 149, 816, 1215
BclI TGATCA 2 cut(s) 1347, 1599
BcnI CCSGG 5 cut(s) 1185, 1364, 1517, 1518, 1733
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 4 cut(s) 333, 473, 968, 1179
BfmI CTRYAG 4 cut(s) 640, 991, 1089, 1471
BfoI RGCGCY 1 cut(s) 366
BfuAI ACCTGC 3 cut(s) 440, 1037, 1384
BisI GCNGC 9 cut(s) 39, 155, 342, 477, 957, 1238, 1341, 1380, 1652
BlsI GCNGC 9 cut(s) 40, 156, 343, 478, 958, 1239, 1342, 1381, 1653
Bme1390I CCNGG 8 cut(s) 149, 816, 1185, 1215, 1364, 1517, 1518, 1733
Bme18I GGWCC 3 cut(s) 1166, 1211, 1686
BmeT110I CYCGRG 1 cut(s) 1516
BmgT120I GGNCC 6 cut(s) 632, 661, 1166, 1211, 1686, 1730
BmiI GGNNCC 4 cut(s) 364, 663, 1306, 1687
BmrFI CCNGG 8 cut(s) 149, 816, 1185, 1215, 1364, 1517, 1518, 1733
BmsI GCATC 4 cut(s) 404, 997, 1065, 1117
BmtI GCTAGC 1 cut(s) 1182
Bpu10I CCTNAGC 2 cut(s) 1000, 1483
BpuMI CCSGG 5 cut(s) 1185, 1364, 1517, 1518, 1733
BsaAI YACGTR 1 cut(s) 421
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 9 cut(s) 148, 268, 316, 444, 665, 852, 1183, 1516, 1517
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 1776, 1777
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 3 cut(s) 1373, 1483, 1878
Bse3DI GCAATG 3 cut(s) 75, 402, 1335
BseBI CCWGG 3 cut(s) 149, 816, 1215
BseDI CCNNGG 9 cut(s) 148, 268, 316, 444, 665, 852, 1183, 1516, 1517
BseGI GGATG 3 cut(s) 100, 514, 1229
BseLI CCNNNNNNNGG 3 cut(s) 22, 1776, 1777
BseMI GCAATG 3 cut(s) 75, 402, 1335
BseMII CTCAG 4 cut(s) 555, 1323, 1497, 1712
BseNI ACTGG 3 cut(s) 1373, 1483, 1878
BseRI GAGGAG 1 cut(s) 921
BseXI GCAGC 8 cut(s) 141, 328, 488, 943, 1224, 1352, 1366, 1663
BseYI CCCAGC 1 cut(s) 1459
BsgI GTGCAG 1 cut(s) 424
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 8 cut(s) 26, 315, 425, 633, 662, 1245, 1488, 1731
BshNI GGYRCC 2 cut(s) 362, 1304
BsiHKAI GWGCWC 1 cut(s) 516
BsiHKCI CYCGRG 1 cut(s) 1516
BsiSI CCGG 5 cut(s) 30, 1185, 1364, 1517, 1733
BslI CCNNNNNNNGG 3 cut(s) 22, 1776, 1777
BsmAI GTCTC 1 cut(s) 298
BsnI GGCC 8 cut(s) 26, 315, 425, 633, 662, 1245, 1488, 1731
BsoBI CYCGRG 1 cut(s) 1516
Bsp1286I GDGCHC 2 cut(s) 516, 1524
Bsp143I GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
Bsp19I CCATGG 1 cut(s) 316
BspACI CCGC 3 cut(s) 38, 1392, 1407
BspANI GGCC 8 cut(s) 26, 315, 425, 633, 662, 1245, 1488, 1731
BspCNI CTCAG 4 cut(s) 554, 1322, 1496, 1711
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 4 cut(s) 364, 663, 1306, 1687
BspMI ACCTGC 3 cut(s) 440, 1037, 1384
BspOI GCTAGC 1 cut(s) 1182
BspPI GGATC 2 cut(s) 764, 865
BspT107I GGYRCC 2 cut(s) 362, 1304
BsrDI GCAATG 3 cut(s) 75, 402, 1335
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 3 cut(s) 1373, 1483, 1878
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 9 cut(s) 148, 268, 316, 444, 665, 852, 1183, 1516, 1517
BssMI GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
BssNAI GTATAC 1 cut(s) 1939
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 4 cut(s) 268, 316, 665, 852
Bst1107I GTATAC 1 cut(s) 1939
Bst2UI CCWGG 3 cut(s) 149, 816, 1215
Bst4CI ACNGT 6 cut(s) 194, 232, 522, 679, 795, 1717
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 3 cut(s) 413, 455, 953
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 5 cut(s) 96, 367, 688, 1180, 1433
BstDEI CTNAG 6 cut(s) 541, 1000, 1309, 1328, 1483, 1698
BstDSI CCRYGG 1 cut(s) 316
BstEII GGTNACC 1 cut(s) 1371
BstF5I GGATG 3 cut(s) 100, 514, 1229
BstFNI CGCG 1 cut(s) 578
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 3 cut(s) 365, 578, 1651
BstKTI GATC 6 cut(s) 772, 860, 1350, 1414, 1451, 1602
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
BstMWI GCNNNNNNNGC 7 cut(s) 413, 422, 455, 575, 584, 953, 1179
BstNI CCWGG 3 cut(s) 149, 816, 1215
BstNSI RCATGY 2 cut(s) 98, 1930
BstPI GGTNACC 1 cut(s) 1371
BstSCI CCNGG 8 cut(s) 147, 814, 1183, 1213, 1362, 1515, 1516, 1731
BstSFI CTRYAG 4 cut(s) 640, 991, 1089, 1471
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 8 cut(s) 141, 328, 488, 943, 1224, 1352, 1366, 1663
BstX2I RGATCY 1 cut(s) 857
BstXI CCANNNNNNTGG 2 cut(s) 560, 1873
BstYI RGATCY 1 cut(s) 857
BstZ17I GTATAC 1 cut(s) 1939
BsuRI GGCC 8 cut(s) 26, 315, 425, 633, 662, 1245, 1488, 1731
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 3 cut(s) 100, 514, 1229
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 8 cut(s) 237, 486, 518, 981, 1113, 1317, 1476, 1578
BveI ACCTGC 3 cut(s) 440, 1037, 1384
Cac8I GCNNGC 5 cut(s) 96, 367, 688, 1180, 1433
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 3 cut(s) 365, 578, 1651
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 6 cut(s) 632, 661, 1166, 1211, 1686, 1730
Cfr9I CCCGGG 1 cut(s) 1516
Csp6I GTAC 2 cut(s) 1016, 1305
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviQI GTAC 2 cut(s) 1016, 1305
DdeI CTNAG 6 cut(s) 541, 1000, 1309, 1328, 1483, 1698
DinI GGCGCC 1 cut(s) 364
DpnI GATC 6 cut(s) 771, 859, 1349, 1413, 1450, 1601
DpnII GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
DraI TTTAAA 1 cut(s) 1194
EaeI YGGCCR 2 cut(s) 24, 423
EciI GGCGGA 1 cut(s) 1381
Eco130I CCWWGG 4 cut(s) 268, 316, 665, 852
Eco24I GRGCYC 1 cut(s) 1524
Eco47I GGWCC 3 cut(s) 1166, 1211, 1686
Eco57I CTGAAG 3 cut(s) 1647, 1650, 1665
Eco72I CACGTG 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 1516
Eco91I GGTNACC 1 cut(s) 1371
EcoO109I RGGNCCY 2 cut(s) 661, 1686
EcoO65I GGTNACC 1 cut(s) 1371
EcoRI GAATTC 1 cut(s) 842
EcoRII CCWGG 3 cut(s) 147, 814, 1213
EcoT14I CCWWGG 4 cut(s) 268, 316, 665, 852
EcoT38I GRGCYC 1 cut(s) 1524
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 4 cut(s) 268, 316, 665, 852
FbaI TGATCA 2 cut(s) 1347, 1599
FblI GTMKAC 1 cut(s) 1938
Fnu4HI GCNGC 9 cut(s) 39, 155, 342, 477, 957, 1238, 1341, 1380, 1652
FokI GGATG 3 cut(s) 87, 521, 1216
FriOI GRGCYC 1 cut(s) 1524
Fsp4HI GCNGC 9 cut(s) 39, 155, 342, 477, 957, 1238, 1341, 1380, 1652
FspBI CTAG 4 cut(s) 333, 473, 968, 1179
GlaI GCGC 3 cut(s) 364, 577, 1650
GluI GCNGC 9 cut(s) 39, 155, 342, 477, 957, 1238, 1341, 1380, 1652
GsaI CCCAGC 1 cut(s) 1463
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 8 cut(s) 26, 315, 425, 633, 662, 1245, 1488, 1731
HapII CCGG 5 cut(s) 30, 1185, 1364, 1517, 1733
HhaI GCGC 3 cut(s) 365, 578, 1651
Hin1I GRCGYC 1 cut(s) 363
Hin6I GCGC 3 cut(s) 363, 576, 1649
HinP1I GCGC 3 cut(s) 363, 576, 1649
HincII GTYRAC 3 cut(s) 1227, 1495, 1834
HindII GTYRAC 3 cut(s) 1227, 1495, 1834
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 6 cut(s) 670, 731, 758, 964, 1635, 1804
HpaI GTTAAC 1 cut(s) 1227
HpaII CCGG 5 cut(s) 30, 1185, 1364, 1517, 1733
HphI GGTGA 7 cut(s) 206, 317, 667, 767, 783, 1365, 1594
Hpy166II GTNNAC 9 cut(s) 7, 973, 1227, 1358, 1495, 1618, 1792, 1834, 1939
Hpy188I TCNGA 8 cut(s) 170, 225, 1124, 1572, 1627, 1701, 1756, 1924
Hpy188III TCNNGA 6 cut(s) 104, 482, 557, 1161, 1351, 1968
Hpy8I GTNNAC 9 cut(s) 7, 973, 1227, 1358, 1495, 1618, 1792, 1834, 1939
HpyAV CCTTC 7 cut(s) 191, 305, 869, 1421, 1509, 1674, 1818
HpyCH4III ACNGT 6 cut(s) 194, 232, 522, 679, 795, 1717
HpyCH4IV ACGT 1 cut(s) 420
HpyF10VI GCNNNNNNNGC 7 cut(s) 413, 422, 455, 575, 584, 953, 1179
HpyF3I CTNAG 6 cut(s) 541, 1000, 1309, 1328, 1483, 1698
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 1 cut(s) 363
HspAI GCGC 3 cut(s) 363, 576, 1649
KasI GGCGCC 1 cut(s) 362
KpnI GGTACC 1 cut(s) 1308
Ksp22I TGATCA 2 cut(s) 1347, 1599
KspAI GTTAAC 1 cut(s) 1227
Kzo9I GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
LmnI GCTCC 3 cut(s) 584, 683, 1141
Lsp1109I GCAGC 8 cut(s) 141, 328, 488, 943, 1224, 1352, 1366, 1663
LweI GCATC 4 cut(s) 404, 997, 1065, 1117
MaeI CTAG 4 cut(s) 333, 473, 968, 1179
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 6 cut(s) 464, 1263, 1371, 1636, 1717, 1801
MalI GATC 6 cut(s) 771, 859, 1349, 1413, 1450, 1601
MboI GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
MboII GAAGA 3 cut(s) 320, 927, 944
MflI RGATCY 1 cut(s) 857
MhlI GDGCHC 2 cut(s) 516, 1524
MlsI TGGCCA 2 cut(s) 26, 425
MluNI TGGCCA 2 cut(s) 26, 425
Mly113I GGCGCC 1 cut(s) 363
MlyI GAGTC 2 cut(s) 1644, 1798
MmeI TCCRAC 1 cut(s) 661
Mox20I TGGCCA 2 cut(s) 26, 425
MscI TGGCCA 2 cut(s) 26, 425
MseI TTAA 2 cut(s) 1193, 1226
MslI CAYNNNNRTG 3 cut(s) 558, 1329, 1799
Msp20I TGGCCA 2 cut(s) 26, 425
MspA1I CMGCKG 4 cut(s) 612, 620, 1409, 1643
MspI CCGG 5 cut(s) 30, 1185, 1364, 1517, 1733
MspR9I CCNGG 8 cut(s) 149, 816, 1185, 1215, 1364, 1517, 1518, 1733
MvaI CCWGG 3 cut(s) 149, 816, 1215
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 7 cut(s) 413, 422, 455, 575, 584, 953, 1179
NarI GGCGCC 1 cut(s) 363
NciI CCSGG 5 cut(s) 1185, 1364, 1517, 1518, 1733
NcoI CCATGG 1 cut(s) 316
NdeII GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
NheI GCTAGC 1 cut(s) 1178
NlaIV GGNNCC 4 cut(s) 364, 663, 1306, 1687
NmuCI GTSAC 6 cut(s) 464, 1263, 1371, 1636, 1717, 1801
NspI RCATGY 2 cut(s) 98, 1930
OliI CACNNNNGTG 1 cut(s) 1329
PaeI GCATGC 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 1384
PciI ACATGT 1 cut(s) 1926
PfeI GAWTC 4 cut(s) 670, 731, 758, 964
PkrI GCNGC 9 cut(s) 40, 156, 343, 478, 958, 1239, 1342, 1381, 1653
PleI GAGTC 2 cut(s) 1643, 1798
PluTI GGCGCC 1 cut(s) 366
PmaCI CACGTG 1 cut(s) 421
PmlI CACGTG 1 cut(s) 421
PpsI GAGTC 2 cut(s) 1643, 1798
Ppu21I YACGTR 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 1686
PscI ACATGT 1 cut(s) 1926
Psp5II RGGWCCY 1 cut(s) 1686
Psp6I CCWGG 3 cut(s) 147, 814, 1213
PspCI CACGTG 1 cut(s) 421
PspEI GGTNACC 1 cut(s) 1371
PspFI CCCAGC 1 cut(s) 1459
PspGI CCWGG 3 cut(s) 147, 814, 1213
PspN4I GGNNCC 4 cut(s) 364, 663, 1306, 1687
PspPI GGNCC 6 cut(s) 632, 661, 1166, 1211, 1686, 1730
PspPPI RGGWCCY 1 cut(s) 1686
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 1 cut(s) 857
PvuII CAGCTG 3 cut(s) 612, 620, 1643
RsaI GTAC 2 cut(s) 1017, 1306
RsaNI GTAC 2 cut(s) 1016, 1305
RseI CAYNNNNRTG 3 cut(s) 558, 1329, 1799
SaqAI TTAA 2 cut(s) 1193, 1226
SatI GCNGC 9 cut(s) 39, 155, 342, 477, 957, 1238, 1341, 1380, 1652
Sau3AI GATC 6 cut(s) 769, 857, 1347, 1411, 1448, 1599
Sau96I GGNCC 6 cut(s) 632, 661, 1166, 1211, 1686, 1730
SchI GAGTC 2 cut(s) 1644, 1798
ScrFI CCNGG 8 cut(s) 149, 816, 1185, 1215, 1364, 1517, 1518, 1733
SduI GDGCHC 2 cut(s) 516, 1524
SfaNI GCATC 4 cut(s) 404, 997, 1065, 1117
SfcI CTRYAG 4 cut(s) 640, 991, 1089, 1471
SfoI GGCGCC 1 cut(s) 364
SinI GGWCC 3 cut(s) 1166, 1211, 1686
SmaI CCCGGG 1 cut(s) 1518
SmiMI CAYNNNNRTG 3 cut(s) 558, 1329, 1799
SphI GCATGC 1 cut(s) 98
SsiI CCGC 3 cut(s) 38, 1392, 1407
SspDI GGCGCC 1 cut(s) 362
SspI AATATT 2 cut(s) 743, 1271
SspMI CTAG 4 cut(s) 333, 473, 968, 1179
StyD4I CCNGG 8 cut(s) 147, 814, 1183, 1213, 1362, 1515, 1516, 1731
StyI CCWWGG 4 cut(s) 268, 316, 665, 852
TaaI ACNGT 6 cut(s) 194, 232, 522, 679, 795, 1717
TaiI ACGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 768, 1425
TaqII GACCGA 1 cut(s) 1183
TatI WGTACW 1 cut(s) 1015
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 4 cut(s) 670, 731, 758, 964
Tru1I TTAA 2 cut(s) 1193, 1226
Tru9I TTAA 2 cut(s) 1193, 1226
TscAI CASTG 8 cut(s) 237, 493, 525, 988, 1120, 1317, 1483, 1585
TseFI GTSAC 6 cut(s) 464, 1263, 1371, 1636, 1717, 1801
TseI GCWGC 8 cut(s) 154, 341, 476, 956, 1237, 1340, 1379, 1651
Tsp45I GTSAC 6 cut(s) 464, 1263, 1371, 1636, 1717, 1801
TspDTI ATGAA 2 cut(s) 1146, 1881
TspMI CCCGGG 1 cut(s) 1516
TspRI CASTG 8 cut(s) 237, 493, 525, 988, 1120, 1317, 1483, 1585
VpaK11BI GGWCC 3 cut(s) 1166, 1211, 1686
XapI RAATTY 5 cut(s) 262, 600, 778, 842, 1188
XceI RCATGY 2 cut(s) 98, 1930
XmaI CCCGGG 1 cut(s) 1516
XmiI GTMKAC 1 cut(s) 1938
XspI CTAG 4 cut(s) 333, 473, 968, 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.